AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0559010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0559010 MtrunA17_Chr4g0048911 92.743 565 30 1 1 565 1 554 0.0 1101
Msa0559010 MtrunA17_Chr3g0109031 47.305 501 256 5 73 565 12 512 2.89e-165 480
Msa0559010 MtrunA17_Chr6g0477111 48.148 486 245 4 87 565 2 487 4.09e-164 476
Msa0559010 MtrunA17_Chr3g0109021 47.705 501 254 6 73 565 12 512 5.38e-164 477
Msa0559010 MtrunA17_Chr2g0280581 46.019 515 264 8 63 565 5 517 6.41e-164 476
Msa0559010 MtrunA17_Chr3g0105691 46.906 501 258 5 73 565 45 545 2.98e-163 476
Msa0559010 MtrunA17_Chr3g0109011 48.148 486 245 4 87 565 2 487 1.21e-162 472
Msa0559010 MtrunA17_Chr3g0108941 47.809 502 253 7 73 565 12 513 1.50e-162 473
Msa0559010 MtrunA17_Chr3g0109001 46.887 514 263 7 60 565 2 513 3.34e-162 472
Msa0559010 MtrunA17_Chr3g0108981 48.049 487 245 5 87 565 2 488 4.53e-162 471
Msa0559010 MtrunA17_Chr3g0108961 48.049 487 245 5 87 565 2 488 4.53e-162 471
Msa0559010 MtrunA17_Chr3g0108991 46.906 501 258 6 73 565 12 512 5.69e-162 471
Msa0559010 MtrunA17_Chr3g0108951 46.906 501 258 6 73 565 12 512 5.69e-162 471
Msa0559010 MtrunA17_Chr3g0099031 46.589 513 265 5 60 565 2 512 2.83e-161 469
Msa0559010 MtrunA17_Chr3g0108971 47.531 486 248 4 87 565 2 487 3.34e-161 469
Msa0559010 MtrunA17_Chr4g0048921 45.759 507 262 7 66 565 65 565 2.10e-149 441
Msa0559010 MtrunA17_Chr6g0477121 49.633 409 200 3 152 554 10 418 3.55e-143 420
Msa0559010 MtrunA17_Chr2g0323151 35.062 482 297 6 89 565 4 474 3.97e-104 322
Msa0559010 MtrunA17_Chr2g0323131 34.783 483 298 7 89 565 88 559 2.32e-103 323
Msa0559010 MtrunA17_Chr4g0023911 35.282 496 311 8 65 554 71 562 2.00e-93 297
Msa0559010 MtrunA17_Chr4g0042111 33.402 488 313 9 71 552 58 539 2.19e-90 289
Msa0559010 MtrunA17_Chr2g0319051 31.213 503 334 8 71 565 10 508 9.26e-81 262
Msa0559010 MtrunA17_Chr8g0357411 31.683 505 330 9 55 553 74 569 8.54e-80 262
Msa0559010 MtrunA17_Chr6g0465441 30.636 519 338 9 61 565 37 547 1.49e-79 260
Msa0559010 MtrunA17_Chr4g0024691 29.400 500 343 6 72 565 60 555 2.48e-79 259
Msa0559010 MtrunA17_Chr2g0310331 33.069 505 322 8 55 553 72 566 2.72e-79 260
Msa0559010 MtrunA17_Chr6g0451711 28.571 504 343 6 72 565 55 551 8.77e-78 256
Msa0559010 MtrunA17_Chr6g0465851 30.408 490 322 8 87 565 31 512 1.04e-77 254
Msa0559010 MtrunA17_Chr6g0465911 30.408 490 322 8 87 565 66 547 1.87e-77 254
Msa0559010 MtrunA17_Chr3g0082691 30.522 498 334 6 78 565 52 547 2.03e-77 254
Msa0559010 MtrunA17_Chr5g0431571 30.909 495 322 7 83 565 57 543 3.93e-77 253
Msa0559010 MtrunA17_Chr3g0105071 29.774 487 326 6 89 565 71 551 9.43e-77 253
Msa0559010 MtrunA17_Chr6g0466051 30.204 490 323 8 87 565 66 547 9.73e-77 253
Msa0559010 MtrunA17_Chr2g0319041 29.901 505 340 9 71 565 51 551 9.74e-77 253
Msa0559010 MtrunA17_Chr5g0431651 29.842 506 336 7 71 565 49 546 8.44e-76 250
Msa0559010 MtrunA17_Chr5g0425371 30.339 501 330 8 78 565 53 547 6.37e-74 245
Msa0559010 MtrunA17_Chr7g0237011 29.707 478 315 7 89 555 13 480 3.81e-73 241
Msa0559010 MtrunA17_Chr7g0236961 29.707 478 315 7 89 555 13 480 6.58e-73 241
Msa0559010 MtrunA17_Chr7g0237001 29.762 504 328 9 67 555 43 535 7.08e-73 243
Msa0559010 MtrunA17_Chr7g0237041 29.762 504 328 9 67 555 43 535 7.08e-73 243
Msa0559010 MtrunA17_Chr7g0237081 29.762 504 328 9 67 555 43 535 7.08e-73 243
Msa0559010 MtrunA17_Chr7g0237051 29.762 504 328 9 67 555 43 535 7.08e-73 243
Msa0559010 MtrunA17_Chr7g0236991 29.762 504 328 9 67 555 43 535 7.86e-73 242
Msa0559010 MtrunA17_Chr8g0336181 30.943 488 317 10 71 547 51 529 1.71e-70 236
Msa0559010 MtrunA17_Chr2g0310401 34.709 412 258 6 149 553 5 412 7.95e-69 228
Msa0559010 MtrunA17_Chr4g0008621 31.218 394 256 8 49 433 37 424 1.63e-58 201
Msa0559010 MtrunA17_Chr6g0466111 35.374 294 177 4 87 373 31 318 3.07e-54 187
Msa0559010 MtrunA17_Chr6g0465931 35.374 294 177 4 87 373 31 318 3.23e-54 187
Msa0559010 MtrunA17_Chr6g0465981 35.374 294 177 4 87 373 66 353 5.06e-54 187
Msa0559010 MtrunA17_Chr6g0465871 35.374 294 177 4 87 373 66 353 5.06e-54 187
Msa0559010 MtrunA17_Chr6g0466081 35.374 294 177 4 87 373 66 353 5.06e-54 187
Msa0559010 MtrunA17_Chr7g0236971 28.093 388 267 5 175 555 1 383 1.01e-51 182
Msa0559010 MtrunA17_Chr7g0237031 33.219 292 182 4 89 373 13 298 1.92e-51 179
Msa0559010 MtrunA17_Chr7g0236951 33.219 292 182 4 89 373 13 298 2.46e-51 178
Msa0559010 MtrunA17_Chr7g0217361 35.862 290 167 7 272 551 8 288 1.37e-49 173
Msa0559010 MtrunA17_Chr8g0357231 36.328 256 155 6 315 565 1 253 1.38e-47 166
Msa0559010 MtrunA17_Chr2g0319071 26.598 485 301 12 89 565 46 483 6.03e-46 168
Msa0559010 MtrunA17_Chr2g0319081 28.428 299 189 7 275 565 140 421 3.46e-35 137
Msa0559010 MtrunA17_Chr5g0445511 31.064 235 157 3 171 402 2 234 1.46e-34 130
Msa0559010 MtrunA17_Chr2g0310121 26.172 512 331 14 89 565 276 775 7.34e-34 137
Msa0559010 MtrunA17_Chr6g0466031 27.451 255 179 4 315 565 2 254 1.12e-31 123
Msa0559010 MtrunA17_Chr3g0101681 26.747 415 264 11 89 473 277 681 2.54e-31 129
Msa0559010 MtrunA17_Chr7g0237061 28.099 242 149 3 315 555 2 219 1.42e-29 116
