AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1305070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1305070 MtrunA17_Chr7g0272351 95.652 92 4 0 1 92 1 92 1.60e-59 179
Msa1305070 MtrunA17_Chr1g0192251 66.990 103 30 3 1 101 1 101 5.52e-37 122
Msa1305070 MtrunA17_Chr8g0344711 39.316 117 53 5 1 117 1 99 6.37e-13 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1305070 AT1G56220.2 72.093 86 22 2 1 84 1 86 1.70e-36 121
Msa1305070 AT1G56220.4 72.093 86 22 2 1 84 26 111 2.05e-36 121
Msa1305070 AT1G56220.1 72.093 86 22 2 1 84 1 86 4.15e-36 120
Msa1305070 AT1G56220.3 72.093 86 22 2 1 84 26 111 4.48e-36 121
Msa1305070 AT1G56220.5 72.093 86 22 2 1 84 26 111 4.79e-36 121
Msa1305070 AT1G54070.1 47.761 67 21 2 1 67 1 53 1.90e-11 57.4

Find 97 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACATGCATATTGAATAATTT+TGG 0.110350 8_3:+68381774 None:intergenic
TCTATATTCTCGATCCATTT+TGG 0.241117 8_3:-68381901 Msa1305070:three_prime_UTR
ACTGAAGATGTTACCCCTTT+TGG 0.271295 8_3:-68382279 Msa1305070:three_prime_UTR
GGGGTAACATCTTCAGTTTC+CGG 0.301859 8_3:+68382285 None:intergenic
ACGAGAGATTGCCAAGAAAA+AGG 0.317085 8_3:+68381943 None:intergenic
AAGAAGGGCCTGATCTGTTT+TGG 0.319411 8_3:+68382352 None:intergenic
AATTCATCCTCATACCAAAA+TGG 0.338716 8_3:+68381887 None:intergenic
AGTGAACAATGGAACAATAA+TGG 0.359458 8_3:-68381719 Msa1305070:three_prime_UTR
GTTAGGCATGGCGCTCTGTT+TGG 0.361146 8_3:-68382166 Msa1305070:three_prime_UTR
TGTGTATATAGATCCTTTAC+TGG 0.368772 8_3:-68382012 Msa1305070:three_prime_UTR
AGTGTAATAAGTTGTAAGTA+AGG 0.373833 8_3:-68382076 Msa1305070:three_prime_UTR
GTGAATAATAACGACCTTAC+CGG 0.378031 8_3:+68383394 None:intergenic
CGATCCCAATGACCAGATTC+CGG 0.418252 8_3:+68384180 None:intergenic
TACCCTTGCCAGAGCTAGAT+CGG 0.420036 8_3:+68384023 None:intergenic
CAGGAACTAGAGATTCCTTT+CGG 0.426315 8_3:-68382416 Msa1305070:CDS
CCGAGACCGTTCTCCGGCCG+AGG 0.426718 8_3:+68384076 None:intergenic
CGACGGTGTCGTCCCACAAC+TGG 0.429141 8_3:+68384104 None:intergenic
ACCGTTCTCCGGCCGAGGGC+CGG 0.433178 8_3:+68384081 None:intergenic
CTCCGGCCGAGGGCCGGCGA+CGG 0.445786 8_3:+68384087 None:intergenic
CACCGTCGCCGGCCCTCGGC+CGG 0.447404 8_3:-68384089 Msa1305070:CDS
GTATCTCTTACACCGGAATC+TGG 0.448206 8_3:-68384192 Msa1305070:five_prime_UTR
AGAACGGTCTCGGCAAGCTT+CGG 0.450915 8_3:-68384066 Msa1305070:CDS
TGGGCCTTCTCGACCAGTTG+TGG 0.456063 8_3:-68384117 Msa1305070:CDS
TTTGTGCTGCGGTTCACGTT+AGG 0.456617 8_3:-68382183 Msa1305070:three_prime_UTR
TACACCGGAATCTGGTCATT+GGG 0.458037 8_3:-68384184 Msa1305070:five_prime_UTR
TCGCCTCTGGCTTCTCCCGC+CGG 0.458567 8_3:-68383443 Msa1305070:CDS
AAATACGCCTGATCCATTCA+TGG 0.461198 8_3:-68384137 Msa1305070:exon
TATTACAGCAAAGGCCAAAA+GGG 0.461978 8_3:+68382265 None:intergenic
TTACACCGGAATCTGGTCAT+TGG 0.463228 8_3:-68384185 Msa1305070:five_prime_UTR
TACACCGCTATCCTACCCTT+TGG 0.465088 8_3:+68382049 None:intergenic
ACGACACCGTCGCCGGCCCT+CGG 0.467035 8_3:-68384093 Msa1305070:CDS
