AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0654600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0654600 MtrunA17_Chr4g0049241 95.197 229 11 0 13 241 176 404 1.17e-148 420
Msa0654600 MtrunA17_Chr3g0135321 56.596 235 98 2 9 241 155 387 7.33e-90 270
Msa0654600 MtrunA17_Chr4g0049231 53.744 227 101 2 17 241 133 357 8.37e-83 251
Msa0654600 MtrunA17_Chr5g0400811 53.913 230 102 2 13 240 180 407 8.56e-81 248
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0654600 AT1G50110.2 61.739 230 85 2 13 241 90 317 1.17e-100 296
Msa0654600 AT1G50110.1 61.739 230 85 2 13 241 125 352 4.24e-100 295
Msa0654600 AT1G50090.1 59.130 230 91 2 13 241 128 355 2.56e-95 284
Msa0654600 AT1G10070.3 57.021 235 97 2 9 241 86 318 3.50e-95 281
Msa0654600 AT1G10070.1 57.021 235 97 2 9 241 156 388 2.29e-94 282
Msa0654600 AT1G10070.2 57.021 235 97 2 9 241 156 388 2.29e-94 282
Msa0654600 AT1G10060.3 58.261 230 92 2 13 240 90 317 7.51e-91 271
Msa0654600 AT1G10060.2 58.261 230 92 2 13 240 156 383 6.05e-90 271
Msa0654600 AT1G10060.5 58.261 230 92 2 13 240 252 479 8.49e-89 271
Msa0654600 AT3G19710.1 53.846 234 105 2 9 241 120 351 3.46e-88 265
Msa0654600 AT3G49680.2 56.277 231 96 3 13 240 182 410 3.31e-85 259
Msa0654600 AT3G49680.1 56.277 231 96 3 13 240 184 412 3.90e-85 259
Msa0654600 AT5G65780.1 55.411 231 98 3 13 240 186 414 1.16e-83 255
Msa0654600 AT1G10060.7 58.974 195 76 2 13 205 156 348 8.20e-74 229
Msa0654600 AT1G10060.4 59.487 195 75 2 13 205 252 444 1.01e-72 229
Msa0654600 AT1G10060.1 55.944 143 59 2 13 153 156 296 7.66e-48 160
Msa0654600 AT1G10060.6 55.944 143 59 2 13 153 252 392 7.51e-47 160

Find 71 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCATTGATTTGGGTTATA+AGG 0.149101 4_3:-62776184 Msa0654600:intron
TAAGTTCAGTTTATTATAAA+TGG 0.157123 4_3:+62775516 None:intergenic
GTTTATTATAAATGGAAATT+TGG 0.163762 4_3:+62775524 None:intergenic
GTCCAAGTAGATTGAGAAAT+TGG 0.258521 4_3:-62775688 Msa0654600:exon
CATAGACATCGCCATTGATT+TGG 0.259021 4_3:-62776193 Msa0654600:CDS
CTAATCATCAATAGTATGTT+TGG 0.287341 4_3:-62775622 Msa0654600:three_prime_UTR
TGTTGCATCTGTAACATATA+AGG 0.310394 4_3:-62775962 Msa0654600:CDS
CATCATTTCCTCCACTGATA+TGG 0.317405 4_3:+62776032 None:intergenic
GGAAAGGGACGCTTTATCTT+AGG 0.336223 4_3:-62777078 Msa0654600:CDS
CAAACATACTATTGATGATT+AGG 0.337825 4_3:+62775623 None:intergenic
ATCTTCACTCCGGCAATAGA+TGG 0.342656 4_3:-62776249 Msa0654600:CDS
TATCTTAGGCCTTTGCTGAT+AGG 0.349392 4_3:-62777064 Msa0654600:CDS
TTGTTCTTGGATTCAGCAAC+TGG 0.355547 4_3:-62776462 Msa0654600:CDS
ATAGACATCGCCATTGATTT+GGG 0.368721 4_3:-62776192 Msa0654600:CDS
TGAGCTTTCAAAATTGAAAC+AGG 0.392634 4_3:-62777113 Msa0654600:CDS
ACCTCGTGGTAATTTCCAAC+AGG 0.408370 4_3:+62776980 None:intergenic
AGGCCTTTGCTGATAGGAAC+AGG 0.426821 4_3:-62777058 Msa0654600:CDS
TGCCAATTTCTCAATCTACT+TGG 0.427171 4_3:+62775686 None:intergenic
ATACTGGTTGGAATACAAAC+AGG 0.434484 4_3:-62775739 Msa0654600:CDS
ATAGCTGGCAGTGGTGGAAC+AGG 0.460699 4_3:-62776791 Msa0654600:CDS
AGAATAGATCCATCTATTGC+CGG 0.463647 4_3:+62776240 None:intergenic
CTCATTTATTGCTCCCCTGT+TGG 0.463936 4_3:-62776995 Msa0654600:CDS
TTGAAACAGGTACCTCCTCC+AGG 0.466899 4_3:-62777100 Msa0654600:CDS
TGCAGAGCTGAATATAAAAC+AGG 0.474986 4_3:-62775793 Msa0654600:intron
ATCAATGGCGATGTCTATGA+TGG 0.477143 4_3:+62776197 None:intergenic
