AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0654090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0654090 MtrunA17_Chr4g0048841 99.160 119 1 0 5 123 44 162 7.40e-82 239
Msa0654090 MtrunA17_Chr2g0318961 81.967 122 22 0 1 122 5 126 2.33e-70 209
Msa0654090 MtrunA17_Chr8g0361451 81.148 122 23 0 1 122 5 126 4.19e-69 206
Msa0654090 MtrunA17_Chr2g0280641 69.106 123 38 0 1 123 5 127 5.85e-59 180
Msa0654090 MtrunA17_Chr5g0429671 61.789 123 46 1 1 122 6 128 8.93e-50 157
Msa0654090 MtrunA17_Chr5g0403681 61.667 120 45 1 4 122 57 176 1.72e-47 152
Msa0654090 MtrunA17_Chr4g0034601 60.976 123 47 1 1 122 53 175 4.00e-46 149
Msa0654090 MtrunA17_Chr8g0361461 86.047 43 6 0 80 122 1 43 8.87e-20 78.2
Msa0654090 MtrunA17_Chr4g0048831 88.636 44 1 1 80 123 1 40 1.24e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0654090 AT5G54500.2 84.746 118 18 0 5 122 9 126 3.06e-69 208
Msa0654090 AT5G54500.1 81.967 122 22 0 1 122 5 126 3.42e-69 206
Msa0654090 AT4G27270.1 81.967 122 22 0 1 122 5 126 3.46e-69 206
Msa0654090 AT4G27270.3 84.348 115 18 0 8 122 1 115 4.52e-66 198
Msa0654090 AT4G27270.2 84.348 115 18 0 8 122 1 115 4.52e-66 198
Msa0654090 AT5G58800.1 58.537 123 50 1 1 122 6 128 1.29e-47 152
Msa0654090 AT5G58800.2 58.537 123 50 1 1 122 6 128 1.29e-47 152
Msa0654090 AT4G36750.1 64.167 120 42 1 4 122 78 197 3.28e-47 153
Msa0654090 AT4G27270.4 86.667 75 10 0 48 122 1 75 6.03e-42 136

Find 67 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTATGAAAATGTAAAATA+TGG 0.224334 4_3:+62395597 Msa0654090:five_prime_UTR
CGCTACCTTCTTGAATAAAA+AGG 0.230092 4_3:-62395375 None:intergenic
CTTGGCTTCCCGACAAGATT+TGG 0.247346 4_3:+62396409 Msa0654090:CDS
TATTTGAATATATGTTTCTT+TGG 0.254023 4_3:+62395554 Msa0654090:five_prime_UTR
ACCTCCGCAGGCAGAGTTTC+AGG 0.301695 4_3:-62396304 None:intergenic
AATATGGAAGCTTAATTGAT+TGG 0.318991 4_3:+62395613 Msa0654090:five_prime_UTR
CTCTGCCTGCGGAGGTCGTT+GGG 0.320468 4_3:+62396311 Msa0654090:CDS
AAGAAATAATTGAGATGGTT+GGG 0.328896 4_3:-62395578 None:intergenic
ATAATTGGCACATCACTCTT+TGG 0.334809 4_3:-62396349 None:intergenic
GAGGCTGATGGTTTGTTGCT+TGG 0.342120 4_3:+62396391 Msa0654090:CDS
AGCGCATCAGGTGTAATAAT+TGG 0.347422 4_3:-62396364 None:intergenic
TGGCGTACTCAGGCACTTGC+AGG 0.358990 4_3:+62396481 Msa0654090:CDS
TGCTCAGTTTAAAGCATTCC+TGG 0.369486 4_3:+62396441 Msa0654090:CDS
CTTTGCTTGCAATTGTGATA+AGG 0.375845 4_3:+62395524 Msa0654090:five_prime_UTR
TAGCTTATATGAAAACAGTT+TGG 0.390186 4_3:-62395317 None:intergenic
TGCGGAGGTCGTTGGGAAGA+TGG 0.403064 4_3:+62396318 Msa0654090:CDS
TATGCATCAACAGAGGAATA+TGG 0.410667 4_3:-62395723 None:intergenic
TTTACAACCTTCTACTGAAA+AGG 0.432802 4_3:-62395420 None:intergenic
TCAGCCTCAGGTAGCGCATC+AGG 0.442003 4_3:-62396376 None:intergenic
CATCACCAATGATGGATGAT+AGG 0.443259 4_3:-62395494 None:intergenic
TAAGAAATAATTGAGATGGT+TGG 0.446949 4_3:-62395579 None:intergenic
GCGGAGGTCGTTGGGAAGAT+GGG 0.453021 4_3:+62396319 Msa0654090:CDS