CCTCGGCCGGAGAACGGTCT+CGG 0.467575 8_3:-68384076 Msa1305070:CDS
TCAATATGCATGTCGGAAGA+TGG 0.467631 8_3:-68381764 Msa1305070:three_prime_UTR
TTATTACAGCAAAGGCCAAA+AGG 0.472228 8_3:+68382264 None:intergenic
CGCATTCAATCAGTGAACAA+TGG 0.473374 8_3:-68381730 Msa1305070:three_prime_UTR
CTTTCGGTTTCGACGAAGGT+CGG 0.476530 8_3:-68382400 Msa1305070:CDS
GCCGGCCCTCGGCCGGAGAA+CGG 0.477433 8_3:-68384082 Msa1305070:CDS
AGAAGGCCCATGAATGGATC+AGG 0.480654 8_3:+68384130 None:intergenic
ATAACGACCTTACCGGAAAA+CGG 0.482174 8_3:+68383401 None:intergenic
GTACGAAAGTGGATCGCCTC+TGG 0.484048 8_3:-68383456 Msa1305070:CDS
CGCCCGATCTAGCTCTGGCA+AGG 0.484849 8_3:-68384026 Msa1305070:CDS
AAGAAAGGCCAAAACAGATC+AGG 0.489997 8_3:-68382360 Msa1305070:CDS
ATCATGATCGTGAAACCGCC+AGG 0.492141 8_3:-68383479 Msa1305070:CDS
TTCGCCGCCCGATCTAGCTC+TGG 0.500632 8_3:-68384031 Msa1305070:CDS
AACAATAATGGACATGAAGA+AGG 0.503571 8_3:-68381707 Msa1305070:three_prime_UTR
GGGCCTTCTCGACCAGTTGT+GGG 0.507384 8_3:-68384116 Msa1305070:CDS
AGAGCTAGATCGGGCGGCGA+AGG 0.508256 8_3:+68384033 None:intergenic
TGTAAGTAAGGAAGTCCAAA+GGG 0.513200 8_3:-68382064 Msa1305070:three_prime_UTR
CGCAGTAGTATCTCTTACAC+CGG 0.520795 8_3:-68384199 Msa1305070:five_prime_UTR
TGGTCGAGAAGGCCCATGAA+TGG 0.522793 8_3:+68384124 None:intergenic
GTTTCGACGAAGGTCGGCAT+CGG 0.527766 8_3:-68382394 Msa1305070:CDS
CACACATCGATAGGAGAAGA+AGG 0.528061 8_3:+68382336 None:intergenic
ATAATGGACATGAAGAAGGT+TGG 0.529729 8_3:-68381703 Msa1305070:three_prime_UTR
ATTCCTTTCGGTTTCGACGA+AGG 0.530725 8_3:-68382404 Msa1305070:CDS
TCCCGCCGGTTCTACTACTC+CGG 0.530743 8_3:-68383429 Msa1305070:CDS
GTTTATTTGTGTGCATGTAT+CGG 0.533623 8_3:-68382231 Msa1305070:three_prime_UTR
CAATATGCATGTCGGAAGAT+GGG 0.533830 8_3:-68381763 Msa1305070:three_prime_UTR
ACCCTTGCCAGAGCTAGATC+GGG 0.537273 8_3:+68384024 None:intergenic
AGCTTGCCGAGACCGTTCTC+CGG 0.541689 8_3:+68384070 None:intergenic
GCTGCGGTTCACGTTAGGCA+TGG 0.543848 8_3:-68382178 Msa1305070:three_prime_UTR
GCGGGAAGCGTGAGATCGTA+CGG 0.546978 8_3:-68383542 Msa1305070:CDS
ATCGTACGGTGAGGAATCGT+CGG 0.548167 8_3:-68383528 Msa1305070:CDS
AGACACCGGAGTAGTAGAAC+CGG 0.549756 8_3:+68383424 None:intergenic
AAACCGCCAGGGTACGAAAG+TGG 0.556609 8_3:-68383467 Msa1305070:CDS
GAACCGGCGGGAGAAGCCAG+AGG 0.561661 8_3:+68383440 None:intergenic
CGAGACCGTTCTCCGGCCGA+GGG 0.565198 8_3:+68384077 None:intergenic
AATACGCCTGATCCATTCAT+GGG 0.567709 8_3:-68384136 Msa1305070:exon
GGCGATCCACTTTCGTACCC+TGG 0.568040 8_3:+68383461 None:intergenic
CGACCTTCGTCGAAACCGAA+AGG 0.571902 8_3:+68382401 None:intergenic
ATGGATCGAGAATATAGAGC+AGG 0.575307 8_3:+68381906 None:intergenic
TCGGATGCATACGAGAAGAA+AGG 0.583908 8_3:-68382375 Msa1305070:CDS
AAATTATTCAATATGCATGT+CGG 0.584174 8_3:-68381771 Msa1305070:three_prime_UTR
GCCCGATCTAGCTCTGGCAA+GGG 0.585634 8_3:-68384025 Msa1305070:intron
GATTGATCAGAAATGGAGGC+GGG 0.586985 8_3:-68383560 Msa1305070:intron
