AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0554460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0554460 MtrunA17_Chr4g0040201 94.231 208 11 1 1 207 1 208 8.64e-144 399
Msa0554460 MtrunA17_Chr2g0324511 65.409 159 45 5 8 160 9 163 5.56e-60 185
Msa0554460 MtrunA17_Chr2g0324521 59.483 116 44 2 47 160 2 116 4.87e-39 130
Msa0554460 MtrunA17_Chr2g0324571 57.500 120 42 3 47 160 2 118 4.70e-38 128
Msa0554460 MtrunA17_Chr2g0282341 44.526 137 63 6 50 180 4 133 5.29e-29 105
Msa0554460 MtrunA17_Chr2g0324531 60.465 43 17 0 159 201 2 44 1.01e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0554460 AT4G20970.1 42.328 189 89 8 8 180 2 186 2.11e-32 116
Msa0554460 AT1G10585.1 27.429 175 116 6 10 182 10 175 1.79e-11 60.8

Find 57 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTAGAAATTACTTAAAA+AGG 0.175107 4_1:-51755105 Msa0554460:five_prime_UTR
ATTTCAAAACATGGTGATTT+TGG 0.176377 4_1:+51754611 None:intergenic
CTAATATTTATATCTAATTA+TGG 0.230205 4_1:-51755057 Msa0554460:exon
TTAGGTCTATCTTAATATAT+AGG 0.260682 4_1:-51754180 Msa0554460:three_prime_UTR
AGAACCTTGGGGTTATAGTT+AGG 0.280194 4_1:+51754915 None:intergenic
TATCTAATTATGGATCAAAT+TGG 0.283616 4_1:-51755047 Msa0554460:exon
AGGGAGAAGAGAGTTGAGTT+TGG 0.296752 4_1:+51754935 None:intergenic
ATAAGAATTATGCTAGATTA+AGG 0.327945 4_1:-51753979 Msa0554460:three_prime_UTR
TAGGTCTATCTTAATATATA+GGG 0.337165 4_1:-51754179 Msa0554460:three_prime_UTR
AATATGTGGGGTTTCTAGTT+AGG 0.344816 4_1:-51754198 Msa0554460:exon
TACAAGACGATTAACTTATC+AGG 0.347401 4_1:+51753954 None:intergenic
ATATTAAGCTAAGAGATTAA+AGG 0.355914 4_1:+51754009 None:intergenic
GAGAGTTGAGTTTGGAGTAG+AGG 0.397503 4_1:+51754943 None:intergenic
TGAATTATCTCCAACCTTAG+AGG 0.408147 4_1:+51754469 None:intergenic
GGAATTACCGTTGGCGCTAC+CGG 0.409114 4_1:-51754759 Msa0554460:CDS
AACCAGCCTTCTTTCAACTT+TGG 0.410390 4_1:+51754995 None:intergenic
GAACCTTGGGGTTATAGTTA+GGG 0.410715 4_1:+51754916 None:intergenic
TGATTTCATAGAAAATGAAT+TGG 0.411346 4_1:+51754537 None:intergenic
AAGGAGTGTCAATTCCTCTA+AGG 0.421100 4_1:-51754483 Msa0554460:CDS
ATCGCCTCATCTACTTGATC+CGG 0.457881 4_1:+51754740 None:intergenic
TATGTAGGTAGGTTAAGAAT+AGG 0.460145 4_1:+51755149 None:intergenic
TGGCTAAGGAGAAGAAAGAA+AGG 0.476291 4_1:-51754682 Msa0554460:CDS
AAGTGAGTGAGAGATTGAAA+AGG 0.491808 4_1:-51754293 Msa0554460:CDS
GCTAGGGGATATAGCTATGT+AGG 0.500307 4_1:+51755134 None:intergenic
TTGGTTGTATTAGAGCTAGA+AGG 0.500610 4_1:+51755014 None:intergenic
