AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1433330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1433330 MtrunA17_Chr7g0272351 95.652 92 4 0 1 92 1 92 1.60e-59 179
Msa1433330 MtrunA17_Chr1g0192251 66.990 103 30 3 1 101 1 101 5.52e-37 122
Msa1433330 MtrunA17_Chr8g0344711 39.316 117 53 5 1 117 1 99 6.37e-13 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1433330 AT1G56220.2 72.093 86 22 2 1 84 1 86 1.70e-36 121
Msa1433330 AT1G56220.4 72.093 86 22 2 1 84 26 111 2.05e-36 121
Msa1433330 AT1G56220.1 72.093 86 22 2 1 84 1 86 4.15e-36 120
Msa1433330 AT1G56220.3 72.093 86 22 2 1 84 26 111 4.48e-36 121
Msa1433330 AT1G56220.5 72.093 86 22 2 1 84 26 111 4.79e-36 121
Msa1433330 AT1G54070.1 47.761 67 21 2 1 67 1 53 1.90e-11 57.4

Find 96 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGCATATTGAATAATTT+TGG 0.110350 tig0027254:+52635 None:intergenic
TCTATATTCTCGATCCATTT+TGG 0.241117 tig0027254:-52762 Msa1433330:three_prime_UTR
ACTGAAGATGTTACCCCTTT+TGG 0.271295 tig0027254:-53145 Msa1433330:three_prime_UTR
GGGGTAACATCTTCAGTTTC+CGG 0.301859 tig0027254:+53151 None:intergenic
ACGAGAGATTGTCAAGAAAA+AGG 0.303375 tig0027254:+52804 None:intergenic
AAGAAGGGCCTGATCTGTTT+TGG 0.319411 tig0027254:+53218 None:intergenic
AATTCATCCTCATACCAAAA+TGG 0.338716 tig0027254:+52748 None:intergenic
AGTGAACAATGGAACAATAA+TGG 0.359458 tig0027254:-52580 Msa1433330:three_prime_UTR
GTTAGGCATGGCGCTCTGTT+TGG 0.361146 tig0027254:-53032 Msa1433330:three_prime_UTR
TGTGTATATAGATCCTTTAC+TGG 0.368772 tig0027254:-52873 Msa1433330:three_prime_UTR
AGTGTAATAAGTTGTAAGTA+AGG 0.373833 tig0027254:-52940 Msa1433330:three_prime_UTR
GTGAATAATAACGACCTTAC+CGG 0.378031 tig0027254:+54259 None:intergenic
TACACCGCTATTCTACCCTT+TGG 0.402296 tig0027254:+52913 None:intergenic
CGATCCCAATGACCAGATTC+CGG 0.418252 tig0027254:+55044 None:intergenic
TACCCTTGCCAGAGCTAGAT+CGG 0.420036 tig0027254:+54887 None:intergenic
CAGGAACTAGAGATTCCTTT+CGG 0.426315 tig0027254:-53282 Msa1433330:CDS
CCGAGACCGTTCTCCGGCCG+AGG 0.426718 tig0027254:+54940 None:intergenic
CGACGGTGTCGTCCCACAAC+TGG 0.429141 tig0027254:+54968 None:intergenic
ACCGTTCTCCGGCCGAGGGC+CGG 0.433178 tig0027254:+54945 None:intergenic
CTCCGGCCGAGGGCCGGCGA+CGG 0.445786 tig0027254:+54951 None:intergenic
CACCGTCGCCGGCCCTCGGC+CGG 0.447404 tig0027254:-54953 Msa1433330:CDS
GTATCTCTTACACCGGAATC+TGG 0.448206 tig0027254:-55056 Msa1433330:five_prime_UTR
AGAACGGTCTCGGCAAGCTT+CGG 0.450915 tig0027254:-54930 Msa1433330:CDS
TGGGCCTTCTCGACCAGTTG+TGG 0.456063 tig0027254:-54981 Msa1433330:CDS
TTTGTGCTGCGGTTCACGTT+AGG 0.456617 tig0027254:-53049 Msa1433330:three_prime_UTR
TACACCGGAATCTGGTCATT+GGG 0.458037 tig0027254:-55048 Msa1433330:five_prime_UTR
TCGCCTCTGGCTTCTCCCGC+CGG 0.458567 tig0027254:-54308 Msa1433330:CDS
AAATACGCCTGATCCATTCA+TGG 0.461198 tig0027254:-55001 Msa1433330:exon
TATTACAGCAAAGGCCAAAA+GGG 0.461978 tig0027254:+53131 None:intergenic