GATTTCAGTTGCAAACATAC+AGG 0.481875 4_3:-62775600 Msa0654600:three_prime_UTR
AGATAAAGCGTCCCTTTCCC+TGG 0.488127 4_3:+62777082 None:intergenic
ATAGATGGATCTATTCTGCC+TGG 0.500850 4_3:-62776234 Msa0654600:CDS
TACCTTATAACCCAAATCAA+TGG 0.507754 4_3:+62776182 None:intergenic
TATAAATGGAAATTTGGACT+TGG 0.509183 4_3:+62775530 None:intergenic
AGTTGCAAACATACAGGTAG+AGG 0.514094 4_3:-62775594 Msa0654600:three_prime_UTR
ATAGGAACAGGAGCTGCATT+AGG 0.514522 4_3:-62777046 Msa0654600:CDS
AACAGGAGCTGCATTAGGCT+TGG 0.520575 4_3:-62777041 Msa0654600:CDS
TAGATGGATCTATTCTGCCT+GGG 0.527089 4_3:-62776233 Msa0654600:CDS
CGATCAATAGCTGGCAGTGG+TGG 0.529542 4_3:-62776797 Msa0654600:CDS
AGGTACCTCCTCCAGGGAAA+GGG 0.530899 4_3:-62777093 Msa0654600:CDS
AAACTACGAGGAATACTGGT+TGG 0.534155 4_3:-62775751 Msa0654600:CDS
CAGGTACCTCCTCCAGGGAA+AGG 0.544439 4_3:-62777094 Msa0654600:CDS
AAATTTCATCGATCAATAGC+TGG 0.549508 4_3:-62776806 Msa0654600:CDS
AGGAAGACTAAACTTAAAAG+TGG 0.553988 4_3:-62776834 Msa0654600:CDS
AGCGTCCCTTTCCCTGGAGG+AGG 0.558749 4_3:+62777088 None:intergenic
CTGTAACATATAAGGAAACA+AGG 0.572784 4_3:-62775954 Msa0654600:intron
CAAGACATCAGAAAATCCAT+TGG 0.572958 4_3:+62776482 None:intergenic
TAAATGAGAAATGTGTACTC+AGG 0.581598 4_3:+62777010 None:intergenic
GTAACTGAAGCAAAAGCCAA+TGG 0.582493 4_3:-62776498 Msa0654600:CDS
ATGCAATATATTTGTTGTGA+AGG 0.592887 4_3:-62776415 Msa0654600:intron
GGAAAATGATATCTTCACTC+CGG 0.596052 4_3:-62776259 Msa0654600:CDS
AATACAAACAGGGCGTATTG+AGG 0.599236 4_3:-62775728 Msa0654600:CDS
AGCAACTGGAAACAATATTG+AGG 0.599305 4_3:-62776448 Msa0654600:CDS
GCTGGCAGTGGTGGAACAGG+AGG 0.602903 4_3:-62776788 Msa0654600:CDS
GTGTGTGAATCTGCTTTGTG+TGG 0.604347 4_3:-62775468 Msa0654600:three_prime_UTR
CATCGATCAATAGCTGGCAG+TGG 0.604641 4_3:-62776800 Msa0654600:CDS
CATAGAACGTTCCATATCAG+TGG 0.605813 4_3:-62776043 Msa0654600:CDS
AGAAATGTGTACTCAGGTGA+TGG 0.612357 4_3:+62777016 None:intergenic
GCTGATGAAGTGTTCTGCAC+AGG 0.613446 4_3:-62776006 Msa0654600:CDS
GCTCCTGTTCCTATCAGCAA+AGG 0.618333 4_3:+62777055 None:intergenic
AAGACTAAACTTAAAAGTGG+AGG 0.620669 4_3:-62776831 Msa0654600:CDS
GAGTGTTACTAATTATGCCC+CGG 0.621088 4_3:-62776759 Msa0654600:intron
CCTCGTGGTAATTTCCAACA+GGG 0.633692 4_3:+62776981 None:intergenic
TGAAACAGGTACCTCCTCCA+GGG 0.635669 4_3:-62777099 Msa0654600:CDS
AGAACGTTCCATATCAGTGG+AGG 0.645297 4_3:-62776040 Msa0654600:CDS
AACTGGAAACAATATTGAGG+AGG 0.649999 4_3:-62776445 Msa0654600:CDS
TTCCAAGCTACTGGCTACAA+CGG 0.650241 4_3:+62775415 None:intergenic
AGATGGATCTATTCTGCCTG+GGG 0.660994 4_3:-62776232 Msa0654600:CDS
ACAAGTATTATCATTTAACA+AGG 0.662636 4_3:+62775660 None:intergenic
TAAAGCGTCCCTTTCCCTGG+AGG 0.677703 4_3:+62777085 None:intergenic
ATATGTTACAGATGCAACAG+AGG 0.681902 4_3:+62775966 None:intergenic
TACTGGTTGGAATACAAACA+GGG 0.699480 4_3:-62775738 Msa0654600:CDS
CTCGTGGTAATTTCCAACAG+GGG 0.700556 4_3:+62776982 None:intergenic
CCCTGTTGGAAATTACCACG+AGG 0.728651 4_3:-62776981 Msa0654600:intron
GTTCTGCACAGGAACTGCAG+TGG 0.751564 4_3:-62775995 Msa0654600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGGAAAATATATAATAATT+TGG + chr4_3:62775898-62775917 None:intergenic 10.0%
!!! AAATTATTATATATTTTCCT+TGG - chr4_3:62775897-62775916 Msa0654600:intron 10.0%
!! AAGGAAAATATATAATAATT+TGG + chr4_3:62775898-62775917 None:intergenic 10.0%
