AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0656260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0656260 MtrunA17_Chr8g0376881 89.194 546 46 4 1 541 1 538 0.0 990
Msa0656260 MtrunA17_Chr3g0123131 62.522 571 163 10 12 541 1 561 0.0 659
Msa0656260 MtrunA17_Chr1g0161431 57.249 269 110 2 25 288 11 279 1.11e-95 303
Msa0656260 MtrunA17_Chr4g0067981 59.690 258 100 1 23 276 29 286 2.70e-93 299
Msa0656260 MtrunA17_Chr2g0294911 57.143 252 106 1 23 274 27 276 1.03e-87 282
Msa0656260 MtrunA17_Chr3g0137101 54.373 263 108 2 23 276 6 265 1.20e-86 280
Msa0656260 MtrunA17_Chr1g0149841 55.303 264 96 4 23 274 3 256 6.10e-81 261
Msa0656260 MtrunA17_Chr4g0074411 50.958 261 101 4 23 270 16 262 5.59e-76 251
Msa0656260 MtrunA17_Chr2g0304841 47.152 316 127 6 22 298 15 329 1.55e-75 252
Msa0656260 MtrunA17_Chr4g0074401 52.016 248 117 1 25 270 17 264 7.65e-75 248
Msa0656260 MtrunA17_Chr1g0149851 50.936 267 102 4 23 266 3 263 1.12e-70 236
Msa0656260 MtrunA17_Chr7g0275891 45.833 288 121 5 23 285 3 280 6.46e-69 225
Msa0656260 MtrunA17_Chr7g0275401 50.196 255 104 5 23 264 3 247 7.29e-66 215
Msa0656260 MtrunA17_Chr3g0134481 47.059 238 66 2 23 260 6 183 3.70e-60 197
Msa0656260 MtrunA17_Chr4g0009851 41.085 258 121 6 24 281 10 236 7.49e-58 195
Msa0656260 MtrunA17_Chr4g0009901 42.798 243 113 4 24 266 9 225 2.18e-56 190
Msa0656260 MtrunA17_Chr4g0074421 51.042 192 85 4 75 263 2 187 9.36e-55 183
Msa0656260 MtrunA17_Chr1g0149831 51.724 203 73 5 84 274 1 190 1.27e-51 176
Msa0656260 MtrunA17_Chr3g0134461 46.414 237 76 3 23 258 6 192 4.45e-50 171
Msa0656260 MtrunA17_Chr4g0009981 39.252 214 100 4 70 283 2 185 5.26e-43 154
Msa0656260 MtrunA17_Chr6g0471041 36.777 242 123 6 25 264 14 227 5.72e-43 158
Msa0656260 MtrunA17_Chr8g0391911 34.156 243 146 4 23 258 7 242 6.35e-34 129
Msa0656260 MtrunA17_Chr8g0383961 40.000 150 89 1 25 173 14 163 7.41e-28 112
Msa0656260 MtrunA17_Chr4g0011071 33.129 163 84 2 72 234 36 173 8.07e-27 107
Msa0656260 MtrunA17_Chr6g0473731 34.158 202 106 5 105 303 10 187 8.00e-26 110
Msa0656260 MtrunA17_Chr8g0350761 29.412 255 158 7 27 261 19 271 3.44e-24 105
Msa0656260 MtrunA17_Chr6g0456631 34.266 143 86 4 25 160 9 150 3.57e-23 96.7
Msa0656260 MtrunA17_Chr3g0127941 35.673 171 95 5 29 194 56 216 1.83e-22 101
Msa0656260 MtrunA17_Chr7g0276361 37.500 128 71 2 26 147 11 135 1.52e-20 95.5
Msa0656260 MtrunA17_Chr4g0009991 36.620 142 64 3 117 258 1 116 3.37e-20 86.7
Msa0656260 MtrunA17_Chr4g0030271 36.585 123 73 2 29 147 89 210 5.03e-19 90.9
Msa0656260 MtrunA17_Chr3g0091641 34.146 123 78 1 29 148 24 146 1.81e-18 89.0
Msa0656260 MtrunA17_Chr8g0345901 36.585 123 73 2 31 149 38 159 5.65e-18 87.4
Msa0656260 MtrunA17_Chr4g0031961 58.333 72 29 1 207 277 129 200 6.10e-18 84.7
Msa0656260 MtrunA17_Chr1g0181811 36.066 122 75 1 29 147 85 206 1.38e-17 86.3
Msa0656260 MtrunA17_Chr4g0061021 34.959 123 77 1 29 148 33 155 1.48e-17 85.9
Msa0656260 MtrunA17_Chr5g0423031 41.463 82 47 1 24 105 9 89 7.61e-17 79.0
Msa0656260 MtrunA17_Chr7g0255711 59.677 62 25 0 207 268 89 150 8.02e-17 81.3
Msa0656260 MtrunA17_Chr4g0010001 41.463 82 47 1 24 105 9 89 5.12e-15 70.9
Msa0656260 MtrunA17_Chr4g0054981 54.795 73 32 1 208 279 309 381 7.76e-15 77.4
Msa0656260 MtrunA17_Chr4g0054981 35.484 124 76 2 25 147 14 134 1.68e-13 73.2
Msa0656260 MtrunA17_Chr5g0400241 62.500 56 21 0 208 263 310 365 1.43e-14 76.6
Msa0656260 MtrunA17_Chr5g0400241 36.800 125 73 3 25 147 14 134 1.90e-13 72.8
Msa0656260 MtrunA17_Chr2g0324891 46.377 69 37 0 208 276 160 228 2.34e-13 72.0
Msa0656260 MtrunA17_Chr4g0040481 46.377 69 37 0 208 276 157 225 3.09e-13 71.6
Msa0656260 MtrunA17_Chr8g0352871 29.861 144 81 3 31 154 11 154 1.03e-12 66.6
