AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0621970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0621970 MtrunA17_Chr7g0272351 95.652 92 4 0 1 92 1 92 1.60e-59 179
Msa0621970 MtrunA17_Chr1g0192251 66.990 103 30 3 1 101 1 101 5.52e-37 122
Msa0621970 MtrunA17_Chr8g0344711 39.316 117 53 5 1 117 1 99 6.37e-13 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0621970 AT1G56220.2 72.093 86 22 2 1 84 1 86 1.70e-36 121
Msa0621970 AT1G56220.4 72.093 86 22 2 1 84 26 111 2.05e-36 121
Msa0621970 AT1G56220.1 72.093 86 22 2 1 84 1 86 4.15e-36 120
Msa0621970 AT1G56220.3 72.093 86 22 2 1 84 26 111 4.48e-36 121
Msa0621970 AT1G56220.5 72.093 86 22 2 1 84 26 111 4.79e-36 121
Msa0621970 AT1G54070.1 47.761 67 21 2 1 67 1 53 1.90e-11 57.4

Find 96 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGCATATTGAATAATTT+TGG 0.110350 4_2:+74300782 None:intergenic
TCTATATTCTCGATCCATTT+TGG 0.241117 4_2:-74300909 Msa0621970:three_prime_UTR
ACTGAAGATGTTACCCCTTT+TGG 0.271295 4_2:-74301291 Msa0621970:three_prime_UTR
GGGGTAACATCTTCAGTTTC+CGG 0.301859 4_2:+74301297 None:intergenic
ACGAGAGATTGTCAAGAAAA+AGG 0.303375 4_2:+74300951 None:intergenic
AAGAAGGGCCTGATCTGTTT+TGG 0.319411 4_2:+74301364 None:intergenic
AATTCATCCTCATACCAAAA+TGG 0.338716 4_2:+74300895 None:intergenic
AGTGAACAATGGAACAATAA+TGG 0.359458 4_2:-74300727 Msa0621970:three_prime_UTR
GTTAGGCATGGCGCTCTGTT+TGG 0.361146 4_2:-74301178 Msa0621970:three_prime_UTR
TGTGTATATAGATCCTTTAC+TGG 0.368772 4_2:-74301020 Msa0621970:three_prime_UTR
AGTGTAATAAGTTGTAAGTA+AGG 0.373833 4_2:-74301087 Msa0621970:three_prime_UTR
GTGAATAATAACGACCTTAC+CGG 0.378031 4_2:+74302413 None:intergenic
TACACCGCTATTCTACCCTT+TGG 0.402296 4_2:+74301060 None:intergenic
CGATCCCAATGACCAGATTC+CGG 0.418252 4_2:+74303205 None:intergenic
TACCCTTGCCAGAGCTAGAT+CGG 0.420036 4_2:+74303048 None:intergenic
CAGGAACTAGAGATTCCTTT+CGG 0.426315 4_2:-74301428 Msa0621970:CDS
CCGAGACCGTTCTCCGGCCG+AGG 0.426718 4_2:+74303101 None:intergenic
CGACGGTGTCGTCCCACAAC+TGG 0.429141 4_2:+74303129 None:intergenic
ACCGTTCTCCGGCCGAGGGC+CGG 0.433178 4_2:+74303106 None:intergenic
CTCCGGCCGAGGGCCGGCGA+CGG 0.445786 4_2:+74303112 None:intergenic
CACCGTCGCCGGCCCTCGGC+CGG 0.447404 4_2:-74303114 Msa0621970:CDS
GTATCTCTTACACCGGAATC+TGG 0.448206 4_2:-74303217 Msa0621970:five_prime_UTR
AGAACGGTCTCGGCAAGCTT+CGG 0.450915 4_2:-74303091 Msa0621970:CDS
TGGGCCTTCTCGACCAGTTG+TGG 0.456063 4_2:-74303142 Msa0621970:CDS
TTTGTGCTGCGGTTCACGTT+AGG 0.456617 4_2:-74301195 Msa0621970:three_prime_UTR
TACACCGGAATCTGGTCATT+GGG 0.458037 4_2:-74303209 Msa0621970:five_prime_UTR
TCGCCTCTGGCTTCTCCCGC+CGG 0.458567 4_2:-74302462 Msa0621970:CDS
AAATACGCCTGATCCATTCA+TGG 0.461198 4_2:-74303162 Msa0621970:exon
TATTACAGCAAAGGCCAAAA+GGG 0.461978 4_2:+74301277 None:intergenic
TTACACCGGAATCTGGTCAT+TGG 0.463228 4_2:-74303210 Msa0621970:five_prime_UTR