Msa0559010 MtrunA17_Chr7g0237071 28.571 196 127 4 89 277 13 202 6.04e-21 92.0
Msa0559010 MtrunA17_Chr6g0466021 36.170 141 80 3 87 223 66 200 4.72e-20 88.6
Msa0559010 MtrunA17_Chr4g0050281 61.194 67 24 1 7 71 37 103 2.00e-18 81.3
Msa0559010 MtrunA17_Chr8g0357221 27.679 224 151 5 55 274 74 290 3.13e-18 85.5
Msa0559010 MtrunA17_Chr2g0281651 22.659 331 242 8 231 553 24 348 4.01e-18 87.0
Msa0559010 MtrunA17_Chr6g0465881 24.551 167 122 2 402 565 1 166 4.89e-15 73.2
Msa0559010 MtrunA17_Chr6g0465941 24.551 167 122 2 402 565 1 166 5.59e-15 73.2
Msa0559010 MtrunA17_Chr6g0466121 24.551 167 122 2 402 565 1 166 5.59e-15 73.2
Msa0559010 MtrunA17_Chr6g0465991 24.551 167 122 2 402 565 1 166 5.59e-15 73.2
Msa0559010 MtrunA17_Chr6g0466091 24.551 167 122 2 402 565 1 166 5.59e-15 73.2
Msa0559010 MtrunA17_Chr5g0445501 25.150 167 121 2 402 565 1 166 3.06e-14 70.9
Msa0559010 MtrunA17_Chr7g0237021 26.582 158 108 3 402 555 1 154 1.11e-13 69.3
Msa0559010 MtrunA17_Chr7g0236941 26.582 158 108 3 402 555 1 154 1.11e-13 69.3
Msa0559010 MtrunA17_Chr7g0233861 30.282 142 84 4 209 347 29 158 1.05e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0559010 AT1G61680.1 41.828 569 304 9 8 565 17 569 1.59e-151 447
Msa0559010 AT1G61680.2 41.301 569 300 10 8 565 17 562 2.72e-147 436
Msa0559010 AT4G16730.1 31.445 512 325 10 76 565 26 533 3.25e-83 270
Msa0559010 AT4G16730.2 31.772 491 315 9 91 565 1 487 2.95e-82 266
Msa0559010 AT2G24210.1 31.818 528 333 13 56 565 66 584 2.64e-75 250
Msa0559010 AT4G16740.1 31.111 495 313 12 87 565 76 558 5.41e-73 243
Msa0559010 AT3G25810.1 31.275 502 321 12 83 565 95 591 8.74e-69 233
Msa0559010 AT3G25820.1 31.936 501 319 13 82 565 93 588 1.61e-68 233
Msa0559010 AT3G25830.1 31.936 501 319 13 82 565 93 588 1.61e-68 233
Msa0559010 AT5G23960.2 29.297 512 342 9 65 565 37 539 8.49e-67 226
Msa0559010 AT5G23960.1 29.183 514 342 10 65 565 37 541 2.18e-65 223
Msa0559010 AT1G70080.1 25.866 491 337 11 92 564 123 604 1.63e-47 175
Msa0559010 AT3G14490.1 25.738 474 338 8 83 549 107 573 1.27e-46 173
Msa0559010 AT3G14520.1 23.346 514 372 10 65 564 92 597 3.16e-46 172
Msa0559010 AT1G33750.1 24.152 501 360 7 78 565 104 597 1.55e-45 170
Msa0559010 AT4G16740.2 31.889 323 202 6 87 398 76 391 2.39e-45 165
Msa0559010 AT3G29410.2 25.102 490 348 8 87 565 11 492 2.86e-44 164
Msa0559010 AT3G14540.1 23.495 515 370 12 65 564 89 594 4.99e-44 166
Msa0559010 AT3G29410.1 24.805 512 359 9 72 565 94 597 7.26e-44 165
Msa0559010 AT3G25820.2 31.361 338 221 7 82 411 93 427 1.01e-43 162
Msa0559010 AT4G20200.1 23.735 514 370 9 68 565 91 598 1.51e-42 161
Msa0559010 AT5G44630.1 24.145 497 359 9 79 565 63 551 1.34e-41 158
Msa0559010 AT1G31950.3 23.494 498 347 12 87 564 117 600 1.37e-41 159
Msa0559010 AT1G31950.1 23.494 498 347 12 87 564 116 599 1.45e-41 159
Msa0559010 AT4G13300.1 24.897 486 348 9 91 565 69 548 4.14e-41 156
Msa0559010 AT2G23230.2 26.113 494 350 10 83 565 105 594 8.81e-41 156
Msa0559010 AT2G23230.1 26.465 495 342 11 83 565 105 589 1.03e-39 153
Msa0559010 AT3G29190.1 24.736 473 346 7 84 550 109 577 7.84e-39 150
Msa0559010 AT1G66020.1 25.662 491 352 9 84 565 106 592 1.77e-38 149
Msa0559010 AT4G13280.2 25.210 476 337 11 102 565 80 548 2.84e-38 148
Msa0559010 AT1G48800.1 26.104 498 342 14 83 564 112 599 9.76e-38 147
Msa0559010 AT5G48110.1 30.128 312 212 6 248 557 17 324 1.25e-37 142
Msa0559010 AT3G14540.2 22.892 415 309 7 65 472 89 499 1.57e-37 145
Msa0559010 AT4G15870.1 25.272 459 317 8 98 548 132 572 8.78e-37 145
Msa0559010 AT1G31950.2 23.373 415 294 9 87 487 117 521 1.02e-36 144
Msa0559010 AT4G20210.1 25.101 494 353 9 83 565 107 594 1.61e-36 144
Msa0559010 AT4G20230.1 24.750 501 345 10 84 565 112 599 1.82e-36 144
Msa0559010 AT4G20230.2 24.750 501 345 10 84 565 124 611 2.04e-36 144
Msa0559010 AT4G16740.4 31.250 272 170 5 87 347 76 341 9.33e-35 135
Msa0559010 AT4G16740.3 31.250 272 170 5 87 347 76 341 9.33e-35 135
Msa0559010 AT3G25820.4 32.117 274 175 7 82 347 93 363 1.31e-32 129
Msa0559010 AT3G25820.3 32.117 274 175 7 82 347 93 363 1.31e-32 129
Msa0559010 AT1G79460.1 25.536 513 332 14 89 565 275 773 1.83e-32 133
Msa0559010 AT3G32030.1 21.193 486 368 7 67 542 91 571 5.19e-32 130
Msa0559010 AT3G29110.1 25.403 496 331 10 91 564 112 590 2.54e-31 129
Msa0559010 AT4G13280.1 23.950 476 313 12 102 565 80 518 7.22e-29 120
Msa0559010 AT1G61120.1 23.636 495 336 12 89 553 286 768 2.38e-27 117
Msa0559010 AT1G48820.1 22.343 461 306 10 98 550 124 540 1.08e-26 114
Msa0559010 AT1G66020.2 24.739 287 207 6 84 364 106 389 4.20e-23 102

Find 126 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAATACTCCTCTGAATT+TGG 0.087434 4_1:+58034830 None:intergenic
AAACAAAAGCACAAATATTT+TGG 0.114515 4_1:-58037517 Msa0559010:CDS
ATCACATAGCCTTAGAATTT+TGG 0.127459 4_1:+58034682 None:intergenic
TATCATCAATGATGTATATT+AGG 0.172952 4_1:+58035463 None:intergenic
ATACTCCTTGTTTGGTTGTT+TGG 0.215702 4_1:-58033904 Msa0559010:three_prime_UTR
CAAGGTGTGTGCCACAAATT+TGG 0.217226 4_1:+58036289 None:intergenic
TACTCCTTGTTTGGTTGTTT+GGG 0.231065 4_1:-58033903 Msa0559010:three_prime_UTR
CCATCATAATTGTACATTAT+AGG 0.232144 4_1:+58034236 None:intergenic