Msa0656260 MtrunA17_Chr4g0059571 29.577 142 85 4 26 152 20 161 1.33e-12 67.4
Msa0656260 MtrunA17_Chr6g0469321 75.000 44 11 0 224 267 69 112 1.76e-12 65.9
Msa0656260 MtrunA17_Chr7g0256551 25.294 170 104 3 24 170 4 173 7.22e-12 65.5
Msa0656260 MtrunA17_Chr1g0198601 46.479 71 37 1 208 277 210 280 1.32e-11 66.2
Msa0656260 MtrunA17_Chr6g0461641 53.448 58 26 1 209 265 42 99 1.51e-11 66.2
Msa0656260 MtrunA17_Chr2g0321461 46.667 75 33 2 209 282 45 113 1.96e-11 65.9
Msa0656260 MtrunA17_Chr5g0417521 52.727 55 25 1 209 262 15 69 2.93e-11 65.1
Msa0656260 MtrunA17_Chr4g0062921 53.333 60 27 1 208 266 234 293 3.06e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0656260 AT1G67710.1 49.398 498 199 14 26 506 8 469 3.38e-127 382
Msa0656260 AT1G67710.2 45.227 440 188 14 84 506 1 404 9.35e-92 288
Msa0656260 AT3G16857.1 53.289 304 115 7 22 302 30 329 1.37e-86 281
Msa0656260 AT3G16857.2 53.289 304 115 7 22 302 30 329 2.45e-86 281
Msa0656260 AT2G01760.1 52.416 269 107 4 20 273 2 264 1.67e-82 262
Msa0656260 AT2G25180.1 54.826 259 103 2 22 274 10 260 5.44e-82 267
Msa0656260 AT2G01760.2 47.475 297 107 5 20 273 2 292 3.65e-76 246
Msa0656260 AT4G31920.1 45.912 318 125 7 22 312 10 307 3.02e-75 248
Msa0656260 AT1G67710.3 42.381 420 189 14 104 506 12 395 3.76e-75 245
Msa0656260 AT3G16857.3 52.290 262 98 7 64 302 2 259 8.41e-68 230
Msa0656260 AT5G58080.1 50.000 258 117 4 23 276 12 261 2.23e-65 224
Msa0656260 AT2G01760.3 50.732 205 80 4 84 273 1 199 1.07e-56 192
Msa0656260 AT5G49240.1 39.407 236 140 2 25 258 38 272 6.68e-47 166
Msa0656260 AT1G49190.1 33.083 266 145 5 22 268 27 278 5.64e-38 144
Msa0656260 AT5G07210.2 32.601 273 148 7 29 270 16 283 3.15e-34 137
Msa0656260 AT3G62670.1 35.417 240 134 6 30 261 42 268 9.95e-33 130
Msa0656260 AT5G07210.1 31.673 281 148 9 29 270 16 291 1.91e-32 132
Msa0656260 AT2G27070.1 30.584 291 159 6 29 283 16 299 5.64e-31 127
Msa0656260 AT1G49190.2 41.406 128 75 0 22 149 27 154 1.55e-26 114
Msa0656260 AT5G24470.1 38.400 125 72 2 28 148 49 172 1.24e-21 99.0
Msa0656260 AT2G46790.3 38.211 123 71 2 29 147 37 158 1.80e-21 97.8
Msa0656260 AT2G46790.1 38.211 123 71 2 29 147 37 158 2.16e-21 97.8
Msa0656260 AT5G60100.7 39.855 138 77 3 29 162 64 199 5.08e-20 92.8
Msa0656260 AT5G60100.6 39.855 138 77 3 29 162 64 199 5.08e-20 92.8
Msa0656260 AT5G60100.4 39.855 138 77 3 29 162 64 199 5.08e-20 92.8
Msa0656260 AT1G68210.1 25.091 275 163 4 29 267 13 280 6.33e-20 94.4
Msa0656260 AT5G60100.2 40.310 129 71 3 29 153 64 190 6.66e-20 93.6
Msa0656260 AT5G60100.5 39.855 138 77 3 29 162 64 199 8.17e-20 93.2
Msa0656260 AT5G60100.3 39.855 138 77 3 29 162 64 199 8.17e-20 93.2
Msa0656260 AT5G60100.1 39.855 138 77 3 29 162 64 199 8.17e-20 93.2
Msa0656260 AT5G61380.1 36.585 123 75 1 29 148 19 141 1.53e-18 89.7
Msa0656260 AT3G46640.2 50.633 79 38 1 199 277 135 212 2.04e-17 84.0
Msa0656260 AT3G46640.1 50.633 79 38 1 199 277 135 212 2.04e-17 84.0
Msa0656260 AT3G46640.3 53.521 71 33 0 207 277 142 212 3.22e-17 83.2
Msa0656260 AT2G40970.1 59.677 62 25 0 207 268 103 164 3.70e-17 81.6
Msa0656260 AT5G59570.2 60.656 61 24 0 208 268 140 200 5.64e-17 82.0
Msa0656260 AT5G59570.1 60.656 61 24 0 208 268 140 200 5.64e-17 82.0
Msa0656260 AT5G02810.1 38.211 123 71 2 29 147 78 199 6.23e-17 84.7
Msa0656260 AT5G05090.1 46.667 105 53 2 207 311 79 180 7.23e-17 81.3
Msa0656260 AT3G10760.1 59.677 62 25 0 207 268 103 164 7.66e-17 82.4
Msa0656260 AT5G44190.2 50.000 76 38 0 208 283 146 221 3.90e-15 77.4
Msa0656260 AT5G44190.1 50.000 76 38 0 208 283 146 221 4.08e-15 77.8
Msa0656260 AT2G20570.1 48.000 75 39 0 208 282 152 226 2.03e-14 75.9
Msa0656260 AT2G20570.2 45.783 83 45 0 200 282 160 242 5.48e-14 74.7
