AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0654290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0654290 MtrunA17_Chr4g0048911 81.723 476 26 2 1 426 1 465 0.0 795
Msa0654290 MtrunA17_Chr3g0109031 44.175 412 173 5 74 428 13 424 7.56e-120 359
Msa0654290 MtrunA17_Chr6g0477111 44.361 399 164 6 87 428 2 399 2.10e-116 349
Msa0654290 MtrunA17_Chr3g0109021 43.204 412 177 5 74 428 13 424 4.86e-113 341
Msa0654290 MtrunA17_Chr3g0108941 43.584 413 175 6 74 428 13 425 3.81e-112 339
Msa0654290 MtrunA17_Chr3g0109001 42.488 426 186 6 60 428 2 425 4.07e-112 339
Msa0654290 MtrunA17_Chr3g0105691 42.718 412 179 5 74 428 46 457 4.09e-112 340
Msa0654290 MtrunA17_Chr3g0108981 44.361 399 165 5 87 428 2 400 4.73e-112 338
Msa0654290 MtrunA17_Chr3g0108961 44.361 399 165 5 87 428 2 400 4.73e-112 338
Msa0654290 MtrunA17_Chr3g0109011 43.970 398 167 4 87 428 2 399 5.76e-112 338
Msa0654290 MtrunA17_Chr3g0099031 42.824 425 185 5 60 428 2 424 1.25e-111 338
Msa0654290 MtrunA17_Chr3g0108991 42.169 415 183 5 74 431 13 427 3.81e-111 337
Msa0654290 MtrunA17_Chr3g0108951 42.169 415 183 5 74 431 13 427 3.81e-111 337
Msa0654290 MtrunA17_Chr3g0108971 43.108 399 171 4 87 429 2 400 2.45e-110 333
Msa0654290 MtrunA17_Chr2g0280581 41.232 422 190 5 63 426 5 426 3.15e-110 334
Msa0654290 MtrunA17_Chr4g0048921 43.521 409 175 7 66 422 65 469 2.81e-104 320
Msa0654290 MtrunA17_Chr6g0477121 45.808 334 123 6 152 428 10 342 4.58e-98 300
Msa0654290 MtrunA17_Chr2g0323151 35.751 386 188 4 89 422 4 381 1.52e-76 246
Msa0654290 MtrunA17_Chr2g0323131 32.080 452 236 7 27 422 30 466 4.08e-76 247
Msa0654290 MtrunA17_Chr4g0042111 32.187 407 218 7 71 422 58 461 5.86e-63 213
Msa0654290 MtrunA17_Chr2g0310331 32.304 421 226 6 55 422 72 486 2.06e-58 201
Msa0654290 MtrunA17_Chr6g0451711 26.905 420 244 6 72 433 55 469 6.70e-55 191
Msa0654290 MtrunA17_Chr8g0357411 29.216 421 238 6 55 421 74 488 5.04e-54 189
Msa0654290 MtrunA17_Chr6g0465441 28.506 435 243 9 61 433 37 465 2.29e-53 187
Msa0654290 MtrunA17_Chr6g0465851 28.079 406 227 7 87 433 31 430 9.68e-53 184
Msa0654290 MtrunA17_Chr6g0465911 28.079 406 227 7 87 433 66 465 1.65e-52 184
Msa0654290 MtrunA17_Chr5g0425371 27.578 417 237 6 78 433 53 465 2.70e-52 184
Msa0654290 MtrunA17_Chr6g0466051 27.833 406 228 7 87 433 66 465 7.55e-52 182
Msa0654290 MtrunA17_Chr7g0236991 27.040 429 245 7 67 433 43 465 1.97e-50 179
Msa0654290 MtrunA17_Chr7g0237001 27.040 429 245 7 67 433 43 465 2.21e-50 179
Msa0654290 MtrunA17_Chr7g0237041 27.040 429 245 7 67 433 43 465 2.21e-50 179
Msa0654290 MtrunA17_Chr7g0237081 27.040 429 245 7 67 433 43 465 2.21e-50 179
Msa0654290 MtrunA17_Chr7g0237051 27.040 429 245 7 67 433 43 465 2.21e-50 179
Msa0654290 MtrunA17_Chr7g0237011 26.799 403 232 5 89 433 13 410 2.41e-50 177
Msa0654290 MtrunA17_Chr7g0236961 26.799 403 232 5 89 433 13 410 2.78e-50 177
Msa0654290 MtrunA17_Chr2g0319041 27.907 430 250 10 60 432 40 466 3.98e-50 178
Msa0654290 MtrunA17_Chr8g0336181 28.846 416 229 7 71 426 51 459 6.02e-50 177
Msa0654290 MtrunA17_Chr4g0024691 27.792 403 237 4 72 422 60 460 1.34e-49 177
Msa0654290 MtrunA17_Chr5g0431651 27.612 402 228 5 78 422 56 451 5.56e-49 175
Msa0654290 MtrunA17_Chr4g0023911 43.719 199 111 1 225 422 282 480 6.61e-49 175
Msa0654290 MtrunA17_Chr4g0023911 38.571 140 83 2 65 203 71 208 9.80e-22 97.8
Msa0654290 MtrunA17_Chr3g0105071 27.763 389 219 5 91 422 73 456 1.02e-48 174
Msa0654290 MtrunA17_Chr4g0008621 28.608 395 217 9 49 384 37 425 1.27e-48 171
Msa0654290 MtrunA17_Chr2g0319051 28.710 411 235 9 71 426 10 417 4.23e-48 172
Msa0654290 MtrunA17_Chr2g0310401 32.927 328 166 3 149 422 5 332 7.78e-48 169
Msa0654290 MtrunA17_Chr5g0431571 28.019 414 233 6 71 426 46 452 5.91e-46 166
Msa0654290 MtrunA17_Chr3g0082691 27.206 408 235 8 78 426 52 456 3.14e-44 162
Msa0654290 MtrunA17_Chr7g0237031 29.553 291 144 4 89 323 13 298 3.68e-39 143
Msa0654290 MtrunA17_Chr7g0236951 29.553 291 144 4 89 323 13 298 5.33e-39 142
Msa0654290 MtrunA17_Chr7g0217361 37.681 207 112 5 225 422 11 209 1.75e-37 138
Msa0654290 MtrunA17_Chr8g0357231 40.881 159 91 2 265 421 1 158 4.19e-36 133
Msa0654290 MtrunA17_Chr6g0465931 30.272 294 142 5 87 323 31 318 4.72e-36 135
Msa0654290 MtrunA17_Chr6g0466111 30.272 294 142 5 87 323 31 318 6.03e-36 135
Msa0654290 MtrunA17_Chr6g0465981 30.272 294 142 5 87 323 66 353 1.18e-35 135
Msa0654290 MtrunA17_Chr6g0465871 30.272 294 142 5 87 323 66 353 1.18e-35 135
Msa0654290 MtrunA17_Chr6g0466081 30.272 294 142 5 87 323 66 353 1.18e-35 135
Msa0654290 MtrunA17_Chr7g0236971 29.858 211 146 1 225 433 103 313 5.88e-32 125
Msa0654290 MtrunA17_Chr2g0319081 29.524 210 146 2 224 432 139 347 2.70e-29 118
Msa0654290 MtrunA17_Chr7g0237061 29.412 170 116 1 265 434 2 167 3.95e-23 97.4
Msa0654290 MtrunA17_Chr6g0466031 29.070 172 118 3 265 433 2 172 1.19e-22 96.7
Msa0654290 MtrunA17_Chr5g0445511 26.496 234 119 4 171 352 2 234 9.76e-22 93.6
Msa0654290 MtrunA17_Chr6g0466021 36.170 141 80 3 87 223 66 200 5.22e-20 87.8
Msa0654290 MtrunA17_Chr4g0050281 61.194 67 24 1 7 71 37 103 1.56e-18 81.3
Msa0654290 MtrunA17_Chr7g0237071 33.871 124 73 2 89 208 13 131 1.26e-17 81.6
Msa0654290 MtrunA17_Chr2g0310121 27.225 191 137 2 235 424 492 681 6.29e-17 83.6
