AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0700060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0700060 MtrunA17_Chr8g0376881 89.377 546 46 3 1 542 1 538 0.0 997
Msa0700060 MtrunA17_Chr3g0123131 62.413 572 163 10 12 542 1 561 0.0 659
Msa0700060 MtrunA17_Chr1g0161431 57.249 269 110 2 25 288 11 279 1.31e-95 303
Msa0700060 MtrunA17_Chr4g0067981 59.690 258 100 1 23 276 29 286 3.12e-93 299
Msa0700060 MtrunA17_Chr2g0294911 57.143 252 106 1 23 274 27 276 1.04e-87 282
Msa0700060 MtrunA17_Chr3g0137101 54.373 263 108 2 23 276 6 265 1.25e-86 280
Msa0700060 MtrunA17_Chr1g0149841 55.303 264 96 4 23 274 3 256 5.23e-81 261
Msa0700060 MtrunA17_Chr4g0074411 50.958 261 101 4 23 270 16 262 6.51e-76 251
Msa0700060 MtrunA17_Chr2g0304841 47.152 316 127 6 22 298 15 329 1.76e-75 252
Msa0700060 MtrunA17_Chr4g0074401 52.016 248 117 1 25 270 17 264 1.05e-74 248
Msa0700060 MtrunA17_Chr1g0149851 50.936 267 102 4 23 266 3 263 1.21e-70 236
Msa0700060 MtrunA17_Chr7g0275891 46.181 288 120 6 23 285 3 280 5.58e-69 226
Msa0700060 MtrunA17_Chr7g0275401 50.196 255 104 5 23 264 3 247 7.22e-66 216
Msa0700060 MtrunA17_Chr3g0134481 47.059 238 66 2 23 260 6 183 3.86e-60 197
Msa0700060 MtrunA17_Chr4g0009851 41.085 258 121 6 24 281 10 236 8.06e-58 195
Msa0700060 MtrunA17_Chr4g0009901 42.798 243 113 4 24 266 9 225 2.42e-56 190
Msa0700060 MtrunA17_Chr4g0074421 51.042 192 85 4 75 263 2 187 9.35e-55 183
Msa0700060 MtrunA17_Chr1g0149831 51.724 203 73 5 84 274 1 190 1.25e-51 176
Msa0700060 MtrunA17_Chr3g0134461 46.414 237 76 3 23 258 6 192 4.48e-50 171
Msa0700060 MtrunA17_Chr4g0009981 39.252 214 100 4 70 283 2 185 5.73e-43 154
Msa0700060 MtrunA17_Chr6g0471041 36.777 242 123 6 25 264 14 227 6.55e-43 158
Msa0700060 MtrunA17_Chr8g0391911 34.156 243 146 4 23 258 7 242 7.02e-34 129
Msa0700060 MtrunA17_Chr8g0383961 40.000 150 89 1 25 173 14 163 6.57e-28 112
Msa0700060 MtrunA17_Chr4g0011071 33.129 163 84 2 72 234 36 173 8.11e-27 107
Msa0700060 MtrunA17_Chr6g0473731 34.158 202 106 5 105 303 10 187 8.45e-26 110
Msa0700060 MtrunA17_Chr8g0350761 29.412 255 158 7 27 261 19 271 3.25e-24 105
Msa0700060 MtrunA17_Chr6g0456631 34.266 143 86 4 25 160 9 150 3.35e-23 96.7
Msa0700060 MtrunA17_Chr3g0127941 35.673 171 95 5 29 194 56 216 1.96e-22 101
Msa0700060 MtrunA17_Chr7g0276361 37.500 128 71 2 26 147 11 135 1.56e-20 95.5
Msa0700060 MtrunA17_Chr4g0009991 36.620 142 64 3 117 258 1 116 3.38e-20 86.7
Msa0700060 MtrunA17_Chr4g0030271 36.585 123 73 2 29 147 89 210 4.85e-19 90.9
Msa0700060 MtrunA17_Chr3g0091641 34.146 123 78 1 29 148 24 146 1.90e-18 88.6
Msa0700060 MtrunA17_Chr8g0345901 36.585 123 73 2 31 149 38 159 5.78e-18 87.4