Msa0656260 AT3G57040.2 28.931 159 90 4 31 167 11 168 9.77e-14 71.2
Msa0656260 AT3G57040.1 28.931 159 90 4 31 167 11 168 9.77e-14 71.2
Msa0656260 AT4G18020.8 34.074 135 80 3 25 152 14 146 5.32e-13 71.6
Msa0656260 AT4G18020.8 50.769 65 32 0 209 273 296 360 8.88e-13 71.2
Msa0656260 AT4G18020.11 34.074 135 80 3 25 152 14 146 5.32e-13 71.6
Msa0656260 AT4G18020.11 50.769 65 32 0 209 273 296 360 8.88e-13 71.2
Msa0656260 AT4G18020.4 34.074 135 80 3 25 152 14 146 5.32e-13 71.6
Msa0656260 AT4G18020.4 50.769 65 32 0 209 273 296 360 8.88e-13 71.2
Msa0656260 AT4G18020.5 34.074 135 80 3 25 152 14 146 5.32e-13 71.6
Msa0656260 AT4G18020.5 50.769 65 32 0 209 273 296 360 8.88e-13 71.2
Msa0656260 AT4G18020.10 34.074 135 80 3 25 152 14 146 5.32e-13 71.6
Msa0656260 AT4G18020.10 50.769 65 32 0 209 273 296 360 8.88e-13 71.2
Msa0656260 AT4G18020.7 34.074 135 80 3 25 152 14 146 5.32e-13 71.6
Msa0656260 AT4G18020.7 50.769 65 32 0 209 273 296 360 8.88e-13 71.2
Msa0656260 AT4G18020.3 34.074 135 80 3 25 152 14 146 7.68e-13 71.2
Msa0656260 AT4G18020.3 50.769 65 32 0 209 273 296 360 7.81e-13 71.2
Msa0656260 AT4G18020.2 34.074 135 80 3 25 152 14 146 7.68e-13 71.2
Msa0656260 AT4G18020.2 50.769 65 32 0 209 273 296 360 7.81e-13 71.2
Msa0656260 AT4G18020.1 34.074 135 80 3 25 152 14 146 7.68e-13 71.2
Msa0656260 AT4G18020.1 50.769 65 32 0 209 273 296 360 7.81e-13 71.2
Msa0656260 AT4G18020.6 34.074 135 80 3 25 152 14 146 7.68e-13 71.2
Msa0656260 AT4G18020.6 50.769 65 32 0 209 273 296 360 7.81e-13 71.2
Msa0656260 AT4G18020.9 34.074 135 80 3 25 152 14 146 7.68e-13 71.2
Msa0656260 AT4G18020.9 50.769 65 32 0 209 273 296 360 7.81e-13 71.2
Msa0656260 AT5G18240.2 56.364 55 23 1 209 262 45 99 7.52e-12 67.8
Msa0656260 AT5G18240.3 56.364 55 23 1 209 262 45 99 7.52e-12 67.8
Msa0656260 AT5G18240.5 56.364 55 23 1 209 262 45 99 8.15e-12 67.8
Msa0656260 AT5G18240.1 56.364 55 23 1 209 262 45 99 8.67e-12 67.4
Msa0656260 AT5G18240.4 56.364 55 23 1 209 262 45 99 8.67e-12 67.4
Msa0656260 AT3G04030.1 54.545 55 24 1 209 262 45 99 1.12e-11 67.0
Msa0656260 AT3G04030.3 54.545 55 24 1 209 262 45 99 1.25e-11 67.0

Find 84 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGGTTGCATTTATGATA+TGG 0.215616 4_3:+65529485 Msa0656260:CDS
TGTTGCTGTGTAACATTTAA+TGG 0.220612 4_3:-65529270 None:intergenic
TTGAAAGTGATTGGTCAATT+TGG 0.229447 4_3:-65529296 None:intergenic
ACCTCTGAACTCTTTATCTT+TGG 0.235951 4_3:-65529096 None:intergenic
ATCATTTGAGATGAGGCTTT+TGG 0.254966 4_3:-65529406 None:intergenic
GAACACACTTGTACAATTAA+AGG 0.258335 4_3:+65529230 Msa0656260:CDS
ATTATTTGCTTAAGCCTATA+AGG 0.266042 4_3:+65528032 Msa0656260:CDS
CGCAATTCTTTCATCCTTAT+AGG 0.272142 4_3:-65528046 None:intergenic
TACCCAAAAGTAAGTTTCTT+TGG 0.274466 4_3:-65529515 None:intergenic
CTACCAAAGAAACTTACTTT+TGG 0.276715 4_3:+65529512 Msa0656260:CDS
TGGAATTAATTGAAAGTGAT+TGG 0.324987 4_3:-65529305 None:intergenic
TTTGAAGTTAAAATGATATC+AGG 0.326062 4_3:-65529435 None:intergenic
AAGTTCGATCTACGAGTTCT+TGG 0.327718 4_3:-65528249 None:intergenic
GAAGGTGCAATCGATAGTTT+TGG 0.347432 4_3:+65528991 Msa0656260:CDS
ATCTGCTGAAGCATACAAAT+TGG 0.351333 4_3:-65529673 None:intergenic
TGAGTCAGCTTCCACACCTT+TGG 0.366813 4_3:+65529539 Msa0656260:CDS
AAATGTTGCTAGTCACTTGC+AGG 0.372643 4_3:+65528587 Msa0656260:CDS
TAAAGAAAGCTAGAGTAGTT+TGG 0.378977 4_3:+65528275 Msa0656260:CDS
AATGTAATCATTCTTGTAAT+CGG 0.392849 4_3:-65529334 None:intergenic
GGAATTCACTTAATGAGAAA+TGG 0.405209 4_3:+65528133 Msa0656260:CDS
TTACTTCTGATTCCAGAGAT+TGG 0.407149 4_3:-65529128 None:intergenic
AAAACACCAAGATTTAATCT+TGG 0.410977 4_3:+65529468 Msa0656260:CDS
CACATGTTGCTTAGCAATAG+AGG 0.420963 4_3:+65527610 Msa0656260:CDS
