AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0625590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625590 MtrunA17_Chr4g0002531 98.658 149 2 0 1 149 92 240 1.71e-107 306
Msa0625590 MtrunA17_Chr3g0137121 50.000 126 53 3 1 123 82 200 1.44e-35 122
Msa0625590 MtrunA17_Chr1g0149791 66.667 78 26 0 1 78 82 159 2.16e-35 122
Msa0625590 MtrunA17_Chr5g0399541 56.250 80 35 0 1 80 88 167 1.47e-30 109
Msa0625590 MtrunA17_Chr1g0162711 49.020 102 48 2 1 101 106 204 4.59e-30 109
Msa0625590 MtrunA17_Chr4g0055821 52.500 80 38 0 1 80 87 166 2.21e-28 103
Msa0625590 MtrunA17_Chr1g0154681 47.573 103 50 2 1 102 107 206 5.41e-27 101
Msa0625590 MtrunA17_Chr2g0306871 38.525 122 62 3 2 110 92 213 2.74e-24 93.6
Msa0625590 MtrunA17_Chr4g0063261 42.391 92 51 1 2 91 95 186 1.51e-23 91.7
Msa0625590 MtrunA17_Chr1g0202701 45.161 62 34 0 1 62 80 141 1.70e-15 70.5
Msa0625590 MtrunA17_Chr6g0468771 45.161 62 34 0 1 62 81 142 2.18e-15 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625590 AT1G80690.1 63.107 103 34 3 1 102 80 179 4.10e-41 137
Msa0625590 AT1G80690.2 60.748 107 34 4 1 102 80 183 6.70e-39 131
Msa0625590 AT5G25170.2 68.750 80 25 0 1 80 39 118 6.00e-37 125
Msa0625590 AT5G25170.1 59.794 97 39 0 1 97 83 179 3.49e-36 124
Msa0625590 AT2G25190.1 64.557 79 28 0 1 79 82 160 3.44e-34 120
Msa0625590 AT4G31980.1 65.823 79 27 0 1 79 82 160 1.11e-32 122
Msa0625590 AT1G47740.1 49.000 100 47 1 1 100 134 229 4.94e-28 105
Msa0625590 AT1G47740.2 49.000 100 47 1 1 100 134 229 4.94e-28 105
Msa0625590 AT1G47740.3 49.000 100 47 1 1 100 134 229 4.94e-28 105
Msa0625590 AT4G17486.1 40.351 114 64 1 1 110 91 204 6.69e-26 98.2
Msa0625590 AT5G47310.1 46.250 80 43 0 1 80 93 172 4.96e-25 96.3
Msa0625590 AT4G17486.2 50.820 61 30 0 1 61 91 151 1.82e-18 78.2
Msa0625590 AT4G25660.1 33.077 130 83 2 1 129 81 207 8.10e-15 69.7
Msa0625590 AT4G25680.1 34.437 151 85 6 1 143 81 225 5.04e-14 67.4

Find 40 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCAATATGTTGCCTTTCT+TGG 0.188301 4_3:+4764179 None:intergenic
GATAGCAACTATTGTAAAAT+TGG 0.213801 4_3:+4764208 None:intergenic
CTTGAAACATTAACCTTGTT+TGG 0.254573 4_3:+4764393 None:intergenic
GATGCTTGTGTTAAGCTTAC+TGG 0.266107 4_3:-4767202 Msa0625590:CDS
CATGGTGTTGAATATGCATT+TGG 0.344242 4_3:-4767424 Msa0625590:five_prime_UTR
AATGTGAAGGGTTCAGATTT+AGG 0.352563 4_3:-4767354 Msa0625590:five_prime_UTR
TGGAAATTCTATACCTAGTT+GGG 0.371552 4_3:-4767182 Msa0625590:CDS