ATGAAGAGAATGTAGACATA+AGG 0.503791 4_1:-51754502 Msa0554460:CDS
ATTTCTAAGATGAACTAGCT+AGG 0.508786 4_1:+51755117 None:intergenic
AGATTGAAAAGGTTTGTGTA+TGG 0.509660 4_1:-51754282 Msa0554460:CDS
TTTCTAAGATGAACTAGCTA+GGG 0.509844 4_1:+51755118 None:intergenic
AGATCACGTGAATATTGCTT+AGG 0.510152 4_1:-51754638 Msa0554460:CDS
TTGGAACTGATCATAATATG+TGG 0.512688 4_1:-51754212 Msa0554460:CDS
CTAAAGTTGTGATTGATTGT+CGG 0.519208 4_1:-51754065 Msa0554460:three_prime_UTR
AGGCTGTATTTCCACATCAC+TGG 0.522661 4_1:+51754254 None:intergenic
ATACAACCAAAGTTGAAAGA+AGG 0.526780 4_1:-51755001 Msa0554460:CDS
TATAATTCAGGAATTACCGT+TGG 0.530375 4_1:-51754768 Msa0554460:intron
ACTTGATCCGGTAGCGCCAA+CGG 0.534008 4_1:+51754752 None:intergenic
ATTTCTTGAATTTCAAAACA+TGG 0.541430 4_1:+51754602 None:intergenic
CCTTCATGTTGTTCATGCTG+AGG 0.541669 4_1:-51754444 Msa0554460:intron
AACCAAAGTTGAAAGAAGGC+TGG 0.543583 4_1:-51754997 Msa0554460:CDS
TTTAGAGGCAAATGTTGAGA+TGG 0.546113 4_1:-51754702 Msa0554460:CDS
TACAAATCATTCTCACGTGT+GGG 0.548323 4_1:-51754568 Msa0554460:CDS
TGGAACTGATCATAATATGT+GGG 0.553773 4_1:-51754211 Msa0554460:CDS
CTACAAATCATTCTCACGTG+TGG 0.558837 4_1:-51754569 Msa0554460:CDS
AGTGTCAATTCCTCTAAGGT+TGG 0.561526 4_1:-51754479 Msa0554460:CDS
ATTTCAAAATCCCACAACTC+AGG 0.570407 4_1:+51754234 None:intergenic
GGAAATACAGCCTGAGTTGT+GGG 0.582012 4_1:-51754244 Msa0554460:CDS
GGGGATATAGCTATGTAGGT+AGG 0.585601 4_1:+51755138 None:intergenic
GCTCCAAATTGAAAACAAGA+TGG 0.586259 4_1:+51754321 None:intergenic
TGGAAATACAGCCTGAGTTG+TGG 0.593486 4_1:-51754245 Msa0554460:CDS
GGCAAATGTTGAGATGGCTA+AGG 0.613070 4_1:-51754696 Msa0554460:CDS
CCTCAGCATGAACAACATGA+AGG 0.636646 4_1:+51754444 None:intergenic
CCAAATTGAAAACAAGATGG+AGG 0.639764 4_1:+51754324 None:intergenic
GGAACTGATCATAATATGTG+GGG 0.640483 4_1:-51754210 Msa0554460:CDS
GTATGGATCTTCCAGTGATG+TGG 0.643608 4_1:-51754265 Msa0554460:CDS
TTCTAAGATGAACTAGCTAG+GGG 0.654537 4_1:+51755119 None:intergenic
TCTCCCTAACTATAACCCCA+AGG 0.657270 4_1:-51754919 Msa0554460:intron
GCTACCGGATCAAGTAGATG+AGG 0.685176 4_1:-51754744 Msa0554460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATATTTATATCTAATTA+TGG - chr4_1:51754065-51754084 Msa0554460:three_prime_UTR 10.0%
!! CTAATATTTATATCTAATTA+TGG - chr4_1:51754065-51754084 Msa0554460:three_prime_UTR 10.0%
!! ATCTTAGAAATTACTTAAAA+AGG - chr4_1:51754017-51754036 Msa0554460:three_prime_UTR 15.0%
!! TCTTAGAAATTACTTAAAAA+GGG - chr4_1:51754018-51754037 Msa0554460:three_prime_UTR 15.0%