Msa0654290 MtrunA17_Chr3g0101681 26.316 190 138 2 235 423 493 681 2.22e-16 81.6
Msa0654290 MtrunA17_Chr2g0319071 38.333 120 68 1 89 208 46 159 2.31e-16 81.3
Msa0654290 MtrunA17_Chr2g0319071 28.462 130 91 2 298 426 264 392 1.76e-13 72.0
Msa0654290 MtrunA17_Chr2g0281651 26.733 202 141 5 225 424 68 264 5.16e-15 76.6
Msa0654290 MtrunA17_Chr8g0357221 28.402 169 115 3 55 223 74 236 1.17e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0654290 AT1G61680.1 37.787 479 224 7 8 426 17 481 9.70e-103 317
Msa0654290 AT1G61680.2 37.161 479 220 8 8 426 17 474 1.45e-98 306
Msa0654290 AT4G16740.1 30.905 398 203 12 87 422 76 463 3.16e-48 173
Msa0654290 AT4G16730.2 38.384 198 122 0 225 422 192 389 6.58e-44 160
Msa0654290 AT4G16730.2 37.374 99 59 2 91 189 1 96 2.54e-13 72.0
Msa0654290 AT4G16730.1 38.384 198 122 0 225 422 238 435 1.31e-43 160
Msa0654290 AT4G16730.1 32.593 135 82 3 76 204 26 157 2.46e-14 75.5
Msa0654290 AT2G24210.1 28.733 442 243 13 56 433 66 499 4.95e-42 157
Msa0654290 AT5G23960.2 26.812 414 242 7 65 422 37 445 1.95e-40 152
Msa0654290 AT5G23960.1 26.683 416 242 8 65 422 37 447 5.36e-39 148
Msa0654290 AT3G25820.1 28.928 401 221 11 82 422 93 489 6.24e-39 148
Msa0654290 AT3G25830.1 28.928 401 221 11 82 422 93 489 6.24e-39 148
Msa0654290 AT3G25810.1 35.922 206 129 2 219 422 287 491 4.36e-36 140
Msa0654290 AT1G33750.1 23.587 407 245 5 78 424 104 504 5.55e-32 128
Msa0654290 AT3G29410.2 25.381 394 229 9 87 422 11 397 7.56e-29 118
Msa0654290 AT3G29410.1 25.000 416 240 10 72 422 94 502 8.41e-29 119
Msa0654290 AT4G16740.2 29.412 323 160 10 87 348 76 391 1.57e-28 116
Msa0654290 AT5G48110.1 35.678 199 125 3 225 422 44 240 6.83e-28 113
Msa0654290 AT1G70080.1 32.338 201 131 3 225 422 311 509 6.36e-27 114
Msa0654290 AT3G25820.2 25.799 407 232 11 14 361 32 427 1.62e-26 111
Msa0654290 AT3G14490.1 22.418 397 247 6 83 422 107 499 1.61e-25 109
Msa0654290 AT4G13300.1 24.227 388 234 7 91 422 69 452 2.98e-25 108
Msa0654290 AT5G44630.1 24.688 401 238 9 79 422 63 456 1.09e-23 103
Msa0654290 AT4G20210.1 24.060 399 242 7 83 424 107 501 1.15e-21 98.2
Msa0654290 AT1G79460.1 29.534 193 134 2 233 424 488 679 1.03e-20 95.5
Msa0654290 AT4G20230.2 22.977 383 236 6 98 424 138 517 1.65e-20 94.7
Msa0654290 AT4G20230.1 22.977 383 236 6 98 424 126 505 1.99e-20 94.4
Msa0654290 AT2G23230.2 23.415 410 252 12 83 433 105 511 1.08e-19 92.0
Msa0654290 AT1G31950.3 25.000 204 141 3 225 422 308 505 1.16e-19 92.0
Msa0654290 AT1G31950.1 25.000 204 141 3 225 422 307 504 1.17e-19 92.0
Msa0654290 AT1G31950.2 25.000 204 141 3 225 422 308 505 1.48e-19 91.3
Msa0654290 AT3G14520.1 23.232 198 152 0 225 422 305 502 1.91e-19 91.3
Msa0654290 AT3G14520.1 25.352 142 103 2 65 206 92 230 1.09e-11 67.4
Msa0654290 AT3G29110.1 24.872 390 234 9 91 424 112 498 2.33e-19 90.9
Msa0654290 AT4G15870.1 29.353 201 129 3 225 424 310 498 1.02e-18 89.0
Msa0654290 AT3G14540.2 24.623 199 148 2 225 422 302 499 1.23e-18 88.6
Msa0654290 AT4G16740.4 28.676 272 127 9 87 297 76 341 1.27e-18 87.4
Msa0654290 AT4G16740.3 28.676 272 127 9 87 297 76 341 1.27e-18 87.4
Msa0654290 AT3G14540.1 24.623 199 148 2 225 422 302 499 1.27e-18 89.0
Msa0654290 AT2G23230.1 23.844 411 244 13 83 433 105 506 1.82e-18 88.2
Msa0654290 AT3G32030.1 23.232 198 152 0 225 422 305 502 1.28e-16 82.8
Msa0654290 AT3G29190.1 26.961 204 147 2 225 426 301 504 3.03e-16 81.6
Msa0654290 AT3G25820.4 27.007 274 139 9 82 297 93 363 4.15e-16 80.1
Msa0654290 AT3G25820.3 27.007 274 139 9 82 297 93 363 4.15e-16 80.1
Msa0654290 AT4G20200.1 24.752 202 150 2 225 424 304 505 2.91e-15 78.6
Msa0654290 AT4G13280.2 28.000 200 141 2 225 422 254 452 6.02e-15 77.4
Msa0654290 AT1G48800.1 23.786 412 232 14 83 426 112 509 1.38e-14 76.3
Msa0654290 AT1G61120.1 27.230 213 147 4 215 424 477 684 2.76e-13 72.4
Msa0654290 AT1G66020.1 27.228 202 145 2 225 424 298 499 2.92e-13 72.0
Msa0654290 AT1G48820.1 21.615 384 201 10 98 424 124 464 4.17e-13 71.6

Find 145 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATACTCCTCTGAATT+TGG 0.087434 4_3:+62473243 None:intergenic
AAACAAAAGCACAAATATTT+TGG 0.114515 4_3:-62475763 Msa0654290:CDS
ATCACATAGCCTTAGAATTT+TGG 0.127459 4_3:+62473095 None:intergenic
TATCATCAATGATGTATATT+AGG 0.172952 4_3:+62473711 None:intergenic
ATACTCCTTGTTTGGTTGTT+TGG 0.215702 4_3:-62471913 Msa0654290:three_prime_UTR
CAAGGTGTGTGCCACAAATT+TGG 0.217226 4_3:+62474536 None:intergenic
TTAATTCTACTTTAAAATAT+TGG 0.218641 4_3:-62473022 Msa0654290:three_prime_UTR
TACTCCTTGTTTGGTTGTTT+GGG 0.231065 4_3:-62471912 Msa0654290:three_prime_UTR
CCATCATAATTGTACATTAT+AGG 0.232144 4_3:+62472193 None:intergenic
TGAGGATATAAATGGATTAA+TGG 0.239175 4_3:-62474676 Msa0654290:CDS
GTGGTCCAATATTTATTCTT+TGG 0.249478 4_3:+62472963 None:intergenic
ATTCAAGACAGTAGTTAAAT+TGG 0.250833 4_3:+62475695 None:intergenic
GACCACGAGTACCATTTAAT+TGG 0.252942 4_3:+62473797 None:intergenic
TTGTTTGGGAGCTGGGAATT+AGG 0.275658 4_3:-62471898 Msa0654290:three_prime_UTR
CTTTCTTGCAAGAAGCAAAA+TGG 0.287959 4_3:-62473284 Msa0654290:CDS
GTCCTTGATATTCATTTCTC+TGG 0.295905 4_3:+62475340 None:intergenic
TTGTTTGGTTGTTTGGGAGC+TGG 0.298477 4_3:-62471906 Msa0654290:three_prime_UTR