TTACACCGGAATCTGGTCAT+TGG 0.463228 tig0027254:-55049 Msa1433330:five_prime_UTR
ACGACACCGTCGCCGGCCCT+CGG 0.467035 tig0027254:-54957 Msa1433330:CDS
CCTCGGCCGGAGAACGGTCT+CGG 0.467575 tig0027254:-54940 Msa1433330:CDS
TCAATATGCATGTCGGAAGA+TGG 0.467631 tig0027254:-52625 Msa1433330:three_prime_UTR
TTATTACAGCAAAGGCCAAA+AGG 0.472228 tig0027254:+53130 None:intergenic
CGCATTCAATCAGTGAACAA+TGG 0.473374 tig0027254:-52591 Msa1433330:three_prime_UTR
CTTTCGGTTTCGACGAAGGT+CGG 0.476530 tig0027254:-53266 Msa1433330:CDS
GCCGGCCCTCGGCCGGAGAA+CGG 0.477433 tig0027254:-54946 Msa1433330:CDS
AGAAGGCCCATGAATGGATC+AGG 0.480654 tig0027254:+54994 None:intergenic
ATAACGACCTTACCGGAAAA+CGG 0.482174 tig0027254:+54266 None:intergenic
GTACGAAAGTGGATCGCCTC+TGG 0.484048 tig0027254:-54321 Msa1433330:CDS
CGCCCGATCTAGCTCTGGCA+AGG 0.484849 tig0027254:-54890 Msa1433330:CDS
AAGAAAGGCCAAAACAGATC+AGG 0.489997 tig0027254:-53226 Msa1433330:CDS
ATCATGATCGTGAAACCGCC+AGG 0.492141 tig0027254:-54344 Msa1433330:CDS
TTCGCCGCCCGATCTAGCTC+TGG 0.500632 tig0027254:-54895 Msa1433330:CDS
AACAATAATGGACATGAAGA+AGG 0.503571 tig0027254:-52568 Msa1433330:three_prime_UTR
GGGCCTTCTCGACCAGTTGT+GGG 0.507384 tig0027254:-54980 Msa1433330:CDS
AGAGCTAGATCGGGCGGCGA+AGG 0.508256 tig0027254:+54897 None:intergenic
TGTAAGTAAGGAAGTCCAAA+GGG 0.513200 tig0027254:-52928 Msa1433330:three_prime_UTR
CGCAGTAGTATCTCTTACAC+CGG 0.520795 tig0027254:-55063 Msa1433330:five_prime_UTR
TGGTCGAGAAGGCCCATGAA+TGG 0.522793 tig0027254:+54988 None:intergenic
GTTTCGACGAAGGTCGGCAT+CGG 0.527766 tig0027254:-53260 Msa1433330:CDS
CACACATCGATAGGAGAAGA+AGG 0.528061 tig0027254:+53202 None:intergenic
ATAATGGACATGAAGAAGGT+TGG 0.529729 tig0027254:-52564 Msa1433330:three_prime_UTR
ATTCCTTTCGGTTTCGACGA+AGG 0.530725 tig0027254:-53270 Msa1433330:CDS
TCCCGCCGGTTCTACTACTC+CGG 0.530743 tig0027254:-54294 Msa1433330:CDS
GTTTATTTGTGTGCATGTAT+CGG 0.533623 tig0027254:-53097 Msa1433330:three_prime_UTR
CAATATGCATGTCGGAAGAT+GGG 0.533830 tig0027254:-52624 Msa1433330:three_prime_UTR
ACCCTTGCCAGAGCTAGATC+GGG 0.537273 tig0027254:+54888 None:intergenic
AGCTTGCCGAGACCGTTCTC+CGG 0.541689 tig0027254:+54934 None:intergenic
GCTGCGGTTCACGTTAGGCA+TGG 0.543848 tig0027254:-53044 Msa1433330:three_prime_UTR
GCGGGAAGCGTGAGATCGTA+CGG 0.546978 tig0027254:-54407 Msa1433330:CDS
ATCGTACGGTGAGGAATCGT+CGG 0.548167 tig0027254:-54393 Msa1433330:CDS
AGACACCGGAGTAGTAGAAC+CGG 0.549756 tig0027254:+54289 None:intergenic
AAACCGCCAGGGTACGAAAG+TGG 0.556609 tig0027254:-54332 Msa1433330:CDS
GAACCGGCGGGAGAAGCCAG+AGG 0.561661 tig0027254:+54305 None:intergenic
CGAGACCGTTCTCCGGCCGA+GGG 0.565198 tig0027254:+54941 None:intergenic
AATACGCCTGATCCATTCAT+GGG 0.567709 tig0027254:-55000 Msa1433330:exon
GGCGATCCACTTTCGTACCC+TGG 0.568040 tig0027254:+54326 None:intergenic
CGACCTTCGTCGAAACCGAA+AGG 0.571902 tig0027254:+53267 None:intergenic