CGTCCCACAACTGGTCGAGA+AGG 0.590163 8_3:+68384113 None:intergenic
CATGTATCGGTGTAATGCAT+TGG 0.591207 8_3:-68382218 Msa1305070:three_prime_UTR
ACACTTGATAATGTGACAGC+CGG 0.591912 8_3:-68381802 Msa1305070:three_prime_UTR
TTGTGGGACGACACCGTCGC+CGG 0.591995 8_3:-68384100 Msa1305070:CDS
TTGTAAGTAAGGAAGTCCAA+AGG 0.595220 8_3:-68382065 Msa1305070:three_prime_UTR
CTCATTTCTCACACATCGAT+AGG 0.601241 8_3:+68382327 None:intergenic
TGATTGATCAGAAATGGAGG+CGG 0.603270 8_3:-68383561 Msa1305070:intron
GTGTATATAGATCCTTTACT+GGG 0.611023 8_3:-68382011 Msa1305070:three_prime_UTR
TCTTCATGGGATGGCTACAG+AGG 0.611027 8_3:-68381830 Msa1305070:three_prime_UTR
AGTAAGGAAGTCCAAAGGGT+AGG 0.612018 8_3:-68382060 Msa1305070:three_prime_UTR
AATTGATTGATCAGAAATGG+AGG 0.625094 8_3:-68383564 Msa1305070:intron
TTACCGGAAAACGGAGACAC+CGG 0.636278 8_3:+68383410 None:intergenic
ACACATCGATAGGAGAAGAA+GGG 0.647952 8_3:+68382337 None:intergenic
CACCGGAGTAGTAGAACCGG+CGG 0.650193 8_3:+68383427 None:intergenic
ATTACAGCAAAGGCCAAAAG+GGG 0.651252 8_3:+68382266 None:intergenic
TCATGATCGTGAAACCGCCA+GGG 0.653940 8_3:-68383478 Msa1305070:CDS
CTTGCCAGAGCTAGATCGGG+CGG 0.658767 8_3:+68384027 None:intergenic
AAGTCCAAAGGGTAGGATAG+CGG 0.662147 8_3:-68382053 Msa1305070:three_prime_UTR
ACCGGAGTAGTAGAACCGGC+GGG 0.676139 8_3:+68383428 None:intergenic
CCGGAGAAGATAAAATGCGG+TGG 0.684216 8_3:+68382304 None:intergenic
GATCCACTTTCGTACCCTGG+CGG 0.709901 8_3:+68383464 None:intergenic
AAGCGTGAGATCGTACGGTG+AGG 0.726983 8_3:-68383537 Msa1305070:CDS
TTTCCGGAGAAGATAAAATG+CGG 0.732385 8_3:+68382301 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATAAGTTTTTTTTTGA+AGG - chr8_3:68382661-68382680 Msa1305070:intron 10.0%
!! ATTATATTGTCATATATTTA+AGG - chr8_3:68383206-68383225 Msa1305070:intron 10.0%
!! ATCATTTATAATAACAATAA+TGG - chr8_3:68383286-68383305 Msa1305070:intron 10.0%
!! AAAAAAAAAAACTTATTACA+CGG + chr8_3:68383762-68383781 None:intergenic 10.0%
!!! AGTTTTTTTTTTTTTTTTGA+AGG - chr8_3:68383769-68383788 Msa1305070:intron 10.0%
!! AAATTATATACGAGAAATTA+AGG + chr8_3:68381889-68381908 None:intergenic 15.0%
!! AAAATTTAAAAGCAAAAGTT+AGG + chr8_3:68382632-68382651 None:intergenic 15.0%
!! TTGAAGGAATAATTAATATT+AGG - chr8_3:68382677-68382696 Msa1305070:intron 15.0%
!! AACCAACTTATTAATATTAA+GGG + chr8_3:68382974-68382993 None:intergenic 15.0%
!! TAACCAACTTATTAATATTA+AGG + chr8_3:68382975-68382994 None:intergenic 15.0%
!!! TTTATAATAACAATAATGGA+AGG - chr8_3:68383290-68383309 Msa1305070:intron 15.0%
!! TGAAGGTTAATCAATATTAA+CGG + chr8_3:68382010-68382029 None:intergenic 20.0%
!!! AAGCTTAAAGTTTGAAATTA+TGG - chr8_3:68382085-68382104 Msa1305070:three_prime_UTR 20.0%
!! ATTAGGCTTAAATATGTTAT+TGG - chr8_3:68382694-68382713 Msa1305070:intron 20.0%
!! GAAACATTTATTGAAATGTA+AGG - chr8_3:68382900-68382919 Msa1305070:intron 20.0%
!!! TTAAGGGTTAAATATGTTTT+TGG + chr8_3:68382958-68382977 None:intergenic 20.0%
!! AACCCTTAATATTAATAAGT+TGG - chr8_3:68382969-68382988 Msa1305070:intron 20.0%