CTGGAAATTCTATACCTAGT+TGG 0.371967 4_3:-4767183 Msa0625590:CDS
GTTAATCGTCTTGCTAGAAT+TGG 0.379939 4_3:-4767160 Msa0625590:intron
ATTAGTACATGATTCCTTGC+TGG 0.385277 4_3:+4764079 None:intergenic
TCACCGGCTTCTTCTAGCTC+TGG 0.400392 4_3:-4764348 Msa0625590:CDS
CTGAACCCTTCACATTTCCT+TGG 0.401139 4_3:+4767361 None:intergenic
TTTAGGAAGACAATAATGAT+TGG 0.412372 4_3:-4767337 Msa0625590:exon
ATGGTGTTGAATATGCATTT+GGG 0.452149 4_3:-4767423 Msa0625590:five_prime_UTR
TGGTTCTCCTTCAAATATAC+CGG 0.461685 4_3:+4767381 None:intergenic
TTCTAGCTCTGGATTAAGAC+GGG 0.465458 4_3:-4764337 Msa0625590:CDS
GCCGGTGAAGATGCTCTTGA+AGG 0.484906 4_3:+4764363 None:intergenic
TGCTATCAACAAACCAAGAA+AGG 0.486381 4_3:-4764192 Msa0625590:three_prime_UTR
GCACATGAGTATGCATCAAC+CGG 0.506681 4_3:-4767400 Msa0625590:five_prime_UTR
GATTAAGACGGGGAAGGAGC+AGG 0.510693 4_3:-4764326 Msa0625590:CDS
GCATCAACCGGTATATTTGA+AGG 0.517962 4_3:-4767388 Msa0625590:five_prime_UTR
ACCTTCAAGAGCATCTTCAC+CGG 0.528352 4_3:-4764364 Msa0625590:CDS
TTTGTTGAGTTCAATGTCAC+GGG 0.532965 4_3:+4764495 None:intergenic
CTTCTAGCTCTGGATTAAGA+CGG 0.562003 4_3:-4764338 Msa0625590:CDS
TGGTGAAGTGAGAAGTGTAA+TGG 0.565751 4_3:-4767296 Msa0625590:CDS
GCAATAATCAAAGGTGAAGG+TGG 0.569859 4_3:+4764291 None:intergenic
TAAACATCAAGATGAAGGGG+AGG 0.607838 4_3:+4764269 None:intergenic
GGAGAACCAAGGAAATGTGA+AGG 0.613559 4_3:-4767367 Msa0625590:five_prime_UTR
ATAATCAAAGGTGAAGGTGG+AGG 0.615233 4_3:+4764294 None:intergenic
AATCCAGAGCTAGAAGAAGC+CGG 0.616375 4_3:+4764345 None:intergenic
ATTAACAAGTGAACCAAACA+AGG 0.618419 4_3:-4764406 Msa0625590:CDS
GAACTTGCTGCTGAGTACAA+AGG 0.625237 4_3:-4767271 Msa0625590:CDS
GAAGGGGAGGCAATAATCAA+AGG 0.625643 4_3:+4764282 None:intergenic
GCAAGACGATTAACCCAACT+AGG 0.629110 4_3:+4767169 None:intergenic
TGGTGTTGAATATGCATTTG+GGG 0.634217 4_3:-4767422 Msa0625590:five_prime_UTR
GAGGCAATAATCAAAGGTGA+AGG 0.662473 4_3:+4764288 None:intergenic
GAGAACCAAGGAAATGTGAA+GGG 0.662717 4_3:-4767366 Msa0625590:five_prime_UTR
TCTAGCTCTGGATTAAGACG+GGG 0.680990 4_3:-4764336 Msa0625590:CDS
GCTCTGGATTAAGACGGGGA+AGG 0.690217 4_3:-4764332 Msa0625590:CDS
GTATATTTGAAGGAGAACCA+AGG 0.721199 4_3:-4767378 Msa0625590:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAATTAAAATTAAT+TGG + chr4_3:4766167-4766186 None:intergenic 0.0%
!! ATAAATTGAAATAATTAGTT+AGG - chr4_3:4764467-4764486 Msa0625590:CDS 10.0%
!!! TCATAGTTTTTTTTTTTTTA+AGG - chr4_3:4764735-4764754 Msa0625590:intron 10.0%
