AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0653030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0653030 MtrunA17_Chr4g0047001 93.316 389 9 6 1 377 1 384 0.0 721
Msa0653030 MtrunA17_Chr2g0282511 41.842 380 135 17 8 377 6 309 6.83e-49 167
Msa0653030 MtrunA17_Chr8g0367161 62.651 83 31 0 45 127 23 105 3.84e-34 129
Msa0653030 MtrunA17_Chr8g0374251 65.432 81 28 0 49 129 63 143 6.88e-33 125
Msa0653030 MtrunA17_Chr2g0328021 76.562 64 15 0 54 117 62 125 1.09e-32 124
Msa0653030 MtrunA17_Chr7g0237921 60.870 92 30 1 17 108 45 130 1.53e-32 125
Msa0653030 MtrunA17_Chr4g0031411 62.222 90 30 1 47 132 68 157 1.84e-32 124
Msa0653030 MtrunA17_Chr2g0326001 83.333 60 10 0 49 108 35 94 3.12e-32 122
Msa0653030 MtrunA17_Chr8g0346551 53.333 105 39 1 43 147 13 107 3.29e-32 122
Msa0653030 MtrunA17_Chr4g0010151 46.032 126 68 0 47 172 75 200 3.43e-32 124
Msa0653030 MtrunA17_Chr4g0030051 85.965 57 8 0 50 106 37 93 1.11e-31 121
Msa0653030 MtrunA17_Chr3g0127201 71.233 73 16 1 41 108 9 81 1.37e-31 121
Msa0653030 MtrunA17_Chr8g0374111 65.789 76 26 0 50 125 25 100 2.57e-31 118
Msa0653030 MtrunA17_Chr3g0126201 55.882 102 38 2 15 110 7 107 5.42e-31 120
Msa0653030 MtrunA17_Chr2g0282441 77.049 61 14 0 45 105 16 76 4.15e-30 118
Msa0653030 MtrunA17_Chr2g0282051 66.667 78 19 2 39 110 25 101 4.19e-30 116
Msa0653030 MtrunA17_Chr5g0411951 79.630 54 11 0 51 104 40 93 2.82e-29 115
Msa0653030 MtrunA17_Chr4g0047761 72.973 74 19 1 38 111 56 128 3.87e-29 115
Msa0653030 MtrunA17_Chr1g0177251 79.310 58 12 0 51 108 9 66 4.40e-29 114
Msa0653030 MtrunA17_Chr1g0188851 67.606 71 21 1 40 108 27 97 5.35e-29 114
Msa0653030 MtrunA17_Chr8g0341671 78.571 56 12 0 50 105 16 71 8.95e-29 111
Msa0653030 MtrunA17_Chr4g0061761 81.132 53 10 0 52 104 38 90 2.47e-28 112
Msa0653030 MtrunA17_Chr3g0117601 72.131 61 17 0 44 104 51 111 2.73e-28 113
Msa0653030 MtrunA17_Chr2g0308011 84.000 50 8 0 53 102 36 85 9.47e-27 105
Msa0653030 MtrunA17_Chr3g0092111 65.625 64 22 0 44 107 126 189 2.25e-26 109
Msa0653030 MtrunA17_Chr4g0042481 62.121 66 25 0 44 109 112 177 7.61e-26 108
Msa0653030 MtrunA17_Chr7g0254161 63.077 65 24 0 45 109 92 156 1.32e-25 107
Msa0653030 MtrunA17_Chr7g0217581 65.079 63 22 0 45 107 223 285 4.52e-25 106
Msa0653030 MtrunA17_Chr6g0453321 67.213 61 20 0 47 107 129 189 7.74e-25 105
Msa0653030 MtrunA17_Chr8g0355261 45.918 98 43 2 13 108 83 172 1.20e-24 104
Msa0653030 MtrunA17_Chr2g0283811 50.000 94 38 2 44 137 37 121 1.97e-24 98.2
Msa0653030 MtrunA17_Chr6g0460721 56.923 65 28 0 45 109 80 144 2.59e-24 102
Msa0653030 MtrunA17_Chr6g0460731 58.462 65 27 0 45 109 87 151 5.35e-24 101
Msa0653030 MtrunA17_Chr5g0417571 63.333 60 22 0 48 107 132 191 4.95e-23 98.2
Msa0653030 MtrunA17_Chr5g0417601 50.000 88 36 1 20 107 114 193 2.27e-21 94.4
Msa0653030 MtrunA17_Chr5g0417551 67.857 56 18 0 52 107 125 180 4.97e-21 93.6
Msa0653030 MtrunA17_Chr5g0417561 70.370 54 16 0 54 107 119 172 6.47e-21 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0653030 AT5G65590.1 38.645 251 111 9 24 269 17 229 8.70e-37 135
Msa0653030 AT1G28310.1 37.798 336 146 17 54 377 27 311 2.29e-35 132
Msa0653030 AT1G28310.2 35.969 392 179 19 1 377 1 335 2.37e-35 132
Msa0653030 AT5G60200.1 81.967 61 11 0 48 108 49 109 7.55e-34 126
Msa0653030 AT3G61850.2 41.818 165 77 4 49 194 57 221 1.12e-32 124
Msa0653030 AT3G61850.3 41.818 165 77 4 49 194 57 221 1.12e-32 124
Msa0653030 AT3G61850.1 42.331 163 75 4 51 194 71 233 1.17e-32 124
Msa0653030 AT2G37590.1 65.753 73 25 0 38 110 73 145 2.91e-32 124
Msa0653030 AT5G02460.1 82.759 58 10 0 51 108 92 149 2.50e-31 123
Msa0653030 AT3G55370.1 60.000 85 34 0 26 110 48 132 2.73e-31 121
Msa0653030 AT3G55370.2 60.000 85 34 0 26 110 48 132 4.33e-31 121
Msa0653030 AT3G55370.3 60.000 85 34 0 26 110 93 177 4.73e-31 121
Msa0653030 AT1G21340.1 64.557 79 25 1 39 114 20 98 5.24e-31 119
Msa0653030 AT2G28810.2 79.310 58 12 0 48 105 71 128 5.69e-31 120
Msa0653030 AT5G60850.1 70.667 75 19 1 52 123 49 123 6.24e-31 120
Msa0653030 AT1G07640.1 46.032 126 64 1 35 160 2 123 6.56e-31 119
Msa0653030 AT3G50410.1 67.532 77 23 2 37 111 11 87 6.94e-31 118
Msa0653030 AT2G28810.1 79.310 58 12 0 48 105 88 145 7.67e-31 120
Msa0653030 AT1G07640.2 48.673 113 51 2 3 108 19 131 8.81e-31 120
Msa0653030 AT1G07640.3 48.673 113 51 2 3 108 27 139 9.05e-31 120
Msa0653030 AT3G45610.1 77.586 58 13 0 51 108 37 94 1.28e-30 117
Msa0653030 AT2G28510.1 82.456 57 10 0 52 108 46 102 1.55e-30 118
Msa0653030 AT1G51700.1 60.215 93 28 3 17 108 4 88 2.45e-30 115
Msa0653030 AT3G61850.5 53.271 107 41 2 49 146 57 163 2.77e-30 118
Msa0653030 AT3G61850.4 53.271 107 41 2 49 146 69 175 3.52e-30 118
Msa0653030 AT3G52440.1 81.818 55 10 0 56 110 27 81 2.76e-29 114
Msa0653030 AT3G52440.2 81.818 55 10 0 56 110 46 100 4.31e-29 114
Msa0653030 AT3G21270.1 84.906 53 8 0 54 106 29 81 6.59e-29 112
Msa0653030 AT4G00940.1 74.603 63 16 0 51 113 65 127 6.87e-29 114
Msa0653030 AT4G00940.3 74.603 63 16 0 51 113 65 127 6.87e-29 114
Msa0653030 AT4G00940.2 74.603 63 16 0 51 113 65 127 6.87e-29 114
Msa0653030 AT4G24060.1 75.000 60 15 0 49 108 48 107 9.39e-29 115
Msa0653030 AT4G21050.1 60.000 80 32 0 47 126 17 96 2.70e-28 110
Msa0653030 AT5G66940.1 56.977 86 33 2 50 132 28 112 2.80e-28 110
Msa0653030 AT4G38000.1 79.310 58 11 1 51 107 38 95 8.81e-28 110
Msa0653030 AT5G62940.1 69.355 62 19 0 43 104 62 123 4.53e-27 110
Msa0653030 AT3G47500.1 66.667 66 22 0 42 107 98 163 3.28e-26 109
Msa0653030 AT5G62430.1 44.860 107 49 2 1 107 11 107 1.38e-25 105
Msa0653030 AT1G64620.1 74.545 55 14 0 49 103 44 98 3.28e-25 105
