AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0648320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0648320 MtrunA17_Chr4g0040201 94.231 208 11 1 1 207 1 208 8.64e-144 399
Msa0648320 MtrunA17_Chr2g0324511 65.409 159 45 5 8 160 9 163 5.56e-60 185
Msa0648320 MtrunA17_Chr2g0324521 59.483 116 44 2 47 160 2 116 4.87e-39 130
Msa0648320 MtrunA17_Chr2g0324571 57.500 120 42 3 47 160 2 118 4.70e-38 128
Msa0648320 MtrunA17_Chr2g0282341 44.526 137 63 6 50 180 4 133 5.29e-29 105
Msa0648320 MtrunA17_Chr2g0324531 60.465 43 17 0 159 201 2 44 1.01e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0648320 AT4G20970.1 42.328 189 89 8 8 180 2 186 2.11e-32 116
Msa0648320 AT1G10585.1 27.429 175 116 6 10 182 10 175 1.79e-11 60.8

Find 59 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTTAGAAATTACTTAAA+AGG 0.118257 4_3:-54822173 Msa0648320:five_prime_UTR
ATTTCAAAACATGGTGATTT+TGG 0.176377 4_3:+54821677 None:intergenic
CTAATATTTATATCTAATTA+TGG 0.230205 4_3:-54822124 Msa0648320:exon
TTAGGTCTATCTTAATATAT+AGG 0.260682 4_3:-54821246 Msa0648320:three_prime_UTR
AGAACCTTGGGGTTATAGTT+AGG 0.280194 4_3:+54821982 None:intergenic
TATCTAATTATGGATCAAAT+TGG 0.283616 4_3:-54822114 Msa0648320:exon
AGGGAGAAGAGAGTTGAGTT+TGG 0.296752 4_3:+54822002 None:intergenic
ATAAGAATTATGCTAGATTA+AGG 0.327945 4_3:-54821045 Msa0648320:three_prime_UTR
TAGGTCTATCTTAATATATA+GGG 0.337165 4_3:-54821245 Msa0648320:three_prime_UTR
AATATGTGGGGTTTCTAGTT+AGG 0.344816 4_3:-54821264 Msa0648320:exon
TACAAGACGATTAACTTATC+AGG 0.347401 4_3:+54821020 None:intergenic
ATATTAAGCTAAGAGATTAA+AGG 0.355914 4_3:+54821075 None:intergenic
ATCTTAGAAATTACTTAAAA+GGG 0.370468 4_3:-54822172 Msa0648320:five_prime_UTR
GAGAGTTGAGTTTGGAGTAG+AGG 0.397503 4_3:+54822010 None:intergenic
TGAATTATCTCCAACCTTAG+AGG 0.408147 4_3:+54821535 None:intergenic
GGAATTACCGTTGGCGCTAC+CGG 0.409114 4_3:-54821825 Msa0648320:CDS
AACCAGCCTTCTTTCAACTT+TGG 0.410390 4_3:+54822062 None:intergenic
GAACCTTGGGGTTATAGTTA+GGG 0.410715 4_3:+54821983 None:intergenic
TGATTTCATAGAAAATGAAT+TGG 0.411346 4_3:+54821603 None:intergenic
AAGGAGTGTCAATTCCTCTA+AGG 0.421100 4_3:-54821549 Msa0648320:CDS
ATCGCCTCATCTACTTGATC+CGG 0.457881 4_3:+54821806 None:intergenic
TATGTAGGTAGGTTAAGAAT+AGG 0.460145 4_3:+54822216 None:intergenic
TGGCTAAGGAGAAGAAAGAA+AGG 0.476291 4_3:-54821748 Msa0648320:CDS
AAGTGAGTGAGAGATTGAAA+AGG 0.491808 4_3:-54821359 Msa0648320:CDS
GCTAGGGGATATAGCTATGT+AGG 0.500307 4_3:+54822201 None:intergenic
TTGGTTGTATTAGAGCTAGA+AGG 0.500610 4_3:+54822081 None:intergenic
