AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1381900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1381900 MtrunA17_Chr4g0049241 95.197 229 11 0 13 241 176 404 1.17e-148 420
Msa1381900 MtrunA17_Chr3g0135321 56.596 235 98 2 9 241 155 387 7.33e-90 270
Msa1381900 MtrunA17_Chr4g0049231 53.744 227 101 2 17 241 133 357 8.37e-83 251
Msa1381900 MtrunA17_Chr5g0400811 53.913 230 102 2 13 240 180 407 8.56e-81 248
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1381900 AT1G50110.2 61.739 230 85 2 13 241 90 317 1.17e-100 296
Msa1381900 AT1G50110.1 61.739 230 85 2 13 241 125 352 4.24e-100 295
Msa1381900 AT1G50090.1 59.130 230 91 2 13 241 128 355 2.56e-95 284
Msa1381900 AT1G10070.3 57.021 235 97 2 9 241 86 318 3.50e-95 281
Msa1381900 AT1G10070.1 57.021 235 97 2 9 241 156 388 2.29e-94 282
Msa1381900 AT1G10070.2 57.021 235 97 2 9 241 156 388 2.29e-94 282
Msa1381900 AT1G10060.3 58.261 230 92 2 13 240 90 317 7.51e-91 271
Msa1381900 AT1G10060.2 58.261 230 92 2 13 240 156 383 6.05e-90 271
Msa1381900 AT1G10060.5 58.261 230 92 2 13 240 252 479 8.49e-89 271
Msa1381900 AT3G19710.1 53.846 234 105 2 9 241 120 351 3.46e-88 265
Msa1381900 AT3G49680.2 56.277 231 96 3 13 240 182 410 3.31e-85 259
Msa1381900 AT3G49680.1 56.277 231 96 3 13 240 184 412 3.90e-85 259
Msa1381900 AT5G65780.1 55.411 231 98 3 13 240 186 414 1.16e-83 255
Msa1381900 AT1G10060.7 58.974 195 76 2 13 205 156 348 8.20e-74 229
Msa1381900 AT1G10060.4 59.487 195 75 2 13 205 252 444 1.01e-72 229
Msa1381900 AT1G10060.1 55.944 143 59 2 13 153 156 296 7.66e-48 160
Msa1381900 AT1G10060.6 55.944 143 59 2 13 153 252 392 7.51e-47 160

Find 71 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCCATTGATTTGGGTTATA+AGG 0.149101 tig0020460:-33321 Msa1381900:intron
TAAGTTCAGTTTATTATAAA+TGG 0.157123 tig0020460:+32653 None:intergenic
GTTTATTATAAATGGAAATT+TGG 0.163762 tig0020460:+32661 None:intergenic
GTCCAAGTAGATTGAGAAAT+TGG 0.258521 tig0020460:-32825 Msa1381900:exon
CATAGACATCGCCATTGATT+TGG 0.259021 tig0020460:-33330 Msa1381900:CDS
CTAATCATCAATAGTATGTT+TGG 0.287341 tig0020460:-32759 Msa1381900:three_prime_UTR
TGTTGCATCTGTAACATATA+AGG 0.310394 tig0020460:-33099 Msa1381900:CDS
CATCATTTCCTCCACTGATA+TGG 0.317405 tig0020460:+33169 None:intergenic
GGAAAGGGACGCTTTATCTT+AGG 0.336223 tig0020460:-34215 Msa1381900:CDS
CAAACATACTATTGATGATT+AGG 0.337825 tig0020460:+32760 None:intergenic
ATCTTCACTCCGGCAATAGA+TGG 0.342656 tig0020460:-33386 Msa1381900:CDS
TATCTTAGGCCTTTGCTGAT+AGG 0.349392 tig0020460:-34201 Msa1381900:CDS
TTGTTCTTGGATTCAGCAAC+TGG 0.355547 tig0020460:-33599 Msa1381900:CDS
ATAGACATCGCCATTGATTT+GGG 0.368721 tig0020460:-33329 Msa1381900:CDS
TGAGCTTTCAAAATTGAAAC+AGG 0.392634 tig0020460:-34250 Msa1381900:CDS
ACCTCGTGGTAATTTCCAAC+AGG 0.408370 tig0020460:+34117 None:intergenic
AGGCCTTTGCTGATAGGAAC+AGG 0.426821 tig0020460:-34195 Msa1381900:CDS
TGCCAATTTCTCAATCTACT+TGG 0.427171 tig0020460:+32823 None:intergenic
ATACTGGTTGGAATACAAAC+AGG 0.434484 tig0020460:-32876 Msa1381900:CDS
ATAGCTGGCAGTGGTGGAAC+AGG 0.460699 tig0020460:-33928 Msa1381900:CDS
AGAATAGATCCATCTATTGC+CGG 0.463647 tig0020460:+33377 None:intergenic
CTCATTTATTGCTCCCCTGT+TGG 0.463936 tig0020460:-34132 Msa1381900:CDS
TTGAAACAGGTACCTCCTCC+AGG 0.466899 tig0020460:-34237 Msa1381900:CDS