CTAGAGATGAACCAATTAAA+TGG 0.303622 4_3:-62473808 Msa0654290:CDS
AGACAACACTATTGGTCTAT+TGG 0.313372 4_3:+62472704 None:intergenic
GAAACTAGAATAGGCATGTT+TGG 0.319225 4_3:+62475728 None:intergenic
TAACCAATGAATTTGCTCTT+AGG 0.320272 4_3:-62473474 Msa0654290:CDS
GAAGCAAAATGGTTTGGTTC+TGG 0.325374 4_3:-62473273 Msa0654290:CDS
CTTAGGTTTGGCAAAGGACT+TGG 0.338530 4_3:-62473837 Msa0654290:CDS
TTTGGCTGTTGTAGATATAA+TGG 0.338929 4_3:+62473113 None:intergenic
AAATCCAAAGCTTATTGTAT+TGG 0.348577 4_3:-62475889 Msa0654290:five_prime_UTR
CTACAACAGCCAAAATTCTA+AGG 0.352122 4_3:-62473104 Msa0654290:CDS
ATCATCAATGATGTATATTA+GGG 0.352665 4_3:+62473712 None:intergenic
TGAGAAAACCGTGTCTCTAA+TGG 0.359133 4_3:-62473148 Msa0654290:CDS
TACAGACGAATGTTTATCTA+TGG 0.359859 4_3:-62475460 Msa0654290:CDS
GATAACCCAGGAGTTTCTAT+TGG 0.360210 4_3:-62472343 Msa0654290:three_prime_UTR
TTTAACCTCATTAATAAAAT+CGG 0.373362 4_3:-62473425 Msa0654290:intron
TGTTTGGTTGTTTGGGAGCT+GGG 0.377861 4_3:-62471905 Msa0654290:three_prime_UTR
ATGACCAGGCATTAAGATAT+TGG 0.379692 4_3:+62474583 None:intergenic
GCTTCTCAATTGAGCATAGA+AGG 0.389737 4_3:-62474642 Msa0654290:CDS
TGTGAGCTCAATCCTTTCTT+CGG 0.393162 4_3:+62473745 None:intergenic
TGAAGAAAATGTTGAATGAT+TGG 0.395622 4_3:+62475785 None:intergenic
ATGTGCCAAAGAATAAATAT+TGG 0.395885 4_3:-62472968 Msa0654290:three_prime_UTR
TAGTGTTGTCTCAGATGATT+AGG 0.397016 4_3:-62472692 Msa0654290:three_prime_UTR
TTGCAAGAAGCAAAATGGTT+TGG 0.397800 4_3:-62473279 Msa0654290:CDS
ATAGTTCATGTATGTATTGC+GGG 0.400671 4_3:+62472912 None:intergenic
GGCACATTACTAATAGTCTA+TGG 0.401321 4_3:-62475835 Msa0654290:exon
GAAACCAATACAATAAGCTT+TGG 0.402507 4_3:+62475885 None:intergenic
TCTTCGGAGAACTGTGGACC+TGG 0.403166 4_3:+62473761 None:intergenic
GCATAGTAAGAATTATTACT+AGG 0.406892 4_3:-62472590 Msa0654290:three_prime_UTR
CTGCAGAATGCTTTGATGTT+GGG 0.412622 4_3:+62472095 None:intergenic
GTTTGTCCAATAGAAACTCC+TGG 0.424695 4_3:+62472337 None:intergenic
GTTAATTGTGAGGGTAATGA+TGG 0.436983 4_3:-62472391 Msa0654290:three_prime_UTR
AAACTAGAATAGGCATGTTT+GGG 0.438056 4_3:+62475729 None:intergenic
GTTTGGACGCAGATGGGACT+TGG 0.438150 4_3:-62473554 Msa0654290:intron
GTGCACTCCAGTGCTTACTA+TGG 0.439132 4_3:+62473215 None:intergenic
TTAGTAAAAGATGATGGTAA+TGG 0.444587 4_3:+62472244 None:intergenic
ATCATCTGAGACAACACTAT+TGG 0.447386 4_3:+62472696 None:intergenic
TTTATTATAGAAACTAGAAT+AGG 0.448429 4_3:+62475719 None:intergenic
CAAATTCATACTCCTAAAAG+TGG 0.456424 4_3:-62475672 Msa0654290:CDS
GCCTGGTCATCAACATTTCA+TGG 0.462096 4_3:-62474570 Msa0654290:CDS
GTGTGGATATACTCCTTGTT+TGG 0.468711 4_3:-62471921 Msa0654290:three_prime_UTR
CTGCATGAAAGTGTGTTTCA+AGG 0.472196 4_3:-62473509 Msa0654290:CDS
ATTAATGAAGTTAGAAACAT+AGG 0.477000 4_3:+62473040 None:intergenic
TATAGATGGTGGAAAGACTT+AGG 0.478515 4_3:-62473854 Msa0654290:intron
GACTCGGGTTCAGTAAAATC+AGG 0.478690 4_3:+62471951 None:intergenic
TCAAACAAATCATGTATTGC+GGG 0.491332 4_3:-62472500 Msa0654290:three_prime_UTR
GTGAAATCGTTGCTTTATGA+TGG 0.495348 4_3:-62472142 Msa0654290:three_prime_UTR
AAACACACTTTCATGCAGTC+TGG 0.496127 4_3:+62473514 None:intergenic
ATGGTGGAAAGACTTAGGTT+TGG 0.496137 4_3:-62473849 Msa0654290:CDS
GTGAACAAATTTCAGATGCA+TGG 0.498506 4_3:-62472300 Msa0654290:three_prime_UTR
TGGTCAATGGCATGTCTCCC+AGG 0.498526 4_3:-62473779 Msa0654290:CDS
TCTAAGGCTATGTGATGACT+TGG 0.498674 4_3:-62473088 Msa0654290:CDS
CATAGTAAGAATTATTACTA+GGG 0.499741 4_3:-62472589 Msa0654290:three_prime_UTR
TGTCCTAAGAGCAAATTCAT+TGG 0.505116 4_3:+62473471 None:intergenic
GTCCACAGTTCTCCGAAGAA+AGG 0.506139 4_3:-62473757 Msa0654290:CDS
AGCATTGGATGAACTTAAGC+AGG 0.506378 4_3:-62475508 Msa0654290:CDS
TATAAGACATGCAAAAGCAT+TGG 0.509430 4_3:-62475523 Msa0654290:CDS
TTTCGTATGTTGAGACAAGA+AGG 0.509440 4_3:-62475297 Msa0654290:CDS
CCTATAATGTACAATTATGA+TGG 0.511514 4_3:-62472193 Msa0654290:three_prime_UTR
AGAATAGCATAGACACACTT+TGG 0.511989 4_3:-62471874 Msa0654290:three_prime_UTR
CCATCCTCAAGCCAAATTTG+TGG 0.515915 4_3:-62474547 Msa0654290:CDS
TTCCAGAGAAATGAATATCA+AGG 0.522241 4_3:-62475342 Msa0654290:CDS
ACTGCAGAATGCTTTGATGT+TGG 0.532928 4_3:+62472094 None:intergenic
AGACTATTAGTAATGTGCCT+TGG 0.534516 4_3:+62475839 None:intergenic
GTGGATTCCATCAAAAGATT+AGG 0.535767 4_3:-62475438 Msa0654290:CDS
TTTGTCCAATAGAAACTCCT+GGG 0.537264 4_3:+62472338 None:intergenic
TATCCACACTTTATGTGACT+CGG 0.537819 4_3:+62471935 None:intergenic
AGACGAATGTTTATCTATGG+TGG 0.538797 4_3:-62475457 Msa0654290:CDS
ATCATGTATTGCGGGACGGA+GGG 0.538896 4_3:-62472492 Msa0654290:three_prime_UTR
GCCACCAATATCTTAATGCC+TGG 0.539711 4_3:-62474587 Msa0654290:CDS
GCTGTTGTAGATATAATGGT+TGG 0.540737 4_3:+62473117 None:intergenic
TAAATGGTACTCGTGGTCAA+TGG 0.541293 4_3:-62473792 Msa0654290:CDS
CAAAGTTTAGTAAAAGATGA+TGG 0.541713 4_3:+62472238 None:intergenic
AAGAATGCCATAGTAAGCAC+TGG 0.543981 4_3:-62473222 Msa0654290:CDS
TTCCATCAAAAGATTAGGCA+TGG 0.555371 4_3:-62475433 Msa0654290:CDS
GCATAGACACACTTTGGAAG+AGG 0.555805 4_3:-62471868 Msa0654290:three_prime_UTR
TGCAGAATGCTTTGATGTTG+GGG 0.557340 4_3:+62472096 None:intergenic
ATCCACACTTTATGTGACTC+GGG 0.559082 4_3:+62471936 None:intergenic
TTGCGGGACGGAGGGAGTAG+TGG 0.560185 4_3:-62472484 Msa0654290:three_prime_UTR