ATGAAGAGAATGTAGACATA+AGG 0.503791 4_3:-54821568 Msa0648320:CDS
ATTTCTAAGATGAACTAGCT+AGG 0.508786 4_3:+54822184 None:intergenic
AGATTGAAAAGGTTTGTGTA+TGG 0.509660 4_3:-54821348 Msa0648320:CDS
TTTCTAAGATGAACTAGCTA+GGG 0.509844 4_3:+54822185 None:intergenic
AGATCACGTGAATATTGCTT+AGG 0.510152 4_3:-54821704 Msa0648320:CDS
TTGGAACTGATCATAATATG+TGG 0.512688 4_3:-54821278 Msa0648320:CDS
CTAAAGTTGTGATTGATTGT+CGG 0.519208 4_3:-54821131 Msa0648320:three_prime_UTR
AGGCTGTATTTCCACATCAC+TGG 0.522661 4_3:+54821320 None:intergenic
ATACAACCAAAGTTGAAAGA+AGG 0.526780 4_3:-54822068 Msa0648320:CDS
TATAATTCAGGAATTACCGT+TGG 0.530375 4_3:-54821834 Msa0648320:intron
ACTTGATCCGGTAGCGCCAA+CGG 0.534008 4_3:+54821818 None:intergenic
ATTTCTTGAATTTCAAAACA+TGG 0.541430 4_3:+54821668 None:intergenic
CCTTCATGTTGTTCATGCTG+AGG 0.541669 4_3:-54821510 Msa0648320:intron
AACCAAAGTTGAAAGAAGGC+TGG 0.543583 4_3:-54822064 Msa0648320:CDS
TTTAGAGGCAAATGTTGAGA+TGG 0.546113 4_3:-54821768 Msa0648320:CDS
TACAAATCATTCTCACGTGT+GGG 0.548323 4_3:-54821634 Msa0648320:CDS
TGGAACTGATCATAATATGT+GGG 0.553773 4_3:-54821277 Msa0648320:CDS
CTACAAATCATTCTCACGTG+TGG 0.558837 4_3:-54821635 Msa0648320:CDS
AGTGTCAATTCCTCTAAGGT+TGG 0.561526 4_3:-54821545 Msa0648320:CDS
ATTTCAAAATCCCACAACTC+AGG 0.570407 4_3:+54821300 None:intergenic
GGAAATACAGCCTGAGTTGT+GGG 0.582012 4_3:-54821310 Msa0648320:CDS
TCTTAGAAATTACTTAAAAG+GGG 0.582074 4_3:-54822171 Msa0648320:five_prime_UTR
GGGGATATAGCTATGTAGGT+AGG 0.585601 4_3:+54822205 None:intergenic
GCTCCAAATTGAAAACAAGA+TGG 0.586259 4_3:+54821387 None:intergenic
TGGAAATACAGCCTGAGTTG+TGG 0.593486 4_3:-54821311 Msa0648320:CDS
GGCAAATGTTGAGATGGCTA+AGG 0.613070 4_3:-54821762 Msa0648320:CDS
CCTCAGCATGAACAACATGA+AGG 0.636646 4_3:+54821510 None:intergenic
CCAAATTGAAAACAAGATGG+AGG 0.639764 4_3:+54821390 None:intergenic
GGAACTGATCATAATATGTG+GGG 0.640483 4_3:-54821276 Msa0648320:CDS
GTATGGATCTTCCAGTGATG+TGG 0.643608 4_3:-54821331 Msa0648320:CDS
TTCTAAGATGAACTAGCTAG+GGG 0.654537 4_3:+54822186 None:intergenic
TCTCCCTAACTATAACCCCA+AGG 0.657270 4_3:-54821986 Msa0648320:intron
GCTACCGGATCAAGTAGATG+AGG 0.685176 4_3:-54821810 Msa0648320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATATTTATATCTAATTA+TGG - chr4_3:54821131-54821150 Msa0648320:three_prime_UTR 10.0%
!! CTAATATTTATATCTAATTA+TGG - chr4_3:54821131-54821150 Msa0648320:three_prime_UTR 10.0%
!! ATCTTAGAAATTACTTAAAA+GGG - chr4_3:54821083-54821102 Msa0648320:three_prime_UTR 15.0%
!! AAAAAAAAAGTTAGTTACAA+TGG - chr4_3:54821364-54821383 Msa0648320:CDS 15.0%