ATGGATCGAGAATATAGAGC+AGG 0.575307 tig0027254:+52767 None:intergenic
TCGGATGCATACGAGAAGAA+AGG 0.583908 tig0027254:-53241 Msa1433330:CDS
AAATTATTCAATATGCATGT+CGG 0.584174 tig0027254:-52632 Msa1433330:three_prime_UTR
GCCCGATCTAGCTCTGGCAA+GGG 0.585634 tig0027254:-54889 Msa1433330:intron
GATTGATCAGAAATGGAGGC+GGG 0.586985 tig0027254:-54425 Msa1433330:intron
CGTCCCACAACTGGTCGAGA+AGG 0.590163 tig0027254:+54977 None:intergenic
CATGTATCGGTGTAATGCAT+TGG 0.591207 tig0027254:-53084 Msa1433330:three_prime_UTR
TTGTGGGACGACACCGTCGC+CGG 0.591995 tig0027254:-54964 Msa1433330:CDS
ACACTTGATAATGAGACAGC+CGG 0.594836 tig0027254:-52663 Msa1433330:three_prime_UTR
TTGTAAGTAAGGAAGTCCAA+AGG 0.595220 tig0027254:-52929 Msa1433330:three_prime_UTR
CTCATTTCTCACACATCGAT+AGG 0.601241 tig0027254:+53193 None:intergenic
TGATTGATCAGAAATGGAGG+CGG 0.603270 tig0027254:-54426 Msa1433330:intron
GTGTATATAGATCCTTTACT+GGG 0.611023 tig0027254:-52872 Msa1433330:three_prime_UTR
TCTTCATGGGATGGCTACAG+AGG 0.611027 tig0027254:-52691 Msa1433330:three_prime_UTR
AATTGATTGATCAGAAATGG+AGG 0.629008 tig0027254:-54429 Msa1433330:intron
TTACCGGAAAACGGAGACAC+CGG 0.636278 tig0027254:+54275 None:intergenic
ACACATCGATAGGAGAAGAA+GGG 0.647952 tig0027254:+53203 None:intergenic
CACCGGAGTAGTAGAACCGG+CGG 0.650193 tig0027254:+54292 None:intergenic
ATTACAGCAAAGGCCAAAAG+GGG 0.651252 tig0027254:+53132 None:intergenic
TCATGATCGTGAAACCGCCA+GGG 0.653940 tig0027254:-54343 Msa1433330:CDS
CTTGCCAGAGCTAGATCGGG+CGG 0.658767 tig0027254:+54891 None:intergenic
ACCGGAGTAGTAGAACCGGC+GGG 0.676139 tig0027254:+54293 None:intergenic
CCGGAGAAGATAAAATGCGG+TGG 0.684216 tig0027254:+53170 None:intergenic
AAGTCCAAAGGGTAGAATAG+CGG 0.707284 tig0027254:-52917 Msa1433330:three_prime_UTR
GATCCACTTTCGTACCCTGG+CGG 0.709901 tig0027254:+54329 None:intergenic
AAGCGTGAGATCGTACGGTG+AGG 0.726983 tig0027254:-54402 Msa1433330:CDS
TTTCCGGAGAAGATAAAATG+CGG 0.732385 tig0027254:+53167 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATATTGTCATATATTTA+AGG - tig0027254:54065-54084 Msa1433330:intron 10.0%
!! ATCATTTATAATAACAATAA+TGG - tig0027254:54145-54164 Msa1433330:intron 10.0%
!! AAAAAAAAAAACTTATTACA+CGG + tig0027254:54621-54640 None:intergenic 10.0%
!! AAATTATATACGAGAAATTA+AGG + tig0027254:52750-52769 None:intergenic 15.0%
!! AAAATTTAAAAGCAAAAGTT+AGG + tig0027254:53492-53511 None:intergenic 15.0%
!! TTGAAGGAATAATTAATATT+AGG - tig0027254:53535-53554 Msa1433330:intron 15.0%
!! AACCAACTTATTAATATTAA+GGG + tig0027254:53833-53852 None:intergenic 15.0%
!! TAACCAACTTATTAATATTA+AGG + tig0027254:53834-53853 None:intergenic 15.0%
!!! TTTATAATAACAATAATGGA+AGG - tig0027254:54149-54168 Msa1433330:intron 15.0%
!! TGAAGGTTAATCAATATTAA+CGG + tig0027254:52871-52890 None:intergenic 20.0%
!!! AAGCTTAAAGTTTGAAATTA+TGG - tig0027254:52946-52965 Msa1433330:three_prime_UTR 20.0%