TGAGGATATAAATGGATTAA+TGG 0.239175 4_1:-58036429 Msa0559010:CDS
ATTCAAGACAGTAGTTAAAT+TGG 0.250833 4_1:+58037449 None:intergenic
GAAGCAAAATGGTTTGCTTC+TGG 0.251074 4_1:-58034860 Msa0559010:CDS
GACCACGAGTACCATTTAAT+TGG 0.252942 4_1:+58035549 None:intergenic
TTGTTTGGGAGCTGGGAATT+AGG 0.275658 4_1:-58033889 Msa0559010:three_prime_UTR
CTTTCTTGCAAGAAGCAAAA+TGG 0.287959 4_1:-58034871 Msa0559010:CDS
GTCCTTGATATTCATTTCTC+TGG 0.295905 4_1:+58037094 None:intergenic
TTGTTTGGTTGTTTGGGAGC+TGG 0.298477 4_1:-58033897 Msa0559010:three_prime_UTR
CTAGAGATGAACCAATTAAA+TGG 0.303622 4_1:-58035560 Msa0559010:CDS
GAAACTAGAATAGGCATGTT+TGG 0.319225 4_1:+58037482 None:intergenic
TAACCAATGAATTTGCTCTT+AGG 0.320272 4_1:-58035237 Msa0559010:CDS
CTTAGGTTTGGCAAAGGACT+TGG 0.338530 4_1:-58035589 Msa0559010:CDS
TTTGGCTGTTGTAGATATAA+TGG 0.338929 4_1:+58034700 None:intergenic
GTATGTGAAATCGTTGCTTT+AGG 0.344788 4_1:-58034189 Msa0559010:exon
AAATCCAAAGCTTATTGTAT+TGG 0.348577 4_1:-58037643 Msa0559010:five_prime_UTR
CTACAACAGCCAAAATTCTA+AGG 0.352122 4_1:-58034691 Msa0559010:CDS
ATCATCAATGATGTATATTA+GGG 0.352665 4_1:+58035464 None:intergenic
TGAGAAAACCGTGTCTCTAA+TGG 0.359133 4_1:-58034735 Msa0559010:CDS
AAGCAAAATGGTTTGCTTCT+GGG 0.359768 4_1:-58034859 Msa0559010:CDS
TACAGACGAATGTTTATCTA+TGG 0.359859 4_1:-58037214 Msa0559010:CDS
GATAACCCAGGAGTTTCTAT+TGG 0.360210 4_1:-58034386 Msa0559010:CDS
AAATGGATATACTCCTTGTT+TGG 0.361315 4_1:-58033912 Msa0559010:intron
CATTCACACTTTATGTGACT+TGG 0.371148 4_1:+58033973 None:intergenic
TTTAACCTCATTAATAAAAT+CGG 0.373362 4_1:-58035188 Msa0559010:intron
TGTTTGGTTGTTTGGGAGCT+GGG 0.377861 4_1:-58033896 Msa0559010:three_prime_UTR
ATGACCAGGCATTAAGATAT+TGG 0.379692 4_1:+58036336 None:intergenic
GGCACATTACTAATAATCTA+TGG 0.381390 4_1:-58037589 Msa0559010:exon
GCTTCTCAATTGAGCATAGA+AGG 0.389737 4_1:-58036395 Msa0559010:CDS
TGAAGAAAATGTTGAATGAT+TGG 0.395622 4_1:+58037539 None:intergenic
GAAACCAATACAATAAGCTT+TGG 0.402507 4_1:+58037639 None:intergenic
CTGCAGAATGCTTTGATGTT+GGG 0.412622 4_1:+58034133 None:intergenic
GTTTGTCCAATAGAAACTCC+TGG 0.424695 4_1:+58034380 None:intergenic
TGTGAGCTCAATCCTATCTT+CGG 0.431112 4_1:+58035497 None:intergenic
CACTTTATGTGACTTGGGTT+CGG 0.432033 4_1:+58033979 None:intergenic
GTTAATTGTGAGGGTAATGA+TGG 0.436983 4_1:-58034434 Msa0559010:CDS
AAACTAGAATAGGCATGTTT+GGG 0.438056 4_1:+58037483 None:intergenic
GTTTGGACGCAGATGGGACT+TGG 0.438150 4_1:-58035317 Msa0559010:intron
GTGTACCCCTGTGCTTACTA+TGG 0.438421 4_1:+58034802 None:intergenic
TTAGTAAAAGATGATGGTAA+TGG 0.444587 4_1:+58034287 None:intergenic
GTGCCACAAATTTGGCTTGA+GGG 0.445407 4_1:+58036297 None:intergenic
TTTATTATAGAAACTAGAAT+AGG 0.448429 4_1:+58037473 None:intergenic
CAAATTCATACTCCTAAAAG+TGG 0.456424 4_1:-58037426 Msa0559010:CDS
GCCTGGTCATCAACATTTCA+TGG 0.462096 4_1:-58036323 Msa0559010:CDS
TCTTCGGAGAACTGTGGACC+TGG 0.467503 4_1:+58035513 None:intergenic
GTCAACAAATTTCAGATGCA+TGG 0.469176 4_1:-58034343 Msa0559010:CDS
GTCCACAGTTCTCCGAAGAT+AGG 0.469625 4_1:-58035509 Msa0559010:CDS
CTGCATGAAAGTGTGTTTCA+AGG 0.472196 4_1:-58035272 Msa0559010:CDS
TATAGATGGTGGAAAGACTT+AGG 0.478515 4_1:-58035606 Msa0559010:intron
TTCAGAAATAGACACTCAAA+TGG 0.482805 4_1:-58036177 Msa0559010:CDS
GACTTGGGTTCGGTAAAATC+AGG 0.492596 4_1:+58033989 None:intergenic
AAACACACTTTCATGCAGTC+TGG 0.496127 4_1:+58035277 None:intergenic
ATGGTGGAAAGACTTAGGTT+TGG 0.496137 4_1:-58035601 Msa0559010:CDS
TGGTCAATGGCATGTCTCCC+AGG 0.498526 4_1:-58035531 Msa0559010:CDS
TCTAAGGCTATGTGATGACT+TGG 0.498674 4_1:-58034675 Msa0559010:CDS
TGTCCTAAGAGCAAATTCAT+TGG 0.505116 4_1:+58035234 None:intergenic
AGCATTGGATGAACTTAAGC+AGG 0.506378 4_1:-58037262 Msa0559010:CDS
TTTATGAATTGGATACATCA+AGG 0.506618 4_1:+58036271 None:intergenic
TATAAGACATGCAAAAGCAT+TGG 0.509430 4_1:-58037277 Msa0559010:CDS
TTTCGTATGTTGAGACAAGA+AGG 0.509440 4_1:-58037051 Msa0559010:CDS
CCTATAATGTACAATTATGA+TGG 0.511514 4_1:-58034236 Msa0559010:CDS
AGAATAGCATAGACACACTT+TGG 0.511989 4_1:-58033865 Msa0559010:three_prime_UTR
ATTCACACTTTATGTGACTT+GGG 0.518172 4_1:+58033974 None:intergenic
TTCCAGAGAAATGAATATCA+AGG 0.522241 4_1:-58037096 Msa0559010:CDS
ACTGCAGAATGCTTTGATGT+TGG 0.532928 4_1:+58034132 None:intergenic
GTGGATTCCATCAAAAGATT+AGG 0.535767 4_1:-58037192 Msa0559010:CDS
TTTGTCCAATAGAAACTCCT+GGG 0.537264 4_1:+58034381 None:intergenic
AGACGAATGTTTATCTATGG+TGG 0.538797 4_1:-58037211 Msa0559010:CDS
GCCACCAATATCTTAATGCC+TGG 0.539711 4_1:-58036340 Msa0559010:CDS
GCTGTTGTAGATATAATGGT+TGG 0.540737 4_1:+58034704 None:intergenic
TAAATGGTACTCGTGGTCAA+TGG 0.541293 4_1:-58035544 Msa0559010:CDS
CAAAGTTTAGTAAAAGATGA+TGG 0.541713 4_1:+58034281 None:intergenic
AAGAATGCCATAGTAAGCAC+AGG 0.541801 4_1:-58034809 Msa0559010:CDS
AGATTATTAGTAATGTGCCT+TGG 0.543952 4_1:+58037593 None:intergenic
CCACCCTCAAGCCAAATTTG+TGG 0.551722 4_1:-58036300 Msa0559010:CDS
TTCCATCAAAAGATTAGGCA+TGG 0.555371 4_1:-58037187 Msa0559010:CDS
GCATAGACACACTTTGGAAG+AGG 0.555805 4_1:-58033859 Msa0559010:three_prime_UTR
TGCAGAATGCTTTGATGTTG+GGG 0.557340 4_1:+58034134 None:intergenic