!!! GATGTAATTTTTTATAATTC+AGG - chr4_3:54821409-54821428 Msa0648320:intron 15.0%
!!! TTTATCTTTTACAAAACTTT+TGG + chr4_3:54822039-54822058 None:intergenic 15.0%
!!! TTTTACATTGAAGTTATATA+CGG - chr4_3:54822157-54822176 Msa0648320:five_prime_UTR 15.0%
!! ATCTTAGAAATTACTTAAAA+GGG - chr4_3:54821083-54821102 Msa0648320:three_prime_UTR 15.0%
!! AAAAAAAAAGTTAGTTACAA+TGG - chr4_3:54821364-54821383 Msa0648320:CDS 15.0%
!!! GATGTAATTTTTTATAATTC+AGG - chr4_3:54821409-54821428 Msa0648320:intron 15.0%
!!! TTTATCTTTTACAAAACTTT+TGG + chr4_3:54822039-54822058 None:intergenic 15.0%
!!! TTTTACATTGAAGTTATATA+CGG - chr4_3:54822157-54822176 Msa0648320:five_prime_UTR 15.0%
!! CATCTTAGAAATTACTTAAA+AGG - chr4_3:54821082-54821101 Msa0648320:three_prime_UTR 20.0%
!! TCTTAGAAATTACTTAAAAG+GGG - chr4_3:54821084-54821103 Msa0648320:three_prime_UTR 20.0%
!! TATCTAATTATGGATCAAAT+TGG - chr4_3:54821141-54821160 Msa0648320:three_prime_UTR 20.0%
!! AAACTACATAAAAAGTTTAG+AGG - chr4_3:54821472-54821491 Msa0648320:intron 20.0%
!! ATTTCTTGAATTTCAAAACA+TGG + chr4_3:54821590-54821609 None:intergenic 20.0%
!!! TGTTTTGAAATTCAAGAAAT+TGG - chr4_3:54821590-54821609 Msa0648320:CDS 20.0%
!! TGATTTCATAGAAAATGAAT+TGG + chr4_3:54821655-54821674 None:intergenic 20.0%
!! TTAGGTCTATCTTAATATAT+AGG - chr4_3:54822009-54822028 Msa0648320:CDS 20.0%
!! TAGGTCTATCTTAATATATA+GGG - chr4_3:54822010-54822029 Msa0648320:CDS 20.0%
!! ATATTAAGCTAAGAGATTAA+AGG + chr4_3:54822183-54822202 None:intergenic 20.0%
!! ATAAGAATTATGCTAGATTA+AGG - chr4_3:54822210-54822229 Msa0648320:five_prime_UTR 20.0%
!! CATCTTAGAAATTACTTAAA+AGG - chr4_3:54821082-54821101 Msa0648320:three_prime_UTR 20.0%
!! TCTTAGAAATTACTTAAAAG+GGG - chr4_3:54821084-54821103 Msa0648320:three_prime_UTR 20.0%
!! TATCTAATTATGGATCAAAT+TGG - chr4_3:54821141-54821160 Msa0648320:three_prime_UTR 20.0%
!! AAACTACATAAAAAGTTTAG+AGG - chr4_3:54821472-54821491 Msa0648320:intron 20.0%
!! ATTTCTTGAATTTCAAAACA+TGG + chr4_3:54821590-54821609 None:intergenic 20.0%
!!! TGTTTTGAAATTCAAGAAAT+TGG - chr4_3:54821590-54821609 Msa0648320:CDS 20.0%
!! TGATTTCATAGAAAATGAAT+TGG + chr4_3:54821655-54821674 None:intergenic 20.0%
!! TTAGGTCTATCTTAATATAT+AGG - chr4_3:54822009-54822028 Msa0648320:CDS 20.0%
!! TAGGTCTATCTTAATATATA+GGG - chr4_3:54822010-54822029 Msa0648320:CDS 20.0%
!! ATATTAAGCTAAGAGATTAA+AGG + chr4_3:54822183-54822202 None:intergenic 20.0%
!! ATAAGAATTATGCTAGATTA+AGG - chr4_3:54822210-54822229 Msa0648320:five_prime_UTR 20.0%
! AAGAAAGAAATTAGAACCTT+GGG + chr4_3:54821288-54821307 None:intergenic 25.0%