!!! TTTACATTTAATTTAAAGAA+AGG - chr4_3:4765602-4765621 Msa0625590:intron 10.0%
!! TTTATATGCATTTAAATATA+TGG - chr4_3:4765744-4765763 Msa0625590:intron 10.0%
!!! TATTAATTTTAAAATGCATA+AGG - chr4_3:4765639-4765658 Msa0625590:intron 10.0%
!! TTTATATGCATTTAAATATA+TGG - chr4_3:4765744-4765763 Msa0625590:intron 10.0%
!! TTTGTAACAAAAAAAAAAAT+AGG - chr4_3:4766812-4766831 Msa0625590:intron 10.0%
!! AAAAAATGTATAATAAAAGT+AGG - chr4_3:4766842-4766861 Msa0625590:intron 10.0%
!!! TTTTTTTTTTTTTCCTGTTT+TGG - chr4_3:4764410-4764429 Msa0625590:CDS 15.0%
!!! ATTTCAATTTATTTGTTTCT+TGG + chr4_3:4764459-4764478 None:intergenic 15.0%
!!! TCAAAAAATGTATTTTTTCT+TGG - chr4_3:4765112-4765131 Msa0625590:intron 15.0%
!!! CAAAAAATGTATTTTTTCTT+GGG - chr4_3:4765113-4765132 Msa0625590:intron 15.0%
!!! AAAAGTATTTTACAAGTATT+AGG + chr4_3:4765539-4765558 None:intergenic 15.0%
!!! TAATTTTAAAATGCATAAGT+TGG - chr4_3:4765642-4765661 Msa0625590:intron 15.0%
!!! TTCAAAAAAAATTTTTACCA+GGG + chr4_3:4765945-4765964 None:intergenic 15.0%
!!! ATTCAAAAAAAATTTTTACC+AGG + chr4_3:4765946-4765965 None:intergenic 15.0%
!!! AAAAAACAAAAAATTTTGCA+GGG + chr4_3:4765974-4765993 None:intergenic 15.0%
!!! TAAAAAACAAAAAATTTTGC+AGG + chr4_3:4765975-4765994 None:intergenic 15.0%
!! AGATCTTTGAATTAAAATTT+GGG + chr4_3:4766265-4766284 None:intergenic 15.0%
!!! TATTTTGTTTTTTCAGTTCA+TGG - chr4_3:4764054-4764073 Msa0625590:three_prime_UTR 20.0%
!!! ACAATCTTTTGATAAGTTTT+AGG - chr4_3:4764779-4764798 Msa0625590:intron 20.0%
!! AATATCTACGAAACAAAAAT+TGG - chr4_3:4764926-4764945 Msa0625590:intron 20.0%
!!! ATTTACATTTGCTATAAATG+TGG - chr4_3:4764996-4765015 Msa0625590:intron 20.0%
!!! GATCAAGTCAAATTTTTTAT+TGG - chr4_3:4765063-4765082 Msa0625590:intron 20.0%
!! TAAAATAATACAACCAACAA+TGG + chr4_3:4765292-4765311 None:intergenic 20.0%
!!! ATTAGGCTAAAATATTCTTT+TGG - chr4_3:4765887-4765906 Msa0625590:intron 20.0%
!! AATTAAAAAAAACTGCAGAA+AGG - chr4_3:4766176-4766195 Msa0625590:intron 20.0%
!! ATTAAAAAAAACTGCAGAAA+GGG - chr4_3:4766177-4766196 Msa0625590:intron 20.0%
!! GATCTTTGAATTAAAATTTG+GGG + chr4_3:4766264-4766283 None:intergenic 20.0%
!! CAGATCTTTGAATTAAAATT+TGG + chr4_3:4766266-4766285 None:intergenic 20.0%
!!! TAATTTTAAAATGCATAAGG+TGG - chr4_3:4766461-4766480 Msa0625590:intron 20.0%
!!! ATTTTAAACATCAAGATGAA+GGG + chr4_3:4767234-4767253 None:intergenic 20.0%
!!! TTATTAATTTTGTCAAGAGA+TGG - chr4_3:4767446-4767465 Msa0625590:five_prime_UTR 20.0%
!!! TATTAATTTTGTCAAGAGAT+GGG - chr4_3:4767447-4767466 Msa0625590:five_prime_UTR 20.0%