!!! AAATTATTATATATTTTCCT+TGG - chr4_3:62775897-62775916 Msa0654600:intron 10.0%
!!! CTTATATTGTTTTGTTAATA+AGG - chr4_3:62775633-62775652 Msa0654600:three_prime_UTR 15.0%
!! TGTAAATTTGTAGAAAATAA+TGG + chr4_3:62776438-62776457 None:intergenic 15.0%
!! TATAGATGATTAATGATTTA+TGG - chr4_3:62776459-62776478 Msa0654600:CDS 15.0%
!! AAAAATTGACTAAAATGAAA+GGG + chr4_3:62776697-62776716 None:intergenic 15.0%
!! AAAAAATTGACTAAAATGAA+AGG + chr4_3:62776698-62776717 None:intergenic 15.0%
!!! TTTTAGTCAATTTTTTTTGT+TGG - chr4_3:62776702-62776721 Msa0654600:intron 15.0%
!! GTTTATTATAAATGGAAATT+TGG + chr4_3:62777049-62777068 None:intergenic 15.0%
!! TAAGTTCAGTTTATTATAAA+TGG + chr4_3:62777057-62777076 None:intergenic 15.0%
!!! CTTATATTGTTTTGTTAATA+AGG - chr4_3:62775633-62775652 Msa0654600:three_prime_UTR 15.0%
!! TGTAAATTTGTAGAAAATAA+TGG + chr4_3:62776438-62776457 None:intergenic 15.0%
!! TATAGATGATTAATGATTTA+TGG - chr4_3:62776459-62776478 Msa0654600:CDS 15.0%
!! AAAAATTGACTAAAATGAAA+GGG + chr4_3:62776697-62776716 None:intergenic 15.0%
!! AAAAAATTGACTAAAATGAA+AGG + chr4_3:62776698-62776717 None:intergenic 15.0%
!!! TTTTAGTCAATTTTTTTTGT+TGG - chr4_3:62776702-62776721 Msa0654600:intron 15.0%
!! GTTTATTATAAATGGAAATT+TGG + chr4_3:62777049-62777068 None:intergenic 15.0%
!! TAAGTTCAGTTTATTATAAA+TGG + chr4_3:62777057-62777076 None:intergenic 15.0%
!! AAAAAAAAAAAATAACAGGG+AGG - chr4_3:62775716-62775735 Msa0654600:CDS 20.0%
!! ACAAGTATTATCATTTAACA+AGG + chr4_3:62776913-62776932 None:intergenic 20.0%
!! AAAAAAAAAAAATAACAGGG+AGG - chr4_3:62775716-62775735 Msa0654600:CDS 20.0%
!! ACAAGTATTATCATTTAACA+AGG + chr4_3:62776913-62776932 None:intergenic 20.0%
! TATAAGTAAATCCAAATCCA+TGG + chr4_3:62775619-62775638 None:intergenic 25.0%
!!! ATGCAATATATTTGTTGTGA+AGG - chr4_3:62776155-62776174 Msa0654600:intron 25.0%
! GTTTCGGATATAGTAAAATA+TGG + chr4_3:62776249-62776268 None:intergenic 25.0%
! ACTATATCCGAAACAAAAAT+TGG - chr4_3:62776255-62776274 Msa0654600:CDS 25.0%
!!! CATTAGACCAATTTTTGTTT+CGG + chr4_3:62776265-62776284 None:intergenic 25.0%
!! GTTTTGCATTAATCTTGTTA+AGG - chr4_3:62776290-62776309 Msa0654600:intron 25.0%
! AGAAAATAATGGCAAACATT+GGG + chr4_3:62776427-62776446 None:intergenic 25.0%
! TAGAAAATAATGGCAAACAT+TGG + chr4_3:62776428-62776447 None:intergenic 25.0%
! TTTGTAACAAAACTATGTTC+AGG - chr4_3:62776503-62776522 Msa0654600:CDS 25.0%
! TTGTGTTTAGCATTATCATT+AGG + chr4_3:62776742-62776761 None:intergenic 25.0%
!! TAATGATAATGCTAAACACA+AGG - chr4_3:62776741-62776760 Msa0654600:intron 25.0%
!! CAAACATACTATTGATGATT+AGG + chr4_3:62776950-62776969 None:intergenic 25.0%
! CTAATCATCAATAGTATGTT+TGG - chr4_3:62776948-62776967 Msa0654600:intron 25.0%
! TATAAATGGAAATTTGGACT+TGG + chr4_3:62777043-62777062 None:intergenic 25.0%
! TATAAGTAAATCCAAATCCA+TGG + chr4_3:62775619-62775638 None:intergenic 25.0%
!!! ATGCAATATATTTGTTGTGA+AGG - chr4_3:62776155-62776174 Msa0654600:intron 25.0%
! GTTTCGGATATAGTAAAATA+TGG + chr4_3:62776249-62776268 None:intergenic 25.0%
! ACTATATCCGAAACAAAAAT+TGG - chr4_3:62776255-62776274 Msa0654600:CDS 25.0%
!!! CATTAGACCAATTTTTGTTT+CGG + chr4_3:62776265-62776284 None:intergenic 25.0%
!! GTTTTGCATTAATCTTGTTA+AGG - chr4_3:62776290-62776309 Msa0654600:intron 25.0%
! AGAAAATAATGGCAAACATT+GGG + chr4_3:62776427-62776446 None:intergenic 25.0%