TGCAGAGCTGAATATAAAAC+AGG 0.474986 tig0020460:-32930 Msa1381900:intron
ATCAATGGCGATGTCTATGA+TGG 0.477143 tig0020460:+33334 None:intergenic
GATTTCAGTTGCAAACATAC+AGG 0.481875 tig0020460:-32737 Msa1381900:three_prime_UTR
AGATAAAGCGTCCCTTTCCC+TGG 0.488127 tig0020460:+34219 None:intergenic
ATAGATGGATCTATTCTGCC+TGG 0.500850 tig0020460:-33371 Msa1381900:CDS
TACCTTATAACCCAAATCAA+TGG 0.507754 tig0020460:+33319 None:intergenic
TATAAATGGAAATTTGGACT+TGG 0.509183 tig0020460:+32667 None:intergenic
AGTTGCAAACATACAGGTAG+AGG 0.514094 tig0020460:-32731 Msa1381900:three_prime_UTR
ATAGGAACAGGAGCTGCATT+AGG 0.514522 tig0020460:-34183 Msa1381900:CDS
AACAGGAGCTGCATTAGGCT+TGG 0.520575 tig0020460:-34178 Msa1381900:CDS
TAGATGGATCTATTCTGCCT+GGG 0.527089 tig0020460:-33370 Msa1381900:CDS
CGATCAATAGCTGGCAGTGG+TGG 0.529542 tig0020460:-33934 Msa1381900:CDS
AGGTACCTCCTCCAGGGAAA+GGG 0.530899 tig0020460:-34230 Msa1381900:CDS
AAACTACGAGGAATACTGGT+TGG 0.534155 tig0020460:-32888 Msa1381900:CDS
CAGGTACCTCCTCCAGGGAA+AGG 0.544439 tig0020460:-34231 Msa1381900:CDS
AAATTTCATCGATCAATAGC+TGG 0.549508 tig0020460:-33943 Msa1381900:CDS
AGGAAGACTAAACTTAAAAG+TGG 0.553988 tig0020460:-33971 Msa1381900:CDS
AGCGTCCCTTTCCCTGGAGG+AGG 0.558749 tig0020460:+34225 None:intergenic
CTGTAACATATAAGGAAACA+AGG 0.572784 tig0020460:-33091 Msa1381900:intron
CAAGACATCAGAAAATCCAT+TGG 0.572958 tig0020460:+33619 None:intergenic
TAAATGAGAAATGTGTACTC+AGG 0.581598 tig0020460:+34147 None:intergenic
GTAACTGAAGCAAAAGCCAA+TGG 0.582493 tig0020460:-33635 Msa1381900:CDS
ATGCAATATATTTGTTGTGA+AGG 0.592887 tig0020460:-33552 Msa1381900:intron
GGAAAATGATATCTTCACTC+CGG 0.596052 tig0020460:-33396 Msa1381900:CDS
AATACAAACAGGGCGTATTG+AGG 0.599236 tig0020460:-32865 Msa1381900:CDS
AGCAACTGGAAACAATATTG+AGG 0.599305 tig0020460:-33585 Msa1381900:CDS
GCTGGCAGTGGTGGAACAGG+AGG 0.602903 tig0020460:-33925 Msa1381900:CDS
GTGTGTGAATCTGCTTTGTG+TGG 0.604347 tig0020460:-32605 Msa1381900:three_prime_UTR
CATCGATCAATAGCTGGCAG+TGG 0.604641 tig0020460:-33937 Msa1381900:CDS
CATAGAACGTTCCATATCAG+TGG 0.605813 tig0020460:-33180 Msa1381900:CDS
AGAAATGTGTACTCAGGTGA+TGG 0.612357 tig0020460:+34153 None:intergenic
GCTGATGAAGTGTTCTGCAC+AGG 0.613446 tig0020460:-33143 Msa1381900:CDS
GCTCCTGTTCCTATCAGCAA+AGG 0.618333 tig0020460:+34192 None:intergenic
AAGACTAAACTTAAAAGTGG+AGG 0.620669 tig0020460:-33968 Msa1381900:CDS
GAGTGTTACTAATTATGCCC+CGG 0.621088 tig0020460:-33896 Msa1381900:intron
CCTCGTGGTAATTTCCAACA+GGG 0.633692 tig0020460:+34118 None:intergenic
TGAAACAGGTACCTCCTCCA+GGG 0.635669 tig0020460:-34236 Msa1381900:CDS
AGAACGTTCCATATCAGTGG+AGG 0.645297 tig0020460:-33177 Msa1381900:CDS
AACTGGAAACAATATTGAGG+AGG 0.649999 tig0020460:-33582 Msa1381900:CDS
TTCCAAGCTACTGGCTACAA+CGG 0.650241 tig0020460:+32552 None:intergenic
AGATGGATCTATTCTGCCTG+GGG 0.660994 tig0020460:-33369 Msa1381900:CDS
ACAAGTATTATCATTTAACA+AGG 0.662636 tig0020460:+32797 None:intergenic
TAAAGCGTCCCTTTCCCTGG+AGG 0.677703 tig0020460:+34222 None:intergenic
ATATGTTACAGATGCAACAG+AGG 0.681902 tig0020460:+33103 None:intergenic
TACTGGTTGGAATACAAACA+GGG 0.699480 tig0020460:-32875 Msa1381900:CDS
CTCGTGGTAATTTCCAACAG+GGG 0.700556 tig0020460:+34119 None:intergenic
CCCTGTTGGAAATTACCACG+AGG 0.728651 tig0020460:-34118 Msa1381900:intron