! TTTAGGAAGACAATAATGAT+TGG - chr4_3:4764159-4764178 Msa0625590:three_prime_UTR 25.0%
! AATGATTGGAAAAACAGATA+TGG - chr4_3:4764173-4764192 Msa0625590:three_prime_UTR 25.0%
! AATAATTAGTTAGGTGAAAG+TGG - chr4_3:4764476-4764495 Msa0625590:CDS 25.0%
! ATAATTAGTTAGGTGAAAGT+GGG - chr4_3:4764477-4764496 Msa0625590:CDS 25.0%
!! TAATTAGTTTCTGTTTGACT+AGG - chr4_3:4764698-4764717 Msa0625590:intron 25.0%
!!! ATAAGTTTTAGGCTGAATTT+AGG - chr4_3:4764790-4764809 Msa0625590:intron 25.0%
!!! TAAGTTTTAGGCTGAATTTA+GGG - chr4_3:4764791-4764810 Msa0625590:intron 25.0%
!!! GAGATTATAGTTGATAATGA+TGG - chr4_3:4765028-4765047 Msa0625590:intron 25.0%
! ATTATAAGTCTTTCACCATT+TGG - chr4_3:4765260-4765279 Msa0625590:intron 25.0%
! AAAATGTGAATAGATGATGT+GGG - chr4_3:4765451-4765470 Msa0625590:intron 25.0%
! TATACGCAATTAAATGACAT+TGG - chr4_3:4765506-4765525 Msa0625590:intron 25.0%
! TTCCCATGTATAAAAAAATG+TGG - chr4_3:4765842-4765861 Msa0625590:intron 25.0%
! TCCCATGTATAAAAAAATGT+GGG - chr4_3:4765843-4765862 Msa0625590:intron 25.0%
!!! TCCCACATTTTTTTATACAT+GGG + chr4_3:4765847-4765866 None:intergenic 25.0%
!!! TTCCCACATTTTTTTATACA+TGG + chr4_3:4765848-4765867 None:intergenic 25.0%
! GTGTCTTGTACATATAAATT+AGG - chr4_3:4765870-4765889 Msa0625590:intron 25.0%
!!! AATATAGTGAGTTTGAGTTT+TGG - chr4_3:4765918-4765937 Msa0625590:intron 25.0%
! TAAATGATATAATTGCCATG+TGG + chr4_3:4766087-4766106 None:intergenic 25.0%
! TTAAAAAAAACTGCAGAAAG+GGG - chr4_3:4766178-4766197 Msa0625590:intron 25.0%
!! TTTGTGAGATTCATTTTCAT+GGG + chr4_3:4766334-4766353 None:intergenic 25.0%
!! TTTTGTGAGATTCATTTTCA+TGG + chr4_3:4766335-4766354 None:intergenic 25.0%
! TTCCCATGTATAAAAAAATG+TGG - chr4_3:4765842-4765861 Msa0625590:intron 25.0%
! TCCCATGTATAAAAAAATGT+GGG - chr4_3:4765843-4765862 Msa0625590:intron 25.0%
!!! TCCCACATTTTTTTATACAT+GGG + chr4_3:4765847-4765866 None:intergenic 25.0%
!!! TTCCCACATTTTTTTATACA+TGG + chr4_3:4765848-4765867 None:intergenic 25.0%
! TCTTGTACGTACAAATTATA+TGG - chr4_3:4766688-4766707 Msa0625590:intron 25.0%
! AAGCAATGTTTATCTCAATA+AGG + chr4_3:4766946-4766965 None:intergenic 25.0%
! AACATTGCTTATATTATTGC+AGG - chr4_3:4766956-4766975 Msa0625590:intron 25.0%
!! TTTTAAACATCAAGATGAAG+GGG + chr4_3:4767233-4767252 None:intergenic 25.0%
!! GATTTTAAACATCAAGATGA+AGG + chr4_3:4767235-4767254 None:intergenic 25.0%
! GATAGCAACTATTGTAAAAT+TGG + chr4_3:4767291-4767310 None:intergenic 25.0%