! AAAGAAAGAAATTAGAACCT+TGG + chr4_3:54821289-54821308 None:intergenic 25.0%
!! ATTTCAAAACATGGTGATTT+TGG + chr4_3:54821581-54821600 None:intergenic 25.0%
! AAGAAAGAAATTAGAACCTT+GGG + chr4_3:54821288-54821307 None:intergenic 25.0%
! AAAGAAAGAAATTAGAACCT+TGG + chr4_3:54821289-54821308 None:intergenic 25.0%
!! ATTTCAAAACATGGTGATTT+TGG + chr4_3:54821581-54821600 None:intergenic 25.0%
TATGTAGGTAGGTTAAGAAT+AGG + chr4_3:54821042-54821061 None:intergenic 30.0%
!! TTTCTAAGATGAACTAGCTA+GGG + chr4_3:54821073-54821092 None:intergenic 30.0%
!! ATTTCTAAGATGAACTAGCT+AGG + chr4_3:54821074-54821093 None:intergenic 30.0%
ATACAACCAAAGTTGAAAGA+AGG - chr4_3:54821187-54821206 Msa0648320:three_prime_UTR 30.0%
AGAAAGAAATTAGAACCTTG+GGG + chr4_3:54821287-54821306 None:intergenic 30.0%
ACAATACTAGTTAGCTACAA+TGG - chr4_3:54821330-54821349 Msa0648320:CDS 30.0%
TATAATTCAGGAATTACCGT+TGG - chr4_3:54821421-54821440 Msa0648320:intron 30.0%
ATGAAGAGAATGTAGACATA+AGG - chr4_3:54821687-54821706 Msa0648320:CDS 30.0%
ATAAAGAGAGAGCAATTACT+AGG + chr4_3:54821838-54821857 None:intergenic 30.0%
AGATTGAAAAGGTTTGTGTA+TGG - chr4_3:54821907-54821926 Msa0648320:intron 30.0%
TTGGAACTGATCATAATATG+TGG - chr4_3:54821977-54821996 Msa0648320:intron 30.0%
TGGAACTGATCATAATATGT+GGG - chr4_3:54821978-54821997 Msa0648320:intron 30.0%
CTAAAGTTGTGATTGATTGT+CGG - chr4_3:54822124-54822143 Msa0648320:exon 30.0%
TATGTAGGTAGGTTAAGAAT+AGG + chr4_3:54821042-54821061 None:intergenic 30.0%
!! TTTCTAAGATGAACTAGCTA+GGG + chr4_3:54821073-54821092 None:intergenic 30.0%
!! ATTTCTAAGATGAACTAGCT+AGG + chr4_3:54821074-54821093 None:intergenic 30.0%
ATACAACCAAAGTTGAAAGA+AGG - chr4_3:54821187-54821206 Msa0648320:three_prime_UTR 30.0%
AGAAAGAAATTAGAACCTTG+GGG + chr4_3:54821287-54821306 None:intergenic 30.0%
ACAATACTAGTTAGCTACAA+TGG - chr4_3:54821330-54821349 Msa0648320:CDS 30.0%
TATAATTCAGGAATTACCGT+TGG - chr4_3:54821421-54821440 Msa0648320:intron 30.0%
ATGAAGAGAATGTAGACATA+AGG - chr4_3:54821687-54821706 Msa0648320:CDS 30.0%
ATAAAGAGAGAGCAATTACT+AGG + chr4_3:54821838-54821857 None:intergenic 30.0%
AGATTGAAAAGGTTTGTGTA+TGG - chr4_3:54821907-54821926 Msa0648320:intron 30.0%
TTGGAACTGATCATAATATG+TGG - chr4_3:54821977-54821996 Msa0648320:intron 30.0%
TGGAACTGATCATAATATGT+GGG - chr4_3:54821978-54821997 Msa0648320:intron 30.0%
CTAAAGTTGTGATTGATTGT+CGG - chr4_3:54822124-54822143 Msa0648320:exon 30.0%
!! TTCTAAGATGAACTAGCTAG+GGG + chr4_3:54821072-54821091 None:intergenic 35.0%
TTGGTTGTATTAGAGCTAGA+AGG + chr4_3:54821177-54821196 None:intergenic 35.0%
