AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0618710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0618710 MtrunA17_Chr8g0379901 98.188 276 5 0 1 276 1 276 0.0 550
Msa0618710 MtrunA17_Chr8g0379911 73.585 265 70 0 4 268 3 267 6.03e-151 422
Msa0618710 MtrunA17_Chr2g0284581 71.218 271 78 0 4 274 3 273 2.85e-144 405
Msa0618710 MtrunA17_Chr2g0284591 68.340 259 82 0 13 271 10 268 3.62e-135 382
Msa0618710 MtrunA17_Chr2g0284631 64.706 272 89 2 4 268 55 326 1.69e-130 373
Msa0618710 MtrunA17_Chr2g0284611 65.789 266 91 0 4 269 3 268 1.47e-128 365
Msa0618710 MtrunA17_Chr2g0284601 74.545 220 56 0 55 274 6 225 1.14e-122 349
Msa0618710 MtrunA17_Chr4g0044201 51.292 271 113 3 9 273 1 258 4.43e-97 285
Msa0618710 MtrunA17_Chr5g0435051 50.775 258 119 4 1 254 1 254 2.10e-85 256
Msa0618710 MtrunA17_Chr3g0079421 53.556 239 108 2 19 257 8 243 2.59e-83 251
Msa0618710 MtrunA17_Chr4g0001221 53.680 231 102 2 35 263 71 298 5.17e-78 238
Msa0618710 MtrunA17_Chr2g0333771 52.863 227 102 2 39 263 80 303 3.50e-76 234
Msa0618710 MtrunA17_Chr7g0217331 48.148 216 111 1 37 252 35 249 9.77e-73 224
Msa0618710 MtrunA17_Chr1g0201331 49.362 235 108 3 34 260 69 300 1.94e-69 216
Msa0618710 MtrunA17_Chr5g0426131 46.442 267 117 8 4 261 10 259 1.06e-66 208
Msa0618710 MtrunA17_Chr1g0184541 40.271 221 131 1 33 253 56 275 4.06e-49 164
Msa0618710 MtrunA17_Chr4g0001111 52.000 150 67 2 35 182 71 217 5.40e-40 139
Msa0618710 MtrunA17_Chr4g0001161 49.645 141 66 2 52 190 1 138 3.11e-33 118
Msa0618710 MtrunA17_Chr4g0001121 56.098 82 36 0 182 263 10 91 1.96e-28 104
Msa0618710 MtrunA17_Chr8g0340161 33.028 218 139 3 38 248 18 235 1.14e-26 104
Msa0618710 MtrunA17_Chr2g0331261 32.645 242 145 7 34 264 15 249 2.33e-25 100
Msa0618710 MtrunA17_Chr5g0399341 33.047 233 139 5 38 257 18 246 8.11e-25 99.4
Msa0618710 MtrunA17_Chr4g0001151 45.865 133 52 3 52 182 1 115 1.22e-23 97.8
Msa0618710 MtrunA17_Chr1g0146161 31.120 241 145 8 13 246 3 229 1.85e-23 95.9
Msa0618710 MtrunA17_Chr1g0188771 32.444 225 143 5 38 254 20 243 2.33e-23 95.9
Msa0618710 MtrunA17_Chr6g0478191 32.159 227 141 5 38 254 20 243 6.24e-23 94.7
Msa0618710 MtrunA17_Chr1g0146171 31.102 254 148 11 13 255 3 240 1.01e-22 94.0
Msa0618710 MtrunA17_Chr3g0145671 31.222 221 137 5 37 242 35 255 1.19e-22 94.4
Msa0618710 MtrunA17_Chr7g0264961 33.180 217 135 6 38 246 20 234 3.38e-21 89.7
Msa0618710 MtrunA17_Chr1g0212671 32.759 232 142 7 38 257 20 249 5.73e-21 89.4
Msa0618710 MtrunA17_Chr2g0326621 30.837 227 133 6 39 242 40 265 1.14e-20 89.4
Msa0618710 MtrunA17_Chr7g0264111 30.357 224 133 6 37 238 37 259 3.42e-20 87.8
Msa0618710 MtrunA17_Chr4g0030861 32.287 223 142 5 44 258 26 247 4.06e-20 87.0
Msa0618710 MtrunA17_Chr4g0006001 28.409 264 164 8 1 248 26 280 8.20e-20 87.0
Msa0618710 MtrunA17_Chr1g0199731 28.390 236 142 5 37 248 37 269 9.46e-20 86.7
Msa0618710 MtrunA17_Chr8g0386961 28.369 282 173 9 2 259 11 287 1.56e-19 85.9
Msa0618710 MtrunA17_Chr4g0029071 30.568 229 134 7 37 242 37 263 7.81e-19 84.0
Msa0618710 MtrunA17_Chr5g0397391 29.289 239 145 7 30 248 43 277 8.97e-19 84.0
Msa0618710 MtrunA17_Chr5g0436701 29.707 239 145 7 37 256 53 287 9.88e-19 84.0
Msa0618710 MtrunA17_Chr3g0113211 26.236 263 177 6 10 256 26 287 1.84e-18 83.2
Msa0618710 MtrunA17_Chr4g0020781 31.492 181 121 2 75 253 20 199 1.94e-16 76.3
Msa0618710 MtrunA17_Chr7g0222791 27.706 231 160 3 38 261 20 250 1.82e-14 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0618710 AT4G18910.1 71.486 249 64 1 33 274 46 294 3.17e-131 374
Msa0618710 AT4G19030.1 68.045 266 78 1 8 266 24 289 1.59e-128 367