! GATATAAAAAAAAACCAGCA+AGG - chr4_3:4767403-4767422 Msa0625590:five_prime_UTR 25.0%
!! ATTAATTTTGTCAAGAGATG+GGG - chr4_3:4767448-4767467 Msa0625590:five_prime_UTR 25.0%
! ATGGTGTTGAATATGCATTT+GGG - chr4_3:4764073-4764092 Msa0625590:three_prime_UTR 30.0%
GGAAAAACAGATATGGAAAT+TGG - chr4_3:4764180-4764199 Msa0625590:three_prime_UTR 30.0%
TGGAAATTCTATACCTAGTT+GGG - chr4_3:4764314-4764333 Msa0625590:CDS 30.0%
ACATGATCTATATCCAAAAC+AGG + chr4_3:4764426-4764445 None:intergenic 30.0%
!! TGTTTTGGATATAGATCATG+TGG - chr4_3:4764425-4764444 Msa0625590:CDS 30.0%
AAATGAAACACTGAGCAATT+TGG + chr4_3:4764567-4764586 None:intergenic 30.0%
AATCAAGATATCATAGTCGT+TGG + chr4_3:4764626-4764645 None:intergenic 30.0%
! ATTAGTCACTTTCACAGATT+TGG + chr4_3:4764682-4764701 None:intergenic 30.0%
CTTGATCAAAATCTTCACAA+CGG + chr4_3:4764968-4764987 None:intergenic 30.0%
TAATACAACCAACAATGGAT+TGG + chr4_3:4765287-4765306 None:intergenic 30.0%
ATTAAGCAAATAGTCTCCAA+TGG + chr4_3:4765396-4765415 None:intergenic 30.0%
GAAAATGTGAATAGATGATG+TGG - chr4_3:4765450-4765469 Msa0625590:intron 30.0%
TCAAACTCACTATATTTGCA+GGG + chr4_3:4765914-4765933 None:intergenic 30.0%
!!! TTGTTTTTTAAAATGGTCCC+TGG - chr4_3:4765985-4766004 Msa0625590:intron 30.0%
!!! TGTTTTTTAAAATGGTCCCT+GGG - chr4_3:4765986-4766005 Msa0625590:intron 30.0%
TGACAAATCAGCAAAAAAGT+GGG + chr4_3:4766012-4766031 None:intergenic 30.0%
TAAAAAAAACTGCAGAAAGG+GGG - chr4_3:4766179-4766198 Msa0625590:intron 30.0%
ACATATCAACTGCTAAGAAA+TGG + chr4_3:4766769-4766788 None:intergenic 30.0%
! GAACTTTTATGCTAAACTTC+CGG - chr4_3:4766903-4766922 Msa0625590:intron 30.0%
GGTGATAGATATCTTAATTC+CGG + chr4_3:4766925-4766944 None:intergenic 30.0%
! TTTTGTTGAGTTCAATGTCA+CGG + chr4_3:4767005-4767024 None:intergenic 30.0%
ATTAACAAGTGAACCAAACA+AGG - chr4_3:4767090-4767109 Msa0625590:intron 30.0%
CTTGAAACATTAACCTTGTT+TGG + chr4_3:4767106-4767125 None:intergenic 30.0%
ATTCAATATGTTGCCTTTCT+TGG + chr4_3:4767320-4767339 None:intergenic 30.0%
! CATGGTGTTGAATATGCATT+TGG - chr4_3:4764072-4764091 Msa0625590:three_prime_UTR 35.0%
! TGGTGTTGAATATGCATTTG+GGG - chr4_3:4764074-4764093 Msa0625590:three_prime_UTR 35.0%
TGGTTCTCCTTCAAATATAC+CGG + chr4_3:4764118-4764137 None:intergenic 35.0%
GTATATTTGAAGGAGAACCA+AGG - chr4_3:4764118-4764137 Msa0625590:three_prime_UTR 35.0%
AATGTGAAGGGTTCAGATTT+AGG - chr4_3:4764142-4764161 Msa0625590:three_prime_UTR 35.0%
CACAAGCATCATTGCAAAAA+TGG + chr4_3:4764284-4764303 None:intergenic 35.0%
