Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0616430 | XP_039684839.1 | 97.590 | 332 | 8 | 0 | 1 | 332 | 1 | 332 | 0.0 | 684 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0616430 | sp|Q38909|XTH28_ARATH | 68.502 | 327 | 99 | 3 | 6 | 331 | 8 | 331 | 2.04e-167 | 472 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0616430 | G8A358 | 97.590 | 332 | 8 | 0 | 1 | 332 | 1 | 332 | 0.0 | 684 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0616430 | Msa0655900 | 0.865561 | 5.399375e-65 | -8.615850e-47 |
| Msa0616430 | Msa0699760 | 0.995386 | 9.475408e-216 | -8.615850e-47 |
| Msa0465470 | Msa0616430 | 0.800305 | 1.533825e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0616430 | MtrunA17_Chr8g0375981 | 97.590 | 332 | 8 | 0 | 1 | 332 | 1 | 332 | 0.0 | 684 |
| Msa0616430 | MtrunA17_Chr1g0161951 | 77.429 | 319 | 70 | 2 | 6 | 323 | 7 | 324 | 0.0 | 532 |
| Msa0616430 | MtrunA17_Chr5g0410621 | 56.949 | 295 | 119 | 3 | 20 | 308 | 31 | 323 | 3.42e-124 | 360 |
| Msa0616430 | MtrunA17_Chr8g0382041 | 48.936 | 282 | 137 | 5 | 25 | 302 | 28 | 306 | 9.56e-96 | 287 |
| Msa0616430 | MtrunA17_Chr8g0365231 | 45.425 | 306 | 139 | 5 | 7 | 290 | 11 | 310 | 2.74e-89 | 270 |
| Msa0616430 | MtrunA17_Chr4g0028221 | 44.853 | 272 | 138 | 6 | 22 | 290 | 42 | 304 | 1.14e-71 | 224 |
| Msa0616430 | MtrunA17_Chr1g0180451 | 40.468 | 299 | 153 | 7 | 8 | 290 | 5 | 294 | 2.81e-66 | 210 |
| Msa0616430 | MtrunA17_Chr2g0327411 | 39.865 | 296 | 159 | 7 | 6 | 290 | 22 | 309 | 1.12e-65 | 209 |
| Msa0616430 | MtrunA17_Chr7g0264731 | 38.384 | 297 | 160 | 6 | 9 | 290 | 3 | 291 | 2.30e-61 | 197 |
| Msa0616430 | MtrunA17_Chr7g0252601 | 36.972 | 284 | 171 | 5 | 9 | 290 | 10 | 287 | 1.02e-60 | 196 |
| Msa0616430 | MtrunA17_Chr1g0206081 | 36.149 | 296 | 183 | 5 | 4 | 296 | 8 | 300 | 1.13e-59 | 193 |
| Msa0616430 | MtrunA17_Chr6g0461761 | 36.824 | 296 | 174 | 8 | 1 | 291 | 1 | 288 | 9.39e-59 | 191 |
| Msa0616430 | MtrunA17_Chr3g0096651 | 40.613 | 261 | 146 | 5 | 31 | 290 | 30 | 282 | 4.54e-58 | 189 |
| Msa0616430 | MtrunA17_Chr1g0206681 | 37.358 | 265 | 158 | 4 | 31 | 292 | 34 | 293 | 6.15e-58 | 189 |
| Msa0616430 | MtrunA17_Chr4g0072611 | 36.949 | 295 | 166 | 8 | 1 | 290 | 1 | 280 | 1.06e-57 | 188 |
| Msa0616430 | MtrunA17_Chr4g0023961 | 38.095 | 294 | 156 | 10 | 9 | 291 | 11 | 289 | 1.48e-57 | 187 |
| Msa0616430 | MtrunA17_Chr2g0323161 | 37.667 | 300 | 161 | 11 | 4 | 291 | 2 | 287 | 1.85e-57 | 187 |
| Msa0616430 | MtrunA17_Chr8g0340001 | 40.909 | 242 | 128 | 7 | 55 | 291 | 1 | 232 | 1.92e-57 | 186 |
| Msa0616430 | MtrunA17_Chr5g0443391 | 38.168 | 262 | 149 | 5 | 31 | 290 | 28 | 278 | 4.44e-57 | 186 |
| Msa0616430 | MtrunA17_Chr7g0252631 | 37.282 | 287 | 166 | 6 | 9 | 290 | 10 | 287 | 5.93e-57 | 186 |
| Msa0616430 | MtrunA17_Chr7g0252621 | 37.282 | 287 | 166 | 6 | 9 | 290 | 10 | 287 | 9.72e-57 | 186 |
| Msa0616430 | MtrunA17_Chr7g0258911 | 34.694 | 294 | 177 | 5 | 1 | 292 | 1 | 281 | 3.52e-56 | 184 |
| Msa0616430 | MtrunA17_Chr6g0461731 | 35.640 | 289 | 174 | 7 | 7 | 291 | 31 | 311 | 6.73e-56 | 184 |
| Msa0616430 | MtrunA17_Chr4g0071341 | 35.472 | 265 | 162 | 5 | 31 | 292 | 25 | 283 | 2.22e-54 | 179 |
| Msa0616430 | MtrunA17_Chr4g0072601 | 36.719 | 256 | 154 | 4 | 39 | 292 | 36 | 285 | 2.90e-54 | 179 |
| Msa0616430 | MtrunA17_Chr4g0054451 | 35.932 | 295 | 172 | 9 | 3 | 291 | 7 | 290 | 8.25e-54 | 178 |
| Msa0616430 | MtrunA17_Chr4g0054431 | 36.934 | 287 | 164 | 9 | 11 | 291 | 13 | 288 | 1.26e-53 | 177 |
| Msa0616430 | MtrunA17_Chr4g0072671 | 35.955 | 267 | 159 | 5 | 31 | 291 | 26 | 286 | 8.68e-52 | 172 |
| Msa0616430 | MtrunA17_Chr3g0122021 | 36.015 | 261 | 155 | 5 | 31 | 290 | 28 | 277 | 1.40e-51 | 172 |
| Msa0616430 | MtrunA17_Chr3g0122031 | 36.015 | 261 | 155 | 5 | 31 | 290 | 28 | 277 | 1.41e-51 | 172 |
| Msa0616430 | MtrunA17_Chr2g0299061 | 34.694 | 294 | 166 | 7 | 8 | 291 | 10 | 287 | 2.66e-50 | 169 |
| Msa0616430 | MtrunA17_Chr8g0377841 | 34.733 | 262 | 160 | 4 | 30 | 290 | 30 | 281 | 6.19e-50 | 167 |
| Msa0616430 | MtrunA17_Chr2g0303861 | 32.975 | 279 | 173 | 8 | 1 | 276 | 1 | 268 | 2.76e-49 | 166 |
| Msa0616430 | MtrunA17_Chr6g0486581 | 35.628 | 247 | 149 | 5 | 49 | 290 | 42 | 283 | 8.71e-49 | 165 |
| Msa0616430 | MtrunA17_Chr8g0389641 | 32.180 | 289 | 144 | 5 | 3 | 290 | 5 | 242 | 1.08e-45 | 155 |
| Msa0616430 | MtrunA17_Chr2g0303721 | 31.900 | 279 | 169 | 8 | 1 | 276 | 1 | 261 | 1.19e-43 | 151 |
| Msa0616430 | MtrunA17_Chr4g0072681 | 35.714 | 224 | 131 | 5 | 74 | 290 | 26 | 243 | 4.35e-43 | 149 |
| Msa0616430 | MtrunA17_Chr7g0235541 | 38.424 | 203 | 117 | 5 | 80 | 279 | 3 | 200 | 8.85e-42 | 144 |