GTTCTGCACAGGAACTGCAG+TGG 0.751564 tig0020460:-33132 Msa1381900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGGAAAATATATAATAATT+TGG + tig0020460:33035-33054 None:intergenic 10.0%
!!! AAATTATTATATATTTTCCT+TGG - tig0020460:33034-33053 Msa1381900:intron 10.0%
!! AAGGAAAATATATAATAATT+TGG + tig0020460:33035-33054 None:intergenic 10.0%
!!! AAATTATTATATATTTTCCT+TGG - tig0020460:33034-33053 Msa1381900:intron 10.0%
!!! CTTATATTGTTTTGTTAATA+AGG - tig0020460:32770-32789 Msa1381900:three_prime_UTR 15.0%
!! TGTAAATTTGTAGAAAATAA+TGG + tig0020460:33575-33594 None:intergenic 15.0%
!! TATAGATGATTAATGATTTA+TGG - tig0020460:33596-33615 Msa1381900:CDS 15.0%
!! AAAAATTGACTAAAATGAAA+GGG + tig0020460:33834-33853 None:intergenic 15.0%
!! AAAAAATTGACTAAAATGAA+AGG + tig0020460:33835-33854 None:intergenic 15.0%
!!! TTTTAGTCAATTTTTTTTGT+TGG - tig0020460:33839-33858 Msa1381900:intron 15.0%
!! GTTTATTATAAATGGAAATT+TGG + tig0020460:34186-34205 None:intergenic 15.0%
!! TAAGTTCAGTTTATTATAAA+TGG + tig0020460:34194-34213 None:intergenic 15.0%
!!! CTTATATTGTTTTGTTAATA+AGG - tig0020460:32770-32789 Msa1381900:three_prime_UTR 15.0%
!! TGTAAATTTGTAGAAAATAA+TGG + tig0020460:33575-33594 None:intergenic 15.0%
!! TATAGATGATTAATGATTTA+TGG - tig0020460:33596-33615 Msa1381900:CDS 15.0%
!! AAAAATTGACTAAAATGAAA+GGG + tig0020460:33834-33853 None:intergenic 15.0%
!! AAAAAATTGACTAAAATGAA+AGG + tig0020460:33835-33854 None:intergenic 15.0%
!!! TTTTAGTCAATTTTTTTTGT+TGG - tig0020460:33839-33858 Msa1381900:intron 15.0%
!! GTTTATTATAAATGGAAATT+TGG + tig0020460:34186-34205 None:intergenic 15.0%
!! TAAGTTCAGTTTATTATAAA+TGG + tig0020460:34194-34213 None:intergenic 15.0%
!! AAAAAAAAAAAATAACAGGG+AGG - tig0020460:32853-32872 Msa1381900:CDS 20.0%
!! ACAAGTATTATCATTTAACA+AGG + tig0020460:34050-34069 None:intergenic 20.0%
!! AAAAAAAAAAAATAACAGGG+AGG - tig0020460:32853-32872 Msa1381900:CDS 20.0%
!! ACAAGTATTATCATTTAACA+AGG + tig0020460:34050-34069 None:intergenic 20.0%
! TATAAGTAAATCCAAATCCA+TGG + tig0020460:32756-32775 None:intergenic 25.0%
!!! ATGCAATATATTTGTTGTGA+AGG - tig0020460:33292-33311 Msa1381900:intron 25.0%
! GTTTCGGATATAGTAAAATA+TGG + tig0020460:33386-33405 None:intergenic 25.0%
! ACTATATCCGAAACAAAAAT+TGG - tig0020460:33392-33411 Msa1381900:CDS 25.0%
!!! CATTAGACCAATTTTTGTTT+CGG + tig0020460:33402-33421 None:intergenic 25.0%
!! GTTTTGCATTAATCTTGTTA+AGG - tig0020460:33427-33446 Msa1381900:intron 25.0%
! AGAAAATAATGGCAAACATT+GGG + tig0020460:33564-33583 None:intergenic 25.0%
! TAGAAAATAATGGCAAACAT+TGG + tig0020460:33565-33584 None:intergenic 25.0%
! TTTGTAACAAAACTATGTTC+AGG - tig0020460:33640-33659 Msa1381900:CDS 25.0%
! TTGTGTTTAGCATTATCATT+AGG + tig0020460:33879-33898 None:intergenic 25.0%
!! TAATGATAATGCTAAACACA+AGG - tig0020460:33878-33897 Msa1381900:intron 25.0%
!! CAAACATACTATTGATGATT+AGG + tig0020460:34087-34106 None:intergenic 25.0%
! CTAATCATCAATAGTATGTT+TGG - tig0020460:34085-34104 Msa1381900:intron 25.0%
! TATAAATGGAAATTTGGACT+TGG + tig0020460:34180-34199 None:intergenic 25.0%
! TATAAGTAAATCCAAATCCA+TGG + tig0020460:32756-32775 None:intergenic 25.0%
!!! ATGCAATATATTTGTTGTGA+AGG - tig0020460:33292-33311 Msa1381900:intron 25.0%
! GTTTCGGATATAGTAAAATA+TGG + tig0020460:33386-33405 None:intergenic 25.0%