Msa0618710 AT5G37820.1 60.000 235 94 0 39 273 43 277 2.40e-101 297
Msa0618710 AT5G37820.2 60.000 235 94 0 39 273 79 313 6.33e-101 298
Msa0618710 AT5G37810.1 53.333 270 119 2 3 266 2 270 6.26e-100 294
Msa0618710 AT1G31885.1 52.399 271 118 2 1 260 1 271 2.57e-98 291
Msa0618710 AT2G34390.1 53.101 258 113 2 17 267 26 282 7.44e-91 271
Msa0618710 AT4G18910.2 73.446 177 40 1 33 202 46 222 1.17e-89 265
Msa0618710 AT4G19030.3 65.672 201 62 1 8 201 24 224 6.52e-88 261
Msa0618710 AT1G31885.2 55.760 217 89 1 51 260 1 217 2.19e-86 259
Msa0618710 AT2G34390.2 53.782 238 102 2 37 267 4 240 2.62e-85 255
Msa0618710 AT4G19030.2 63.187 182 60 1 8 182 24 205 5.28e-76 231
Msa0618710 AT1G80760.1 50.820 244 113 3 12 253 55 293 3.20e-73 226
Msa0618710 AT4G10380.1 48.819 254 119 3 15 260 54 304 1.07e-72 225
Msa0618710 AT2G34390.3 55.959 193 77 2 17 202 26 217 1.45e-68 212
Msa0618710 AT2G34390.4 55.429 175 70 2 17 184 26 199 6.81e-62 195
Msa0618710 AT3G06100.1 38.057 247 150 3 10 254 16 261 7.80e-48 160
Msa0618710 AT3G06100.2 40.654 214 125 2 42 254 1 213 3.97e-47 157
Msa0618710 AT1G31885.3 48.936 141 65 2 1 134 1 141 1.60e-37 130
Msa0618710 AT3G16240.1 33.476 233 137 6 30 248 7 235 2.69e-29 112
Msa0618710 AT2G29870.1 52.688 93 44 0 154 246 20 112 7.43e-28 104
Msa0618710 AT5G47450.1 32.636 239 151 4 30 258 10 248 1.83e-27 107
Msa0618710 AT3G06100.3 38.298 141 85 2 115 254 41 180 3.04e-25 99.8
Msa0618710 AT4G17340.1 30.252 238 156 4 30 257 10 247 1.43e-24 99.4
Msa0618710 AT4G35100.1 31.579 228 141 5 30 242 29 256 2.39e-23 96.7
Msa0618710 AT4G35100.2 31.579 228 141 5 30 242 29 256 2.39e-23 96.7
Msa0618710 AT3G26520.1 33.486 218 138 3 38 248 21 238 7.48e-23 94.7
Msa0618710 AT1G17810.1 34.286 175 111 2 91 261 86 260 5.04e-22 92.8
Msa0618710 AT2G16850.1 30.769 221 138 5 37 242 34 254 5.12e-22 93.2
Msa0618710 AT2G36830.1 34.434 212 131 4 38 241 20 231 9.71e-22 91.7
Msa0618710 AT5G60660.1 29.362 235 143 6 36 248 36 269 6.44e-21 90.1
Msa0618710 AT2G39010.1 29.060 234 143 6 37 248 36 268 1.01e-20 89.7
Msa0618710 AT2G25810.1 32.420 219 135 7 38 248 18 231 2.86e-20 87.8
Msa0618710 AT1G73190.1 35.542 166 103 3 92 254 87 251 2.90e-20 88.2
Msa0618710 AT4G23400.1 28.629 248 155 6 30 259 44 287 6.85e-20 87.4
Msa0618710 AT2G37170.1 28.879 232 142 6 39 248 37 267 1.06e-19 87.0
Msa0618710 AT2G37170.2 28.879 232 142 6 39 248 80 310 1.25e-19 87.4
Msa0618710 AT3G61430.2 29.150 247 151 7 30 257 43 284 1.51e-19 86.3
Msa0618710 AT3G61430.1 29.150 247 151 7 30 257 43 284 1.51e-19 86.3
Msa0618710 AT4G01470.1 35.465 172 107 3 80 248 67 237 2.82e-19 85.1
Msa0618710 AT1G01620.1 29.412 238 145 5 30 248 43 276 3.00e-19 85.5
Msa0618710 AT3G54820.1 28.326 233 146 5 37 248 36 268 3.70e-19 85.5
Msa0618710 AT3G53420.1 28.755 233 145 6 37 248 37 269 4.36e-19 85.1
Msa0618710 AT3G53420.2 28.755 233 145 6 37 248 37 269 4.36e-19 85.1
Msa0618710 AT4G00430.1 27.509 269 170 6 2 248 12 277 4.54e-19 85.1
Msa0618710 AT2G45960.1 28.745 247 152 7 30 257 43 284 7.55e-19 84.3
Msa0618710 AT3G47440.1 29.224 219 145 4 38 248 22 238 2.87e-18 82.4
Msa0618710 AT2G37180.1 28.448 232 143 6 39 248 37 267 1.22e-17 81.3
Msa0618710 AT1G01620.2 31.720 186 107 4 69 238 13 194 1.46e-16 77.0
Msa0618710 AT2G45960.2 30.476 210 126 5 30 223 43 248 2.09e-16 77.4
Msa0618710 AT2G45960.3 30.476 210 126 5 30 223 43 248 2.16e-16 77.8
Msa0618710 AT2G39010.2 29.936 157 96 4 37 180 36 191 9.19e-12 63.5

Find 89 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTTATGTTTACCATTTC+TGG 0.053365 4_2:-69620772 Msa0618710:CDS