GTAATAATTTAGTATGATTG+TGG 0.560611 4_3:+62472944 None:intergenic
CTGCAGGATGTTAATTGTGA+GGG 0.561151 4_3:-62472400 Msa0654290:three_prime_UTR
CTTCGGAGAACTGTGGACCT+GGG 0.567196 4_3:+62473762 None:intergenic
GAAGGGTATTACATTAGCCC+AGG 0.567931 4_3:-62475279 Msa0654290:intron
TGTATGTATTGCGGGACGGA+GGG 0.569402 4_3:+62472920 None:intergenic
TTGTCTCAATGCTGCACGGA+TGG 0.569745 4_3:-62472218 Msa0654290:three_prime_UTR
TCCTAAAAGTGGAAAATGCA+AGG 0.571235 4_3:-62475661 Msa0654290:intron
AATAGTTCATGTATGTATTG+CGG 0.573838 4_3:+62472911 None:intergenic
CTTAGGACATACATCAAACA+TGG 0.577045 4_3:-62473457 Msa0654290:CDS
CTGTTGTAGATATAATGGTT+GGG 0.577593 4_3:+62473118 None:intergenic
GAAGACTGTCTTGACAATGT+AGG 0.580932 4_3:-62474618 Msa0654290:CDS
GTCAAACAAATCATGTATTG+CGG 0.583095 4_3:-62472501 Msa0654290:three_prime_UTR
AAATCGTTGCTTTATGATGG+TGG 0.583566 4_3:-62472139 Msa0654290:three_prime_UTR
AACTTTGTCTCAATGCTGCA+CGG 0.584173 4_3:-62472222 Msa0654290:three_prime_UTR
CCACAAATTTGGCTTGAGGA+TGG 0.584912 4_3:+62474547 None:intergenic
ATCCTTTCTTCGGAGAACTG+TGG 0.586049 4_3:+62473755 None:intergenic
GGACATACATCAAACATGGA+TGG 0.590806 4_3:-62473453 Msa0654290:CDS
GCCATGAAATGTTGATGACC+AGG 0.591213 4_3:+62474569 None:intergenic
TGGAAATGTTCCAAATTCAG+AGG 0.592231 4_3:-62473253 Msa0654290:CDS
AGCTCCCAAACAACCAAACA+AGG 0.596345 4_3:+62471908 None:intergenic
AAATTGAAGCAACACTTGAA+AGG 0.596654 4_3:-62475392 Msa0654290:CDS
ACCAGGCATTAAGATATTGG+TGG 0.597715 4_3:+62474586 None:intergenic
AAAATTTGAGAAGGTCAAGT+GGG 0.600944 4_3:+62472872 None:intergenic
CAATACTTCTTCAAAGCTAG+AGG 0.600961 4_3:-62472170 Msa0654290:three_prime_UTR
CATCTCTCTTACTCAAACCA+AGG 0.603238 4_3:-62475856 Msa0654290:five_prime_UTR
ACTGCAGGATGTTAATTGTG+AGG 0.605083 4_3:-62472401 Msa0654290:three_prime_UTR
ATGTATGTATTGCGGGACGG+AGG 0.605733 4_3:+62472919 None:intergenic
GCATTTACTAAGATTAAACA+TGG 0.607561 4_3:+62472836 None:intergenic
AGTTTCTATTGGACAAACAA+AGG 0.608984 4_3:-62472332 Msa0654290:three_prime_UTR
GAAAGACTTAGGTTTGGCAA+AGG 0.612512 4_3:-62473843 Msa0654290:CDS
GAAACTCTTAAATCTAAGCA+TGG 0.613254 4_3:+62475362 None:intergenic
AATCATGTATTGCGGGACGG+AGG 0.617010 4_3:-62472493 Msa0654290:three_prime_UTR
TGATGACTTGGAAGGCGACA+AGG 0.619282 4_3:-62473076 Msa0654290:CDS
TCTTTACAGATGCTGTTGAG+AGG 0.626983 4_3:-62473652 Msa0654290:intron
ACAAACAAATAAAACTAACA+TGG 0.628841 4_3:-62472453 Msa0654290:three_prime_UTR
AGGCTATGTGATGACTTGGA+AGG 0.633610 4_3:-62473084 Msa0654290:CDS
AACCAATTAAATGGTACTCG+TGG 0.634167 4_3:-62473799 Msa0654290:CDS
TGTGCCACAAATTTGGCTTG+AGG 0.636253 4_3:+62474543 None:intergenic
AAAGTGTAGTTACACGTCTG+TGG 0.637874 4_3:-62472745 Msa0654290:three_prime_UTR
TTCGTATGTTGAGACAAGAA+GGG 0.638397 4_3:-62475296 Msa0654290:CDS
ATGATGTATATTAGGGACAG+AGG 0.640251 4_3:+62473719 None:intergenic
ACAAATCATGTATTGCGGGA+CGG 0.640721 4_3:-62472496 Msa0654290:three_prime_UTR
TTCATGTATGTATTGCGGGA+CGG 0.643051 4_3:+62472916 None:intergenic
GAAGTCATCCATTAGAGACA+CGG 0.643126 4_3:+62473140 None:intergenic
AACCCGAGTCACATAAAGTG+TGG 0.643938 4_3:-62471938 Msa0654290:three_prime_UTR
CAATTGAGAAGCTTCAAACA+AGG 0.656364 4_3:+62474653 None:intergenic
ATAGTAAGAATTATTACTAG+GGG 0.658427 4_3:-62472588 Msa0654290:three_prime_UTR
TGCATGGAAACAACTCAACA+AGG 0.663635 4_3:-62472284 Msa0654290:three_prime_UTR
TTAATTGTGAGGGTAATGAT+GGG 0.700457 4_3:-62472390 Msa0654290:three_prime_UTR
GACTTGGAAGGCGACAAGGT+AGG 0.702523 4_3:-62473072 Msa0654290:CDS
TGTTACATGAAAGATAACCC+AGG 0.716647 4_3:-62472355 Msa0654290:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAATTGTTTGTTT+AGG - chr4_3:62472475-62472494 Msa0654290:three_prime_UTR 10.0%
!!! TTAATTCTACTTTAAAATAT+TGG - chr4_3:62474621-62474640 Msa0654290:CDS 10.0%
!! TTTATTATAGAAACTAGAAT+AGG + chr4_3:62471927-62471946 None:intergenic 15.0%
!! TCAAACTTCAAATAAATTAT+AGG - chr4_3:62472550-62472569 Msa0654290:three_prime_UTR 15.0%
!! CAAACTTCAAATAAATTATA+GGG - chr4_3:62472551-62472570 Msa0654290:three_prime_UTR 15.0%
!!! AATTTTTATGAAAGTTAAGT+AGG + chr4_3:62473293-62473312 None:intergenic 15.0%
!!! TTTTTTCAAGAAAAAACAAA+TGG - chr4_3:62473413-62473432 Msa0654290:intron 15.0%
!!! AAAAAGTAGAAACTTTTTTT+GGG + chr4_3:62473491-62473510 None:intergenic 15.0%
!!! AAAAAAGTAGAAACTTTTTT+TGG + chr4_3:62473492-62473511 None:intergenic 15.0%
!!! TTTTTTTTTTAATGATTTGG+AGG + chr4_3:62473613-62473632 None:intergenic 15.0%
!!! GTTTAATTTTTATACATTGT+CGG + chr4_3:62473708-62473727 None:intergenic 15.0%
!!! GATGATATATTTGATTTTTA+TGG - chr4_3:62473948-62473967 Msa0654290:intron 15.0%
!!! TTTAATTTTTTATTTGAGTG+AGG + chr4_3:62474030-62474049 None:intergenic 15.0%
!!! GATTTTTTTTTTTACTAGAA+TGG + chr4_3:62474053-62474072 None:intergenic 15.0%
!! TTTAACCTCATTAATAAAAT+CGG - chr4_3:62474218-62474237 Msa0654290:intron 15.0%
!! TACTTAATTGTGATTAATTT+GGG - chr4_3:62474301-62474320 Msa0654290:intron 15.0%
!!! TTATTTTAAATAATTACTGC+AGG - chr4_3:62475227-62475246 Msa0654290:intron 15.0%
!!! GTTGATATTTTAATAAAATG+AGG - chr4_3:62475835-62475854 Msa0654290:exon 15.0%
!!! AAACAAAAGCACAAATATTT+TGG - chr4_3:62471880-62471899 Msa0654290:three_prime_UTR 20.0%
!!! TCTTTTTCTTTAGTTTCATA+TGG - chr4_3:62472026-62472045 Msa0654290:three_prime_UTR 20.0%