! ACTATATCCGAAACAAAAAT+TGG - tig0020460:33392-33411 Msa1381900:CDS 25.0%
!!! CATTAGACCAATTTTTGTTT+CGG + tig0020460:33402-33421 None:intergenic 25.0%
!! GTTTTGCATTAATCTTGTTA+AGG - tig0020460:33427-33446 Msa1381900:intron 25.0%
! AGAAAATAATGGCAAACATT+GGG + tig0020460:33564-33583 None:intergenic 25.0%
! TAGAAAATAATGGCAAACAT+TGG + tig0020460:33565-33584 None:intergenic 25.0%
! TTTGTAACAAAACTATGTTC+AGG - tig0020460:33640-33659 Msa1381900:CDS 25.0%
! TTGTGTTTAGCATTATCATT+AGG + tig0020460:33879-33898 None:intergenic 25.0%
!! TAATGATAATGCTAAACACA+AGG - tig0020460:33878-33897 Msa1381900:intron 25.0%
!! CAAACATACTATTGATGATT+AGG + tig0020460:34087-34106 None:intergenic 25.0%
! CTAATCATCAATAGTATGTT+TGG - tig0020460:34085-34104 Msa1381900:intron 25.0%
! TATAAATGGAAATTTGGACT+TGG + tig0020460:34180-34199 None:intergenic 25.0%
TGAGCTTTCAAAATTGAAAC+AGG - tig0020460:32594-32613 Msa1381900:three_prime_UTR 30.0%
TAAATGAGAAATGTGTACTC+AGG + tig0020460:32700-32719 None:intergenic 30.0%
AGGAAGACTAAACTTAAAAG+TGG - tig0020460:32873-32892 Msa1381900:CDS 30.0%
AAGACTAAACTTAAAAGTGG+AGG - tig0020460:32876-32895 Msa1381900:CDS 30.0%
AAATTTCATCGATCAATAGC+TGG - tig0020460:32901-32920 Msa1381900:CDS 30.0%
TTATATACATCACCGTTTGA+TGG - tig0020460:32999-33018 Msa1381900:intron 30.0%
GGAGAAAAAAAACCATCAAA+CGG + tig0020460:33014-33033 None:intergenic 30.0%
TTGAGAAGAAATCACATCAA+AGG - tig0020460:33083-33102 Msa1381900:intron 30.0%
! ACTGATGCATATTGTTACTT+AGG - tig0020460:33106-33125 Msa1381900:CDS 30.0%
CTCTTCATTATGCATATTTC+TGG - tig0020460:33166-33185 Msa1381900:CDS 30.0%
!! ATTTTCTGATGTCTTGTTCT+TGG - tig0020460:33232-33251 Msa1381900:intron 30.0%
TACCTTATAACCCAAATCAA+TGG + tig0020460:33528-33547 None:intergenic 30.0%
TGTTGCATCTGTAACATATA+AGG - tig0020460:33745-33764 Msa1381900:intron 30.0%
CTGTAACATATAAGGAAACA+AGG - tig0020460:33753-33772 Msa1381900:intron 30.0%
TGAGCTTTCAAAATTGAAAC+AGG - tig0020460:32594-32613 Msa1381900:three_prime_UTR 30.0%
TAAATGAGAAATGTGTACTC+AGG + tig0020460:32700-32719 None:intergenic 30.0%
AGGAAGACTAAACTTAAAAG+TGG - tig0020460:32873-32892 Msa1381900:CDS 30.0%
AAGACTAAACTTAAAAGTGG+AGG - tig0020460:32876-32895 Msa1381900:CDS 30.0%
AAATTTCATCGATCAATAGC+TGG - tig0020460:32901-32920 Msa1381900:CDS 30.0%
TTATATACATCACCGTTTGA+TGG - tig0020460:32999-33018 Msa1381900:intron 30.0%
GGAGAAAAAAAACCATCAAA+CGG + tig0020460:33014-33033 None:intergenic 30.0%
TTGAGAAGAAATCACATCAA+AGG - tig0020460:33083-33102 Msa1381900:intron 30.0%
! ACTGATGCATATTGTTACTT+AGG - tig0020460:33106-33125 Msa1381900:CDS 30.0%
CTCTTCATTATGCATATTTC+TGG - tig0020460:33166-33185 Msa1381900:CDS 30.0%
!! ATTTTCTGATGTCTTGTTCT+TGG - tig0020460:33232-33251 Msa1381900:intron 30.0%
TACCTTATAACCCAAATCAA+TGG + tig0020460:33528-33547 None:intergenic 30.0%
TGTTGCATCTGTAACATATA+AGG - tig0020460:33745-33764 Msa1381900:intron 30.0%
CTGTAACATATAAGGAAACA+AGG - tig0020460:33753-33772 Msa1381900:intron 30.0%
CAAGACATCAGAAAATCCAT+TGG + tig0020460:33228-33247 None:intergenic 35.0%
AGCAACTGGAAACAATATTG+AGG - tig0020460:33259-33278 Msa1381900:intron 35.0%
AACTGGAAACAATATTGAGG+AGG - tig0020460:33262-33281 Msa1381900:intron 35.0%
GGAAAATGATATCTTCACTC+CGG - tig0020460:33448-33467 Msa1381900:intron 35.0%
AGAATAGATCCATCTATTGC+CGG + tig0020460:33470-33489 None:intergenic 35.0%