!! GAAAAAAATTATTACAGCAA+AGG + chr8_3:68383615-68383634 None:intergenic 20.0%
!!! AGATTCTTTTAAGTCTTATT+AGG - chr8_3:68383732-68383751 Msa1305070:intron 20.0%
!!! CATGCATATTGAATAATTTT+GGG + chr8_3:68384096-68384115 None:intergenic 20.0%
!!! ACATGCATATTGAATAATTT+TGG + chr8_3:68384097-68384116 None:intergenic 20.0%
!! AAATTATTCAATATGCATGT+CGG - chr8_3:68384097-68384116 Msa1305070:CDS 20.0%
! TTATATACGAGAAATTAAGG+TGG + chr8_3:68381886-68381905 None:intergenic 25.0%
! CAATTCGATATTCAATTTGA+AGG + chr8_3:68382027-68382046 None:intergenic 25.0%
!!! AGTTCTTGCATGTTTTATTT+TGG - chr8_3:68382154-68382173 Msa1305070:three_prime_UTR 25.0%
! GAAAATTGATTGATCAGAAA+TGG - chr8_3:68382301-68382320 Msa1305070:three_prime_UTR 25.0%
! TTTACTTCACTTTACTTTAC+TGG + chr8_3:68382560-68382579 None:intergenic 25.0%
! GTAAATGATCTCCTAAATTT+CGG - chr8_3:68382575-68382594 Msa1305070:intron 25.0%
! TAAATGATCTCCTAAATTTC+GGG - chr8_3:68382576-68382595 Msa1305070:intron 25.0%
!! TGCTATATTTTGTCTTTTTG+TGG - chr8_3:68382601-68382620 Msa1305070:intron 25.0%
!! TATTTTGTCTTTTTGTGGAA+TGG - chr8_3:68382606-68382625 Msa1305070:intron 25.0%
! GATAACAGAGTAAAAATGTA+AGG + chr8_3:68382781-68382800 None:intergenic 25.0%
!! ATACTACGGTTTCTAAATTT+TGG + chr8_3:68382831-68382850 None:intergenic 25.0%
! CAAAACTTCAACAAATACTA+CGG + chr8_3:68382845-68382864 None:intergenic 25.0%
! TCAAAGTCGTGTAAAATATA+TGG - chr8_3:68382931-68382950 Msa1305070:intron 25.0%
!!! GTGCTATTTTATTAATTGTG+TGG - chr8_3:68383146-68383165 Msa1305070:intron 25.0%
!!! CTAGTTGTCTATTTTAACTT+GGG + chr8_3:68383242-68383261 None:intergenic 25.0%
! AGTGTAATAAGTTGTAAGTA+AGG - chr8_3:68383792-68383811 Msa1305070:intron 25.0%
!! CTGAATAATAACCTTTTTCT+TGG - chr8_3:68383914-68383933 Msa1305070:intron 25.0%
!!! CATATTGAATAATTTTGGGT+GGG + chr8_3:68384092-68384111 None:intergenic 25.0%
!! AATTTTAGCGATAACCGTTT+TGG + chr8_3:68381941-68381960 None:intergenic 30.0%
TGAATATCGAATTGTGTTCT+AGG - chr8_3:68382033-68382052 Msa1305070:three_prime_UTR 30.0%
AATTGATTGATCAGAAATGG+AGG - chr8_3:68382304-68382323 Msa1305070:three_prime_UTR 30.0%
! AGTTTTACTGTCAAGAAACA+CGG + chr8_3:68382515-68382534 None:intergenic 30.0%
!!! CACTTTACTTTACTGGTTTT+TGG + chr8_3:68382553-68382572 None:intergenic 30.0%
!! GTTTCTAAATTTTGGTGACT+AGG + chr8_3:68382823-68382842 None:intergenic 30.0%
TTGTTCAACTTGAACAAAAC+CGG - chr8_3:68382862-68382881 Msa1305070:intron 30.0%
!! TTTCTAAAATCCCTACATTC+CGG + chr8_3:68382884-68382903 None:intergenic 30.0%
!!! CCTAGTTGTCTATTTTAACT+TGG + chr8_3:68383243-68383262 None:intergenic 30.0%
CCAAGTTAAAATAGACAACT+AGG - chr8_3:68383240-68383259 Msa1305070:intron 30.0%
GTTTATTTGTGTGCATGTAT+CGG - chr8_3:68383637-68383656 Msa1305070:intron 30.0%
TGTGTATATAGATCCTTTAC+TGG - chr8_3:68383856-68383875 Msa1305070:intron 30.0%
GTGTATATAGATCCTTTACT+GGG - chr8_3:68383857-68383876 Msa1305070:intron 30.0%