!! TCCTAAATGATTAATTGAAA+GGG + chr4_3:62472091-62472110 None:intergenic 20.0%
!! ATCCTAAATGATTAATTGAA+AGG + chr4_3:62472092-62472111 None:intergenic 20.0%
!!! ATTAGATTGGTTTTTAACTT+TGG - chr4_3:62472433-62472452 Msa0654290:three_prime_UTR 20.0%
!! CTTAGATAAGTATATGTAAT+TGG + chr4_3:62472840-62472859 None:intergenic 20.0%
!!! TTTTTTATCAAAATGACACA+TGG - chr4_3:62473518-62473537 Msa0654290:CDS 20.0%
!!! ATATGTATGACTTTAAAAGT+AGG + chr4_3:62473651-62473670 None:intergenic 20.0%
!! TGATGTAATCATTAGTAATA+CGG + chr4_3:62473754-62473773 None:intergenic 20.0%
!! GATTACATCATTTATAATCA+TGG - chr4_3:62473764-62473783 Msa0654290:CDS 20.0%
!! TTATAATCATGGTATATAGA+TGG - chr4_3:62473775-62473794 Msa0654290:CDS 20.0%
!! ATCATCAATGATGTATATTA+GGG + chr4_3:62473934-62473953 None:intergenic 20.0%
!! TATCATCAATGATGTATATT+AGG + chr4_3:62473935-62473954 None:intergenic 20.0%
!!! GATTTTATTAATGAGGTTAA+AGG + chr4_3:62474219-62474238 None:intergenic 20.0%
!!! CTATAAAGATTTAGAATGAA+GGG + chr4_3:62474248-62474267 None:intergenic 20.0%
!!! ACTATAAAGATTTAGAATGA+AGG + chr4_3:62474249-62474268 None:intergenic 20.0%
!! CTACTTAATTGTGATTAATT+TGG - chr4_3:62474300-62474319 Msa0654290:intron 20.0%
!! ATTAATGAAGTTAGAAACAT+AGG + chr4_3:62474606-62474625 None:intergenic 20.0%
!!! ATTTTTCTAGGGAATTTTTT+TGG + chr4_3:62474657-62474676 None:intergenic 20.0%
!! GTAATAATTTAGTATGATTG+TGG + chr4_3:62474702-62474721 None:intergenic 20.0%
!! CATAGTAAGAATTATTACTA+GGG - chr4_3:62475054-62475073 Msa0654290:intron 20.0%
!! ATAGTAAGAATTATTACTAG+GGG - chr4_3:62475055-62475074 Msa0654290:intron 20.0%
!!! TTTTCTTAATCTGTATGAAA+CGG - chr4_3:62475120-62475139 Msa0654290:intron 20.0%
!! ACAAACAAATAAAACTAACA+TGG - chr4_3:62475190-62475209 Msa0654290:intron 20.0%
!!! TTTTTGATACATTTTGAGTT+AGG - chr4_3:62475899-62475918 Msa0654290:five_prime_UTR 20.0%
! AAATCCAAAGCTTATTGTAT+TGG - chr4_3:62471754-62471773 Msa0654290:three_prime_UTR 25.0%
! TGAAGAAAATGTTGAATGAT+TGG + chr4_3:62471861-62471880 None:intergenic 25.0%
! ATTCAAGACAGTAGTTAAAT+TGG + chr4_3:62471951-62471970 None:intergenic 25.0%
! CTTAATTAGCTACAAGTTAA+TGG + chr4_3:62472064-62472083 None:intergenic 25.0%
! ACCCTTTCAATTAATCATTT+AGG - chr4_3:62472087-62472106 Msa0654290:three_prime_UTR 25.0%
! TTGAGTATAGAAAATTTGTG+AGG + chr4_3:62472648-62472667 None:intergenic 25.0%
!! TTTAGGATATCAACAATGTT+AGG + chr4_3:62472732-62472751 None:intergenic 25.0%
!!! ACATTTTCTGAGGATATAAA+TGG - chr4_3:62472959-62472978 Msa0654290:three_prime_UTR 25.0%
!!! TGAGGATATAAATGGATTAA+TGG - chr4_3:62472967-62472986 Msa0654290:three_prime_UTR 25.0%
! TTTATGAATTGGATACATCA+AGG + chr4_3:62473128-62473147 None:intergenic 25.0%
!!! CTTGACAAAGTTTTATGAAT+TGG + chr4_3:62473139-62473158 None:intergenic 25.0%
! TTCAAAAATAGACACTCAAA+TGG - chr4_3:62473219-62473238 Msa0654290:CDS 25.0%
!!! GTACGTTTTTAAAAGATTCA+AGG - chr4_3:62473314-62473333 Msa0654290:intron 25.0%
! GTATTTGATTCATTAACATG+TGG + chr4_3:62473340-62473359 None:intergenic 25.0%
! TTTCAAGAAAAAACAAATGG+CGG - chr4_3:62473416-62473435 Msa0654290:intron 25.0%
!! CTTAATAGCATTCTTTTGTT+TGG - chr4_3:62474072-62474091 Msa0654290:intron 25.0%
!!! GAATACCGATTTTATTAATG+AGG + chr4_3:62474226-62474245 None:intergenic 25.0%
! ATGTGCCAAAGAATAAATAT+TGG - chr4_3:62474675-62474694 Msa0654290:CDS 25.0%
! AATAGTTCATGTATGTATTG+CGG + chr4_3:62474735-62474754 None:intergenic 25.0%
! ATCTGCTTAAAAATTTGAGA+AGG + chr4_3:62474783-62474802 None:intergenic 25.0%
! GCATTTACTAAGATTAAACA+TGG + chr4_3:62474810-62474829 None:intergenic 25.0%
!! ATGCTTTTGTAAAACTTTTG+TGG - chr4_3:62474826-62474845 Msa0654290:intron 25.0%
!!! CTTACATACACTTTTTACAT+GGG + chr4_3:62474853-62474872 None:intergenic 25.0%
!!! TCTTACATACACTTTTTACA+TGG + chr4_3:62474854-62474873 None:intergenic 25.0%
! GCATAGTAAGAATTATTACT+AGG - chr4_3:62475053-62475072 Msa0654290:intron 25.0%
!! TTAGTAAAAGATGATGGTAA+TGG + chr4_3:62475402-62475421 None:intergenic 25.0%
! CAAAGTTTAGTAAAAGATGA+TGG + chr4_3:62475408-62475427 None:intergenic 25.0%
! CCATCATAATTGTACATTAT+AGG + chr4_3:62475453-62475472 None:intergenic 25.0%
! CCTATAATGTACAATTATGA+TGG - chr4_3:62475450-62475469 Msa0654290:CDS 25.0%
GAAACCAATACAATAAGCTT+TGG + chr4_3:62471761-62471780 None:intergenic 30.0%
AAACTAGAATAGGCATGTTT+GGG + chr4_3:62471917-62471936 None:intergenic 30.0%
CAAATTCATACTCCTAAAAG+TGG - chr4_3:62471971-62471990 Msa0654290:three_prime_UTR 30.0%
! TATAAGACATGCAAAAGCAT+TGG - chr4_3:62472120-62472139 Msa0654290:three_prime_UTR 30.0%
TACAGACGAATGTTTATCTA+TGG - chr4_3:62472183-62472202 Msa0654290:three_prime_UTR 30.0%
AAATTGAAGCAACACTTGAA+AGG - chr4_3:62472251-62472270 Msa0654290:three_prime_UTR 30.0%
GAAACTCTTAAATCTAAGCA+TGG + chr4_3:62472284-62472303 None:intergenic 30.0%
TTCCAGAGAAATGAATATCA+AGG - chr4_3:62472301-62472320 Msa0654290:three_prime_UTR 30.0%
CCAATCTAATCAATGTAGAT+TGG + chr4_3:62472423-62472442 None:intergenic 30.0%
! CCAATCTACATTGATTAGAT+TGG - chr4_3:62472420-62472439 Msa0654290:three_prime_UTR 30.0%
!! GATAAGTTTTGTGACAACAA+AGG - chr4_3:62472923-62472942 Msa0654290:three_prime_UTR 30.0%
! TTTTGTGATTGCATTGTTGT+TGG + chr4_3:62473169-62473188 None:intergenic 30.0%