!! AAAAAAAAAGTTAGTTACAA+TGG - chr4_1:51754297-51754316 Msa0554460:CDS 15.0%
!!! GATGTAATTTTTTATAATTC+AGG - chr4_1:51754342-51754361 Msa0554460:intron 15.0%
!!! TTTATCTTTTACAAAACTTT+TGG + chr4_1:51754972-51754991 None:intergenic 15.0%
!!! TTTTACATTGAAGTTATATA+CGG - chr4_1:51755090-51755109 Msa0554460:five_prime_UTR 15.0%
!! ATCTTAGAAATTACTTAAAA+AGG - chr4_1:51754017-51754036 Msa0554460:three_prime_UTR 15.0%
!! TCTTAGAAATTACTTAAAAA+GGG - chr4_1:51754018-51754037 Msa0554460:three_prime_UTR 15.0%
!! AAAAAAAAAGTTAGTTACAA+TGG - chr4_1:51754297-51754316 Msa0554460:CDS 15.0%
!!! GATGTAATTTTTTATAATTC+AGG - chr4_1:51754342-51754361 Msa0554460:intron 15.0%
!!! TTTATCTTTTACAAAACTTT+TGG + chr4_1:51754972-51754991 None:intergenic 15.0%
!!! TTTTACATTGAAGTTATATA+CGG - chr4_1:51755090-51755109 Msa0554460:five_prime_UTR 15.0%
!! TATCTAATTATGGATCAAAT+TGG - chr4_1:51754075-51754094 Msa0554460:three_prime_UTR 20.0%
!! AAACTACATAAAAAGTTTAG+AGG - chr4_1:51754405-51754424 Msa0554460:intron 20.0%
!! ATTTCTTGAATTTCAAAACA+TGG + chr4_1:51754523-51754542 None:intergenic 20.0%
!!! TGTTTTGAAATTCAAGAAAT+TGG - chr4_1:51754523-51754542 Msa0554460:CDS 20.0%
!! TGATTTCATAGAAAATGAAT+TGG + chr4_1:51754588-51754607 None:intergenic 20.0%
!! TTAGGTCTATCTTAATATAT+AGG - chr4_1:51754942-51754961 Msa0554460:CDS 20.0%
!! TAGGTCTATCTTAATATATA+GGG - chr4_1:51754943-51754962 Msa0554460:CDS 20.0%
!! ATATTAAGCTAAGAGATTAA+AGG + chr4_1:51755116-51755135 None:intergenic 20.0%
!! ATAAGAATTATGCTAGATTA+AGG - chr4_1:51755143-51755162 Msa0554460:five_prime_UTR 20.0%
!! TATCTAATTATGGATCAAAT+TGG - chr4_1:51754075-51754094 Msa0554460:three_prime_UTR 20.0%
!! AAACTACATAAAAAGTTTAG+AGG - chr4_1:51754405-51754424 Msa0554460:intron 20.0%
!! ATTTCTTGAATTTCAAAACA+TGG + chr4_1:51754523-51754542 None:intergenic 20.0%
!!! TGTTTTGAAATTCAAGAAAT+TGG - chr4_1:51754523-51754542 Msa0554460:CDS 20.0%
!! TGATTTCATAGAAAATGAAT+TGG + chr4_1:51754588-51754607 None:intergenic 20.0%
!! TTAGGTCTATCTTAATATAT+AGG - chr4_1:51754942-51754961 Msa0554460:CDS 20.0%
!! TAGGTCTATCTTAATATATA+GGG - chr4_1:51754943-51754962 Msa0554460:CDS 20.0%
!! ATATTAAGCTAAGAGATTAA+AGG + chr4_1:51755116-51755135 None:intergenic 20.0%
!! ATAAGAATTATGCTAGATTA+AGG - chr4_1:51755143-51755162 Msa0554460:five_prime_UTR 20.0%
! AAGAAAGAAATTAGAACCTT+GGG + chr4_1:51754222-51754241 None:intergenic 25.0%
! AAAGAAAGAAATTAGAACCT+TGG + chr4_1:51754223-51754242 None:intergenic 25.0%
!! ATTTCAAAACATGGTGATTT+TGG + chr4_1:51754514-51754533 None:intergenic 25.0%