AAAGCATTCCTGGATGCAAC+TGG 0.456257 4_3:+62396451 Msa0654090:CDS
GGAAGAGTCATCACCAATGA+TGG 0.465121 4_3:-62395502 None:intergenic
CCCGACAAGATTTGGAATGA+TGG 0.467275 4_3:+62396417 Msa0654090:CDS
CTTAGGAATAACTGTAAGAA+TGG 0.484968 4_3:-62395134 None:intergenic
TTCCTCTGTTGATGCATAAG+TGG 0.491557 4_3:+62395728 Msa0654090:exon
CCATCATTCCAAATCTTGTC+GGG 0.494553 4_3:-62396417 None:intergenic
TTTAGTTACTATTCTATGTA+CGG 0.496466 4_3:+62395965 Msa0654090:intron
ATTCTCTAATATAATTTCAT+CGG 0.497213 4_3:-62395050 None:intergenic
TTCATAAGAAATAATTGAGA+TGG 0.498728 4_3:-62395583 None:intergenic
GCCATCATTCCAAATCTTGT+CGG 0.505362 4_3:-62396418 None:intergenic
ATCTTCCCAACGACCTCCGC+AGG 0.515536 4_3:-62396316 None:intergenic
AGTATGCACTCTTGTCTACA+TGG 0.518840 4_3:-62395455 None:intergenic
ACTCTGCCTGCGGAGGTCGT+TGG 0.519015 4_3:+62396310 Msa0654090:CDS
ACAACCTTCTACTGAAAAGG+TGG 0.529121 4_3:-62395417 None:intergenic
CTTTGCTTCTACTCCTTCCA+CGG 0.530210 4_3:-62396032 None:intergenic
ATTGGCACATCACTCTTTGG+AGG 0.535259 4_3:-62396346 None:intergenic
AGTAGAAGCAAAGCTATGGC+AGG 0.545320 4_3:+62396042 Msa0654090:CDS
GTGGTCCTATCATCCATCAT+TGG 0.545335 4_3:+62395489 Msa0654090:five_prime_UTR
AGAAATAATTGAGATGGTTG+GGG 0.546733 4_3:-62395577 None:intergenic
AGCAACAAACCATCAGCCTC+AGG 0.546743 4_3:-62396388 None:intergenic
AAAGGGGCGGCCTCCGTGGA+AGG 0.550707 4_3:+62396019 Msa0654090:CDS
TGGATGCAACTGGTGGCCTA+TGG 0.551588 4_3:+62396461 Msa0654090:CDS
GCACTTGCAGGAAAGCCTGC+AGG 0.551589 4_3:+62396493 Msa0654090:CDS
GTGCTGTAAAAGATTCCTGC+AGG 0.556948 4_3:-62396508 None:intergenic
ATAGGCCACCAGTTGCATCC+AGG 0.570687 4_3:-62396459 None:intergenic
AAGGAGTAGAAGCAAAGCTA+TGG 0.577868 4_3:+62396038 Msa0654090:CDS
TGGTGGCCTATGGCGTACTC+AGG 0.578013 4_3:+62396471 Msa0654090:CDS
TTTAAAAGGGGCGGCCTCCG+TGG 0.580700 4_3:+62396015 Msa0654090:CDS
CTTATCACAATTGCAAGCAA+AGG 0.585884 4_3:-62395523 None:intergenic
CAAGTGCCTGAGTACGCCAT+AGG 0.599625 4_3:-62396477 None:intergenic
GAATAACTGTAAGAATGGAT+AGG 0.599789 4_3:-62395129 None:intergenic
TACAGCACTGGTTCTCAAGG+AGG 0.602179 4_3:+62396523 Msa0654090:CDS
TACTAGCAGACAAAGCATGA+TGG 0.603081 4_3:-62395667 None:intergenic
GAGGTCGTTGGGAAGATGGG+AGG 0.615091 4_3:+62396322 Msa0654090:CDS
ACCTGAAACTCTGCCTGCGG+AGG 0.615512 4_3:+62396303 Msa0654090:CDS
AGCACTGGTTCTCAAGGAGG+AGG 0.618456 4_3:+62396526 Msa0654090:CDS
GATGCGCTACCTGAGGCTGA+TGG 0.631007 4_3:+62396379 Msa0654090:CDS
GTAAGCTGTTTATCTGAACA+CGG 0.635264 4_3:-62395211 None:intergenic
CAACCTTCTACTGAAAAGGT+GGG 0.637658 4_3:-62395416 None:intergenic
GCATTCCTGGATGCAACTGG+TGG 0.654481 4_3:+62396454 Msa0654090:CDS
TACACCTGATGCGCTACCTG+AGG 0.657213 4_3:+62396372 Msa0654090:CDS
TGCTTCTACTCCTTCCACGG+AGG 0.674111 4_3:-62396029 None:intergenic
ATCAACAGAGGAATATGGTG+TGG 0.674630 4_3:-62395718 None:intergenic
GGTACCTGAAACTCTGCCTG+CGG 0.684253 4_3:+62396300 Msa0654090:intron