TAATCATGGTATATAGATGG+TGG - chr4_3:62473778-62473797 Msa0654290:CDS 30.0%
CTAGAGATGAACCAATTAAA+TGG - chr4_3:62473835-62473854 Msa0654290:CDS 30.0%
! TAACCAATGAATTTGCTCTT+AGG - chr4_3:62474169-62474188 Msa0654290:intron 30.0%
GAAGATAGTATGTTACACAT+AGG + chr4_3:62474281-62474300 None:intergenic 30.0%
TGGGTGTTAATTAATTACAG+TGG - chr4_3:62474320-62474339 Msa0654290:intron 30.0%
GGGTGTTAATTAATTACAGT+GGG - chr4_3:62474321-62474340 Msa0654290:intron 30.0%
TTCAAATACTCCTCTGAATT+TGG + chr4_3:62474403-62474422 None:intergenic 30.0%
CTGTTGTAGATATAATGGTT+GGG + chr4_3:62474528-62474547 None:intergenic 30.0%
TTTGGCTGTTGTAGATATAA+TGG + chr4_3:62474533-62474552 None:intergenic 30.0%
!! ATCACATAGCCTTAGAATTT+TGG + chr4_3:62474551-62474570 None:intergenic 30.0%
! TTCTTTGGCACATTTTTCTA+GGG + chr4_3:62474668-62474687 None:intergenic 30.0%
! ATTCTTTGGCACATTTTTCT+AGG + chr4_3:62474669-62474688 None:intergenic 30.0%
GTGGTCCAATATTTATTCTT+TGG + chr4_3:62474683-62474702 None:intergenic 30.0%
ATAGTTCATGTATGTATTGC+GGG + chr4_3:62474734-62474753 None:intergenic 30.0%
AAAATTTGAGAAGGTCAAGT+GGG + chr4_3:62474774-62474793 None:intergenic 30.0%
AAAAATTTGAGAAGGTCAAG+TGG + chr4_3:62474775-62474794 None:intergenic 30.0%
!! CATTCACGTTTTTCAATTCA+TGG + chr4_3:62475007-62475026 None:intergenic 30.0%
GTCAAACAAATCATGTATTG+CGG - chr4_3:62475142-62475161 Msa0654290:intron 30.0%
TCAAACAAATCATGTATTGC+GGG - chr4_3:62475143-62475162 Msa0654290:intron 30.0%
TTAATTGTGAGGGTAATGAT+GGG - chr4_3:62475253-62475272 Msa0654290:intron 30.0%
AGTTTCTATTGGACAAACAA+AGG - chr4_3:62475311-62475330 Msa0654290:CDS 30.0%
AGACTATTAGTAATGTGCCT+TGG + chr4_3:62471807-62471826 None:intergenic 35.0%
GGCACATTACTAATAGTCTA+TGG - chr4_3:62471808-62471827 Msa0654290:three_prime_UTR 35.0%
GAAACTAGAATAGGCATGTT+TGG + chr4_3:62471918-62471937 None:intergenic 35.0%
TCCTAAAAGTGGAAAATGCA+AGG - chr4_3:62471982-62472001 Msa0654290:three_prime_UTR 35.0%
!! ACCTTGCATTTTCCACTTTT+AGG + chr4_3:62471986-62472005 None:intergenic 35.0%
AGACGAATGTTTATCTATGG+TGG - chr4_3:62472186-62472205 Msa0654290:three_prime_UTR 35.0%
GTGGATTCCATCAAAAGATT+AGG - chr4_3:62472205-62472224 Msa0654290:three_prime_UTR 35.0%
TTCCATCAAAAGATTAGGCA+TGG - chr4_3:62472210-62472229 Msa0654290:three_prime_UTR 35.0%
!! CATGGAGTACAATTTTGAAG+AGG - chr4_3:62472228-62472247 Msa0654290:three_prime_UTR 35.0%
GTCCTTGATATTCATTTCTC+TGG + chr4_3:62472306-62472325 None:intergenic 35.0%
TTTCGTATGTTGAGACAAGA+AGG - chr4_3:62472346-62472365 Msa0654290:three_prime_UTR 35.0%
TTCGTATGTTGAGACAAGAA+GGG - chr4_3:62472347-62472366 Msa0654290:three_prime_UTR 35.0%
ACTTGAAAGCAAACTAACCT+GGG + chr4_3:62472384-62472403 None:intergenic 35.0%
TACTTGAAAGCAAACTAACC+TGG + chr4_3:62472385-62472404 None:intergenic 35.0%
!! AGGTTAGTTTGCTTTCAAGT+AGG - chr4_3:62472384-62472403 Msa0654290:three_prime_UTR 35.0%
TTGTCTAATAGAAACCCATG+TGG - chr4_3:62472676-62472695 Msa0654290:three_prime_UTR 35.0%
!!! AGTGCTGTGTGACTTTTTTT+AGG - chr4_3:62472701-62472720 Msa0654290:three_prime_UTR 35.0%
AAAAAACAAGGTCTGGTCAT+GGG + chr4_3:62472880-62472899 None:intergenic 35.0%
TAAGATGCAGCGAAAAAACA+AGG + chr4_3:62472892-62472911 None:intergenic 35.0%
! ACTCAAGCATACATTTTCTG+AGG - chr4_3:62472949-62472968 Msa0654290:three_prime_UTR 35.0%
CAATTGAGAAGCTTCAAACA+AGG + chr4_3:62472993-62473012 None:intergenic 35.0%
ATGACCAGGCATTAAGATAT+TGG + chr4_3:62473063-62473082 None:intergenic 35.0%
AGTTAAGTAGGACTCTTACT+TGG + chr4_3:62473281-62473300 None:intergenic 35.0%
!! TATAGATGGTGGAAAGACTT+AGG - chr4_3:62473789-62473808 Msa0654290:CDS 35.0%
AACCAATTAAATGGTACTCG+TGG - chr4_3:62473844-62473863 Msa0654290:CDS 35.0%
ATGATGTATATTAGGGACAG+AGG + chr4_3:62473927-62473946 None:intergenic 35.0%
TGTCCTAAGAGCAAATTCAT+TGG + chr4_3:62474175-62474194 None:intergenic 35.0%
CTTAGGACATACATCAAACA+TGG - chr4_3:62474186-62474205 Msa0654290:intron 35.0%
CTTTCTTGCAAGAAGCAAAA+TGG - chr4_3:62474359-62474378 Msa0654290:intron 35.0%
TTGCAAGAAGCAAAATGGTT+TGG - chr4_3:62474364-62474383 Msa0654290:intron 35.0%
TGGAAATGTTCCAAATTCAG+AGG - chr4_3:62474390-62474409 Msa0654290:intron 35.0%
GCTGTTGTAGATATAATGGT+TGG + chr4_3:62474529-62474548 None:intergenic 35.0%
CTACAACAGCCAAAATTCTA+AGG - chr4_3:62474539-62474558 Msa0654290:CDS 35.0%
!!! GGGAATTTTTTTGGTACATG+TGG + chr4_3:62474648-62474667 None:intergenic 35.0%
! AGACAACACTATTGGTCTAT+TGG + chr4_3:62474942-62474961 None:intergenic 35.0%
! ATCATCTGAGACAACACTAT+TGG + chr4_3:62474950-62474969 None:intergenic 35.0%
TAGTGTTGTCTCAGATGATT+AGG - chr4_3:62474951-62474970 Msa0654290:intron 35.0%
GTTAATTGTGAGGGTAATGA+TGG - chr4_3:62475252-62475271 Msa0654290:intron 35.0%
TGTTACATGAAAGATAACCC+AGG - chr4_3:62475288-62475307 Msa0654290:CDS 35.0%
TTTGTCCAATAGAAACTCCT+GGG + chr4_3:62475308-62475327 None:intergenic 35.0%
GTGAACAAATTTCAGATGCA+TGG - chr4_3:62475343-62475362 Msa0654290:CDS 35.0%
CAATACTTCTTCAAAGCTAG+AGG - chr4_3:62475473-62475492 Msa0654290:CDS 35.0%
! GTGAAATCGTTGCTTTATGA+TGG - chr4_3:62475501-62475520 Msa0654290:CDS 35.0%
! AAATCGTTGCTTTATGATGG+TGG - chr4_3:62475504-62475523 Msa0654290:CDS 35.0%
TATCCACACTTTATGTGACT+CGG + chr4_3:62475711-62475730 None:intergenic 35.0%
! ATACTCCTTGTTTGGTTGTT+TGG - chr4_3:62475730-62475749 Msa0654290:CDS 35.0%
