AlfalfaGEDB | Msa0631280

M. sativa cultivar zhongmu-4 / Msa0631280

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0631280	XP_013454925.1	92.949	156	10	1	1	155	1	156	2.01e-96	285

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0631280	A0A072UIR5	92.949	156	10	1	1	155	1	156	9.60e-97	285

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0534790	Msa0631280	0.902638	7.898752e-79	-8.615850e-47
Msa0590970	Msa0631280	0.871997	4.459882e-67	-8.615850e-47
Msa0631280	Msa0669550	0.881104	3.165558e-70	-8.615850e-47
Msa0631280	Msa0905260	0.813747	2.188491e-51	-8.615850e-47
Msa0631280	Msa0974530	0.817720	2.849244e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0631280	MtrunA17_Chr4g0008731	92.949	156	10	1	1	155	1	156	1.85e-100	285
Msa0631280	MtrunA17_Chr7g0240431	53.623	138	62	2	18	155	21	156	1.18e-46	149
Msa0631280	MtrunA17_Chr4g0008721	50.000	158	69	5	1	155	1	151	2.08e-38	128

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0631280	AT5G02020.1	50.360	139	58	4	17	155	22	149	4.05e-32	112
Msa0631280	AT5G02020.2	49.153	118	51	4	17	133	22	131	1.82e-20	82.4
Msa0631280	AT2G39855.2	32.374	139	76	3	18	155	21	142	9.70e-14	65.1
Msa0631280	AT2G39855.4	32.374	139	76	3	18	155	21	142	9.70e-14	65.1
Msa0631280	AT5G59080.2	32.414	145	65	3	16	155	19	135	6.60e-13	62.8
Msa0631280	AT5G59080.1	32.414	145	65	3	16	155	19	135	6.60e-13	62.8
Msa0631280	AT3G55646.1	30.952	126	69	2	30	155	39	146	3.52e-11	58.5
Msa0631280	AT3G46880.2	37.647	85	52	1	72	155	32	116	5.89e-11	57.0
Msa0631280	AT2G39855.3	46.032	63	28	2	94	155	60	117	7.43e-11	57.0