!! ATAGACATCGCCATTGATTT+GGG - tig0020460:33515-33534 Msa1381900:intron 35.0%
AATAATGGCAAACATTGGGA+GGG + tig0020460:33560-33579 None:intergenic 35.0%
AAATAATGGCAAACATTGGG+AGG + tig0020460:33561-33580 None:intergenic 35.0%
ATATGTTACAGATGCAACAG+AGG + tig0020460:33744-33763 None:intergenic 35.0%
TGCAGAGCTGAATATAAAAC+AGG - tig0020460:33914-33933 Msa1381900:CDS 35.0%
TGAAAAACTACGAGGAATAC+TGG - tig0020460:33952-33971 Msa1381900:CDS 35.0%
! ATACTGGTTGGAATACAAAC+AGG - tig0020460:33968-33987 Msa1381900:CDS 35.0%
! TACTGGTTGGAATACAAACA+GGG - tig0020460:33969-33988 Msa1381900:CDS 35.0%
GTATTGAGGACAAAAAGTCA+TGG - tig0020460:33993-34012 Msa1381900:intron 35.0%
GTCCAAGTAGATTGAGAAAT+TGG - tig0020460:34019-34038 Msa1381900:intron 35.0%
TGCCAATTTCTCAATCTACT+TGG + tig0020460:34024-34043 None:intergenic 35.0%
GATTTCAGTTGCAAACATAC+AGG - tig0020460:34107-34126 Msa1381900:intron 35.0%
CAAGACATCAGAAAATCCAT+TGG + tig0020460:33228-33247 None:intergenic 35.0%
AGCAACTGGAAACAATATTG+AGG - tig0020460:33259-33278 Msa1381900:intron 35.0%
AACTGGAAACAATATTGAGG+AGG - tig0020460:33262-33281 Msa1381900:intron 35.0%
GGAAAATGATATCTTCACTC+CGG - tig0020460:33448-33467 Msa1381900:intron 35.0%
AGAATAGATCCATCTATTGC+CGG + tig0020460:33470-33489 None:intergenic 35.0%
!! ATAGACATCGCCATTGATTT+GGG - tig0020460:33515-33534 Msa1381900:intron 35.0%
AATAATGGCAAACATTGGGA+GGG + tig0020460:33560-33579 None:intergenic 35.0%
AAATAATGGCAAACATTGGG+AGG + tig0020460:33561-33580 None:intergenic 35.0%
ATATGTTACAGATGCAACAG+AGG + tig0020460:33744-33763 None:intergenic 35.0%
TGCAGAGCTGAATATAAAAC+AGG - tig0020460:33914-33933 Msa1381900:CDS 35.0%
TGAAAAACTACGAGGAATAC+TGG - tig0020460:33952-33971 Msa1381900:CDS 35.0%
! ATACTGGTTGGAATACAAAC+AGG - tig0020460:33968-33987 Msa1381900:CDS 35.0%
! TACTGGTTGGAATACAAACA+GGG - tig0020460:33969-33988 Msa1381900:CDS 35.0%
GTATTGAGGACAAAAAGTCA+TGG - tig0020460:33993-34012 Msa1381900:intron 35.0%
GTCCAAGTAGATTGAGAAAT+TGG - tig0020460:34019-34038 Msa1381900:intron 35.0%
TGCCAATTTCTCAATCTACT+TGG + tig0020460:34024-34043 None:intergenic 35.0%
GATTTCAGTTGCAAACATAC+AGG - tig0020460:34107-34126 Msa1381900:intron 35.0%
TATCTTAGGCCTTTGCTGAT+AGG - tig0020460:32643-32662 Msa1381900:three_prime_UTR 40.0%
AGAAATGTGTACTCAGGTGA+TGG + tig0020460:32694-32713 None:intergenic 40.0%
! GAGTGTTACTAATTATGCCC+CGG - tig0020460:32948-32967 Msa1381900:intron 40.0%
ATCACTATTGCCATGCTTAC+CGG + tig0020460:32970-32989 None:intergenic 40.0%
! CTTTTCACAAGTCACAACCA+AGG + tig0020460:33054-33073 None:intergenic 40.0%
GTAACTGAAGCAAAAGCCAA+TGG - tig0020460:33209-33228 Msa1381900:intron 40.0%
TTGTTCTTGGATTCAGCAAC+TGG - tig0020460:33245-33264 Msa1381900:intron 40.0%
ATAGATGGATCTATTCTGCC+TGG - tig0020460:33473-33492 Msa1381900:intron 40.0%
TAGATGGATCTATTCTGCCT+GGG - tig0020460:33474-33493 Msa1381900:intron 40.0%
ATCAATGGCGATGTCTATGA+TGG + tig0020460:33513-33532 None:intergenic 40.0%
! CATAGACATCGCCATTGATT+TGG - tig0020460:33514-33533 Msa1381900:intron 40.0%
!! CGCCATTGATTTGGGTTATA+AGG - tig0020460:33523-33542 Msa1381900:intron 40.0%
CATAGAACGTTCCATATCAG+TGG - tig0020460:33664-33683 Msa1381900:intron 40.0%
CATCATTTCCTCCACTGATA+TGG + tig0020460:33678-33697 None:intergenic 40.0%
ACGTTGTCTGAAAAACTACG+AGG - tig0020460:33944-33963 Msa1381900:CDS 40.0%