Msa0653030 AT2G46590.1 78.431 51 11 0 51 101 65 115 4.01e-25 105
Msa0653030 AT2G46590.2 78.431 51 11 0 51 101 77 127 4.72e-25 105
Msa0653030 AT5G39660.3 68.852 61 19 0 47 107 131 191 5.03e-25 106
Msa0653030 AT5G39660.1 68.852 61 19 0 47 107 131 191 5.03e-25 106
Msa0653030 AT5G39660.2 68.852 61 19 0 47 107 131 191 5.03e-25 106
Msa0653030 AT1G26790.1 63.934 61 22 0 47 107 104 164 2.66e-24 103
Msa0653030 AT1G69570.1 60.294 68 27 0 47 114 125 192 2.69e-24 103
Msa0653030 AT1G47655.1 72.549 51 14 0 52 102 27 77 9.28e-24 98.2
Msa0653030 AT2G34140.1 54.321 81 30 1 27 107 38 111 1.42e-23 96.7
Msa0653030 AT1G29160.1 48.315 89 44 1 39 125 45 133 2.03e-23 96.3
Msa0653030 AT4G21040.1 57.143 77 30 2 56 131 27 101 3.20e-23 97.4
Msa0653030 AT4G21080.1 65.517 58 19 1 56 112 27 84 3.52e-21 92.0
Msa0653030 AT4G21030.1 46.053 76 41 0 56 131 23 98 3.75e-14 70.9

Find 129 sgRNAs with CRISPR-Local

Find 328 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATCAATTCTCTTCTTTA+TGG 0.060643 4_3:+60857899 Msa0653030:CDS
CAGATTCTTCTTCTCTTTAT+TGG 0.115189 4_3:+60858431 Msa0653030:CDS
AACAGCAACAACAAGCTTTA+AGG 0.128070 4_3:+60857576 Msa0653030:CDS
TCAAAACCAGATGGAGAATA+TGG 0.176496 4_3:+60858402 Msa0653030:CDS
ATGTTTCAAGGTTGAGAATT+TGG 0.186147 4_3:+60858795 Msa0653030:three_prime_UTR
CTTCTTCAAGTGGTGGAAAT+AGG 0.186986 4_3:+60857522 Msa0653030:CDS
TAATATTATTTACTTTGATT+TGG 0.224707 4_3:+60859181 Msa0653030:three_prime_UTR
GGGTTAGGTTATGGGGTTAT+TGG 0.224858 4_3:+60859126 Msa0653030:three_prime_UTR
GAATTATGACCAATATTATT+AGG 0.234994 4_3:-60858526 None:intergenic
AACACAGTTACCAAACATTT+TGG 0.243359 4_3:-60857240 None:intergenic
GCGCCTTGAGTTTCGCTTGA+TGG 0.261833 4_3:-60857170 None:intergenic
ATTTGGAAGGGGTTAGGTTA+TGG 0.265588 4_3:+60859117 Msa0653030:three_prime_UTR
TTAGTTAGGGTGGTGAGGTT+GGG 0.272906 4_3:+60859027 Msa0653030:three_prime_UTR
CAGGTGAATGGTCTTGGATT+AGG 0.278693 4_3:+60858028 Msa0653030:CDS
AATATTATTTACTTTGATTT+GGG 0.284052 4_3:+60859182 Msa0653030:three_prime_UTR
ACTAGAGTTTCAAGTTCTTC+TGG 0.293306 4_3:+60857977 Msa0653030:CDS
CATAAAGAAGAGAATTGATA+TGG 0.297144 4_3:-60857898 None:intergenic
CTTTGTTTACTCCATTTCTA+AGG 0.298192 4_3:+60857147 Msa0653030:five_prime_UTR
GCAAAGCCTGTAAAAGATAT+TGG 0.299579 4_3:+60857675 Msa0653030:CDS
AACAACTACAGTTCCATCTT+TGG 0.305663 4_3:+60858118 Msa0653030:CDS
TCATCATGAGGCTGGGATAA+TGG 0.306350 4_3:+60858291 Msa0653030:CDS
TTTGGAAGGGGTTAGGTTAT+GGG 0.308073 4_3:+60859118 Msa0653030:three_prime_UTR
TGCTGCAGTGTTGTTGTTGT+TGG 0.309230 4_3:-60858467 None:intergenic
ATTAGTTAGGGTGGTGAGGT+TGG 0.310613 4_3:+60859026 Msa0653030:three_prime_UTR
ATGTATTATTGTGATTAGTT+AGG 0.310937 4_3:+60859013 Msa0653030:three_prime_UTR
TAGTTAGGGTGGTGAGGTTG+GGG 0.322751 4_3:+60859028 Msa0653030:three_prime_UTR
TTAGGTTATGGGGTTATTGG+AGG 0.330066 4_3:+60859129 Msa0653030:three_prime_UTR
CATTTGGGGTCAATTGGGTT+TGG 0.331429 4_3:+60858659 Msa0653030:three_prime_UTR
TGTTTCAAGGTTGAGAATTT+GGG 0.332884 4_3:+60858796 Msa0653030:three_prime_UTR
GCTGCTGCTGCTGGTGGTGG+TGG 0.343979 4_3:+60858247 Msa0653030:CDS
GATGATTACAGAAATGGATT+TGG 0.345610 4_3:+60858085 Msa0653030:CDS
CTTACTCAATCAATCAACTA+TGG 0.352717 4_3:-60858920 None:intergenic
ATCTAGCTATCTCTTCTATT+AGG 0.360530 4_3:+60858597 Msa0653030:three_prime_UTR
CATCATGATGATTACAGAAA+TGG 0.360895 4_3:+60858079 Msa0653030:CDS
CAACAGGTTTGAGTAGTGTT+TGG 0.362182 4_3:+60858488 Msa0653030:CDS
ACTTAAAGGTGAGAGAGTTT+AGG 0.366369 4_3:+60858718 Msa0653030:three_prime_UTR
CTTAACATTTGGAAGGGGTT+AGG 0.368481 4_3:+60859111 Msa0653030:three_prime_UTR
TTCTTCAAGTGGTGGAAATA+GGG 0.372187 4_3:+60857523 Msa0653030:CDS
GGATATGGAGGTTCTTCATT+TGG 0.372923 4_3:+60858886 Msa0653030:three_prime_UTR
TTGTTAACTTTCTTAACATT+TGG 0.385994 4_3:+60859100 Msa0653030:three_prime_UTR
TTAAGTAGAGAAGCCATAAC+TGG 0.397152 4_3:-60858187 None:intergenic
TGTATTATTGTGATTAGTTA+GGG 0.397649 4_3:+60859014 Msa0653030:three_prime_UTR
GGAGCTCAGATCAAGGGATA+TGG 0.400830 4_3:+60858871 Msa0653030:three_prime_UTR
CAGCCTCAGGTGAATGGTCT+TGG 0.403606 4_3:+60858022 Msa0653030:CDS
TCGCTTGATGGCCTTAGAAA+TGG 0.404180 4_3:-60857158 None:intergenic
TATGTATGTGGGATTGGTTT+TGG 0.404272 4_3:+60859212 Msa0653030:three_prime_UTR
AGTACAGCTGCTGCTGCTGC+TGG 0.409160 4_3:+60858238 Msa0653030:CDS
AAGAGAATTGATATGGTTTG+AGG 0.411504 4_3:-60857891 None:intergenic
GATCACCAACCTAATAATAT+TGG 0.414706 4_3:+60858517 Msa0653030:CDS
CTCATAGAGACCAAAATGTT+TGG 0.415114 4_3:+60857230 Msa0653030:exon
TAGAAGCAATATCAAAACTA+TGG 0.419426 4_3:-60857868 None:intergenic
ATGTTTGAAGAAGAGTGATT+TGG 0.420876 4_3:-60857767 None:intergenic
GATTAATATGTATGTGGGAT+TGG 0.432502 4_3:+60859206 Msa0653030:three_prime_UTR
CAAAACCAGATGGAGAATAT+GGG 0.434787 4_3:+60858403 Msa0653030:CDS
AACAACAACACTGCAGCAAC+AGG 0.445401 4_3:+60858472 Msa0653030:CDS
AAGTGGTGGAAATAGGGTTA+TGG 0.446908 4_3:+60857529 Msa0653030:CDS
TAGGTGAAGGACTACAAAAT+AGG 0.461358 4_3:+60858332 Msa0653030:CDS
TATAATTGTGGTATAGAAGA+GGG 0.462670 4_3:+60859152 Msa0653030:three_prime_UTR
GGCCTCTACTACTCCAGTTA+TGG 0.463925 4_3:+60858174 Msa0653030:CDS
GGGTTTGAATTAGCACTAGT+AGG 0.464115 4_3:-60857824 None:intergenic
GAATTGAACCTTGTTGCAAA+AGG 0.464424 4_3:-60857953 None:intergenic
ACAAACCCATATTCTCCATC+TGG 0.464554 4_3:-60858408 None:intergenic
TGGGTTTGGTTTACTTACAT+TGG 0.466899 4_3:+60858673 Msa0653030:three_prime_UTR
TATGACCAATATTATTAGGT+TGG 0.469646 4_3:-60858522 None:intergenic
TTTCTGCAACCACCACCAAC+AGG 0.472277 4_3:-60857725 None:intergenic
GCTGGTGGTGGTGGAATGAA+AGG 0.473306 4_3:+60858256 Msa0653030:CDS