ACGACACCGTCGCCGGCCCT+CGG 0.467035 4_2:-74303118 Msa0621970:CDS
CCTCGGCCGGAGAACGGTCT+CGG 0.467575 4_2:-74303101 Msa0621970:CDS
TCAATATGCATGTCGGAAGA+TGG 0.467631 4_2:-74300772 Msa0621970:three_prime_UTR
TTATTACAGCAAAGGCCAAA+AGG 0.472228 4_2:+74301276 None:intergenic
CGCATTCAATCAGTGAACAA+TGG 0.473374 4_2:-74300738 Msa0621970:three_prime_UTR
CTTTCGGTTTCGACGAAGGT+CGG 0.476530 4_2:-74301412 Msa0621970:CDS
GCCGGCCCTCGGCCGGAGAA+CGG 0.477433 4_2:-74303107 Msa0621970:CDS
AGAAGGCCCATGAATGGATC+AGG 0.480654 4_2:+74303155 None:intergenic
ATAACGACCTTACCGGAAAA+CGG 0.482174 4_2:+74302420 None:intergenic
GTACGAAAGTGGATCGCCTC+TGG 0.484048 4_2:-74302475 Msa0621970:CDS
CGCCCGATCTAGCTCTGGCA+AGG 0.484849 4_2:-74303051 Msa0621970:CDS
AAGAAAGGCCAAAACAGATC+AGG 0.489997 4_2:-74301372 Msa0621970:CDS
ATCATGATCGTGAAACCGCC+AGG 0.492141 4_2:-74302498 Msa0621970:CDS
TTCGCCGCCCGATCTAGCTC+TGG 0.500632 4_2:-74303056 Msa0621970:CDS
AACAATAATGGACATGAAGA+AGG 0.503571 4_2:-74300715 Msa0621970:three_prime_UTR
GGGCCTTCTCGACCAGTTGT+GGG 0.507384 4_2:-74303141 Msa0621970:CDS
AGAGCTAGATCGGGCGGCGA+AGG 0.508256 4_2:+74303058 None:intergenic
TGTAAGTAAGGAAGTCCAAA+GGG 0.513200 4_2:-74301075 Msa0621970:three_prime_UTR
CGCAGTAGTATCTCTTACAC+CGG 0.520795 4_2:-74303224 Msa0621970:five_prime_UTR
TGGTCGAGAAGGCCCATGAA+TGG 0.522793 4_2:+74303149 None:intergenic
GTTTCGACGAAGGTCGGCAT+CGG 0.527766 4_2:-74301406 Msa0621970:CDS
CACACATCGATAGGAGAAGA+AGG 0.528061 4_2:+74301348 None:intergenic
ATAATGGACATGAAGAAGGT+TGG 0.529729 4_2:-74300711 Msa0621970:three_prime_UTR
ATTCCTTTCGGTTTCGACGA+AGG 0.530725 4_2:-74301416 Msa0621970:CDS
TCCCGCCGGTTCTACTACTC+CGG 0.530743 4_2:-74302448 Msa0621970:CDS
GTTTATTTGTGTGCATGTAT+CGG 0.533623 4_2:-74301243 Msa0621970:three_prime_UTR
CAATATGCATGTCGGAAGAT+GGG 0.533830 4_2:-74300771 Msa0621970:three_prime_UTR
ACCCTTGCCAGAGCTAGATC+GGG 0.537273 4_2:+74303049 None:intergenic
AGCTTGCCGAGACCGTTCTC+CGG 0.541689 4_2:+74303095 None:intergenic
GCTGCGGTTCACGTTAGGCA+TGG 0.543848 4_2:-74301190 Msa0621970:three_prime_UTR
GCGGGAAGCGTGAGATCGTA+CGG 0.546978 4_2:-74302561 Msa0621970:CDS
ATCGTACGGTGAGGAATCGT+CGG 0.548167 4_2:-74302547 Msa0621970:CDS
AGACACCGGAGTAGTAGAAC+CGG 0.549756 4_2:+74302443 None:intergenic
AAACCGCCAGGGTACGAAAG+TGG 0.556609 4_2:-74302486 Msa0621970:CDS
GAACCGGCGGGAGAAGCCAG+AGG 0.561661 4_2:+74302459 None:intergenic
CGAGACCGTTCTCCGGCCGA+GGG 0.565198 4_2:+74303102 None:intergenic
AATACGCCTGATCCATTCAT+GGG 0.567709 4_2:-74303161 Msa0621970:exon
GGCGATCCACTTTCGTACCC+TGG 0.568040 4_2:+74302480 None:intergenic
CGACCTTCGTCGAAACCGAA+AGG 0.571902 4_2:+74301413 None:intergenic
ATGGATCGAGAATATAGAGC+AGG 0.575307 4_2:+74300914 None:intergenic
TCGGATGCATACGAGAAGAA+AGG 0.583908 4_2:-74301387 Msa0621970:CDS
AAATTATTCAATATGCATGT+CGG 0.584174 4_2:-74300779 Msa0621970:three_prime_UTR
GCCCGATCTAGCTCTGGCAA+GGG 0.585634 4_2:-74303050 Msa0621970:intron