CCATACCAACACAGTACTCT+TGG 0.432925 4_3:+65529172 Msa0656260:CDS
ATTCTTGTAATCGGAGGGAT+TGG 0.437838 4_3:-65529325 None:intergenic
CATATGCATCTTTACTTTCG+CGG 0.445631 4_3:-65527646 None:intergenic
AGCTTGAAACCATCCATGTC+AGG 0.458164 4_3:-65527695 None:intergenic
GGTAGGTCTTAACCAATCTC+TGG 0.459702 4_3:+65529116 Msa0656260:CDS
CATATTCACCTTGTAACAAA+AGG 0.468727 4_3:-65529578 None:intergenic
AAATGAACTTAATGAAGAAA+AGG 0.469526 4_3:+65528200 Msa0656260:CDS
ATGCAACCAAGATTAAATCT+TGG 0.480002 4_3:-65529474 None:intergenic
TGAAAGAATTGCGAAACATA+TGG 0.480046 4_3:+65528056 Msa0656260:CDS
GATTTACCTTCAAAAGATCC+AGG 0.483223 4_3:+65528928 Msa0656260:CDS
TTCAAGCTTCTTGAACATGT+TGG 0.489231 4_3:+65527710 Msa0656260:CDS
TTGAGAAAGCTTAAACATCC+TGG 0.491396 4_3:-65528946 None:intergenic
CAAACATCATGGTACACAAC+AGG 0.500098 4_3:+65526912 None:intergenic
TATGCAGTGATGTCTGTTGA+TGG 0.505268 4_3:+65527965 Msa0656260:intron
AGCTTCCACACCTTTGGACG+AGG 0.506847 4_3:+65529545 Msa0656260:CDS
GTTAAAGCTGTGAATCAACT+TGG 0.507253 4_3:+65528322 Msa0656260:CDS
GGATCAGATCACACTGATGA+TGG 0.509373 4_3:+65528154 Msa0656260:CDS
ACGAGAACTCGTAGACCAGC+TGG 0.523070 4_3:-65526995 None:intergenic
CATTAGTCCTGTGAATTCAT+CGG 0.523463 4_3:+65529353 Msa0656260:CDS
TGAACATGTTGGACTTGAGA+TGG 0.525184 4_3:+65527721 Msa0656260:CDS
AAAATTCAAATGGATACTAC+AGG 0.527020 4_3:+65529033 Msa0656260:CDS
GAGATTGATCATTTGAGATG+AGG 0.529752 4_3:-65529413 None:intergenic
GTGTAACATTTAATGGCAAA+TGG 0.530650 4_3:-65529263 None:intergenic
GGAACTTCAGCAATAAACCT+TGG 0.534763 4_3:-65529648 None:intergenic
TAAAAGAACTTACTAATGAC+AGG 0.538868 4_3:-65527749 None:intergenic
GTGTTCTGTTCTTCTTCGAG+TGG 0.552522 4_3:-65529213 None:intergenic
CGATGTAAACATGCCTGACA+TGG 0.555999 4_3:+65527682 Msa0656260:CDS
GCAACCTACTCAAGTATAGC+CGG 0.557803 4_3:-65528858 None:intergenic
TAATCATTCTTGTAATCGGA+GGG 0.562863 4_3:-65529330 None:intergenic
TGGTGGAGTCAAATGCTGTA+TGG 0.564365 4_3:-65529152 None:intergenic
AAGATAAAGAGTTCAGAGGT+AGG 0.566025 4_3:+65529099 Msa0656260:CDS
AAAGCATCAAGGTGTGGAGA+AGG 0.568756 4_3:-65526971 None:intergenic
CCAAGAGTACTGTGTTGGTA+TGG 0.569363 4_3:-65529172 None:intergenic
AATGATCAGAAATTGTCCTC+AGG 0.572148 4_3:+65528889 Msa0656260:CDS
GCTGAAGCATACAAATTGGT+AGG 0.582128 4_3:-65529669 None:intergenic
TCATTCCAAGAGTACTGTGT+TGG 0.583367 4_3:-65529177 None:intergenic
TGTCAGAGTATTATGATCCA+AGG 0.585355 4_3:+65529631 Msa0656260:CDS
CTGTTGATGGAGAAACAAGC+AGG 0.593651 4_3:+65527978 Msa0656260:CDS
GTGATGAAAGGTGTTCAACA+TGG 0.594525 4_3:+65528001 Msa0656260:CDS
GTAATCATTCTTGTAATCGG+AGG 0.596210 4_3:-65529331 None:intergenic
AAGTTTAAGTCCTCGTCCAA+AGG 0.599539 4_3:-65529555 None:intergenic
AGAGTACTGTGTTGGTATGG+TGG 0.600173 4_3:-65529169 None:intergenic
GACCAGCTGGAAAAGCATCA+AGG 0.602271 4_3:-65526982 None:intergenic
TTGTTGATGATGATCTAACT+TGG 0.602339 4_3:+65527017 Msa0656260:CDS
AGCTAGAGTAGTTTGGTCTG+TGG 0.615227 4_3:+65528282 Msa0656260:CDS
GAAGACACCGATGAATTCAC+AGG 0.619719 4_3:-65529360 None:intergenic
TCCAAAGATAAAGAGTTCAG+AGG 0.620214 4_3:+65529095 Msa0656260:CDS
AATACCGGCTATACTTGAGT+AGG 0.620491 4_3:+65528854 Msa0656260:CDS
CTCTATTGCTAAGCAACATG+TGG 0.620636 4_3:-65527609 None:intergenic
TAAGTCCTCGTCCAAAGGTG+TGG 0.621176 4_3:-65529550 None:intergenic
GAAACAAGCAGGGTGATGAA+AGG 0.627020 4_3:+65527989 Msa0656260:CDS
GCTGGAAAAGCATCAAGGTG+TGG 0.628160 4_3:-65526977 None:intergenic
GTCTCTTGTCTATGACAACA+TGG 0.628850 4_3:+65526934 Msa0656260:CDS
AAGAGAATTTGAAAGTCTCG+AGG 0.633493 4_3:+65528111 Msa0656260:CDS
TGAATGCTTTATTCTACCTG+AGG 0.641744 4_3:-65528905 None:intergenic
GATGGTAACATGTTTGCTGT+AGG 0.648059 4_3:+65528172 Msa0656260:CDS
TAGATTTGATGAATGTTCCG+TGG 0.652142 4_3:+65528553 Msa0656260:CDS