TACCACTTATGCATCAACAG+AGG 0.711718 4_3:-62395730 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTGAACATTTTAATT+TGG - chr4_3:62396168-62396187 None:intergenic 10.0%
!!! TTATTTTGAACATTTTAATT+TGG - chr4_3:62396168-62396187 None:intergenic 10.0%
!!! ATAATTTTACTTATTTCTGT+TGG + chr4_3:62395016-62395035 Msa0654090:five_prime_UTR 15.0%
!! ATTCTCTAATATAATTTCAT+CGG - chr4_3:62395053-62395072 None:intergenic 15.0%
!! TATTTGAATATATGTTTCTT+TGG + chr4_3:62395554-62395573 Msa0654090:five_prime_UTR 15.0%
!! TCTTATGAAAATGTAAAATA+TGG + chr4_3:62395597-62395616 Msa0654090:five_prime_UTR 15.0%
!! ATAAAATAATTTATGTTGCT+TGG + chr4_3:62395844-62395863 Msa0654090:intron 15.0%
!!! ATAATTTTACTTATTTCTGT+TGG + chr4_3:62395016-62395035 Msa0654090:five_prime_UTR 15.0%
!! ATTCTCTAATATAATTTCAT+CGG - chr4_3:62395053-62395072 None:intergenic 15.0%
!! TATTTGAATATATGTTTCTT+TGG + chr4_3:62395554-62395573 Msa0654090:five_prime_UTR 15.0%
!! TCTTATGAAAATGTAAAATA+TGG + chr4_3:62395597-62395616 Msa0654090:five_prime_UTR 15.0%
!! ATAAAATAATTTATGTTGCT+TGG + chr4_3:62395844-62395863 Msa0654090:intron 15.0%
!! TTCATAAGAAATAATTGAGA+TGG - chr4_3:62395586-62395605 None:intergenic 20.0%
!! TTTAGTTACTATTCTATGTA+CGG + chr4_3:62395965-62395984 Msa0654090:intron 20.0%
!! TTCATAAGAAATAATTGAGA+TGG - chr4_3:62395586-62395605 None:intergenic 20.0%
!! TTTAGTTACTATTCTATGTA+CGG + chr4_3:62395965-62395984 Msa0654090:intron 20.0%
! AAAAAAAATGTTTGAGCATG+AGG + chr4_3:62395085-62395104 Msa0654090:five_prime_UTR 25.0%
! TAGCTTATATGAAAACAGTT+TGG - chr4_3:62395320-62395339 None:intergenic 25.0%
!! CTTTTCCTTTTTATTCAAGA+AGG + chr4_3:62395370-62395389 Msa0654090:five_prime_UTR 25.0%
! AAGAAATAATTGAGATGGTT+GGG - chr4_3:62395581-62395600 None:intergenic 25.0%
! TAAGAAATAATTGAGATGGT+TGG - chr4_3:62395582-62395601 None:intergenic 25.0%
! AATATGGAAGCTTAATTGAT+TGG + chr4_3:62395613-62395632 Msa0654090:five_prime_UTR 25.0%
! TATAGCTTTCATAAGTCTTA+TGG - chr4_3:62395880-62395899 None:intergenic 25.0%
!!! TAGCTCAAGAGATTTTAAAA+GGG + chr4_3:62396002-62396021 Msa0654090:CDS 25.0%
! TCAATCAATCTTTACATGTT+TGG + chr4_3:62396132-62396151 Msa0654090:intron 25.0%
!! ACCATTAGATATCTTTAAGA+TGG + chr4_3:62396187-62396206 Msa0654090:intron 25.0%
! ACCATCTTAAAGATATCTAA+TGG - chr4_3:62396191-62396210 None:intergenic 25.0%
!! GATGATAATTTTGTAAATGC+AGG - chr4_3:62396269-62396288 None:intergenic 25.0%
! AATTATCATCTGATACGTAT+AGG + chr4_3:62396279-62396298 Msa0654090:intron 25.0%
! AAAAAAAATGTTTGAGCATG+AGG + chr4_3:62395085-62395104 Msa0654090:five_prime_UTR 25.0%
! TAGCTTATATGAAAACAGTT+TGG - chr4_3:62395320-62395339 None:intergenic 25.0%
!! CTTTTCCTTTTTATTCAAGA+AGG + chr4_3:62395370-62395389 Msa0654090:five_prime_UTR 25.0%
! AAGAAATAATTGAGATGGTT+GGG - chr4_3:62395581-62395600 None:intergenic 25.0%
! TAAGAAATAATTGAGATGGT+TGG - chr4_3:62395582-62395601 None:intergenic 25.0%
! AATATGGAAGCTTAATTGAT+TGG + chr4_3:62395613-62395632 Msa0654090:five_prime_UTR 25.0%