! TCTATATTCTCGATCCATTT+TGG - chr8_3:68383967-68383986 Msa1305070:intron 30.0%
AATTCATCCTCATACCAAAA+TGG + chr8_3:68383984-68384003 None:intergenic 30.0%
! TGGAACATTTTTGTCTTCAT+GGG - chr8_3:68384025-68384044 Msa1305070:intron 30.0%
! GCATATTGAATAATTTTGGG+TGG + chr8_3:68384093-68384112 None:intergenic 30.0%
AGTGAACAATGGAACAATAA+TGG - chr8_3:68384149-68384168 Msa1305070:five_prime_UTR 30.0%
!! AACAATAATGGACATGAAGA+AGG - chr8_3:68384161-68384180 Msa1305070:five_prime_UTR 30.0%
GTGAATAATAACGACCTTAC+CGG + chr8_3:68382477-68382496 None:intergenic 35.0%
! GTTTTACTGTCAAGAAACAC+GGG + chr8_3:68382514-68382533 None:intergenic 35.0%
!!! ACTTTACTGGTTTTTGGAAC+AGG + chr8_3:68382547-68382566 None:intergenic 35.0%
ATATAGCATGCCCGAAATTT+AGG + chr8_3:68382589-68382608 None:intergenic 35.0%
TTGAACAAAACCGGAATGTA+GGG - chr8_3:68382871-68382890 Msa1305070:intron 35.0%
GACAACTAGGTTAAGTTACT+TGG - chr8_3:68383253-68383272 Msa1305070:intron 35.0%
TTTCCGGAGAAGATAAAATG+CGG + chr8_3:68383570-68383589 None:intergenic 35.0%
TATTACAGCAAAGGCCAAAA+GGG + chr8_3:68383606-68383625 None:intergenic 35.0%
TTATTACAGCAAAGGCCAAA+AGG + chr8_3:68383607-68383626 None:intergenic 35.0%
!! TGAATTGAAGTTTTGTGCTG+CGG - chr8_3:68383674-68383693 Msa1305070:intron 35.0%
TTGTAAGTAAGGAAGTCCAA+AGG - chr8_3:68383803-68383822 Msa1305070:intron 35.0%
TGTAAGTAAGGAAGTCCAAA+GGG - chr8_3:68383804-68383823 Msa1305070:intron 35.0%
!!! AAGTCCAAGAAGTGTTTTTG+TGG - chr8_3:68384005-68384024 Msa1305070:intron 35.0%
TGTTCCACAAAAACACTTCT+TGG + chr8_3:68384012-68384031 None:intergenic 35.0%
! GTGGAACATTTTTGTCTTCA+TGG - chr8_3:68384024-68384043 Msa1305070:intron 35.0%
! ACATTTTTGTCTTCATGGGA+TGG - chr8_3:68384029-68384048 Msa1305070:CDS 35.0%
!! ATAATGGACATGAAGAAGGT+TGG - chr8_3:68384165-68384184 Msa1305070:five_prime_UTR 35.0%
AAATACGCCTGATCCATTCA+TGG - chr8_3:68381731-68381750 Msa1305070:three_prime_UTR 40.0%
AATACGCCTGATCCATTCAT+GGG - chr8_3:68381732-68381751 Msa1305070:three_prime_UTR 40.0%
! TCTTTGAGCTTTTCACATGC+AGG + chr8_3:68382191-68382210 None:intergenic 40.0%
TGATTGATCAGAAATGGAGG+CGG - chr8_3:68382307-68382326 Msa1305070:three_prime_UTR 40.0%
ATAACGACCTTACCGGAAAA+CGG + chr8_3:68382470-68382489 None:intergenic 40.0%
TCAAAGTCGCGCAAAATGTA+GGG + chr8_3:68382721-68382740 None:intergenic 40.0%
TTCAAAGTCGCGCAAAATGT+AGG + chr8_3:68382722-68382741 None:intergenic 40.0%
GGTGACTAGGAAGAAATTGA+CGG + chr8_3:68382810-68382829 None:intergenic 40.0%
CTTGAACAAAACCGGAATGT+AGG - chr8_3:68382870-68382889 Msa1305070:intron 40.0%
!! GTAAGTTGATACGAGACTTG+TGG + chr8_3:68383002-68383021 None:intergenic 40.0%
GTCTCGTATCAACTTACAAG+CGG - chr8_3:68383005-68383024 Msa1305070:intron 40.0%
TTAAAACCCACACTCTTCCA+AGG + chr8_3:68383108-68383127 None:intergenic 40.0%
!! GCGATGCGTGTTTATTTTTC+AGG - chr8_3:68383433-68383452 Msa1305070:CDS 40.0%
CAGGAACTAGAGATTCCTTT+CGG - chr8_3:68383452-68383471 Msa1305070:CDS 40.0%
AAGAAAGGCCAAAACAGATC+AGG - chr8_3:68383508-68383527 Msa1305070:CDS 40.0%