GATTGATCAGAAATGGAGGC+GGG 0.586985 4_2:-74302579 Msa0621970:intron
CGTCCCACAACTGGTCGAGA+AGG 0.590163 4_2:+74303138 None:intergenic
CATGTATCGGTGTAATGCAT+TGG 0.591207 4_2:-74301230 Msa0621970:three_prime_UTR
TTGTGGGACGACACCGTCGC+CGG 0.591995 4_2:-74303125 Msa0621970:CDS
ACACTTGATAATGAGACAGC+CGG 0.594836 4_2:-74300810 Msa0621970:three_prime_UTR
TTGTAAGTAAGGAAGTCCAA+AGG 0.595220 4_2:-74301076 Msa0621970:three_prime_UTR
CTCATTTCTCACACATCGAT+AGG 0.601241 4_2:+74301339 None:intergenic
TGATTGATCAGAAATGGAGG+CGG 0.603270 4_2:-74302580 Msa0621970:intron
GTGTATATAGATCCTTTACT+GGG 0.611023 4_2:-74301019 Msa0621970:three_prime_UTR
TCTTCATGGGATGGCTACAG+AGG 0.611027 4_2:-74300838 Msa0621970:three_prime_UTR
AATTGATTGATCAGAAATGG+AGG 0.625094 4_2:-74302583 Msa0621970:intron
TTACCGGAAAACGGAGACAC+CGG 0.636278 4_2:+74302429 None:intergenic
ACACATCGATAGGAGAAGAA+GGG 0.647952 4_2:+74301349 None:intergenic
CACCGGAGTAGTAGAACCGG+CGG 0.650193 4_2:+74302446 None:intergenic
ATTACAGCAAAGGCCAAAAG+GGG 0.651252 4_2:+74301278 None:intergenic
TCATGATCGTGAAACCGCCA+GGG 0.653940 4_2:-74302497 Msa0621970:CDS
CTTGCCAGAGCTAGATCGGG+CGG 0.658767 4_2:+74303052 None:intergenic
ACCGGAGTAGTAGAACCGGC+GGG 0.676139 4_2:+74302447 None:intergenic
CCGGAGAAGATAAAATGCGG+TGG 0.684216 4_2:+74301316 None:intergenic
AAGTCCAAAGGGTAGAATAG+CGG 0.707284 4_2:-74301064 Msa0621970:three_prime_UTR
GATCCACTTTCGTACCCTGG+CGG 0.709901 4_2:+74302483 None:intergenic
AAGCGTGAGATCGTACGGTG+AGG 0.726983 4_2:-74302556 Msa0621970:CDS
TTTCCGGAGAAGATAAAATG+CGG 0.732385 4_2:+74301313 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTAATAAGTTTTTTTTGA+AGG - chr4_2:74301674-74301693 Msa0621970:intron 10.0%
!! ATTATATTGTCATATATTTA+AGG - chr4_2:74302227-74302246 Msa0621970:intron 10.0%
!! ATCATTTATAATAACAATAA+TGG - chr4_2:74302307-74302326 Msa0621970:intron 10.0%
!! AAAAAAAAAAACTTATTACA+CGG + chr4_2:74302783-74302802 None:intergenic 10.0%
!!! GTTTTTTTTTTTTTTTTTGA+AGG - chr4_2:74302791-74302810 Msa0621970:intron 10.0%
!! AAATTATATACGAGAAATTA+AGG + chr4_2:74300897-74300916 None:intergenic 15.0%
!! AAAATTTAAAAGCAAAAGTT+AGG + chr4_2:74301646-74301665 None:intergenic 15.0%
!! TTGAAGGAATAATTAATATT+AGG - chr4_2:74301690-74301709 Msa0621970:intron 15.0%
!! AACCAACTTATTAATATTAA+GGG + chr4_2:74301995-74302014 None:intergenic 15.0%
!! TAACCAACTTATTAATATTA+AGG + chr4_2:74301996-74302015 None:intergenic 15.0%
!!! TTTATAATAACAATAATGGA+AGG - chr4_2:74302311-74302330 Msa0621970:intron 15.0%
!! TGAAGGTTAATCAATATTAA+CGG + chr4_2:74301018-74301037 None:intergenic 20.0%
!!! AAGCTTAAAGTTTGAAATTA+TGG - chr4_2:74301093-74301112 Msa0621970:three_prime_UTR 20.0%
!! ATTAGGCTTAAATATGTTAT+TGG - chr4_2:74301707-74301726 Msa0621970:intron 20.0%
!! GAAACATTTATTGAAATGTA+AGG - chr4_2:74301921-74301940 Msa0621970:intron 20.0%
!!! TTAAGGGTTAAATATGTTTT+TGG + chr4_2:74301979-74301998 None:intergenic 20.0%