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Find 32 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGGCTACAAGAGGGGAC+TGG	0.623507	4_3:+14070887	Msa0631280:CDS
AACACCAAAACTGTTACTCA+AGG	0.603702	4_3:+14069982	Msa0631280:CDS
AACTTATAGCACAAGAATCC+TGG	0.444574	4_3:+14070847	Msa0631280:intron
ACAAGAGGGGACTGGTGGCA+GGG	0.569843	4_3:+14070895	Msa0631280:CDS
AGCATCTTTAAAGACAATGG+TGG	0.682264	4_3:+14070422	Msa0631280:CDS
AGGAGAAGATGATTCCCAAA+TGG	0.568509	4_3:+14070870	Msa0631280:CDS
AGTACCTTGAGTAACAGTTT+TGG	0.440213	4_3:-14069986	None:intergenic
AGTCCCCTCTTGTAGCCATT+TGG	0.351874	4_3:-14070885	None:intergenic
AGTTCATCAATCTATTATGG+TGG	0.597056	4_3:+14070518	Msa0631280:CDS
ATTCCCAAATGGCTACAAGA+GGG	0.639337	4_3:+14070881	Msa0631280:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGAAGGAAAAAATAAGCAAA+AGG	+	chr4_3:14069339-14069358	Msa0631280:CDS	30.0%
AGGTGATTGAGTAGAAAAAA+TGG	-	chr4_3:14069446-14069465	None:intergenic	30.0%
ATAACCAGATGAAGGAAATA+AGG	-	chr4_3:14069784-14069803	None:intergenic	30.0%
TACATTGTACCTAGAGATTT+AGG	-	chr4_3:14070292-14070311	None:intergenic	30.0%
AGTTCATCAATCTATTATGG+TGG	+	chr4_3:14070518-14070537	Msa0631280:CDS	30.0%
TGTTTATATTTGACACCAGA+AGG	+	chr4_3:14070761-14070780	Msa0631280:intron	30.0%
GGAAGGAAAAAATAAGCAAA+AGG	+	chr4_3:14069339-14069358	Msa0631280:CDS	30.0%
AGGTGATTGAGTAGAAAAAA+TGG	-	chr4_3:14069446-14069465	None:intergenic	30.0%
ATAACCAGATGAAGGAAATA+AGG	-	chr4_3:14069784-14069803	None:intergenic	30.0%
TACATTGTACCTAGAGATTT+AGG	-	chr4_3:14070292-14070311	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	14069337	14071291	14069337	ID=Msa0631280;Name=Msa0631280
chr4_3	mRNA	14069337	14071291	14069337	ID=Msa0631280-mRNA-1;Parent=Msa0631280;Name=Msa0631280-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0.75\|0.4\|0.8\|1\|1\|5\|0\|167
chr4_3	exon	14069337	14069468	14069337	ID=Msa0631280-mRNA-1:exon:3921;Parent=Msa0631280-mRNA-1
chr4_3	exon	14069922	14070003	14069922	ID=Msa0631280-mRNA-1:exon:3922;Parent=Msa0631280-mRNA-1
chr4_3	exon	14070420	14070595	14070420	ID=Msa0631280-mRNA-1:exon:3923;Parent=Msa0631280-mRNA-1
chr4_3	exon	14070856	14070916	14070856	ID=Msa0631280-mRNA-1:exon:3924;Parent=Msa0631280-mRNA-1
chr4_3	exon	14071239	14071291	14071239	ID=Msa0631280-mRNA-1:exon:3925;Parent=Msa0631280-mRNA-1
chr4_3	CDS	14069337	14069468	14069337	ID=Msa0631280-mRNA-1:cds;Parent=Msa0631280-mRNA-1
chr4_3	CDS	14069922	14070003	14069922	ID=Msa0631280-mRNA-1:cds;Parent=Msa0631280-mRNA-1
chr4_3	CDS	14070420	14070595	14070420	ID=Msa0631280-mRNA-1:cds;Parent=Msa0631280-mRNA-1
chr4_3	CDS	14070856	14070916	14070856	ID=Msa0631280-mRNA-1:cds;Parent=Msa0631280-mRNA-1
chr4_3	CDS	14071239	14071291	14071239	ID=Msa0631280-mRNA-1:cds;Parent=Msa0631280-mRNA-1

Gene Sequence

>Msa0631280

ATGGAAGGAAAAAATAAGCAAAAGGGTATTTCTTCTTCACCTTGTTCTGAGCTCTTTGGGTCTAAACAATGTCACTCATCATCTTCTTCTTCTGGAATTTATGGTTCCATTTTTTCTACTCAATCACCTAAGATTTTAGGGAGACAATCTGTGCGATCTGAAGTGAGTGGCAAAACAACAAATGATACATTGAACACCAAAACTGTTACTCAAGATAGCATCTTTAAAGACAATGGTGGTGAAGCTCACGAGACAAAAAAGACTGATATGAGTTTTCTCTATAAGGGTCAAAGAGCTCAACCATGTCACCTTAGTTCATCAATCTATTATGGTGGCCAAGACATATATCCTCAACCTCAAAGTACTCAAAATGCTGCATTCAACTCCAAACACAAGAATCCTGGAGGAGAAGATGATTCCCAAATGGCTACAAGAGGGGACTGGTGGCAGGGTACATGGCATTATAGTCAATCTAGTTACATGGATGAACTAAAGAGGAGATAG

Protein sequence

>Msa0631280

MEGKNKQKGISSSPCSELFGSKQCHSSSSSSGIYGSIFSTQSPKILGRQSVRSEVSGKTTNDTLNTKTVTQDSIFKDNGGEAHETKKTDMSFLYKGQRAQPCHLSSSIYYGGQDIYPQPQSTQNAAFNSKHKNPGGEDDSQMATRGDWWQGTWHYSQSSYMDELKRR*