ACACATCGATAGGAGAAGAA+GGG + chr8_3:68383534-68383553 None:intergenic 40.0%
CTCATTTCTCACACATCGAT+AGG + chr8_3:68383544-68383563 None:intergenic 40.0%
! ACTGAAGATGTTACCCCTTT+TGG - chr8_3:68383589-68383608 Msa1305070:intron 40.0%
ATTACAGCAAAGGCCAAAAG+GGG + chr8_3:68383605-68383624 None:intergenic 40.0%
! CATGTATCGGTGTAATGCAT+TGG - chr8_3:68383650-68383669 Msa1305070:intron 40.0%
GAAAAACAACAGCCCAGTAA+AGG + chr8_3:68383872-68383891 None:intergenic 40.0%
ACGAGAGATTGCCAAGAAAA+AGG + chr8_3:68383928-68383947 None:intergenic 40.0%
ATGGATCGAGAATATAGAGC+AGG + chr8_3:68383965-68383984 None:intergenic 40.0%
!! TCTCGATCCATTTTGGTATG+AGG - chr8_3:68383974-68383993 Msa1305070:intron 40.0%
!! ACACTTGATAATGTGACAGC+CGG - chr8_3:68384066-68384085 Msa1305070:CDS 40.0%
!! TTGAATAATTTTGGGTGGGC+CGG + chr8_3:68384088-68384107 None:intergenic 40.0%
TCAATATGCATGTCGGAAGA+TGG - chr8_3:68384104-68384123 Msa1305070:CDS 40.0%
CAATATGCATGTCGGAAGAT+GGG - chr8_3:68384105-68384124 Msa1305070:CDS 40.0%
CGCATTCAATCAGTGAACAA+TGG - chr8_3:68384138-68384157 Msa1305070:exon 40.0%
CGCAGTAGTATCTCTTACAC+CGG - chr8_3:68381669-68381688 Msa1305070:three_prime_UTR 45.0%
GTATCTCTTACACCGGAATC+TGG - chr8_3:68381676-68381695 Msa1305070:three_prime_UTR 45.0%
TTACACCGGAATCTGGTCAT+TGG - chr8_3:68381683-68381702 Msa1305070:three_prime_UTR 45.0%
TACACCGGAATCTGGTCATT+GGG - chr8_3:68381684-68381703 Msa1305070:three_prime_UTR 45.0%
!!! CAGGCGTATTTTAGCAGATG+CGG + chr8_3:68381722-68381741 None:intergenic 45.0%
ATTAAGGTGGCAGAGTACAG+CGG + chr8_3:68381873-68381892 None:intergenic 45.0%
ACGAATCACCAGAGCCAAAA+CGG - chr8_3:68381924-68381943 Msa1305070:three_prime_UTR 45.0%
GATTGATCAGAAATGGAGGC+GGG - chr8_3:68382308-68382327 Msa1305070:three_prime_UTR 45.0%
CTAGGAAGAAATTGACGGCA+GGG + chr8_3:68382805-68382824 None:intergenic 45.0%
ACTAGGAAGAAATTGACGGC+AGG + chr8_3:68382806-68382825 None:intergenic 45.0%
TAAATCAGTCGCTCTGACTG+AGG - chr8_3:68383070-68383089 Msa1305070:intron 45.0%
!!! GGAAGAGTGTGGGTTTTAAG+CGG - chr8_3:68383109-68383128 Msa1305070:intron 45.0%
ATTCCTTTCGGTTTCGACGA+AGG - chr8_3:68383464-68383483 Msa1305070:CDS 45.0%
TCGGATGCATACGAGAAGAA+AGG - chr8_3:68383493-68383512 Msa1305070:CDS 45.0%
! AAGAAGGGCCTGATCTGTTT+TGG + chr8_3:68383519-68383538 None:intergenic 45.0%
CACACATCGATAGGAGAAGA+AGG + chr8_3:68383535-68383554 None:intergenic 45.0%
! CCACCGCATTTTATCTTCTC+CGG - chr8_3:68383564-68383583 Msa1305070:intron 45.0%
GGGGTAACATCTTCAGTTTC+CGG + chr8_3:68383586-68383605 None:intergenic 45.0%
AGTAAGGAAGTCCAAAGGGT+AGG - chr8_3:68383808-68383827 Msa1305070:intron 45.0%
AAGTCCAAAGGGTAGGATAG+CGG - chr8_3:68383815-68383834 Msa1305070:intron 45.0%
CGATCCCAATGACCAGATTC+CGG + chr8_3:68381691-68381710 None:intergenic 50.0%
AGAAGGCCCATGAATGGATC+AGG + chr8_3:68381741-68381760 None:intergenic 50.0%
TACCCTTGCCAGAGCTAGAT+CGG + chr8_3:68381848-68381867 None:intergenic 50.0%