TCTTATCATGCTAGCTAGCT+AGG 0.474218 4_3:+60857109 Msa0653030:five_prime_UTR
CAGGCTTTGCAGAAGTGTCT+AGG 0.477081 4_3:-60857662 None:intergenic
ATCCCAGCCTCATGATGAAA+AGG 0.481394 4_3:-60858286 None:intergenic
AACTTTCTTAACATTTGGAA+GGG 0.489571 4_3:+60859105 Msa0653030:three_prime_UTR
TGTCTGTGGAGCTCAGATCA+AGG 0.492001 4_3:+60858864 Msa0653030:three_prime_UTR
AGTTGCATGCGGATGATAAT+CGG 0.498977 4_3:+60857053 Msa0653030:five_prime_UTR
CCTTGAAACATAAAACTGAC+AGG 0.508654 4_3:-60858783 None:intergenic
TTGGGTGATTAATATGTATG+TGG 0.510167 4_3:+60859200 Msa0653030:three_prime_UTR
GTTGTTGTTGTTGAGAAGGA+GGG 0.513508 4_3:-60857844 None:intergenic
AGATGATCATAAGATTTCAA+TGG 0.515408 4_3:+60857499 Msa0653030:CDS
CAGCAGCAGCCTCAGGTGAA+TGG 0.518167 4_3:+60858016 Msa0653030:CDS
CTTAGAAACGTTCCTGTTGG+TGG 0.520182 4_3:+60857713 Msa0653030:CDS
AGTTAGGGTGGTGAGGTTGG+GGG 0.520440 4_3:+60859029 Msa0653030:three_prime_UTR
AGTAATAATATGAATGCTAA+TGG 0.525718 4_3:+60858367 Msa0653030:CDS
AACTGGAGTAGTAGAGGCCA+TGG 0.527816 4_3:-60858170 None:intergenic
AGTTGATTGATTGAGTAAGT+TGG 0.528561 4_3:+60858924 Msa0653030:three_prime_UTR
TGTGATTAGTTAGGGTGGTG+AGG 0.530440 4_3:+60859022 Msa0653030:three_prime_UTR
TGTTGTTGTTGTTGAGAAGG+AGG 0.530854 4_3:-60857845 None:intergenic
TAACTTTCTTAACATTTGGA+AGG 0.532329 4_3:+60859104 Msa0653030:three_prime_UTR
TGAAGGACTACAAAATAGGT+TGG 0.535498 4_3:+60858336 Msa0653030:CDS
GATATCAGACAAAGAGCAGT+TGG 0.536328 4_3:-60857192 None:intergenic
AGTAGAAGAAGAACCAAAGA+TGG 0.540629 4_3:-60858131 None:intergenic
TGGTGTTGTTGTTGTTGAGA+AGG 0.544856 4_3:-60857848 None:intergenic
CTAGTAGGAGTTGAAGAAGA+AGG 0.548358 4_3:-60857809 None:intergenic
TCTTCAGCAGCAGCAGCCTC+AGG 0.551095 4_3:+60858009 Msa0653030:CDS
ATAATTGTGGTATAGAAGAG+GGG 0.552388 4_3:+60859153 Msa0653030:three_prime_UTR
TGTAAAAGATATTGGACAAG+AGG 0.561250 4_3:+60857683 Msa0653030:CDS
ATTTCAATGGCTTCTTCAAG+TGG 0.564116 4_3:+60857512 Msa0653030:CDS
TCAATGGCTTCTTCAAGTGG+TGG 0.566820 4_3:+60857515 Msa0653030:CDS
TGGGTGATTAATATGTATGT+GGG 0.569054 4_3:+60859201 Msa0653030:three_prime_UTR
AGCCATAACTGGAGTAGTAG+AGG 0.573379 4_3:-60858176 None:intergenic
TTTACTCACTATATGATGAA+AGG 0.576294 4_3:+60859053 Msa0653030:three_prime_UTR
AGAAACGTTCCTGTTGGTGG+TGG 0.578028 4_3:+60857716 Msa0653030:CDS
TGTTGAAGAAGCTCTTGACC+TGG 0.579160 4_3:-60857557 None:intergenic
TTGGAAGGGGTTAGGTTATG+GGG 0.580314 4_3:+60859119 Msa0653030:three_prime_UTR
ACTTTCTTAACATTTGGAAG+GGG 0.581154 4_3:+60859106 Msa0653030:three_prime_UTR
TACAAAATAGGTTGGATCAG+TGG 0.582074 4_3:+60858344 Msa0653030:CDS
AAAAGATATTGGACAAGAGG+TGG 0.582761 4_3:+60857686 Msa0653030:CDS
GGTGGTGGTGGAATGAAAGG+TGG 0.591912 4_3:+60858259 Msa0653030:CDS
GTCTGTGGAGCTCAGATCAA+GGG 0.592725 4_3:+60858865 Msa0653030:three_prime_UTR
GGTTATTGGAGGTATAATTG+TGG 0.592790 4_3:+60859140 Msa0653030:three_prime_UTR
GTATAATTGTGGTATAGAAG+AGG 0.594629 4_3:+60859151 Msa0653030:three_prime_UTR
TAACTGTGTTGATGAGAAAA+CGG 0.594803 4_3:+60857253 Msa0653030:CDS
GATTCAAAAGAAGTGAAACT+AGG 0.595250 4_3:+60858313 Msa0653030:CDS
CATGTGTGTAACTGTGTCTG+TGG 0.600953 4_3:+60858850 Msa0653030:three_prime_UTR
TGAAAGGAAATCTAATGTGT+TGG 0.601781 4_3:+60859069 Msa0653030:three_prime_UTR
GGTTGAGAATTTGGGCTGAG+AGG 0.603409 4_3:+60858804 Msa0653030:three_prime_UTR
ATTATTGTGATTAGTTAGGG+TGG 0.604892 4_3:+60859017 Msa0653030:three_prime_UTR
TTCAACTAATGATAACTCCA+TGG 0.607734 4_3:+60858153 Msa0653030:CDS
TCTATCTCTGCAGTTGCATG+CGG 0.608651 4_3:+60857042 Msa0653030:five_prime_UTR
ACACTTAGAAACGTTCCTGT+TGG 0.611337 4_3:+60857710 Msa0653030:CDS
TACACACATGATGAGAGAAG+AGG 0.615179 4_3:-60858837 None:intergenic
TAATTGTGGTATAGAAGAGG+GGG 0.616332 4_3:+60859154 Msa0653030:three_prime_UTR
CACTTGTAACAAATTGACAC+AGG 0.623733 4_3:+60857280 Msa0653030:CDS
AAAGAAGTGAAACTAGGTGA+AGG 0.626336 4_3:+60858319 Msa0653030:CDS
AGGCCATCAAGCGAAACTCA+AGG 0.626605 4_3:+60857167 Msa0653030:five_prime_UTR
ATTTGGGCTGAGAGGAACCT+CGG 0.631553 4_3:+60858812 Msa0653030:three_prime_UTR
AATAATATGAATGCTAATGG+TGG 0.635173 4_3:+60858370 Msa0653030:CDS
CTTGAAACATAAAACTGACA+GGG 0.645108 4_3:-60858782 None:intergenic
GCTCAGATCAAGGGATATGG+AGG 0.647696 4_3:+60858874 Msa0653030:three_prime_UTR
TGATGAGAGAAGAGGATCCG+AGG 0.663407 4_3:-60858829 None:intergenic
AATCCAAGACCATTCACCTG+AGG 0.720277 4_3:-60858025 None:intergenic
GTGTTTGATGAATCACAGCG+TGG 0.730885 4_3:-60857602 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAATTAGATATTTTTTTAA+TGG - chr4_3:60857452-60857471 None:intergenic 10.0%
!!! TTTTGTTTTTTATTTTCATT+TGG + chr4_3:60858643-60858662 Msa0653030:three_prime_UTR 10.0%
!!! TTTGTTTTTTATTTTCATTT+GGG + chr4_3:60858644-60858663 Msa0653030:three_prime_UTR 10.0%
!! TAATATTATTTACTTTGATT+TGG + chr4_3:60859181-60859200 Msa0653030:three_prime_UTR 10.0%
!!! AATATTATTTACTTTGATTT+GGG + chr4_3:60859182-60859201 Msa0653030:three_prime_UTR 10.0%
!!! CAAATTAGATATTTTTTTAA+TGG - chr4_3:60857452-60857471 None:intergenic 10.0%
!!! TTTTGTTTTTTATTTTCATT+TGG + chr4_3:60858643-60858662 Msa0653030:three_prime_UTR 10.0%
!!! TTTGTTTTTTATTTTCATTT+GGG + chr4_3:60858644-60858663 Msa0653030:three_prime_UTR 10.0%
!! TAATATTATTTACTTTGATT+TGG + chr4_3:60859181-60859200 Msa0653030:three_prime_UTR 10.0%
!!! AATATTATTTACTTTGATTT+GGG + chr4_3:60859182-60859201 Msa0653030:three_prime_UTR 10.0%
!!! TTGTTTTTTATTTTCATTTG+GGG + chr4_3:60858645-60858664 Msa0653030:three_prime_UTR 15.0%
!! ATAATACATTAGAAAAAGTA+AGG - chr4_3:60859002-60859021 None:intergenic 15.0%
!!! TTGTTTTTTATTTTCATTTG+GGG + chr4_3:60858645-60858664 Msa0653030:three_prime_UTR 15.0%