CTGGAAATTCTATACCTAGT+TGG - chr4_3:4764313-4764332 Msa0625590:CDS 35.0%
GTTAATCGTCTTGCTAGAAT+TGG - chr4_3:4764336-4764355 Msa0625590:CDS 35.0%
GAGTAGTAACAACAACTAGT+AGG + chr4_3:4764375-4764394 None:intergenic 35.0%
! GGGATATAAGAACCTTTTCT+CGG - chr4_3:4764811-4764830 Msa0625590:intron 35.0%
! TAAGAACCTTTTCTCGGAAT+AGG - chr4_3:4764817-4764836 Msa0625590:intron 35.0%
AACTTTCCTATTCCGAGAAA+AGG + chr4_3:4764826-4764845 None:intergenic 35.0%
AATCCTTAAACACGTGATCA+GGG + chr4_3:4765243-4765262 None:intergenic 35.0%
TAATCCTTAAACACGTGATC+AGG + chr4_3:4765244-4765263 None:intergenic 35.0%
ATCAAAATCCTCAAGACCAT+TGG - chr4_3:4765377-4765396 Msa0625590:intron 35.0%
! TGCTACACGTTGAATTTGTT+CGG - chr4_3:4765415-4765434 Msa0625590:intron 35.0%
!! GGGAGAGTTACTTTAGAATT+AGG - chr4_3:4765471-4765490 Msa0625590:intron 35.0%
CTCAAACTCACTATATTTGC+AGG + chr4_3:4765915-4765934 None:intergenic 35.0%
GTGACAAATCAGCAAAAAAG+TGG + chr4_3:4766013-4766032 None:intergenic 35.0%
ATGATATAATTGCCATGTGG+AGG + chr4_3:4766084-4766103 None:intergenic 35.0%
ATCTACAATCTCTAACACAC+AGG - chr4_3:4766287-4766306 Msa0625590:intron 35.0%
AAGTGAATGCCTTTCTTTAG+TGG + chr4_3:4766433-4766452 None:intergenic 35.0%
! TTCTTAGCAGTTGATATGTG+TGG - chr4_3:4766770-4766789 Msa0625590:intron 35.0%
TTTGTTGAGTTCAATGTCAC+GGG + chr4_3:4767004-4767023 None:intergenic 35.0%
TGCTATCAACAAACCAAGAA+AGG - chr4_3:4767304-4767323 Msa0625590:CDS 35.0%
ATTAGTACATGATTCCTTGC+TGG + chr4_3:4767420-4767439 None:intergenic 35.0%
GCATCAACCGGTATATTTGA+AGG - chr4_3:4764108-4764127 Msa0625590:three_prime_UTR 40.0%
GAGAACCAAGGAAATGTGAA+GGG - chr4_3:4764130-4764149 Msa0625590:three_prime_UTR 40.0%
! TGGTGAAGTGAGAAGTGTAA+TGG - chr4_3:4764200-4764219 Msa0625590:three_prime_UTR 40.0%
GATGCTTGTGTTAAGCTTAC+TGG - chr4_3:4764294-4764313 Msa0625590:CDS 40.0%
ATCCTTAAACACGTGATCAG+GGG + chr4_3:4765242-4765261 None:intergenic 40.0%
!! CAACAATGGATTGGTCCAAA+TGG + chr4_3:4765278-4765297 None:intergenic 40.0%
CATTTGGACCAATCCATTGT+TGG - chr4_3:4765276-4765295 Msa0625590:intron 40.0%
! AATAGTCTCCAATGGTCTTG+AGG + chr4_3:4765388-4765407 None:intergenic 40.0%
!! TTTTCATGGGTGTGGTTTTG+AGG + chr4_3:4766321-4766340 None:intergenic 40.0%
! GAGATTCATTTTCATGGGTG+TGG + chr4_3:4766329-4766348 None:intergenic 40.0%
GATTTGTGAGTGTGCAGATT+TGG + chr4_3:4766379-4766398 None:intergenic 40.0%
!!! CTGGGTTTTGGGTTTTGATA+TGG + chr4_3:4766408-4766427 None:intergenic 40.0%
ACATTTGTCATGTGTGACGT+AGG - chr4_3:4766726-4766745 Msa0625590:intron 40.0%