! TATAGCTTTCATAAGTCTTA+TGG - chr4_3:62395880-62395899 None:intergenic 25.0%
!!! TAGCTCAAGAGATTTTAAAA+GGG + chr4_3:62396002-62396021 Msa0654090:CDS 25.0%
! TCAATCAATCTTTACATGTT+TGG + chr4_3:62396132-62396151 Msa0654090:intron 25.0%
!! ACCATTAGATATCTTTAAGA+TGG + chr4_3:62396187-62396206 Msa0654090:intron 25.0%
! ACCATCTTAAAGATATCTAA+TGG - chr4_3:62396191-62396210 None:intergenic 25.0%
!! GATGATAATTTTGTAAATGC+AGG - chr4_3:62396269-62396288 None:intergenic 25.0%
! AATTATCATCTGATACGTAT+AGG + chr4_3:62396279-62396298 Msa0654090:intron 25.0%
GAATAACTGTAAGAATGGAT+AGG - chr4_3:62395132-62395151 None:intergenic 30.0%
CTTAGGAATAACTGTAAGAA+TGG - chr4_3:62395137-62395156 None:intergenic 30.0%
TCTGTCTCAAAAAACTTCTT+AGG - chr4_3:62395154-62395173 None:intergenic 30.0%
TTTACAACCTTCTACTGAAA+AGG - chr4_3:62395423-62395442 None:intergenic 30.0%
! TTTTCAGTAGAAGGTTGTAA+AGG + chr4_3:62395422-62395441 Msa0654090:five_prime_UTR 30.0%
AGAAATAATTGAGATGGTTG+GGG - chr4_3:62395580-62395599 None:intergenic 30.0%
TAAGTCAATCACACTTTATC+TGG + chr4_3:62395774-62395793 Msa0654090:intron 30.0%
!!! ACACTTTATCTGGCTTTTAT+TGG + chr4_3:62395784-62395803 Msa0654090:intron 30.0%
! CTAGCTCAAGAGATTTTAAA+AGG + chr4_3:62396001-62396020 Msa0654090:CDS 30.0%
!! AGCTCAAGAGATTTTAAAAG+GGG + chr4_3:62396003-62396022 Msa0654090:CDS 30.0%
! TTGGTAGTAATAAGTCTTTC+TGG + chr4_3:62396065-62396084 Msa0654090:intron 30.0%
! ACATACCACATTTTCTCTTT+AGG + chr4_3:62396090-62396109 Msa0654090:intron 30.0%
! CATACCACATTTTCTCTTTA+GGG + chr4_3:62396091-62396110 Msa0654090:intron 30.0%
GAATAACTGTAAGAATGGAT+AGG - chr4_3:62395132-62395151 None:intergenic 30.0%
CTTAGGAATAACTGTAAGAA+TGG - chr4_3:62395137-62395156 None:intergenic 30.0%
TCTGTCTCAAAAAACTTCTT+AGG - chr4_3:62395154-62395173 None:intergenic 30.0%
TTTACAACCTTCTACTGAAA+AGG - chr4_3:62395423-62395442 None:intergenic 30.0%
! TTTTCAGTAGAAGGTTGTAA+AGG + chr4_3:62395422-62395441 Msa0654090:five_prime_UTR 30.0%
AGAAATAATTGAGATGGTTG+GGG - chr4_3:62395580-62395599 None:intergenic 30.0%
TAAGTCAATCACACTTTATC+TGG + chr4_3:62395774-62395793 Msa0654090:intron 30.0%
!!! ACACTTTATCTGGCTTTTAT+TGG + chr4_3:62395784-62395803 Msa0654090:intron 30.0%
! CTAGCTCAAGAGATTTTAAA+AGG + chr4_3:62396001-62396020 Msa0654090:CDS 30.0%
!! AGCTCAAGAGATTTTAAAAG+GGG + chr4_3:62396003-62396022 Msa0654090:CDS 30.0%
! TTGGTAGTAATAAGTCTTTC+TGG + chr4_3:62396065-62396084 Msa0654090:intron 30.0%
! ACATACCACATTTTCTCTTT+AGG + chr4_3:62396090-62396109 Msa0654090:intron 30.0%
! CATACCACATTTTCTCTTTA+GGG + chr4_3:62396091-62396110 Msa0654090:intron 30.0%
GTAAGCTGTTTATCTGAACA+CGG - chr4_3:62395214-62395233 None:intergenic 35.0%
CGCTACCTTCTTGAATAAAA+AGG - chr4_3:62395378-62395397 None:intergenic 35.0%
CTTATCACAATTGCAAGCAA+AGG - chr4_3:62395526-62395545 None:intergenic 35.0%
CTTTGCTTGCAATTGTGATA+AGG + chr4_3:62395524-62395543 Msa0654090:five_prime_UTR 35.0%