TACACTCTCTATATTTATTT+TGG 0.109717 4_2:+69619895 None:intergenic
TTCCAATTTCTTAAACTATT+TGG 0.141022 4_2:+69620199 None:intergenic
AACCAAATAGTTTAAGAAAT+TGG 0.160387 4_2:-69620201 Msa0618710:exon
ATATTTGCTGTCACCGATTT+TGG 0.177264 4_2:-69620331 Msa0618710:CDS
TCCTGGGATTGCAATTGTTT+GGG 0.194809 4_2:-69622175 Msa0618710:CDS
TCCTTGTTCTCTATCACTTA+TGG 0.247542 4_2:-69622521 Msa0618710:exon
AAATTGGAGTCGTTTATTTC+AGG 0.294484 4_2:-69622597 None:intergenic
GGTAGAAAGAAAACTAGTTT+TGG 0.295044 4_2:+69619932 None:intergenic
TTCCTGGGATTGCAATTGTT+TGG 0.304422 4_2:-69622176 Msa0618710:CDS
ATCAATTTAGCACCTTAATT+AGG 0.305497 4_2:+69620000 None:intergenic
CTGCATACACTTCCTAATTA+AGG 0.312351 4_2:-69620012 Msa0618710:three_prime_UTR
AAGGAGGTTTCCATTGTTAC+AGG 0.315994 4_2:-69622064 Msa0618710:intron
ACATATTTCTTGATATTTGC+AGG 0.316106 4_2:-69622246 Msa0618710:CDS
CTCTGTCCCTTTCTTGCAAA+AGG 0.317695 4_2:-69622404 Msa0618710:intron
TTCATTGATGCTCCTGTTAC+CGG 0.336316 4_2:+69620411 None:intergenic
TAATTAAGGTGCTAAATTGA+TGG 0.365761 4_2:-69619998 Msa0618710:three_prime_UTR
CAAGAAAGGGACAGAGATAA+AGG 0.377304 4_2:+69622411 None:intergenic
GACAACGTGGTAACACTTCC+TGG 0.380447 4_2:-69622192 Msa0618710:CDS
TTATTTGACAGGCCGGTAAC+AGG 0.383562 4_2:-69620423 Msa0618710:intron
TACTCACTTGGTCATATCTC+TGG 0.390477 4_2:-69622129 Msa0618710:CDS
TTGCTTAATGTGATGATTGC+AGG 0.400582 4_2:-69620595 Msa0618710:CDS
AATACAGTTAATAGCAGAAA+TGG 0.417887 4_2:-69622274 Msa0618710:intron
CTGCACATTAAAAGAAACTT+AGG 0.418065 4_2:+69619866 None:intergenic
GAGTTGGCTGGAATTGCAAT+TGG 0.418098 4_2:-69620628 Msa0618710:CDS
TCTGCATACATGATACTTGA+TGG 0.421277 4_2:+69620168 None:intergenic
TTGTAGATTGGTGAGTTGGC+TGG 0.424279 4_2:-69620640 Msa0618710:intron
GCTGGTCCTAGAGTTCTAAC+CGG 0.437239 4_2:+69620387 None:intergenic
TTATATATCAGCTCAGCTCT+TGG 0.447897 4_2:-69620917 Msa0618710:CDS
CATGGGTCTACAACACTGTT+AGG 0.451802 4_2:-69620293 Msa0618710:CDS
ATTCAAGAACAAAAGCTTGA+AGG 0.459020 4_2:+69620809 None:intergenic
AAGTGTATATATGATTATCA+AGG 0.459227 4_2:+69619770 None:intergenic
GCATCCTTTCTCAAAGAAGC+TGG 0.460217 4_2:-69620234 Msa0618710:CDS
CTCCAGAAATGGTAAACATA+AGG 0.466289 4_2:+69620770 None:intergenic
ATCGGTGGCAACTCCAGAAA+TGG 0.467754 4_2:+69620759 None:intergenic
ACGCCCAGCTTCTTTGAGAA+AGG 0.469829 4_2:+69620230 None:intergenic
CCTGGGATTGCAATTGTTTG+GGG 0.472067 4_2:-69622174 Msa0618710:CDS
TATATATCAGCTCAGCTCTT+GGG 0.473337 4_2:-69620916 Msa0618710:CDS
AGTTGGCTGGAATTGCAATT+GGG 0.481359 4_2:-69620627 Msa0618710:CDS
TTTGAGAAAGGATGCACTCT+GGG 0.482847 4_2:+69620242 None:intergenic
TGTTTGGGGACTAACTTTGT+TGG 0.484275 4_2:-69622160 Msa0618710:CDS
GTAGACCCATGCTCCGGCGA+TGG 0.495881 4_2:+69620305 None:intergenic
TTGTATCTAAAACAACCTCA+TGG 0.499097 4_2:+69622470 None:intergenic
CCCCAAACAATTGCAATCCC+AGG 0.504953 4_2:+69622174 None:intergenic
GAGTCGTTTATTTCAGGTTG+AGG 0.505598 4_2:-69622591 None:intergenic
TACTCATTGTGTACAAAAGC+TGG 0.505829 4_2:+69620369 None:intergenic
GTGCAGAAGACCTTGATTCA+AGG 0.506676 4_2:-69619849 Msa0618710:three_prime_UTR
CTTTGAGAAAGGATGCACTC+TGG 0.512629 4_2:+69620241 None:intergenic
ACAATGAGTACAGAGGAATA+TGG 0.514676 4_2:-69620356 Msa0618710:CDS
CTCAAAGAAGCTGGGCGTGG+TGG 0.514965 4_2:-69620225 Msa0618710:CDS
TCACAAACATGTGAAGGCTC+TGG 0.518719 4_2:-69622436 Msa0618710:CDS
ATGAACCCGGTTAGAACTCT+AGG 0.519479 4_2:-69620393 Msa0618710:CDS
AGTGTATATATGATTATCAA+GGG 0.526044 4_2:+69619771 None:intergenic
TTTCAGGAACACTTCCATCT+GGG 0.526344 4_2:-69620837 Msa0618710:CDS
TGTATCTAAAACAACCTCAT+GGG 0.536127 4_2:+69622471 None:intergenic