!! CTTCTAGCTCTGGATTAAGA+CGG - chr4_3:4767158-4767177 Msa0625590:intron 40.0%
!! TTCTAGCTCTGGATTAAGAC+GGG - chr4_3:4767159-4767178 Msa0625590:intron 40.0%
ATAATCAAAGGTGAAGGTGG+AGG + chr4_3:4767205-4767224 None:intergenic 40.0%
GCAATAATCAAAGGTGAAGG+TGG + chr4_3:4767208-4767227 None:intergenic 40.0%
GAGGCAATAATCAAAGGTGA+AGG + chr4_3:4767211-4767230 None:intergenic 40.0%
TAAACATCAAGATGAAGGGG+AGG + chr4_3:4767230-4767249 None:intergenic 40.0%
GCACATGAGTATGCATCAAC+CGG - chr4_3:4764096-4764115 Msa0625590:three_prime_UTR 45.0%
GGAGAACCAAGGAAATGTGA+AGG - chr4_3:4764129-4764148 Msa0625590:three_prime_UTR 45.0%
CTGAACCCTTCACATTTCCT+TGG + chr4_3:4764138-4764157 None:intergenic 45.0%
!! GAACTTGCTGCTGAGTACAA+AGG - chr4_3:4764225-4764244 Msa0625590:three_prime_UTR 45.0%
GCAAGACGATTAACCCAACT+AGG + chr4_3:4764330-4764349 None:intergenic 45.0%
AAGTGGGAGCAACATGTAAG+CGG - chr4_3:4764493-4764512 Msa0625590:CDS 45.0%
GAGCAACATGTAAGCGGTAA+CGG - chr4_3:4764499-4764518 Msa0625590:CDS 45.0%
! TGTAAGCGGTAACGGTACAT+CGG - chr4_3:4764507-4764526 Msa0625590:CDS 45.0%
CATAGTCGTTGGATCGAGAT+TGG + chr4_3:4764615-4764634 None:intergenic 45.0%
! AACACGTGATCAGGGGTTTT+TGG + chr4_3:4765235-4765254 None:intergenic 45.0%
AACCCCTGATCACGTGTTTA+AGG - chr4_3:4765237-4765256 Msa0625590:intron 45.0%
!! TGAGTTTGAGTTTTGGTCCC+TGG - chr4_3:4765925-4765944 Msa0625590:intron 45.0%
TTGTCACATTTCTGCCTACG+TGG - chr4_3:4766026-4766045 Msa0625590:intron 45.0%
!! CTTTAGTGGCACTGGGTTTT+GGG + chr4_3:4766419-4766438 None:intergenic 45.0%
!! TCTTTAGTGGCACTGGGTTT+TGG + chr4_3:4766420-4766439 None:intergenic 45.0%
! AACCCAGTGCCACTAAAGAA+AGG - chr4_3:4766421-4766440 Msa0625590:intron 45.0%
TGCCTTTCTTTAGTGGCACT+GGG + chr4_3:4766426-4766445 None:intergenic 45.0%
ATGCCTTTCTTTAGTGGCAC+TGG + chr4_3:4766427-4766446 None:intergenic 45.0%
!! ACCTTCAAGAGCATCTTCAC+CGG - chr4_3:4767132-4767151 Msa0625590:intron 45.0%
AATCCAGAGCTAGAAGAAGC+CGG + chr4_3:4767154-4767173 None:intergenic 45.0%
!! TCTAGCTCTGGATTAAGACG+GGG - chr4_3:4767160-4767179 Msa0625590:intron 45.0%
GAAGGGGAGGCAATAATCAA+AGG + chr4_3:4767217-4767236 None:intergenic 45.0%
!!! AAATTTTTTGTTTTTTAAAA+TGG - chr4_3:4765978-4765997 Msa0625590:intron 5.0%
CGTCTAATCTCGACCCAAGT+GGG + chr4_3:4764542-4764561 None:intergenic 50.0%
CCACTTGGGTCGAGATTAGA+CGG - chr4_3:4764540-4764559 Msa0625590:intron 50.0%
TCAGCAAAAAAGTGGGCCCA+GGG + chr4_3:4766005-4766024 None:intergenic 50.0%