CTGCAGGATGTTAATTGTGA+GGG 0.561151 4_1:-58034443 Msa0559010:intron
GGTTTCATCATCACATCTCA+AGG 0.564772 4_1:-58036156 Msa0559010:CDS
GAAGGGTATTACATTAGCCC+AGG 0.567931 4_1:-58037033 Msa0559010:intron
TTGTCTCAATGCTGCACGGA+TGG 0.569745 4_1:-58034261 Msa0559010:CDS
TCCTAAAAGTGGAAAATGCA+AGG 0.571235 4_1:-58037415 Msa0559010:intron
CTTAGGACATACATCAAACA+TGG 0.577045 4_1:-58035220 Msa0559010:CDS
CTGTTGTAGATATAATGGTT+GGG 0.577593 4_1:+58034705 None:intergenic
GAAGACTGTCTTGACAATGT+AGG 0.580932 4_1:-58036371 Msa0559010:CDS
GTGAAATCGTTGCTTTAGGA+TGG 0.581438 4_1:-58034185 Msa0559010:exon
AAATCGTTGCTTTAGGATGG+TGG 0.583144 4_1:-58034182 Msa0559010:exon
AACTTTGTCTCAATGCTGCA+CGG 0.584173 4_1:-58034265 Msa0559010:CDS
GGACATACATCAAACATGGA+TGG 0.590806 4_1:-58035216 Msa0559010:CDS
GCCATGAAATGTTGATGACC+AGG 0.591213 4_1:+58036322 None:intergenic
TGTGCCACAAATTTGGCTTG+AGG 0.592343 4_1:+58036296 None:intergenic
AGCTCCCAAACAACCAAACA+AGG 0.596345 4_1:+58033899 None:intergenic
AAATTGAAGCAACACTTGAA+AGG 0.596654 4_1:-58037146 Msa0559010:CDS
ACCAGGCATTAAGATATTGG+TGG 0.597715 4_1:+58036339 None:intergenic
CAATACTTCTTCAAAGCTAG+AGG 0.600961 4_1:-58034213 Msa0559010:CDS
CATCTCTCTTACTCAAACCA+AGG 0.603238 4_1:-58037610 Msa0559010:five_prime_UTR
ACTGCAGGATGTTAATTGTG+AGG 0.605083 4_1:-58034444 Msa0559010:intron
TGCAATCACAAAATGCAGCA+TGG 0.607198 4_1:-58036220 Msa0559010:CDS
AGTTTCTATTGGACAAACAA+AGG 0.608984 4_1:-58034375 Msa0559010:CDS
TGGGAACGTTCCAAATTCAG+AGG 0.609438 4_1:-58034840 Msa0559010:CDS
GAAAGACTTAGGTTTGGCAA+AGG 0.612512 4_1:-58035595 Msa0559010:CDS
GAAACTCTTAAATCTAAGCA+TGG 0.613254 4_1:+58037116 None:intergenic
CTTCGGAGAACTGTGGACCT+GGG 0.616156 4_1:+58035514 None:intergenic
TGATGACTTGGAAGGCGACA+AGG 0.619282 4_1:-58034663 Msa0559010:intron
TCTTTACAGATGCTGTTGAG+AGG 0.626983 4_1:-58035404 Msa0559010:intron
AGGCTATGTGATGACTTGGA+AGG 0.633610 4_1:-58034671 Msa0559010:CDS
AACCAATTAAATGGTACTCG+TGG 0.634167 4_1:-58035551 Msa0559010:CDS
TTCGTATGTTGAGACAAGAA+GGG 0.638397 4_1:-58037050 Msa0559010:CDS
ATGATGTATATTAGGGACAG+AGG 0.640251 4_1:+58035471 None:intergenic
GAAGTCATCCATTAGAGACA+CGG 0.643126 4_1:+58034727 None:intergenic
ATCCTATCTTCGGAGAACTG+TGG 0.643675 4_1:+58035507 None:intergenic
CCACAAATTTGGCTTGAGGG+TGG 0.654843 4_1:+58036300 None:intergenic
CAATTGAGAAGCTTCAAACA+AGG 0.656364 4_1:+58036406 None:intergenic
TGCATGGAAACAACTCAACA+AGG 0.663635 4_1:-58034327 Msa0559010:CDS
AGAATGCCATAGTAAGCACA+GGG 0.674782 4_1:-58034808 Msa0559010:CDS
TTAATTGTGAGGGTAATGAT+GGG 0.700457 4_1:-58034433 Msa0559010:CDS
TGTTACATGAAAGATAACCC+AGG 0.716647 4_1:-58034398 Msa0559010:CDS
GAATGCCATAGTAAGCACAG+GGG 0.839728 4_1:-58034807 Msa0559010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAAATTGTTTGTTT+AGG - chr4_1:58034466-58034485 Msa0559010:intron 10.0%
!!! AAAAAATAGAAACTTTTTTT+GGG + chr4_1:58035484-58035503 None:intergenic 10.0%
!!! TAAAAAATAGAAACTTTTTT+TGG + chr4_1:58035485-58035504 None:intergenic 10.0%
!!! TGATTTTTTCAAATTATTTA+CGG + chr4_1:58036325-58036344 None:intergenic 10.0%
!! TTTATTATAGAAACTAGAAT+AGG + chr4_1:58033919-58033938 None:intergenic 15.0%
!! TCAAACTTCAAATAAATTAT+AGG - chr4_1:58034541-58034560 Msa0559010:intron 15.0%
!! CAAACTTCAAATAAATTATA+GGG - chr4_1:58034542-58034561 Msa0559010:intron 15.0%
!!! AATTTTTATGAAAGTTAAGT+AGG + chr4_1:58035286-58035305 None:intergenic 15.0%
!!! TTTTTTCAAGAAAAAACAAA+TGG - chr4_1:58035406-58035425 Msa0559010:CDS 15.0%
!!! ATTTTTTTTTAATGATTTGG+AGG + chr4_1:58035610-58035629 None:intergenic 15.0%
!!! GTTTAATTTTTATACATTGT+CGG + chr4_1:58035704-58035723 None:intergenic 15.0%
!!! GATGATATATTTGATTTTTA+TGG - chr4_1:58035942-58035961 Msa0559010:intron 15.0%
!!! TTTAATTTTTTATTTGAGTG+AGG + chr4_1:58036016-58036035 None:intergenic 15.0%
!!! TAAGATTTTTTTTACTAGAA+TGG + chr4_1:58036039-58036058 None:intergenic 15.0%
!! TTTAACCTCATTAATAAAAT+CGG - chr4_1:58036201-58036220 Msa0559010:CDS 15.0%
!! TTAATGAATTGTGATTAATT+TGG - chr4_1:58036459-58036478 Msa0559010:CDS 15.0%
!! TAATGAATTGTGATTAATTT+GGG - chr4_1:58036460-58036479 Msa0559010:CDS 15.0%
!!! TTAATTCCACTTTAAAATAT+TGG - chr4_1:58036780-58036799 Msa0559010:intron 15.0%
!!! TTATTTTAAATAATTACTGC+AGG - chr4_1:58036930-58036949 Msa0559010:intron 15.0%
!!! GTTGATATTTTAATAAAATG+AGG - chr4_1:58037593-58037612 Msa0559010:five_prime_UTR 15.0%
!!! AAACAAAAGCACAAATATTT+TGG - chr4_1:58033872-58033891 Msa0559010:three_prime_UTR 20.0%
!!! TCTTTTTCTTTAGTTTCATA+TGG - chr4_1:58034018-58034037 Msa0559010:three_prime_UTR 20.0%
!! TCCTAAATGATTAATTGAAA+GGG + chr4_1:58034083-58034102 None:intergenic 20.0%
!! ATCCTAAATGATTAATTGAA+AGG + chr4_1:58034084-58034103 None:intergenic 20.0%
!! CTTAGATAAGTATATGTAAT+TGG + chr4_1:58034832-58034851 None:intergenic 20.0%
!!! TTTTTTATCAAAATGACACA+TGG - chr4_1:58035514-58035533 Msa0559010:CDS 20.0%
!!! ATATGTATGACTTTAAAAGT+AGG + chr4_1:58035647-58035666 None:intergenic 20.0%
!! TGATGTAATCATTAGTAATA+CGG + chr4_1:58035748-58035767 None:intergenic 20.0%
!! GATTACATCATTTATAATCA+TGG - chr4_1:58035758-58035777 Msa0559010:intron 20.0%
!! TTATAATCATGGTATATAGA+TGG - chr4_1:58035769-58035788 Msa0559010:intron 20.0%