!! ATAATACATTAGAAAAAGTA+AGG - chr4_3:60859002-60859021 None:intergenic 15.0%
!! AGTAATAATATGAATGCTAA+TGG + chr4_3:60858367-60858386 Msa0653030:CDS 20.0%
!! GAATTATGACCAATATTATT+AGG - chr4_3:60858529-60858548 None:intergenic 20.0%
!! AAGAAAAAGAAGATACTTAA+AGG + chr4_3:60858704-60858723 Msa0653030:three_prime_UTR 20.0%
!! AGAAAAAGTAAGGAAAATAA+AGG - chr4_3:60858992-60859011 None:intergenic 20.0%
!!! ATGTATTATTGTGATTAGTT+AGG + chr4_3:60859013-60859032 Msa0653030:three_prime_UTR 20.0%
!!! TGTATTATTGTGATTAGTTA+GGG + chr4_3:60859014-60859033 Msa0653030:three_prime_UTR 20.0%
!! TTGTTAACTTTCTTAACATT+TGG + chr4_3:60859100-60859119 Msa0653030:three_prime_UTR 20.0%
!! AGTAATAATATGAATGCTAA+TGG + chr4_3:60858367-60858386 Msa0653030:CDS 20.0%
!! GAATTATGACCAATATTATT+AGG - chr4_3:60858529-60858548 None:intergenic 20.0%
!! AAGAAAAAGAAGATACTTAA+AGG + chr4_3:60858704-60858723 Msa0653030:three_prime_UTR 20.0%
!! AGAAAAAGTAAGGAAAATAA+AGG - chr4_3:60858992-60859011 None:intergenic 20.0%
!!! ATGTATTATTGTGATTAGTT+AGG + chr4_3:60859013-60859032 Msa0653030:three_prime_UTR 20.0%
!!! TGTATTATTGTGATTAGTTA+GGG + chr4_3:60859014-60859033 Msa0653030:three_prime_UTR 20.0%
!! TTGTTAACTTTCTTAACATT+TGG + chr4_3:60859100-60859119 Msa0653030:three_prime_UTR 20.0%
!!! AAATTTTGTTGTTTTCCTCT+TGG + chr4_3:60857359-60857378 Msa0653030:intron 25.0%
!!! AAGAAAGAGAAAGTGTTTTA+AGG - chr4_3:60857419-60857438 None:intergenic 25.0%
!!! TTGTTTTTCTTATCCTTTGT+AGG + chr4_3:60857469-60857488 Msa0653030:intron 25.0%
! AGATGATCATAAGATTTCAA+TGG + chr4_3:60857499-60857518 Msa0653030:CDS 25.0%
! TAGAAGCAATATCAAAACTA+TGG - chr4_3:60857871-60857890 None:intergenic 25.0%
! CATAAAGAAGAGAATTGATA+TGG - chr4_3:60857901-60857920 None:intergenic 25.0%
! CATATCAATTCTCTTCTTTA+TGG + chr4_3:60857899-60857918 Msa0653030:CDS 25.0%
! AATAATATGAATGCTAATGG+TGG + chr4_3:60858370-60858389 Msa0653030:CDS 25.0%
! TATGACCAATATTATTAGGT+TGG - chr4_3:60858525-60858544 None:intergenic 25.0%
! TTTACTCACTATATGATGAA+AGG + chr4_3:60859053-60859072 Msa0653030:three_prime_UTR 25.0%
! TAACTTTCTTAACATTTGGA+AGG + chr4_3:60859104-60859123 Msa0653030:three_prime_UTR 25.0%
! AACTTTCTTAACATTTGGAA+GGG + chr4_3:60859105-60859124 Msa0653030:three_prime_UTR 25.0%
! TATAATTGTGGTATAGAAGA+GGG + chr4_3:60859152-60859171 Msa0653030:three_prime_UTR 25.0%
!!! AAATTTTGTTGTTTTCCTCT+TGG + chr4_3:60857359-60857378 Msa0653030:intron 25.0%
!!! AAGAAAGAGAAAGTGTTTTA+AGG - chr4_3:60857419-60857438 None:intergenic 25.0%
!!! TTGTTTTTCTTATCCTTTGT+AGG + chr4_3:60857469-60857488 Msa0653030:intron 25.0%
! AGATGATCATAAGATTTCAA+TGG + chr4_3:60857499-60857518 Msa0653030:CDS 25.0%
! TAGAAGCAATATCAAAACTA+TGG - chr4_3:60857871-60857890 None:intergenic 25.0%
! CATAAAGAAGAGAATTGATA+TGG - chr4_3:60857901-60857920 None:intergenic 25.0%
! CATATCAATTCTCTTCTTTA+TGG + chr4_3:60857899-60857918 Msa0653030:CDS 25.0%
! AATAATATGAATGCTAATGG+TGG + chr4_3:60858370-60858389 Msa0653030:CDS 25.0%
! TATGACCAATATTATTAGGT+TGG - chr4_3:60858525-60858544 None:intergenic 25.0%
! TTTACTCACTATATGATGAA+AGG + chr4_3:60859053-60859072 Msa0653030:three_prime_UTR 25.0%
! TAACTTTCTTAACATTTGGA+AGG + chr4_3:60859104-60859123 Msa0653030:three_prime_UTR 25.0%
! AACTTTCTTAACATTTGGAA+GGG + chr4_3:60859105-60859124 Msa0653030:three_prime_UTR 25.0%
! TATAATTGTGGTATAGAAGA+GGG + chr4_3:60859152-60859171 Msa0653030:three_prime_UTR 25.0%
! CTTTGTTTACTCCATTTCTA+AGG + chr4_3:60857147-60857166 Msa0653030:five_prime_UTR 30.0%
! AACACAGTTACCAAACATTT+TGG - chr4_3:60857243-60857262 None:intergenic 30.0%
! TAACTGTGTTGATGAGAAAA+CGG + chr4_3:60857253-60857272 Msa0653030:CDS 30.0%
GGAAAACAACAAAATTTGAG+TGG - chr4_3:60857356-60857375 None:intergenic 30.0%
!!! AAAGAGAAAGTGTTTTAAGG+TGG - chr4_3:60857416-60857435 None:intergenic 30.0%
! TCTTGTCCAATATCTTTTAC+AGG - chr4_3:60857684-60857703 None:intergenic 30.0%
TGTAAAAGATATTGGACAAG+AGG + chr4_3:60857683-60857702 Msa0653030:CDS 30.0%
ATGTTTGAAGAAGAGTGATT+TGG - chr4_3:60857770-60857789 None:intergenic 30.0%
AAGAGAATTGATATGGTTTG+AGG - chr4_3:60857894-60857913 None:intergenic 30.0%
!! TTGGATTAGGTTTTTCATCT+GGG + chr4_3:60858041-60858060 Msa0653030:CDS 30.0%
CATCATGATGATTACAGAAA+TGG + chr4_3:60858079-60858098 Msa0653030:CDS 30.0%
! GATGATTACAGAAATGGATT+TGG + chr4_3:60858085-60858104 Msa0653030:CDS 30.0%
TTCAACTAATGATAACTCCA+TGG + chr4_3:60858153-60858172 Msa0653030:CDS 30.0%
GATTCAAAAGAAGTGAAACT+AGG + chr4_3:60858313-60858332 Msa0653030:CDS 30.0%
!!! TGTTACTTTTCAAAACCAGA+TGG + chr4_3:60858393-60858412 Msa0653030:CDS 30.0%
CAGATTCTTCTTCTCTTTAT+TGG + chr4_3:60858431-60858450 Msa0653030:CDS 30.0%
GATCACCAACCTAATAATAT+TGG + chr4_3:60858517-60858536 Msa0653030:CDS 30.0%
ATCTAGCTATCTCTTCTATT+AGG + chr4_3:60858597-60858616 Msa0653030:three_prime_UTR 30.0%
!! TATTTTCATTTGGGGTCAAT+TGG + chr4_3:60858653-60858672 Msa0653030:three_prime_UTR 30.0%
!! ATTTTCATTTGGGGTCAATT+GGG + chr4_3:60858654-60858673 Msa0653030:three_prime_UTR 30.0%
CTTGAAACATAAAACTGACA+GGG - chr4_3:60858785-60858804 None:intergenic 30.0%
ATGTTTCAAGGTTGAGAATT+TGG + chr4_3:60858795-60858814 Msa0653030:three_prime_UTR 30.0%
TGTTTCAAGGTTGAGAATTT+GGG + chr4_3:60858796-60858815 Msa0653030:three_prime_UTR 30.0%
CTTACTCAATCAATCAACTA+TGG - chr4_3:60858923-60858942 None:intergenic 30.0%
AGTTGATTGATTGAGTAAGT+TGG + chr4_3:60858924-60858943 Msa0653030:three_prime_UTR 30.0%
! ATTATTGTGATTAGTTAGGG+TGG + chr4_3:60859017-60859036 Msa0653030:three_prime_UTR 30.0%
TGAAAGGAAATCTAATGTGT+TGG + chr4_3:60859069-60859088 Msa0653030:three_prime_UTR 30.0%