TTTAGAGGCAAATGTTGAGA+TGG - chr4_3:54821487-54821506 Msa0648320:intron 35.0%
AGATCACGTGAATATTGCTT+AGG - chr4_3:54821551-54821570 Msa0648320:CDS 35.0%
TACAAATCATTCTCACGTGT+GGG - chr4_3:54821621-54821640 Msa0648320:CDS 35.0%
TGAATTATCTCCAACCTTAG+AGG + chr4_3:54821723-54821742 None:intergenic 35.0%
CCAAATTGAAAACAAGATGG+AGG + chr4_3:54821868-54821887 None:intergenic 35.0%
!!! CCTCCATCTTGTTTTCAATT+TGG - chr4_3:54821865-54821884 Msa0648320:intron 35.0%
GCTCCAAATTGAAAACAAGA+TGG + chr4_3:54821871-54821890 None:intergenic 35.0%
AAGTGAGTGAGAGATTGAAA+AGG - chr4_3:54821896-54821915 Msa0648320:intron 35.0%
ATTTCAAAATCCCACAACTC+AGG + chr4_3:54821958-54821977 None:intergenic 35.0%
!!! GAGTTGTGGGATTTTGAAAT+TGG - chr4_3:54821958-54821977 Msa0648320:intron 35.0%
GGAACTGATCATAATATGTG+GGG - chr4_3:54821979-54821998 Msa0648320:intron 35.0%
!! AATATGTGGGGTTTCTAGTT+AGG - chr4_3:54821991-54822010 Msa0648320:CDS 35.0%
!! TTCTAAGATGAACTAGCTAG+GGG + chr4_3:54821072-54821091 None:intergenic 35.0%
TTGGTTGTATTAGAGCTAGA+AGG + chr4_3:54821177-54821196 None:intergenic 35.0%
TTTAGAGGCAAATGTTGAGA+TGG - chr4_3:54821487-54821506 Msa0648320:intron 35.0%
AGATCACGTGAATATTGCTT+AGG - chr4_3:54821551-54821570 Msa0648320:CDS 35.0%
TACAAATCATTCTCACGTGT+GGG - chr4_3:54821621-54821640 Msa0648320:CDS 35.0%
TGAATTATCTCCAACCTTAG+AGG + chr4_3:54821723-54821742 None:intergenic 35.0%
CCAAATTGAAAACAAGATGG+AGG + chr4_3:54821868-54821887 None:intergenic 35.0%
!!! CCTCCATCTTGTTTTCAATT+TGG - chr4_3:54821865-54821884 Msa0648320:intron 35.0%
GCTCCAAATTGAAAACAAGA+TGG + chr4_3:54821871-54821890 None:intergenic 35.0%
AAGTGAGTGAGAGATTGAAA+AGG - chr4_3:54821896-54821915 Msa0648320:intron 35.0%
ATTTCAAAATCCCACAACTC+AGG + chr4_3:54821958-54821977 None:intergenic 35.0%
!!! GAGTTGTGGGATTTTGAAAT+TGG - chr4_3:54821958-54821977 Msa0648320:intron 35.0%
GGAACTGATCATAATATGTG+GGG - chr4_3:54821979-54821998 Msa0648320:intron 35.0%
!! AATATGTGGGGTTTCTAGTT+AGG - chr4_3:54821991-54822010 Msa0648320:CDS 35.0%
AACCAAAGTTGAAAGAAGGC+TGG - chr4_3:54821191-54821210 Msa0648320:three_prime_UTR 40.0%
AACCAGCCTTCTTTCAACTT+TGG + chr4_3:54821196-54821215 None:intergenic 40.0%
GAACCTTGGGGTTATAGTTA+GGG + chr4_3:54821275-54821294 None:intergenic 40.0%
AGAACCTTGGGGTTATAGTT+AGG + chr4_3:54821276-54821295 None:intergenic 40.0%
TGGCTAAGGAGAAGAAAGAA+AGG - chr4_3:54821507-54821526 Msa0648320:intron 40.0%
CTACAAATCATTCTCACGTG+TGG - chr4_3:54821620-54821639 Msa0648320:CDS 40.0%
!! AAGGAGTGTCAATTCCTCTA+AGG - chr4_3:54821706-54821725 Msa0648320:CDS 40.0%