TCAAAGAAGCTGGGCGTGGT+GGG 0.536459 4_2:-69620224 Msa0618710:CDS
CTATATATGACCTTGAATCA+AGG 0.539301 4_2:+69619839 None:intergenic
AGCTGAGCTGATATATAAGC+TGG 0.541389 4_2:+69620922 None:intergenic
GCAAAAGCAATAGTAACAGC+AGG 0.549342 4_2:+69622093 None:intergenic
TTGGTATTGATTTACTCACT+TGG 0.550812 4_2:-69622141 Msa0618710:CDS
TTTCTCAAAGAAGCTGGGCG+TGG 0.552363 4_2:-69620228 Msa0618710:CDS
GTTTGTGTGTAGTAGTACTA+AGG 0.555212 4_2:-69620080 Msa0618710:three_prime_UTR
CATCCTTTCTCAAAGAAGCT+GGG 0.557103 4_2:-69620233 Msa0618710:CDS
TGAGTTTACCGCTCTAGTAT+CGG 0.558198 4_2:+69620741 None:intergenic
CTCTGGGTGATCTCACGCAA+TGG 0.559604 4_2:+69620258 None:intergenic
CTGGTCCTAGAGTTCTAACC+GGG 0.561868 4_2:+69620388 None:intergenic
GGTAAACATAAGGTAAAATG+TGG 0.569076 4_2:+69620780 None:intergenic
ATGGAAGTGTTCCTGAAAAG+TGG 0.569487 4_2:+69620842 None:intergenic
TGCTTAATGTGATGATTGCA+GGG 0.570893 4_2:-69620594 Msa0618710:intron
TCGTTTATTTCAGGTTGAGG+AGG 0.578693 4_2:-69622588 Msa0618710:five_prime_UTR
GCCATAAGTGATAGAGAACA+AGG 0.580173 4_2:+69622520 None:intergenic
ACAACGTGGTAACACTTCCT+GGG 0.584721 4_2:-69622191 Msa0618710:CDS
ACAGTTAATAGCAGAAATGG+TGG 0.598028 4_2:-69622271 Msa0618710:intron
AGTGTTGTAGACCCATGCTC+CGG 0.603956 4_2:+69620299 None:intergenic
GCTTGAAGGTTAGACCCAGA+TGG 0.605252 4_2:+69620823 None:intergenic
TTGGGAGCAACACTTGCTAG+TGG 0.608245 4_2:-69620898 Msa0618710:CDS
ACTCTTAAACTAATATTCAG+TGG 0.615140 4_2:-69620874 Msa0618710:CDS
GATTCCTCACAAACATGTGA+AGG 0.617028 4_2:-69622442 Msa0618710:CDS
GTAGTGTTGACCTGTAACAA+TGG 0.623661 4_2:+69622054 None:intergenic
TGCTTCAATAGTAGTGAACA+AGG 0.624250 4_2:-69622220 Msa0618710:CDS
AACAGGAGCATCAATGAACC+CGG 0.626218 4_2:-69620406 Msa0618710:CDS
AGAGCCTTCACATGTTTGTG+AGG 0.626868 4_2:+69622438 None:intergenic
CAGTTAATAGCAGAAATGGT+GGG 0.636357 4_2:-69622270 Msa0618710:CDS
GTTTACCGCTCTAGTATCGG+TGG 0.679010 4_2:+69620744 None:intergenic
AGCAAGAATTGAAACCCATG+AGG 0.683901 4_2:-69622485 Msa0618710:CDS
TTTGTACACAATGAGTACAG+AGG 0.696529 4_2:-69620363 Msa0618710:CDS
TAACAATGGAAACCTCCTTG+TGG 0.700560 4_2:+69622068 None:intergenic
AGTTGCCACCGATACTAGAG+CGG 0.735236 4_2:-69620749 Msa0618710:intron
GAACAAGGACAACGACAACG+TGG 0.744853 4_2:-69622205 Msa0618710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAATATATATAAAAT+TGG - chr4_2:69621096-69621115 Msa0618710:intron 0.0%
!! TATAGTATATTGAATTAATT+AGG - chr4_2:69620054-69620073 Msa0618710:three_prime_UTR 10.0%
!! ATTTACAAAAATAAACTTTA+TGG + chr4_2:69620355-69620374 None:intergenic 10.0%
!!! AACTATTTTTTTTTTAATCT+TGG - chr4_2:69620459-69620478 Msa0618710:intron 10.0%
!!! TTTTTTTTTTTGGTAAAAAA+AGG + chr4_2:69620647-69620666 None:intergenic 10.0%
!!! GTTGTTTTTTTTTTTTTTTT+TGG + chr4_2:69620657-69620676 None:intergenic 10.0%
!!! TTTTTTGACAAAAAAAATTA+TGG - chr4_2:69621155-69621174 Msa0618710:intron 10.0%
!! AGAGTATATACATTTAAATA+AGG + chr4_2:69620426-69620445 None:intergenic 15.0%
!!! TTTTTTATTAGCTACTTTTT+GGG - chr4_2:69620483-69620502 Msa0618710:intron 15.0%
!! TTTACAAATTGAGTTAAAAA+GGG + chr4_2:69620587-69620606 None:intergenic 15.0%
!!! TTTTACAAATTGAGTTAAAA+AGG + chr4_2:69620588-69620607 None:intergenic 15.0%
!!! GGATTATTATATTTTTTTAC+AGG + chr4_2:69620626-69620645 None:intergenic 15.0%
!! AAAAATACATGATAAATCAA+TGG - chr4_2:69620731-69620750 Msa0618710:intron 15.0%
!!! TTCTTATACTTTTAATGTTT+TGG + chr4_2:69620965-69620984 None:intergenic 15.0%
!!! TATTTAGAATTCACTATTAT+TGG + chr4_2:69621072-69621091 None:intergenic 15.0%
!!! ATATTTTATGTTTTGTAGAT+TGG - chr4_2:69621715-69621734 Msa0618710:intron 15.0%