ACTTTCTTAACATTTGGAAG+GGG + chr4_3:60859106-60859125 Msa0653030:three_prime_UTR 30.0%
GTATAATTGTGGTATAGAAG+AGG + chr4_3:60859151-60859170 Msa0653030:three_prime_UTR 30.0%
ATAATTGTGGTATAGAAGAG+GGG + chr4_3:60859153-60859172 Msa0653030:three_prime_UTR 30.0%
TTGGGTGATTAATATGTATG+TGG + chr4_3:60859200-60859219 Msa0653030:three_prime_UTR 30.0%
TGGGTGATTAATATGTATGT+GGG + chr4_3:60859201-60859220 Msa0653030:three_prime_UTR 30.0%
GATTAATATGTATGTGGGAT+TGG + chr4_3:60859206-60859225 Msa0653030:three_prime_UTR 30.0%
! CTTTGTTTACTCCATTTCTA+AGG + chr4_3:60857147-60857166 Msa0653030:five_prime_UTR 30.0%
! AACACAGTTACCAAACATTT+TGG - chr4_3:60857243-60857262 None:intergenic 30.0%
! TAACTGTGTTGATGAGAAAA+CGG + chr4_3:60857253-60857272 Msa0653030:CDS 30.0%
GGAAAACAACAAAATTTGAG+TGG - chr4_3:60857356-60857375 None:intergenic 30.0%
!!! AAAGAGAAAGTGTTTTAAGG+TGG - chr4_3:60857416-60857435 None:intergenic 30.0%
! TCTTGTCCAATATCTTTTAC+AGG - chr4_3:60857684-60857703 None:intergenic 30.0%
TGTAAAAGATATTGGACAAG+AGG + chr4_3:60857683-60857702 Msa0653030:CDS 30.0%
ATGTTTGAAGAAGAGTGATT+TGG - chr4_3:60857770-60857789 None:intergenic 30.0%
AAGAGAATTGATATGGTTTG+AGG - chr4_3:60857894-60857913 None:intergenic 30.0%
!! TTGGATTAGGTTTTTCATCT+GGG + chr4_3:60858041-60858060 Msa0653030:CDS 30.0%
CATCATGATGATTACAGAAA+TGG + chr4_3:60858079-60858098 Msa0653030:CDS 30.0%
! GATGATTACAGAAATGGATT+TGG + chr4_3:60858085-60858104 Msa0653030:CDS 30.0%
TTCAACTAATGATAACTCCA+TGG + chr4_3:60858153-60858172 Msa0653030:CDS 30.0%
GATTCAAAAGAAGTGAAACT+AGG + chr4_3:60858313-60858332 Msa0653030:CDS 30.0%
!!! TGTTACTTTTCAAAACCAGA+TGG + chr4_3:60858393-60858412 Msa0653030:CDS 30.0%
CAGATTCTTCTTCTCTTTAT+TGG + chr4_3:60858431-60858450 Msa0653030:CDS 30.0%
GATCACCAACCTAATAATAT+TGG + chr4_3:60858517-60858536 Msa0653030:CDS 30.0%
ATCTAGCTATCTCTTCTATT+AGG + chr4_3:60858597-60858616 Msa0653030:three_prime_UTR 30.0%
!! TATTTTCATTTGGGGTCAAT+TGG + chr4_3:60858653-60858672 Msa0653030:three_prime_UTR 30.0%
!! ATTTTCATTTGGGGTCAATT+GGG + chr4_3:60858654-60858673 Msa0653030:three_prime_UTR 30.0%
CTTGAAACATAAAACTGACA+GGG - chr4_3:60858785-60858804 None:intergenic 30.0%
ATGTTTCAAGGTTGAGAATT+TGG + chr4_3:60858795-60858814 Msa0653030:three_prime_UTR 30.0%
TGTTTCAAGGTTGAGAATTT+GGG + chr4_3:60858796-60858815 Msa0653030:three_prime_UTR 30.0%
CTTACTCAATCAATCAACTA+TGG - chr4_3:60858923-60858942 None:intergenic 30.0%
AGTTGATTGATTGAGTAAGT+TGG + chr4_3:60858924-60858943 Msa0653030:three_prime_UTR 30.0%
! ATTATTGTGATTAGTTAGGG+TGG + chr4_3:60859017-60859036 Msa0653030:three_prime_UTR 30.0%
TGAAAGGAAATCTAATGTGT+TGG + chr4_3:60859069-60859088 Msa0653030:three_prime_UTR 30.0%
ACTTTCTTAACATTTGGAAG+GGG + chr4_3:60859106-60859125 Msa0653030:three_prime_UTR 30.0%
GTATAATTGTGGTATAGAAG+AGG + chr4_3:60859151-60859170 Msa0653030:three_prime_UTR 30.0%
ATAATTGTGGTATAGAAGAG+GGG + chr4_3:60859153-60859172 Msa0653030:three_prime_UTR 30.0%
TTGGGTGATTAATATGTATG+TGG + chr4_3:60859200-60859219 Msa0653030:three_prime_UTR 30.0%
TGGGTGATTAATATGTATGT+GGG + chr4_3:60859201-60859220 Msa0653030:three_prime_UTR 30.0%
GATTAATATGTATGTGGGAT+TGG + chr4_3:60859206-60859225 Msa0653030:three_prime_UTR 30.0%
CTCATAGAGACCAAAATGTT+TGG + chr4_3:60857230-60857249 Msa0653030:exon 35.0%
CACTTGTAACAAATTGACAC+AGG + chr4_3:60857280-60857299 Msa0653030:CDS 35.0%
ATTTCAATGGCTTCTTCAAG+TGG + chr4_3:60857512-60857531 Msa0653030:CDS 35.0%
! TTCTTCAAGTGGTGGAAATA+GGG + chr4_3:60857523-60857542 Msa0653030:CDS 35.0%
AATAGGGTTATGGAAAAACC+AGG + chr4_3:60857539-60857558 Msa0653030:CDS 35.0%
AACAGCAACAACAAGCTTTA+AGG + chr4_3:60857576-60857595 Msa0653030:CDS 35.0%
GCAAAGCCTGTAAAAGATAT+TGG + chr4_3:60857675-60857694 Msa0653030:CDS 35.0%
AAAAGATATTGGACAAGAGG+TGG + chr4_3:60857686-60857705 Msa0653030:CDS 35.0%
! TGAACTTTCCTTTTGCAACA+AGG + chr4_3:60857945-60857964 Msa0653030:CDS 35.0%
GAATTGAACCTTGTTGCAAA+AGG - chr4_3:60857956-60857975 None:intergenic 35.0%
!! ACTAGAGTTTCAAGTTCTTC+TGG + chr4_3:60857977-60857996 Msa0653030:CDS 35.0%
!! CTTGGATTAGGTTTTTCATC+TGG + chr4_3:60858040-60858059 Msa0653030:CDS 35.0%
!! TGGATTAGGTTTTTCATCTG+GGG + chr4_3:60858042-60858061 Msa0653030:CDS 35.0%
AACAACTACAGTTCCATCTT+TGG + chr4_3:60858118-60858137 Msa0653030:CDS 35.0%
AGTAGAAGAAGAACCAAAGA+TGG - chr4_3:60858134-60858153 None:intergenic 35.0%
TTAAGTAGAGAAGCCATAAC+TGG - chr4_3:60858190-60858209 None:intergenic 35.0%
AAAGAAGTGAAACTAGGTGA+AGG + chr4_3:60858319-60858338 Msa0653030:CDS 35.0%
TAGGTGAAGGACTACAAAAT+AGG + chr4_3:60858332-60858351 Msa0653030:CDS 35.0%
TGAAGGACTACAAAATAGGT+TGG + chr4_3:60858336-60858355 Msa0653030:CDS 35.0%
! TACAAAATAGGTTGGATCAG+TGG + chr4_3:60858344-60858363 Msa0653030:CDS 35.0%
TCAAAACCAGATGGAGAATA+TGG + chr4_3:60858402-60858421 Msa0653030:CDS 35.0%
CAAAACCAGATGGAGAATAT+GGG + chr4_3:60858403-60858422 Msa0653030:CDS 35.0%
! TGGGTTTGGTTTACTTACAT+TGG + chr4_3:60858673-60858692 Msa0653030:three_prime_UTR 35.0%
ACTTAAAGGTGAGAGAGTTT+AGG + chr4_3:60858718-60858737 Msa0653030:three_prime_UTR 35.0%
CCTTGAAACATAAAACTGAC+AGG - chr4_3:60858786-60858805 None:intergenic 35.0%
!! CCTGTCAGTTTTATGTTTCA+AGG + chr4_3:60858783-60858802 Msa0653030:three_prime_UTR 35.0%
! GGTTATTGGAGGTATAATTG+TGG + chr4_3:60859140-60859159 Msa0653030:three_prime_UTR 35.0%
TAATTGTGGTATAGAAGAGG+GGG + chr4_3:60859154-60859173 Msa0653030:three_prime_UTR 35.0%
!!! TATGTATGTGGGATTGGTTT+TGG + chr4_3:60859212-60859231 Msa0653030:three_prime_UTR 35.0%
CTCATAGAGACCAAAATGTT+TGG + chr4_3:60857230-60857249 Msa0653030:exon 35.0%