!! AACCCTTAATATTAATAAGT+TGG - chr4_2:74301990-74302009 Msa0621970:intron 20.0%
!! GAAAAAAATTATTACAGCAA+AGG + chr4_2:74302636-74302655 None:intergenic 20.0%
!!! AGATTCTTTTAAGTCTTATT+AGG - chr4_2:74302753-74302772 Msa0621970:intron 20.0%
!!! CATGCATATTGAATAATTTT+GGG + chr4_2:74303121-74303140 None:intergenic 20.0%
!!! ACATGCATATTGAATAATTT+TGG + chr4_2:74303122-74303141 None:intergenic 20.0%
!! AAATTATTCAATATGCATGT+CGG - chr4_2:74303122-74303141 Msa0621970:CDS 20.0%
! TTATATACGAGAAATTAAGG+TGG + chr4_2:74300894-74300913 None:intergenic 25.0%
! CAATTCGATATTCAATTTGA+AGG + chr4_2:74301035-74301054 None:intergenic 25.0%
! AAATCTATCTTGATTCTTGA+AGG - chr4_2:74301142-74301161 Msa0621970:three_prime_UTR 25.0%
! GAAAATTGATTGATCAGAAA+TGG - chr4_2:74301315-74301334 Msa0621970:three_prime_UTR 25.0%
! TTTACTTCACTTTACTTTAC+TGG + chr4_2:74301574-74301593 None:intergenic 25.0%
! GTAAATGATCTCCTAAATTT+CGG - chr4_2:74301589-74301608 Msa0621970:intron 25.0%
! TAAATGATCTCCTAAATTTC+GGG - chr4_2:74301590-74301609 Msa0621970:intron 25.0%
!! TGCTATATTTTGTCTTTTTG+TGG - chr4_2:74301615-74301634 Msa0621970:intron 25.0%
!! TATTTTGTCTTTTTGTGGAA+TGG - chr4_2:74301620-74301639 Msa0621970:intron 25.0%
!! ATACTACGGTTTCTAAATTT+TGG + chr4_2:74301852-74301871 None:intergenic 25.0%
! CAAAACTTCAACAAATACTA+CGG + chr4_2:74301866-74301885 None:intergenic 25.0%
! TCAAAGTCGTGTAAAATATA+TGG - chr4_2:74301952-74301971 Msa0621970:intron 25.0%
!!! GTGCTATTTTATTAATTGTG+TGG - chr4_2:74302167-74302186 Msa0621970:intron 25.0%
!!! CTAGTTGTCTATTTTAACTT+GGG + chr4_2:74302263-74302282 None:intergenic 25.0%
! AGTGTAATAAGTTGTAAGTA+AGG - chr4_2:74302814-74302833 Msa0621970:intron 25.0%
!!! CATATTGAATAATTTTGGGT+GGG + chr4_2:74303117-74303136 None:intergenic 25.0%
!! AATTTTAGCGATAACCGTTT+TGG + chr4_2:74300949-74300968 None:intergenic 30.0%
TGAATATCGAATTGTGTTCT+AGG - chr4_2:74301041-74301060 Msa0621970:three_prime_UTR 30.0%
!!! AGGTCTTGCATGTTTTATTT+TGG - chr4_2:74301162-74301181 Msa0621970:three_prime_UTR 30.0%
AATTGATTGATCAGAAATGG+AGG - chr4_2:74301318-74301337 Msa0621970:three_prime_UTR 30.0%
!!! CACTTTACTTTACTGGTTTT+TGG + chr4_2:74301567-74301586 None:intergenic 30.0%
!! GTTTCTAAATTTTGGTGACT+AGG + chr4_2:74301844-74301863 None:intergenic 30.0%
TTGTTCAACTTGAACAAAAC+CGG - chr4_2:74301883-74301902 Msa0621970:intron 30.0%
!! TTTCTAAAATCCCTACATTC+CGG + chr4_2:74301905-74301924 None:intergenic 30.0%
!!! CCTAGTTGTCTATTTTAACT+TGG + chr4_2:74302264-74302283 None:intergenic 30.0%
CCAAGTTAAAATAGACAACT+AGG - chr4_2:74302261-74302280 Msa0621970:intron 30.0%
GTTTATTTGTGTGCATGTAT+CGG - chr4_2:74302658-74302677 Msa0621970:intron 30.0%
TGTGTATATAGATCCTTTAC+TGG - chr4_2:74302881-74302900 Msa0621970:intron 30.0%
GTGTATATAGATCCTTTACT+GGG - chr4_2:74302882-74302901 Msa0621970:intron 30.0%
! TCTATATTCTCGATCCATTT+TGG - chr4_2:74302992-74303011 Msa0621970:intron 30.0%
AATTCATCCTCATACCAAAA+TGG + chr4_2:74303009-74303028 None:intergenic 30.0%