| Msa0616430 | MtrunA17_Chr8g0390201 | 36.842 | 190 | 113 | 4 | 106 | 290 | 22 | 209 | 1.64e-37 | 133 |
| Msa0616430 | MtrunA17_Chr8g0350711 | 36.158 | 177 | 101 | 6 | 52 | 226 | 53 | 219 | 5.02e-20 | 87.4 |
| Msa0616430 | MtrunA17_Chr4g0072641 | 29.921 | 127 | 74 | 2 | 166 | 291 | 37 | 149 | 1.57e-16 | 75.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0616430 | AT1G14720.1 | 68.502 | 327 | 99 | 3 | 6 | 331 | 8 | 331 | 2.07e-168 | 472 |
| Msa0616430 | AT2G01850.1 | 68.997 | 329 | 95 | 5 | 6 | 330 | 8 | 333 | 1.05e-167 | 470 |
| Msa0616430 | AT1G32170.1 | 56.410 | 312 | 128 | 5 | 2 | 309 | 5 | 312 | 5.01e-125 | 362 |
| Msa0616430 | AT4G18990.1 | 52.104 | 309 | 130 | 4 | 25 | 319 | 34 | 338 | 4.82e-110 | 325 |
| Msa0616430 | AT1G10550.1 | 46.324 | 272 | 131 | 4 | 30 | 290 | 42 | 309 | 5.32e-83 | 254 |
| Msa0616430 | AT4G18990.2 | 51.477 | 237 | 97 | 4 | 97 | 319 | 32 | 264 | 6.07e-82 | 250 |
| Msa0616430 | AT4G13090.1 | 44.138 | 290 | 147 | 8 | 11 | 293 | 10 | 291 | 1.59e-73 | 229 |
| Msa0616430 | AT4G13080.1 | 41.509 | 265 | 148 | 4 | 29 | 293 | 37 | 294 | 1.26e-70 | 222 |
| Msa0616430 | AT3G44990.1 | 40.590 | 271 | 150 | 5 | 23 | 290 | 31 | 293 | 1.34e-64 | 206 |
| Msa0616430 | AT3G25050.1 | 39.700 | 267 | 148 | 7 | 28 | 290 | 32 | 289 | 1.86e-63 | 203 |
| Msa0616430 | AT5G13870.2 | 38.754 | 289 | 162 | 7 | 6 | 290 | 10 | 287 | 1.87e-63 | 203 |
| Msa0616430 | AT5G13870.1 | 38.754 | 289 | 162 | 7 | 6 | 290 | 10 | 287 | 1.87e-63 | 203 |
| Msa0616430 | AT5G13870.3 | 38.754 | 289 | 162 | 7 | 6 | 290 | 10 | 287 | 1.87e-63 | 203 |
| Msa0616430 | AT1G11545.1 | 38.514 | 296 | 173 | 7 | 4 | 294 | 12 | 303 | 1.26e-62 | 201 |
| Msa0616430 | AT2G36870.1 | 38.689 | 305 | 164 | 6 | 1 | 290 | 1 | 297 | 2.11e-62 | 201 |
| Msa0616430 | AT2G06850.1 | 37.931 | 290 | 171 | 6 | 3 | 290 | 8 | 290 | 5.09e-59 | 192 |
| Msa0616430 | AT4G37800.1 | 38.194 | 288 | 157 | 9 | 9 | 290 | 17 | 289 | 4.51e-58 | 189 |
| Msa0616430 | AT4G25810.1 | 36.913 | 298 | 164 | 8 | 1 | 291 | 4 | 284 | 4.61e-57 | 186 |
| Msa0616430 | AT5G65730.1 | 36.552 | 290 | 173 | 7 | 2 | 290 | 11 | 290 | 1.38e-56 | 186 |
| Msa0616430 | AT5G57550.1 | 36.713 | 286 | 167 | 8 | 10 | 290 | 6 | 282 | 8.70e-56 | 183 |
| Msa0616430 | AT2G06850.2 | 42.489 | 233 | 126 | 5 | 60 | 290 | 4 | 230 | 1.10e-55 | 181 |
| Msa0616430 | AT5G57560.1 | 36.000 | 300 | 165 | 9 | 1 | 291 | 1 | 282 | 1.16e-55 | 183 |
| Msa0616430 | AT2G14620.1 | 36.301 | 292 | 172 | 6 | 6 | 292 | 14 | 296 | 2.58e-54 | 180 |
| Msa0616430 | AT4G30270.1 | 35.789 | 285 | 157 | 6 | 9 | 291 | 6 | 266 | 5.37e-54 | 178 |
| Msa0616430 | AT3G23730.1 | 35.714 | 266 | 161 | 4 | 30 | 290 | 26 | 286 | 3.04e-53 | 177 |
| Msa0616430 | AT4G03210.1 | 37.121 | 264 | 154 | 6 | 31 | 290 | 29 | 284 | 3.71e-53 | 177 |
| Msa0616430 | AT4G14130.1 | 36.525 | 282 | 165 | 6 | 11 | 290 | 15 | 284 | 4.05e-53 | 176 |
| Msa0616430 | AT4G30280.1 | 37.979 | 287 | 162 | 7 | 7 | 290 | 8 | 281 | 1.45e-52 | 175 |
| Msa0616430 | AT5G48070.1 | 38.272 | 243 | 138 | 5 | 50 | 290 | 49 | 281 | 1.79e-52 | 174 |
| Msa0616430 | AT4G30290.1 | 37.979 | 287 | 162 | 7 | 7 | 290 | 3 | 276 | 3.09e-52 | 174 |
| Msa0616430 | AT5G57530.1 | 35.581 | 267 | 162 | 6 | 30 | 293 | 26 | 285 | 5.45e-52 | 173 |
| Msa0616430 | AT5G57540.1 | 34.831 | 267 | 164 | 6 | 30 | 293 | 25 | 284 | 2.37e-51 | 172 |
| Msa0616430 | AT2G18800.1 | 35.125 | 279 | 154 | 5 | 31 | 291 | 28 | 297 | 3.61e-51 | 172 |
| Msa0616430 | AT1G65310.1 | 39.062 | 256 | 143 | 6 | 39 | 291 | 37 | 282 | 7.75e-51 | 170 |
| Msa0616430 | AT1G65310.2 | 39.062 | 256 | 143 | 6 | 39 | 291 | 59 | 304 | 1.21e-50 | 171 |
| Msa0616430 | AT4G03210.2 | 37.302 | 252 | 147 | 6 | 44 | 290 | 11 | 256 | 8.70e-50 | 167 |
| Msa0616430 | AT2G14620.2 | 39.407 | 236 | 131 | 5 | 62 | 292 | 18 | 246 | 2.47e-49 | 166 |
| Msa0616430 | AT4G28850.1 | 33.898 | 295 | 177 | 7 | 6 | 290 | 1 | 287 | 2.76e-49 | 167 |
| Msa0616430 | AT2G14620.3 | 39.648 | 227 | 125 | 5 | 71 | 292 | 16 | 235 | 6.95e-48 | 161 |
| Msa0616430 | AT4G25820.1 | 34.091 | 264 | 166 | 4 | 31 | 292 | 30 | 287 | 3.15e-47 | 161 |
| Msa0616430 | AT3G48580.1 | 33.471 | 242 | 134 | 10 | 53 | 290 | 55 | 273 | 6.21e-35 | 129 |
| Msa0616430 | AT3G48580.3 | 33.761 | 234 | 128 | 10 | 61 | 290 | 4 | 214 | 4.47e-33 | 122 |
| Msa0616430 | AT2G36870.2 | 38.333 | 180 | 94 | 3 | 1 | 165 | 1 | 178 | 1.58e-31 | 117 |
| Msa0616430 | AT3G48580.2 | 29.752 | 242 | 123 | 11 | 53 | 290 | 55 | 253 | 2.63e-21 | 92.0 |
Find 111 sgRNAs with CRISPR-Local
Find 200 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGATTCAGACCAATGTTTA+TGG | 0.234371 | 4_2:-66227348 | Msa0616430:CDS |
| ATCTAGAAATTTACCTATTA+CGG | 0.254701 | 4_2:-66228201 | Msa0616430:CDS |