! AAGAAAGAAATTAGAACCTT+GGG + chr4_1:51754222-51754241 None:intergenic 25.0%
! AAAGAAAGAAATTAGAACCT+TGG + chr4_1:51754223-51754242 None:intergenic 25.0%
!! ATTTCAAAACATGGTGATTT+TGG + chr4_1:51754514-51754533 None:intergenic 25.0%
TATGTAGGTAGGTTAAGAAT+AGG + chr4_1:51753976-51753995 None:intergenic 30.0%
!! TTTCTAAGATGAACTAGCTA+GGG + chr4_1:51754007-51754026 None:intergenic 30.0%
!! ATTTCTAAGATGAACTAGCT+AGG + chr4_1:51754008-51754027 None:intergenic 30.0%
ATACAACCAAAGTTGAAAGA+AGG - chr4_1:51754121-51754140 Msa0554460:three_prime_UTR 30.0%
AGAAAGAAATTAGAACCTTG+GGG + chr4_1:51754221-51754240 None:intergenic 30.0%
ACAATACTAGTTAGCTACAA+TGG - chr4_1:51754264-51754283 Msa0554460:CDS 30.0%
TATAATTCAGGAATTACCGT+TGG - chr4_1:51754354-51754373 Msa0554460:intron 30.0%
ATGAAGAGAATGTAGACATA+AGG - chr4_1:51754620-51754639 Msa0554460:CDS 30.0%
ATAAAGAGAGAGCAATTACT+AGG + chr4_1:51754771-51754790 None:intergenic 30.0%
AGATTGAAAAGGTTTGTGTA+TGG - chr4_1:51754840-51754859 Msa0554460:intron 30.0%
TTGGAACTGATCATAATATG+TGG - chr4_1:51754910-51754929 Msa0554460:intron 30.0%
TGGAACTGATCATAATATGT+GGG - chr4_1:51754911-51754930 Msa0554460:intron 30.0%
CTAAAGTTGTGATTGATTGT+CGG - chr4_1:51755057-51755076 Msa0554460:exon 30.0%
TATGTAGGTAGGTTAAGAAT+AGG + chr4_1:51753976-51753995 None:intergenic 30.0%
!! TTTCTAAGATGAACTAGCTA+GGG + chr4_1:51754007-51754026 None:intergenic 30.0%
!! ATTTCTAAGATGAACTAGCT+AGG + chr4_1:51754008-51754027 None:intergenic 30.0%
ATACAACCAAAGTTGAAAGA+AGG - chr4_1:51754121-51754140 Msa0554460:three_prime_UTR 30.0%
AGAAAGAAATTAGAACCTTG+GGG + chr4_1:51754221-51754240 None:intergenic 30.0%
ACAATACTAGTTAGCTACAA+TGG - chr4_1:51754264-51754283 Msa0554460:CDS 30.0%
TATAATTCAGGAATTACCGT+TGG - chr4_1:51754354-51754373 Msa0554460:intron 30.0%
ATGAAGAGAATGTAGACATA+AGG - chr4_1:51754620-51754639 Msa0554460:CDS 30.0%
ATAAAGAGAGAGCAATTACT+AGG + chr4_1:51754771-51754790 None:intergenic 30.0%
AGATTGAAAAGGTTTGTGTA+TGG - chr4_1:51754840-51754859 Msa0554460:intron 30.0%
TTGGAACTGATCATAATATG+TGG - chr4_1:51754910-51754929 Msa0554460:intron 30.0%
TGGAACTGATCATAATATGT+GGG - chr4_1:51754911-51754930 Msa0554460:intron 30.0%
CTAAAGTTGTGATTGATTGT+CGG - chr4_1:51755057-51755076 Msa0554460:exon 30.0%
!! TTCTAAGATGAACTAGCTAG+GGG + chr4_1:51754006-51754025 None:intergenic 35.0%
TTGGTTGTATTAGAGCTAGA+AGG + chr4_1:51754111-51754130 None:intergenic 35.0%
TTTAGAGGCAAATGTTGAGA+TGG - chr4_1:51754420-51754439 Msa0554460:intron 35.0%
AGATCACGTGAATATTGCTT+AGG - chr4_1:51754484-51754503 Msa0554460:CDS 35.0%