!! ATCATCAATGATGTATATTA+GGG + chr4_1:58035928-58035947 None:intergenic 20.0%
!! TATCATCAATGATGTATATT+AGG + chr4_1:58035929-58035948 None:intergenic 20.0%
!!! GATTTTATTAATGAGGTTAA+AGG + chr4_1:58036202-58036221 None:intergenic 20.0%
!!! TACTAAAGATTTAGAATGAA+GGG + chr4_1:58036231-58036250 None:intergenic 20.0%
!! ATTAATGAAGTTAGAAACAT+AGG + chr4_1:58036765-58036784 None:intergenic 20.0%
!! ATGTACCAAAGAATAAATAT+TGG - chr4_1:58036835-58036854 Msa0559010:intron 20.0%
!! CTAATAATTTAGTATGATTG+TGG + chr4_1:58036862-58036881 None:intergenic 20.0%
!! CAATCATACTAAATTATTAG+TGG - chr4_1:58036862-58036881 Msa0559010:intron 20.0%
!! ACAAACAAATAAAACTAACA+TGG - chr4_1:58036893-58036912 Msa0559010:intron 20.0%
! AAATCCAAAGCTTATTGTAT+TGG - chr4_1:58033746-58033765 Msa0559010:three_prime_UTR 25.0%
! TGAAGAAAATGTTGAATGAT+TGG + chr4_1:58033853-58033872 None:intergenic 25.0%
! ATTCAAGACAGTAGTTAAAT+TGG + chr4_1:58033943-58033962 None:intergenic 25.0%
! CTTAATTAGCTACAAGTTAA+TGG + chr4_1:58034056-58034075 None:intergenic 25.0%
! ACCCTTTCAATTAATCATTT+AGG - chr4_1:58034079-58034098 Msa0559010:three_prime_UTR 25.0%
!!! ATTAGATTGGTTTTCAACTT+TGG - chr4_1:58034425-58034444 Msa0559010:CDS 25.0%
! ATAGAAAATTTGTGAGGAAA+AGG + chr4_1:58034634-58034653 None:intergenic 25.0%
! TTGAGTATAGAAAATTTGTG+AGG + chr4_1:58034640-58034659 None:intergenic 25.0%
!! TTTAGGATATCAACAATGTT+AGG + chr4_1:58034724-58034743 None:intergenic 25.0%
!!! ACATTTTCTGAGGATATAAA+TGG - chr4_1:58034952-58034971 Msa0559010:intron 25.0%
!!! TGAGGATATAAATGGATTAA+TGG - chr4_1:58034960-58034979 Msa0559010:intron 25.0%
! TTTATGAATTGGATACATCA+AGG + chr4_1:58035121-58035140 None:intergenic 25.0%
!!! CTTGACAAAGTTTTATGAAT+TGG + chr4_1:58035132-58035151 None:intergenic 25.0%
!! TTTTGTGATTGCATTATTGT+TGG + chr4_1:58035162-58035181 None:intergenic 25.0%
!!! GTACGTTTTTAAAAGATTCA+AGG - chr4_1:58035307-58035326 Msa0559010:CDS 25.0%
! GTATTTGATTCATTAACATG+TGG + chr4_1:58035333-58035352 None:intergenic 25.0%
! TTTCAAGAAAAAACAAATGG+CGG - chr4_1:58035409-58035428 Msa0559010:CDS 25.0%
!! CTTAATAGCATTCTTTTGTT+TGG - chr4_1:58036055-58036074 Msa0559010:intron 25.0%
!!! GAATACCGATTTTATTAATG+AGG + chr4_1:58036209-58036228 None:intergenic 25.0%
!! CTACTAAAGATTTAGAATGA+AGG + chr4_1:58036232-58036251 None:intergenic 25.0%
! TCAAGTGTAATTATATGTGT+CGG - chr4_1:58036350-58036369 Msa0559010:CDS 25.0%
!! TATGAAGCACAAATACTTTT+CGG + chr4_1:58036417-58036436 None:intergenic 25.0%
!!! TTTTTCTAGGGGATATTTTT+TGG + chr4_1:58036816-58036835 None:intergenic 25.0%
!! TTCTTTGGTACATTTTTCTA+GGG + chr4_1:58036828-58036847 None:intergenic 25.0%
!! ATTCTTTGGTACATTTTTCT+AGG + chr4_1:58036829-58036848 None:intergenic 25.0%
!! TTAGTAAAAGATGATGGTAA+TGG + chr4_1:58037105-58037124 None:intergenic 25.0%
! CAAAGTTTAGTAAAAGATGA+TGG + chr4_1:58037111-58037130 None:intergenic 25.0%
! CCATCATAATTGTACATTAT+AGG + chr4_1:58037156-58037175 None:intergenic 25.0%
! CCTATAATGTACAATTATGA+TGG - chr4_1:58037153-58037172 Msa0559010:CDS 25.0%
GAAACCAATACAATAAGCTT+TGG + chr4_1:58033753-58033772 None:intergenic 30.0%
AGATTATTAGTAATGTGCCT+TGG + chr4_1:58033799-58033818 None:intergenic 30.0%
GGCACATTACTAATAATCTA+TGG - chr4_1:58033800-58033819 Msa0559010:three_prime_UTR 30.0%
AAACTAGAATAGGCATGTTT+GGG + chr4_1:58033909-58033928 None:intergenic 30.0%
CAAATTCATACTCCTAAAAG+TGG - chr4_1:58033963-58033982 Msa0559010:intron 30.0%
! TATAAGACATGCAAAAGCAT+TGG - chr4_1:58034112-58034131 Msa0559010:three_prime_UTR 30.0%
TACAGACGAATGTTTATCTA+TGG - chr4_1:58034175-58034194 Msa0559010:exon 30.0%
AAATTGAAGCAACACTTGAA+AGG - chr4_1:58034243-58034262 Msa0559010:CDS 30.0%
GAAACTCTTAAATCTAAGCA+TGG + chr4_1:58034276-58034295 None:intergenic 30.0%
TTCCAGAGAAATGAATATCA+AGG - chr4_1:58034293-58034312 Msa0559010:CDS 30.0%
CCAATCTAATCAATGTAGAT+TGG + chr4_1:58034415-58034434 None:intergenic 30.0%
! CCAATCTACATTGATTAGAT+TGG - chr4_1:58034412-58034431 Msa0559010:CDS 30.0%
TAAGATTCAGCGAAAAAACA+AGG + chr4_1:58034885-58034904 None:intergenic 30.0%
!! GATAAGTTTTGTGACAACAA+AGG - chr4_1:58034916-58034935 Msa0559010:intron 30.0%
TTCAGAAATAGACACTCAAA+TGG - chr4_1:58035212-58035231 Msa0559010:CDS 30.0%
TAATCATGGTATATAGATGG+TGG - chr4_1:58035772-58035791 Msa0559010:intron 30.0%
CTAGAGATGAACCAATTAAA+TGG - chr4_1:58035829-58035848 Msa0559010:intron 30.0%
! TAACCAATGAATTTGCTCTT+AGG - chr4_1:58036152-58036171 Msa0559010:CDS 30.0%
TTTCATAGCATGATACACAT+AGG + chr4_1:58036262-58036281 None:intergenic 30.0%
TTCAAATACTCCTCTGAATT+TGG + chr4_1:58036562-58036581 None:intergenic 30.0%
CTGTTGTAGATATAATGGTT+GGG + chr4_1:58036687-58036706 None:intergenic 30.0%
TTTGGCTGTTGTAGATATAA+TGG + chr4_1:58036692-58036711 None:intergenic 30.0%
!! ATCACATAGCCTTAGAATTT+TGG + chr4_1:58036710-58036729 None:intergenic 30.0%
!! TGTGGTCCAATATTTTAAAG+TGG + chr4_1:58036789-58036808 None:intergenic 30.0%
! TCTTTGGTACATTTTTCTAG+GGG + chr4_1:58036827-58036846 None:intergenic 30.0%
GTGGTCCAATATTTATTCTT+TGG + chr4_1:58036843-58036862 None:intergenic 30.0%
TTAATTGTGAGGGTAATGAT+GGG - chr4_1:58036956-58036975 Msa0559010:intron 30.0%