TATGCATCAACAGAGGAATA+TGG - chr4_3:62395726-62395745 None:intergenic 35.0%
CAGATTCATGTAACATCACT+AGG - chr4_3:62395923-62395942 None:intergenic 35.0%
!! TCAAGAGATTTTAAAAGGGG+CGG + chr4_3:62396006-62396025 Msa0654090:CDS 35.0%
AGTTCCCTAAAGAGAAAATG+TGG - chr4_3:62396098-62396117 None:intergenic 35.0%
! TGCAATCATTCGAAAAGCAT+TGG + chr4_3:62396232-62396251 Msa0654090:intron 35.0%
!! GCAATCATTCGAAAAGCATT+GGG + chr4_3:62396233-62396252 Msa0654090:intron 35.0%
ATAATTGGCACATCACTCTT+TGG - chr4_3:62396352-62396371 None:intergenic 35.0%
GTAAGCTGTTTATCTGAACA+CGG - chr4_3:62395214-62395233 None:intergenic 35.0%
CGCTACCTTCTTGAATAAAA+AGG - chr4_3:62395378-62395397 None:intergenic 35.0%
CTTATCACAATTGCAAGCAA+AGG - chr4_3:62395526-62395545 None:intergenic 35.0%
CTTTGCTTGCAATTGTGATA+AGG + chr4_3:62395524-62395543 Msa0654090:five_prime_UTR 35.0%
TATGCATCAACAGAGGAATA+TGG - chr4_3:62395726-62395745 None:intergenic 35.0%
CAGATTCATGTAACATCACT+AGG - chr4_3:62395923-62395942 None:intergenic 35.0%
!! TCAAGAGATTTTAAAAGGGG+CGG + chr4_3:62396006-62396025 Msa0654090:CDS 35.0%
AGTTCCCTAAAGAGAAAATG+TGG - chr4_3:62396098-62396117 None:intergenic 35.0%
! TGCAATCATTCGAAAAGCAT+TGG + chr4_3:62396232-62396251 Msa0654090:intron 35.0%
!! GCAATCATTCGAAAAGCATT+GGG + chr4_3:62396233-62396252 Msa0654090:intron 35.0%
ATAATTGGCACATCACTCTT+TGG - chr4_3:62396352-62396371 None:intergenic 35.0%
CAACCTTCTACTGAAAAGGT+GGG - chr4_3:62395419-62395438 None:intergenic 40.0%
ACAACCTTCTACTGAAAAGG+TGG - chr4_3:62395420-62395439 None:intergenic 40.0%
AGTATGCACTCTTGTCTACA+TGG - chr4_3:62395458-62395477 None:intergenic 40.0%
! TGCATACTTTTGATCGAGTG+TGG + chr4_3:62395470-62395489 Msa0654090:five_prime_UTR 40.0%
!! CATCACCAATGATGGATGAT+AGG - chr4_3:62395497-62395516 None:intergenic 40.0%
TACTAGCAGACAAAGCATGA+TGG - chr4_3:62395670-62395689 None:intergenic 40.0%
ATCAACAGAGGAATATGGTG+TGG - chr4_3:62395721-62395740 None:intergenic 40.0%
TTCCTCTGTTGATGCATAAG+TGG + chr4_3:62395728-62395747 Msa0654090:exon 40.0%
TACCACTTATGCATCAACAG+AGG - chr4_3:62395733-62395752 None:intergenic 40.0%
TCTGTTGATGCATAAGTGGT+AGG + chr4_3:62395732-62395751 Msa0654090:intron 40.0%
AAGGAGTAGAAGCAAAGCTA+TGG + chr4_3:62396038-62396057 Msa0654090:CDS 40.0%
TTTCGAATGATTGCAGAGGA+GGG - chr4_3:62396227-62396246 None:intergenic 40.0%
! TTTTCGAATGATTGCAGAGG+AGG - chr4_3:62396228-62396247 None:intergenic 40.0%
! TGCTTTTCGAATGATTGCAG+AGG - chr4_3:62396231-62396250 None:intergenic 40.0%
AGCGCATCAGGTGTAATAAT+TGG - chr4_3:62396367-62396386 None:intergenic 40.0%
CCATCATTCCAAATCTTGTC+GGG - chr4_3:62396420-62396439 None:intergenic 40.0%
GCCATCATTCCAAATCTTGT+CGG - chr4_3:62396421-62396440 None:intergenic 40.0%
! TGCTCAGTTTAAAGCATTCC+TGG + chr4_3:62396441-62396460 Msa0654090:CDS 40.0%
!!! TTTTACAGCACTGGTTCTCA+AGG + chr4_3:62396520-62396539 Msa0654090:CDS 40.0%
CAACCTTCTACTGAAAAGGT+GGG - chr4_3:62395419-62395438 None:intergenic 40.0%