!! ATTAGGCTTAAATATGTTAT+TGG - tig0027254:53552-53571 Msa1433330:intron 20.0%
!! GAAACATTTATTGAAATGTA+AGG - tig0027254:53761-53780 Msa1433330:intron 20.0%
!!! TTAAGGGTTAAATATGTTTT+TGG + tig0027254:53817-53836 None:intergenic 20.0%
!! AACCCTTAATATTAATAAGT+TGG - tig0027254:53828-53847 Msa1433330:intron 20.0%
!! GAAAAAAATTATTACAGCAA+AGG + tig0027254:54474-54493 None:intergenic 20.0%
!!! AGATTCTTTTAAGTCTTATT+AGG - tig0027254:54591-54610 Msa1433330:intron 20.0%
!!! CATGCATATTGAATAATTTT+GGG + tig0027254:54960-54979 None:intergenic 20.0%
!!! ACATGCATATTGAATAATTT+TGG + tig0027254:54961-54980 None:intergenic 20.0%
!! AAATTATTCAATATGCATGT+CGG - tig0027254:54961-54980 Msa1433330:CDS 20.0%
! TTATATACGAGAAATTAAGG+TGG + tig0027254:52747-52766 None:intergenic 25.0%
! CAATTCGATATTCAATTTGA+AGG + tig0027254:52888-52907 None:intergenic 25.0%
!!! AAGTCTTGCATGTTTTATTT+TGG - tig0027254:53014-53033 Msa1433330:three_prime_UTR 25.0%
! AGAAATTGATTGATCAGAAA+TGG - tig0027254:53161-53180 Msa1433330:three_prime_UTR 25.0%
! TTTACTTCACTTTACTTTAC+TGG + tig0027254:53420-53439 None:intergenic 25.0%
! GTAAATGATCTCCTAAATTT+CGG - tig0027254:53435-53454 Msa1433330:intron 25.0%
! TAAATGATCTCCTAAATTTC+GGG - tig0027254:53436-53455 Msa1433330:intron 25.0%
!! TGCTATATTTTGTCTTTTTG+TGG - tig0027254:53461-53480 Msa1433330:intron 25.0%
!! TATTTTGTCTTTTTGTGGAA+TGG - tig0027254:53466-53485 Msa1433330:intron 25.0%
!! ATACTACGGTTTCTAAATTT+TGG + tig0027254:53692-53711 None:intergenic 25.0%
! CAAAACTTCAACAAATACTA+CGG + tig0027254:53706-53725 None:intergenic 25.0%
! ATTCAAAGTCGTGTAAAATA+TGG - tig0027254:53790-53809 Msa1433330:intron 25.0%
!!! GTGCTATTTTATTAATTGTG+TGG - tig0027254:54005-54024 Msa1433330:intron 25.0%
!!! CTAGTTGTCTATTTTAACTT+GGG + tig0027254:54101-54120 None:intergenic 25.0%
! AGTGTAATAAGTTGTAAGTA+AGG - tig0027254:54653-54672 Msa1433330:intron 25.0%
!!! CATATTGAATAATTTTGGGT+GGG + tig0027254:54956-54975 None:intergenic 25.0%
!! AATTTTAGCGATAACCGTTT+TGG + tig0027254:52802-52821 None:intergenic 30.0%
TGAATATCGAATTGTGTTCT+AGG - tig0027254:52894-52913 Msa1433330:three_prime_UTR 30.0%
AATTGATTGATCAGAAATGG+AGG - tig0027254:53164-53183 Msa1433330:three_prime_UTR 30.0%
!!! CACTTTACTTTACTGGTTTT+TGG + tig0027254:53413-53432 None:intergenic 30.0%
!! GTTTCTAAATTTTGGTGACT+AGG + tig0027254:53684-53703 None:intergenic 30.0%
TTGTTCAACTTGAACAAAAC+CGG - tig0027254:53723-53742 Msa1433330:intron 30.0%
!! TTTCTAAAATCCCTACATTC+CGG + tig0027254:53745-53764 None:intergenic 30.0%
!!! CCTAGTTGTCTATTTTAACT+TGG + tig0027254:54102-54121 None:intergenic 30.0%
CCAAGTTAAAATAGACAACT+AGG - tig0027254:54099-54118 Msa1433330:intron 30.0%
GTTTATTTGTGTGCATGTAT+CGG - tig0027254:54496-54515 Msa1433330:intron 30.0%
TGTGTATATAGATCCTTTAC+TGG - tig0027254:54720-54739 Msa1433330:intron 30.0%
GTGTATATAGATCCTTTACT+GGG - tig0027254:54721-54740 Msa1433330:intron 30.0%
! TCTATATTCTCGATCCATTT+TGG - tig0027254:54831-54850 Msa1433330:intron 30.0%