AAACTACGAGGAATACTGGT+TGG - tig0020460:33956-33975 Msa1381900:CDS 40.0%
AATACAAACAGGGCGTATTG+AGG - tig0020460:33979-33998 Msa1381900:intron 40.0%
AGTTGCAAACATACAGGTAG+AGG - tig0020460:34113-34132 Msa1381900:intron 40.0%
TATCTTAGGCCTTTGCTGAT+AGG - tig0020460:32643-32662 Msa1381900:three_prime_UTR 40.0%
AGAAATGTGTACTCAGGTGA+TGG + tig0020460:32694-32713 None:intergenic 40.0%
! GAGTGTTACTAATTATGCCC+CGG - tig0020460:32948-32967 Msa1381900:intron 40.0%
ATCACTATTGCCATGCTTAC+CGG + tig0020460:32970-32989 None:intergenic 40.0%
! CTTTTCACAAGTCACAACCA+AGG + tig0020460:33054-33073 None:intergenic 40.0%
GTAACTGAAGCAAAAGCCAA+TGG - tig0020460:33209-33228 Msa1381900:intron 40.0%
TTGTTCTTGGATTCAGCAAC+TGG - tig0020460:33245-33264 Msa1381900:intron 40.0%
ATAGATGGATCTATTCTGCC+TGG - tig0020460:33473-33492 Msa1381900:intron 40.0%
TAGATGGATCTATTCTGCCT+GGG - tig0020460:33474-33493 Msa1381900:intron 40.0%
ATCAATGGCGATGTCTATGA+TGG + tig0020460:33513-33532 None:intergenic 40.0%
! CATAGACATCGCCATTGATT+TGG - tig0020460:33514-33533 Msa1381900:intron 40.0%
!! CGCCATTGATTTGGGTTATA+AGG - tig0020460:33523-33542 Msa1381900:intron 40.0%
CATAGAACGTTCCATATCAG+TGG - tig0020460:33664-33683 Msa1381900:intron 40.0%
CATCATTTCCTCCACTGATA+TGG + tig0020460:33678-33697 None:intergenic 40.0%
ACGTTGTCTGAAAAACTACG+AGG - tig0020460:33944-33963 Msa1381900:CDS 40.0%
AAACTACGAGGAATACTGGT+TGG - tig0020460:33956-33975 Msa1381900:CDS 40.0%
AATACAAACAGGGCGTATTG+AGG - tig0020460:33979-33998 Msa1381900:intron 40.0%
AGTTGCAAACATACAGGTAG+AGG - tig0020460:34113-34132 Msa1381900:intron 40.0%
GGAAAGGGACGCTTTATCTT+AGG - tig0020460:32629-32648 Msa1381900:three_prime_UTR 45.0%
ATAGGAACAGGAGCTGCATT+AGG - tig0020460:32661-32680 Msa1381900:three_prime_UTR 45.0%
CTCATTTATTGCTCCCCTGT+TGG - tig0020460:32712-32731 Msa1381900:three_prime_UTR 45.0%
CTCGTGGTAATTTCCAACAG+GGG + tig0020460:32728-32747 None:intergenic 45.0%
CCTCGTGGTAATTTCCAACA+GGG + tig0020460:32729-32748 None:intergenic 45.0%
ACCTCGTGGTAATTTCCAAC+AGG + tig0020460:32730-32749 None:intergenic 45.0%
TAATTATGCCCCGGTAAGCA+TGG - tig0020460:32957-32976 Msa1381900:intron 45.0%
TCACTATTGCCATGCTTACC+GGG + tig0020460:32969-32988 None:intergenic 45.0%
ATCTTCACTCCGGCAATAGA+TGG - tig0020460:33458-33477 Msa1381900:intron 45.0%
AGATGGATCTATTCTGCCTG+GGG - tig0020460:33475-33494 Msa1381900:intron 45.0%
AGAACGTTCCATATCAGTGG+AGG - tig0020460:33667-33686 Msa1381900:intron 45.0%
GTGTGTGAATCTGCTTTGTG+TGG - tig0020460:34239-34258 Msa1381900:CDS 45.0%
GGAAAGGGACGCTTTATCTT+AGG - tig0020460:32629-32648 Msa1381900:three_prime_UTR 45.0%
ATAGGAACAGGAGCTGCATT+AGG - tig0020460:32661-32680 Msa1381900:three_prime_UTR 45.0%
CTCATTTATTGCTCCCCTGT+TGG - tig0020460:32712-32731 Msa1381900:three_prime_UTR 45.0%
CTCGTGGTAATTTCCAACAG+GGG + tig0020460:32728-32747 None:intergenic 45.0%
CCTCGTGGTAATTTCCAACA+GGG + tig0020460:32729-32748 None:intergenic 45.0%
ACCTCGTGGTAATTTCCAAC+AGG + tig0020460:32730-32749 None:intergenic 45.0%
TAATTATGCCCCGGTAAGCA+TGG - tig0020460:32957-32976 Msa1381900:intron 45.0%
TCACTATTGCCATGCTTACC+GGG + tig0020460:32969-32988 None:intergenic 45.0%
ATCTTCACTCCGGCAATAGA+TGG - tig0020460:33458-33477 Msa1381900:intron 45.0%
AGATGGATCTATTCTGCCTG+GGG - tig0020460:33475-33494 Msa1381900:intron 45.0%