!!! AGCGATAACCGTTTTGGCTC+TGG + chr8_3:68381935-68381954 None:intergenic 50.0%
ATCGTACGGTGAGGAATCGT+CGG - chr8_3:68382340-68382359 Msa1305070:CDS 50.0%
ATCATGATCGTGAAACCGCC+AGG - chr8_3:68382389-68382408 Msa1305070:CDS 50.0%
TCATGATCGTGAAACCGCCA+GGG - chr8_3:68382390-68382409 Msa1305070:CDS 50.0%
AGACACCGGAGTAGTAGAAC+CGG + chr8_3:68382447-68382466 None:intergenic 50.0%
TTACCGGAAAACGGAGACAC+CGG + chr8_3:68382461-68382480 None:intergenic 50.0%
TGAGGTTTGAACCTCGACCT+TGG - chr8_3:68383088-68383107 Msa1305070:intron 50.0%
CTTTCGGTTTCGACGAAGGT+CGG - chr8_3:68383468-68383487 Msa1305070:CDS 50.0%
CCGGAGAAGATAAAATGCGG+TGG + chr8_3:68383567-68383586 None:intergenic 50.0%
TTTGTGCTGCGGTTCACGTT+AGG - chr8_3:68383685-68383704 Msa1305070:intron 50.0%
TACACCGCTATCCTACCCTT+TGG + chr8_3:68383822-68383841 None:intergenic 50.0%
TCTTCATGGGATGGCTACAG+AGG - chr8_3:68384038-68384057 Msa1305070:CDS 50.0%
TGGTCGAGAAGGCCCATGAA+TGG + chr8_3:68381747-68381766 None:intergenic 55.0%
AGAACGGTCTCGGCAAGCTT+CGG - chr8_3:68381802-68381821 Msa1305070:three_prime_UTR 55.0%
ACCCTTGCCAGAGCTAGATC+GGG + chr8_3:68381847-68381866 None:intergenic 55.0%
AAGCGTGAGATCGTACGGTG+AGG - chr8_3:68382331-68382350 Msa1305070:CDS 55.0%
AAACCGCCAGGGTACGAAAG+TGG - chr8_3:68382401-68382420 Msa1305070:CDS 55.0%
GATCCACTTTCGTACCCTGG+CGG + chr8_3:68382407-68382426 None:intergenic 55.0%
GTACGAAAGTGGATCGCCTC+TGG - chr8_3:68382412-68382431 Msa1305070:CDS 55.0%
! ACTCCGGTGTCTCCGTTTTC+CGG - chr8_3:68382455-68382474 Msa1305070:intron 55.0%
! GGTGTCTCCGTTTTCCGGTA+AGG - chr8_3:68382460-68382479 Msa1305070:intron 55.0%
ACCTCGACCTTGGAAGAGTG+TGG - chr8_3:68383098-68383117 Msa1305070:intron 55.0%
! CCTCGACCTTGGAAGAGTGT+GGG - chr8_3:68383099-68383118 Msa1305070:intron 55.0%
CGACCTTCGTCGAAACCGAA+AGG + chr8_3:68383470-68383489 None:intergenic 55.0%
GTTTCGACGAAGGTCGGCAT+CGG - chr8_3:68383474-68383493 Msa1305070:CDS 55.0%
GTTAGGCATGGCGCTCTGTT+TGG - chr8_3:68383702-68383721 Msa1305070:intron 55.0%
TGGGCCTTCTCGACCAGTTG+TGG - chr8_3:68381751-68381770 Msa1305070:three_prime_UTR 60.0%
GGGCCTTCTCGACCAGTTGT+GGG - chr8_3:68381752-68381771 Msa1305070:three_prime_UTR 60.0%
CGTCCCACAACTGGTCGAGA+AGG + chr8_3:68381758-68381777 None:intergenic 60.0%
AGCTTGCCGAGACCGTTCTC+CGG + chr8_3:68381801-68381820 None:intergenic 60.0%
CTTGCCAGAGCTAGATCGGG+CGG + chr8_3:68381844-68381863 None:intergenic 60.0%
!! GCCCGATCTAGCTCTGGCAA+GGG - chr8_3:68381843-68381862 Msa1305070:three_prime_UTR 60.0%
! GCGGGAAGCGTGAGATCGTA+CGG - chr8_3:68382326-68382345 Msa1305070:exon 60.0%
GGCGATCCACTTTCGTACCC+TGG + chr8_3:68382410-68382429 None:intergenic 60.0%
!! TCCCGCCGGTTCTACTACTC+CGG - chr8_3:68382439-68382458 Msa1305070:intron 60.0%
ACCGGAGTAGTAGAACCGGC+GGG + chr8_3:68382443-68382462 None:intergenic 60.0%
CACCGGAGTAGTAGAACCGG+CGG + chr8_3:68382444-68382463 None:intergenic 60.0%
CCCACACTCTTCCAAGGTCG+AGG + chr8_3:68383102-68383121 None:intergenic 60.0%