AATTCATCCTCATACCAAAA+TGG + tig0027254:54848-54867 None:intergenic 30.0%
! TGGAACATTTTTGTCTTCAT+GGG - tig0027254:54889-54908 Msa1433330:intron 30.0%
! GCATATTGAATAATTTTGGG+TGG + tig0027254:54957-54976 None:intergenic 30.0%
AGTGAACAATGGAACAATAA+TGG - tig0027254:55013-55032 Msa1433330:five_prime_UTR 30.0%
!! AACAATAATGGACATGAAGA+AGG - tig0027254:55025-55044 Msa1433330:five_prime_UTR 30.0%
GTGATTCGTTTAACGAACAT+CGG + tig0027254:52774-52793 None:intergenic 35.0%
GTGAATAATAACGACCTTAC+CGG + tig0027254:53337-53356 None:intergenic 35.0%
! AGTTTTACCGTCAAGAAACA+CGG + tig0027254:53375-53394 None:intergenic 35.0%
!!! ACTTTACTGGTTTTTGGAAC+AGG + tig0027254:53407-53426 None:intergenic 35.0%
ATATAGCATGCCCGAAATTT+AGG + tig0027254:53449-53468 None:intergenic 35.0%
TTGAACAAAACCGGAATGTA+GGG - tig0027254:53732-53751 Msa1433330:intron 35.0%
GACAACTAGGTTAAGTTACT+TGG - tig0027254:54112-54131 Msa1433330:intron 35.0%
TTTCCGGAGAAGATAAAATG+CGG + tig0027254:54429-54448 None:intergenic 35.0%
TATTACAGCAAAGGCCAAAA+GGG + tig0027254:54465-54484 None:intergenic 35.0%
TTATTACAGCAAAGGCCAAA+AGG + tig0027254:54466-54485 None:intergenic 35.0%
!! TGAATTGAAGTTTTGTGCTG+CGG - tig0027254:54533-54552 Msa1433330:intron 35.0%
TTGTAAGTAAGGAAGTCCAA+AGG - tig0027254:54664-54683 Msa1433330:intron 35.0%
TGTAAGTAAGGAAGTCCAAA+GGG - tig0027254:54665-54684 Msa1433330:intron 35.0%
ACGAGAGATTGTCAAGAAAA+AGG + tig0027254:54792-54811 None:intergenic 35.0%
!!! AAGTCCAAGAAGTGTTTTTG+TGG - tig0027254:54869-54888 Msa1433330:intron 35.0%
TGTTCCACAAAAACACTTCT+TGG + tig0027254:54876-54895 None:intergenic 35.0%
! GTGGAACATTTTTGTCTTCA+TGG - tig0027254:54888-54907 Msa1433330:intron 35.0%
! ACATTTTTGTCTTCATGGGA+TGG - tig0027254:54893-54912 Msa1433330:CDS 35.0%
!! ATAATGGACATGAAGAAGGT+TGG - tig0027254:55029-55048 Msa1433330:five_prime_UTR 35.0%
AAATACGCCTGATCCATTCA+TGG - tig0027254:52592-52611 Msa1433330:three_prime_UTR 40.0%
AATACGCCTGATCCATTCAT+GGG - tig0027254:52593-52612 Msa1433330:three_prime_UTR 40.0%
ACGAATCACCAGAACCAAAA+CGG - tig0027254:52785-52804 Msa1433330:three_prime_UTR 40.0%
! TCTTTGAGCTTTTCACATGC+AGG + tig0027254:53051-53070 None:intergenic 40.0%
TGATTGATCAGAAATGGAGG+CGG - tig0027254:53167-53186 Msa1433330:three_prime_UTR 40.0%
ATAACGACCTTACCGGAAAA+CGG + tig0027254:53330-53349 None:intergenic 40.0%
CTTATACCCGTGTTTCTTGA+CGG - tig0027254:53365-53384 Msa1433330:intron 40.0%
! GTTTTACCGTCAAGAAACAC+GGG + tig0027254:53374-53393 None:intergenic 40.0%
TCAAAGTCGCGCAAAATGTA+AGG + tig0027254:53579-53598 None:intergenic 40.0%
GGTGACTAGGAAGAAATTGA+CGG + tig0027254:53671-53690 None:intergenic 40.0%
CTTGAACAAAACCGGAATGT+AGG - tig0027254:53731-53750 Msa1433330:intron 40.0%
!! GTAAGTTGATACGAGACTTG+TGG + tig0027254:53861-53880 None:intergenic 40.0%
GTCTCGTATCAACTTACAAG+CGG - tig0027254:53864-53883 Msa1433330:intron 40.0%
