AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0696520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0696520 MtrunA17_Chr4g0048911 81.723 476 26 2 1 426 1 465 0.0 794
Msa0696520 MtrunA17_Chr6g0477111 45.226 398 162 3 87 428 2 399 3.36e-123 367
Msa0696520 MtrunA17_Chr3g0109031 43.932 412 174 4 74 428 13 424 1.77e-120 360
Msa0696520 MtrunA17_Chr3g0109021 43.932 412 174 4 74 428 13 424 5.52e-120 359
Msa0696520 MtrunA17_Chr3g0108981 44.862 399 163 4 87 428 2 400 7.58e-120 358
Msa0696520 MtrunA17_Chr3g0108961 44.862 399 163 4 87 428 2 400 7.58e-120 358
Msa0696520 MtrunA17_Chr3g0109001 42.958 426 184 5 60 428 2 425 1.57e-119 358
Msa0696520 MtrunA17_Chr3g0108941 44.068 413 173 5 74 428 13 425 2.76e-119 357
Msa0696520 MtrunA17_Chr3g0108991 42.892 415 180 4 74 431 13 427 1.03e-118 356
Msa0696520 MtrunA17_Chr3g0108951 42.892 415 180 4 74 431 13 427 1.03e-118 356
Msa0696520 MtrunA17_Chr3g0109011 44.221 398 166 3 87 428 2 399 1.26e-117 352
Msa0696520 MtrunA17_Chr3g0108971 43.860 399 168 3 87 429 2 400 1.27e-117 352
Msa0696520 MtrunA17_Chr3g0105691 43.204 412 177 4 74 428 46 457 3.08e-117 353
Msa0696520 MtrunA17_Chr3g0099031 43.059 425 184 4 60 428 2 424 5.97e-117 351
Msa0696520 MtrunA17_Chr2g0280581 41.943 422 187 4 63 426 5 426 3.40e-115 347
Msa0696520 MtrunA17_Chr4g0048921 42.336 411 177 5 66 422 65 469 1.08e-103 319
Msa0696520 MtrunA17_Chr6g0477121 46.547 333 122 3 152 428 10 342 4.09e-103 313
Msa0696520 MtrunA17_Chr2g0323151 34.367 387 192 4 89 422 4 381 6.36e-71 231
Msa0696520 MtrunA17_Chr4g0023911 35.194 412 211 10 65 422 71 480 1.33e-67 225
Msa0696520 MtrunA17_Chr4g0042111 31.863 408 218 7 71 422 58 461 2.48e-62 211
Msa0696520 MtrunA17_Chr2g0323131 30.717 446 250 9 27 422 30 466 2.88e-61 208
Msa0696520 MtrunA17_Chr2g0310331 33.333 387 200 5 89 422 105 486 8.56e-58 199
Msa0696520 MtrunA17_Chr2g0319051 30.414 411 228 6 71 426 10 417 2.32e-57 197
Msa0696520 MtrunA17_Chr8g0357411 29.216 421 238 5 55 421 74 488 2.06e-55 193
Msa0696520 MtrunA17_Chr6g0465851 28.501 407 224 7 87 433 31 430 1.01e-53 187
Msa0696520 MtrunA17_Chr6g0451711 26.667 420 245 6 72 433 55 469 1.09e-53 187
Msa0696520 MtrunA17_Chr6g0465911 28.501 407 224 7 87 433 66 465 2.41e-53 187
Msa0696520 MtrunA17_Chr2g0319041 28.235 425 243 10 60 426 40 460 5.02e-53 186
Msa0696520 MtrunA17_Chr6g0466051 28.256 407 225 7 87 433 66 465 9.46e-53 185
Msa0696520 MtrunA17_Chr6g0465441 28.440 436 242 10 61 433 37 465 1.10e-52 185
Msa0696520 MtrunA17_Chr5g0425371 27.543 403 229 5 89 433 68 465 2.81e-51 181
Msa0696520 MtrunA17_Chr3g0082691 27.569 399 232 3 84 426 59 456 2.63e-50 178
Msa0696520 MtrunA17_Chr5g0431651 27.529 425 236 6 64 422 33 451 2.64e-50 178
Msa0696520 MtrunA17_Chr7g0237011 26.980 404 230 5 89 433 13 410 4.07e-50 177
Msa0696520 MtrunA17_Chr7g0236961 26.980 404 230 5 89 433 13 410 4.80e-50 177
Msa0696520 MtrunA17_Chr3g0105071 27.692 390 218 4 91 422 73 456 5.18e-50 178
Msa0696520 MtrunA17_Chr4g0024691 27.970 404 235 4 72 422 60 460 5.58e-50 177
Msa0696520 MtrunA17_Chr5g0431571 28.710 411 225 5 78 426 48 452 2.49e-49 176
Msa0696520 MtrunA17_Chr4g0008621 28.101 395 219 9 49 384 37 425 6.34e-47 167
Msa0696520 MtrunA17_Chr2g0310401 43.434 198 112 0 225 422 135 332 1.23e-46 166
Msa0696520 MtrunA17_Chr7g0237001 27.475 404 228 8 89 433 68 465 5.99e-45 164
Msa0696520 MtrunA17_Chr7g0237041 27.475 404 228 8 89 433 68 465 5.99e-45 164
Msa0696520 MtrunA17_Chr7g0237081 27.475 404 228 8 89 433 68 465 5.99e-45 164
Msa0696520 MtrunA17_Chr7g0237051 27.475 404 228 8 89 433 68 465 5.99e-45 164
Msa0696520 MtrunA17_Chr7g0236991 27.475 404 228 8 89 433 68 465 6.76e-45 164
Msa0696520 MtrunA17_Chr6g0466111 30.952 294 140 4 87 323 31 318 5.72e-41 149
Msa0696520 MtrunA17_Chr6g0465931 30.952 294 140 4 87 323 31 318 6.02e-41 148
Msa0696520 MtrunA17_Chr6g0465981 30.952 294 140 4 87 323 66 353 1.34e-40 148
Msa0696520 MtrunA17_Chr6g0465871 30.952 294 140 4 87 323 66 353 1.34e-40 148
Msa0696520 MtrunA17_Chr6g0466081 30.952 294 140 4 87 323 66 353 1.34e-40 148
Msa0696520 MtrunA17_Chr7g0217361 37.681 207 112 5 225 422 11 209 1.68e-37 138
Msa0696520 MtrunA17_Chr8g0357231 40.881 159 91 2 265 421 1 158 3.27e-36 134
Msa0696520 MtrunA17_Chr7g0237031 30.479 292 140 7 89 323 13 298 2.06e-34 130
Msa0696520 MtrunA17_Chr7g0236951 30.479 292 140 7 89 323 13 298 2.94e-34 130
Msa0696520 MtrunA17_Chr7g0236971 29.858 211 146 1 225 433 103 313 1.25e-31 124
Msa0696520 MtrunA17_Chr2g0319081 29.665 209 145 2 225 432 140 347 7.16e-29 117
Msa0696520 MtrunA17_Chr8g0336181 31.604 212 142 2 217 426 249 459 1.90e-27 114
Msa0696520 MtrunA17_Chr8g0336181 36.232 138 78 3 71 204 51 182 1.02e-18 88.6
Msa0696520 MtrunA17_Chr7g0237061 29.412 170 116 1 265 434 2 167 7.00e-23 96.7
Msa0696520 MtrunA17_Chr5g0445511 26.383 235 118 3 171 352 2 234 1.49e-22 95.9
Msa0696520 MtrunA17_Chr6g0466031 29.070 172 118 3 265 433 2 172 1.89e-22 96.3
Msa0696520 MtrunA17_Chr6g0466021 36.879 141 79 3 87 223 66 200 3.32e-21 91.3
Msa0696520 MtrunA17_Chr4g0050281 61.194 67 24 1 7 71 37 103 1.35e-18 81.3
Msa0696520 MtrunA17_Chr7g0237071 33.871 124 73 2 89 208 13 131 1.56e-17 81.3