ACAACCTTCTACTGAAAAGG+TGG - chr4_3:62395420-62395439 None:intergenic 40.0%
AGTATGCACTCTTGTCTACA+TGG - chr4_3:62395458-62395477 None:intergenic 40.0%
! TGCATACTTTTGATCGAGTG+TGG + chr4_3:62395470-62395489 Msa0654090:five_prime_UTR 40.0%
!! CATCACCAATGATGGATGAT+AGG - chr4_3:62395497-62395516 None:intergenic 40.0%
TACTAGCAGACAAAGCATGA+TGG - chr4_3:62395670-62395689 None:intergenic 40.0%
ATCAACAGAGGAATATGGTG+TGG - chr4_3:62395721-62395740 None:intergenic 40.0%
TTCCTCTGTTGATGCATAAG+TGG + chr4_3:62395728-62395747 Msa0654090:exon 40.0%
TACCACTTATGCATCAACAG+AGG - chr4_3:62395733-62395752 None:intergenic 40.0%
TCTGTTGATGCATAAGTGGT+AGG + chr4_3:62395732-62395751 Msa0654090:intron 40.0%
AAGGAGTAGAAGCAAAGCTA+TGG + chr4_3:62396038-62396057 Msa0654090:CDS 40.0%
TTTCGAATGATTGCAGAGGA+GGG - chr4_3:62396227-62396246 None:intergenic 40.0%
! TTTTCGAATGATTGCAGAGG+AGG - chr4_3:62396228-62396247 None:intergenic 40.0%
! TGCTTTTCGAATGATTGCAG+AGG - chr4_3:62396231-62396250 None:intergenic 40.0%
AGCGCATCAGGTGTAATAAT+TGG - chr4_3:62396367-62396386 None:intergenic 40.0%
CCATCATTCCAAATCTTGTC+GGG - chr4_3:62396420-62396439 None:intergenic 40.0%
GCCATCATTCCAAATCTTGT+CGG - chr4_3:62396421-62396440 None:intergenic 40.0%
! TGCTCAGTTTAAAGCATTCC+TGG + chr4_3:62396441-62396460 Msa0654090:CDS 40.0%
!!! TTTTACAGCACTGGTTCTCA+AGG + chr4_3:62396520-62396539 Msa0654090:CDS 40.0%
! AGACCCACCTTTTCAGTAGA+AGG + chr4_3:62395413-62395432 Msa0654090:five_prime_UTR 45.0%
GTGGTCCTATCATCCATCAT+TGG + chr4_3:62395489-62395508 Msa0654090:five_prime_UTR 45.0%
GGAAGAGTCATCACCAATGA+TGG - chr4_3:62395505-62395524 None:intergenic 45.0%
CTTTGCTTCTACTCCTTCCA+CGG - chr4_3:62396035-62396054 None:intergenic 45.0%
AGTAGAAGCAAAGCTATGGC+AGG + chr4_3:62396042-62396061 Msa0654090:CDS 45.0%
! TTGCAGAGGAGGGTATAACT+TGG - chr4_3:62396217-62396236 None:intergenic 45.0%
ATTGGCACATCACTCTTTGG+AGG - chr4_3:62396349-62396368 None:intergenic 45.0%
! CCCGACAAGATTTGGAATGA+TGG + chr4_3:62396417-62396436 Msa0654090:CDS 45.0%
! AAAGCATTCCTGGATGCAAC+TGG + chr4_3:62396451-62396470 Msa0654090:CDS 45.0%
GTGCTGTAAAAGATTCCTGC+AGG - chr4_3:62396511-62396530 None:intergenic 45.0%
! GCAGGAATCTTTTACAGCAC+TGG + chr4_3:62396511-62396530 Msa0654090:CDS 45.0%
! AGACCCACCTTTTCAGTAGA+AGG + chr4_3:62395413-62395432 Msa0654090:five_prime_UTR 45.0%
GTGGTCCTATCATCCATCAT+TGG + chr4_3:62395489-62395508 Msa0654090:five_prime_UTR 45.0%
GGAAGAGTCATCACCAATGA+TGG - chr4_3:62395505-62395524 None:intergenic 45.0%
CTTTGCTTCTACTCCTTCCA+CGG - chr4_3:62396035-62396054 None:intergenic 45.0%
AGTAGAAGCAAAGCTATGGC+AGG + chr4_3:62396042-62396061 Msa0654090:CDS 45.0%
! TTGCAGAGGAGGGTATAACT+TGG - chr4_3:62396217-62396236 None:intergenic 45.0%
ATTGGCACATCACTCTTTGG+AGG - chr4_3:62396349-62396368 None:intergenic 45.0%
! CCCGACAAGATTTGGAATGA+TGG + chr4_3:62396417-62396436 Msa0654090:CDS 45.0%
! AAAGCATTCCTGGATGCAAC+TGG + chr4_3:62396451-62396470 Msa0654090:CDS 45.0%