! TAGAAAATAATGGCAAACAT+TGG + chr4_3:62776428-62776447 None:intergenic 25.0%
! TTTGTAACAAAACTATGTTC+AGG - chr4_3:62776503-62776522 Msa0654600:CDS 25.0%
! TTGTGTTTAGCATTATCATT+AGG + chr4_3:62776742-62776761 None:intergenic 25.0%
!! TAATGATAATGCTAAACACA+AGG - chr4_3:62776741-62776760 Msa0654600:intron 25.0%
!! CAAACATACTATTGATGATT+AGG + chr4_3:62776950-62776969 None:intergenic 25.0%
! CTAATCATCAATAGTATGTT+TGG - chr4_3:62776948-62776967 Msa0654600:intron 25.0%
! TATAAATGGAAATTTGGACT+TGG + chr4_3:62777043-62777062 None:intergenic 25.0%
TGAGCTTTCAAAATTGAAAC+AGG - chr4_3:62775457-62775476 Msa0654600:three_prime_UTR 30.0%
TAAATGAGAAATGTGTACTC+AGG + chr4_3:62775563-62775582 None:intergenic 30.0%
AGGAAGACTAAACTTAAAAG+TGG - chr4_3:62775736-62775755 Msa0654600:CDS 30.0%
AAGACTAAACTTAAAAGTGG+AGG - chr4_3:62775739-62775758 Msa0654600:CDS 30.0%
AAATTTCATCGATCAATAGC+TGG - chr4_3:62775764-62775783 Msa0654600:CDS 30.0%
TTATATACATCACCGTTTGA+TGG - chr4_3:62775862-62775881 Msa0654600:intron 30.0%
GGAGAAAAAAAACCATCAAA+CGG + chr4_3:62775877-62775896 None:intergenic 30.0%
TTGAGAAGAAATCACATCAA+AGG - chr4_3:62775946-62775965 Msa0654600:intron 30.0%
! ACTGATGCATATTGTTACTT+AGG - chr4_3:62775969-62775988 Msa0654600:CDS 30.0%
CTCTTCATTATGCATATTTC+TGG - chr4_3:62776029-62776048 Msa0654600:CDS 30.0%
!! ATTTTCTGATGTCTTGTTCT+TGG - chr4_3:62776095-62776114 Msa0654600:intron 30.0%
TACCTTATAACCCAAATCAA+TGG + chr4_3:62776391-62776410 None:intergenic 30.0%
TGTTGCATCTGTAACATATA+AGG - chr4_3:62776608-62776627 Msa0654600:intron 30.0%
CTGTAACATATAAGGAAACA+AGG - chr4_3:62776616-62776635 Msa0654600:intron 30.0%
TGAGCTTTCAAAATTGAAAC+AGG - chr4_3:62775457-62775476 Msa0654600:three_prime_UTR 30.0%
TAAATGAGAAATGTGTACTC+AGG + chr4_3:62775563-62775582 None:intergenic 30.0%
AGGAAGACTAAACTTAAAAG+TGG - chr4_3:62775736-62775755 Msa0654600:CDS 30.0%
AAGACTAAACTTAAAAGTGG+AGG - chr4_3:62775739-62775758 Msa0654600:CDS 30.0%
AAATTTCATCGATCAATAGC+TGG - chr4_3:62775764-62775783 Msa0654600:CDS 30.0%
TTATATACATCACCGTTTGA+TGG - chr4_3:62775862-62775881 Msa0654600:intron 30.0%
GGAGAAAAAAAACCATCAAA+CGG + chr4_3:62775877-62775896 None:intergenic 30.0%
TTGAGAAGAAATCACATCAA+AGG - chr4_3:62775946-62775965 Msa0654600:intron 30.0%
! ACTGATGCATATTGTTACTT+AGG - chr4_3:62775969-62775988 Msa0654600:CDS 30.0%
CTCTTCATTATGCATATTTC+TGG - chr4_3:62776029-62776048 Msa0654600:CDS 30.0%
!! ATTTTCTGATGTCTTGTTCT+TGG - chr4_3:62776095-62776114 Msa0654600:intron 30.0%
TACCTTATAACCCAAATCAA+TGG + chr4_3:62776391-62776410 None:intergenic 30.0%
TGTTGCATCTGTAACATATA+AGG - chr4_3:62776608-62776627 Msa0654600:intron 30.0%
CTGTAACATATAAGGAAACA+AGG - chr4_3:62776616-62776635 Msa0654600:intron 30.0%
CAAGACATCAGAAAATCCAT+TGG + chr4_3:62776091-62776110 None:intergenic 35.0%
AGCAACTGGAAACAATATTG+AGG - chr4_3:62776122-62776141 Msa0654600:intron 35.0%
AACTGGAAACAATATTGAGG+AGG - chr4_3:62776125-62776144 Msa0654600:intron 35.0%
GGAAAATGATATCTTCACTC+CGG - chr4_3:62776311-62776330 Msa0654600:intron 35.0%
AGAATAGATCCATCTATTGC+CGG + chr4_3:62776333-62776352 None:intergenic 35.0%
!! ATAGACATCGCCATTGATTT+GGG - chr4_3:62776378-62776397 Msa0654600:intron 35.0%
AATAATGGCAAACATTGGGA+GGG + chr4_3:62776423-62776442 None:intergenic 35.0%
AAATAATGGCAAACATTGGG+AGG + chr4_3:62776424-62776443 None:intergenic 35.0%