Msa0696520 MtrunA17_Chr2g0310121 27.225 191 137 2 235 424 492 681 6.64e-17 83.2
Msa0696520 MtrunA17_Chr3g0101681 26.316 190 138 2 235 423 493 681 2.51e-16 81.6
Msa0696520 MtrunA17_Chr2g0319071 38.333 120 68 1 89 208 46 159 9.65e-16 79.3
Msa0696520 MtrunA17_Chr2g0319071 28.244 131 90 3 298 426 264 392 2.57e-13 71.6
Msa0696520 MtrunA17_Chr2g0281651 26.733 202 141 5 225 424 68 264 5.26e-15 76.6
Msa0696520 MtrunA17_Chr8g0357221 28.402 169 115 3 55 223 74 236 1.31e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0696520 AT1G61680.1 36.929 482 224 8 8 426 17 481 2.12e-102 316
Msa0696520 AT1G61680.2 36.307 482 220 9 8 426 17 474 2.94e-98 305
Msa0696520 AT4G16740.1 30.653 398 204 11 87 422 76 463 2.28e-49 176
Msa0696520 AT2G24210.1 28.507 442 244 11 56 433 66 499 6.72e-47 170
Msa0696520 AT4G16730.2 38.191 199 123 0 224 422 191 389 4.25e-44 161
Msa0696520 AT4G16730.2 37.374 99 59 2 91 189 1 96 2.51e-13 72.0
Msa0696520 AT4G16730.1 38.191 199 123 0 224 422 237 435 7.58e-44 161
Msa0696520 AT4G16730.1 32.117 137 84 3 76 206 26 159 1.38e-14 76.3
Msa0696520 AT3G25820.1 29.032 403 222 11 80 422 91 489 2.14e-39 149
Msa0696520 AT3G25830.1 29.032 403 222 11 80 422 91 489 2.14e-39 149
Msa0696520 AT3G25810.1 29.177 401 219 11 83 422 95 491 2.33e-39 149
Msa0696520 AT4G16740.2 28.793 323 162 7 87 348 76 391 1.14e-32 127
Msa0696520 AT1G33750.1 24.121 398 240 5 83 424 113 504 6.59e-32 128
Msa0696520 AT3G14520.1 21.981 414 264 7 65 422 92 502 1.40e-31 127
Msa0696520 AT3G29410.2 24.619 394 232 6 87 422 11 397 3.06e-29 119
Msa0696520 AT3G29410.1 24.760 416 241 8 72 422 94 502 3.85e-29 120
Msa0696520 AT5G48110.1 35.323 201 127 3 223 422 42 240 5.28e-28 114
Msa0696520 AT5G23960.1 25.598 418 244 11 65 422 37 447 9.90e-28 115
Msa0696520 AT1G70080.1 32.673 202 131 3 224 422 310 509 2.37e-27 115
Msa0696520 AT3G25820.2 27.353 340 186 9 80 361 91 427 6.80e-27 112
Msa0696520 AT2G23230.2 24.146 410 249 8 83 433 105 511 4.48e-26 111
Msa0696520 AT4G13300.1 23.969 388 235 8 91 422 69 452 2.71e-25 108
Msa0696520 AT2G23230.1 24.402 418 246 10 76 433 99 506 8.29e-25 107
Msa0696520 AT5G44630.1 24.190 401 240 9 79 422 63 456 1.36e-24 106
Msa0696520 AT4G20200.1 23.059 425 249 10 68 424 91 505 3.46e-23 102
Msa0696520 AT3G14540.1 20.241 415 270 8 65 422 89 499 7.64e-23 101
Msa0696520 AT4G16740.4 27.941 272 129 6 87 297 76 341 1.17e-22 99.4
Msa0696520 AT4G16740.3 27.941 272 129 6 87 297 76 341 1.17e-22 99.4
Msa0696520 AT4G20210.1 24.311 399 241 7 83 424 107 501 1.66e-22 100
Msa0696520 AT3G32030.1 20.241 415 269 7 67 422 91 502 3.44e-22 99.8
Msa0696520 AT5G23960.2 29.703 202 134 3 225 422 248 445 3.18e-21 96.7
Msa0696520 AT5G23960.2 31.544 149 101 1 65 213 37 184 3.98e-17 84.0
Msa0696520 AT1G79460.1 29.534 193 134 2 233 424 488 679 6.04e-21 96.3
Msa0696520 AT3G14490.1 26.263 198 145 1 225 422 303 499 7.37e-21 95.5
Msa0696520 AT1G31950.1 25.962 208 141 4 222 422 303 504 6.76e-20 92.8
Msa0696520 AT4G20230.1 22.977 383 236 7 98 424 126 505 6.76e-20 92.8
Msa0696520 AT4G20230.2 22.977 383 236 7 98 424 138 517 6.77e-20 92.8
Msa0696520 AT1G31950.3 25.962 208 141 4 222 422 304 505 6.95e-20 92.8
Msa0696520 AT1G31950.2 25.962 208 141 4 222 422 304 505 6.99e-20 92.4
Msa0696520 AT3G14540.2 25.616 203 148 3 222 422 298 499 5.18e-19 89.7
Msa0696520 AT3G29110.1 24.872 390 234 9 91 424 112 498 6.23e-19 89.7
Msa0696520 AT4G15870.1 30.244 205 129 4 222 424 306 498 6.67e-19 89.7
Msa0696520 AT3G25820.4 27.174 276 140 9 80 297 91 363 1.49e-16 81.6
Msa0696520 AT3G25820.3 27.174 276 140 9 80 297 91 363 1.49e-16 81.6
Msa0696520 AT3G29190.1 26.961 204 147 2 225 426 301 504 1.63e-16 82.4
Msa0696520 AT4G13280.2 27.723 202 143 2 223 422 252 452 3.55e-15 78.2
Msa0696520 AT1G48800.1 23.786 412 232 14 83 426 112 509 7.62e-15 77.0
Msa0696520 AT1G66020.1 27.228 202 145 2 225 424 298 499 3.37e-13 72.0
Msa0696520 AT1G61120.1 27.230 213 147 4 215 424 477 684 4.44e-13 72.0
Msa0696520 AT1G48820.1 24.082 245 161 6 183 424 242 464 2.00e-11 66.2

Find 142 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATACTCCTCTGAATT+TGG 0.087434 4_4:+66476997 None:intergenic
AAACAAAAGCACAAATATTT+TGG 0.114515 4_4:-66479461 Msa0696520:CDS
ATCACATAGCCTTAGAATTT+TGG 0.127459 4_4:+66476849 None:intergenic
TATCATCAATGATGTATATT+AGG 0.172952 4_4:+66477465 None:intergenic
ATACTCCTTGTTTGGTTGTT+TGG 0.215702 4_4:-66475656 Msa0696520:three_prime_UTR
TTAATTCTACTTTAAAATAT+TGG 0.218641 4_4:-66476776 Msa0696520:three_prime_UTR
TACTCCTTGTTTGGTTGTTT+GGG 0.231065 4_4:-66475655 Msa0696520:three_prime_UTR
CCATCATAATTGTACATTAT+AGG 0.232144 4_4:+66475936 None:intergenic
CGAGGATATAAATGGATTAA+TGG 0.243493 4_4:-66478432 Msa0696520:CDS
GTGGTCCAATATTTATTCTT+TGG 0.249478 4_4:+66476717 None:intergenic
ATTCAAGACAGTAGTTAAAT+TGG 0.250833 4_4:+66479393 None:intergenic
GACCACGAGTACCATTTAAT+TGG 0.252942 4_4:+66477551 None:intergenic
TTGTTTGGGAGCTGGGAATT+AGG 0.275658 4_4:-66475641 Msa0696520:three_prime_UTR
CTTTCTTGCAAGAAGCAAAA+TGG 0.287959 4_4:-66477038 Msa0696520:CDS
GAAGCAAAATGGTTTGCTTC+TGG 0.293280 4_4:-66477027 Msa0696520:CDS
GTCCTTGATATTCATTTCTC+TGG 0.295905 4_4:+66479038 None:intergenic