! TGGAACATTTTTGTCTTCAT+GGG - chr4_2:74303050-74303069 Msa0621970:intron 30.0%
! GCATATTGAATAATTTTGGG+TGG + chr4_2:74303118-74303137 None:intergenic 30.0%
AGTGAACAATGGAACAATAA+TGG - chr4_2:74303174-74303193 Msa0621970:five_prime_UTR 30.0%
!! AACAATAATGGACATGAAGA+AGG - chr4_2:74303186-74303205 Msa0621970:five_prime_UTR 30.0%
GTGATTCGTTTAACGAACAT+CGG + chr4_2:74300921-74300940 None:intergenic 35.0%
GTGAATAATAACGACCTTAC+CGG + chr4_2:74301491-74301510 None:intergenic 35.0%
! AGTTTTACCGTCAAGAAACA+CGG + chr4_2:74301529-74301548 None:intergenic 35.0%
!!! ACTTTACTGGTTTTTGGAAC+AGG + chr4_2:74301561-74301580 None:intergenic 35.0%
ATATAGCATGCCCGAAATTT+AGG + chr4_2:74301603-74301622 None:intergenic 35.0%
TCAAAGTTGCGCAAAATGTA+AGG + chr4_2:74301734-74301753 None:intergenic 35.0%
TTGAACAAAACCGGAATGTA+GGG - chr4_2:74301892-74301911 Msa0621970:intron 35.0%
GACAACTAGGTTAAGTTACT+TGG - chr4_2:74302274-74302293 Msa0621970:intron 35.0%
TTTCCGGAGAAGATAAAATG+CGG + chr4_2:74302591-74302610 None:intergenic 35.0%
TATTACAGCAAAGGCCAAAA+GGG + chr4_2:74302627-74302646 None:intergenic 35.0%
TTATTACAGCAAAGGCCAAA+AGG + chr4_2:74302628-74302647 None:intergenic 35.0%
!! TGAATTGAAGTTTTGTGCTG+CGG - chr4_2:74302695-74302714 Msa0621970:intron 35.0%
TTGTAAGTAAGGAAGTCCAA+AGG - chr4_2:74302825-74302844 Msa0621970:intron 35.0%
TGTAAGTAAGGAAGTCCAAA+GGG - chr4_2:74302826-74302845 Msa0621970:intron 35.0%
ACGAGAGATTGTCAAGAAAA+AGG + chr4_2:74302953-74302972 None:intergenic 35.0%
!!! AAGTCCAAGAAGTGTTTTTG+TGG - chr4_2:74303030-74303049 Msa0621970:intron 35.0%
TGTTCCACAAAAACACTTCT+TGG + chr4_2:74303037-74303056 None:intergenic 35.0%
! GTGGAACATTTTTGTCTTCA+TGG - chr4_2:74303049-74303068 Msa0621970:intron 35.0%
! ACATTTTTGTCTTCATGGGA+TGG - chr4_2:74303054-74303073 Msa0621970:CDS 35.0%
!! ATAATGGACATGAAGAAGGT+TGG - chr4_2:74303190-74303209 Msa0621970:five_prime_UTR 35.0%
AAATACGCCTGATCCATTCA+TGG - chr4_2:74300739-74300758 Msa0621970:three_prime_UTR 40.0%
AATACGCCTGATCCATTCAT+GGG - chr4_2:74300740-74300759 Msa0621970:three_prime_UTR 40.0%
ACGAATCACCAGAACCAAAA+CGG - chr4_2:74300932-74300951 Msa0621970:three_prime_UTR 40.0%
! TCTTTGAGCTTTTCACATGC+AGG + chr4_2:74301199-74301218 None:intergenic 40.0%
TGATTGATCAGAAATGGAGG+CGG - chr4_2:74301321-74301340 Msa0621970:exon 40.0%
ATAACGACCTTACCGGAAAA+CGG + chr4_2:74301484-74301503 None:intergenic 40.0%
CTTATACCCGTGTTTCTTGA+CGG - chr4_2:74301519-74301538 Msa0621970:intron 40.0%
! GTTTTACCGTCAAGAAACAC+GGG + chr4_2:74301528-74301547 None:intergenic 40.0%
GGTGACTAGGAAGAAATTGA+CGG + chr4_2:74301831-74301850 None:intergenic 40.0%
CTTGAACAAAACCGGAATGT+AGG - chr4_2:74301891-74301910 Msa0621970:intron 40.0%
!! GTAAGTTGATACGAGACTTG+TGG + chr4_2:74302023-74302042 None:intergenic 40.0%
GTCTCGTATCAACTTACAAG+CGG - chr4_2:74302026-74302045 Msa0621970:intron 40.0%