! TACTCCTTGTTTGGTTGTTT+GGG - chr4_3:62475731-62475750 Msa0654290:CDS 35.0%
AGAATAGCATAGACACACTT+TGG - chr4_3:62475769-62475788 Msa0654290:CDS 35.0%
CATCTCTCTTACTCAAACCA+AGG - chr4_3:62471787-62471806 Msa0654290:three_prime_UTR 40.0%
! AGCATTGGATGAACTTAAGC+AGG - chr4_3:62472135-62472154 Msa0654290:three_prime_UTR 40.0%
! CTCCATGCCTAATCTTTTGA+TGG + chr4_3:62472215-62472234 None:intergenic 40.0%
AAAAACAAGGTCTGGTCATG+GGG + chr4_3:62472879-62472898 None:intergenic 40.0%
GAAAAAACAAGGTCTGGTCA+TGG + chr4_3:62472881-62472900 None:intergenic 40.0%
GCTTCTCAATTGAGCATAGA+AGG - chr4_3:62473001-62473020 Msa0654290:three_prime_UTR 40.0%
GAAGACTGTCTTGACAATGT+AGG - chr4_3:62473025-62473044 Msa0654290:three_prime_UTR 40.0%
ACCAGGCATTAAGATATTGG+TGG + chr4_3:62473060-62473079 None:intergenic 40.0%
TGCAATCACAAAATGCAGCA+CGG - chr4_3:62473176-62473195 Msa0654290:CDS 40.0%
GGTTTCATCATCACATCTCA+AGG - chr4_3:62473240-62473259 Msa0654290:CDS 40.0%
! ATGGTGGAAAGACTTAGGTT+TGG - chr4_3:62473794-62473813 Msa0654290:CDS 40.0%
GAAAGACTTAGGTTTGGCAA+AGG - chr4_3:62473800-62473819 Msa0654290:CDS 40.0%
GACCACGAGTACCATTTAAT+TGG + chr4_3:62473849-62473868 None:intergenic 40.0%
TAAATGGTACTCGTGGTCAA+TGG - chr4_3:62473851-62473870 Msa0654290:CDS 40.0%
TGTGAGCTCAATCCTTTCTT+CGG + chr4_3:62473901-62473920 None:intergenic 40.0%
TCTTTACAGATGCTGTTGAG+AGG - chr4_3:62473991-62474010 Msa0654290:intron 40.0%
!! TTCTTTTGTTTGGACGCAGA+TGG - chr4_3:62474082-62474101 Msa0654290:intron 40.0%
!! TCTTTTGTTTGGACGCAGAT+GGG - chr4_3:62474083-62474102 Msa0654290:intron 40.0%
AAACACACTTTCATGCAGTC+TGG + chr4_3:62474132-62474151 None:intergenic 40.0%
! CTGCATGAAAGTGTGTTTCA+AGG - chr4_3:62474134-62474153 Msa0654290:intron 40.0%
GGACATACATCAAACATGGA+TGG - chr4_3:62474190-62474209 Msa0654290:intron 40.0%
!! GAAGCAAAATGGTTTGGTTC+TGG - chr4_3:62474370-62474389 Msa0654290:intron 40.0%
AAGAATGCCATAGTAAGCAC+TGG - chr4_3:62474421-62474440 Msa0654290:intron 40.0%
! TGTGCATGCTTTCTTTTGCA+TGG - chr4_3:62474459-62474478 Msa0654290:intron 40.0%
! GTGCATGCTTTCTTTTGCAT+GGG - chr4_3:62474460-62474479 Msa0654290:intron 40.0%
TGAGAAAACCGTGTCTCTAA+TGG - chr4_3:62474495-62474514 Msa0654290:intron 40.0%
GAAGTCATCCATTAGAGACA+CGG + chr4_3:62474506-62474525 None:intergenic 40.0%
! TCTAAGGCTATGTGATGACT+TGG - chr4_3:62474555-62474574 Msa0654290:CDS 40.0%
TTCATGTATGTATTGCGGGA+CGG + chr4_3:62474730-62474749 None:intergenic 40.0%
AAAGTGTAGTTACACGTCTG+TGG - chr4_3:62474898-62474917 Msa0654290:intron 40.0%
ACAAATCATGTATTGCGGGA+CGG - chr4_3:62475147-62475166 Msa0654290:intron 40.0%
ACTGCAGGATGTTAATTGTG+AGG - chr4_3:62475242-62475261 Msa0654290:intron 40.0%
CTGCAGGATGTTAATTGTGA+GGG - chr4_3:62475243-62475262 Msa0654290:intron 40.0%
GATAACCCAGGAGTTTCTAT+TGG - chr4_3:62475300-62475319 Msa0654290:CDS 40.0%
GTTTGTCCAATAGAAACTCC+TGG + chr4_3:62475309-62475328 None:intergenic 40.0%
TGCATGGAAACAACTCAACA+AGG - chr4_3:62475359-62475378 Msa0654290:CDS 40.0%
AACTTTGTCTCAATGCTGCA+CGG - chr4_3:62475421-62475440 Msa0654290:CDS 40.0%
TGCAGAATGCTTTGATGTTG+GGG + chr4_3:62475550-62475569 None:intergenic 40.0%
CTGCAGAATGCTTTGATGTT+GGG + chr4_3:62475551-62475570 None:intergenic 40.0%
ACTGCAGAATGCTTTGATGT+TGG + chr4_3:62475552-62475571 None:intergenic 40.0%
ATCCACACTTTATGTGACTC+GGG + chr4_3:62475710-62475729 None:intergenic 40.0%
GTGTGGATATACTCCTTGTT+TGG - chr4_3:62475722-62475741 Msa0654290:CDS 40.0%
!! GAAGGGTATTACATTAGCCC+AGG - chr4_3:62472364-62472383 Msa0654290:three_prime_UTR 45.0%
TGCAGCGAAAAAACAAGGTC+TGG + chr4_3:62472887-62472906 None:intergenic 45.0%
GCCACCAATATCTTAATGCC+TGG - chr4_3:62473056-62473075 Msa0654290:CDS 45.0%
GCCTGGTCATCAACATTTCA+TGG - chr4_3:62473073-62473092 Msa0654290:CDS 45.0%
!! GCCATGAAATGTTGATGACC+AGG + chr4_3:62473077-62473096 None:intergenic 45.0%
!! CCACAAATTTGGCTTGAGGA+TGG + chr4_3:62473099-62473118 None:intergenic 45.0%
CCATCCTCAAGCCAAATTTG+TGG - chr4_3:62473096-62473115 Msa0654290:CDS 45.0%
!! TGTGCCACAAATTTGGCTTG+AGG + chr4_3:62473103-62473122 None:intergenic 45.0%
CAAGGTGTGTGCCACAAATT+TGG + chr4_3:62473110-62473129 None:intergenic 45.0%
CTTAGGTTTGGCAAAGGACT+TGG - chr4_3:62473806-62473825 Msa0654290:CDS 45.0%
ATCCTTTCTTCGGAGAACTG+TGG + chr4_3:62473891-62473910 None:intergenic 45.0%
! GCTTTCTTTTGCATGGGTCA+AGG - chr4_3:62474466-62474485 Msa0654290:intron 45.0%
! AGGCTATGTGATGACTTGGA+AGG - chr4_3:62474559-62474578 Msa0654290:CDS 45.0%
GACTCGGGTTCAGTAAAATC+AGG + chr4_3:62475695-62475714 None:intergenic 45.0%
AACCCGAGTCACATAAAGTG+TGG - chr4_3:62475705-62475724 Msa0654290:CDS 45.0%
AGCTCCCAAACAACCAAACA+AGG + chr4_3:62475738-62475757 None:intergenic 45.0%
TTGTTTGGTTGTTTGGGAGC+TGG - chr4_3:62475737-62475756 Msa0654290:CDS 45.0%
TGTTTGGTTGTTTGGGAGCT+GGG - chr4_3:62475738-62475757 Msa0654290:CDS 45.0%
TTGTTTGGGAGCTGGGAATT+AGG - chr4_3:62475745-62475764 Msa0654290:CDS 45.0%
! GCATAGACACACTTTGGAAG+AGG - chr4_3:62475775-62475794 Msa0654290:CDS 45.0%
!!! TTTTAAGTTATTAATATTTT+AGG + chr4_3:62472749-62472768 None:intergenic 5.0%
!!! TAATTTTTTTTTTAATGATT+TGG + chr4_3:62473616-62473635 None:intergenic 5.0%
TCACACAGCACTTGCCACAT+GGG + chr4_3:62472693-62472712 None:intergenic 50.0%