TTCAGCAAGCAACAAAACGA+AGG 0.652173 4_3:+65528973 Msa0656260:CDS
AGAGAATTTGAAAGTCTCGA+GGG 0.654792 4_3:+65528112 Msa0656260:CDS
GTAAACATGCCTGACATGGA+TGG 0.678855 4_3:+65527686 Msa0656260:CDS
TGTTGATGGAGAAACAAGCA+GGG 0.723682 4_3:+65527979 Msa0656260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCTTTGAAAAAAAAAAAA+AGG - chr4_3:65527259-65527278 None:intergenic 10.0%
!!! GTTTTTTTTTAGTATAAATT+AGG - chr4_3:65527496-65527515 None:intergenic 10.0%
!!! TGATAAAAATAATTTCTTTT+TGG + chr4_3:65528506-65528525 Msa0656260:intron 10.0%
!!! ATTTTTTTAGCATTTTTTCA+AGG - chr4_3:65527051-65527070 None:intergenic 15.0%
!! TGAAAAAAAAAAAAAGGAAT+TGG - chr4_3:65527253-65527272 None:intergenic 15.0%
!!! TCTTTTAACTTAATTAACAA+AGG + chr4_3:65527361-65527380 Msa0656260:intron 15.0%
!!! TTTCTTAAATTTGCAAATTT+TGG + chr4_3:65527529-65527548 Msa0656260:intron 15.0%
!!! AAGAGCAAAATTTTATTATT+AGG - chr4_3:65528830-65528849 None:intergenic 15.0%
!!! AAGTGTTGTTTGTAAAATAA+TGG - chr4_3:65527937-65527956 None:intergenic 20.0%
!! AAATGAACTTAATGAAGAAA+AGG + chr4_3:65528200-65528219 Msa0656260:CDS 20.0%
!!! ATCAAATCTAGAATTTTCTT+GGG - chr4_3:65528543-65528562 None:intergenic 20.0%
!!! TAAACTTTTAACTTTAGTAG+AGG - chr4_3:65528634-65528653 None:intergenic 20.0%
!! AATGTAATCATTCTTGTAAT+CGG - chr4_3:65529337-65529356 None:intergenic 20.0%
!!! TTTGAAGTTAAAATGATATC+AGG - chr4_3:65529438-65529457 None:intergenic 20.0%
! TTGAATTGTACTGAACTTAT+TGG - chr4_3:65527177-65527196 None:intergenic 25.0%
! TAAAAGAACTTACTAATGAC+AGG - chr4_3:65527752-65527771 None:intergenic 25.0%
!!! TGTCTTCTAGCTTTTTTTTT+CGG - chr4_3:65527866-65527885 None:intergenic 25.0%
! ATTATTTGCTTAAGCCTATA+AGG + chr4_3:65528032-65528051 Msa0656260:CDS 25.0%
! CTTAAGAAAAAGAATACATG+AGG + chr4_3:65528087-65528106 Msa0656260:CDS 25.0%
!!! CATCAAATCTAGAATTTTCT+TGG - chr4_3:65528544-65528563 None:intergenic 25.0%
!!! AATATTTCTGAGATGTGTAT+TGG - chr4_3:65528740-65528759 None:intergenic 25.0%
!!! TTTTGAGTTTGAAAACTTAC+AGG + chr4_3:65528802-65528821 Msa0656260:intron 25.0%
!!! ATAGTTTTGGATATTCAGAT+GGG + chr4_3:65529004-65529023 Msa0656260:CDS 25.0%
! TGGGACATTAAAAATTCAAA+TGG + chr4_3:65529023-65529042 Msa0656260:CDS 25.0%
! AAAATTCAAATGGATACTAC+AGG + chr4_3:65529033-65529052 Msa0656260:CDS 25.0%
! TGGAATTAATTGAAAGTGAT+TGG - chr4_3:65529308-65529327 None:intergenic 25.0%
! AAAACACCAAGATTTAATCT+TGG + chr4_3:65529468-65529487 Msa0656260:CDS 25.0%
!!! TACCAAAGAAACTTACTTTT+GGG + chr4_3:65529513-65529532 Msa0656260:CDS 25.0%
!! TTAAACTTCCTTTTGTTACA+AGG + chr4_3:65529570-65529589 Msa0656260:CDS 25.0%
!! TTGTTGATGATGATCTAACT+TGG + chr4_3:65527017-65527036 Msa0656260:CDS 30.0%
! CTAAAAAAATGCTTTTACCG+AGG + chr4_3:65527061-65527080 Msa0656260:CDS 30.0%
TGAATGAAAGAAAGAAACCT+CGG - chr4_3:65527081-65527100 None:intergenic 30.0%
!! TATATACCACTTTTAGCACA+TGG - chr4_3:65527124-65527143 None:intergenic 30.0%
ATTAGAAGCCAACAAAAATG+TGG - chr4_3:65527228-65527247 None:intergenic 30.0%
GTACCTACTAGTAATATTCT+TGG + chr4_3:65527839-65527858 Msa0656260:intron 30.0%
TGAAAGAATTGCGAAACATA+TGG + chr4_3:65528056-65528075 Msa0656260:CDS 30.0%
GGAATTCACTTAATGAGAAA+TGG + chr4_3:65528133-65528152 Msa0656260:CDS 30.0%
TAAAGAAAGCTAGAGTAGTT+TGG + chr4_3:65528275-65528294 Msa0656260:CDS 30.0%
GTAGATTTATTTAGTGTGCT+TGG - chr4_3:65528412-65528431 None:intergenic 30.0%
CTCTTACACAAAAGATATGA+TGG - chr4_3:65528479-65528498 None:intergenic 30.0%
!! AATTTCTTTTTGGCAGAAGT+TGG + chr4_3:65528516-65528535 Msa0656260:intron 30.0%
!! TCTGATATGTTGTTTCTGTT+AGG + chr4_3:65528670-65528689 Msa0656260:intron 30.0%
! ATTTTGCTCTTCAGAAATAC+CGG + chr4_3:65528839-65528858 Msa0656260:intron 30.0%