TTGTTTGGTTGTTTGGGAGC+TGG 0.298477 4_4:-66475649 Msa0696520:three_prime_UTR
CTAGAGATGAACCAATTAAA+TGG 0.303622 4_4:-66477562 Msa0696520:CDS
AGACAACACTATTGGTCTAT+TGG 0.313372 4_4:+66476458 None:intergenic
GAAACTAGAATAGGCATGTT+TGG 0.319225 4_4:+66479426 None:intergenic
TAACCAATGAATTTGCTCTT+AGG 0.320272 4_4:-66477228 Msa0696520:CDS
CTTAGGTTTGGCAAAGGACT+TGG 0.338530 4_4:-66477591 Msa0696520:CDS
TTTGGCTGTTGTAGATATAA+TGG 0.338929 4_4:+66476867 None:intergenic
AAATCCAAAGCTTATTGTAT+TGG 0.348577 4_4:-66479587 Msa0696520:five_prime_UTR
CTACAACAGCCAAAATTCTA+AGG 0.352122 4_4:-66476858 Msa0696520:CDS
ATCATCAATGATGTATATTA+GGG 0.352665 4_4:+66477466 None:intergenic
TGAGAAAACCGTGTCTCTAA+TGG 0.359133 4_4:-66476902 Msa0696520:CDS
TACAGACGAATGTTTATCTA+TGG 0.359859 4_4:-66479158 Msa0696520:CDS
GATAACCCAGGAGTTTCTAT+TGG 0.360210 4_4:-66476086 Msa0696520:three_prime_UTR
ATGACCAGTCATTAAGATAT+TGG 0.361404 4_4:+66478339 None:intergenic
TTTAACCTCATTAATAAAAT+CGG 0.373362 4_4:-66477179 Msa0696520:intron
TGTTTGGTTGTTTGGGAGCT+GGG 0.377861 4_4:-66475648 Msa0696520:three_prime_UTR
GGCACATTACTAATAATCTA+TGG 0.381390 4_4:-66479533 Msa0696520:exon
GCTTCTCAATTGAGCATAGA+AGG 0.389737 4_4:-66478398 Msa0696520:CDS
TGTGAGCTCAATCCTTTCTT+CGG 0.393162 4_4:+66477499 None:intergenic
TGAAGAAAATGTTGAATGAT+TGG 0.395622 4_4:+66479483 None:intergenic
ATGTGCCAAAGAATAAATAT+TGG 0.395885 4_4:-66476722 Msa0696520:three_prime_UTR
TAGTGTTGTCTCAGATGATT+AGG 0.397016 4_4:-66476446 Msa0696520:three_prime_UTR
ATAGTTCATGTATGTATTGC+GGG 0.400671 4_4:+66476666 None:intergenic
GAAACCAATACAATAAGCTT+TGG 0.402507 4_4:+66479583 None:intergenic
TCTTCGGAGAACTGTGGACC+TGG 0.403166 4_4:+66477515 None:intergenic
GCATAGTAAGAATTATTACT+AGG 0.406892 4_4:-66476333 Msa0696520:three_prime_UTR
CTGCAGAATGCTTTGATGTT+GGG 0.412622 4_4:+66475838 None:intergenic
GTTTGTCCAATAGAAACTCC+TGG 0.424695 4_4:+66476080 None:intergenic
GTGTACTCCAGTGCTTACTA+TGG 0.436836 4_4:+66476969 None:intergenic
GTTAATTGTGAGGGTAATGA+TGG 0.436983 4_4:-66476134 Msa0696520:three_prime_UTR
AAACTAGAATAGGCATGTTT+GGG 0.438056 4_4:+66479427 None:intergenic
GTTTGGACGCAGATGGGACT+TGG 0.438150 4_4:-66477308 Msa0696520:intron
TTAGTAAAAGATGATGGTAA+TGG 0.444587 4_4:+66475987 None:intergenic
ATCATCTGAGACAACACTAT+TGG 0.447386 4_4:+66476450 None:intergenic
TTTATTATAGAAACTAGAAT+AGG 0.448429 4_4:+66479417 None:intergenic
CAAATTCATACTCCTAAAAG+TGG 0.456424 4_4:-66479370 Msa0696520:CDS
TGCTGTATGAAGAAGATATA+TGG 0.466556 4_4:-66476368 Msa0696520:three_prime_UTR
GTGTGGATATACTCCTTGTT+TGG 0.468711 4_4:-66475664 Msa0696520:three_prime_UTR
GACTGGTCATCAACATTTCA+TGG 0.469127 4_4:-66478326 Msa0696520:CDS
CTGCATGAAAGTGTGTTTCA+AGG 0.472196 4_4:-66477263 Msa0696520:CDS
CATTAATCCATTTATATCCT+CGG 0.474307 4_4:+66478433 None:intergenic
ATTAATGAAGTTAGAAACAT+AGG 0.477000 4_4:+66476794 None:intergenic
TATAGATGGTGGAAAGACTT+AGG 0.478515 4_4:-66477608 Msa0696520:intron
GACTCGGGTTCAGTAAAATC+AGG 0.478690 4_4:+66475694 None:intergenic
TCAAACAAATCATGTATTGC+GGG 0.491332 4_4:-66476243 Msa0696520:three_prime_UTR
GTGAAATCGTTGCTTTATGA+TGG 0.495348 4_4:-66475885 Msa0696520:three_prime_UTR
AAACACACTTTCATGCAGTC+TGG 0.496127 4_4:+66477268 None:intergenic
ATGGTGGAAAGACTTAGGTT+TGG 0.496137 4_4:-66477603 Msa0696520:CDS
GTGAACAAATTTCAGATGCA+TGG 0.498506 4_4:-66476043 Msa0696520:three_prime_UTR
TGGTCAATGGCATGTCTCCC+AGG 0.498526 4_4:-66477533 Msa0696520:CDS
TCTAAGGCTATGTGATGACT+TGG 0.498674 4_4:-66476842 Msa0696520:CDS
CATAGTAAGAATTATTACTA+GGG 0.499741 4_4:-66476332 Msa0696520:three_prime_UTR
TGTCCTAAGAGCAAATTCAT+TGG 0.505116 4_4:+66477225 None:intergenic
GTCCACAGTTCTCCGAAGAA+AGG 0.506139 4_4:-66477511 Msa0696520:CDS
AGCATTGGATGAACTTAAGC+AGG 0.506378 4_4:-66479206 Msa0696520:CDS
TATAAGACATGCAAAAGCAT+TGG 0.509430 4_4:-66479221 Msa0696520:CDS
TTTCGTATGTTGAGACAAGA+AGG 0.509440 4_4:-66478995 Msa0696520:CDS
CCTATAATGTACAATTATGA+TGG 0.511514 4_4:-66475936 Msa0696520:three_prime_UTR
AGAATAGCATAGACACACTT+TGG 0.511989 4_4:-66475617 Msa0696520:three_prime_UTR
TTCCAGAGAAATGAATATCA+AGG 0.522241 4_4:-66479040 Msa0696520:CDS
ACTGCAGAATGCTTTGATGT+TGG 0.532928 4_4:+66475837 None:intergenic
CCACTCTCAAGCGAAATTTG+TGG 0.534735 4_4:-66478303 Msa0696520:CDS
GTGGATTCCATCAAAAGATT+AGG 0.535767 4_4:-66479136 Msa0696520:CDS
TTTGTCCAATAGAAACTCCT+GGG 0.537264 4_4:+66476081 None:intergenic
TATCCACACTTTATGTGACT+CGG 0.537819 4_4:+66475678 None:intergenic
AGACGAATGTTTATCTATGG+TGG 0.538797 4_4:-66479155 Msa0696520:CDS
ATCATGTATTGCGGGACGGA+GGG 0.538896 4_4:-66476235 Msa0696520:three_prime_UTR
GCTGTTGTAGATATAATGGT+TGG 0.540737 4_4:+66476871 None:intergenic
TAAATGGTACTCGTGGTCAA+TGG 0.541293 4_4:-66477546 Msa0696520:CDS
CAAAGTTTAGTAAAAGATGA+TGG 0.541713 4_4:+66475981 None:intergenic
AGATTATTAGTAATGTGCCT+TGG 0.543952 4_4:+66479537 None:intergenic
AAGAATGCCATAGTAAGCAC+TGG 0.543981 4_4:-66476976 Msa0696520:CDS
TTCCATCAAAAGATTAGGCA+TGG 0.555371 4_4:-66479131 Msa0696520:CDS
GCATAGACACACTTTGGAAG+AGG 0.555805 4_4:-66475611 Msa0696520:three_prime_UTR
TGCAGAATGCTTTGATGTTG+GGG 0.557340 4_4:+66475839 None:intergenic
ATCCACACTTTATGTGACTC+GGG 0.559082 4_4:+66475679 None:intergenic