AGAACGTTCCATATCAGTGG+AGG - tig0020460:33667-33686 Msa1381900:intron 45.0%
GTGTGTGAATCTGCTTTGTG+TGG - tig0020460:34239-34258 Msa1381900:CDS 45.0%
!!! TTTTTTTAAAAAAAAAAAGA+AGG + tig0020460:32839-32858 None:intergenic 5.0%
!! TTTAAAAAAAAAAAAATAAC+AGG - tig0020460:32849-32868 Msa1381900:CDS 5.0%
!! TTAAAAAAAAAAAAATAACA+GGG - tig0020460:32850-32869 Msa1381900:CDS 5.0%
!!! TTTTTTTAAAAAAAAAAAGA+AGG + tig0020460:32839-32858 None:intergenic 5.0%
!! TTTAAAAAAAAAAAAATAAC+AGG - tig0020460:32849-32868 Msa1381900:CDS 5.0%
!! TTAAAAAAAAAAAAATAACA+GGG - tig0020460:32850-32869 Msa1381900:CDS 5.0%
TTGAAACAGGTACCTCCTCC+AGG - tig0020460:32607-32626 Msa1381900:three_prime_UTR 50.0%
TGAAACAGGTACCTCCTCCA+GGG - tig0020460:32608-32627 Msa1381900:three_prime_UTR 50.0%
! AGATAAAGCGTCCCTTTCCC+TGG + tig0020460:32628-32647 None:intergenic 50.0%
AGGCCTTTGCTGATAGGAAC+AGG - tig0020460:32649-32668 Msa1381900:three_prime_UTR 50.0%
GCTCCTGTTCCTATCAGCAA+AGG + tig0020460:32655-32674 None:intergenic 50.0%
AACAGGAGCTGCATTAGGCT+TGG - tig0020460:32666-32685 Msa1381900:three_prime_UTR 50.0%
CCCTGTTGGAAATTACCACG+AGG - tig0020460:32726-32745 Msa1381900:three_prime_UTR 50.0%
AATTACCACGAGGTCAGCCA+TGG - tig0020460:32736-32755 Msa1381900:three_prime_UTR 50.0%
CATCGATCAATAGCTGGCAG+TGG - tig0020460:32907-32926 Msa1381900:CDS 50.0%
CACTATTGCCATGCTTACCG+GGG + tig0020460:32968-32987 None:intergenic 50.0%
! ATGGATTTTCGTGTGACCCC+AGG + tig0020460:33494-33513 None:intergenic 50.0%
! GCTGATGAAGTGTTCTGCAC+AGG - tig0020460:33701-33720 Msa1381900:intron 50.0%
TTGAAACAGGTACCTCCTCC+AGG - tig0020460:32607-32626 Msa1381900:three_prime_UTR 50.0%
TGAAACAGGTACCTCCTCCA+GGG - tig0020460:32608-32627 Msa1381900:three_prime_UTR 50.0%
! AGATAAAGCGTCCCTTTCCC+TGG + tig0020460:32628-32647 None:intergenic 50.0%
AGGCCTTTGCTGATAGGAAC+AGG - tig0020460:32649-32668 Msa1381900:three_prime_UTR 50.0%
GCTCCTGTTCCTATCAGCAA+AGG + tig0020460:32655-32674 None:intergenic 50.0%
AACAGGAGCTGCATTAGGCT+TGG - tig0020460:32666-32685 Msa1381900:three_prime_UTR 50.0%
CCCTGTTGGAAATTACCACG+AGG - tig0020460:32726-32745 Msa1381900:three_prime_UTR 50.0%
AATTACCACGAGGTCAGCCA+TGG - tig0020460:32736-32755 Msa1381900:three_prime_UTR 50.0%
CATCGATCAATAGCTGGCAG+TGG - tig0020460:32907-32926 Msa1381900:CDS 50.0%
CACTATTGCCATGCTTACCG+GGG + tig0020460:32968-32987 None:intergenic 50.0%
! ATGGATTTTCGTGTGACCCC+AGG + tig0020460:33494-33513 None:intergenic 50.0%
! GCTGATGAAGTGTTCTGCAC+AGG - tig0020460:33701-33720 Msa1381900:intron 50.0%
AGGTACCTCCTCCAGGGAAA+GGG - tig0020460:32614-32633 Msa1381900:three_prime_UTR 55.0%
! TAAAGCGTCCCTTTCCCTGG+AGG + tig0020460:32625-32644 None:intergenic 55.0%
CAAATCCATGGCTGACCTCG+TGG + tig0020460:32744-32763 None:intergenic 55.0%
CACGAGGTCAGCCATGGATT+TGG - tig0020460:32742-32761 Msa1381900:three_prime_UTR 55.0%
CGATCAATAGCTGGCAGTGG+TGG - tig0020460:32910-32929 Msa1381900:CDS 55.0%
! ATAGCTGGCAGTGGTGGAAC+AGG - tig0020460:32916-32935 Msa1381900:CDS 55.0%
GTTCTGCACAGGAACTGCAG+TGG - tig0020460:33712-33731 Msa1381900:intron 55.0%
AGGTACCTCCTCCAGGGAAA+GGG - tig0020460:32614-32633 Msa1381900:three_prime_UTR 55.0%
! TAAAGCGTCCCTTTCCCTGG+AGG + tig0020460:32625-32644 None:intergenic 55.0%
CAAATCCATGGCTGACCTCG+TGG + tig0020460:32744-32763 None:intergenic 55.0%