GTCCACAGTTCTCCGAAGAA+AGG - chr4_3:62473886-62473905 Msa0654290:intron 50.0%
GTGCACTCCAGTGCTTACTA+TGG + chr4_3:62474431-62474450 None:intergenic 50.0%
! TGATGACTTGGAAGGCGACA+AGG - chr4_3:62474567-62474586 Msa0654290:CDS 50.0%
TGTATGTATTGCGGGACGGA+GGG + chr4_3:62474726-62474745 None:intergenic 50.0%
ATGTATGTATTGCGGGACGG+AGG + chr4_3:62474727-62474746 None:intergenic 50.0%
AATCATGTATTGCGGGACGG+AGG - chr4_3:62475150-62475169 Msa0654290:intron 50.0%
ATCATGTATTGCGGGACGGA+GGG - chr4_3:62475151-62475170 Msa0654290:intron 50.0%
TTGTCTCAATGCTGCACGGA+TGG - chr4_3:62475425-62475444 Msa0654290:CDS 50.0%
GTCACACAGCACTTGCCACA+TGG + chr4_3:62472694-62472713 None:intergenic 55.0%
! CAAATGGCGGAACCCCTTCT+CGG - chr4_3:62473429-62473448 Msa0654290:CDS 55.0%
TGGTCAATGGCATGTCTCCC+AGG - chr4_3:62473864-62473883 Msa0654290:intron 55.0%
CTTCGGAGAACTGTGGACCT+GGG + chr4_3:62473884-62473903 None:intergenic 55.0%
TCTTCGGAGAACTGTGGACC+TGG + chr4_3:62473885-62473904 None:intergenic 55.0%
GTTTGGACGCAGATGGGACT+TGG - chr4_3:62474089-62474108 Msa0654290:intron 55.0%
GACTTGGAAGGCGACAAGGT+AGG - chr4_3:62474571-62474590 Msa0654290:CDS 55.0%
CATACGCAGGGTCCGAGAAG+GGG + chr4_3:62473444-62473463 None:intergenic 60.0%
GCATACGCAGGGTCCGAGAA+GGG + chr4_3:62473445-62473464 None:intergenic 60.0%
CTTCTCGGACCCTGCGTATG+CGG - chr4_3:62473444-62473463 Msa0654290:CDS 60.0%
TTCTCGGACCCTGCGTATGC+GGG - chr4_3:62473445-62473464 Msa0654290:CDS 60.0%
CTAGAGCTCCCGCATACGCA+GGG + chr4_3:62473456-62473475 None:intergenic 60.0%
ACTAGAGCTCCCGCATACGC+AGG + chr4_3:62473457-62473476 None:intergenic 60.0%
CGCATACGCAGGGTCCGAGA+AGG + chr4_3:62473446-62473465 None:intergenic 65.0%
TTGCGGGACGGAGGGAGTAG+TGG - chr4_3:62475159-62475178 Msa0654290:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 62471727 62475938 62471727 ID=Msa0654290;Name=Msa0654290
chr4_3 mRNA 62471727 62475938 62471727 ID=Msa0654290-mRNA-1;Parent=Msa0654290;Name=Msa0654290-mRNA-1;_AED=0.06;_eAED=0.06;_QI=100|0.8|0.83|1|1|1|6|1323|434
chr4_3 exon 62471727 62473325 62471727 ID=Msa0654290-mRNA-1:exon:17329;Parent=Msa0654290-mRNA-1
chr4_3 exon 62473426 62473564 62473426 ID=Msa0654290-mRNA-1:exon:17328;Parent=Msa0654290-mRNA-1
chr4_3 exon 62473653 62473871 62473653 ID=Msa0654290-mRNA-1:exon:17327;Parent=Msa0654290-mRNA-1
chr4_3 exon 62474534 62474750 62474534 ID=Msa0654290-mRNA-1:exon:17326;Parent=Msa0654290-mRNA-1
chr4_3 exon 62475280 62475556 62475280 ID=Msa0654290-mRNA-1:exon:17325;Parent=Msa0654290-mRNA-1
chr4_3 exon 62475662 62475938 62475662 ID=Msa0654290-mRNA-1:exon:17324;Parent=Msa0654290-mRNA-1
chr4_3 five_prime_UTR 62475839 62475938 62475839 ID=Msa0654290-mRNA-1:five_prime_utr;Parent=Msa0654290-mRNA-1
chr4_3 CDS 62475662 62475838 62475662 ID=Msa0654290-mRNA-1:cds;Parent=Msa0654290-mRNA-1
chr4_3 CDS 62475280 62475556 62475280 ID=Msa0654290-mRNA-1:cds;Parent=Msa0654290-mRNA-1
chr4_3 CDS 62474534 62474750 62474534 ID=Msa0654290-mRNA-1:cds;Parent=Msa0654290-mRNA-1
chr4_3 CDS 62473653 62473871 62473653 ID=Msa0654290-mRNA-1:cds;Parent=Msa0654290-mRNA-1
chr4_3 CDS 62473426 62473564 62473426 ID=Msa0654290-mRNA-1:cds;Parent=Msa0654290-mRNA-1
chr4_3 CDS 62473050 62473325 62473050 ID=Msa0654290-mRNA-1:cds;Parent=Msa0654290-mRNA-1
chr4_3 three_prime_UTR 62471727 62473049 62471727 ID=Msa0654290-mRNA-1:three_prime_utr;Parent=Msa0654290-mRNA-1
Gene Sequence

>Msa0654290

ATGGCTTTTTCTCTTTCTTTCGCTGCTTCAACCAATCATTCAACATTTTCTTCAAACAAAAGCACAAATATTTTGGATATATCTATACCCAAACATGCCTATTCTAGTTTCTATAATAAAACCAATTTAACTACTGTCTTGAATCAAATTCATACTCCTAAAAGTGGAAAATGCAAGGATGATCTTCATATAAGACATGCAAAAGCATTGGATGAACTTAAGCAGGTATTTGTTAGAAATATAAGAAAAAATACAGACGAATGTTTATCTATGGTGGATTCCATCAAAAGATTAGGCATGGAGTACAATTTTGAAGAGGAAATTGAAGCAACACTTGAAAGGAAACACACCATGCTTAGATTTAAGAGTTTCCAGAGAAATGAATATCAAGGACTGTCACAAGCTGCATTTCAATTTCGTATGTTGAGACAAGAAGGGTATTACATTAGCCCAGACATATTTGATAAGTTTTGTGACAACAAAGGAAAACTCAAGCATACATTTTCTGAGGATATAAATGGATTAATGGCCTTGTTTGAAGCTTCTCAATTGAGCATAGAAGGAGAAGACTGTCTTGACAATGTAGGACAGTTCTGCCACCAATATCTTAATGCCTGGTCATCAACATTTCATGGCCATCCTCAAGCCAAATTTGTGGCACACACCTTGATATGGTGGAAAGACTTAGGTTTGGCAAAGGACTTGGAATTTGCTAGAGATGAACCAATTAAATGGTACTCGTGGTCAATGGCATGTCTCCCAGGTCCACAGTTCTCCGAAGAAAGGATTGAGCTCACAAAACCTCTGTCCCTAATATACATCATTGATGATATATTTGATTTTTATGGAAACATTGAAGAACTTACTCTCTTTACAGATGCTGTTGAGAGATGGGACTTGGCAGCTATTGAACAATTACCAGACTGCATGAAAGTGTGTTTCAAGGCTCTCTATGACATAACCAATGAATTTGCTCTTAGGACATACATCAAACATGGATGGAATCCTTTAACCTCATTAATAAAATCGTGGGTAAGACTCATGAATGCTTTCTTGCAAGAAGCAAAATGGTTTGGTTCTGGAAATGTTCCAAATTCAGAGGAGTATTTGAAGAATGCCATAGTAAGCACTGGAGTGCACGTGATACTTGTGCATGCTTTCTTTTGCATGGGTCAAGGTATAACTGAGAAAACCGTGTCTCTAATGGATGACTTCCCAACCATTATATCTACAACAGCCAAAATTCTAAGGCTATGTGATGACTTGGAAGGCGACAAGGTAGGAAAACATCACCTATGTTTCTAA
Protein sequence

>Msa0654290

MAFSLSFAASTNHSTFSSNKSTNILDISIPKHAYSSFYNKTNLTTVLNQIHTPKSGKCKDDLHIRHAKALDELKQVFVRNIRKNTDECLSMVDSIKRLGMEYNFEEEIEATLERKHTMLRFKSFQRNEYQGLSQAAFQFRMLRQEGYYISPDIFDKFCDNKGKLKHTFSEDINGLMALFEASQLSIEGEDCLDNVGQFCHQYLNAWSSTFHGHPQAKFVAHTLIWWKDLGLAKDLEFARDEPIKWYSWSMACLPGPQFSEERIELTKPLSLIYIIDDIFDFYGNIEELTLFTDAVERWDLAAIEQLPDCMKVCFKALYDITNEFALRTYIKHGWNPLTSLIKSWVRLMNAFLQEAKWFGSGNVPNSEEYLKNAIVSTGVHVILVHAFFCMGQGITEKTVSLMDDFPTIISTTAKILRLCDDLEGDKVGKHHLCF*