TTGCGGGACGGAGGGAGTAG+TGG 0.560185 4_4:-66476227 Msa0696520:three_prime_UTR
GTAATAATTTAGTATGATTG+TGG 0.560611 4_4:+66476698 None:intergenic
CTGCAGGATGTTAATTGTGA+GGG 0.561151 4_4:-66476143 Msa0696520:three_prime_UTR
CTTCGGAGAACTGTGGACCT+GGG 0.567196 4_4:+66477516 None:intergenic
GAAGGGTATTACATTAGCCC+AGG 0.567931 4_4:-66478977 Msa0696520:intron
GCTGCCAATATCTTAATGAC+TGG 0.568791 4_4:-66478343 Msa0696520:CDS
TGTATGTATTGCGGGACGGA+GGG 0.569402 4_4:+66476674 None:intergenic
TTGTCTCAATGCTGCACGGA+TGG 0.569745 4_4:-66475961 Msa0696520:three_prime_UTR
TCCTAAAAGTGGAAAATGCA+AGG 0.571235 4_4:-66479359 Msa0696520:intron
CCACAAATTTCGCTTGAGAG+TGG 0.573780 4_4:+66478303 None:intergenic
AATAGTTCATGTATGTATTG+CGG 0.573838 4_4:+66476665 None:intergenic
CTTAGGACATACATCAAACA+TGG 0.577045 4_4:-66477211 Msa0696520:CDS
CTGTTGTAGATATAATGGTT+GGG 0.577593 4_4:+66476872 None:intergenic
GAAGACTGTCTTGACAATGT+AGG 0.580932 4_4:-66478374 Msa0696520:CDS
GTCAAACAAATCATGTATTG+CGG 0.583095 4_4:-66476244 Msa0696520:three_prime_UTR
AAATCGTTGCTTTATGATGG+TGG 0.583566 4_4:-66475882 Msa0696520:three_prime_UTR
AACTTTGTCTCAATGCTGCA+CGG 0.584173 4_4:-66475965 Msa0696520:three_prime_UTR
ATCCTTTCTTCGGAGAACTG+TGG 0.586049 4_4:+66477509 None:intergenic
TGGAAATGTTCCAAATTCAG+AGG 0.589748 4_4:-66477007 Msa0696520:CDS
GGACATACATCAAACATGGA+TGG 0.590806 4_4:-66477207 Msa0696520:CDS
AGCTCCCAAACAACCAAACA+AGG 0.596345 4_4:+66475651 None:intergenic
AAATTGAAGCAACACTTGAA+AGG 0.596654 4_4:-66479090 Msa0696520:CDS
AAAATTTGAGAAGGTCAAGT+GGG 0.600944 4_4:+66476626 None:intergenic
CAATACTTCTTCAAAGCTAG+AGG 0.600961 4_4:-66475913 Msa0696520:three_prime_UTR
CATCTCTCTTACTCAAACCA+AGG 0.603238 4_4:-66479554 Msa0696520:five_prime_UTR
ACTGCAGGATGTTAATTGTG+AGG 0.605083 4_4:-66476144 Msa0696520:three_prime_UTR
ATGTATGTATTGCGGGACGG+AGG 0.605733 4_4:+66476673 None:intergenic
GCATTTACTAAGATTAAACA+TGG 0.607561 4_4:+66476590 None:intergenic
AGTTTCTATTGGACAAACAA+AGG 0.608984 4_4:-66476075 Msa0696520:three_prime_UTR
GAAAGACTTAGGTTTGGCAA+AGG 0.612512 4_4:-66477597 Msa0696520:CDS
GAAACTCTTAAATCTAAGCA+TGG 0.613254 4_4:+66479060 None:intergenic
AATCATGTATTGCGGGACGG+AGG 0.617010 4_4:-66476236 Msa0696520:three_prime_UTR
TGATGACTTGGAAGGCGACA+AGG 0.619282 4_4:-66476830 Msa0696520:CDS
TCTTTACAGATGCTGTTGAG+AGG 0.626983 4_4:-66477406 Msa0696520:intron
ACAAACAAATAAAACTAACA+TGG 0.628841 4_4:-66476196 Msa0696520:three_prime_UTR
AGGCTATGTGATGACTTGGA+AGG 0.633610 4_4:-66476838 Msa0696520:CDS
AACCAATTAAATGGTACTCG+TGG 0.634167 4_4:-66477553 Msa0696520:CDS
TTCGTATGTTGAGACAAGAA+GGG 0.638397 4_4:-66478994 Msa0696520:CDS
ATGATGTATATTAGGGACAG+AGG 0.640251 4_4:+66477473 None:intergenic
ACAAATCATGTATTGCGGGA+CGG 0.640721 4_4:-66476239 Msa0696520:three_prime_UTR
TTCATGTATGTATTGCGGGA+CGG 0.643051 4_4:+66476670 None:intergenic
GAAGTCATCCATTAGAGACA+CGG 0.643126 4_4:+66476894 None:intergenic
AACCCGAGTCACATAAAGTG+TGG 0.643938 4_4:-66475681 Msa0696520:three_prime_UTR
AAAGCGTAGTTACACGTCTG+TGG 0.648801 4_4:-66476499 Msa0696520:three_prime_UTR
CAATTGAGAAGCTTCAAACA+AGG 0.656364 4_4:+66478409 None:intergenic
ATAGTAAGAATTATTACTAG+GGG 0.658427 4_4:-66476331 Msa0696520:three_prime_UTR
TGCATGGAAACAACTCAACA+AGG 0.663635 4_4:-66476027 Msa0696520:three_prime_UTR
TTAATTGTGAGGGTAATGAT+GGG 0.700457 4_4:-66476133 Msa0696520:three_prime_UTR
GACTTGGAAGGCGACAAGGT+AGG 0.702523 4_4:-66476826 Msa0696520:CDS
TGTTACATGAAAGATAACCC+AGG 0.716647 4_4:-66476098 Msa0696520:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAATTGTTTGTTT+AGG - chr4_4:66476219-66476238 Msa0696520:three_prime_UTR 10.0%
!!! TTAATTCTACTTTAAAATAT+TGG - chr4_4:66478308-66478327 Msa0696520:CDS 10.0%
!! TTTATTATAGAAACTAGAAT+AGG + chr4_4:66475670-66475689 None:intergenic 15.0%
!!! AATTTTTATGAAAGTTAAGT+AGG + chr4_4:66476978-66476997 None:intergenic 15.0%
!!! TTTTTTCAAGAAAAAACAAA+TGG - chr4_4:66477098-66477117 Msa0696520:intron 15.0%
!!! TTTTTTTTTTAATGATTTGG+AGG + chr4_4:66477300-66477319 None:intergenic 15.0%
!!! GTTTAATTTTTATACATTGT+CGG + chr4_4:66477395-66477414 None:intergenic 15.0%
!!! GATGATATATTTGATTTTTA+TGG - chr4_4:66477635-66477654 Msa0696520:intron 15.0%
!!! TTTAATTTTTTATTTGAGTG+AGG + chr4_4:66477717-66477736 None:intergenic 15.0%
!!! GATTTTTTTTTTTACTAGAA+TGG + chr4_4:66477740-66477759 None:intergenic 15.0%
!! TTTAACCTCATTAATAAAAT+CGG - chr4_4:66477905-66477924 Msa0696520:intron 15.0%
!! TACTTAATTGTGATTAATTT+GGG - chr4_4:66477988-66478007 Msa0696520:intron 15.0%
!!! TTATTTTAAATAATTACTGC+AGG - chr4_4:66478925-66478944 Msa0696520:intron 15.0%
!!! GTTGATATTTTAATAAAATG+AGG - chr4_4:66479533-66479552 Msa0696520:exon 15.0%
!!! AAACAAAAGCACAAATATTT+TGG - chr4_4:66475623-66475642 Msa0696520:three_prime_UTR 20.0%
!!! TCTTTTTCTTTAGTTTCATA+TGG - chr4_4:66475769-66475788 Msa0696520:three_prime_UTR 20.0%
!! TCCTAAATGATTAATTGAAA+GGG + chr4_4:66475834-66475853 None:intergenic 20.0%
!! ATCCTAAATGATTAATTGAA+AGG + chr4_4:66475835-66475854 None:intergenic 20.0%
!!! ATTAGATTGGTTTTTAACTT+TGG - chr4_4:66476176-66476195 Msa0696520:three_prime_UTR 20.0%
!!! TTTTTTATCAAAATGACACA+TGG - chr4_4:66477205-66477224 Msa0696520:CDS 20.0%