| TAGGACAAGGTCGGAGAATT+TGG | 0.260987 | 4_2:+66226263 | None:intergenic |
| TGACTCTGTATGCGACTATA+TGG | 0.266092 | 4_2:-66226353 | Msa0616430:CDS |
| TCTCATGATATTTACCTTCA+TGG | 0.300530 | 4_2:-66227965 | Msa0616430:CDS |
| GTCCGTGTAACTTCTCTAAT+AGG | 0.305543 | 4_2:+66226411 | None:intergenic |
| GAAGGGTACACACCTTTGTT+TGG | 0.310448 | 4_2:-66228170 | Msa0616430:CDS |
| AAGAATAAAACAATACTATT+AGG | 0.310845 | 4_2:+66225568 | None:intergenic |
| ATTGAAAACCCTGAGGTATA+TGG | 0.338504 | 4_2:+66225664 | None:intergenic |
| AAGGGTACACACCTTTGTTT+GGG | 0.339045 | 4_2:-66228169 | Msa0616430:CDS |
| CCTTCATCAAAGGCCGTAAT+AGG | 0.340035 | 4_2:+66228188 | None:intergenic |
| ATTCATGGGTTATTCTCATA+TGG | 0.345426 | 4_2:-66228270 | Msa0616430:exon |
| AAATCAAAATGGAGAACTTC+AGG | 0.359114 | 4_2:-66226146 | Msa0616430:CDS |
| TCCAGAGAATACTGTATTGA+TGG | 0.361854 | 4_2:+66227259 | None:intergenic |
| AGTTCCAAATCCATTCCTTC+TGG | 0.389842 | 4_2:-66226186 | Msa0616430:CDS |
| CTTACTGTTACGACAAAATC+AGG | 0.399238 | 4_2:-66226104 | Msa0616430:CDS |
| AACACCAGAAGGAATGGATT+TGG | 0.411428 | 4_2:+66226182 | None:intergenic |
| GATTAATTTCATCCTTGATT+CGG | 0.413783 | 4_2:-66225909 | Msa0616430:three_prime_UTR |
| TAGATAGAAAGAGAGAGGTT+TGG | 0.416524 | 4_2:+66228396 | None:intergenic |
| GAAACATTAGAGGCAAAGAT+TGG | 0.421060 | 4_2:-66227371 | Msa0616430:CDS |
| CCTATTACGGCCTTTGATGA+AGG | 0.421584 | 4_2:-66228188 | Msa0616430:CDS |
| CATGAGTTATTTAATGAAGT+AGG | 0.439554 | 4_2:-66225630 | Msa0616430:three_prime_UTR |
| CTGCCGCTGCCGAATGTGAC+AGG | 0.447195 | 4_2:+66226012 | None:intergenic |
| CTTAGCCTTTCAGCTTCTTG+TGG | 0.450432 | 4_2:+66226045 | None:intergenic |
| GAGGTATATGGTCTAAATAC+AGG | 0.451985 | 4_2:+66225676 | None:intergenic |
| TACAGAGTCATAGGCTTGGC+AGG | 0.452062 | 4_2:+66226366 | None:intergenic |
| CTCATGATATTTACCTTCAT+GGG | 0.454019 | 4_2:-66227964 | Msa0616430:CDS |
| TCTCTATACACCACGAAGAA+AGG | 0.459945 | 4_2:+66225855 | None:intergenic |
| CTTTCTTTAGATGAAAGAAC+AGG | 0.466457 | 4_2:-66228104 | Msa0616430:intron |
| TCTTTCCTCTCTGCCAATGC+TGG | 0.468353 | 4_2:+66227314 | None:intergenic |
| GTATTTAGACCATATACCTC+AGG | 0.468384 | 4_2:-66225673 | Msa0616430:three_prime_UTR |
| ATGTGTATAGTTCATACCTT+AGG | 0.468895 | 4_2:-66225757 | Msa0616430:three_prime_UTR |
| TCCATCAATACAGTATTCTC+TGG | 0.470704 | 4_2:-66227260 | Msa0616430:CDS |
| TTCTCCGACCTTGTCCTACA+TGG | 0.473548 | 4_2:-66226258 | Msa0616430:CDS |
| TCGGAGAATTTGGCAACATA+TGG | 0.479047 | 4_2:+66226273 | None:intergenic |
| ATAGTCGCATACAGAGTCAT+AGG | 0.480165 | 4_2:+66226357 | None:intergenic |
| CATCAGATTGGGCTACCAAT+GGG | 0.480813 | 4_2:-66226326 | Msa0616430:CDS |
| GATTCGGTTCGATTACCACC+TGG | 0.482451 | 4_2:-66225893 | Msa0616430:three_prime_UTR |
| AACGTCTTTGTTCATAGAGA+TGG | 0.488359 | 4_2:-66228140 | Msa0616430:CDS |
| ACAAAATACCTGGGAACCTA+AGG | 0.498007 | 4_2:+66225741 | None:intergenic |
| CAGACCAATGTTTATGGCAA+TGG | 0.498239 | 4_2:-66227342 | Msa0616430:CDS |
| AAATTCGACCCTGTCACATT+CGG | 0.498531 | 4_2:-66226021 | Msa0616430:CDS |
| AGTTCATACCTTAGGTTCCC+AGG | 0.502051 | 4_2:-66225749 | Msa0616430:three_prime_UTR |
| GCATCAGATTGGGCTACCAA+TGG | 0.503583 | 4_2:-66226327 | Msa0616430:CDS |
| GAGAGGAAAGATATGGTCTC+TGG | 0.504612 | 4_2:-66227302 | Msa0616430:CDS |
| TATAGAGAGAGAAAGAAAGA+GGG | 0.505042 | 4_2:-66225840 | Msa0616430:three_prime_UTR |
| ACTCAAAATCTATTTCATCA+TGG | 0.506747 | 4_2:+66227400 | None:intergenic |
| CACAAGAAGCTGAAAGGCTA+AGG | 0.507826 | 4_2:-66226044 | Msa0616430:CDS |
| CACTTGGCTACATGCTCGAT+TGG | 0.512580 | 4_2:+66226222 | None:intergenic |
| CTATATGGGATGCATCAGAT+TGG | 0.512671 | 4_2:-66226338 | Msa0616430:CDS |
| GACTCTGTATGCGACTATAT+GGG | 0.515315 | 4_2:-66226352 | Msa0616430:CDS |
| GTATAGAGAGAGAAAGAAAG+AGG | 0.517651 | 4_2:-66225841 | Msa0616430:three_prime_UTR |
| AAAGATAGATAGAAAGAGAG+AGG | 0.519824 | 4_2:+66228391 | None:intergenic |
| CTATTACGGCCTTTGATGAA+GGG | 0.522479 | 4_2:-66228187 | Msa0616430:CDS |
| GACATGAATAACAAAATACC+TGG | 0.525056 | 4_2:+66225731 | None:intergenic |
| TATATGGGATGCATCAGATT+GGG | 0.525648 | 4_2:-66226337 | Msa0616430:CDS |
| TGGTGTAACACCAGAAGGAA+TGG | 0.534559 | 4_2:+66226176 | None:intergenic |
| TATTGATGGAAATCCTCTGC+AGG | 0.536591 | 4_2:+66227273 | None:intergenic |
| GGCAATGGTAGCACCAGCAT+TGG | 0.536609 | 4_2:-66227327 | Msa0616430:CDS |
| ATTCGGTTCGATTACCACCT+GGG | 0.536843 | 4_2:-66225892 | Msa0616430:three_prime_UTR |
| TTGTATCTGCAGATGTCAAA+TGG | 0.539892 | 4_2:-66227441 | Msa0616430:intron |
| AGCGATAGAGAGGTGCTAGC+TGG | 0.541569 | 4_2:+66228441 | None:intergenic |
| GTGGTAATCGAACCGAATCA+AGG | 0.543457 | 4_2:+66225897 | None:intergenic |