TTAAAACCCACACTCTTCCA+AGG + chr4_2:74302129-74302148 None:intergenic 40.0%
!! GCGATGCGTGTTTATTTTTC+AGG - chr4_2:74302454-74302473 Msa0621970:CDS 40.0%
CAGGAACTAGAGATTCCTTT+CGG - chr4_2:74302473-74302492 Msa0621970:CDS 40.0%
AAGAAAGGCCAAAACAGATC+AGG - chr4_2:74302529-74302548 Msa0621970:CDS 40.0%
ACACATCGATAGGAGAAGAA+GGG + chr4_2:74302555-74302574 None:intergenic 40.0%
CTCATTTCTCACACATCGAT+AGG + chr4_2:74302565-74302584 None:intergenic 40.0%
! ACTGAAGATGTTACCCCTTT+TGG - chr4_2:74302610-74302629 Msa0621970:intron 40.0%
ATTACAGCAAAGGCCAAAAG+GGG + chr4_2:74302626-74302645 None:intergenic 40.0%
! CATGTATCGGTGTAATGCAT+TGG - chr4_2:74302671-74302690 Msa0621970:intron 40.0%
AAGTCCAAAGGGTAGAATAG+CGG - chr4_2:74302837-74302856 Msa0621970:intron 40.0%
GAAAAACAACAGCCCAGTAA+AGG + chr4_2:74302897-74302916 None:intergenic 40.0%
ATGGATCGAGAATATAGAGC+AGG + chr4_2:74302990-74303009 None:intergenic 40.0%
!! TCTCGATCCATTTTGGTATG+AGG - chr4_2:74302999-74303018 Msa0621970:intron 40.0%
!! ACACTTGATAATGAGACAGC+CGG - chr4_2:74303091-74303110 Msa0621970:CDS 40.0%
!! TTGAATAATTTTGGGTGGGC+CGG + chr4_2:74303113-74303132 None:intergenic 40.0%
TCAATATGCATGTCGGAAGA+TGG - chr4_2:74303129-74303148 Msa0621970:CDS 40.0%
CAATATGCATGTCGGAAGAT+GGG - chr4_2:74303130-74303149 Msa0621970:CDS 40.0%
CGCATTCAATCAGTGAACAA+TGG - chr4_2:74303163-74303182 Msa0621970:exon 40.0%
CGCAGTAGTATCTCTTACAC+CGG - chr4_2:74300677-74300696 Msa0621970:three_prime_UTR 45.0%
GTATCTCTTACACCGGAATC+TGG - chr4_2:74300684-74300703 Msa0621970:three_prime_UTR 45.0%
TTACACCGGAATCTGGTCAT+TGG - chr4_2:74300691-74300710 Msa0621970:three_prime_UTR 45.0%
TACACCGGAATCTGGTCATT+GGG - chr4_2:74300692-74300711 Msa0621970:three_prime_UTR 45.0%
!!! CAGGCGTATTTTAGCAGATG+CGG + chr4_2:74300730-74300749 None:intergenic 45.0%
ATTAAGGTGGCAGAGTACAG+CGG + chr4_2:74300881-74300900 None:intergenic 45.0%
!!! AGCGATAACCGTTTTGGTTC+TGG + chr4_2:74300943-74300962 None:intergenic 45.0%
GATTGATCAGAAATGGAGGC+GGG - chr4_2:74301322-74301341 Msa0621970:exon 45.0%
CTAGGAAGAAATTGACGGCA+TGG + chr4_2:74301826-74301845 None:intergenic 45.0%
TAAATCAGTCGCTCTGACTG+AGG - chr4_2:74302091-74302110 Msa0621970:intron 45.0%
!!! GGAAGAGTGTGGGTTTTAAG+CGG - chr4_2:74302130-74302149 Msa0621970:intron 45.0%
ATTCCTTTCGGTTTCGACGA+AGG - chr4_2:74302485-74302504 Msa0621970:CDS 45.0%
TCGGATGCATACGAGAAGAA+AGG - chr4_2:74302514-74302533 Msa0621970:CDS 45.0%
! AAGAAGGGCCTGATCTGTTT+TGG + chr4_2:74302540-74302559 None:intergenic 45.0%
CACACATCGATAGGAGAAGA+AGG + chr4_2:74302556-74302575 None:intergenic 45.0%
! CCACCGCATTTTATCTTCTC+CGG - chr4_2:74302585-74302604 Msa0621970:intron 45.0%
GGGGTAACATCTTCAGTTTC+CGG + chr4_2:74302607-74302626 None:intergenic 45.0%
TACACCGCTATTCTACCCTT+TGG + chr4_2:74302844-74302863 None:intergenic 45.0%
CGATCCCAATGACCAGATTC+CGG + chr4_2:74300699-74300718 None:intergenic 50.0%
AGAAGGCCCATGAATGGATC+AGG + chr4_2:74300749-74300768 None:intergenic 50.0%