TACAAATCATTCTCACGTGT+GGG - chr4_1:51754554-51754573 Msa0554460:CDS 35.0%
TGAATTATCTCCAACCTTAG+AGG + chr4_1:51754656-51754675 None:intergenic 35.0%
CCAAATTGAAAACAAGATGG+AGG + chr4_1:51754801-51754820 None:intergenic 35.0%
!!! CCTCCATCTTGTTTTCAATT+TGG - chr4_1:51754798-51754817 Msa0554460:intron 35.0%
GCTCCAAATTGAAAACAAGA+TGG + chr4_1:51754804-51754823 None:intergenic 35.0%
AAGTGAGTGAGAGATTGAAA+AGG - chr4_1:51754829-51754848 Msa0554460:intron 35.0%
ATTTCAAAATCCCACAACTC+AGG + chr4_1:51754891-51754910 None:intergenic 35.0%
!!! GAGTTGTGGGATTTTGAAAT+TGG - chr4_1:51754891-51754910 Msa0554460:intron 35.0%
GGAACTGATCATAATATGTG+GGG - chr4_1:51754912-51754931 Msa0554460:intron 35.0%
!! AATATGTGGGGTTTCTAGTT+AGG - chr4_1:51754924-51754943 Msa0554460:CDS 35.0%
!! TTCTAAGATGAACTAGCTAG+GGG + chr4_1:51754006-51754025 None:intergenic 35.0%
TTGGTTGTATTAGAGCTAGA+AGG + chr4_1:51754111-51754130 None:intergenic 35.0%
TTTAGAGGCAAATGTTGAGA+TGG - chr4_1:51754420-51754439 Msa0554460:intron 35.0%
AGATCACGTGAATATTGCTT+AGG - chr4_1:51754484-51754503 Msa0554460:CDS 35.0%
TACAAATCATTCTCACGTGT+GGG - chr4_1:51754554-51754573 Msa0554460:CDS 35.0%
TGAATTATCTCCAACCTTAG+AGG + chr4_1:51754656-51754675 None:intergenic 35.0%
CCAAATTGAAAACAAGATGG+AGG + chr4_1:51754801-51754820 None:intergenic 35.0%
!!! CCTCCATCTTGTTTTCAATT+TGG - chr4_1:51754798-51754817 Msa0554460:intron 35.0%
GCTCCAAATTGAAAACAAGA+TGG + chr4_1:51754804-51754823 None:intergenic 35.0%
AAGTGAGTGAGAGATTGAAA+AGG - chr4_1:51754829-51754848 Msa0554460:intron 35.0%
ATTTCAAAATCCCACAACTC+AGG + chr4_1:51754891-51754910 None:intergenic 35.0%
!!! GAGTTGTGGGATTTTGAAAT+TGG - chr4_1:51754891-51754910 Msa0554460:intron 35.0%
GGAACTGATCATAATATGTG+GGG - chr4_1:51754912-51754931 Msa0554460:intron 35.0%
!! AATATGTGGGGTTTCTAGTT+AGG - chr4_1:51754924-51754943 Msa0554460:CDS 35.0%
AACCAAAGTTGAAAGAAGGC+TGG - chr4_1:51754125-51754144 Msa0554460:three_prime_UTR 40.0%
AACCAGCCTTCTTTCAACTT+TGG + chr4_1:51754130-51754149 None:intergenic 40.0%
GAACCTTGGGGTTATAGTTA+GGG + chr4_1:51754209-51754228 None:intergenic 40.0%
AGAACCTTGGGGTTATAGTT+AGG + chr4_1:51754210-51754229 None:intergenic 40.0%
TGGCTAAGGAGAAGAAAGAA+AGG - chr4_1:51754440-51754459 Msa0554460:intron 40.0%
CTACAAATCATTCTCACGTG+TGG - chr4_1:51754553-51754572 Msa0554460:CDS 40.0%
!! AAGGAGTGTCAATTCCTCTA+AGG - chr4_1:51754639-51754658 Msa0554460:CDS 40.0%
!! AGTGTCAATTCCTCTAAGGT+TGG - chr4_1:51754643-51754662 Msa0554460:CDS 40.0%