| GAGGAGAAAGGCAGAGACAA+AGG | 0.544653 | 4_2:+66228472 | None:intergenic |
| AAACAGGGTAACGAAGAAGA+AGG | 0.545127 | 4_2:+66228362 | None:intergenic |
| CGTGGTTTCCTCTGCTGTGG+TGG | 0.546130 | 4_2:+66225968 | None:intergenic |
| CTGGTTATTAGAGAGGAGAA+AGG | 0.550010 | 4_2:+66228460 | None:intergenic |
| CGCATACAGAGTCATAGGCT+TGG | 0.550129 | 4_2:+66226362 | None:intergenic |
| TGTTCATAGAGATGGCAAAT+CGG | 0.550991 | 4_2:-66228132 | Msa0616430:CDS |
| TGTACTGGTGTAACACCAGA+AGG | 0.555810 | 4_2:+66226171 | None:intergenic |
| GACCCTGTCACATTCGGCAG+CGG | 0.558184 | 4_2:-66226015 | Msa0616430:CDS |
| ACATTAGAGGCAAAGATTGG+AGG | 0.561664 | 4_2:-66227368 | Msa0616430:CDS |
| GGATTGATCACGCACTCTGA+TGG | 0.565213 | 4_2:+66226066 | None:intergenic |
| AAGCACTGAAATACCCATGA+AGG | 0.569254 | 4_2:+66227951 | None:intergenic |
| AACAGCTAGAAGCGATAGAG+AGG | 0.569345 | 4_2:+66228431 | None:intergenic |
| ATTTCTAGATGAAGCAGAAG+CGG | 0.570082 | 4_2:+66228214 | None:intergenic |
| ATTGGCAGAGAGGAAAGATA+TGG | 0.574281 | 4_2:-66227309 | Msa0616430:CDS |
| ACTTTGAGCAGTGTCACACT+TGG | 0.575287 | 4_2:+66226206 | None:intergenic |
| CTGCGTGGTTTCCTCTGCTG+TGG | 0.577797 | 4_2:+66225965 | None:intergenic |
| TTACCTGCTGATTACACTGC+CGG | 0.582709 | 4_2:-66227920 | Msa0616430:CDS |
| ATAGAGAGAGAAAGAAAGAG+GGG | 0.583848 | 4_2:-66225839 | Msa0616430:three_prime_UTR |
| TATTTAGACCATATACCTCA+GGG | 0.591028 | 4_2:-66225672 | Msa0616430:three_prime_UTR |
| ACACCGGCAGTGTAATCAGC+AGG | 0.593919 | 4_2:+66227917 | None:intergenic |
| AGGTGTGTACCCTTCATCAA+AGG | 0.593957 | 4_2:+66228178 | None:intergenic |
| GCTACCATTGCCATAAACAT+TGG | 0.602231 | 4_2:+66227338 | None:intergenic |
| TCTCTGGTTTGATCCTGCAG+AGG | 0.605207 | 4_2:-66227286 | Msa0616430:CDS |
| GTGCTAGCTGGTTATTAGAG+AGG | 0.605259 | 4_2:+66228453 | None:intergenic |
| AAACGACACCACCACAGCAG+AGG | 0.605813 | 4_2:-66225976 | Msa0616430:CDS |
| TCAATCCACAAGAAGCTGAA+AGG | 0.606328 | 4_2:-66226050 | Msa0616430:CDS |
| AGTTACACGGACAAAATCGA+TGG | 0.608259 | 4_2:-66226400 | Msa0616430:CDS |
| TCAGATTGGGCTACCAATGG+GGG | 0.608759 | 4_2:-66226324 | Msa0616430:CDS |
| GATCGACTGCACAACCATGT+AGG | 0.610773 | 4_2:+66226244 | None:intergenic |
| TGCCGCTGCCGAATGTGACA+GGG | 0.625854 | 4_2:+66226013 | None:intergenic |
| GGATGAAATTAATCTTCGTG+TGG | 0.627643 | 4_2:+66225918 | None:intergenic |
| ATCAGATTGGGCTACCAATG+GGG | 0.630175 | 4_2:-66226325 | Msa0616430:CDS |
| TTGATCACGCACTCTGATGG+AGG | 0.630279 | 4_2:+66226069 | None:intergenic |
| TTAGTATAGATAGAAGTGTG+TGG | 0.638820 | 4_2:+66228315 | None:intergenic |
| CTGCACAACCATGTAGGACA+AGG | 0.642790 | 4_2:+66226250 | None:intergenic |
| ACAGAGTCATAGGCTTGGCA+GGG | 0.644912 | 4_2:+66226367 | None:intergenic |
| GGCAGCGGCAGACATCGTCA+TGG | 0.658799 | 4_2:-66226000 | Msa0616430:CDS |
| ACATGAATAACAAAATACCT+GGG | 0.660009 | 4_2:+66225732 | None:intergenic |
| ACAACCATGTAGGACAAGGT+CGG | 0.664828 | 4_2:+66226254 | None:intergenic |
| AAAATGAAACAGCTTCTGCG+TGG | 0.667909 | 4_2:+66225950 | None:intergenic |
| GAGAAAGGCAGAGACAAAGG+AGG | 0.668200 | 4_2:+66228475 | None:intergenic |
| GTTACACGGACAAAATCGAT+GGG | 0.671108 | 4_2:-66226399 | Msa0616430:CDS |
| TTCCTATTAGAGAAGTTACA+CGG | 0.672955 | 4_2:-66226413 | Msa0616430:CDS |
| TAGCACCAGCATTGGCAGAG+AGG | 0.673176 | 4_2:-66227319 | Msa0616430:CDS |
| AGGGTACACACCTTTGTTTG+GGG | 0.683056 | 4_2:-66228168 | Msa0616430:CDS |
| ACGTTATTATCCCCAAACAA+AGG | 0.684297 | 4_2:+66228158 | None:intergenic |
| ATAATTTATTGAAAACCCTG+AGG | 0.691845 | 4_2:+66225657 | None:intergenic |
| ACACGGACAAAATCGATGGG+AGG | 0.707346 | 4_2:-66226396 | Msa0616430:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTAAGAGACAAAAAT+AGG | + | chr4_2:66226274-66226293 | None:intergenic | 15.0% |
| !! | AAAAATCCAATAAAAGTAAA+AGG | - | chr4_2:66226586-66226605 | Msa0616430:intron | 15.0% |
| !!! | ATTATTCCTTTTACTTTTAT+TGG | + | chr4_2:66226595-66226614 | None:intergenic | 15.0% |
| !! | AAAAAAAAATATTGCATGAA+AGG | + | chr4_2:66226981-66227000 | None:intergenic | 15.0% |
| !! | ATCTAGAAATTTACCTATTA+CGG | - | chr4_2:66225870-66225889 | Msa0616430:three_prime_UTR | 20.0% |
| !!! | GGATAAAGTATTAATCTTTT+TGG | - | chr4_2:66226041-66226060 | Msa0616430:CDS | 20.0% |
| !!! | TGAAATAGATTTTGAGTTTT+TGG | - | chr4_2:66226677-66226696 | Msa0616430:intron | 20.0% |
| !!! | GAAATAGATTTTGAGTTTTT+GGG | - | chr4_2:66226678-66226697 | Msa0616430:intron | 20.0% |
| !! | TAGTATTACTAAGAAAATGT+AGG | + | chr4_2:66226931-66226950 | None:intergenic | 20.0% |
| !!! | CAATAAAGATCTTTGTTTAT+AGG | - | chr4_2:66227021-66227040 | Msa0616430:intron | 20.0% |
| !!! | CGAATATGATAATTTTGATA+TGG | - | chr4_2:66228268-66228287 | Msa0616430:CDS | 20.0% |