!!! GATAGTTTTGGATATTCAGA+TGG + chr4_3:65529003-65529022 Msa0656260:CDS 30.0%
GAACACACTTGTACAATTAA+AGG + chr4_3:65529230-65529249 Msa0656260:CDS 30.0%
GTGTAACATTTAATGGCAAA+TGG - chr4_3:65529266-65529285 None:intergenic 30.0%
TGTTGCTGTGTAACATTTAA+TGG - chr4_3:65529273-65529292 None:intergenic 30.0%
! TTGAAAGTGATTGGTCAATT+TGG - chr4_3:65529299-65529318 None:intergenic 30.0%
TAATCATTCTTGTAATCGGA+GGG - chr4_3:65529333-65529352 None:intergenic 30.0%
ATGCAACCAAGATTAAATCT+TGG - chr4_3:65529477-65529496 None:intergenic 30.0%
TCTTGGTTGCATTTATGATA+TGG + chr4_3:65529485-65529504 Msa0656260:CDS 30.0%
! CTACCAAAGAAACTTACTTT+TGG + chr4_3:65529512-65529531 Msa0656260:CDS 30.0%
TACCCAAAAGTAAGTTTCTT+TGG - chr4_3:65529518-65529537 None:intergenic 30.0%
CATATTCACCTTGTAACAAA+AGG - chr4_3:65529581-65529600 None:intergenic 30.0%
! TCACCAATCCACATTTTTGT+TGG + chr4_3:65527217-65527236 Msa0656260:intron 35.0%
AAGCCAACAAAAATGTGGAT+TGG - chr4_3:65527223-65527242 None:intergenic 35.0%
CTAGGATGAGATGATAATAC+TGG + chr4_3:65527336-65527355 Msa0656260:intron 35.0%
! TACGACTCATGTGTCTTATT+TGG + chr4_3:65527433-65527452 Msa0656260:intron 35.0%
CATATGCATCTTTACTTTCG+CGG - chr4_3:65527649-65527668 None:intergenic 35.0%
TTCAAGCTTCTTGAACATGT+TGG + chr4_3:65527710-65527729 Msa0656260:CDS 35.0%
!! TATTACTAGTAGGTACTGAC+TGG - chr4_3:65527835-65527854 None:intergenic 35.0%
GGACCAAGAATATTACTAGT+AGG - chr4_3:65527845-65527864 None:intergenic 35.0%
CGCAATTCTTTCATCCTTAT+AGG - chr4_3:65528049-65528068 None:intergenic 35.0%
AAGAGAATTTGAAAGTCTCG+AGG + chr4_3:65528111-65528130 Msa0656260:CDS 35.0%
AGAGAATTTGAAAGTCTCGA+GGG + chr4_3:65528112-65528131 Msa0656260:CDS 35.0%
GTTAAAGCTGTGAATCAACT+TGG + chr4_3:65528322-65528341 Msa0656260:CDS 35.0%
TAGATTTGATGAATGTTCCG+TGG + chr4_3:65528553-65528572 Msa0656260:CDS 35.0%
AATGATCAGAAATTGTCCTC+AGG + chr4_3:65528889-65528908 Msa0656260:CDS 35.0%
! TGAATGCTTTATTCTACCTG+AGG - chr4_3:65528908-65528927 None:intergenic 35.0%
GATTTACCTTCAAAAGATCC+AGG + chr4_3:65528928-65528947 Msa0656260:CDS 35.0%
! AAACATCCTGGATCTTTTGA+AGG - chr4_3:65528937-65528956 None:intergenic 35.0%
TTGAGAAAGCTTAAACATCC+TGG - chr4_3:65528949-65528968 None:intergenic 35.0%
TCCAAAGATAAAGAGTTCAG+AGG + chr4_3:65529095-65529114 Msa0656260:CDS 35.0%
ACCTCTGAACTCTTTATCTT+TGG - chr4_3:65529099-65529118 None:intergenic 35.0%
AAGATAAAGAGTTCAGAGGT+AGG + chr4_3:65529099-65529118 Msa0656260:CDS 35.0%
TTACTTCTGATTCCAGAGAT+TGG - chr4_3:65529131-65529150 None:intergenic 35.0%
GTAATCATTCTTGTAATCGG+AGG - chr4_3:65529334-65529353 None:intergenic 35.0%
CATTAGTCCTGTGAATTCAT+CGG + chr4_3:65529353-65529372 Msa0656260:CDS 35.0%
!! ATCATTTGAGATGAGGCTTT+TGG - chr4_3:65529409-65529428 None:intergenic 35.0%
! GAGATTGATCATTTGAGATG+AGG - chr4_3:65529416-65529435 None:intergenic 35.0%
! TGTCAGAGTATTATGATCCA+AGG + chr4_3:65529631-65529650 Msa0656260:CDS 35.0%
ATCTGCTGAAGCATACAAAT+TGG - chr4_3:65529676-65529695 None:intergenic 35.0%
GTCTCTTGTCTATGACAACA+TGG + chr4_3:65526934-65526953 Msa0656260:CDS 40.0%
CTTCTTCCATGTGCTAAAAG+TGG + chr4_3:65527115-65527134 Msa0656260:intron 40.0%
ACTAACCAATAACGCTAGCT+AGG + chr4_3:65527318-65527337 Msa0656260:intron 40.0%
! TAGTCTTAATAGCTGCGTGA+TGG + chr4_3:65527466-65527485 Msa0656260:intron 40.0%
AGTCTTAATAGCTGCGTGAT+GGG + chr4_3:65527467-65527486 Msa0656260:intron 40.0%
CTCTATTGCTAAGCAACATG+TGG - chr4_3:65527612-65527631 None:intergenic 40.0%
! CACATGTTGCTTAGCAATAG+AGG + chr4_3:65527610-65527629 Msa0656260:CDS 40.0%
TGAACATGTTGGACTTGAGA+TGG + chr4_3:65527721-65527740 Msa0656260:CDS 40.0%
TATGCAGTGATGTCTGTTGA+TGG + chr4_3:65527965-65527984 Msa0656260:intron 40.0%