AGTTTCTATTGGACAAACAA+AGG - chr4_1:58037014-58037033 Msa0559010:intron 30.0%
ATTCACACTTTATGTGACTT+GGG + chr4_1:58037418-58037437 None:intergenic 30.0%
AAATGGATATACTCCTTGTT+TGG - chr4_1:58037477-58037496 Msa0559010:CDS 30.0%
GAAACTAGAATAGGCATGTT+TGG + chr4_1:58033910-58033929 None:intergenic 35.0%
TCCTAAAAGTGGAAAATGCA+AGG - chr4_1:58033974-58033993 Msa0559010:intron 35.0%
!! ACCTTGCATTTTCCACTTTT+AGG + chr4_1:58033978-58033997 None:intergenic 35.0%
AGACGAATGTTTATCTATGG+TGG - chr4_1:58034178-58034197 Msa0559010:exon 35.0%
GTGGATTCCATCAAAAGATT+AGG - chr4_1:58034197-58034216 Msa0559010:CDS 35.0%
TTCCATCAAAAGATTAGGCA+TGG - chr4_1:58034202-58034221 Msa0559010:CDS 35.0%
!! CATGGAGTACAATTTTGAAG+AGG - chr4_1:58034220-58034239 Msa0559010:CDS 35.0%
GTCCTTGATATTCATTTCTC+TGG + chr4_1:58034298-58034317 None:intergenic 35.0%
TTTCGTATGTTGAGACAAGA+AGG - chr4_1:58034338-58034357 Msa0559010:CDS 35.0%
TTCGTATGTTGAGACAAGAA+GGG - chr4_1:58034339-58034358 Msa0559010:CDS 35.0%
ACTTGAAAGCAAACTAACCT+GGG + chr4_1:58034376-58034395 None:intergenic 35.0%
TACTTGAAAGCAAACTAACC+TGG + chr4_1:58034377-58034396 None:intergenic 35.0%
!! AGGTTAGTTTGCTTTCAAGT+AGG - chr4_1:58034376-58034395 Msa0559010:CDS 35.0%
TTGTCTAATAGAAACCCATG+TGG - chr4_1:58034668-58034687 Msa0559010:CDS 35.0%
!! AGTGCTGTGTGACTTATTTT+AGG - chr4_1:58034693-58034712 Msa0559010:CDS 35.0%
AAAAACAAGGTCATGGTCAT+GGG + chr4_1:58034872-58034891 None:intergenic 35.0%
AAAAAACAAGGTCATGGTCA+TGG + chr4_1:58034873-58034892 None:intergenic 35.0%
! ACTCAAGCATACATTTTCTG+AGG - chr4_1:58034942-58034961 Msa0559010:intron 35.0%
CAATTGAGAAGCTTCAAACA+AGG + chr4_1:58034986-58035005 None:intergenic 35.0%
ATGACCAGGCATTAAGATAT+TGG + chr4_1:58035056-58035075 None:intergenic 35.0%
AGTTAAGTAGGACTCTTACT+TGG + chr4_1:58035274-58035293 None:intergenic 35.0%
!! TATAGATGGTGGAAAGACTT+AGG - chr4_1:58035783-58035802 Msa0559010:intron 35.0%
AACCAATTAAATGGTACTCG+TGG - chr4_1:58035838-58035857 Msa0559010:intron 35.0%
ATGATGTATATTAGGGACAG+AGG + chr4_1:58035921-58035940 None:intergenic 35.0%
TGTCCTAAGAGCAAATTCAT+TGG + chr4_1:58036158-58036177 None:intergenic 35.0%
CTTAGGACATACATCAAACA+TGG - chr4_1:58036169-58036188 Msa0559010:CDS 35.0%
GTGTATCATGCTATGAAACA+AGG - chr4_1:58036264-58036283 Msa0559010:CDS 35.0%
ATGCTATGAAACAAGGACAT+CGG - chr4_1:58036271-58036290 Msa0559010:CDS 35.0%
! GCACAAATACTTTTCGGATT+AGG + chr4_1:58036411-58036430 None:intergenic 35.0%
TGGGTGTTAATTAATTGCAG+TGG - chr4_1:58036479-58036498 Msa0559010:CDS 35.0%
GGGTGTTAATTAATTGCAGT+GGG - chr4_1:58036480-58036499 Msa0559010:CDS 35.0%
CTTTCTTGCAAGAAGCAAAA+TGG - chr4_1:58036518-58036537 Msa0559010:intron 35.0%
!! AAGCAAAATGGTTTGCTTCT+GGG - chr4_1:58036530-58036549 Msa0559010:intron 35.0%
GCTGTTGTAGATATAATGGT+TGG + chr4_1:58036688-58036707 None:intergenic 35.0%
CTACAACAGCCAAAATTCTA+AGG - chr4_1:58036698-58036717 Msa0559010:intron 35.0%
!!! GGGATATTTTTTGGTACATG+TGG + chr4_1:58036807-58036826 None:intergenic 35.0%
GTTAATTGTGAGGGTAATGA+TGG - chr4_1:58036955-58036974 Msa0559010:intron 35.0%
TGTTACATGAAAGATAACCC+AGG - chr4_1:58036991-58037010 Msa0559010:intron 35.0%
TTTGTCCAATAGAAACTCCT+GGG + chr4_1:58037011-58037030 None:intergenic 35.0%
GTCAACAAATTTCAGATGCA+TGG - chr4_1:58037046-58037065 Msa0559010:CDS 35.0%
CAATACTTCTTCAAAGCTAG+AGG - chr4_1:58037176-58037195 Msa0559010:CDS 35.0%
! GTATGTGAAATCGTTGCTTT+AGG - chr4_1:58037200-58037219 Msa0559010:CDS 35.0%
CATTCACACTTTATGTGACT+TGG + chr4_1:58037419-58037438 None:intergenic 35.0%
! ATACTCCTTGTTTGGTTGTT+TGG - chr4_1:58037485-58037504 Msa0559010:CDS 35.0%
! TACTCCTTGTTTGGTTGTTT+GGG - chr4_1:58037486-58037505 Msa0559010:CDS 35.0%
AGAATAGCATAGACACACTT+TGG - chr4_1:58037524-58037543 Msa0559010:CDS 35.0%
CATCTCTCTTACTCAAACCA+AGG - chr4_1:58033779-58033798 Msa0559010:three_prime_UTR 40.0%
! AGCATTGGATGAACTTAAGC+AGG - chr4_1:58034127-58034146 Msa0559010:three_prime_UTR 40.0%
! CTCCATGCCTAATCTTTTGA+TGG + chr4_1:58034207-58034226 None:intergenic 40.0%
AAAACAAGGTCATGGTCATG+GGG + chr4_1:58034871-58034890 None:intergenic 40.0%
TCAGCGAAAAAACAAGGTCA+TGG + chr4_1:58034879-58034898 None:intergenic 40.0%
GCTTCTCAATTGAGCATAGA+AGG - chr4_1:58034994-58035013 Msa0559010:intron 40.0%
GAAGACTGTCTTGACAATGT+AGG - chr4_1:58035018-58035037 Msa0559010:intron 40.0%
ACCAGGCATTAAGATATTGG+TGG + chr4_1:58035053-58035072 None:intergenic 40.0%
TGCAATCACAAAATGCAGCA+TGG - chr4_1:58035169-58035188 Msa0559010:intron 40.0%
GGTTTCATCATCACATCTCA+AGG - chr4_1:58035233-58035252 Msa0559010:CDS 40.0%
! ATGGTGGAAAGACTTAGGTT+TGG - chr4_1:58035788-58035807 Msa0559010:intron 40.0%
GAAAGACTTAGGTTTGGCAA+AGG - chr4_1:58035794-58035813 Msa0559010:intron 40.0%
GACCACGAGTACCATTTAAT+TGG + chr4_1:58035843-58035862 None:intergenic 40.0%
TAAATGGTACTCGTGGTCAA+TGG - chr4_1:58035845-58035864 Msa0559010:intron 40.0%
TGTGAGCTCAATCCTATCTT+CGG + chr4_1:58035895-58035914 None:intergenic 40.0%
TCTTTACAGATGCTGTTGAG+AGG - chr4_1:58035985-58036004 Msa0559010:intron 40.0%
!! TTCTTTTGTTTGGACGCAGA+TGG - chr4_1:58036065-58036084 Msa0559010:intron 40.0%
!! TCTTTTGTTTGGACGCAGAT+GGG - chr4_1:58036066-58036085 Msa0559010:intron 40.0%