| !! | AACGAAAAAGCATAGAAAAT+AGG | + | chr4_2:66225837-66225856 | None:intergenic | 25.0% |
| !!! | TTTTTGGTTTTTCTGTTGTT+GGG | - | chr4_2:66226057-66226076 | Msa0616430:CDS | 25.0% |
| ! | ACTCAAAATCTATTTCATCA+TGG | + | chr4_2:66226674-66226693 | None:intergenic | 25.0% |
| !! | TTTGGAATGTAAAAATCCAA+AGG | + | chr4_2:66226901-66226920 | None:intergenic | 25.0% |
| ! | GAAAATGTAGGTATATTCTT+TGG | + | chr4_2:66226919-66226938 | None:intergenic | 25.0% |
| ! | AAAGATCTTTATTGCATGAA+AGG | + | chr4_2:66227015-66227034 | None:intergenic | 25.0% |
| !! | AAGGTATCTATATATGACTT+GGG | + | chr4_2:66227128-66227147 | None:intergenic | 25.0% |
| ! | TTTAGTTGTTTATCCAAACA+GGG | + | chr4_2:66227179-66227198 | None:intergenic | 25.0% |
| ! | ATTTAGTTGTTTATCCAAAC+AGG | + | chr4_2:66227180-66227199 | None:intergenic | 25.0% |
| !! | AATAAGTTGAAATCAGCTTA+TGG | - | chr4_2:66227331-66227350 | Msa0616430:CDS | 25.0% |
| ! | AGATGCAAGTAAATATTTGT+CGG | + | chr4_2:66227396-66227415 | None:intergenic | 25.0% |
| ! | TCAAAACATCGTAATTAACA+TGG | + | chr4_2:66227564-66227583 | None:intergenic | 25.0% |
| ! | GAAAACAGGAATACAATATT+AGG | + | chr4_2:66227611-66227630 | None:intergenic | 25.0% |
| ! | ACCAGTACAAAAAATCAAAA+TGG | - | chr4_2:66227914-66227933 | Msa0616430:CDS | 25.0% |
| !!! | TCCATTTTGATTTTTTGTAC+TGG | + | chr4_2:66227918-66227937 | None:intergenic | 25.0% |
| ! | GATTAATTTCATCCTTGATT+CGG | - | chr4_2:66228162-66228181 | Msa0616430:CDS | 25.0% |
| !!! | AATTTTGATATGGTTCCTTT+TGG | - | chr4_2:66228278-66228297 | Msa0616430:exon | 25.0% |
| ! | ACATGAATAACAAAATACCT+GGG | + | chr4_2:66228342-66228361 | None:intergenic | 25.0% |
| ! | ATAATTTATTGAAAACCCTG+AGG | + | chr4_2:66228417-66228436 | None:intergenic | 25.0% |
| ! | CATGAGTTATTTAATGAAGT+AGG | - | chr4_2:66228441-66228460 | Msa0616430:five_prime_UTR | 25.0% |
| AAAGATAGATAGAAAGAGAG+AGG | + | chr4_2:66225683-66225702 | None:intergenic | 30.0% | |
| AAGAAGTGATGCAAAAAACA+GGG | + | chr4_2:66225727-66225746 | None:intergenic | 30.0% | |
| AAAGAAGTGATGCAAAAAAC+AGG | + | chr4_2:66225728-66225747 | None:intergenic | 30.0% | |
| ! | TTAGTATAGATAGAAGTGTG+TGG | + | chr4_2:66225759-66225778 | None:intergenic | 30.0% |
| ! | ATTCATGGGTTATTCTCATA+TGG | - | chr4_2:66225801-66225820 | Msa0616430:three_prime_UTR | 30.0% |
| CTTTCTTTAGATGAAAGAAC+AGG | - | chr4_2:66225967-66225986 | Msa0616430:CDS | 30.0% | |
| TTGATGTTACTATGATCTTG+TGG | - | chr4_2:66226020-66226039 | Msa0616430:CDS | 30.0% | |
| !!! | CTTTTTGGTTTTTCTGTTGT+TGG | - | chr4_2:66226056-66226075 | Msa0616430:CDS | 30.0% |
| TCTCATGATATTTACCTTCA+TGG | - | chr4_2:66226106-66226125 | Msa0616430:CDS | 30.0% | |
| CTCATGATATTTACCTTCAT+GGG | - | chr4_2:66226107-66226126 | Msa0616430:CDS | 30.0% | |
| ATAAAAAGCAACAACAACAC+CGG | + | chr4_2:66226173-66226192 | None:intergenic | 30.0% | |
| CTTGAGAATATAATCCGATA+AGG | - | chr4_2:66226317-66226336 | Msa0616430:CDS | 30.0% | |
| TATTCAGCTGTTGAAATTCA+AGG | - | chr4_2:66226360-66226379 | Msa0616430:CDS | 30.0% | |
| ACTGATTGTGACTAAAACTT+AGG | - | chr4_2:66226474-66226493 | Msa0616430:intron | 30.0% | |
| ! | CAAGGTATCTATATATGACT+TGG | + | chr4_2:66227129-66227148 | None:intergenic | 30.0% |
| GATGCAAGTAAATATTTGTC+GGG | + | chr4_2:66227395-66227414 | None:intergenic | 30.0% | |
| TGAAGATGCTTAAAATCTGT+CGG | - | chr4_2:66227428-66227447 | Msa0616430:CDS | 30.0% | |
| GGCTTATAATCAAATCATCT+TGG | - | chr4_2:66227518-66227537 | Msa0616430:intron | 30.0% | |
| TCTACATAAAATCTGCACAA+GGG | + | chr4_2:66227633-66227652 | None:intergenic | 30.0% | |
| ATCTACATAAAATCTGCACA+AGG | + | chr4_2:66227634-66227653 | None:intergenic | 30.0% | |
| TTCCTATTAGAGAAGTTACA+CGG | - | chr4_2:66227658-66227677 | Msa0616430:intron | 30.0% | |
| AAATCAAAATGGAGAACTTC+AGG | - | chr4_2:66227925-66227944 | Msa0616430:CDS | 30.0% | |
| !! | GAAGCTGTTTCATTTTAAGA+AGG | - | chr4_2:66228128-66228147 | Msa0616430:CDS | 30.0% |
| AAAAGAAAAATTAACCCAGG+TGG | + | chr4_2:66228196-66228215 | None:intergenic | 30.0% | |
| AGGAAAAGAAAAATTAACCC+AGG | + | chr4_2:66228199-66228218 | None:intergenic | 30.0% | |
| ! | TTTTTCTTTTCCTTTCTTCG+TGG | - | chr4_2:66228206-66228225 | Msa0616430:CDS | 30.0% |
| TATAGAGAGAGAAAGAAAGA+GGG | - | chr4_2:66228231-66228250 | Msa0616430:CDS | 30.0% | |
| ATGTGTATAGTTCATACCTT+AGG | - | chr4_2:66228314-66228333 | Msa0616430:five_prime_UTR | 30.0% | |
| GACATGAATAACAAAATACC+TGG | + | chr4_2:66228343-66228362 | None:intergenic | 30.0% | |
| TATTTAGACCATATACCTCA+GGG | - | chr4_2:66228399-66228418 | Msa0616430:five_prime_UTR | 30.0% | |
| TAGATAGAAAGAGAGAGGTT+TGG | + | chr4_2:66225678-66225697 | None:intergenic | 35.0% | |