CACGAGGTCAGCCATGGATT+TGG - tig0020460:32742-32761 Msa1381900:three_prime_UTR 55.0%
CGATCAATAGCTGGCAGTGG+TGG - tig0020460:32910-32929 Msa1381900:CDS 55.0%
! ATAGCTGGCAGTGGTGGAAC+AGG - tig0020460:32916-32935 Msa1381900:CDS 55.0%
GTTCTGCACAGGAACTGCAG+TGG - tig0020460:33712-33731 Msa1381900:intron 55.0%
CAGGTACCTCCTCCAGGGAA+AGG - tig0020460:32613-32632 Msa1381900:three_prime_UTR 60.0%
CAGGTACCTCCTCCAGGGAA+AGG - tig0020460:32613-32632 Msa1381900:three_prime_UTR 60.0%
AGCGTCCCTTTCCCTGGAGG+AGG + tig0020460:32622-32641 None:intergenic 65.0%
! GCTGGCAGTGGTGGAACAGG+AGG - tig0020460:32919-32938 Msa1381900:CDS 65.0%
AGCGTCCCTTTCCCTGGAGG+AGG + tig0020460:32622-32641 None:intergenic 65.0%
! GCTGGCAGTGGTGGAACAGG+AGG - tig0020460:32919-32938 Msa1381900:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0020460 gene 32562 34304 32562 ID=Msa1381900;Name=Msa1381900
tig0020460 mRNA 32562 34304 32562 ID=Msa1381900-mRNA-1;Parent=Msa1381900;Name=Msa1381900-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|1|0.83|1|1|1|6|271|241
tig0020460 exon 32562 32947 32562 ID=Msa1381900-mRNA-1:exon:7853;Parent=Msa1381900-mRNA-1
tig0020460 exon 33092 33204 33092 ID=Msa1381900-mRNA-1:exon:7852;Parent=Msa1381900-mRNA-1
tig0020460 exon 33322 33417 33322 ID=Msa1381900-mRNA-1:exon:7851;Parent=Msa1381900-mRNA-1
tig0020460 exon 33553 33669 33553 ID=Msa1381900-mRNA-1:exon:7850;Parent=Msa1381900-mRNA-1
tig0020460 exon 33897 33995 33897 ID=Msa1381900-mRNA-1:exon:7849;Parent=Msa1381900-mRNA-1
tig0020460 exon 34119 34304 34119 ID=Msa1381900-mRNA-1:exon:7848;Parent=Msa1381900-mRNA-1
tig0020460 CDS 34119 34304 34119 ID=Msa1381900-mRNA-1:cds;Parent=Msa1381900-mRNA-1
tig0020460 CDS 33897 33995 33897 ID=Msa1381900-mRNA-1:cds;Parent=Msa1381900-mRNA-1
tig0020460 CDS 33553 33669 33553 ID=Msa1381900-mRNA-1:cds;Parent=Msa1381900-mRNA-1
tig0020460 CDS 33322 33417 33322 ID=Msa1381900-mRNA-1:cds;Parent=Msa1381900-mRNA-1
tig0020460 CDS 33092 33204 33092 ID=Msa1381900-mRNA-1:cds;Parent=Msa1381900-mRNA-1
tig0020460 CDS 32833 32947 32833 ID=Msa1381900-mRNA-1:cds;Parent=Msa1381900-mRNA-1
tig0020460 three_prime_UTR 32562 32832 32562 ID=Msa1381900-mRNA-1:three_prime_utr;Parent=Msa1381900-mRNA-1
Gene Sequence

>Msa1381900

CGATATGCGTACACAATAGATACGAAACTAATTGAGCTTTCAAAATTGAAACAGGTACCTCCTCCAGGGAAAGGGACGCTTTATCTTAGGCCTTTGCTGATAGGAACAGGAGCTGCATTAGGCTTGGCTCCATCACCTGAGTACACATTTCTCATTTATTGCTCCCCTGTTGGAAATTACCACGAGGGAGGAAGACTAAACTTAAAAGTGGAGGATAAATTTCATCGATCAATAGCTGGCAGTGGTGGAACAGGAGGAATCAAGAGTGTTACTAATTATGCCCCGATATATACTGCAGTAACTGAAGCAAAAGCCAATGGATTTTCTGATGTCTTGTTCTTGGATTCAGCAACTGGAAACAATATTGAGGAGGCTACTGCATGCAATATATTTGTTGTGAAGGAAAATGATATCTTCACTCCGGCAATAGATGGATCTATTCTGCCTGGGGTCACACGAAAATCCATCATAGACATCGCCATTGATTTGGGTTATAAGGTCATAGAACGTTCCATATCAGTGGAGGAAATGATGAGTGCTGATGAAGTGTTCTGCACAGGAACTGCAGTGGTTGTTACCTCTGTTGCATCTGTAACATATAAGGAAACAAGAGCTGAATATAAAACAGGCGCAGAAACGTTGTCTGAAAAACTACGAGGAATACTGGTTGGAATACAAACAGGGCGTATTGAGGACAAAAAGTCATGGACAGTCCAAGTAGATTGA
Protein sequence

>Msa1381900

RYAYTIDTKLIELSKLKQVPPPGKGTLYLRPLLIGTGAALGLAPSPEYTFLIYCSPVGNYHEGGRLNLKVEDKFHRSIAGSGGTGGIKSVTNYAPIYTAVTEAKANGFSDVLFLDSATGNNIEEATACNIFVVKENDIFTPAIDGSILPGVTRKSIIDIAIDLGYKVIERSISVEEMMSADEVFCTGTAVVVTSVASVTYKETRAEYKTGAETLSEKLRGILVGIQTGRIEDKKSWTVQVD*