ATCAGCAAAAAAGTGGGCCC+AGG + chr4_3:4766006-4766025 None:intergenic 50.0%
TTGCCATGTGGAGGACATGT+GGG + chr4_3:4766075-4766094 None:intergenic 50.0%
ATTGCCATGTGGAGGACATG+TGG + chr4_3:4766076-4766095 None:intergenic 50.0%
! AAGCGGTAACGGTACATCGG+AGG - chr4_3:4764510-4764529 Msa0625590:CDS 55.0%
ATCGGAGGTTGTTGCCCACT+TGG - chr4_3:4764525-4764544 Msa0625590:intron 55.0%
TCGGAGGTTGTTGCCCACTT+GGG - chr4_3:4764526-4764545 Msa0625590:intron 55.0%
CCGTCTAATCTCGACCCAAG+TGG + chr4_3:4764543-4764562 None:intergenic 55.0%
ACAGTCAGCAACTGCCACGT+AGG + chr4_3:4766043-4766062 None:intergenic 55.0%
TTGCCCACATGTCCTCCACA+TGG - chr4_3:4766069-4766088 Msa0625590:intron 55.0%
GCCGGTGAAGATGCTCTTGA+AGG + chr4_3:4767136-4767155 None:intergenic 55.0%
!! TCACCGGCTTCTTCTAGCTC+TGG - chr4_3:4767148-4767167 Msa0625590:intron 55.0%
! GCTCTGGATTAAGACGGGGA+AGG - chr4_3:4767164-4767183 Msa0625590:CDS 55.0%
GATTAAGACGGGGAAGGAGC+AGG - chr4_3:4767170-4767189 Msa0625590:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 4764043 4767475 4764043 ID=Msa0625590;Name=Msa0625590
chr4_3 mRNA 4764043 4767475 4764043 ID=Msa0625590-mRNA-1;Parent=Msa0625590;Name=Msa0625590-mRNA-1;_AED=0.02;_eAED=0.02;_QI=131|1|1|1|1|1|2|232|149
chr4_3 exon 4764043 4764540 4764043 ID=Msa0625590-mRNA-1:exon:1316;Parent=Msa0625590-mRNA-1
chr4_3 exon 4767161 4767475 4767161 ID=Msa0625590-mRNA-1:exon:1315;Parent=Msa0625590-mRNA-1
chr4_3 five_prime_UTR 4767345 4767475 4767345 ID=Msa0625590-mRNA-1:five_prime_utr;Parent=Msa0625590-mRNA-1
chr4_3 CDS 4767161 4767344 4767161 ID=Msa0625590-mRNA-1:cds;Parent=Msa0625590-mRNA-1
chr4_3 CDS 4764275 4764540 4764275 ID=Msa0625590-mRNA-1:cds;Parent=Msa0625590-mRNA-1
chr4_3 three_prime_UTR 4764043 4764274 4764043 ID=Msa0625590-mRNA-1:three_prime_utr;Parent=Msa0625590-mRNA-1
Gene Sequence

>Msa0625590

ATGATTGGAAAAACAGATATGGAAATTGGTGAAGTGAGAAGTGTAATGGAAGAACTTGCTGCTGAGTACAAAGGAAATGCTTACAATCTCATTACAAAAAATTGTAACCATTTTTGCAATGATGCTTGTGTTAAGCTTACTGGAAATTCTATACCTAGTTGGGTTAATCGTCTTGCTAGAATTGGTTTTATGTGCAATTGTGTTCTTCCCGTGACATTGAACTCAACAAAAGTTAGACATCATCATCATAGAATAGAAGACAAACAATGTGACGATGATAAACAACTCAAACAAGCATTAACAAGTGAACCAAACAAGGTTAATGTTTCAAGTTCAACACCTTCAAGAGCATCTTCACCGGCTTCTTCTAGCTCTGGATTAAGACGGGGAAGGAGCAGGACAAGACGTCCTCCACCTTCACCTTTGATTATTGCCTCCCCTTCATCTTGA
Protein sequence

>Msa0625590

MIGKTDMEIGEVRSVMEELAAEYKGNAYNLITKNCNHFCNDACVKLTGNSIPSWVNRLARIGFMCNCVLPVTLNSTKVRHHHHRIEDKQCDDDKQLKQALTSEPNKVNVSSSTPSRASSPASSSSGLRRGRSRTRRPPPSPLIIASPSS*