| ! | ATTTCTAGATGAAGCAGAAG+CGG | + | chr4_2:66225860-66225879 | None:intergenic | 35.0% |
| ACGTTATTATCCCCAAACAA+AGG | + | chr4_2:66225916-66225935 | None:intergenic | 35.0% | |
| AACGTCTTTGTTCATAGAGA+TGG | - | chr4_2:66225931-66225950 | Msa0616430:exon | 35.0% | |
| TGTTCATAGAGATGGCAAAT+CGG | - | chr4_2:66225939-66225958 | Msa0616430:exon | 35.0% | |
| AACAGGTTTGAAAATCTGCA+TGG | - | chr4_2:66225984-66226003 | Msa0616430:CDS | 35.0% | |
| ACAGGTTTGAAAATCTGCAT+GGG | - | chr4_2:66225985-66226004 | Msa0616430:CDS | 35.0% | |
| AATTTCAACAGTGACGTTTC+TGG | + | chr4_2:66226539-66226558 | None:intergenic | 35.0% | |
| TTGTATCTGCAGATGTCAAA+TGG | - | chr4_2:66226630-66226649 | Msa0616430:intron | 35.0% | |
| !! | GAGTTTTTGGGAAACATTAG+AGG | - | chr4_2:66226690-66226709 | Msa0616430:intron | 35.0% |
| GAAACATTAGAGGCAAAGAT+TGG | - | chr4_2:66226700-66226719 | Msa0616430:intron | 35.0% | |
| AGGATTCAGACCAATGTTTA+TGG | - | chr4_2:66226723-66226742 | Msa0616430:intron | 35.0% | |
| TCCATCAATACAGTATTCTC+TGG | - | chr4_2:66226811-66226830 | Msa0616430:intron | 35.0% | |
| TCCAGAGAATACTGTATTGA+TGG | + | chr4_2:66226815-66226834 | None:intergenic | 35.0% | |
| AACCTCATACATACCATATC+TGG | - | chr4_2:66226871-66226890 | Msa0616430:intron | 35.0% | |
| AACCAGATATGGTATGTATG+AGG | + | chr4_2:66226876-66226895 | None:intergenic | 35.0% | |
| !!! | ATCTAGCTGTTTTTTGCTGT+TGG | - | chr4_2:66227054-66227073 | Msa0616430:intron | 35.0% |
| !!! | TCTAGCTGTTTTTTGCTGTT+GGG | - | chr4_2:66227055-66227074 | Msa0616430:intron | 35.0% |
| AAGCAACTTATGAAAACAGC+TGG | + | chr4_2:66227306-66227325 | None:intergenic | 35.0% | |
| TAAATATTTGTCGGGCTATC+TGG | + | chr4_2:66227387-66227406 | None:intergenic | 35.0% | |
| CAACTGATCAAACATTCACA+CGG | + | chr4_2:66227468-66227487 | None:intergenic | 35.0% | |
| TATATGGGATGCATCAGATT+GGG | - | chr4_2:66227734-66227753 | Msa0616430:intron | 35.0% | |
| CTTACTGTTACGACAAAATC+AGG | - | chr4_2:66227967-66227986 | Msa0616430:CDS | 35.0% | |
| GGATGAAATTAATCTTCGTG+TGG | + | chr4_2:66228156-66228175 | None:intergenic | 35.0% | |
| GTATAGAGAGAGAAAGAAAG+AGG | - | chr4_2:66228230-66228249 | Msa0616430:CDS | 35.0% | |
| ATAGAGAGAGAAAGAAAGAG+GGG | - | chr4_2:66228232-66228251 | Msa0616430:CDS | 35.0% | |
| ! | TTTGATATGGTTCCTTTTGG+TGG | - | chr4_2:66228281-66228300 | Msa0616430:exon | 35.0% |
| GAGGTATATGGTCTAAATAC+AGG | + | chr4_2:66228398-66228417 | None:intergenic | 35.0% | |
| GTATTTAGACCATATACCTC+AGG | - | chr4_2:66228398-66228417 | Msa0616430:five_prime_UTR | 35.0% | |
| ATTGAAAACCCTGAGGTATA+TGG | + | chr4_2:66228410-66228429 | None:intergenic | 35.0% | |
| CTGGTTATTAGAGAGGAGAA+AGG | + | chr4_2:66225614-66225633 | None:intergenic | 40.0% | |
| AAACAGGGTAACGAAGAAGA+AGG | + | chr4_2:66225712-66225731 | None:intergenic | 40.0% | |
| CTATTACGGCCTTTGATGAA+GGG | - | chr4_2:66225884-66225903 | Msa0616430:three_prime_UTR | 40.0% | |
| ! | AAGGGTACACACCTTTGTTT+GGG | - | chr4_2:66225902-66225921 | Msa0616430:three_prime_UTR | 40.0% |
| !! | AAGCACTGAAATACCCATGA+AGG | + | chr4_2:66226123-66226142 | None:intergenic | 40.0% |
| ACATTAGAGGCAAAGATTGG+AGG | - | chr4_2:66226703-66226722 | Msa0616430:intron | 40.0% | |
| CAGACCAATGTTTATGGCAA+TGG | - | chr4_2:66226729-66226748 | Msa0616430:intron | 40.0% | |
| GCTACCATTGCCATAAACAT+TGG | + | chr4_2:66226736-66226755 | None:intergenic | 40.0% | |
| ATTGGCAGAGAGGAAAGATA+TGG | - | chr4_2:66226762-66226781 | Msa0616430:intron | 40.0% | |
| TATTGATGGAAATCCTCTGC+AGG | + | chr4_2:66226801-66226820 | None:intergenic | 40.0% | |
| ! | TACCATATCTGGTTCACCTT+TGG | - | chr4_2:66226882-66226901 | Msa0616430:intron | 40.0% |
| ATCCAAAGGTGAACCAGATA+TGG | + | chr4_2:66226887-66226906 | None:intergenic | 40.0% | |
| !!! | GTTTTTTGCTGTTGGGTTTG+TGG | - | chr4_2:66227062-66227081 | Msa0616430:intron | 40.0% |
| ATGTTTGATCAGTTGACAGC+AGG | - | chr4_2:66227473-66227492 | Msa0616430:intron | 40.0% | |
| TGTTTGATCAGTTGACAGCA+GGG | - | chr4_2:66227474-66227493 | Msa0616430:intron | 40.0% | |
| ATTTGATTATAAGCCCCCGA+TGG | + | chr4_2:66227513-66227532 | None:intergenic | 40.0% | |
| AAATCTGCACAAGGGAAAAC+AGG | + | chr4_2:66227625-66227644 | None:intergenic | 40.0% | |
| GTCCGTGTAACTTCTCTAAT+AGG | + | chr4_2:66227663-66227682 | None:intergenic | 40.0% | |
| AGTTACACGGACAAAATCGA+TGG | - | chr4_2:66227671-66227690 | Msa0616430:intron | 40.0% | |
| GTTACACGGACAAAATCGAT+GGG | - | chr4_2:66227672-66227691 | Msa0616430:intron | 40.0% | |
| ATAGTCGCATACAGAGTCAT+AGG | + | chr4_2:66227717-66227736 | None:intergenic | 40.0% | |
| TGACTCTGTATGCGACTATA+TGG | - | chr4_2:66227718-66227737 | Msa0616430:intron | 40.0% | |