GTGCTGTAAAAGATTCCTGC+AGG - chr4_3:62396511-62396530 None:intergenic 45.0%
! GCAGGAATCTTTTACAGCAC+TGG + chr4_3:62396511-62396530 Msa0654090:CDS 45.0%
GAAGCAAAGCTATGGCAGGT+TGG + chr4_3:62396046-62396065 Msa0654090:intron 50.0%
AGCAACAAACCATCAGCCTC+AGG - chr4_3:62396391-62396410 None:intergenic 50.0%
!! GAGGCTGATGGTTTGTTGCT+TGG + chr4_3:62396391-62396410 Msa0654090:CDS 50.0%
CTTGGCTTCCCGACAAGATT+TGG + chr4_3:62396409-62396428 Msa0654090:CDS 50.0%
!! TACAGCACTGGTTCTCAAGG+AGG + chr4_3:62396523-62396542 Msa0654090:CDS 50.0%
GAAGCAAAGCTATGGCAGGT+TGG + chr4_3:62396046-62396065 Msa0654090:intron 50.0%
AGCAACAAACCATCAGCCTC+AGG - chr4_3:62396391-62396410 None:intergenic 50.0%
!! GAGGCTGATGGTTTGTTGCT+TGG + chr4_3:62396391-62396410 Msa0654090:CDS 50.0%
CTTGGCTTCCCGACAAGATT+TGG + chr4_3:62396409-62396428 Msa0654090:CDS 50.0%
!! TACAGCACTGGTTCTCAAGG+AGG + chr4_3:62396523-62396542 Msa0654090:CDS 50.0%
TGCTTCTACTCCTTCCACGG+AGG - chr4_3:62396032-62396051 None:intergenic 55.0%
GGTACCTGAAACTCTGCCTG+CGG + chr4_3:62396300-62396319 Msa0654090:intron 55.0%
TACACCTGATGCGCTACCTG+AGG + chr4_3:62396372-62396391 Msa0654090:CDS 55.0%
GCATTCCTGGATGCAACTGG+TGG + chr4_3:62396454-62396473 Msa0654090:CDS 55.0%
ATAGGCCACCAGTTGCATCC+AGG - chr4_3:62396462-62396481 None:intergenic 55.0%
TGGATGCAACTGGTGGCCTA+TGG + chr4_3:62396461-62396480 Msa0654090:CDS 55.0%
CAAGTGCCTGAGTACGCCAT+AGG - chr4_3:62396480-62396499 None:intergenic 55.0%
!! AGCACTGGTTCTCAAGGAGG+AGG + chr4_3:62396526-62396545 Msa0654090:CDS 55.0%
TGCTTCTACTCCTTCCACGG+AGG - chr4_3:62396032-62396051 None:intergenic 55.0%
GGTACCTGAAACTCTGCCTG+CGG + chr4_3:62396300-62396319 Msa0654090:intron 55.0%
TACACCTGATGCGCTACCTG+AGG + chr4_3:62396372-62396391 Msa0654090:CDS 55.0%
GCATTCCTGGATGCAACTGG+TGG + chr4_3:62396454-62396473 Msa0654090:CDS 55.0%
ATAGGCCACCAGTTGCATCC+AGG - chr4_3:62396462-62396481 None:intergenic 55.0%
TGGATGCAACTGGTGGCCTA+TGG + chr4_3:62396461-62396480 Msa0654090:CDS 55.0%
CAAGTGCCTGAGTACGCCAT+AGG - chr4_3:62396480-62396499 None:intergenic 55.0%
!! AGCACTGGTTCTCAAGGAGG+AGG + chr4_3:62396526-62396545 Msa0654090:CDS 55.0%
TTTAAAAGGGGCGGCCTCCG+TGG + chr4_3:62396015-62396034 Msa0654090:CDS 60.0%
ACCTGAAACTCTGCCTGCGG+AGG + chr4_3:62396303-62396322 Msa0654090:CDS 60.0%
ACCTCCGCAGGCAGAGTTTC+AGG - chr4_3:62396307-62396326 None:intergenic 60.0%
ATCTTCCCAACGACCTCCGC+AGG - chr4_3:62396319-62396338 None:intergenic 60.0%
TGCGGAGGTCGTTGGGAAGA+TGG + chr4_3:62396318-62396337 Msa0654090:CDS 60.0%
GCGGAGGTCGTTGGGAAGAT+GGG + chr4_3:62396319-62396338 Msa0654090:CDS 60.0%
GAGGTCGTTGGGAAGATGGG+AGG + chr4_3:62396322-62396341 Msa0654090:CDS 60.0%
TCAGCCTCAGGTAGCGCATC+AGG - chr4_3:62396379-62396398 None:intergenic 60.0%
GATGCGCTACCTGAGGCTGA+TGG + chr4_3:62396379-62396398 Msa0654090:CDS 60.0%
TGGTGGCCTATGGCGTACTC+AGG + chr4_3:62396471-62396490 Msa0654090:CDS 60.0%
TGGCGTACTCAGGCACTTGC+AGG + chr4_3:62396481-62396500 Msa0654090:CDS 60.0%