TTAAAACCCACACTCTTCCA+AGG + tig0027254:53967-53986 None:intergenic 40.0%
!! GCGATGCGTGTTTATTTTTC+AGG - tig0027254:54292-54311 Msa1433330:CDS 40.0%
CAGGAACTAGAGATTCCTTT+CGG - tig0027254:54311-54330 Msa1433330:CDS 40.0%
AAGAAAGGCCAAAACAGATC+AGG - tig0027254:54367-54386 Msa1433330:CDS 40.0%
ACACATCGATAGGAGAAGAA+GGG + tig0027254:54393-54412 None:intergenic 40.0%
CTCATTTCTCACACATCGAT+AGG + tig0027254:54403-54422 None:intergenic 40.0%
! ACTGAAGATGTTACCCCTTT+TGG - tig0027254:54448-54467 Msa1433330:intron 40.0%
ATTACAGCAAAGGCCAAAAG+GGG + tig0027254:54464-54483 None:intergenic 40.0%
! CATGTATCGGTGTAATGCAT+TGG - tig0027254:54509-54528 Msa1433330:intron 40.0%
AAGTCCAAAGGGTAGAATAG+CGG - tig0027254:54676-54695 Msa1433330:intron 40.0%
GAAAAACAACAGCCCAGTAA+AGG + tig0027254:54736-54755 None:intergenic 40.0%
ATGGATCGAGAATATAGAGC+AGG + tig0027254:54829-54848 None:intergenic 40.0%
!! TCTCGATCCATTTTGGTATG+AGG - tig0027254:54838-54857 Msa1433330:intron 40.0%
!! ACACTTGATAATGAGACAGC+CGG - tig0027254:54930-54949 Msa1433330:CDS 40.0%
!! TTGAATAATTTTGGGTGGGC+CGG + tig0027254:54952-54971 None:intergenic 40.0%
TCAATATGCATGTCGGAAGA+TGG - tig0027254:54968-54987 Msa1433330:CDS 40.0%
CAATATGCATGTCGGAAGAT+GGG - tig0027254:54969-54988 Msa1433330:CDS 40.0%
CGCATTCAATCAGTGAACAA+TGG - tig0027254:55002-55021 Msa1433330:exon 40.0%
CGCAGTAGTATCTCTTACAC+CGG - tig0027254:52530-52549 Msa1433330:three_prime_UTR 45.0%
GTATCTCTTACACCGGAATC+TGG - tig0027254:52537-52556 Msa1433330:three_prime_UTR 45.0%
TTACACCGGAATCTGGTCAT+TGG - tig0027254:52544-52563 Msa1433330:three_prime_UTR 45.0%
TACACCGGAATCTGGTCATT+GGG - tig0027254:52545-52564 Msa1433330:three_prime_UTR 45.0%
!!! CAGGCGTATTTTAGCAGATG+CGG + tig0027254:52583-52602 None:intergenic 45.0%
ATTAAGGTGGCAGAGTACAG+CGG + tig0027254:52734-52753 None:intergenic 45.0%
!!! AGCGATAACCGTTTTGGTTC+TGG + tig0027254:52796-52815 None:intergenic 45.0%
GATTGATCAGAAATGGAGGC+GGG - tig0027254:53168-53187 Msa1433330:three_prime_UTR 45.0%
ACTAGGAAGAAATTGACGGC+AGG + tig0027254:53667-53686 None:intergenic 45.0%
TAAATCAGTCGCTCTGACTG+AGG - tig0027254:53929-53948 Msa1433330:intron 45.0%
!!! GGAAGAGTGTGGGTTTTAAG+CGG - tig0027254:53968-53987 Msa1433330:intron 45.0%
ATTCCTTTCGGTTTCGACGA+AGG - tig0027254:54323-54342 Msa1433330:CDS 45.0%
TCGGATGCATACGAGAAGAA+AGG - tig0027254:54352-54371 Msa1433330:CDS 45.0%
! AAGAAGGGCCTGATCTGTTT+TGG + tig0027254:54378-54397 None:intergenic 45.0%
CACACATCGATAGGAGAAGA+AGG + tig0027254:54394-54413 None:intergenic 45.0%
! CCACCGCATTTTATCTTCTC+CGG - tig0027254:54423-54442 Msa1433330:intron 45.0%
GGGGTAACATCTTCAGTTTC+CGG + tig0027254:54445-54464 None:intergenic 45.0%
TACACCGCTATTCTACCCTT+TGG + tig0027254:54683-54702 None:intergenic 45.0%
!!! ATATTAATATTTTTTTTTGA+AGG - tig0027254:53519-53538 Msa1433330:intron 5.0%
!!! TTTTTTTTTTTTTTTTTTGA+AGG - tig0027254:54630-54649 Msa1433330:intron 5.0%