| GACTCTGTATGCGACTATAT+GGG | - | chr4_2:66227719-66227738 | Msa0616430:intron | 40.0% | |
| CTATATGGGATGCATCAGAT+TGG | - | chr4_2:66227733-66227752 | Msa0616430:intron | 40.0% | |
| TCGGAGAATTTGGCAACATA+TGG | + | chr4_2:66227801-66227820 | None:intergenic | 40.0% | |
| AGTTCCAAATCCATTCCTTC+TGG | - | chr4_2:66227885-66227904 | Msa0616430:intron | 40.0% | |
| ! | AACACCAGAAGGAATGGATT+TGG | + | chr4_2:66227892-66227911 | None:intergenic | 40.0% |
| TCAATCCACAAGAAGCTGAA+AGG | - | chr4_2:66228021-66228040 | Msa0616430:intron | 40.0% | |
| AAATTCGACCCTGTCACATT+CGG | - | chr4_2:66228050-66228069 | Msa0616430:intron | 40.0% | |
| AAAATGAAACAGCTTCTGCG+TGG | + | chr4_2:66228124-66228143 | None:intergenic | 40.0% | |
| TCTCTATACACCACGAAGAA+AGG | + | chr4_2:66228219-66228238 | None:intergenic | 40.0% | |
| !! | ATATGGTTCCTTTTGGTGGT+GGG | - | chr4_2:66228285-66228304 | Msa0616430:exon | 40.0% |
| ACAAAATACCTGGGAACCTA+AGG | + | chr4_2:66228333-66228352 | None:intergenic | 40.0% | |
| GTGCTAGCTGGTTATTAGAG+AGG | + | chr4_2:66225621-66225640 | None:intergenic | 45.0% | |
| AACAGCTAGAAGCGATAGAG+AGG | + | chr4_2:66225643-66225662 | None:intergenic | 45.0% | |
| CCTTCATCAAAGGCCGTAAT+AGG | + | chr4_2:66225886-66225905 | None:intergenic | 45.0% | |
| CCTATTACGGCCTTTGATGA+AGG | - | chr4_2:66225883-66225902 | Msa0616430:three_prime_UTR | 45.0% | |
| AGGTGTGTACCCTTCATCAA+AGG | + | chr4_2:66225896-66225915 | None:intergenic | 45.0% | |
| ! | GAAGGGTACACACCTTTGTT+TGG | - | chr4_2:66225901-66225920 | Msa0616430:three_prime_UTR | 45.0% |
| ! | AGGGTACACACCTTTGTTTG+GGG | - | chr4_2:66225903-66225922 | Msa0616430:three_prime_UTR | 45.0% |
| ! | TGTTGTTGGGTTGTGTTTGC+AGG | - | chr4_2:66226070-66226089 | Msa0616430:CDS | 45.0% |
| ! | TTACCTGCTGATTACACTGC+CGG | - | chr4_2:66226151-66226170 | Msa0616430:CDS | 45.0% |
| AGTCACAATCAGTGAGCATG+CGG | + | chr4_2:66226467-66226486 | None:intergenic | 45.0% | |
| ! | TTCAACAGTGACGTTTCTGG+TGG | + | chr4_2:66226536-66226555 | None:intergenic | 45.0% |
| GAGAGGAAAGATATGGTCTC+TGG | - | chr4_2:66226769-66226788 | Msa0616430:intron | 45.0% | |
| AACAGGGCAAATCGAATGTG+TGG | + | chr4_2:66227163-66227182 | None:intergenic | 45.0% | |
| ! | CACATTCGATTTGCCCTGTT+TGG | - | chr4_2:66227163-66227182 | Msa0616430:intron | 45.0% |
| GGGAATCCATATCCTACCAT+CGG | - | chr4_2:66227494-66227513 | Msa0616430:intron | 45.0% | |
| GGAATCCATATCCTACCATC+GGG | - | chr4_2:66227495-66227514 | Msa0616430:intron | 45.0% | |
| GAATCCATATCCTACCATCG+GGG | - | chr4_2:66227496-66227515 | Msa0616430:intron | 45.0% | |
| AATCCATATCCTACCATCGG+GGG | - | chr4_2:66227497-66227516 | Msa0616430:intron | 45.0% | |
| CATCAGATTGGGCTACCAAT+GGG | - | chr4_2:66227745-66227764 | Msa0616430:intron | 45.0% | |
| ATCAGATTGGGCTACCAATG+GGG | - | chr4_2:66227746-66227765 | Msa0616430:intron | 45.0% | |
| AACTCTGTACTTTCCCCCAT+TGG | + | chr4_2:66227763-66227782 | None:intergenic | 45.0% | |
| TAGGACAAGGTCGGAGAATT+TGG | + | chr4_2:66227811-66227830 | None:intergenic | 45.0% | |
| ACAACCATGTAGGACAAGGT+CGG | + | chr4_2:66227820-66227839 | None:intergenic | 45.0% | |
| ACTTTGAGCAGTGTCACACT+TGG | + | chr4_2:66227868-66227887 | None:intergenic | 45.0% | |
| TGGTGTAACACCAGAAGGAA+TGG | + | chr4_2:66227898-66227917 | None:intergenic | 45.0% | |
| ! | TGTACTGGTGTAACACCAGA+AGG | + | chr4_2:66227903-66227922 | None:intergenic | 45.0% |
| ! | CTTAGCCTTTCAGCTTCTTG+TGG | + | chr4_2:66228029-66228048 | None:intergenic | 45.0% |
| CACAAGAAGCTGAAAGGCTA+AGG | - | chr4_2:66228027-66228046 | Msa0616430:intron | 45.0% | |
| GTGGTAATCGAACCGAATCA+AGG | + | chr4_2:66228177-66228196 | None:intergenic | 45.0% | |
| ! | ATTCGGTTCGATTACCACCT+GGG | - | chr4_2:66228179-66228198 | Msa0616430:CDS | 45.0% |
| ! | GATATGGTTCCTTTTGGTGG+TGG | - | chr4_2:66228284-66228303 | Msa0616430:exon | 45.0% |
| !!! | TATGGTTCCTTTTGGTGGTG+GGG | - | chr4_2:66228286-66228305 | Msa0616430:exon | 45.0% |
| AGTTCATACCTTAGGTTCCC+AGG | - | chr4_2:66228322-66228341 | Msa0616430:five_prime_UTR | 45.0% | |
| !! | TAAAAAAAAAATATAATTCA+TGG | - | chr4_2:66225786-66225805 | Msa0616430:three_prime_UTR | 5.0% |
| !! | AAAAAAAAAATATAATTCAT+GGG | - | chr4_2:66225787-66225806 | Msa0616430:three_prime_UTR | 5.0% |
| GAGAAAGGCAGAGACAAAGG+AGG | + | chr4_2:66225599-66225618 | None:intergenic | 50.0% | |
| GAGGAGAAAGGCAGAGACAA+AGG | + | chr4_2:66225602-66225621 | None:intergenic | 50.0% | |
| TGGGTTGTGTTTGCAGGTTC+TGG | - | chr4_2:66226076-66226095 | Msa0616430:CDS | 50.0% | |
| TCCGATAAGGTCTCAGTCTC+AGG | - | chr4_2:66226330-66226349 | Msa0616430:CDS | 50.0% | |
| CCCTGAGACTGAGACCTTAT+CGG | + | chr4_2:66226334-66226353 | None:intergenic | 50.0% | |