AACCAAAGTTGAAAGAAGGC+TGG - chr4_1:51754125-51754144 Msa0554460:three_prime_UTR 40.0%
AACCAGCCTTCTTTCAACTT+TGG + chr4_1:51754130-51754149 None:intergenic 40.0%
GAACCTTGGGGTTATAGTTA+GGG + chr4_1:51754209-51754228 None:intergenic 40.0%
AGAACCTTGGGGTTATAGTT+AGG + chr4_1:51754210-51754229 None:intergenic 40.0%
TGGCTAAGGAGAAGAAAGAA+AGG - chr4_1:51754440-51754459 Msa0554460:intron 40.0%
CTACAAATCATTCTCACGTG+TGG - chr4_1:51754553-51754572 Msa0554460:CDS 40.0%
!! AAGGAGTGTCAATTCCTCTA+AGG - chr4_1:51754639-51754658 Msa0554460:CDS 40.0%
!! AGTGTCAATTCCTCTAAGGT+TGG - chr4_1:51754643-51754662 Msa0554460:CDS 40.0%
GGGGATATAGCTATGTAGGT+AGG + chr4_1:51753987-51754006 None:intergenic 45.0%
GCTAGGGGATATAGCTATGT+AGG + chr4_1:51753991-51754010 None:intergenic 45.0%
! GAGAGTTGAGTTTGGAGTAG+AGG + chr4_1:51754182-51754201 None:intergenic 45.0%
AGGGAGAAGAGAGTTGAGTT+TGG + chr4_1:51754190-51754209 None:intergenic 45.0%
TCTCCCTAACTATAACCCCA+AGG - chr4_1:51754203-51754222 Msa0554460:CDS 45.0%
ATCGCCTCATCTACTTGATC+CGG + chr4_1:51754385-51754404 None:intergenic 45.0%
GGCAAATGTTGAGATGGCTA+AGG - chr4_1:51754426-51754445 Msa0554460:intron 45.0%
CCTCAGCATGAACAACATGA+AGG + chr4_1:51754681-51754700 None:intergenic 45.0%
CCTTCATGTTGTTCATGCTG+AGG - chr4_1:51754678-51754697 Msa0554460:CDS 45.0%
GTATGGATCTTCCAGTGATG+TGG - chr4_1:51754857-51754876 Msa0554460:intron 45.0%
AGGCTGTATTTCCACATCAC+TGG + chr4_1:51754871-51754890 None:intergenic 45.0%
TGGAAATACAGCCTGAGTTG+TGG - chr4_1:51754877-51754896 Msa0554460:intron 45.0%
GGAAATACAGCCTGAGTTGT+GGG - chr4_1:51754878-51754897 Msa0554460:intron 45.0%
GGGGATATAGCTATGTAGGT+AGG + chr4_1:51753987-51754006 None:intergenic 45.0%
GCTAGGGGATATAGCTATGT+AGG + chr4_1:51753991-51754010 None:intergenic 45.0%
! GAGAGTTGAGTTTGGAGTAG+AGG + chr4_1:51754182-51754201 None:intergenic 45.0%
AGGGAGAAGAGAGTTGAGTT+TGG + chr4_1:51754190-51754209 None:intergenic 45.0%
TCTCCCTAACTATAACCCCA+AGG - chr4_1:51754203-51754222 Msa0554460:CDS 45.0%
ATCGCCTCATCTACTTGATC+CGG + chr4_1:51754385-51754404 None:intergenic 45.0%
GGCAAATGTTGAGATGGCTA+AGG - chr4_1:51754426-51754445 Msa0554460:intron 45.0%
CCTCAGCATGAACAACATGA+AGG + chr4_1:51754681-51754700 None:intergenic 45.0%
CCTTCATGTTGTTCATGCTG+AGG - chr4_1:51754678-51754697 Msa0554460:CDS 45.0%
GTATGGATCTTCCAGTGATG+TGG - chr4_1:51754857-51754876 Msa0554460:intron 45.0%
AGGCTGTATTTCCACATCAC+TGG + chr4_1:51754871-51754890 None:intergenic 45.0%
TGGAAATACAGCCTGAGTTG+TGG - chr4_1:51754877-51754896 Msa0554460:intron 45.0%