!!! AGTTTTGCAATTTTTTTTTA+TGG + chr4_2:69621851-69621870 None:intergenic 15.0%
!!! TGATAATGAGAAAATAAAAA+TGG - chr4_2:69622265-69622284 Msa0618710:CDS 15.0%
!!! TTAATTAGGTTTTGTTTTGT+TGG - chr4_2:69620068-69620087 Msa0618710:three_prime_UTR 20.0%
!!! CTTTTTTATTAGCTACTTTT+TGG - chr4_2:69620482-69620501 Msa0618710:intron 20.0%
!! TTACAAATTGAGTTAAAAAG+GGG + chr4_2:69620586-69620605 None:intergenic 20.0%
!!! GTATTTTTACAATGATAAGT+TGG + chr4_2:69620719-69620738 None:intergenic 20.0%
!! ACAATATATAGAATAACAAG+TGG - chr4_2:69620848-69620867 Msa0618710:CDS 20.0%
!!! TGATATTTTCATGTAAAATC+AGG + chr4_2:69621021-69621040 None:intergenic 20.0%
!!! TTGGTTTAAAATTAATGTCA+AGG + chr4_2:69621053-69621072 None:intergenic 20.0%
!!! CCTAATAAATTGAAGAATTT+TGG + chr4_2:69621277-69621296 None:intergenic 20.0%
!! CCAAAATTCTTCAATTTATT+AGG - chr4_2:69621274-69621293 Msa0618710:intron 20.0%
!! AATTAAGTAACATGATTTCT+TGG + chr4_2:69621356-69621375 None:intergenic 20.0%
!! ATCCTTTGTATAATTCTTAT+AGG - chr4_2:69621420-69621439 Msa0618710:intron 20.0%
!! TACCTATAAGAATTATACAA+AGG + chr4_2:69621425-69621444 None:intergenic 20.0%
!!! TAGTAATTTTGCATTTAACT+AGG + chr4_2:69621821-69621840 None:intergenic 20.0%
!! AACCAAATAGTTTAAGAAAT+TGG - chr4_2:69622166-69622185 Msa0618710:CDS 20.0%
!! TTCCAATTTCTTAAACTATT+TGG + chr4_2:69622171-69622190 None:intergenic 20.0%
!!! TACACTCTCTATATTTATTT+TGG + chr4_2:69622475-69622494 None:intergenic 20.0%
! AATACAGTTAATAGCAGAAA+TGG - chr4_2:69620093-69620112 Msa0618710:three_prime_UTR 25.0%
! ACATATTTCTTGATATTTGC+AGG - chr4_2:69620121-69620140 Msa0618710:three_prime_UTR 25.0%
! AAAGAGTCTTAATTTCTTGT+GGG + chr4_2:69620525-69620544 None:intergenic 25.0%
! TAAAGAGTCTTAATTTCTTG+TGG + chr4_2:69620526-69620545 None:intergenic 25.0%
! AATTCAATTTCAAGAGAAGT+GGG + chr4_2:69620552-69620571 None:intergenic 25.0%
! AAATTCAATTTCAAGAGAAG+TGG + chr4_2:69620553-69620572 None:intergenic 25.0%
! ACTTCTCTTGAAATTGAATT+TGG - chr4_2:69620552-69620571 Msa0618710:intron 25.0%
! TACAAATTGAGTTAAAAAGG+GGG + chr4_2:69620585-69620604 None:intergenic 25.0%
! GAGAAAATATATTCAGACTT+AGG + chr4_2:69620681-69620700 None:intergenic 25.0%
!! TTAATCCAACCCAAATTTTT+AGG - chr4_2:69620935-69620954 Msa0618710:intron 25.0%
!! TAATCCAACCCAAATTTTTA+GGG - chr4_2:69620936-69620955 Msa0618710:intron 25.0%
!! TTTGGTAACCCTAAAAATTT+GGG + chr4_2:69620947-69620966 None:intergenic 25.0%
!!! TTTTGGTAACCCTAAAAATT+TGG + chr4_2:69620948-69620967 None:intergenic 25.0%
!! TCTCATTGGTAATAAAATGA+TGG - chr4_2:69621125-69621144 Msa0618710:intron 25.0%
!! TTTCTATTTGGGAGTTTTTA+CGG + chr4_2:69621302-69621321 None:intergenic 25.0%
!!! TTCAAGGAGTATTTCTATTT+GGG + chr4_2:69621313-69621332 None:intergenic 25.0%
!!! ATTCAAGGAGTATTTCTATT+TGG + chr4_2:69621314-69621333 None:intergenic 25.0%
!!! ACATTTTAGCTACTTATTCA+AGG + chr4_2:69621329-69621348 None:intergenic 25.0%
! TGATTCATAATTGCATAACA+TGG + chr4_2:69621401-69621420 None:intergenic 25.0%
! ACTCTTAAACTAATATTCAG+TGG - chr4_2:69621493-69621512 Msa0618710:intron 25.0%
!!! ATTTAACTAGGTTCTAGATA+GGG + chr4_2:69621809-69621828 None:intergenic 25.0%
!!! ATGTCACTTTTTTATTTGAC+AGG - chr4_2:69621933-69621952 Msa0618710:intron 25.0%
! ATCAATTTAGCACCTTAATT+AGG + chr4_2:69622370-69622389 None:intergenic 25.0%
!! TAATTAAGGTGCTAAATTGA+TGG - chr4_2:69622369-69622388 Msa0618710:intron 25.0%
!!! ATTTTGGATTTTTGTTTCTC+TGG + chr4_2:69622459-69622478 None:intergenic 25.0%
! TGTATCTAAAACAACCTCAT+GGG + chr4_2:69619899-69619918 None:intergenic 30.0%