| CCGATAAGGTCTCAGTCTCA+GGG | - | chr4_2:66226331-66226350 | Msa0616430:CDS | 50.0% | |
| AATCAGTGAGCATGCGGTTG+AGG | + | chr4_2:66226461-66226480 | None:intergenic | 50.0% | |
| TCTTTCCTCTCTGCCAATGC+TGG | + | chr4_2:66226760-66226779 | None:intergenic | 50.0% | |
| ! | TCTCTGGTTTGATCCTGCAG+AGG | - | chr4_2:66226785-66226804 | Msa0616430:intron | 50.0% |
| TGTGTGGTCTCTTGACAGCA+AGG | + | chr4_2:66227147-66227166 | None:intergenic | 50.0% | |
| GATTATAAGCCCCCGATGGT+AGG | + | chr4_2:66227509-66227528 | None:intergenic | 50.0% | |
| ACACGGACAAAATCGATGGG+AGG | - | chr4_2:66227675-66227694 | Msa0616430:intron | 50.0% | |
| ACAGAGTCATAGGCTTGGCA+GGG | + | chr4_2:66227707-66227726 | None:intergenic | 50.0% | |
| TACAGAGTCATAGGCTTGGC+AGG | + | chr4_2:66227708-66227727 | None:intergenic | 50.0% | |
| CGCATACAGAGTCATAGGCT+TGG | + | chr4_2:66227712-66227731 | None:intergenic | 50.0% | |
| GCATCAGATTGGGCTACCAA+TGG | - | chr4_2:66227744-66227763 | Msa0616430:intron | 50.0% | |
| TCAGATTGGGCTACCAATGG+GGG | - | chr4_2:66227747-66227766 | Msa0616430:intron | 50.0% | |
| TTCTCCGACCTTGTCCTACA+TGG | - | chr4_2:66227813-66227832 | Msa0616430:intron | 50.0% | |
| CTGCACAACCATGTAGGACA+AGG | + | chr4_2:66227824-66227843 | None:intergenic | 50.0% | |
| GATCGACTGCACAACCATGT+AGG | + | chr4_2:66227830-66227849 | None:intergenic | 50.0% | |
| CACTTGGCTACATGCTCGAT+TGG | + | chr4_2:66227852-66227871 | None:intergenic | 50.0% | |
| TTGATCACGCACTCTGATGG+AGG | + | chr4_2:66228005-66228024 | None:intergenic | 50.0% | |
| GGATTGATCACGCACTCTGA+TGG | + | chr4_2:66228008-66228027 | None:intergenic | 50.0% | |
| ! | GATTCGGTTCGATTACCACC+TGG | - | chr4_2:66228178-66228197 | Msa0616430:CDS | 50.0% |
| !!! | ATGGTTCCTTTTGGTGGTGG+GGG | - | chr4_2:66228287-66228306 | Msa0616430:exon | 50.0% |
| !! | AGCGATAGAGAGGTGCTAGC+TGG | + | chr4_2:66225633-66225652 | None:intergenic | 55.0% |
| ACACCGGCAGTGTAATCAGC+AGG | + | chr4_2:66226157-66226176 | None:intergenic | 55.0% | |
| GGCAATGGTAGCACCAGCAT+TGG | - | chr4_2:66226744-66226763 | Msa0616430:intron | 55.0% | |
| !! | TAGCACCAGCATTGGCAGAG+AGG | - | chr4_2:66226752-66226771 | Msa0616430:intron | 55.0% |
| AAGCCCCCGATGGTAGGATA+TGG | + | chr4_2:66227503-66227522 | None:intergenic | 55.0% | |
| AAACGACACCACCACAGCAG+AGG | - | chr4_2:66228095-66228114 | Msa0616430:intron | 55.0% | |
| !!! | TGGTTCCTTTTGGTGGTGGG+GGG | - | chr4_2:66228288-66228307 | Msa0616430:exon | 55.0% |
| GACCCTGTCACATTCGGCAG+CGG | - | chr4_2:66228056-66228075 | Msa0616430:intron | 60.0% | |
| TGCCGCTGCCGAATGTGACA+GGG | + | chr4_2:66228061-66228080 | None:intergenic | 60.0% | |
| CGTGGTTTCCTCTGCTGTGG+TGG | + | chr4_2:66228106-66228125 | None:intergenic | 60.0% | |
| CTGCGTGGTTTCCTCTGCTG+TGG | + | chr4_2:66228109-66228128 | None:intergenic | 60.0% | |
| !!! | GGTTCCTTTTGGTGGTGGGG+GGG | - | chr4_2:66228289-66228308 | Msa0616430:five_prime_UTR | 60.0% |
| !!! | GTTCCTTTTGGTGGTGGGGG+GGG | - | chr4_2:66228290-66228309 | Msa0616430:five_prime_UTR | 60.0% |
| ATACCCCCCCCACCACCAAA+AGG | + | chr4_2:66228296-66228315 | None:intergenic | 60.0% | |
| CTGCCGCTGCCGAATGTGAC+AGG | + | chr4_2:66228062-66228081 | None:intergenic | 65.0% | |
| GGCAGCGGCAGACATCGTCA+TGG | - | chr4_2:66228071-66228090 | Msa0616430:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 66225572 | 66228521 | 66225572 | ID=Msa0616430;Name=Msa0616430 |
| chr4_2 | mRNA | 66225572 | 66228521 | 66225572 | ID=Msa0616430-mRNA-1;Parent=Msa0616430;Name=Msa0616430-mRNA-1;_AED=0.12;_eAED=0.12;_QI=233|1|1|1|1|1|4|376|332 |
| chr4_2 | exon | 66225572 | 66226452 | 66225572 | ID=Msa0616430-mRNA-1:exon:18855;Parent=Msa0616430-mRNA-1 |
| chr4_2 | exon | 66227243 | 66227451 | 66227243 | ID=Msa0616430-mRNA-1:exon:18854;Parent=Msa0616430-mRNA-1 |
| chr4_2 | exon | 66227901 | 66228001 | 66227901 | ID=Msa0616430-mRNA-1:exon:18853;Parent=Msa0616430-mRNA-1 |
| chr4_2 | exon | 66228105 | 66228521 | 66228105 | ID=Msa0616430-mRNA-1:exon:18852;Parent=Msa0616430-mRNA-1 |
| chr4_2 | five_prime_UTR | 66228289 | 66228521 | 66228289 | ID=Msa0616430-mRNA-1:five_prime_utr;Parent=Msa0616430-mRNA-1 |
| chr4_2 | CDS | 66228105 | 66228288 | 66228105 | ID=Msa0616430-mRNA-1:cds;Parent=Msa0616430-mRNA-1 |
| chr4_2 | CDS | 66227901 | 66228001 | 66227901 | ID=Msa0616430-mRNA-1:cds;Parent=Msa0616430-mRNA-1 |
| chr4_2 | CDS | 66227243 | 66227451 | 66227243 | ID=Msa0616430-mRNA-1:cds;Parent=Msa0616430-mRNA-1 |
| chr4_2 | CDS | 66225948 | 66226452 | 66225948 | ID=Msa0616430-mRNA-1:cds;Parent=Msa0616430-mRNA-1 |
| chr4_2 | three_prime_UTR | 66225572 | 66225947 | 66225572 | ID=Msa0616430-mRNA-1:three_prime_utr;Parent=Msa0616430-mRNA-1 |
| Gene Sequence |
| Protein sequence |