ATATGTTACAGATGCAACAG+AGG + chr4_3:62776607-62776626 None:intergenic 35.0%
TGCAGAGCTGAATATAAAAC+AGG - chr4_3:62776777-62776796 Msa0654600:CDS 35.0%
TGAAAAACTACGAGGAATAC+TGG - chr4_3:62776815-62776834 Msa0654600:CDS 35.0%
! ATACTGGTTGGAATACAAAC+AGG - chr4_3:62776831-62776850 Msa0654600:CDS 35.0%
! TACTGGTTGGAATACAAACA+GGG - chr4_3:62776832-62776851 Msa0654600:CDS 35.0%
GTATTGAGGACAAAAAGTCA+TGG - chr4_3:62776856-62776875 Msa0654600:intron 35.0%
GTCCAAGTAGATTGAGAAAT+TGG - chr4_3:62776882-62776901 Msa0654600:intron 35.0%
TGCCAATTTCTCAATCTACT+TGG + chr4_3:62776887-62776906 None:intergenic 35.0%
GATTTCAGTTGCAAACATAC+AGG - chr4_3:62776970-62776989 Msa0654600:intron 35.0%
CAAGACATCAGAAAATCCAT+TGG + chr4_3:62776091-62776110 None:intergenic 35.0%
AGCAACTGGAAACAATATTG+AGG - chr4_3:62776122-62776141 Msa0654600:intron 35.0%
AACTGGAAACAATATTGAGG+AGG - chr4_3:62776125-62776144 Msa0654600:intron 35.0%
GGAAAATGATATCTTCACTC+CGG - chr4_3:62776311-62776330 Msa0654600:intron 35.0%
AGAATAGATCCATCTATTGC+CGG + chr4_3:62776333-62776352 None:intergenic 35.0%
!! ATAGACATCGCCATTGATTT+GGG - chr4_3:62776378-62776397 Msa0654600:intron 35.0%
AATAATGGCAAACATTGGGA+GGG + chr4_3:62776423-62776442 None:intergenic 35.0%
AAATAATGGCAAACATTGGG+AGG + chr4_3:62776424-62776443 None:intergenic 35.0%
ATATGTTACAGATGCAACAG+AGG + chr4_3:62776607-62776626 None:intergenic 35.0%
TGCAGAGCTGAATATAAAAC+AGG - chr4_3:62776777-62776796 Msa0654600:CDS 35.0%
TGAAAAACTACGAGGAATAC+TGG - chr4_3:62776815-62776834 Msa0654600:CDS 35.0%
! ATACTGGTTGGAATACAAAC+AGG - chr4_3:62776831-62776850 Msa0654600:CDS 35.0%
! TACTGGTTGGAATACAAACA+GGG - chr4_3:62776832-62776851 Msa0654600:CDS 35.0%
GTATTGAGGACAAAAAGTCA+TGG - chr4_3:62776856-62776875 Msa0654600:intron 35.0%
GTCCAAGTAGATTGAGAAAT+TGG - chr4_3:62776882-62776901 Msa0654600:intron 35.0%
TGCCAATTTCTCAATCTACT+TGG + chr4_3:62776887-62776906 None:intergenic 35.0%
GATTTCAGTTGCAAACATAC+AGG - chr4_3:62776970-62776989 Msa0654600:intron 35.0%
TATCTTAGGCCTTTGCTGAT+AGG - chr4_3:62775506-62775525 Msa0654600:three_prime_UTR 40.0%
AGAAATGTGTACTCAGGTGA+TGG + chr4_3:62775557-62775576 None:intergenic 40.0%
! GAGTGTTACTAATTATGCCC+CGG - chr4_3:62775811-62775830 Msa0654600:intron 40.0%
ATCACTATTGCCATGCTTAC+CGG + chr4_3:62775833-62775852 None:intergenic 40.0%
! CTTTTCACAAGTCACAACCA+AGG + chr4_3:62775917-62775936 None:intergenic 40.0%
GTAACTGAAGCAAAAGCCAA+TGG - chr4_3:62776072-62776091 Msa0654600:intron 40.0%
TTGTTCTTGGATTCAGCAAC+TGG - chr4_3:62776108-62776127 Msa0654600:intron 40.0%
ATAGATGGATCTATTCTGCC+TGG - chr4_3:62776336-62776355 Msa0654600:intron 40.0%
TAGATGGATCTATTCTGCCT+GGG - chr4_3:62776337-62776356 Msa0654600:intron 40.0%
ATCAATGGCGATGTCTATGA+TGG + chr4_3:62776376-62776395 None:intergenic 40.0%
! CATAGACATCGCCATTGATT+TGG - chr4_3:62776377-62776396 Msa0654600:intron 40.0%
!! CGCCATTGATTTGGGTTATA+AGG - chr4_3:62776386-62776405 Msa0654600:intron 40.0%
CATAGAACGTTCCATATCAG+TGG - chr4_3:62776527-62776546 Msa0654600:intron 40.0%
CATCATTTCCTCCACTGATA+TGG + chr4_3:62776541-62776560 None:intergenic 40.0%
ACGTTGTCTGAAAAACTACG+AGG - chr4_3:62776807-62776826 Msa0654600:CDS 40.0%
AAACTACGAGGAATACTGGT+TGG - chr4_3:62776819-62776838 Msa0654600:CDS 40.0%
AATACAAACAGGGCGTATTG+AGG - chr4_3:62776842-62776861 Msa0654600:intron 40.0%