!! AGTGTCAATTCCTCTAAGGT+TGG - chr4_3:54821710-54821729 Msa0648320:CDS 40.0%
AACCAAAGTTGAAAGAAGGC+TGG - chr4_3:54821191-54821210 Msa0648320:three_prime_UTR 40.0%
AACCAGCCTTCTTTCAACTT+TGG + chr4_3:54821196-54821215 None:intergenic 40.0%
GAACCTTGGGGTTATAGTTA+GGG + chr4_3:54821275-54821294 None:intergenic 40.0%
AGAACCTTGGGGTTATAGTT+AGG + chr4_3:54821276-54821295 None:intergenic 40.0%
TGGCTAAGGAGAAGAAAGAA+AGG - chr4_3:54821507-54821526 Msa0648320:intron 40.0%
CTACAAATCATTCTCACGTG+TGG - chr4_3:54821620-54821639 Msa0648320:CDS 40.0%
!! AAGGAGTGTCAATTCCTCTA+AGG - chr4_3:54821706-54821725 Msa0648320:CDS 40.0%
!! AGTGTCAATTCCTCTAAGGT+TGG - chr4_3:54821710-54821729 Msa0648320:CDS 40.0%
GGGGATATAGCTATGTAGGT+AGG + chr4_3:54821053-54821072 None:intergenic 45.0%
GCTAGGGGATATAGCTATGT+AGG + chr4_3:54821057-54821076 None:intergenic 45.0%
! GAGAGTTGAGTTTGGAGTAG+AGG + chr4_3:54821248-54821267 None:intergenic 45.0%
AGGGAGAAGAGAGTTGAGTT+TGG + chr4_3:54821256-54821275 None:intergenic 45.0%
TCTCCCTAACTATAACCCCA+AGG - chr4_3:54821269-54821288 Msa0648320:CDS 45.0%
ATCGCCTCATCTACTTGATC+CGG + chr4_3:54821452-54821471 None:intergenic 45.0%
GGCAAATGTTGAGATGGCTA+AGG - chr4_3:54821493-54821512 Msa0648320:intron 45.0%
CCTCAGCATGAACAACATGA+AGG + chr4_3:54821748-54821767 None:intergenic 45.0%
CCTTCATGTTGTTCATGCTG+AGG - chr4_3:54821745-54821764 Msa0648320:CDS 45.0%
GTATGGATCTTCCAGTGATG+TGG - chr4_3:54821924-54821943 Msa0648320:intron 45.0%
AGGCTGTATTTCCACATCAC+TGG + chr4_3:54821938-54821957 None:intergenic 45.0%
TGGAAATACAGCCTGAGTTG+TGG - chr4_3:54821944-54821963 Msa0648320:intron 45.0%
GGAAATACAGCCTGAGTTGT+GGG - chr4_3:54821945-54821964 Msa0648320:intron 45.0%
GGGGATATAGCTATGTAGGT+AGG + chr4_3:54821053-54821072 None:intergenic 45.0%
GCTAGGGGATATAGCTATGT+AGG + chr4_3:54821057-54821076 None:intergenic 45.0%
! GAGAGTTGAGTTTGGAGTAG+AGG + chr4_3:54821248-54821267 None:intergenic 45.0%
AGGGAGAAGAGAGTTGAGTT+TGG + chr4_3:54821256-54821275 None:intergenic 45.0%
TCTCCCTAACTATAACCCCA+AGG - chr4_3:54821269-54821288 Msa0648320:CDS 45.0%
ATCGCCTCATCTACTTGATC+CGG + chr4_3:54821452-54821471 None:intergenic 45.0%
GGCAAATGTTGAGATGGCTA+AGG - chr4_3:54821493-54821512 Msa0648320:intron 45.0%
CCTCAGCATGAACAACATGA+AGG + chr4_3:54821748-54821767 None:intergenic 45.0%
CCTTCATGTTGTTCATGCTG+AGG - chr4_3:54821745-54821764 Msa0648320:CDS 45.0%
GTATGGATCTTCCAGTGATG+TGG - chr4_3:54821924-54821943 Msa0648320:intron 45.0%
AGGCTGTATTTCCACATCAC+TGG + chr4_3:54821938-54821957 None:intergenic 45.0%