CGATCCCAATGACCAGATTC+CGG + tig0027254:52552-52571 None:intergenic 50.0%
AGAAGGCCCATGAATGGATC+AGG + tig0027254:52602-52621 None:intergenic 50.0%
TACCCTTGCCAGAGCTAGAT+CGG + tig0027254:52709-52728 None:intergenic 50.0%
ATCGTACGGTGAGGAATCGT+CGG - tig0027254:53200-53219 Msa1433330:CDS 50.0%
ATCATGATCGTGAAACCGCC+AGG - tig0027254:53249-53268 Msa1433330:CDS 50.0%
TCATGATCGTGAAACCGCCA+GGG - tig0027254:53250-53269 Msa1433330:CDS 50.0%
AGACACCGGAGTAGTAGAAC+CGG + tig0027254:53307-53326 None:intergenic 50.0%
TTACCGGAAAACGGAGACAC+CGG + tig0027254:53321-53340 None:intergenic 50.0%
TGAGGTTTGAACCTCGACCT+TGG - tig0027254:53947-53966 Msa1433330:intron 50.0%
CTTTCGGTTTCGACGAAGGT+CGG - tig0027254:54327-54346 Msa1433330:CDS 50.0%
CCGGAGAAGATAAAATGCGG+TGG + tig0027254:54426-54445 None:intergenic 50.0%
TTTGTGCTGCGGTTCACGTT+AGG - tig0027254:54544-54563 Msa1433330:intron 50.0%
TCTTCATGGGATGGCTACAG+AGG - tig0027254:54902-54921 Msa1433330:CDS 50.0%
TGGTCGAGAAGGCCCATGAA+TGG + tig0027254:52608-52627 None:intergenic 55.0%
AGAACGGTCTCGGCAAGCTT+CGG - tig0027254:52663-52682 Msa1433330:three_prime_UTR 55.0%
ACCCTTGCCAGAGCTAGATC+GGG + tig0027254:52708-52727 None:intergenic 55.0%
AAGCGTGAGATCGTACGGTG+AGG - tig0027254:53191-53210 Msa1433330:exon 55.0%
AAACCGCCAGGGTACGAAAG+TGG - tig0027254:53261-53280 Msa1433330:CDS 55.0%
GATCCACTTTCGTACCCTGG+CGG + tig0027254:53267-53286 None:intergenic 55.0%
GTACGAAAGTGGATCGCCTC+TGG - tig0027254:53272-53291 Msa1433330:CDS 55.0%
! ACTCCGGTGTCTCCGTTTTC+CGG - tig0027254:53315-53334 Msa1433330:intron 55.0%
! GGTGTCTCCGTTTTCCGGTA+AGG - tig0027254:53320-53339 Msa1433330:intron 55.0%
ACCTCGACCTTGGAAGAGTG+TGG - tig0027254:53957-53976 Msa1433330:intron 55.0%
! CCTCGACCTTGGAAGAGTGT+GGG - tig0027254:53958-53977 Msa1433330:intron 55.0%
CGACCTTCGTCGAAACCGAA+AGG + tig0027254:54329-54348 None:intergenic 55.0%
GTTTCGACGAAGGTCGGCAT+CGG - tig0027254:54333-54352 Msa1433330:CDS 55.0%
GTTAGGCATGGCGCTCTGTT+TGG - tig0027254:54561-54580 Msa1433330:intron 55.0%
TGGGCCTTCTCGACCAGTTG+TGG - tig0027254:52612-52631 Msa1433330:three_prime_UTR 60.0%
GGGCCTTCTCGACCAGTTGT+GGG - tig0027254:52613-52632 Msa1433330:three_prime_UTR 60.0%
CGTCCCACAACTGGTCGAGA+AGG + tig0027254:52619-52638 None:intergenic 60.0%
AGCTTGCCGAGACCGTTCTC+CGG + tig0027254:52662-52681 None:intergenic 60.0%
CTTGCCAGAGCTAGATCGGG+CGG + tig0027254:52705-52724 None:intergenic 60.0%
!! GCCCGATCTAGCTCTGGCAA+GGG - tig0027254:52704-52723 Msa1433330:three_prime_UTR 60.0%
! GCGGGAAGCGTGAGATCGTA+CGG - tig0027254:53186-53205 Msa1433330:exon 60.0%
GGCGATCCACTTTCGTACCC+TGG + tig0027254:53270-53289 None:intergenic 60.0%
!! TCCCGCCGGTTCTACTACTC+CGG - tig0027254:53299-53318 Msa1433330:intron 60.0%
ACCGGAGTAGTAGAACCGGC+GGG + tig0027254:53303-53322 None:intergenic 60.0%
CACCGGAGTAGTAGAACCGG+CGG + tig0027254:53304-53323 None:intergenic 60.0%
CCCACACTCTTCCAAGGTCG+AGG + tig0027254:53961-53980 None:intergenic 60.0%