!!! ATATGTATGACTTTAAAAGT+AGG + chr4_4:66477338-66477357 None:intergenic 20.0%
!! TGATGTAATCATTAGTAATA+CGG + chr4_4:66477441-66477460 None:intergenic 20.0%
!! GATTACATCATTTATAATCA+TGG - chr4_4:66477451-66477470 Msa0696520:CDS 20.0%
!! TTATAATCATGGTATATAGA+TGG - chr4_4:66477462-66477481 Msa0696520:CDS 20.0%
!! ATCATCAATGATGTATATTA+GGG + chr4_4:66477621-66477640 None:intergenic 20.0%
!! TATCATCAATGATGTATATT+AGG + chr4_4:66477622-66477641 None:intergenic 20.0%
!!! GATTTTATTAATGAGGTTAA+AGG + chr4_4:66477906-66477925 None:intergenic 20.0%
!!! CTATAAAGATTTAGAATGAA+GGG + chr4_4:66477935-66477954 None:intergenic 20.0%
!!! ACTATAAAGATTTAGAATGA+AGG + chr4_4:66477936-66477955 None:intergenic 20.0%
!! CTACTTAATTGTGATTAATT+TGG - chr4_4:66477987-66478006 Msa0696520:intron 20.0%
!! ATTAATGAAGTTAGAAACAT+AGG + chr4_4:66478293-66478312 None:intergenic 20.0%
!!! ATTTTTCTAGGGAATTTTTT+TGG + chr4_4:66478344-66478363 None:intergenic 20.0%
!! GTAATAATTTAGTATGATTG+TGG + chr4_4:66478389-66478408 None:intergenic 20.0%
!! CATAGTAAGAATTATTACTA+GGG - chr4_4:66478752-66478771 Msa0696520:intron 20.0%
!! ATAGTAAGAATTATTACTAG+GGG - chr4_4:66478753-66478772 Msa0696520:intron 20.0%
!!! TTTTCTTAATCTGTATGAAA+CGG - chr4_4:66478818-66478837 Msa0696520:intron 20.0%
!! ACAAACAAATAAAACTAACA+TGG - chr4_4:66478888-66478907 Msa0696520:intron 20.0%
!!! TTTTTGATACATTTTGAGTT+AGG - chr4_4:66479597-66479616 Msa0696520:five_prime_UTR 20.0%
! AAATCCAAAGCTTATTGTAT+TGG - chr4_4:66475497-66475516 Msa0696520:three_prime_UTR 25.0%
! TGAAGAAAATGTTGAATGAT+TGG + chr4_4:66475604-66475623 None:intergenic 25.0%
! ATTCAAGACAGTAGTTAAAT+TGG + chr4_4:66475694-66475713 None:intergenic 25.0%
! CTTAATTAGCTACAAGTTAA+TGG + chr4_4:66475807-66475826 None:intergenic 25.0%
! ACCCTTTCAATTAATCATTT+AGG - chr4_4:66475830-66475849 Msa0696520:three_prime_UTR 25.0%
! TTGAGTATAGAAAATTTGTG+AGG + chr4_4:66476332-66476351 None:intergenic 25.0%
!! TTTAGGATATCAACAATGTT+AGG + chr4_4:66476416-66476435 None:intergenic 25.0%
! CTTAGATAGGTATATGTAAT+TGG + chr4_4:66476524-66476543 None:intergenic 25.0%
! CATTAATCCATTTATATCCT+CGG + chr4_4:66476654-66476673 None:intergenic 25.0%
!!! CTTGACAAAGTTTTATGAAT+TGG + chr4_4:66476824-66476843 None:intergenic 25.0%
!! TTTTGTGATTGCATTATTGT+TGG + chr4_4:66476854-66476873 None:intergenic 25.0%
!!! GTACGTTTTTAAAAGATTCA+AGG - chr4_4:66476999-66477018 Msa0696520:CDS 25.0%
! GTATTTGATTCATTAACATG+TGG + chr4_4:66477025-66477044 None:intergenic 25.0%
! TTTCAAGAAAAAACAAATGG+CGG - chr4_4:66477101-66477120 Msa0696520:intron 25.0%
!! CTTAATAGCATTCTTTTGTT+TGG - chr4_4:66477759-66477778 Msa0696520:intron 25.0%
!!! GAATACCGATTTTATTAATG+AGG + chr4_4:66477913-66477932 None:intergenic 25.0%
! ATGTGCCAAAGAATAAATAT+TGG - chr4_4:66478362-66478381 Msa0696520:CDS 25.0%
! AATAGTTCATGTATGTATTG+CGG + chr4_4:66478422-66478441 None:intergenic 25.0%
! ATCTGCTTAAAAATTTGAGA+AGG + chr4_4:66478470-66478489 None:intergenic 25.0%
! GCATTTACTAAGATTAAACA+TGG + chr4_4:66478497-66478516 None:intergenic 25.0%
!! ATGCTTTTGTAAAACTTTTG+TGG - chr4_4:66478513-66478532 Msa0696520:intron 25.0%
!!! CTTACATACACTTTTTACAT+GGG + chr4_4:66478540-66478559 None:intergenic 25.0%
!!! TCTTACATACACTTTTTACA+TGG + chr4_4:66478541-66478560 None:intergenic 25.0%
!!! TATTCACGTTTTTCAATTCA+TGG + chr4_4:66478694-66478713 None:intergenic 25.0%
! GCATAGTAAGAATTATTACT+AGG - chr4_4:66478751-66478770 Msa0696520:intron 25.0%
!! TTAGTAAAAGATGATGGTAA+TGG + chr4_4:66479100-66479119 None:intergenic 25.0%
! CAAAGTTTAGTAAAAGATGA+TGG + chr4_4:66479106-66479125 None:intergenic 25.0%
! CCATCATAATTGTACATTAT+AGG + chr4_4:66479151-66479170 None:intergenic 25.0%
! CCTATAATGTACAATTATGA+TGG - chr4_4:66479148-66479167 Msa0696520:CDS 25.0%
GAAACCAATACAATAAGCTT+TGG + chr4_4:66475504-66475523 None:intergenic 30.0%
AGATTATTAGTAATGTGCCT+TGG + chr4_4:66475550-66475569 None:intergenic 30.0%
GGCACATTACTAATAATCTA+TGG - chr4_4:66475551-66475570 Msa0696520:three_prime_UTR 30.0%
AAACTAGAATAGGCATGTTT+GGG + chr4_4:66475660-66475679 None:intergenic 30.0%
CAAATTCATACTCCTAAAAG+TGG - chr4_4:66475714-66475733 Msa0696520:three_prime_UTR 30.0%
! TATAAGACATGCAAAAGCAT+TGG - chr4_4:66475863-66475882 Msa0696520:three_prime_UTR 30.0%
TACAGACGAATGTTTATCTA+TGG - chr4_4:66475926-66475945 Msa0696520:three_prime_UTR 30.0%
AAATTGAAGCAACACTTGAA+AGG - chr4_4:66475994-66476013 Msa0696520:three_prime_UTR 30.0%
GAAACTCTTAAATCTAAGCA+TGG + chr4_4:66476027-66476046 None:intergenic 30.0%
TTCCAGAGAAATGAATATCA+AGG - chr4_4:66476044-66476063 Msa0696520:three_prime_UTR 30.0%
CCAATCTAATCAATGTAGAT+TGG + chr4_4:66476166-66476185 None:intergenic 30.0%
! CCAATCTACATTGATTAGAT+TGG - chr4_4:66476163-66476182 Msa0696520:three_prime_UTR 30.0%
AAAAACAAGGTCATTGTCAT+GGG + chr4_4:66476564-66476583 None:intergenic 30.0%
AAAAAACAAGGTCATTGTCA+TGG + chr4_4:66476565-66476584 None:intergenic 30.0%
!! GATAAGTTTTGTGACAACAA+AGG - chr4_4:66476608-66476627 Msa0696520:three_prime_UTR 30.0%
! ACATTTTCCGAGGATATAAA+TGG - chr4_4:66476644-66476663 Msa0696520:three_prime_UTR 30.0%
!! CGAGGATATAAATGGATTAA+TGG - chr4_4:66476652-66476671 Msa0696520:three_prime_UTR 30.0%