TGGAAATACAGCCTGAGTTG+TGG - chr4_3:54821944-54821963 Msa0648320:intron 45.0%
GGAAATACAGCCTGAGTTGT+GGG - chr4_3:54821945-54821964 Msa0648320:intron 45.0%
GCTACCGGATCAAGTAGATG+AGG - chr4_3:54821445-54821464 Msa0648320:intron 50.0%
GCTACCGGATCAAGTAGATG+AGG - chr4_3:54821445-54821464 Msa0648320:intron 50.0%
GGAATTACCGTTGGCGCTAC+CGG - chr4_3:54821430-54821449 Msa0648320:intron 55.0%
ACTTGATCCGGTAGCGCCAA+CGG + chr4_3:54821440-54821459 None:intergenic 55.0%
GGAATTACCGTTGGCGCTAC+CGG - chr4_3:54821430-54821449 Msa0648320:intron 55.0%
ACTTGATCCGGTAGCGCCAA+CGG + chr4_3:54821440-54821459 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 54821032 54822245 54821032 ID=Msa0648320;Name=Msa0648320
chr4_3 mRNA 54821032 54822245 54821032 ID=Msa0648320-mRNA-1;Parent=Msa0648320;Name=Msa0648320-mRNA-1;_AED=0.07;_eAED=0.07;_QI=118|1|1|1|1|1|3|237|207
chr4_3 exon 54821032 54821415 54821032 ID=Msa0648320-mRNA-1:exon:13925;Parent=Msa0648320-mRNA-1
chr4_3 exon 54821511 54821846 54821511 ID=Msa0648320-mRNA-1:exon:13924;Parent=Msa0648320-mRNA-1
chr4_3 exon 54821987 54822245 54821987 ID=Msa0648320-mRNA-1:exon:13923;Parent=Msa0648320-mRNA-1
chr4_3 five_prime_UTR 54822128 54822245 54822128 ID=Msa0648320-mRNA-1:five_prime_utr;Parent=Msa0648320-mRNA-1
chr4_3 CDS 54821987 54822127 54821987 ID=Msa0648320-mRNA-1:cds;Parent=Msa0648320-mRNA-1
chr4_3 CDS 54821511 54821846 54821511 ID=Msa0648320-mRNA-1:cds;Parent=Msa0648320-mRNA-1
chr4_3 CDS 54821269 54821415 54821269 ID=Msa0648320-mRNA-1:cds;Parent=Msa0648320-mRNA-1
chr4_3 three_prime_UTR 54821032 54821268 54821032 ID=Msa0648320-mRNA-1:three_prime_utr;Parent=Msa0648320-mRNA-1
Gene Sequence

>Msa0648320

ATGGATCAAATTGGAAGTGATCAACCTTCTAGCTCTAATACAACCAAAGTTGAAAGAAGGCTGGTTGAGAAAAACAGAAGAAATCAGATGAAGATCCTCTACTCCAAACTCAACTCTCTTCTCCCTAACTATAACCCCAAGGAATTACCGTTGGCGCTACCGGATCAAGTAGATGAGGCGATAAACTACATAAAAAGTTTAGAGGCAAATGTTGAGATGGCTAAGGAGAAGAAAGAAAGGTTATTAGCAGTAAAGAAGAAAAGATCACGTGAATATTGCTTAGGTCTACCAAAATCACCATGTTTTGAAATTCAAGAAATTGGTTCTTCTCTACAAATCATTCTCACGTGTGGGTTTGATAACCAATTCATTTTCTATGAAATCATTCGTGTGTTACATGAAGAGAATGTAGACATAAGGAGTGTCAATTCCTCTAAGGTTGGAGATAATTCACTCCTTCATGTTGTTCATGCTGAGATTCCTCCATCTTGTTTTCAATTTGGAGCGACGAAAGTGAGTGAGAGATTGAAAAGGTTTGTGTATGGATCTTCCAGTGATGTGGAAATACAGCCTGAGTTGTGGGATTTTGAAATTGGAACTGATCATAATATGTGGGGTTTCTAG
Protein sequence

>Msa0648320

MDQIGSDQPSSSNTTKVERRLVEKNRRNQMKILYSKLNSLLPNYNPKELPLALPDQVDEAINYIKSLEANVEMAKEKKERLLAVKKKRSREYCLGLPKSPCFEIQEIGSSLQIILTCGFDNQFIFYEIIRVLHEENVDIRSVNSSKVGDNSLLHVVHAEIPPSCFQFGATKVSERLKRFVYGSSSDVEIQPELWDFEIGTDHNMWGF*