GCTGCGGTTCACGTTAGGCA+TGG - chr8_3:68383690-68383709 Msa1305070:intron 60.0%
!! CGACGGTGTCGTCCCACAAC+TGG + chr8_3:68381767-68381786 None:intergenic 65.0%
TTGTGGGACGACACCGTCGC+CGG - chr8_3:68381768-68381787 Msa1305070:three_prime_UTR 65.0%
AGAGCTAGATCGGGCGGCGA+AGG + chr8_3:68381838-68381857 None:intergenic 65.0%
! TTCGCCGCCCGATCTAGCTC+TGG - chr8_3:68381837-68381856 Msa1305070:three_prime_UTR 65.0%
!! CGCCCGATCTAGCTCTGGCA+AGG - chr8_3:68381842-68381861 Msa1305070:three_prime_UTR 65.0%
CGAGACCGTTCTCCGGCCGA+GGG + chr8_3:68381794-68381813 None:intergenic 70.0%
CCTCGGCCGGAGAACGGTCT+CGG - chr8_3:68381792-68381811 Msa1305070:three_prime_UTR 70.0%
! TCGCCTCTGGCTTCTCCCGC+CGG - chr8_3:68382425-68382444 Msa1305070:intron 70.0%
GAACCGGCGGGAGAAGCCAG+AGG + chr8_3:68382431-68382450 None:intergenic 70.0%
ACGACACCGTCGCCGGCCCT+CGG - chr8_3:68381775-68381794 Msa1305070:three_prime_UTR 75.0%
ACCGTTCTCCGGCCGAGGGC+CGG + chr8_3:68381790-68381809 None:intergenic 75.0%
CCGAGACCGTTCTCCGGCCG+AGG + chr8_3:68381795-68381814 None:intergenic 75.0%
! GCCGGCCCTCGGCCGGAGAA+CGG - chr8_3:68381786-68381805 Msa1305070:three_prime_UTR 80.0%
! CACCGTCGCCGGCCCTCGGC+CGG - chr8_3:68381779-68381798 Msa1305070:three_prime_UTR 85.0%
! CTCCGGCCGAGGGCCGGCGA+CGG + chr8_3:68381784-68381803 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 68381663 68384227 68381663 ID=Msa1305070;Name=Msa1305070
chr8_3 mRNA 68381663 68384227 68381663 ID=Msa1305070-mRNA-1;Parent=Msa1305070;Name=Msa1305070-mRNA-1;_AED=0.03;_eAED=0.03;_QI=87|0.5|0.66|1|0.5|0.66|3|665|128
chr8_3 exon 68381663 68382435 68381663 ID=Msa1305070-mRNA-1:exon:21155;Parent=Msa1305070-mRNA-1
chr8_3 exon 68383409 68383572 68383409 ID=Msa1305070-mRNA-1:exon:21154;Parent=Msa1305070-mRNA-1
chr8_3 exon 68384026 68384227 68384026 ID=Msa1305070-mRNA-1:exon:21153;Parent=Msa1305070-mRNA-1
chr8_3 five_prime_UTR 68384141 68384227 68384141 ID=Msa1305070-mRNA-1:five_prime_utr;Parent=Msa1305070-mRNA-1
chr8_3 CDS 68384026 68384140 68384026 ID=Msa1305070-mRNA-1:cds;Parent=Msa1305070-mRNA-1
chr8_3 CDS 68383409 68383572 68383409 ID=Msa1305070-mRNA-1:cds;Parent=Msa1305070-mRNA-1
chr8_3 CDS 68382328 68382435 68382328 ID=Msa1305070-mRNA-1:cds;Parent=Msa1305070-mRNA-1
chr8_3 three_prime_UTR 68381663 68382327 68381663 ID=Msa1305070-mRNA-1:three_prime_utr;Parent=Msa1305070-mRNA-1
Gene Sequence

>Msa1305070

ATGGGCCTTCTCGACCAGTTGTGGGACGACACCGTCGCCGGCCCTCGGCCGGAGAACGGTCTCGGCAAGCTTCGGAAGCAAAACACCTTCGCCGCCCGATCTAGCTCTGGCAAGGAAATGGAGGCGGGAAGCGTGAGATCGTACGGTGAGGAATCGTCGGAGCAAACGACGAGAGTAACACGTAGCATCATGATCGTGAAACCGCCAGGGTACGAAAGTGGATCGCCTCTGGCTTCTCCCGCCGGTTCTACTACTCCGGTGTCTCCGTTTTCCGGTAAGGAACTAGAGATTCCTTTCGGTTTCGACGAAGGTCGGCATCGGATGCATACGAGAAGAAAGGCCAAAACAGATCAGGCCCTTCTTCTCCTATCGATGTGTGAGAAATGA
Protein sequence

>Msa1305070

MGLLDQLWDDTVAGPRPENGLGKLRKQNTFAARSSSGKEMEAGSVRSYGEESSEQTTRVTRSIMIVKPPGYESGSPLASPAGSTTPVSPFSGKELEIPFGFDEGRHRMHTRRKAKTDQALLLLSMCEK*