AGTTGCAAACATACAGGTAG+AGG - chr4_3:62776976-62776995 Msa0654600:intron 40.0%
TATCTTAGGCCTTTGCTGAT+AGG - chr4_3:62775506-62775525 Msa0654600:three_prime_UTR 40.0%
AGAAATGTGTACTCAGGTGA+TGG + chr4_3:62775557-62775576 None:intergenic 40.0%
! GAGTGTTACTAATTATGCCC+CGG - chr4_3:62775811-62775830 Msa0654600:intron 40.0%
ATCACTATTGCCATGCTTAC+CGG + chr4_3:62775833-62775852 None:intergenic 40.0%
! CTTTTCACAAGTCACAACCA+AGG + chr4_3:62775917-62775936 None:intergenic 40.0%
GTAACTGAAGCAAAAGCCAA+TGG - chr4_3:62776072-62776091 Msa0654600:intron 40.0%
TTGTTCTTGGATTCAGCAAC+TGG - chr4_3:62776108-62776127 Msa0654600:intron 40.0%
ATAGATGGATCTATTCTGCC+TGG - chr4_3:62776336-62776355 Msa0654600:intron 40.0%
TAGATGGATCTATTCTGCCT+GGG - chr4_3:62776337-62776356 Msa0654600:intron 40.0%
ATCAATGGCGATGTCTATGA+TGG + chr4_3:62776376-62776395 None:intergenic 40.0%
! CATAGACATCGCCATTGATT+TGG - chr4_3:62776377-62776396 Msa0654600:intron 40.0%
!! CGCCATTGATTTGGGTTATA+AGG - chr4_3:62776386-62776405 Msa0654600:intron 40.0%
CATAGAACGTTCCATATCAG+TGG - chr4_3:62776527-62776546 Msa0654600:intron 40.0%
CATCATTTCCTCCACTGATA+TGG + chr4_3:62776541-62776560 None:intergenic 40.0%
ACGTTGTCTGAAAAACTACG+AGG - chr4_3:62776807-62776826 Msa0654600:CDS 40.0%
AAACTACGAGGAATACTGGT+TGG - chr4_3:62776819-62776838 Msa0654600:CDS 40.0%
AATACAAACAGGGCGTATTG+AGG - chr4_3:62776842-62776861 Msa0654600:intron 40.0%
AGTTGCAAACATACAGGTAG+AGG - chr4_3:62776976-62776995 Msa0654600:intron 40.0%
GGAAAGGGACGCTTTATCTT+AGG - chr4_3:62775492-62775511 Msa0654600:three_prime_UTR 45.0%
ATAGGAACAGGAGCTGCATT+AGG - chr4_3:62775524-62775543 Msa0654600:three_prime_UTR 45.0%
CTCATTTATTGCTCCCCTGT+TGG - chr4_3:62775575-62775594 Msa0654600:three_prime_UTR 45.0%
CTCGTGGTAATTTCCAACAG+GGG + chr4_3:62775591-62775610 None:intergenic 45.0%
CCTCGTGGTAATTTCCAACA+GGG + chr4_3:62775592-62775611 None:intergenic 45.0%
ACCTCGTGGTAATTTCCAAC+AGG + chr4_3:62775593-62775612 None:intergenic 45.0%
TAATTATGCCCCGGTAAGCA+TGG - chr4_3:62775820-62775839 Msa0654600:intron 45.0%
TCACTATTGCCATGCTTACC+GGG + chr4_3:62775832-62775851 None:intergenic 45.0%
ATCTTCACTCCGGCAATAGA+TGG - chr4_3:62776321-62776340 Msa0654600:intron 45.0%
AGATGGATCTATTCTGCCTG+GGG - chr4_3:62776338-62776357 Msa0654600:intron 45.0%
AGAACGTTCCATATCAGTGG+AGG - chr4_3:62776530-62776549 Msa0654600:intron 45.0%
GTGTGTGAATCTGCTTTGTG+TGG - chr4_3:62777102-62777121 Msa0654600:CDS 45.0%
GGAAAGGGACGCTTTATCTT+AGG - chr4_3:62775492-62775511 Msa0654600:three_prime_UTR 45.0%
ATAGGAACAGGAGCTGCATT+AGG - chr4_3:62775524-62775543 Msa0654600:three_prime_UTR 45.0%
CTCATTTATTGCTCCCCTGT+TGG - chr4_3:62775575-62775594 Msa0654600:three_prime_UTR 45.0%
CTCGTGGTAATTTCCAACAG+GGG + chr4_3:62775591-62775610 None:intergenic 45.0%
CCTCGTGGTAATTTCCAACA+GGG + chr4_3:62775592-62775611 None:intergenic 45.0%
ACCTCGTGGTAATTTCCAAC+AGG + chr4_3:62775593-62775612 None:intergenic 45.0%
TAATTATGCCCCGGTAAGCA+TGG - chr4_3:62775820-62775839 Msa0654600:intron 45.0%
TCACTATTGCCATGCTTACC+GGG + chr4_3:62775832-62775851 None:intergenic 45.0%
ATCTTCACTCCGGCAATAGA+TGG - chr4_3:62776321-62776340 Msa0654600:intron 45.0%
AGATGGATCTATTCTGCCTG+GGG - chr4_3:62776338-62776357 Msa0654600:intron 45.0%
AGAACGTTCCATATCAGTGG+AGG - chr4_3:62776530-62776549 Msa0654600:intron 45.0%