! TTGTATCTAAAACAACCTCA+TGG + chr4_2:69619900-69619919 None:intergenic 30.0%
!!! GGTTGTTTTAGATACAAACA+AGG - chr4_2:69619903-69619922 Msa0618710:three_prime_UTR 30.0%
GAGTTTGTAAAGGAAAGATT+AGG + chr4_2:69619994-69620013 None:intergenic 30.0%
CTATGTGTATGAGTTTGTAA+AGG + chr4_2:69620004-69620023 None:intergenic 30.0%
!! TTGGTATTGATTTACTCACT+TGG - chr4_2:69620226-69620245 Msa0618710:CDS 30.0%
!! TTTTGGGTATAATTATCTGC+AGG - chr4_2:69620499-69620518 Msa0618710:intron 30.0%
GAAGTTTGATTCATTACTCT+CGG - chr4_2:69620874-69620893 Msa0618710:CDS 30.0%
AAAATTGGCATAACTCTCAT+TGG - chr4_2:69621111-69621130 Msa0618710:intron 30.0%
GTGATGTTTCTTAAAGCATA+TGG - chr4_2:69621191-69621210 Msa0618710:intron 30.0%
TTCATAATTGCATAACATGG+TGG + chr4_2:69621398-69621417 None:intergenic 30.0%
ATTCAAGAACAAAAGCTTGA+AGG + chr4_2:69621561-69621580 None:intergenic 30.0%
GGTAAACATAAGGTAAAATG+TGG + chr4_2:69621590-69621609 None:intergenic 30.0%
TACCTTATGTTTACCATTTC+TGG - chr4_2:69621595-69621614 Msa0618710:intron 30.0%
!! CATTTAACTAGGTTCTAGAT+AGG + chr4_2:69621810-69621829 None:intergenic 30.0%
!! GGTAGAAAGAAAACTAGTTT+TGG + chr4_2:69622438-69622457 None:intergenic 30.0%
CTGCACATTAAAAGAAACTT+AGG + chr4_2:69622504-69622523 None:intergenic 30.0%
CTATATATGACCTTGAATCA+AGG + chr4_2:69622531-69622550 None:intergenic 30.0%
TCCTTGTTCTCTATCACTTA+TGG - chr4_2:69619846-69619865 Msa0618710:three_prime_UTR 35.0%
ACAGTTAATAGCAGAAATGG+TGG - chr4_2:69620096-69620115 Msa0618710:three_prime_UTR 35.0%
CAGTTAATAGCAGAAATGGT+GGG - chr4_2:69620097-69620116 Msa0618710:three_prime_UTR 35.0%
TGCTTCAATAGTAGTGAACA+AGG - chr4_2:69620147-69620166 Msa0618710:three_prime_UTR 35.0%
! AATGATAAGTTGGCGTGAAA+AGG + chr4_2:69620709-69620728 None:intergenic 35.0%
GTAACCCTAAAAATTTGGGT+TGG + chr4_2:69620943-69620962 None:intergenic 35.0%
TTATATATCAGCTCAGCTCT+TGG - chr4_2:69621450-69621469 Msa0618710:intron 35.0%
TATATATCAGCTCAGCTCTT+GGG - chr4_2:69621451-69621470 Msa0618710:intron 35.0%
CTCCAGAAATGGTAAACATA+AGG + chr4_2:69621600-69621619 None:intergenic 35.0%
! TGTTTTGTAGATTGGTGAGT+TGG - chr4_2:69621723-69621742 Msa0618710:intron 35.0%
TTGCTTAATGTGATGATTGC+AGG - chr4_2:69621772-69621791 Msa0618710:intron 35.0%
TGCTTAATGTGATGATTGCA+GGG - chr4_2:69621773-69621792 Msa0618710:intron 35.0%
!!! CACTTTTTTATTTGACAGGC+CGG - chr4_2:69621937-69621956 Msa0618710:intron 35.0%
TACTCATTGTGTACAAAAGC+TGG + chr4_2:69622001-69622020 None:intergenic 35.0%
TTTGTACACAATGAGTACAG+AGG - chr4_2:69622004-69622023 Msa0618710:intron 35.0%
ACAATGAGTACAGAGGAATA+TGG - chr4_2:69622011-69622030 Msa0618710:intron 35.0%
!!! ATATTTGCTGTCACCGATTT+TGG - chr4_2:69622036-69622055 Msa0618710:intron 35.0%
!!! TATTTGCTGTCACCGATTTT+GGG - chr4_2:69622037-69622056 Msa0618710:intron 35.0%
TCTGCATACATGATACTTGA+TGG + chr4_2:69622202-69622221 None:intergenic 35.0%
GTTTGTGTGTAGTAGTACTA+AGG - chr4_2:69622287-69622306 Msa0618710:intron 35.0%
!!! TGATCCTGTGCAGTTTTAAA+AGG - chr4_2:69622322-69622341 Msa0618710:intron 35.0%
CTGCATACACTTCCTAATTA+AGG - chr4_2:69622355-69622374 Msa0618710:intron 35.0%
GCCATAAGTGATAGAGAACA+AGG + chr4_2:69619850-69619869 None:intergenic 40.0%
AGCAAGAATTGAAACCCATG+AGG - chr4_2:69619882-69619901 Msa0618710:three_prime_UTR 40.0%
GATTCCTCACAAACATGTGA+AGG - chr4_2:69619925-69619944 Msa0618710:three_prime_UTR 40.0%
CAAGAAAGGGACAGAGATAA+AGG + chr4_2:69619959-69619978 None:intergenic 40.0%
! GAGACACCTTTTGCAAGAAA+GGG + chr4_2:69619972-69619991 None:intergenic 40.0%
TTCCTGGGATTGCAATTGTT+TGG - chr4_2:69620191-69620210 Msa0618710:exon 40.0%