CACTTGTAACAAATTGACAC+AGG + chr4_3:60857280-60857299 Msa0653030:CDS 35.0%
ATTTCAATGGCTTCTTCAAG+TGG + chr4_3:60857512-60857531 Msa0653030:CDS 35.0%
! TTCTTCAAGTGGTGGAAATA+GGG + chr4_3:60857523-60857542 Msa0653030:CDS 35.0%
AATAGGGTTATGGAAAAACC+AGG + chr4_3:60857539-60857558 Msa0653030:CDS 35.0%
AACAGCAACAACAAGCTTTA+AGG + chr4_3:60857576-60857595 Msa0653030:CDS 35.0%
GCAAAGCCTGTAAAAGATAT+TGG + chr4_3:60857675-60857694 Msa0653030:CDS 35.0%
AAAAGATATTGGACAAGAGG+TGG + chr4_3:60857686-60857705 Msa0653030:CDS 35.0%
! TGAACTTTCCTTTTGCAACA+AGG + chr4_3:60857945-60857964 Msa0653030:CDS 35.0%
GAATTGAACCTTGTTGCAAA+AGG - chr4_3:60857956-60857975 None:intergenic 35.0%
!! ACTAGAGTTTCAAGTTCTTC+TGG + chr4_3:60857977-60857996 Msa0653030:CDS 35.0%
!! CTTGGATTAGGTTTTTCATC+TGG + chr4_3:60858040-60858059 Msa0653030:CDS 35.0%
!! TGGATTAGGTTTTTCATCTG+GGG + chr4_3:60858042-60858061 Msa0653030:CDS 35.0%
AACAACTACAGTTCCATCTT+TGG + chr4_3:60858118-60858137 Msa0653030:CDS 35.0%
AGTAGAAGAAGAACCAAAGA+TGG - chr4_3:60858134-60858153 None:intergenic 35.0%
TTAAGTAGAGAAGCCATAAC+TGG - chr4_3:60858190-60858209 None:intergenic 35.0%
AAAGAAGTGAAACTAGGTGA+AGG + chr4_3:60858319-60858338 Msa0653030:CDS 35.0%
TAGGTGAAGGACTACAAAAT+AGG + chr4_3:60858332-60858351 Msa0653030:CDS 35.0%
TGAAGGACTACAAAATAGGT+TGG + chr4_3:60858336-60858355 Msa0653030:CDS 35.0%
! TACAAAATAGGTTGGATCAG+TGG + chr4_3:60858344-60858363 Msa0653030:CDS 35.0%
TCAAAACCAGATGGAGAATA+TGG + chr4_3:60858402-60858421 Msa0653030:CDS 35.0%
CAAAACCAGATGGAGAATAT+GGG + chr4_3:60858403-60858422 Msa0653030:CDS 35.0%
! TGGGTTTGGTTTACTTACAT+TGG + chr4_3:60858673-60858692 Msa0653030:three_prime_UTR 35.0%
ACTTAAAGGTGAGAGAGTTT+AGG + chr4_3:60858718-60858737 Msa0653030:three_prime_UTR 35.0%
CCTTGAAACATAAAACTGAC+AGG - chr4_3:60858786-60858805 None:intergenic 35.0%
!! CCTGTCAGTTTTATGTTTCA+AGG + chr4_3:60858783-60858802 Msa0653030:three_prime_UTR 35.0%
! GGTTATTGGAGGTATAATTG+TGG + chr4_3:60859140-60859159 Msa0653030:three_prime_UTR 35.0%
TAATTGTGGTATAGAAGAGG+GGG + chr4_3:60859154-60859173 Msa0653030:three_prime_UTR 35.0%
!!! TATGTATGTGGGATTGGTTT+TGG + chr4_3:60859212-60859231 Msa0653030:three_prime_UTR 35.0%
AGTTGCATGCGGATGATAAT+CGG + chr4_3:60857053-60857072 Msa0653030:five_prime_UTR 40.0%
TCTTATCATGCTAGCTAGCT+AGG + chr4_3:60857109-60857128 Msa0653030:five_prime_UTR 40.0%
GATATCAGACAAAGAGCAGT+TGG - chr4_3:60857195-60857214 None:intergenic 40.0%
GATAGAGAAAGAGAGTGAGA+TGG - chr4_3:60857308-60857327 None:intergenic 40.0%
TGGCTATATTCTGTTCCAAG+AGG - chr4_3:60857377-60857396 None:intergenic 40.0%
TCATCTATCTCAGCCTACAA+AGG - chr4_3:60857485-60857504 None:intergenic 40.0%
! CTTCTTCAAGTGGTGGAAAT+AGG + chr4_3:60857522-60857541 Msa0653030:CDS 40.0%
! AAGTGGTGGAAATAGGGTTA+TGG + chr4_3:60857529-60857548 Msa0653030:CDS 40.0%
ACACTTAGAAACGTTCCTGT+TGG + chr4_3:60857710-60857729 Msa0653030:CDS 40.0%
CTAGTAGGAGTTGAAGAAGA+AGG - chr4_3:60857812-60857831 None:intergenic 40.0%
GGGTTTGAATTAGCACTAGT+AGG - chr4_3:60857827-60857846 None:intergenic 40.0%
! GTTGTTGTTGTTGAGAAGGA+GGG - chr4_3:60857847-60857866 None:intergenic 40.0%
! TGTTGTTGTTGTTGAGAAGG+AGG - chr4_3:60857848-60857867 None:intergenic 40.0%
!! TGGTGTTGTTGTTGTTGAGA+AGG - chr4_3:60857851-60857870 None:intergenic 40.0%
! ATTAGGTTTTTCATCTGGGG+TGG + chr4_3:60858045-60858064 Msa0653030:CDS 40.0%
! TGGTGATCCTTTTCATCATG+AGG + chr4_3:60858279-60858298 Msa0653030:CDS 40.0%
! ATCCTTTTCATCATGAGGCT+GGG + chr4_3:60858284-60858303 Msa0653030:CDS 40.0%
ACAAACCCATATTCTCCATC+TGG - chr4_3:60858411-60858430 None:intergenic 40.0%
CAACAGGTTTGAGTAGTGTT+TGG + chr4_3:60858488-60858507 Msa0653030:CDS 40.0%
TACACACATGATGAGAGAAG+AGG - chr4_3:60858840-60858859 None:intergenic 40.0%
GGATATGGAGGTTCTTCATT+TGG + chr4_3:60858886-60858905 Msa0653030:three_prime_UTR 40.0%
CTTAACATTTGGAAGGGGTT+AGG + chr4_3:60859111-60859130 Msa0653030:three_prime_UTR 40.0%
ATTTGGAAGGGGTTAGGTTA+TGG + chr4_3:60859117-60859136 Msa0653030:three_prime_UTR 40.0%
TTTGGAAGGGGTTAGGTTAT+GGG + chr4_3:60859118-60859137 Msa0653030:three_prime_UTR 40.0%
TTAGGTTATGGGGTTATTGG+AGG + chr4_3:60859129-60859148 Msa0653030:three_prime_UTR 40.0%
!!! GTGGGATTGGTTTTGGTATT+TGG + chr4_3:60859219-60859238 Msa0653030:three_prime_UTR 40.0%
!!! TGGTTTTGGTATTTGGTGTG+TGG + chr4_3:60859226-60859245 Msa0653030:three_prime_UTR 40.0%
AGTTGCATGCGGATGATAAT+CGG + chr4_3:60857053-60857072 Msa0653030:five_prime_UTR 40.0%
TCTTATCATGCTAGCTAGCT+AGG + chr4_3:60857109-60857128 Msa0653030:five_prime_UTR 40.0%
GATATCAGACAAAGAGCAGT+TGG - chr4_3:60857195-60857214 None:intergenic 40.0%
GATAGAGAAAGAGAGTGAGA+TGG - chr4_3:60857308-60857327 None:intergenic 40.0%
TGGCTATATTCTGTTCCAAG+AGG - chr4_3:60857377-60857396 None:intergenic 40.0%
TCATCTATCTCAGCCTACAA+AGG - chr4_3:60857485-60857504 None:intergenic 40.0%
! CTTCTTCAAGTGGTGGAAAT+AGG + chr4_3:60857522-60857541 Msa0653030:CDS 40.0%
! AAGTGGTGGAAATAGGGTTA+TGG + chr4_3:60857529-60857548 Msa0653030:CDS 40.0%
ACACTTAGAAACGTTCCTGT+TGG + chr4_3:60857710-60857729 Msa0653030:CDS 40.0%
CTAGTAGGAGTTGAAGAAGA+AGG - chr4_3:60857812-60857831 None:intergenic 40.0%
GGGTTTGAATTAGCACTAGT+AGG - chr4_3:60857827-60857846 None:intergenic 40.0%
! GTTGTTGTTGTTGAGAAGGA+GGG - chr4_3:60857847-60857866 None:intergenic 40.0%
! TGTTGTTGTTGTTGAGAAGG+AGG - chr4_3:60857848-60857867 None:intergenic 40.0%
!! TGGTGTTGTTGTTGTTGAGA+AGG - chr4_3:60857851-60857870 None:intergenic 40.0%
! ATTAGGTTTTTCATCTGGGG+TGG + chr4_3:60858045-60858064 Msa0653030:CDS 40.0%