GTGTGTGAATCTGCTTTGTG+TGG - chr4_3:62777102-62777121 Msa0654600:CDS 45.0%
!!! TTTTTTTAAAAAAAAAAAGA+AGG + chr4_3:62775702-62775721 None:intergenic 5.0%
!! TTTAAAAAAAAAAAAATAAC+AGG - chr4_3:62775712-62775731 Msa0654600:CDS 5.0%
!! TTAAAAAAAAAAAAATAACA+GGG - chr4_3:62775713-62775732 Msa0654600:CDS 5.0%
!!! TTTTTTTAAAAAAAAAAAGA+AGG + chr4_3:62775702-62775721 None:intergenic 5.0%
!! TTTAAAAAAAAAAAAATAAC+AGG - chr4_3:62775712-62775731 Msa0654600:CDS 5.0%
!! TTAAAAAAAAAAAAATAACA+GGG - chr4_3:62775713-62775732 Msa0654600:CDS 5.0%
TTGAAACAGGTACCTCCTCC+AGG - chr4_3:62775470-62775489 Msa0654600:three_prime_UTR 50.0%
TGAAACAGGTACCTCCTCCA+GGG - chr4_3:62775471-62775490 Msa0654600:three_prime_UTR 50.0%
! AGATAAAGCGTCCCTTTCCC+TGG + chr4_3:62775491-62775510 None:intergenic 50.0%
AGGCCTTTGCTGATAGGAAC+AGG - chr4_3:62775512-62775531 Msa0654600:three_prime_UTR 50.0%
GCTCCTGTTCCTATCAGCAA+AGG + chr4_3:62775518-62775537 None:intergenic 50.0%
AACAGGAGCTGCATTAGGCT+TGG - chr4_3:62775529-62775548 Msa0654600:three_prime_UTR 50.0%
CCCTGTTGGAAATTACCACG+AGG - chr4_3:62775589-62775608 Msa0654600:three_prime_UTR 50.0%
AATTACCACGAGGTCAGCCA+TGG - chr4_3:62775599-62775618 Msa0654600:three_prime_UTR 50.0%
CATCGATCAATAGCTGGCAG+TGG - chr4_3:62775770-62775789 Msa0654600:CDS 50.0%
CACTATTGCCATGCTTACCG+GGG + chr4_3:62775831-62775850 None:intergenic 50.0%
! ATGGATTTTCGTGTGACCCC+AGG + chr4_3:62776357-62776376 None:intergenic 50.0%
! GCTGATGAAGTGTTCTGCAC+AGG - chr4_3:62776564-62776583 Msa0654600:intron 50.0%
TTGAAACAGGTACCTCCTCC+AGG - chr4_3:62775470-62775489 Msa0654600:three_prime_UTR 50.0%
TGAAACAGGTACCTCCTCCA+GGG - chr4_3:62775471-62775490 Msa0654600:three_prime_UTR 50.0%
! AGATAAAGCGTCCCTTTCCC+TGG + chr4_3:62775491-62775510 None:intergenic 50.0%
AGGCCTTTGCTGATAGGAAC+AGG - chr4_3:62775512-62775531 Msa0654600:three_prime_UTR 50.0%
GCTCCTGTTCCTATCAGCAA+AGG + chr4_3:62775518-62775537 None:intergenic 50.0%
AACAGGAGCTGCATTAGGCT+TGG - chr4_3:62775529-62775548 Msa0654600:three_prime_UTR 50.0%
CCCTGTTGGAAATTACCACG+AGG - chr4_3:62775589-62775608 Msa0654600:three_prime_UTR 50.0%
AATTACCACGAGGTCAGCCA+TGG - chr4_3:62775599-62775618 Msa0654600:three_prime_UTR 50.0%
CATCGATCAATAGCTGGCAG+TGG - chr4_3:62775770-62775789 Msa0654600:CDS 50.0%
CACTATTGCCATGCTTACCG+GGG + chr4_3:62775831-62775850 None:intergenic 50.0%
! ATGGATTTTCGTGTGACCCC+AGG + chr4_3:62776357-62776376 None:intergenic 50.0%
! GCTGATGAAGTGTTCTGCAC+AGG - chr4_3:62776564-62776583 Msa0654600:intron 50.0%
AGGTACCTCCTCCAGGGAAA+GGG - chr4_3:62775477-62775496 Msa0654600:three_prime_UTR 55.0%
! TAAAGCGTCCCTTTCCCTGG+AGG + chr4_3:62775488-62775507 None:intergenic 55.0%
CAAATCCATGGCTGACCTCG+TGG + chr4_3:62775607-62775626 None:intergenic 55.0%
CACGAGGTCAGCCATGGATT+TGG - chr4_3:62775605-62775624 Msa0654600:three_prime_UTR 55.0%
CGATCAATAGCTGGCAGTGG+TGG - chr4_3:62775773-62775792 Msa0654600:CDS 55.0%
! ATAGCTGGCAGTGGTGGAAC+AGG - chr4_3:62775779-62775798 Msa0654600:CDS 55.0%
GTTCTGCACAGGAACTGCAG+TGG - chr4_3:62776575-62776594 Msa0654600:intron 55.0%
AGGTACCTCCTCCAGGGAAA+GGG - chr4_3:62775477-62775496 Msa0654600:three_prime_UTR 55.0%
! TAAAGCGTCCCTTTCCCTGG+AGG + chr4_3:62775488-62775507 None:intergenic 55.0%
CAAATCCATGGCTGACCTCG+TGG + chr4_3:62775607-62775626 None:intergenic 55.0%