TCCTGGGATTGCAATTGTTT+GGG - chr4_2:69620192-69620211 Msa0618710:exon 40.0%
TGTTTGGGGACTAACTTTGT+TGG - chr4_2:69620207-69620226 Msa0618710:exon 40.0%
TACTCACTTGGTCATATCTC+TGG - chr4_2:69620238-69620257 Msa0618710:CDS 40.0%
GCAAAAGCAATAGTAACAGC+AGG + chr4_2:69620277-69620296 None:intergenic 40.0%
!! CTATTGCTTTTGCTACCACA+AGG - chr4_2:69620284-69620303 Msa0618710:CDS 40.0%
TAACAATGGAAACCTCCTTG+TGG + chr4_2:69620302-69620321 None:intergenic 40.0%
AAGGAGGTTTCCATTGTTAC+AGG - chr4_2:69620303-69620322 Msa0618710:CDS 40.0%
!! GTAGTGTTGACCTGTAACAA+TGG + chr4_2:69620316-69620335 None:intergenic 40.0%
AGCTGAGCTGATATATAAGC+TGG + chr4_2:69621448-69621467 None:intergenic 40.0%
!! ATGGAAGTGTTCCTGAAAAG+TGG + chr4_2:69621528-69621547 None:intergenic 40.0%
!! TTTTCAGGAACACTTCCATC+TGG - chr4_2:69621529-69621548 Msa0618710:intron 40.0%
TTTCAGGAACACTTCCATCT+GGG - chr4_2:69621530-69621549 Msa0618710:intron 40.0%
TGAGTTTACCGCTCTAGTAT+CGG + chr4_2:69621629-69621648 None:intergenic 40.0%
AGTTGGCTGGAATTGCAATT+GGG - chr4_2:69621740-69621759 Msa0618710:intron 40.0%
TTCATTGATGCTCCTGTTAC+CGG + chr4_2:69621959-69621978 None:intergenic 40.0%
!!! ATTTGCTGTCACCGATTTTG+GGG - chr4_2:69622038-69622057 Msa0618710:intron 40.0%
TTTGAGAAAGGATGCACTCT+GGG + chr4_2:69622128-69622147 None:intergenic 40.0%
CATCCTTTCTCAAAGAAGCT+GGG - chr4_2:69622134-69622153 Msa0618710:CDS 40.0%
!!! AGCTCCTTTTAAAACTGCAC+AGG + chr4_2:69622329-69622348 None:intergenic 40.0%
AGAGCCTTCACATGTTTGTG+AGG + chr4_2:69619932-69619951 None:intergenic 45.0%
TCACAAACATGTGAAGGCTC+TGG - chr4_2:69619931-69619950 Msa0618710:three_prime_UTR 45.0%
CTCTGTCCCTTTCTTGCAAA+AGG - chr4_2:69619963-69619982 Msa0618710:three_prime_UTR 45.0%
! GGAGACACCTTTTGCAAGAA+AGG + chr4_2:69619973-69619992 None:intergenic 45.0%
ACAACGTGGTAACACTTCCT+GGG - chr4_2:69620176-69620195 Msa0618710:three_prime_UTR 45.0%
CCTGGGATTGCAATTGTTTG+GGG - chr4_2:69620193-69620212 Msa0618710:exon 45.0%
!! TTGCTTTTGCTACCACAAGG+AGG - chr4_2:69620287-69620306 Msa0618710:CDS 45.0%
! TTGTAGATTGGTGAGTTGGC+TGG - chr4_2:69621727-69621746 Msa0618710:intron 45.0%
GAGTTGGCTGGAATTGCAAT+TGG - chr4_2:69621739-69621758 Msa0618710:intron 45.0%
TTATTTGACAGGCCGGTAAC+AGG - chr4_2:69621944-69621963 Msa0618710:intron 45.0%
!! AACAGGAGCATCAATGAACC+CGG - chr4_2:69621961-69621980 Msa0618710:intron 45.0%
ATGAACCCGGTTAGAACTCT+AGG - chr4_2:69621974-69621993 Msa0618710:intron 45.0%
!!! TTTGCTGTCACCGATTTTGG+GGG - chr4_2:69622039-69622058 Msa0618710:intron 45.0%
CATGGGTCTACAACACTGTT+AGG - chr4_2:69622074-69622093 Msa0618710:CDS 45.0%
! CTTTGAGAAAGGATGCACTC+TGG + chr4_2:69622129-69622148 None:intergenic 45.0%
GCATCCTTTCTCAAAGAAGC+TGG - chr4_2:69622133-69622152 Msa0618710:CDS 45.0%
GTGCAGAAGACCTTGATTCA+AGG - chr4_2:69622518-69622537 Msa0618710:exon 45.0%
GAACAAGGACAACGACAACG+TGG - chr4_2:69620162-69620181 Msa0618710:three_prime_UTR 50.0%
GACAACGTGGTAACACTTCC+TGG - chr4_2:69620175-69620194 Msa0618710:three_prime_UTR 50.0%
CCCCAAACAATTGCAATCCC+AGG + chr4_2:69620196-69620215 None:intergenic 50.0%
! TTGGGAGCAACACTTGCTAG+TGG - chr4_2:69621469-69621488 Msa0618710:intron 50.0%
!! GGCACACATGACCACTTTTC+AGG - chr4_2:69621514-69621533 Msa0618710:intron 50.0%
GCTTGAAGGTTAGACCCAGA+TGG + chr4_2:69621547-69621566 None:intergenic 50.0%
ATCGGTGGCAACTCCAGAAA+TGG + chr4_2:69621611-69621630 None:intergenic 50.0%
AGTTGCCACCGATACTAGAG+CGG - chr4_2:69621618-69621637 Msa0618710:intron 50.0%
! GTTTACCGCTCTAGTATCGG+TGG + chr4_2:69621626-69621645 None:intergenic 50.0%
! CTGGTCCTAGAGTTCTAACC+GGG + chr4_2:69621982-69622001 None:intergenic 50.0%