TACCCTTGCCAGAGCTAGAT+CGG + chr4_2:74300856-74300875 None:intergenic 50.0%
ATCGTACGGTGAGGAATCGT+CGG - chr4_2:74301354-74301373 Msa0621970:CDS 50.0%
ATCATGATCGTGAAACCGCC+AGG - chr4_2:74301403-74301422 Msa0621970:CDS 50.0%
TCATGATCGTGAAACCGCCA+GGG - chr4_2:74301404-74301423 Msa0621970:CDS 50.0%
AGACACCGGAGTAGTAGAAC+CGG + chr4_2:74301461-74301480 None:intergenic 50.0%
TTACCGGAAAACGGAGACAC+CGG + chr4_2:74301475-74301494 None:intergenic 50.0%
TGAGGTTTGAACCTCGACCT+TGG - chr4_2:74302109-74302128 Msa0621970:intron 50.0%
CTTTCGGTTTCGACGAAGGT+CGG - chr4_2:74302489-74302508 Msa0621970:CDS 50.0%
CCGGAGAAGATAAAATGCGG+TGG + chr4_2:74302588-74302607 None:intergenic 50.0%
TTTGTGCTGCGGTTCACGTT+AGG - chr4_2:74302706-74302725 Msa0621970:intron 50.0%
TCTTCATGGGATGGCTACAG+AGG - chr4_2:74303063-74303082 Msa0621970:CDS 50.0%
TGGTCGAGAAGGCCCATGAA+TGG + chr4_2:74300755-74300774 None:intergenic 55.0%
AGAACGGTCTCGGCAAGCTT+CGG - chr4_2:74300810-74300829 Msa0621970:three_prime_UTR 55.0%
ACCCTTGCCAGAGCTAGATC+GGG + chr4_2:74300855-74300874 None:intergenic 55.0%
AAGCGTGAGATCGTACGGTG+AGG - chr4_2:74301345-74301364 Msa0621970:CDS 55.0%
AAACCGCCAGGGTACGAAAG+TGG - chr4_2:74301415-74301434 Msa0621970:CDS 55.0%
GATCCACTTTCGTACCCTGG+CGG + chr4_2:74301421-74301440 None:intergenic 55.0%
GTACGAAAGTGGATCGCCTC+TGG - chr4_2:74301426-74301445 Msa0621970:CDS 55.0%
! ACTCCGGTGTCTCCGTTTTC+CGG - chr4_2:74301469-74301488 Msa0621970:intron 55.0%
! GGTGTCTCCGTTTTCCGGTA+AGG - chr4_2:74301474-74301493 Msa0621970:intron 55.0%
ACCTCGACCTTGGAAGAGTG+TGG - chr4_2:74302119-74302138 Msa0621970:intron 55.0%
! CCTCGACCTTGGAAGAGTGT+GGG - chr4_2:74302120-74302139 Msa0621970:intron 55.0%
CGACCTTCGTCGAAACCGAA+AGG + chr4_2:74302491-74302510 None:intergenic 55.0%
GTTTCGACGAAGGTCGGCAT+CGG - chr4_2:74302495-74302514 Msa0621970:CDS 55.0%
GTTAGGCATGGCGCTCTGTT+TGG - chr4_2:74302723-74302742 Msa0621970:intron 55.0%
TGGGCCTTCTCGACCAGTTG+TGG - chr4_2:74300759-74300778 Msa0621970:three_prime_UTR 60.0%
GGGCCTTCTCGACCAGTTGT+GGG - chr4_2:74300760-74300779 Msa0621970:three_prime_UTR 60.0%
CGTCCCACAACTGGTCGAGA+AGG + chr4_2:74300766-74300785 None:intergenic 60.0%
AGCTTGCCGAGACCGTTCTC+CGG + chr4_2:74300809-74300828 None:intergenic 60.0%
CTTGCCAGAGCTAGATCGGG+CGG + chr4_2:74300852-74300871 None:intergenic 60.0%
!! GCCCGATCTAGCTCTGGCAA+GGG - chr4_2:74300851-74300870 Msa0621970:three_prime_UTR 60.0%
! GCGGGAAGCGTGAGATCGTA+CGG - chr4_2:74301340-74301359 Msa0621970:CDS 60.0%
GGCGATCCACTTTCGTACCC+TGG + chr4_2:74301424-74301443 None:intergenic 60.0%
!! TCCCGCCGGTTCTACTACTC+CGG - chr4_2:74301453-74301472 Msa0621970:intron 60.0%
ACCGGAGTAGTAGAACCGGC+GGG + chr4_2:74301457-74301476 None:intergenic 60.0%
CACCGGAGTAGTAGAACCGG+CGG + chr4_2:74301458-74301477 None:intergenic 60.0%
CCCACACTCTTCCAAGGTCG+AGG + chr4_2:74302123-74302142 None:intergenic 60.0%