ATGACCAGTCATTAAGATAT+TGG + chr4_4:66476748-66476767 None:intergenic 30.0%
TTTATGAATTGGATACGTCA+AGG + chr4_4:66476813-66476832 None:intergenic 30.0%
TTCAGAAATAGACACTCAAA+TGG - chr4_4:66476904-66476923 Msa0696520:CDS 30.0%
TAATCATGGTATATAGATGG+TGG - chr4_4:66477465-66477484 Msa0696520:CDS 30.0%
CTAGAGATGAACCAATTAAA+TGG - chr4_4:66477522-66477541 Msa0696520:CDS 30.0%
! TAACCAATGAATTTGCTCTT+AGG - chr4_4:66477856-66477875 Msa0696520:intron 30.0%
GAAGATAGTATGTTACACAT+AGG + chr4_4:66477968-66477987 None:intergenic 30.0%
TGGGTGTTAATTAATTACAG+TGG - chr4_4:66478007-66478026 Msa0696520:intron 30.0%
GGGTGTTAATTAATTACAGT+GGG - chr4_4:66478008-66478027 Msa0696520:intron 30.0%
TTCAAATACTCCTCTGAATT+TGG + chr4_4:66478090-66478109 None:intergenic 30.0%
CTGTTGTAGATATAATGGTT+GGG + chr4_4:66478215-66478234 None:intergenic 30.0%
TTTGGCTGTTGTAGATATAA+TGG + chr4_4:66478220-66478239 None:intergenic 30.0%
!! ATCACATAGCCTTAGAATTT+TGG + chr4_4:66478238-66478257 None:intergenic 30.0%
! TTCTTTGGCACATTTTTCTA+GGG + chr4_4:66478355-66478374 None:intergenic 30.0%
! ATTCTTTGGCACATTTTTCT+AGG + chr4_4:66478356-66478375 None:intergenic 30.0%
GTGGTCCAATATTTATTCTT+TGG + chr4_4:66478370-66478389 None:intergenic 30.0%
ATAGTTCATGTATGTATTGC+GGG + chr4_4:66478421-66478440 None:intergenic 30.0%
AAAATTTGAGAAGGTCAAGT+GGG + chr4_4:66478461-66478480 None:intergenic 30.0%
AAAAATTTGAGAAGGTCAAG+TGG + chr4_4:66478462-66478481 None:intergenic 30.0%
TGCTGTATGAAGAAGATATA+TGG - chr4_4:66478716-66478735 Msa0696520:intron 30.0%
GTCAAACAAATCATGTATTG+CGG - chr4_4:66478840-66478859 Msa0696520:intron 30.0%
TCAAACAAATCATGTATTGC+GGG - chr4_4:66478841-66478860 Msa0696520:intron 30.0%
TTAATTGTGAGGGTAATGAT+GGG - chr4_4:66478951-66478970 Msa0696520:intron 30.0%
AGTTTCTATTGGACAAACAA+AGG - chr4_4:66479009-66479028 Msa0696520:CDS 30.0%
GAAACTAGAATAGGCATGTT+TGG + chr4_4:66475661-66475680 None:intergenic 35.0%
TCCTAAAAGTGGAAAATGCA+AGG - chr4_4:66475725-66475744 Msa0696520:three_prime_UTR 35.0%
!! ACCTTGCATTTTCCACTTTT+AGG + chr4_4:66475729-66475748 None:intergenic 35.0%
AGACGAATGTTTATCTATGG+TGG - chr4_4:66475929-66475948 Msa0696520:three_prime_UTR 35.0%
GTGGATTCCATCAAAAGATT+AGG - chr4_4:66475948-66475967 Msa0696520:three_prime_UTR 35.0%
TTCCATCAAAAGATTAGGCA+TGG - chr4_4:66475953-66475972 Msa0696520:three_prime_UTR 35.0%
!! CATGGAGTACAATTTTGAAG+AGG - chr4_4:66475971-66475990 Msa0696520:three_prime_UTR 35.0%
GTCCTTGATATTCATTTCTC+TGG + chr4_4:66476049-66476068 None:intergenic 35.0%
TTTCGTATGTTGAGACAAGA+AGG - chr4_4:66476089-66476108 Msa0696520:three_prime_UTR 35.0%
TTCGTATGTTGAGACAAGAA+GGG - chr4_4:66476090-66476109 Msa0696520:three_prime_UTR 35.0%
ACTTGAAAGCAAACTAACCT+GGG + chr4_4:66476127-66476146 None:intergenic 35.0%
TACTTGAAAGCAAACTAACC+TGG + chr4_4:66476128-66476147 None:intergenic 35.0%
!! AGGTTAGTTTGCTTTCAAGT+AGG - chr4_4:66476127-66476146 Msa0696520:three_prime_UTR 35.0%
TTGTCTAATAGAAACCCATG+TGG - chr4_4:66476360-66476379 Msa0696520:three_prime_UTR 35.0%
!!! AGTGCTGTGTGACTTTTTTT+AGG - chr4_4:66476385-66476404 Msa0696520:three_prime_UTR 35.0%
ACATCATGCATCACTTAGAT+AGG + chr4_4:66476537-66476556 None:intergenic 35.0%
AAAACAAGGTCATTGTCATG+GGG + chr4_4:66476563-66476582 None:intergenic 35.0%
TAAGATGCAGCGAAAAAACA+AGG + chr4_4:66476577-66476596 None:intergenic 35.0%
CAATTGAGAAGCTTCAAACA+AGG + chr4_4:66476678-66476697 None:intergenic 35.0%
AGTTAAGTAGGACTCTTACT+TGG + chr4_4:66476966-66476985 None:intergenic 35.0%
!! TATAGATGGTGGAAAGACTT+AGG - chr4_4:66477476-66477495 Msa0696520:CDS 35.0%
AACCAATTAAATGGTACTCG+TGG - chr4_4:66477531-66477550 Msa0696520:CDS 35.0%
ATGATGTATATTAGGGACAG+AGG + chr4_4:66477614-66477633 None:intergenic 35.0%
TGTCCTAAGAGCAAATTCAT+TGG + chr4_4:66477862-66477881 None:intergenic 35.0%
CTTAGGACATACATCAAACA+TGG - chr4_4:66477873-66477892 Msa0696520:intron 35.0%
CTTTCTTGCAAGAAGCAAAA+TGG - chr4_4:66478046-66478065 Msa0696520:intron 35.0%
TGGAAATGTTCCAAATTCAG+AGG - chr4_4:66478077-66478096 Msa0696520:intron 35.0%
GCTGTTGTAGATATAATGGT+TGG + chr4_4:66478216-66478235 None:intergenic 35.0%
CTACAACAGCCAAAATTCTA+AGG - chr4_4:66478226-66478245 Msa0696520:intron 35.0%
!!! GGGAATTTTTTTGGTACATG+TGG + chr4_4:66478335-66478354 None:intergenic 35.0%
! AGACAACACTATTGGTCTAT+TGG + chr4_4:66478629-66478648 None:intergenic 35.0%
! ATCATCTGAGACAACACTAT+TGG + chr4_4:66478637-66478656 None:intergenic 35.0%
TAGTGTTGTCTCAGATGATT+AGG - chr4_4:66478638-66478657 Msa0696520:intron 35.0%
GTTAATTGTGAGGGTAATGA+TGG - chr4_4:66478950-66478969 Msa0696520:intron 35.0%
TGTTACATGAAAGATAACCC+AGG - chr4_4:66478986-66479005 Msa0696520:CDS 35.0%
TTTGTCCAATAGAAACTCCT+GGG + chr4_4:66479006-66479025 None:intergenic 35.0%
GTGAACAAATTTCAGATGCA+TGG - chr4_4:66479041-66479060 Msa0696520:CDS 35.0%
CAATACTTCTTCAAAGCTAG+AGG - chr4_4:66479171-66479190 Msa0696520:CDS 35.0%
! GTGAAATCGTTGCTTTATGA+TGG - chr4_4:66479199-66479218 Msa0696520:CDS 35.0%
! AAATCGTTGCTTTATGATGG+TGG - chr4_4:66479202-66479221 Msa0696520:CDS 35.0%
TATCCACACTTTATGTGACT+CGG + chr4_4:66479409-66479428 None:intergenic 35.0%
! ATACTCCTTGTTTGGTTGTT+TGG - chr4_4:66479428-66479447 Msa0696520:CDS 35.0%