! TGTTGATGGAGAAACAAGCA+GGG + chr4_3:65527979-65527998 Msa0656260:CDS 40.0%
!! GTGATGAAAGGTGTTCAACA+TGG + chr4_3:65528001-65528020 Msa0656260:CDS 40.0%
!! GATGGTAACATGTTTGCTGT+AGG + chr4_3:65528172-65528191 Msa0656260:CDS 40.0%
AAGTTCGATCTACGAGTTCT+TGG - chr4_3:65528252-65528271 None:intergenic 40.0%
! ACATTTTCTCTAGTCAGCCA+CGG - chr4_3:65528573-65528592 None:intergenic 40.0%
AAATGTTGCTAGTCACTTGC+AGG + chr4_3:65528587-65528606 Msa0656260:CDS 40.0%
! AATACCGGCTATACTTGAGT+AGG + chr4_3:65528854-65528873 Msa0656260:CDS 40.0%
TTCAGCAAGCAACAAAACGA+AGG + chr4_3:65528973-65528992 Msa0656260:CDS 40.0%
!! GAAGGTGCAATCGATAGTTT+TGG + chr4_3:65528991-65529010 Msa0656260:CDS 40.0%
!! TCATTCCAAGAGTACTGTGT+TGG - chr4_3:65529180-65529199 None:intergenic 40.0%
ATTCTTGTAATCGGAGGGAT+TGG - chr4_3:65529328-65529347 None:intergenic 40.0%
AAGTTTAAGTCCTCGTCCAA+AGG - chr4_3:65529558-65529577 None:intergenic 40.0%
GGAACTTCAGCAATAAACCT+TGG - chr4_3:65529651-65529670 None:intergenic 40.0%
GCTGAAGCATACAAATTGGT+AGG - chr4_3:65529672-65529691 None:intergenic 40.0%
! AAAGCATCAAGGTGTGGAGA+AGG - chr4_3:65526974-65526993 None:intergenic 45.0%
CTCATCCTAGCTAGCGTTAT+TGG - chr4_3:65527326-65527345 None:intergenic 45.0%
CGATGTAAACATGCCTGACA+TGG + chr4_3:65527682-65527701 Msa0656260:CDS 45.0%
GTAAACATGCCTGACATGGA+TGG + chr4_3:65527686-65527705 Msa0656260:CDS 45.0%
AGCTTGAAACCATCCATGTC+AGG - chr4_3:65527698-65527717 None:intergenic 45.0%
! CTGTTGATGGAGAAACAAGC+AGG + chr4_3:65527978-65527997 Msa0656260:CDS 45.0%
GAAACAAGCAGGGTGATGAA+AGG + chr4_3:65527989-65528008 Msa0656260:CDS 45.0%
GGATCAGATCACACTGATGA+TGG + chr4_3:65528154-65528173 Msa0656260:CDS 45.0%
AGCTAGAGTAGTTTGGTCTG+TGG + chr4_3:65528282-65528301 Msa0656260:CDS 45.0%
TCTGTTAGGCCTAGAACTAG+AGG + chr4_3:65528684-65528703 Msa0656260:intron 45.0%
CTGTTAGGCCTAGAACTAGA+GGG + chr4_3:65528685-65528704 Msa0656260:intron 45.0%
! CTAGAACTAGAGGGCTAGTA+GGG + chr4_3:65528694-65528713 Msa0656260:intron 45.0%
! TAGAACTAGAGGGCTAGTAG+GGG + chr4_3:65528695-65528714 Msa0656260:intron 45.0%
GCAACCTACTCAAGTATAGC+CGG - chr4_3:65528861-65528880 None:intergenic 45.0%
GGTAGGTCTTAACCAATCTC+TGG + chr4_3:65529116-65529135 Msa0656260:CDS 45.0%
TGGTGGAGTCAAATGCTGTA+TGG - chr4_3:65529155-65529174 None:intergenic 45.0%
!! AGAGTACTGTGTTGGTATGG+TGG - chr4_3:65529172-65529191 None:intergenic 45.0%
!! CCAAGAGTACTGTGTTGGTA+TGG - chr4_3:65529175-65529194 None:intergenic 45.0%
CCATACCAACACAGTACTCT+TGG + chr4_3:65529172-65529191 Msa0656260:CDS 45.0%
GTGTTCTGTTCTTCTTCGAG+TGG - chr4_3:65529216-65529235 None:intergenic 45.0%
GAAGACACCGATGAATTCAC+AGG - chr4_3:65529363-65529382 None:intergenic 45.0%
! GCTTTTGGACTGACTAGGAA+AGG - chr4_3:65529394-65529413 None:intergenic 45.0%
! ATGAGGCTTTTGGACTGACT+AGG - chr4_3:65529399-65529418 None:intergenic 45.0%
!! GCTGGAAAAGCATCAAGGTG+TGG - chr4_3:65526980-65526999 None:intergenic 50.0%
! CACCTTGATGCTTTTCCAGC+TGG + chr4_3:65526980-65526999 Msa0656260:CDS 50.0%
!! GACCAGCTGGAAAAGCATCA+AGG - chr4_3:65526985-65527004 None:intergenic 50.0%
!!! CCTCTCCCAGCTCTTTTTTC+CGG + chr4_3:65527808-65527827 Msa0656260:intron 50.0%
CTGGTCCGGAAAAAAGAGCT+GGG - chr4_3:65527816-65527835 None:intergenic 50.0%
ACTGGTCCGGAAAAAAGAGC+TGG - chr4_3:65527817-65527836 None:intergenic 50.0%
!! CTAGTAGGTACTGACTGGTC+CGG - chr4_3:65527830-65527849 None:intergenic 50.0%
!! CCTACTAGCCCTCTAGTTCT+AGG - chr4_3:65528696-65528715 None:intergenic 50.0%
! CCTAGAACTAGAGGGCTAGT+AGG + chr4_3:65528693-65528712 Msa0656260:intron 50.0%
TGAGTCAGCTTCCACACCTT+TGG + chr4_3:65529539-65529558 Msa0656260:CDS 50.0%
TAAGTCCTCGTCCAAAGGTG+TGG - chr4_3:65529553-65529572 None:intergenic 50.0%