GGAAATACAGCCTGAGTTGT+GGG - chr4_1:51754878-51754897 Msa0554460:intron 45.0%
GCTACCGGATCAAGTAGATG+AGG - chr4_1:51754378-51754397 Msa0554460:intron 50.0%
GCTACCGGATCAAGTAGATG+AGG - chr4_1:51754378-51754397 Msa0554460:intron 50.0%
GGAATTACCGTTGGCGCTAC+CGG - chr4_1:51754363-51754382 Msa0554460:intron 55.0%
ACTTGATCCGGTAGCGCCAA+CGG + chr4_1:51754373-51754392 None:intergenic 55.0%
GGAATTACCGTTGGCGCTAC+CGG - chr4_1:51754363-51754382 Msa0554460:intron 55.0%
ACTTGATCCGGTAGCGCCAA+CGG + chr4_1:51754373-51754392 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 51753966 51755178 51753966 ID=Msa0554460;Name=Msa0554460
chr4_1 mRNA 51753966 51755178 51753966 ID=Msa0554460-mRNA-1;Parent=Msa0554460;Name=Msa0554460-mRNA-1;_AED=0.08;_eAED=0.08;_QI=118|1|1|1|1|1|3|237|207
chr4_1 exon 51753966 51754349 51753966 ID=Msa0554460-mRNA-1:exon:14888;Parent=Msa0554460-mRNA-1
chr4_1 exon 51754445 51754780 51754445 ID=Msa0554460-mRNA-1:exon:14887;Parent=Msa0554460-mRNA-1
chr4_1 exon 51754920 51755178 51754920 ID=Msa0554460-mRNA-1:exon:14886;Parent=Msa0554460-mRNA-1
chr4_1 five_prime_UTR 51755061 51755178 51755061 ID=Msa0554460-mRNA-1:five_prime_utr;Parent=Msa0554460-mRNA-1
chr4_1 CDS 51754920 51755060 51754920 ID=Msa0554460-mRNA-1:cds;Parent=Msa0554460-mRNA-1
chr4_1 CDS 51754445 51754780 51754445 ID=Msa0554460-mRNA-1:cds;Parent=Msa0554460-mRNA-1
chr4_1 CDS 51754203 51754349 51754203 ID=Msa0554460-mRNA-1:cds;Parent=Msa0554460-mRNA-1
chr4_1 three_prime_UTR 51753966 51754202 51753966 ID=Msa0554460-mRNA-1:three_prime_utr;Parent=Msa0554460-mRNA-1
Gene Sequence

>Msa0554460

ATGGATCAAATTGGAAGTGATCAACCTTCTAGCTCTAATACAACCAAAGTTGAAAGAAGGCTGGTTGAGAAAAACAGAAGAAATCAGATGAAGATCCTCTACTCCAAACTCAACTCTCTTCTCCCTAACTATAACCCCAAGGAATTACCGTTGGCGCTACCGGATCAAGTAGATGAGGCGATAAACTACATAAAAAGTTTAGAGGCAAATGTTGAGATGGCTAAGGAGAAGAAAGAAAGGTTATTAGCAGTAAAGAAGAAAAGATCACGTGAATATTGCTTAGGTCTACCAAAATCACCATGTTTTGAAATTCAAGAAATTGGTTCTTCTCTACAAATCATTCTCACGTGTGGGTTTGATAACCAATTCATTTTCTATGAAATCATTCGTGTGTTACATGAAGAGAATGTAGACATAAGGAGTGTCAATTCCTCTAAGGTTGGAGATAATTCACTCCTTCATGTTGTTCATGCTGAGATTCCTCCATCTTGTTTTCAATTTGGAGCGACGAAAGTGAGTGAGAGATTGAAAAGGTTTGTGTATGGATCTTCCAGTGATGTGGAAATACAGCCTGAGTTGTGGGATTTTGAAATTGGAACTGATCATAATATGTGGGGTTTCTAG
Protein sequence

>Msa0554460

MDQIGSDQPSSSNTTKVERRLVEKNRRNQMKILYSKLNSLLPNYNPKELPLALPDQVDEAINYIKSLEANVEMAKEKKERLLAVKKKRSREYCLGLPKSPCFEIQEIGSSLQIILTCGFDNQFIFYEIIRVLHEENVDIRSVNSSKVGDNSLLHVVHAEIPPSCFQFGATKVSERLKRFVYGSSSDVEIQPELWDFEIGTDHNMWGF*