! TACTCCTTGTTTGGTTGTTT+GGG - chr4_4:66479429-66479448 Msa0696520:CDS 35.0%
AGAATAGCATAGACACACTT+TGG - chr4_4:66479467-66479486 Msa0696520:CDS 35.0%
CATCTCTCTTACTCAAACCA+AGG - chr4_4:66475530-66475549 Msa0696520:three_prime_UTR 40.0%
! AGCATTGGATGAACTTAAGC+AGG - chr4_4:66475878-66475897 Msa0696520:three_prime_UTR 40.0%
! CTCCATGCCTAATCTTTTGA+TGG + chr4_4:66475958-66475977 None:intergenic 40.0%
! ACTCAAGCATACATTTTCCG+AGG - chr4_4:66476634-66476653 Msa0696520:three_prime_UTR 40.0%
GCTTCTCAATTGAGCATAGA+AGG - chr4_4:66476686-66476705 Msa0696520:three_prime_UTR 40.0%
GAAGACTGTCTTGACAATGT+AGG - chr4_4:66476710-66476729 Msa0696520:three_prime_UTR 40.0%
GCTGCCAATATCTTAATGAC+TGG - chr4_4:66476741-66476760 Msa0696520:three_prime_UTR 40.0%
! GACTGGTCATCAACATTTCA+TGG - chr4_4:66476758-66476777 Msa0696520:three_prime_UTR 40.0%
TGCAATCACAAAATGCAGCA+TGG - chr4_4:66476861-66476880 Msa0696520:CDS 40.0%
GGTTTCATCATCACATCTCA+AGG - chr4_4:66476925-66476944 Msa0696520:CDS 40.0%
! ATGGTGGAAAGACTTAGGTT+TGG - chr4_4:66477481-66477500 Msa0696520:CDS 40.0%
GAAAGACTTAGGTTTGGCAA+AGG - chr4_4:66477487-66477506 Msa0696520:CDS 40.0%
GACCACGAGTACCATTTAAT+TGG + chr4_4:66477536-66477555 None:intergenic 40.0%
TAAATGGTACTCGTGGTCAA+TGG - chr4_4:66477538-66477557 Msa0696520:CDS 40.0%
TGTGAGCTCAATCCTTTCTT+CGG + chr4_4:66477588-66477607 None:intergenic 40.0%
TCTTTACAGATGCTGTTGAG+AGG - chr4_4:66477678-66477697 Msa0696520:intron 40.0%
!! TTCTTTTGTTTGGACGCAGA+TGG - chr4_4:66477769-66477788 Msa0696520:intron 40.0%
!! TCTTTTGTTTGGACGCAGAT+GGG - chr4_4:66477770-66477789 Msa0696520:intron 40.0%
AAACACACTTTCATGCAGTC+TGG + chr4_4:66477819-66477838 None:intergenic 40.0%
! CTGCATGAAAGTGTGTTTCA+AGG - chr4_4:66477821-66477840 Msa0696520:intron 40.0%
GGACATACATCAAACATGGA+TGG - chr4_4:66477877-66477896 Msa0696520:intron 40.0%
!! GAAGCAAAATGGTTTGCTTC+TGG - chr4_4:66478057-66478076 Msa0696520:intron 40.0%
AAGAATGCCATAGTAAGCAC+TGG - chr4_4:66478108-66478127 Msa0696520:intron 40.0%
! TGTGCATGCTTTCTTTTGCA+TGG - chr4_4:66478146-66478165 Msa0696520:intron 40.0%
! GTGCATGCTTTCTTTTGCAT+GGG - chr4_4:66478147-66478166 Msa0696520:intron 40.0%
TGAGAAAACCGTGTCTCTAA+TGG - chr4_4:66478182-66478201 Msa0696520:intron 40.0%
GAAGTCATCCATTAGAGACA+CGG + chr4_4:66478193-66478212 None:intergenic 40.0%
! TCTAAGGCTATGTGATGACT+TGG - chr4_4:66478242-66478261 Msa0696520:intron 40.0%
TTCATGTATGTATTGCGGGA+CGG + chr4_4:66478417-66478436 None:intergenic 40.0%
ACAAATCATGTATTGCGGGA+CGG - chr4_4:66478845-66478864 Msa0696520:intron 40.0%
ACTGCAGGATGTTAATTGTG+AGG - chr4_4:66478940-66478959 Msa0696520:intron 40.0%
CTGCAGGATGTTAATTGTGA+GGG - chr4_4:66478941-66478960 Msa0696520:intron 40.0%
GATAACCCAGGAGTTTCTAT+TGG - chr4_4:66478998-66479017 Msa0696520:CDS 40.0%
GTTTGTCCAATAGAAACTCC+TGG + chr4_4:66479007-66479026 None:intergenic 40.0%
TGCATGGAAACAACTCAACA+AGG - chr4_4:66479057-66479076 Msa0696520:CDS 40.0%
AACTTTGTCTCAATGCTGCA+CGG - chr4_4:66479119-66479138 Msa0696520:CDS 40.0%
TGCAGAATGCTTTGATGTTG+GGG + chr4_4:66479248-66479267 None:intergenic 40.0%
CTGCAGAATGCTTTGATGTT+GGG + chr4_4:66479249-66479268 None:intergenic 40.0%
ACTGCAGAATGCTTTGATGT+TGG + chr4_4:66479250-66479269 None:intergenic 40.0%
ATCCACACTTTATGTGACTC+GGG + chr4_4:66479408-66479427 None:intergenic 40.0%
GTGTGGATATACTCCTTGTT+TGG - chr4_4:66479420-66479439 Msa0696520:CDS 40.0%
!! GAAGGGTATTACATTAGCCC+AGG - chr4_4:66476107-66476126 Msa0696520:three_prime_UTR 45.0%
CCACAAATTTCGCTTGAGAG+TGG + chr4_4:66476784-66476803 None:intergenic 45.0%
CCACTCTCAAGCGAAATTTG+TGG - chr4_4:66476781-66476800 Msa0696520:three_prime_UTR 45.0%
! GGAGCTCTAGTGTATCAAAC+TGG - chr4_4:66477151-66477170 Msa0696520:intron 45.0%
CTTAGGTTTGGCAAAGGACT+TGG - chr4_4:66477493-66477512 Msa0696520:CDS 45.0%
ATCCTTTCTTCGGAGAACTG+TGG + chr4_4:66477578-66477597 None:intergenic 45.0%
GTGTACTCCAGTGCTTACTA+TGG + chr4_4:66478118-66478137 None:intergenic 45.0%
! GCTTTCTTTTGCATGGGTCA+AGG - chr4_4:66478153-66478172 Msa0696520:intron 45.0%
! AGGCTATGTGATGACTTGGA+AGG - chr4_4:66478246-66478265 Msa0696520:intron 45.0%
AAAGCGTAGTTACACGTCTG+TGG - chr4_4:66478585-66478604 Msa0696520:intron 45.0%
GACTCGGGTTCAGTAAAATC+AGG + chr4_4:66479393-66479412 None:intergenic 45.0%
AACCCGAGTCACATAAAGTG+TGG - chr4_4:66479403-66479422 Msa0696520:CDS 45.0%
AGCTCCCAAACAACCAAACA+AGG + chr4_4:66479436-66479455 None:intergenic 45.0%
TTGTTTGGTTGTTTGGGAGC+TGG - chr4_4:66479435-66479454 Msa0696520:CDS 45.0%
TGTTTGGTTGTTTGGGAGCT+GGG - chr4_4:66479436-66479455 Msa0696520:CDS 45.0%
TTGTTTGGGAGCTGGGAATT+AGG - chr4_4:66479443-66479462 Msa0696520:CDS 45.0%
! GCATAGACACACTTTGGAAG+AGG - chr4_4:66479473-66479492 Msa0696520:CDS 45.0%
!!! TTTTAAGTTATTAATATTTT+AGG + chr4_4:66476433-66476452 None:intergenic 5.0%
!!! TAAAAAATAGAAATTTTTTT+TGG + chr4_4:66477177-66477196 None:intergenic 5.0%
!!! TAATTTTTTTTTTAATGATT+TGG + chr4_4:66477303-66477322 None:intergenic 5.0%
TCACACAGCACTTGCCACAT+GGG + chr4_4:66476377-66476396 None:intergenic 50.0%
GTCCACAGTTCTCCGAAGAA+AGG - chr4_4:66477573-66477592 Msa0696520:CDS 50.0%
! TGATGACTTGGAAGGCGACA+AGG - chr4_4:66478254-66478273 Msa0696520:intron 50.0%