! GCTGGTCCTAGAGTTCTAAC+CGG + chr4_2:69621983-69622002 None:intergenic 50.0%
AGTGTTGTAGACCCATGCTC+CGG + chr4_2:69622071-69622090 None:intergenic 50.0%
! ACGCCCAGCTTCTTTGAGAA+AGG + chr4_2:69622140-69622159 None:intergenic 50.0%
TTTCTCAAAGAAGCTGGGCG+TGG - chr4_2:69622139-69622158 Msa0618710:CDS 50.0%
CTCTGGGTGATCTCACGCAA+TGG + chr4_2:69622112-69622131 None:intergenic 55.0%
TCAAAGAAGCTGGGCGTGGT+GGG - chr4_2:69622143-69622162 Msa0618710:CDS 55.0%
CTCAAAGAAGCTGGGCGTGG+TGG - chr4_2:69622142-69622161 Msa0618710:CDS 60.0%
CCGGCGATGGCCCCCAAAAT+CGG + chr4_2:69622052-69622071 None:intergenic 65.0%
!! CCGATTTTGGGGGCCATCGC+CGG - chr4_2:69622049-69622068 Msa0618710:intron 65.0%
GTAGACCCATGCTCCGGCGA+TGG + chr4_2:69622065-69622084 None:intergenic 65.0%
TGGGGGCCATCGCCGGAGCA+TGG - chr4_2:69622056-69622075 Msa0618710:intron 75.0%
GGGGGCCATCGCCGGAGCAT+GGG - chr4_2:69622057-69622076 Msa0618710:intron 75.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 69619781 69622608 69619781 ID=Msa0618710;Name=Msa0618710
chr4_2 mRNA 69619781 69622608 69619781 ID=Msa0618710-mRNA-1;Parent=Msa0618710;Name=Msa0618710-mRNA-1;_AED=0.26;_eAED=0.26;_QI=84|1|1|1|1|1|5|428|276
chr4_2 exon 69619781 69620434 69619781 ID=Msa0618710-mRNA-1:exon:20180;Parent=Msa0618710-mRNA-1
chr4_2 exon 69620595 69620656 69620595 ID=Msa0618710-mRNA-1:exon:20179;Parent=Msa0618710-mRNA-1
chr4_2 exon 69620750 69620947 69620750 ID=Msa0618710-mRNA-1:exon:20178;Parent=Msa0618710-mRNA-1
chr4_2 exon 69622065 69622289 69622065 ID=Msa0618710-mRNA-1:exon:20177;Parent=Msa0618710-mRNA-1
chr4_2 exon 69622405 69622608 69622405 ID=Msa0618710-mRNA-1:exon:20176;Parent=Msa0618710-mRNA-1
chr4_2 five_prime_UTR 69622525 69622608 69622525 ID=Msa0618710-mRNA-1:five_prime_utr;Parent=Msa0618710-mRNA-1
chr4_2 CDS 69622405 69622524 69622405 ID=Msa0618710-mRNA-1:cds;Parent=Msa0618710-mRNA-1
chr4_2 CDS 69622065 69622289 69622065 ID=Msa0618710-mRNA-1:cds;Parent=Msa0618710-mRNA-1
chr4_2 CDS 69620750 69620947 69620750 ID=Msa0618710-mRNA-1:cds;Parent=Msa0618710-mRNA-1
chr4_2 CDS 69620595 69620656 69620595 ID=Msa0618710-mRNA-1:cds;Parent=Msa0618710-mRNA-1
chr4_2 CDS 69620209 69620434 69620209 ID=Msa0618710-mRNA-1:cds;Parent=Msa0618710-mRNA-1
chr4_2 three_prime_UTR 69619781 69620208 69619781 ID=Msa0618710-mRNA-1:three_prime_utr;Parent=Msa0618710-mRNA-1
Gene Sequence

>Msa0618710

ATGGCAAATGATAATTCAGCAAGAATTGAAACCCATGAGGTTGTTTTAGATACAAACAAGGATTCCTCACAAACATGTGAAGGCTCTGGCTCCTTTATCTCTGTCCCTTTCTTGCAAAAGTTAATAGCAGAAATGGTGGGCACATATTTCTTGATATTTGCAGGATGTGCTTCAATAGTAGTGAACAAGGACAACGACAACGTGGTAACACTTCCTGGGATTGCAATTGTTTGGGGACTAACTTTGTTGGTATTGATTTACTCACTTGGTCATATCTCTGGTGCTCATTTCAATCCTGCTGTTACTATTGCTTTTGCTACCACAAGGAGGTTTCCATTGTTACAGGTACCAGCTTATATATCAGCTCAGCTCTTGGGAGCAACACTTGCTAGTGGAACTCTTAAACTAATATTCAGTGGCACACATGACCACTTTTCAGGAACACTTCCATCTGGGTCTAACCTTCAAGCTTTTGTTCTTGAATTCATAACCACATTTTACCTTATGTTTACCATTTCTGGAGTTGCCACCGATACTAGAGCGATTGGTGAGTTGGCTGGAATTGCAATTGGGTCTACATTATTGCTTAATGTGATGATTGCAGGGCCGGTAACAGGAGCATCAATGAACCCGGTTAGAACTCTAGGACCAGCTTTTGTACACAATGAGTACAGAGGAATATGGATATATTTGCTGTCACCGATTTTGGGGGCCATCGCCGGAGCATGGGTCTACAACACTGTTAGGTACACTAACAAGCCATTGCGTGAGATCACCCAGAGTGCATCCTTTCTCAAAGAAGCTGGGCGTGGTGGGAACCAAATAGTTTAA
Protein sequence

>Msa0618710

MANDNSARIETHEVVLDTNKDSSQTCEGSGSFISVPFLQKLIAEMVGTYFLIFAGCASIVVNKDNDNVVTLPGIAIVWGLTLLVLIYSLGHISGAHFNPAVTIAFATTRRFPLLQVPAYISAQLLGATLASGTLKLIFSGTHDHFSGTLPSGSNLQAFVLEFITTFYLMFTISGVATDTRAIGELAGIAIGSTLLLNVMIAGPVTGASMNPVRTLGPAFVHNEYRGIWIYLLSPILGAIAGAWVYNTVRYTNKPLREITQSASFLKEAGRGGNQIV*