CACGAGGTCAGCCATGGATT+TGG - chr4_3:62775605-62775624 Msa0654600:three_prime_UTR 55.0%
CGATCAATAGCTGGCAGTGG+TGG - chr4_3:62775773-62775792 Msa0654600:CDS 55.0%
! ATAGCTGGCAGTGGTGGAAC+AGG - chr4_3:62775779-62775798 Msa0654600:CDS 55.0%
GTTCTGCACAGGAACTGCAG+TGG - chr4_3:62776575-62776594 Msa0654600:intron 55.0%
CAGGTACCTCCTCCAGGGAA+AGG - chr4_3:62775476-62775495 Msa0654600:three_prime_UTR 60.0%
CAGGTACCTCCTCCAGGGAA+AGG - chr4_3:62775476-62775495 Msa0654600:three_prime_UTR 60.0%
AGCGTCCCTTTCCCTGGAGG+AGG + chr4_3:62775485-62775504 None:intergenic 65.0%
! GCTGGCAGTGGTGGAACAGG+AGG - chr4_3:62775782-62775801 Msa0654600:CDS 65.0%
AGCGTCCCTTTCCCTGGAGG+AGG + chr4_3:62775485-62775504 None:intergenic 65.0%
! GCTGGCAGTGGTGGAACAGG+AGG - chr4_3:62775782-62775801 Msa0654600:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 62775425 62777167 62775425 ID=Msa0654600;Name=Msa0654600
chr4_3 mRNA 62775425 62777167 62775425 ID=Msa0654600-mRNA-1;Parent=Msa0654600;Name=Msa0654600-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|1|0.83|1|1|1|6|271|241
chr4_3 exon 62775425 62775810 62775425 ID=Msa0654600-mRNA-1:exon:17570;Parent=Msa0654600-mRNA-1
chr4_3 exon 62775955 62776067 62775955 ID=Msa0654600-mRNA-1:exon:17569;Parent=Msa0654600-mRNA-1
chr4_3 exon 62776185 62776280 62776185 ID=Msa0654600-mRNA-1:exon:17568;Parent=Msa0654600-mRNA-1
chr4_3 exon 62776416 62776532 62776416 ID=Msa0654600-mRNA-1:exon:17567;Parent=Msa0654600-mRNA-1
chr4_3 exon 62776760 62776858 62776760 ID=Msa0654600-mRNA-1:exon:17566;Parent=Msa0654600-mRNA-1
chr4_3 exon 62776982 62777167 62776982 ID=Msa0654600-mRNA-1:exon:17565;Parent=Msa0654600-mRNA-1
chr4_3 CDS 62776982 62777167 62776982 ID=Msa0654600-mRNA-1:cds;Parent=Msa0654600-mRNA-1
chr4_3 CDS 62776760 62776858 62776760 ID=Msa0654600-mRNA-1:cds;Parent=Msa0654600-mRNA-1
chr4_3 CDS 62776416 62776532 62776416 ID=Msa0654600-mRNA-1:cds;Parent=Msa0654600-mRNA-1
chr4_3 CDS 62776185 62776280 62776185 ID=Msa0654600-mRNA-1:cds;Parent=Msa0654600-mRNA-1
chr4_3 CDS 62775955 62776067 62775955 ID=Msa0654600-mRNA-1:cds;Parent=Msa0654600-mRNA-1
chr4_3 CDS 62775696 62775810 62775696 ID=Msa0654600-mRNA-1:cds;Parent=Msa0654600-mRNA-1
chr4_3 three_prime_UTR 62775425 62775695 62775425 ID=Msa0654600-mRNA-1:three_prime_utr;Parent=Msa0654600-mRNA-1
Gene Sequence

>Msa0654600

CGATATGCGTACACAATAGATACGAAACTAATTGAGCTTTCAAAATTGAAACAGGTACCTCCTCCAGGGAAAGGGACGCTTTATCTTAGGCCTTTGCTGATAGGAACAGGAGCTGCATTAGGCTTGGCTCCATCACCTGAGTACACATTTCTCATTTATTGCTCCCCTGTTGGAAATTACCACGAGGGAGGAAGACTAAACTTAAAAGTGGAGGATAAATTTCATCGATCAATAGCTGGCAGTGGTGGAACAGGAGGAATCAAGAGTGTTACTAATTATGCCCCGATATATACTGCAGTAACTGAAGCAAAAGCCAATGGATTTTCTGATGTCTTGTTCTTGGATTCAGCAACTGGAAACAATATTGAGGAGGCTACTGCATGCAATATATTTGTTGTGAAGGAAAATGATATCTTCACTCCGGCAATAGATGGATCTATTCTGCCTGGGGTCACACGAAAATCCATCATAGACATCGCCATTGATTTGGGTTATAAGGTCATAGAACGTTCCATATCAGTGGAGGAAATGATGAGTGCTGATGAAGTGTTCTGCACAGGAACTGCAGTGGTTGTTACCTCTGTTGCATCTGTAACATATAAGGAAACAAGAGCTGAATATAAAACAGGCGCAGAAACGTTGTCTGAAAAACTACGAGGAATACTGGTTGGAATACAAACAGGGCGTATTGAGGACAAAAAGTCATGGACAGTCCAAGTAGATTGA
Protein sequence

>Msa0654600

RYAYTIDTKLIELSKLKQVPPPGKGTLYLRPLLIGTGAALGLAPSPEYTFLIYCSPVGNYHEGGRLNLKVEDKFHRSIAGSGGTGGIKSVTNYAPIYTAVTEAKANGFSDVLFLDSATGNNIEEATACNIFVVKENDIFTPAIDGSILPGVTRKSIIDIAIDLGYKVIERSISVEEMMSADEVFCTGTAVVVTSVASVTYKETRAEYKTGAETLSEKLRGILVGIQTGRIEDKKSWTVQVD*