AAACACACTTTCATGCAGTC+TGG + chr4_1:58036115-58036134 None:intergenic 40.0%
! CTGCATGAAAGTGTGTTTCA+AGG - chr4_1:58036117-58036136 Msa0559010:intron 40.0%
GGACATACATCAAACATGGA+TGG - chr4_1:58036173-58036192 Msa0559010:CDS 40.0%
! GTAATTATATGTGTCGGCGT+CGG - chr4_1:58036356-58036375 Msa0559010:CDS 40.0%
!! TTTTCGGATTAGGCATGTCT+GGG + chr4_1:58036401-58036420 None:intergenic 40.0%
!! GAAGCAAAATGGTTTGCTTC+TGG - chr4_1:58036529-58036548 Msa0559010:intron 40.0%
AAGAATGCCATAGTAAGCAC+AGG - chr4_1:58036580-58036599 Msa0559010:intron 40.0%
AGAATGCCATAGTAAGCACA+GGG - chr4_1:58036581-58036600 Msa0559010:intron 40.0%
! TGTGCATGCTTTCTTTTGCA+TGG - chr4_1:58036618-58036637 Msa0559010:intron 40.0%
! GTGCATGCTTTCTTTTGCAT+GGG - chr4_1:58036619-58036638 Msa0559010:intron 40.0%
TGAGAAAACCGTGTCTCTAA+TGG - chr4_1:58036654-58036673 Msa0559010:intron 40.0%
GAAGTCATCCATTAGAGACA+CGG + chr4_1:58036665-58036684 None:intergenic 40.0%
! TCTAAGGCTATGTGATGACT+TGG - chr4_1:58036714-58036733 Msa0559010:intron 40.0%
ACTGCAGGATGTTAATTGTG+AGG - chr4_1:58036945-58036964 Msa0559010:intron 40.0%
CTGCAGGATGTTAATTGTGA+GGG - chr4_1:58036946-58036965 Msa0559010:intron 40.0%
GATAACCCAGGAGTTTCTAT+TGG - chr4_1:58037003-58037022 Msa0559010:intron 40.0%
GTTTGTCCAATAGAAACTCC+TGG + chr4_1:58037012-58037031 None:intergenic 40.0%
TGCATGGAAACAACTCAACA+AGG - chr4_1:58037062-58037081 Msa0559010:CDS 40.0%
AACTTTGTCTCAATGCTGCA+CGG - chr4_1:58037124-58037143 Msa0559010:CDS 40.0%
!! GTGAAATCGTTGCTTTAGGA+TGG - chr4_1:58037204-58037223 Msa0559010:CDS 40.0%
!! AAATCGTTGCTTTAGGATGG+TGG - chr4_1:58037207-58037226 Msa0559010:CDS 40.0%
TGCAGAATGCTTTGATGTTG+GGG + chr4_1:58037258-58037277 None:intergenic 40.0%
CTGCAGAATGCTTTGATGTT+GGG + chr4_1:58037259-58037278 None:intergenic 40.0%
ACTGCAGAATGCTTTGATGT+TGG + chr4_1:58037260-58037279 None:intergenic 40.0%
! CACTTTATGTGACTTGGGTT+CGG + chr4_1:58037413-58037432 None:intergenic 40.0%
AATGTCCAGTCATATGTGCT+TGG - chr4_1:58037435-58037454 Msa0559010:CDS 40.0%
ATGTCCAGTCATATGTGCTT+GGG - chr4_1:58037436-58037455 Msa0559010:CDS 40.0%
!! GAAGGGTATTACATTAGCCC+AGG - chr4_1:58034356-58034375 Msa0559010:CDS 45.0%
GCCACCAATATCTTAATGCC+TGG - chr4_1:58035049-58035068 Msa0559010:intron 45.0%
GCCTGGTCATCAACATTTCA+TGG - chr4_1:58035066-58035085 Msa0559010:intron 45.0%
!! GCCATGAAATGTTGATGACC+AGG + chr4_1:58035070-58035089 None:intergenic 45.0%
!! GTGCCACAAATTTGGCTTGA+GGG + chr4_1:58035095-58035114 None:intergenic 45.0%
!! TGTGCCACAAATTTGGCTTG+AGG + chr4_1:58035096-58035115 None:intergenic 45.0%
CAAGGTGTGTGCCACAAATT+TGG + chr4_1:58035103-58035122 None:intergenic 45.0%
CTTAGGTTTGGCAAAGGACT+TGG - chr4_1:58035800-58035819 Msa0559010:intron 45.0%
ATCCTATCTTCGGAGAACTG+TGG + chr4_1:58035885-58035904 None:intergenic 45.0%
!! CTTTTCGGATTAGGCATGTC+TGG + chr4_1:58036402-58036421 None:intergenic 45.0%
TGGGAACGTTCCAAATTCAG+AGG - chr4_1:58036549-58036568 Msa0559010:intron 45.0%
GAATGCCATAGTAAGCACAG+GGG - chr4_1:58036582-58036601 Msa0559010:intron 45.0%
! GCTTTCTTTTGCATGGGTCA+AGG - chr4_1:58036625-58036644 Msa0559010:intron 45.0%
! AGGCTATGTGATGACTTGGA+AGG - chr4_1:58036718-58036737 Msa0559010:intron 45.0%
! GACTTGGGTTCGGTAAAATC+AGG + chr4_1:58037403-58037422 None:intergenic 45.0%
AAGTCCCAAGCACATATGAC+TGG + chr4_1:58037443-58037462 None:intergenic 45.0%
CTTGCGAGTTAGGAGCAAAA+TGG - chr4_1:58037460-58037479 Msa0559010:CDS 45.0%
AGCTCCCAAACAACCAAACA+AGG + chr4_1:58037493-58037512 None:intergenic 45.0%
TTGTTTGGTTGTTTGGGAGC+TGG - chr4_1:58037492-58037511 Msa0559010:CDS 45.0%
TGTTTGGTTGTTTGGGAGCT+GGG - chr4_1:58037493-58037512 Msa0559010:CDS 45.0%
TTGTTTGGGAGCTGGGAATT+AGG - chr4_1:58037500-58037519 Msa0559010:CDS 45.0%
! GCATAGACACACTTTGGAAG+AGG - chr4_1:58037530-58037549 Msa0559010:CDS 45.0%
!!! TTTTAAGTTATTAATATTTT+AGG + chr4_1:58034741-58034760 None:intergenic 5.0%
!!! ATAATTTTTTTTTAATGATT+TGG + chr4_1:58035613-58035632 None:intergenic 5.0%
TCACACAGCACTTGCCACAT+GGG + chr4_1:58034685-58034704 None:intergenic 50.0%
!! CCACAAATTTGGCTTGAGGG+TGG + chr4_1:58035092-58035111 None:intergenic 50.0%
CCACCCTCAAGCCAAATTTG+TGG - chr4_1:58035089-58035108 Msa0559010:intron 50.0%
GTCCACAGTTCTCCGAAGAT+AGG - chr4_1:58035880-58035899 Msa0559010:intron 50.0%
GTGTACCCCTGTGCTTACTA+TGG + chr4_1:58036590-58036609 None:intergenic 50.0%
! TGATGACTTGGAAGGCGACA+AGG - chr4_1:58036726-58036745 Msa0559010:intron 50.0%
TTGTCTCAATGCTGCACGGA+TGG - chr4_1:58037128-58037147 Msa0559010:CDS 50.0%
GTCACACAGCACTTGCCACA+TGG + chr4_1:58034686-58034705 None:intergenic 55.0%
TGGTCAATGGCATGTCTCCC+AGG - chr4_1:58035858-58035877 Msa0559010:intron 55.0%
CTTCGGAGAACTGTGGACCT+GGG + chr4_1:58035878-58035897 None:intergenic 55.0%
TCTTCGGAGAACTGTGGACC+TGG + chr4_1:58035879-58035898 None:intergenic 55.0%
GTTTGGACGCAGATGGGACT+TGG - chr4_1:58036072-58036091 Msa0559010:intron 55.0%
CAAGGACATCGGACACGACA+CGG - chr4_1:58036282-58036301 Msa0559010:CDS 55.0%
GACTTGGAAGGCGACAAGGT+AGG - chr4_1:58036730-58036749 Msa0559010:intron 55.0%
GTGCTTGGGACTTGCGAGTT+AGG - chr4_1:58037450-58037469 Msa0559010:CDS 55.0%