ACGAGAACTCGTAGACCAGC+TGG - chr4_3:65526998-65527017 None:intergenic 55.0%
CCGGAAAAAAGAGCTGGGAG+AGG - chr4_3:65527811-65527830 None:intergenic 55.0%
AGCTTCCACACCTTTGGACG+AGG + chr4_3:65529545-65529564 Msa0656260:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 65526920 65529704 65526920 ID=Msa0656260;Name=Msa0656260
chr4_3 mRNA 65526920 65529704 65526920 ID=Msa0656260-mRNA-1;Parent=Msa0656260;Name=Msa0656260-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|5|0|541
chr4_3 exon 65526920 65527082 65526920 ID=Msa0656260-mRNA-1:exon:18569;Parent=Msa0656260-mRNA-1
chr4_3 exon 65527606 65527758 65527606 ID=Msa0656260-mRNA-1:exon:18570;Parent=Msa0656260-mRNA-1
chr4_3 exon 65527972 65528352 65527972 ID=Msa0656260-mRNA-1:exon:18571;Parent=Msa0656260-mRNA-1
chr4_3 exon 65528532 65528608 65528532 ID=Msa0656260-mRNA-1:exon:18572;Parent=Msa0656260-mRNA-1
chr4_3 exon 65528853 65529704 65528853 ID=Msa0656260-mRNA-1:exon:18573;Parent=Msa0656260-mRNA-1
chr4_3 CDS 65526920 65527082 65526920 ID=Msa0656260-mRNA-1:cds;Parent=Msa0656260-mRNA-1
chr4_3 CDS 65527606 65527758 65527606 ID=Msa0656260-mRNA-1:cds;Parent=Msa0656260-mRNA-1
chr4_3 CDS 65527972 65528352 65527972 ID=Msa0656260-mRNA-1:cds;Parent=Msa0656260-mRNA-1
chr4_3 CDS 65528532 65528608 65528532 ID=Msa0656260-mRNA-1:cds;Parent=Msa0656260-mRNA-1
chr4_3 CDS 65528853 65529704 65528853 ID=Msa0656260-mRNA-1:cds;Parent=Msa0656260-mRNA-1
Gene Sequence

>Msa0656260

ATGGTACACAACAGGTCTCTTGTCTATGACAACATGGATAATTCTTGCTTTCCTTCTCCACACCTTGATGCTTTTCCAGCTGGTCTACGAGTTCTCGTTGTTGATGATGATCTAACTTGGCTCAGAATCCTTGAAAAAATGCTAAAAAAATGCTTTTACCGAGTGACCACATGTTGCTTAGCAATAGAGGCTCTTAAGAAGCTCCGCGAAAGTAAAGATGCATATGACATAGTGATCAGCGATGTAAACATGCCTGACATGGATGGTTTCAAGCTTCTTGAACATGTTGGACTTGAGATGGATCTTCCTGTCATTATGATGTCTGTTGATGGAGAAACAAGCAGGGTGATGAAAGGTGTTCAACATGGAGCATGTGATTATTTGCTTAAGCCTATAAGGATGAAAGAATTGCGAAACATATGGCAACATGTCTTAAGAAAAAGAATACATGAGGCAAGAGAATTTGAAAGTCTCGAGGGAATTCACTTAATGAGAAATGGATCAGATCACACTGATGATGGTAACATGTTTGCTGTAGGAAAAGAAATGAACTTAATGAAGAAAAGGAAAAATTTCGATAGTAAACATGATGACCAAGAACTCGTAGATCGAACTTTCATAAAGAAAGCTAGAGTAGTTTGGTCTGTGGATCTTCATCAGAAGTTTGTTAAAGCTGTGAATCAACTTGGATTCGATAAAGTTGGTCCCAAGAAAATTCTAGATTTGATGAATGTTCCGTGGCTGACTAGAGAAAATGTTGCTAGTCACTTGCAGAAATACCGGCTATACTTGAGTAGGTTGCAAAAGAAAAATGATCAGAAATTGTCCTCAGGTAGAATAAAGCATTCAGATTTACCTTCAAAAGATCCAGGATGTTTAAGCTTTCTCAACTCTTTCAGCAAGCAACAAAACGAAGGTGCAATCGATAGTTTTGGATATTCAGATGGGACATTAAAAATTCAAATGGATACTACAGGTCATGAAAAAAGAAGAGCTTCAACTAGTGAAATTTCTGATCCAAAGATAAAGAGTTCAGAGGTAGGTCTTAACCAATCTCTGGAATCAGAAGTAAACCATACAGCATTTGACTCCACCATACCAACACAGTACTCTTGGAATGAAGTTTCTAAAACGCCACTCGAAGAAGAACAGAACACACTTGTACAATTAAAGGATAGCTTCAGCCATTTGCCATTAAATGTTACACAGCAACACTTCCAAATTGACCAATCACTTTCAATTAATTCCAATCCCTCCGATTACAAGAATGATTACATTAGTCCTGTGAATTCATCGGTGTCTTCAGTTGATACCTTTCCTAGTCAGTCCAAAAGCCTCATCTCAAATGATCAATCTCCTGATATCATTTTAACTTCAAATTTGAGTTCAAAAACACCAAGATTTAATCTTGGTTGCATTTATGATATGGAAATCTACCAAAGAAACTTACTTTTGGGTATTGAGTCAGCTTCCACACCTTTGGACGAGGACTTAAACTTCCTTTTGTTACAAGGTGAATATGATATGAACTTTGACATGCAGAATATAGAGATGTCAGAGTATTATGATCCAAGGTTTATTGCTGAAGTTCCTACCAATTTGTATGCTTCAGCAGATTATTCTTAG
Protein sequence

>Msa0656260

MVHNRSLVYDNMDNSCFPSPHLDAFPAGLRVLVVDDDLTWLRILEKMLKKCFYRVTTCCLAIEALKKLRESKDAYDIVISDVNMPDMDGFKLLEHVGLEMDLPVIMMSVDGETSRVMKGVQHGACDYLLKPIRMKELRNIWQHVLRKRIHEAREFESLEGIHLMRNGSDHTDDGNMFAVGKEMNLMKKRKNFDSKHDDQELVDRTFIKKARVVWSVDLHQKFVKAVNQLGFDKVGPKKILDLMNVPWLTRENVASHLQKYRLYLSRLQKKNDQKLSSGRIKHSDLPSKDPGCLSFLNSFSKQQNEGAIDSFGYSDGTLKIQMDTTGHEKRRASTSEISDPKIKSSEVGLNQSLESEVNHTAFDSTIPTQYSWNEVSKTPLEEEQNTLVQLKDSFSHLPLNVTQQHFQIDQSLSINSNPSDYKNDYISPVNSSVSSVDTFPSQSKSLISNDQSPDIILTSNLSSKTPRFNLGCIYDMEIYQRNLLLGIESASTPLDEDLNFLLLQGEYDMNFDMQNIEMSEYYDPRFIAEVPTNLYASADYS*