TGTATGTATTGCGGGACGGA+GGG + chr4_4:66478413-66478432 None:intergenic 50.0%
ATGTATGTATTGCGGGACGG+AGG + chr4_4:66478414-66478433 None:intergenic 50.0%
AATCATGTATTGCGGGACGG+AGG - chr4_4:66478848-66478867 Msa0696520:intron 50.0%
ATCATGTATTGCGGGACGGA+GGG - chr4_4:66478849-66478868 Msa0696520:intron 50.0%
TTGTCTCAATGCTGCACGGA+TGG - chr4_4:66479123-66479142 Msa0696520:CDS 50.0%
GTCACACAGCACTTGCCACA+TGG + chr4_4:66476378-66476397 None:intergenic 55.0%
TGGTCAATGGCATGTCTCCC+AGG - chr4_4:66477551-66477570 Msa0696520:CDS 55.0%
CTTCGGAGAACTGTGGACCT+GGG + chr4_4:66477571-66477590 None:intergenic 55.0%
TCTTCGGAGAACTGTGGACC+TGG + chr4_4:66477572-66477591 None:intergenic 55.0%
GTTTGGACGCAGATGGGACT+TGG - chr4_4:66477776-66477795 Msa0696520:intron 55.0%
GACTTGGAAGGCGACAAGGT+AGG - chr4_4:66478258-66478277 Msa0696520:intron 55.0%
! CAAATGGCGGAACCCCTTCC+CGG - chr4_4:66477114-66477133 Msa0696520:intron 60.0%
CTAGAGCTCCCGCATACGCA+GGG + chr4_4:66477141-66477160 None:intergenic 60.0%
ACTAGAGCTCCCGCATACGC+AGG + chr4_4:66477142-66477161 None:intergenic 60.0%
CATACGCAGGGTCCGGGAAG+GGG + chr4_4:66477129-66477148 None:intergenic 65.0%
GCATACGCAGGGTCCGGGAA+GGG + chr4_4:66477130-66477149 None:intergenic 65.0%
CTTCCCGGACCCTGCGTATG+CGG - chr4_4:66477129-66477148 Msa0696520:intron 65.0%
TTCCCGGACCCTGCGTATGC+GGG - chr4_4:66477130-66477149 Msa0696520:intron 65.0%
TTGCGGGACGGAGGGAGTAG+TGG - chr4_4:66478857-66478876 Msa0696520:intron 65.0%
CGCATACGCAGGGTCCGGGA+AGG + chr4_4:66477131-66477150 None:intergenic 70.0%
CTCCCGCATACGCAGGGTCC+GGG + chr4_4:66477135-66477154 None:intergenic 70.0%
GCTCCCGCATACGCAGGGTC+CGG + chr4_4:66477136-66477155 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 66475470 66479636 66475470 ID=Msa0696520;Name=Msa0696520
chr4_4 mRNA 66475470 66479636 66475470 ID=Msa0696520-mRNA-1;Parent=Msa0696520;Name=Msa0696520-mRNA-1;_AED=0.05;_eAED=0.05;_QI=100|0.8|0.83|1|1|1|6|1334|434
chr4_4 exon 66475470 66477079 66475470 ID=Msa0696520-mRNA-1:exon:19295;Parent=Msa0696520-mRNA-1
chr4_4 exon 66477180 66477318 66477180 ID=Msa0696520-mRNA-1:exon:19294;Parent=Msa0696520-mRNA-1
chr4_4 exon 66477407 66477625 66477407 ID=Msa0696520-mRNA-1:exon:19293;Parent=Msa0696520-mRNA-1
chr4_4 exon 66478290 66478506 66478290 ID=Msa0696520-mRNA-1:exon:19292;Parent=Msa0696520-mRNA-1
chr4_4 exon 66478978 66479254 66478978 ID=Msa0696520-mRNA-1:exon:19291;Parent=Msa0696520-mRNA-1
chr4_4 exon 66479360 66479636 66479360 ID=Msa0696520-mRNA-1:exon:19290;Parent=Msa0696520-mRNA-1
chr4_4 five_prime_UTR 66479537 66479636 66479537 ID=Msa0696520-mRNA-1:five_prime_utr;Parent=Msa0696520-mRNA-1
chr4_4 CDS 66479360 66479536 66479360 ID=Msa0696520-mRNA-1:cds;Parent=Msa0696520-mRNA-1
chr4_4 CDS 66478978 66479254 66478978 ID=Msa0696520-mRNA-1:cds;Parent=Msa0696520-mRNA-1
chr4_4 CDS 66478290 66478506 66478290 ID=Msa0696520-mRNA-1:cds;Parent=Msa0696520-mRNA-1
chr4_4 CDS 66477407 66477625 66477407 ID=Msa0696520-mRNA-1:cds;Parent=Msa0696520-mRNA-1
chr4_4 CDS 66477180 66477318 66477180 ID=Msa0696520-mRNA-1:cds;Parent=Msa0696520-mRNA-1
chr4_4 CDS 66476804 66477079 66476804 ID=Msa0696520-mRNA-1:cds;Parent=Msa0696520-mRNA-1
chr4_4 three_prime_UTR 66475470 66476803 66475470 ID=Msa0696520-mRNA-1:three_prime_utr;Parent=Msa0696520-mRNA-1
Gene Sequence

>Msa0696520

ATGGCTTTTTCTCTTTCTTTCGCTGCTTCAACCAATCATTCAACATTTTCTTCAAACAAAAGCACAAATATTTTGGATATATCTATACCCAAACATGCCTATTCTAGTTTCTATAATAAAACCAATTTAACTACTGTCTTGAATCAAATTCATACTCCTAAAAGTGGAAAATGCAAGGATGATCTTCATATAAGACATGCAAAAGCATTGGATGAACTTAAGCAGGTATTTGTTAGAGATATAAGAAAAAATACAGACGAATGTTTATCTATGGTGGATTCCATCAAAAGATTAGGCATGGAGTACAATTTTGAAGAGGAAATTGAAGCAACACTTGAAAGGAAACACACCATGCTTAGATTTAAGAGTTTCCAGAGAAATGAATATCAAGGACTGTCACAAGCTGCATTTCAATTTCGTATGTTGAGACAAGAAGGGTATTACATTAGCCCAGACATATTTGATAAGTTTTGTGACAACAAAGGAAAACTCAAGCATACATTTTCCGAGGATATAAATGGATTAATGGCCTTGTTTGAAGCTTCTCAATTGAGCATAGAAGGAGAAGACTGTCTTGACAATGTAGGACAGTTCTGCTGCCAATATCTTAATGACTGGTCATCAACATTTCATGGCCACTCTCAAGCGAAATTTGTGGCACACACCTTGACATGGTGGAAAGACTTAGGTTTGGCAAAGGACTTGGAATTTGCTAGAGATGAACCAATTAAATGGTACTCGTGGTCAATGGCATGTCTCCCAGGTCCACAGTTCTCCGAAGAAAGGATTGAGCTCACAAAACCTCTGTCCCTAATATACATCATTGATGATATATTTGATTTTTATGGAAACATTGAAGAACTTACTCTCTTTACAGATGCTGTTGAGAGATGGGACTTGGCAGCTATTGAACAATTACCAGACTGCATGAAAGTGTGTTTCAAGGCTCTCTATGACATAACCAATGAATTTGCTCTTAGGACATACATCAAACATGGATGGAATCCTTTAACCTCATTAATAAAATCGTGGGTAAGACTCATGAATGCTTTCTTGCAAGAAGCAAAATGGTTTGCTTCTGGAAATGTTCCAAATTCAGAGGAGTATTTGAAGAATGCCATAGTAAGCACTGGAGTACACGTGATACTTGTGCATGCTTTCTTTTGCATGGGTCAAGGTATAACTGAGAAAACCGTGTCTCTAATGGATGACTTCCCAACCATTATATCTACAACAGCCAAAATTCTAAGGCTATGTGATGACTTGGAAGGCGACAAGGTAGGAAAACATCACCTATGTTTCTAA
Protein sequence

>Msa0696520

MAFSLSFAASTNHSTFSSNKSTNILDISIPKHAYSSFYNKTNLTTVLNQIHTPKSGKCKDDLHIRHAKALDELKQVFVRDIRKNTDECLSMVDSIKRLGMEYNFEEEIEATLERKHTMLRFKSFQRNEYQGLSQAAFQFRMLRQEGYYISPDIFDKFCDNKGKLKHTFSEDINGLMALFEASQLSIEGEDCLDNVGQFCCQYLNDWSSTFHGHSQAKFVAHTLTWWKDLGLAKDLEFARDEPIKWYSWSMACLPGPQFSEERIELTKPLSLIYIIDDIFDFYGNIEELTLFTDAVERWDLAAIEQLPDCMKVCFKALYDITNEFALRTYIKHGWNPLTSLIKSWVRLMNAFLQEAKWFASGNVPNSEEYLKNAIVSTGVHVILVHAFFCMGQGITEKTVSLMDDFPTIISTTAKILRLCDDLEGDKVGKHHLCF*