GCTGCGGTTCACGTTAGGCA+TGG - tig0027254:54549-54568 Msa1433330:intron 60.0%
!! CGACGGTGTCGTCCCACAAC+TGG + tig0027254:52628-52647 None:intergenic 65.0%
TTGTGGGACGACACCGTCGC+CGG - tig0027254:52629-52648 Msa1433330:three_prime_UTR 65.0%
AGAGCTAGATCGGGCGGCGA+AGG + tig0027254:52699-52718 None:intergenic 65.0%
! TTCGCCGCCCGATCTAGCTC+TGG - tig0027254:52698-52717 Msa1433330:three_prime_UTR 65.0%
!! CGCCCGATCTAGCTCTGGCA+AGG - tig0027254:52703-52722 Msa1433330:three_prime_UTR 65.0%
CGAGACCGTTCTCCGGCCGA+GGG + tig0027254:52655-52674 None:intergenic 70.0%
CCTCGGCCGGAGAACGGTCT+CGG - tig0027254:52653-52672 Msa1433330:three_prime_UTR 70.0%
! TCGCCTCTGGCTTCTCCCGC+CGG - tig0027254:53285-53304 Msa1433330:intron 70.0%
GAACCGGCGGGAGAAGCCAG+AGG + tig0027254:53291-53310 None:intergenic 70.0%
ACGACACCGTCGCCGGCCCT+CGG - tig0027254:52636-52655 Msa1433330:three_prime_UTR 75.0%
ACCGTTCTCCGGCCGAGGGC+CGG + tig0027254:52651-52670 None:intergenic 75.0%
CCGAGACCGTTCTCCGGCCG+AGG + tig0027254:52656-52675 None:intergenic 75.0%
! GCCGGCCCTCGGCCGGAGAA+CGG - tig0027254:52647-52666 Msa1433330:three_prime_UTR 80.0%
! CACCGTCGCCGGCCCTCGGC+CGG - tig0027254:52640-52659 Msa1433330:three_prime_UTR 85.0%
! CTCCGGCCGAGGGCCGGCGA+CGG + tig0027254:52645-52664 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
tig0027254 gene 52524 55091 52524 ID=Msa1433330;Name=Msa1433330
tig0027254 mRNA 52524 55091 52524 ID=Msa1433330-mRNA-1;Parent=Msa1433330;Name=Msa1433330-mRNA-1;_AED=0.02;_eAED=0.02;_QI=87|0.5|0.66|1|0.5|0.66|3|670|128
tig0027254 exon 52524 53301 52524 ID=Msa1433330-mRNA-1:exon:8579;Parent=Msa1433330-mRNA-1
tig0027254 exon 54274 54437 54274 ID=Msa1433330-mRNA-1:exon:8578;Parent=Msa1433330-mRNA-1
tig0027254 exon 54890 55091 54890 ID=Msa1433330-mRNA-1:exon:8577;Parent=Msa1433330-mRNA-1
tig0027254 five_prime_UTR 55005 55091 55005 ID=Msa1433330-mRNA-1:five_prime_utr;Parent=Msa1433330-mRNA-1
tig0027254 CDS 54890 55004 54890 ID=Msa1433330-mRNA-1:cds;Parent=Msa1433330-mRNA-1
tig0027254 CDS 54274 54437 54274 ID=Msa1433330-mRNA-1:cds;Parent=Msa1433330-mRNA-1
tig0027254 CDS 53194 53301 53194 ID=Msa1433330-mRNA-1:cds;Parent=Msa1433330-mRNA-1
tig0027254 three_prime_UTR 52524 53193 52524 ID=Msa1433330-mRNA-1:three_prime_utr;Parent=Msa1433330-mRNA-1
Gene Sequence

>Msa1433330

ATGGGCCTTCTCGACCAGTTGTGGGACGACACCGTCGCCGGCCCTCGGCCGGAGAACGGTCTCGGCAAGCTTCGGAAGCAAAACACCTTCGCCGCCCGATCTAGCTCTGGCAAGGAAATGGAGGCGGGAAGCGTGAGATCGTACGGTGAGGAATCGTCGGAGCAAACGACGAGAGTAACACGTAGCATCATGATCGTGAAACCGCCAGGGTACGAAAGTGGATCGCCTCTGGCTTCTCCCGCCGGTTCTACTACTCCGGTGTCTCCGTTTTCCGGTAAGGAACTAGAGATTCCTTTCGGTTTCGACGAAGGTCGGCATCGGATGCATACGAGAAGAAAGGCCAAAACAGATCAGGCCCTTCTTCTCCTATCGATGTGTGAGAAATGA
Protein sequence

>Msa1433330

MGLLDQLWDDTVAGPRPENGLGKLRKQNTFAARSSSGKEMEAGSVRSYGEESSEQTTRVTRSIMIVKPPGYESGSPLASPAGSTTPVSPFSGKELEIPFGFDEGRHRMHTRRKAKTDQALLLLSMCEK*