! TGGTGATCCTTTTCATCATG+AGG + chr4_3:60858279-60858298 Msa0653030:CDS 40.0%
! ATCCTTTTCATCATGAGGCT+GGG + chr4_3:60858284-60858303 Msa0653030:CDS 40.0%
ACAAACCCATATTCTCCATC+TGG - chr4_3:60858411-60858430 None:intergenic 40.0%
CAACAGGTTTGAGTAGTGTT+TGG + chr4_3:60858488-60858507 Msa0653030:CDS 40.0%
TACACACATGATGAGAGAAG+AGG - chr4_3:60858840-60858859 None:intergenic 40.0%
GGATATGGAGGTTCTTCATT+TGG + chr4_3:60858886-60858905 Msa0653030:three_prime_UTR 40.0%
CTTAACATTTGGAAGGGGTT+AGG + chr4_3:60859111-60859130 Msa0653030:three_prime_UTR 40.0%
ATTTGGAAGGGGTTAGGTTA+TGG + chr4_3:60859117-60859136 Msa0653030:three_prime_UTR 40.0%
TTTGGAAGGGGTTAGGTTAT+GGG + chr4_3:60859118-60859137 Msa0653030:three_prime_UTR 40.0%
TTAGGTTATGGGGTTATTGG+AGG + chr4_3:60859129-60859148 Msa0653030:three_prime_UTR 40.0%
!!! GTGGGATTGGTTTTGGTATT+TGG + chr4_3:60859219-60859238 Msa0653030:three_prime_UTR 40.0%
!!! TGGTTTTGGTATTTGGTGTG+TGG + chr4_3:60859226-60859245 Msa0653030:three_prime_UTR 40.0%
TCTATCTCTGCAGTTGCATG+CGG + chr4_3:60857042-60857061 Msa0653030:five_prime_UTR 45.0%
! TCGCTTGATGGCCTTAGAAA+TGG - chr4_3:60857161-60857180 None:intergenic 45.0%
!!! GTGGTGCATGTTTTAGTCAG+TGG - chr4_3:60857397-60857416 None:intergenic 45.0%
TCAATGGCTTCTTCAAGTGG+TGG + chr4_3:60857515-60857534 Msa0653030:CDS 45.0%
TGTTGAAGAAGCTCTTGACC+TGG - chr4_3:60857560-60857579 None:intergenic 45.0%
GTGTTTGATGAATCACAGCG+TGG - chr4_3:60857605-60857624 None:intergenic 45.0%
CTTAGAAACGTTCCTGTTGG+TGG + chr4_3:60857713-60857732 Msa0653030:CDS 45.0%
AATCCAAGACCATTCACCTG+AGG - chr4_3:60858028-60858047 None:intergenic 45.0%
! CAGGTGAATGGTCTTGGATT+AGG + chr4_3:60858028-60858047 Msa0653030:CDS 45.0%
AGCCATAACTGGAGTAGTAG+AGG - chr4_3:60858179-60858198 None:intergenic 45.0%
! GATCCTTTTCATCATGAGGC+TGG + chr4_3:60858283-60858302 Msa0653030:CDS 45.0%
ATCCCAGCCTCATGATGAAA+AGG - chr4_3:60858289-60858308 None:intergenic 45.0%
TCATCATGAGGCTGGGATAA+TGG + chr4_3:60858291-60858310 Msa0653030:CDS 45.0%
! TGCTGCAGTGTTGTTGTTGT+TGG - chr4_3:60858470-60858489 None:intergenic 45.0%
AACAACAACACTGCAGCAAC+AGG + chr4_3:60858472-60858491 Msa0653030:CDS 45.0%
! CATTTGGGGTCAATTGGGTT+TGG + chr4_3:60858659-60858678 Msa0653030:three_prime_UTR 45.0%
CATGTGTGTAACTGTGTCTG+TGG + chr4_3:60858850-60858869 Msa0653030:three_prime_UTR 45.0%
! TGTGATTAGTTAGGGTGGTG+AGG + chr4_3:60859022-60859041 Msa0653030:three_prime_UTR 45.0%
ATTAGTTAGGGTGGTGAGGT+TGG + chr4_3:60859026-60859045 Msa0653030:three_prime_UTR 45.0%
TTAGTTAGGGTGGTGAGGTT+GGG + chr4_3:60859027-60859046 Msa0653030:three_prime_UTR 45.0%
TTGGAAGGGGTTAGGTTATG+GGG + chr4_3:60859119-60859138 Msa0653030:three_prime_UTR 45.0%
GGGTTAGGTTATGGGGTTAT+TGG + chr4_3:60859126-60859145 Msa0653030:three_prime_UTR 45.0%
TCTATCTCTGCAGTTGCATG+CGG + chr4_3:60857042-60857061 Msa0653030:five_prime_UTR 45.0%
! TCGCTTGATGGCCTTAGAAA+TGG - chr4_3:60857161-60857180 None:intergenic 45.0%
!!! GTGGTGCATGTTTTAGTCAG+TGG - chr4_3:60857397-60857416 None:intergenic 45.0%
TCAATGGCTTCTTCAAGTGG+TGG + chr4_3:60857515-60857534 Msa0653030:CDS 45.0%
TGTTGAAGAAGCTCTTGACC+TGG - chr4_3:60857560-60857579 None:intergenic 45.0%
GTGTTTGATGAATCACAGCG+TGG - chr4_3:60857605-60857624 None:intergenic 45.0%
CTTAGAAACGTTCCTGTTGG+TGG + chr4_3:60857713-60857732 Msa0653030:CDS 45.0%
AATCCAAGACCATTCACCTG+AGG - chr4_3:60858028-60858047 None:intergenic 45.0%
! CAGGTGAATGGTCTTGGATT+AGG + chr4_3:60858028-60858047 Msa0653030:CDS 45.0%
AGCCATAACTGGAGTAGTAG+AGG - chr4_3:60858179-60858198 None:intergenic 45.0%
! GATCCTTTTCATCATGAGGC+TGG + chr4_3:60858283-60858302 Msa0653030:CDS 45.0%
ATCCCAGCCTCATGATGAAA+AGG - chr4_3:60858289-60858308 None:intergenic 45.0%
TCATCATGAGGCTGGGATAA+TGG + chr4_3:60858291-60858310 Msa0653030:CDS 45.0%
! TGCTGCAGTGTTGTTGTTGT+TGG - chr4_3:60858470-60858489 None:intergenic 45.0%
AACAACAACACTGCAGCAAC+AGG + chr4_3:60858472-60858491 Msa0653030:CDS 45.0%
! CATTTGGGGTCAATTGGGTT+TGG + chr4_3:60858659-60858678 Msa0653030:three_prime_UTR 45.0%
CATGTGTGTAACTGTGTCTG+TGG + chr4_3:60858850-60858869 Msa0653030:three_prime_UTR 45.0%
! TGTGATTAGTTAGGGTGGTG+AGG + chr4_3:60859022-60859041 Msa0653030:three_prime_UTR 45.0%
ATTAGTTAGGGTGGTGAGGT+TGG + chr4_3:60859026-60859045 Msa0653030:three_prime_UTR 45.0%
TTAGTTAGGGTGGTGAGGTT+GGG + chr4_3:60859027-60859046 Msa0653030:three_prime_UTR 45.0%
TTGGAAGGGGTTAGGTTATG+GGG + chr4_3:60859119-60859138 Msa0653030:three_prime_UTR 45.0%
GGGTTAGGTTATGGGGTTAT+TGG + chr4_3:60859126-60859145 Msa0653030:three_prime_UTR 45.0%
AGGCCATCAAGCGAAACTCA+AGG + chr4_3:60857167-60857186 Msa0653030:five_prime_UTR 50.0%
!! CAGGCTTTGCAGAAGTGTCT+AGG - chr4_3:60857665-60857684 None:intergenic 50.0%
! AGAAACGTTCCTGTTGGTGG+TGG + chr4_3:60857716-60857735 Msa0653030:CDS 50.0%
TTTCTGCAACCACCACCAAC+AGG - chr4_3:60857728-60857747 None:intergenic 50.0%
AACTGGAGTAGTAGAGGCCA+TGG - chr4_3:60858173-60858192 None:intergenic 50.0%
GGCCTCTACTACTCCAGTTA+TGG + chr4_3:60858174-60858193 Msa0653030:CDS 50.0%
GGTTGAGAATTTGGGCTGAG+AGG + chr4_3:60858804-60858823 Msa0653030:three_prime_UTR 50.0%
ATTTGGGCTGAGAGGAACCT+CGG + chr4_3:60858812-60858831 Msa0653030:three_prime_UTR 50.0%
TGATGAGAGAAGAGGATCCG+AGG - chr4_3:60858832-60858851 None:intergenic 50.0%
TGTCTGTGGAGCTCAGATCA+AGG + chr4_3:60858864-60858883 Msa0653030:three_prime_UTR 50.0%
GTCTGTGGAGCTCAGATCAA+GGG + chr4_3:60858865-60858884 Msa0653030:three_prime_UTR 50.0%
GGAGCTCAGATCAAGGGATA+TGG + chr4_3:60858871-60858890 Msa0653030:three_prime_UTR 50.0%
GCTCAGATCAAGGGATATGG+AGG + chr4_3:60858874-60858893 Msa0653030:three_prime_UTR 50.0%