GCACTTGCAGGAAAGCCTGC+AGG + chr4_3:62396493-62396512 Msa0654090:CDS 60.0%
TTTAAAAGGGGCGGCCTCCG+TGG + chr4_3:62396015-62396034 Msa0654090:CDS 60.0%
ACCTGAAACTCTGCCTGCGG+AGG + chr4_3:62396303-62396322 Msa0654090:CDS 60.0%
ACCTCCGCAGGCAGAGTTTC+AGG - chr4_3:62396307-62396326 None:intergenic 60.0%
ATCTTCCCAACGACCTCCGC+AGG - chr4_3:62396319-62396338 None:intergenic 60.0%
TGCGGAGGTCGTTGGGAAGA+TGG + chr4_3:62396318-62396337 Msa0654090:CDS 60.0%
GCGGAGGTCGTTGGGAAGAT+GGG + chr4_3:62396319-62396338 Msa0654090:CDS 60.0%
GAGGTCGTTGGGAAGATGGG+AGG + chr4_3:62396322-62396341 Msa0654090:CDS 60.0%
TCAGCCTCAGGTAGCGCATC+AGG - chr4_3:62396379-62396398 None:intergenic 60.0%
GATGCGCTACCTGAGGCTGA+TGG + chr4_3:62396379-62396398 Msa0654090:CDS 60.0%
TGGTGGCCTATGGCGTACTC+AGG + chr4_3:62396471-62396490 Msa0654090:CDS 60.0%
TGGCGTACTCAGGCACTTGC+AGG + chr4_3:62396481-62396500 Msa0654090:CDS 60.0%
GCACTTGCAGGAAAGCCTGC+AGG + chr4_3:62396493-62396512 Msa0654090:CDS 60.0%
ACTCTGCCTGCGGAGGTCGT+TGG + chr4_3:62396310-62396329 Msa0654090:CDS 65.0%
CTCTGCCTGCGGAGGTCGTT+GGG + chr4_3:62396311-62396330 Msa0654090:CDS 65.0%
ACTCTGCCTGCGGAGGTCGT+TGG + chr4_3:62396310-62396329 Msa0654090:CDS 65.0%
CTCTGCCTGCGGAGGTCGTT+GGG + chr4_3:62396311-62396330 Msa0654090:CDS 65.0%
AAAGGGGCGGCCTCCGTGGA+AGG + chr4_3:62396019-62396038 Msa0654090:CDS 70.0%
AAAGGGGCGGCCTCCGTGGA+AGG + chr4_3:62396019-62396038 Msa0654090:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 62394981 62396567 62394981 ID=Msa0654090;Name=Msa0654090
chr4_3 mRNA 62394981 62396567 62394981 ID=Msa0654090-mRNA-1;Parent=Msa0654090;Name=Msa0654090-mRNA-1;_AED=0.29;_eAED=0.29;_QI=758|1|0.66|1|0.5|0.66|3|0|123
chr4_3 exon 62394981 62395749 62394981 ID=Msa0654090-mRNA-1:exon:17303;Parent=Msa0654090-mRNA-1
chr4_3 exon 62395970 62396063 62395970 ID=Msa0654090-mRNA-1:exon:17304;Parent=Msa0654090-mRNA-1
chr4_3 exon 62396301 62396567 62396301 ID=Msa0654090-mRNA-1:exon:17305;Parent=Msa0654090-mRNA-1
chr4_3 five_prime_UTR 62394981 62395738 62394981 ID=Msa0654090-mRNA-1:five_prime_utr;Parent=Msa0654090-mRNA-1
chr4_3 CDS 62395739 62395749 62395739 ID=Msa0654090-mRNA-1:cds;Parent=Msa0654090-mRNA-1
chr4_3 CDS 62395970 62396063 62395970 ID=Msa0654090-mRNA-1:cds;Parent=Msa0654090-mRNA-1
chr4_3 CDS 62396301 62396567 62396301 ID=Msa0654090-mRNA-1:cds;Parent=Msa0654090-mRNA-1
Gene Sequence

>Msa0654090

ATGCATAAGTGTTACTATTCTATGTACGGACATGTTGAGAAGCTAGCTCAAGAGATTTTAAAAGGGGCGGCCTCCGTGGAAGGAGTAGAAGCAAAGCTATGGCAGGTACCTGAAACTCTGCCTGCGGAGGTCGTTGGGAAGATGGGAGGACCTCCAAAGAGTGATGTGCCAATTATTACACCTGATGCGCTACCTGAGGCTGATGGTTTGTTGCTTGGCTTCCCGACAAGATTTGGAATGATGGCTGCTCAGTTTAAAGCATTCCTGGATGCAACTGGTGGCCTATGGCGTACTCAGGCACTTGCAGGAAAGCCTGCAGGAATCTTTTACAGCACTGGTTCTCAAGGAGGAGGACAAGAGACTACACCGTAA
Protein sequence

>Msa0654090

MHKCYYSMYGHVEKLAQEILKGAASVEGVEAKLWQVPETLPAEVVGKMGGPPKSDVPIITPDALPEADGLLLGFPTRFGMMAAQFKAFLDATGGLWRTQALAGKPAGIFYSTGSQGGGQETTP*