! CAAATGGCGGAACCCCTTCC+CGG - chr4_1:58035422-58035441 Msa0559010:CDS 60.0%
CTAGAGCTCCCGCATACGCA+GGG + chr4_1:58035449-58035468 None:intergenic 60.0%
ACTAGAGCTCCCGCATACGC+AGG + chr4_1:58035450-58035469 None:intergenic 60.0%
CATACGCAGGGTCCGGGAAG+GGG + chr4_1:58035437-58035456 None:intergenic 65.0%
GCATACGCAGGGTCCGGGAA+GGG + chr4_1:58035438-58035457 None:intergenic 65.0%
CTTCCCGGACCCTGCGTATG+CGG - chr4_1:58035437-58035456 Msa0559010:CDS 65.0%
TTCCCGGACCCTGCGTATGC+GGG - chr4_1:58035438-58035457 Msa0559010:CDS 65.0%
CGCATACGCAGGGTCCGGGA+AGG + chr4_1:58035439-58035458 None:intergenic 70.0%
CTCCCGCATACGCAGGGTCC+GGG + chr4_1:58035443-58035462 None:intergenic 70.0%
GCTCCCGCATACGCAGGGTC+CGG + chr4_1:58035444-58035463 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 58033719 58037692 58033719 ID=Msa0559010;Name=Msa0559010
chr4_1 mRNA 58033719 58037692 58033719 ID=Msa0559010-mRNA-1;Parent=Msa0559010;Name=Msa0559010-mRNA-1;_AED=0.06;_eAED=0.06;_QI=100|1|1|1|0.85|0.75|8|424|565
chr4_1 exon 58033719 58033930 58033719 ID=Msa0559010-mRNA-1:exon:17668;Parent=Msa0559010-mRNA-1
chr4_1 exon 58033978 58034459 58033978 ID=Msa0559010-mRNA-1:exon:17667;Parent=Msa0559010-mRNA-1
chr4_1 exon 58034664 58034912 58034664 ID=Msa0559010-mRNA-1:exon:17666;Parent=Msa0559010-mRNA-1
chr4_1 exon 58035189 58035327 58035189 ID=Msa0559010-mRNA-1:exon:17665;Parent=Msa0559010-mRNA-1
chr4_1 exon 58035405 58035623 58035405 ID=Msa0559010-mRNA-1:exon:17664;Parent=Msa0559010-mRNA-1
chr4_1 exon 58036137 58036503 58036137 ID=Msa0559010-mRNA-1:exon:17663;Parent=Msa0559010-mRNA-1
chr4_1 exon 58037034 58037310 58037034 ID=Msa0559010-mRNA-1:exon:17662;Parent=Msa0559010-mRNA-1
chr4_1 exon 58037416 58037692 58037416 ID=Msa0559010-mRNA-1:exon:17661;Parent=Msa0559010-mRNA-1
chr4_1 five_prime_UTR 58037593 58037692 58037593 ID=Msa0559010-mRNA-1:five_prime_utr;Parent=Msa0559010-mRNA-1
chr4_1 CDS 58037416 58037592 58037416 ID=Msa0559010-mRNA-1:cds;Parent=Msa0559010-mRNA-1
chr4_1 CDS 58037034 58037310 58037034 ID=Msa0559010-mRNA-1:cds;Parent=Msa0559010-mRNA-1
chr4_1 CDS 58036137 58036503 58036137 ID=Msa0559010-mRNA-1:cds;Parent=Msa0559010-mRNA-1
chr4_1 CDS 58035405 58035623 58035405 ID=Msa0559010-mRNA-1:cds;Parent=Msa0559010-mRNA-1
chr4_1 CDS 58035189 58035327 58035189 ID=Msa0559010-mRNA-1:cds;Parent=Msa0559010-mRNA-1
chr4_1 CDS 58034664 58034912 58034664 ID=Msa0559010-mRNA-1:cds;Parent=Msa0559010-mRNA-1
chr4_1 CDS 58034190 58034459 58034190 ID=Msa0559010-mRNA-1:cds;Parent=Msa0559010-mRNA-1
chr4_1 three_prime_UTR 58033978 58034189 58033978 ID=Msa0559010-mRNA-1:three_prime_utr;Parent=Msa0559010-mRNA-1
chr4_1 three_prime_UTR 58033719 58033930 58033719 ID=Msa0559010-mRNA-1:three_prime_utr;Parent=Msa0559010-mRNA-1
Gene Sequence

>Msa0559010

ATGGCTTTTTCTCTTTCTTTCGCTGCTTCAACCAATCATTCAACATTTTCTTCAAACAAAAGCACAAATATTTTGGATATATCTATACCCAAACATGCCTATTCTAGTTTCTATAATAAAACCAATTTAACTACTGTCTTGAATCAAATTCATACTCCTAAAAGTGGAAAATGCAAGGATGATCTTCATATAAGACATGCAAAAGCATTGGATGAACTTAAGCAGGTATTTGTTAGAAATATAAGAAAAAATACAGACGAATGTTTATCTATGGTGGATTCCATCAAAAGATTAGGCATGGAGTACAATTTTGAAGAGGAAATTGAAGCAACACTTGAAAGGAAACACACCATGCTTAGATTTAAGAGTTTCCAGAGAAATGAATATCAAGGACTGTCACAAGCTGCATTTCAATTTCGTATGTTGAGACAAGAAGGGTATTACATTAGCCCAGACATATTTGATAAGTTTTGTGACAACAAAGGAAAACTCAAGCATACATTTTCTGAGGATATAAATGGATTAATGGCCTTGTTTGAAGCTTCTCAATTGAGCATAGAAGGAGAAGACTGTCTTGACAATGTAGGACAGTTCTGCCACCAATATCTTAATGCCTGGTCATCAACATTTCATGGCCACCCTCAAGCCAAATTTGTGGCACACACCTTGATGTATCCAATTCATAAAACTTTGTCAAGATTCACACCAACAATAATGCAATCACAAAATGCAGCATGGACAAACTCTATACAACAATTTTCAGAAATAGACACTCAAATGGTTTCATCATCACATCTCAAGGAAATTTTTGCAGTTTCCAAATGGTGGAAAGACTTAGGTTTGGCAAAGGACTTGGAATTTGCTAGAGATGAACCAATTAAATGGTACTCGTGGTCAATGGCATGTCTCCCAGGTCCACAGTTCTCCGAAGATAGGATTGAGCTCACAAAACCTCTGTCCCTAATATACATCATTGATGATATATTTGATTTTTATGGAAACATTGAAGAACTTACTCTCTTTACAGATGCTGTTGAGAGATGGGACTTGGCAGCTATTGAACAATTACCAGACTGCATGAAAGTGTGTTTCAAGGCTCTCTATGACATAACCAATGAATTTGCTCTTAGGACATACATCAAACATGGATGGAATCCTTTAACCTCATTAATAAAATCGTGGGTAAGACTCTTGAATGCTTTCTTGCAAGAAGCAAAATGGTTTGCTTCTGGGAACGTTCCAAATTCAGAGGAGTATTTGAAGAATGCCATAGTAAGCACAGGGGTACACGTGATACTTGTGCATGCTTTCTTTTGCATGGGTCAAGGTATAACTGAGAAAACCGTGTCTCTAATGGATGACTTCCCAACCATTATATCTACAACAGCCAAAATTCTAAGGCTATGTGATGACTTGGAAGGCGACAAGGATGTTAATTGTGAGGGTAATGATGGGTCATATTCGAAGTGTTACATGAAAGATAACCCAGGAGTTTCTATTGGACAAACAAAGGAGCATATGAGTCAACAAATTTCAGATGCATGGAAACAACTCAACAAGGAATGTTTGAACACAAATCCATTACCATCATCTTTTACTAAACTTTGTCTCAATGCTGCACGGATGGTACCTATAATGTACAATTATGATGGCAATACTTCTTCAAAGCTAGAGGAGTATGTGAAATCGTTGCTTTAG
Protein sequence

>Msa0559010

MAFSLSFAASTNHSTFSSNKSTNILDISIPKHAYSSFYNKTNLTTVLNQIHTPKSGKCKDDLHIRHAKALDELKQVFVRNIRKNTDECLSMVDSIKRLGMEYNFEEEIEATLERKHTMLRFKSFQRNEYQGLSQAAFQFRMLRQEGYYISPDIFDKFCDNKGKLKHTFSEDINGLMALFEASQLSIEGEDCLDNVGQFCHQYLNAWSSTFHGHPQAKFVAHTLMYPIHKTLSRFTPTIMQSQNAAWTNSIQQFSEIDTQMVSSSHLKEIFAVSKWWKDLGLAKDLEFARDEPIKWYSWSMACLPGPQFSEDRIELTKPLSLIYIIDDIFDFYGNIEELTLFTDAVERWDLAAIEQLPDCMKVCFKALYDITNEFALRTYIKHGWNPLTSLIKSWVRLLNAFLQEAKWFASGNVPNSEEYLKNAIVSTGVHVILVHAFFCMGQGITEKTVSLMDDFPTIISTTAKILRLCDDLEGDKDVNCEGNDGSYSKCYMKDNPGVSIGQTKEHMSQQISDAWKQLNKECLNTNPLPSSFTKLCLNAARMVPIMYNYDGNTSSKLEEYVKSLL*