GCTGCGGTTCACGTTAGGCA+TGG - chr4_2:74302711-74302730 Msa0621970:intron 60.0%
!! CGACGGTGTCGTCCCACAAC+TGG + chr4_2:74300775-74300794 None:intergenic 65.0%
TTGTGGGACGACACCGTCGC+CGG - chr4_2:74300776-74300795 Msa0621970:three_prime_UTR 65.0%
AGAGCTAGATCGGGCGGCGA+AGG + chr4_2:74300846-74300865 None:intergenic 65.0%
! TTCGCCGCCCGATCTAGCTC+TGG - chr4_2:74300845-74300864 Msa0621970:three_prime_UTR 65.0%
!! CGCCCGATCTAGCTCTGGCA+AGG - chr4_2:74300850-74300869 Msa0621970:three_prime_UTR 65.0%
CGAGACCGTTCTCCGGCCGA+GGG + chr4_2:74300802-74300821 None:intergenic 70.0%
CCTCGGCCGGAGAACGGTCT+CGG - chr4_2:74300800-74300819 Msa0621970:three_prime_UTR 70.0%
! TCGCCTCTGGCTTCTCCCGC+CGG - chr4_2:74301439-74301458 Msa0621970:intron 70.0%
GAACCGGCGGGAGAAGCCAG+AGG + chr4_2:74301445-74301464 None:intergenic 70.0%
ACGACACCGTCGCCGGCCCT+CGG - chr4_2:74300783-74300802 Msa0621970:three_prime_UTR 75.0%
ACCGTTCTCCGGCCGAGGGC+CGG + chr4_2:74300798-74300817 None:intergenic 75.0%
CCGAGACCGTTCTCCGGCCG+AGG + chr4_2:74300803-74300822 None:intergenic 75.0%
! GCCGGCCCTCGGCCGGAGAA+CGG - chr4_2:74300794-74300813 Msa0621970:three_prime_UTR 80.0%
! CACCGTCGCCGGCCCTCGGC+CGG - chr4_2:74300787-74300806 Msa0621970:three_prime_UTR 85.0%
! CTCCGGCCGAGGGCCGGCGA+CGG + chr4_2:74300792-74300811 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 74300671 74303252 74300671 ID=Msa0621970;Name=Msa0621970
chr4_2 mRNA 74300671 74303252 74300671 ID=Msa0621970-mRNA-1;Parent=Msa0621970;Name=Msa0621970-mRNA-1;_AED=0.03;_eAED=0.03;_QI=87|0.5|0.66|1|0.5|0.66|3|669|128
chr4_2 exon 74300671 74301447 74300671 ID=Msa0621970-mRNA-1:exon:22396;Parent=Msa0621970-mRNA-1
chr4_2 exon 74302428 74302591 74302428 ID=Msa0621970-mRNA-1:exon:22395;Parent=Msa0621970-mRNA-1
chr4_2 exon 74303051 74303252 74303051 ID=Msa0621970-mRNA-1:exon:22394;Parent=Msa0621970-mRNA-1
chr4_2 five_prime_UTR 74303166 74303252 74303166 ID=Msa0621970-mRNA-1:five_prime_utr;Parent=Msa0621970-mRNA-1
chr4_2 CDS 74303051 74303165 74303051 ID=Msa0621970-mRNA-1:cds;Parent=Msa0621970-mRNA-1
chr4_2 CDS 74302428 74302591 74302428 ID=Msa0621970-mRNA-1:cds;Parent=Msa0621970-mRNA-1
chr4_2 CDS 74301340 74301447 74301340 ID=Msa0621970-mRNA-1:cds;Parent=Msa0621970-mRNA-1
chr4_2 three_prime_UTR 74300671 74301339 74300671 ID=Msa0621970-mRNA-1:three_prime_utr;Parent=Msa0621970-mRNA-1
Gene Sequence

>Msa0621970

ATGGGCCTTCTCGACCAGTTGTGGGACGACACCGTCGCCGGCCCTCGGCCGGAGAACGGTCTCGGCAAGCTTCGGAAGCAAAACACCTTCGCCGCCCGATCTAGCTCTGGCAAGGAAATGGAGGCGGGAAGCGTGAGATCGTACGGTGAGGAATCGTCGGAGCAAACGACGAGAGTAACACGTAGCATCATGATCGTGAAACCGCCAGGGTACGAAAGTGGATCGCCTCTGGCTTCTCCCGCCGGTTCTACTACTCCGGTGTCTCCGTTTTCCGGTAAGGAACTAGAGATTCCTTTCGGTTTCGACGAAGGTCGGCATCGGATGCATACGAGAAGAAAGGCCAAAACAGATCAGGCCCTTCTTCTCCTATCGATGTGTGAGAAATGA
Protein sequence

>Msa0621970

MGLLDQLWDDTVAGPRPENGLGKLRKQNTFAARSSSGKEMEAGSVRSYGEESSEQTTRVTRSIMIVKPPGYESGSPLASPAGSTTPVSPFSGKELEIPFGFDEGRHRMHTRRKAKTDQALLLLSMCEK*