TAGTTAGGGTGGTGAGGTTG+GGG + chr4_3:60859028-60859047 Msa0653030:three_prime_UTR 50.0%
TTGTGGTATAGAAGAGGGGG+AGG + chr4_3:60859157-60859176 Msa0653030:three_prime_UTR 50.0%
AGGCCATCAAGCGAAACTCA+AGG + chr4_3:60857167-60857186 Msa0653030:five_prime_UTR 50.0%
!! CAGGCTTTGCAGAAGTGTCT+AGG - chr4_3:60857665-60857684 None:intergenic 50.0%
! AGAAACGTTCCTGTTGGTGG+TGG + chr4_3:60857716-60857735 Msa0653030:CDS 50.0%
TTTCTGCAACCACCACCAAC+AGG - chr4_3:60857728-60857747 None:intergenic 50.0%
AACTGGAGTAGTAGAGGCCA+TGG - chr4_3:60858173-60858192 None:intergenic 50.0%
GGCCTCTACTACTCCAGTTA+TGG + chr4_3:60858174-60858193 Msa0653030:CDS 50.0%
GGTTGAGAATTTGGGCTGAG+AGG + chr4_3:60858804-60858823 Msa0653030:three_prime_UTR 50.0%
ATTTGGGCTGAGAGGAACCT+CGG + chr4_3:60858812-60858831 Msa0653030:three_prime_UTR 50.0%
TGATGAGAGAAGAGGATCCG+AGG - chr4_3:60858832-60858851 None:intergenic 50.0%
TGTCTGTGGAGCTCAGATCA+AGG + chr4_3:60858864-60858883 Msa0653030:three_prime_UTR 50.0%
GTCTGTGGAGCTCAGATCAA+GGG + chr4_3:60858865-60858884 Msa0653030:three_prime_UTR 50.0%
GGAGCTCAGATCAAGGGATA+TGG + chr4_3:60858871-60858890 Msa0653030:three_prime_UTR 50.0%
GCTCAGATCAAGGGATATGG+AGG + chr4_3:60858874-60858893 Msa0653030:three_prime_UTR 50.0%
TAGTTAGGGTGGTGAGGTTG+GGG + chr4_3:60859028-60859047 Msa0653030:three_prime_UTR 50.0%
TTGTGGTATAGAAGAGGGGG+AGG + chr4_3:60859157-60859176 Msa0653030:three_prime_UTR 50.0%
GCGCCTTGAGTTTCGCTTGA+TGG - chr4_3:60857173-60857192 None:intergenic 55.0%
CAGCCTCAGGTGAATGGTCT+TGG + chr4_3:60858022-60858041 Msa0653030:CDS 55.0%
!! GCTGGTGGTGGTGGAATGAA+AGG + chr4_3:60858256-60858275 Msa0653030:CDS 55.0%
!! GGTGGTGGTGGAATGAAAGG+TGG + chr4_3:60858259-60858278 Msa0653030:CDS 55.0%
AGTTAGGGTGGTGAGGTTGG+GGG + chr4_3:60859029-60859048 Msa0653030:three_prime_UTR 55.0%
GCGCCTTGAGTTTCGCTTGA+TGG - chr4_3:60857173-60857192 None:intergenic 55.0%
CAGCCTCAGGTGAATGGTCT+TGG + chr4_3:60858022-60858041 Msa0653030:CDS 55.0%
!! GCTGGTGGTGGTGGAATGAA+AGG + chr4_3:60858256-60858275 Msa0653030:CDS 55.0%
!! GGTGGTGGTGGAATGAAAGG+TGG + chr4_3:60858259-60858278 Msa0653030:CDS 55.0%
AGTTAGGGTGGTGAGGTTGG+GGG + chr4_3:60859029-60859048 Msa0653030:three_prime_UTR 55.0%
TCTTCAGCAGCAGCAGCCTC+AGG + chr4_3:60858009-60858028 Msa0653030:CDS 60.0%
CAGCAGCAGCCTCAGGTGAA+TGG + chr4_3:60858016-60858035 Msa0653030:CDS 60.0%
AGTACAGCTGCTGCTGCTGC+TGG + chr4_3:60858238-60858257 Msa0653030:CDS 60.0%
TCTTCAGCAGCAGCAGCCTC+AGG + chr4_3:60858009-60858028 Msa0653030:CDS 60.0%
CAGCAGCAGCCTCAGGTGAA+TGG + chr4_3:60858016-60858035 Msa0653030:CDS 60.0%
AGTACAGCTGCTGCTGCTGC+TGG + chr4_3:60858238-60858257 Msa0653030:CDS 60.0%
ACAGCTGCTGCTGCTGCTGG+TGG + chr4_3:60858241-60858260 Msa0653030:CDS 65.0%
ACAGCTGCTGCTGCTGCTGG+TGG + chr4_3:60858241-60858260 Msa0653030:CDS 65.0%
GCTGCTGCTGCTGCTGGTGG+TGG + chr4_3:60858244-60858263 Msa0653030:CDS 70.0%
!! GCTGCTGCTGCTGGTGGTGG+TGG + chr4_3:60858247-60858266 Msa0653030:CDS 70.0%
GCTGCTGCTGCTGCTGGTGG+TGG + chr4_3:60858244-60858263 Msa0653030:CDS 70.0%
!! GCTGCTGCTGCTGGTGGTGG+TGG + chr4_3:60858247-60858266 Msa0653030:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 60857021 60859284 60857021 ID=Msa0653030;Name=Msa0653030
chr4_3 mRNA 60857021 60859284 60857021 ID=Msa0653030-mRNA-1;Parent=Msa0653030;Name=Msa0653030-mRNA-1;_AED=0.00;_eAED=0.00;_QI=224|1|1|1|0|0.5|2|717|377
chr4_3 exon 60857021 60857301 60857021 ID=Msa0653030-mRNA-1:exon:16552;Parent=Msa0653030-mRNA-1
chr4_3 exon 60857491 60859284 60857491 ID=Msa0653030-mRNA-1:exon:16553;Parent=Msa0653030-mRNA-1
chr4_3 five_prime_UTR 60857021 60857244 60857021 ID=Msa0653030-mRNA-1:five_prime_utr;Parent=Msa0653030-mRNA-1
chr4_3 CDS 60857245 60857301 60857245 ID=Msa0653030-mRNA-1:cds;Parent=Msa0653030-mRNA-1
chr4_3 CDS 60857491 60858567 60857491 ID=Msa0653030-mRNA-1:cds;Parent=Msa0653030-mRNA-1
chr4_3 three_prime_UTR 60858568 60859284 60858568 ID=Msa0653030-mRNA-1:three_prime_utr;Parent=Msa0653030-mRNA-1
Gene Sequence

>Msa0653030

ATGTTTGGTAACTGTGTTGATGAGAAAACGGTAATCACTTGTAACAAATTGACACAGGCTGAGATAGATGATCATAAGATTTCAATGGCTTCTTCAAGTGGTGGAAATAGGGTTATGGAAAAACCAGGTCAAGAGCTTCTTCAACAGCAACAACAAGCTTTAAGGTGTCCACGCTGTGATTCATCAAACACTAAATTCTGTTACTACAATAACTACAGTTTATCACAACCTAGACACTTCTGCAAAGCCTGTAAAAGATATTGGACAAGAGGTGGAACACTTAGAAACGTTCCTGTTGGTGGTGGTTGCAGAAAAAACAAACGTCTTAAACGACCAAATCACTCTTCTTCAAACATTGATACTGCTACTGCTTCTCCTTCTTCTTCAACTCCTACTAGTGCTAATTCAAACCCTCCTTCTCAACAACAACAACACCATAGTTTTGATATTGCTTCTACCTCAAACCATATCAATTCTCTTCTTTATGGTAACTCTTCTTGTCATGATGTGATGAACTTTCCTTTTGCAACAAGGTTCAATTCTACTAGAGTTTCAAGTTCTTCTGGTTATGATCATCTTCAGCAGCAGCAGCCTCAGGTGAATGGTCTTGGATTAGGTTTTTCATCTGGGGTGGTTAATCTTAATCATCATGATGATTACAGAAATGGATTTGGTTCAAATAATAACAACTACAGTTCCATCTTTGGTTCTTCTTCTACTTCAACTAATGATAACTCCATGGCCTCTACTACTCCAGTTATGGCTTCTCTACTTAACTCTACACTACTACAACAGAAATTCATGAGTACAGCTGCTGCTGCTGCTGGTGGTGGTGGAATGAAAGGTGGTGATCCTTTTCATCATGAGGCTGGGATAATGGATTCAAAAGAAGTGAAACTAGGTGAAGGACTACAAAATAGGTTGGATCAGTGGAGTAATAATATGAATGCTAATGGTGGTGTTACTTTTCAAAACCAGATGGAGAATATGGGTTTGTCAGATTCTTCTTCTCTTTATTGGAATAACAACAACACCAACAACAACAACACTGCAGCAACAGGTTTGAGTAGTGTTTGGAGTATTGATCACCAACCTAATAATATTGGTCATAATTCAGTTACTTCTCTGATCTAG
Protein sequence

>Msa0653030

MFGNCVDEKTVITCNKLTQAEIDDHKISMASSSGGNRVMEKPGQELLQQQQQALRCPRCDSSNTKFCYYNNYSLSQPRHFCKACKRYWTRGGTLRNVPVGGGCRKNKRLKRPNHSSSNIDTATASPSSSTPTSANSNPPSQQQQHHSFDIASTSNHINSLLYGNSSCHDVMNFPFATRFNSTRVSSSSGYDHLQQQQPQVNGLGLGFSSGVVNLNHHDDYRNGFGSNNNNYSSIFGSSSTSTNDNSMASTTPVMASLLNSTLLQQKFMSTAAAAAGGGGMKGGDPFHHEAGIMDSKEVKLGEGLQNRLDQWSNNMNANGGVTFQNQMENMGLSDSSSLYWNNNNTNNNNTAATGLSSVWSIDHQPNNIGHNSVTSLI*