Msa0700060 MtrunA17_Chr4g0031961 58.333 72 29 1 207 277 129 200 6.35e-18 84.7
Msa0700060 MtrunA17_Chr1g0181811 36.066 122 75 1 29 147 85 206 1.32e-17 86.7
Msa0700060 MtrunA17_Chr4g0061021 34.959 123 77 1 29 148 33 155 1.51e-17 85.9
Msa0700060 MtrunA17_Chr5g0423031 41.463 82 47 1 24 105 9 89 7.27e-17 79.0
Msa0700060 MtrunA17_Chr7g0255711 59.677 62 25 0 207 268 89 150 8.35e-17 81.3
Msa0700060 MtrunA17_Chr4g0010001 41.463 82 47 1 24 105 9 89 5.03e-15 70.9
Msa0700060 MtrunA17_Chr4g0054981 54.795 73 32 1 208 279 309 381 8.22e-15 77.4
Msa0700060 MtrunA17_Chr4g0054981 35.484 124 76 2 25 147 14 134 1.67e-13 73.2
Msa0700060 MtrunA17_Chr5g0400241 62.500 56 21 0 208 263 310 365 1.46e-14 76.3
Msa0700060 MtrunA17_Chr5g0400241 36.800 125 73 3 25 147 14 134 1.96e-13 72.8
Msa0700060 MtrunA17_Chr2g0324891 46.377 69 37 0 208 276 160 228 2.33e-13 72.0
Msa0700060 MtrunA17_Chr4g0040481 46.377 69 37 0 208 276 157 225 3.36e-13 71.6
Msa0700060 MtrunA17_Chr8g0352871 29.861 144 81 3 31 154 11 154 9.35e-13 66.6
Msa0700060 MtrunA17_Chr4g0059571 29.577 142 85 4 26 152 20 161 1.24e-12 67.4
Msa0700060 MtrunA17_Chr6g0469321 75.000 44 11 0 224 267 69 112 1.76e-12 65.9
Msa0700060 MtrunA17_Chr7g0256551 25.294 170 104 3 24 170 4 173 7.66e-12 65.5
Msa0700060 MtrunA17_Chr1g0198601 46.479 71 37 1 208 277 210 280 1.39e-11 66.2
Msa0700060 MtrunA17_Chr6g0461641 54.545 55 24 1 209 262 42 96 1.50e-11 66.2
Msa0700060 MtrunA17_Chr2g0321461 46.667 75 33 2 209 282 45 113 1.91e-11 65.9
Msa0700060 MtrunA17_Chr5g0417521 52.727 55 25 1 209 262 15 69 3.02e-11 65.1
Msa0700060 MtrunA17_Chr4g0062921 53.333 60 27 1 208 266 234 293 3.32e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0700060 AT1G67710.1 49.299 499 199 15 26 507 8 469 6.67e-126 379
Msa0700060 AT1G67710.2 45.125 441 188 15 84 507 1 404 1.27e-90 286
Msa0700060 AT3G16857.1 53.289 304 115 7 22 302 30 329 1.58e-86 281
Msa0700060 AT3G16857.2 53.289 304 115 7 22 302 30 329 3.08e-86 281
Msa0700060 AT2G01760.1 52.416 269 107 4 20 273 2 264 1.83e-82 262
Msa0700060 AT2G25180.1 54.826 259 103 2 22 274 10 260 6.41e-82 267
Msa0700060 AT2G01760.2 47.475 297 107 5 20 273 2 292 4.30e-76 246
Msa0700060 AT4G31920.1 45.912 318 125 7 22 312 10 307 3.48e-75 248
Msa0700060 AT1G67710.3 42.280 421 189 15 104 507 12 395 6.87e-74 242
Msa0700060 AT3G16857.3 52.290 262 98 7 64 302 2 259 9.86e-68 230
Msa0700060 AT5G58080.1 50.000 258 117 4 23 276 12 261 2.30e-65 224
Msa0700060 AT2G01760.3 50.732 205 80 4 84 273 1 199 1.05e-56 192
Msa0700060 AT5G49240.1 39.407 236 140 2 25 258 38 272 7.53e-47 166
Msa0700060 AT1G49190.1 33.083 266 145 5 22 268 27 278 5.02e-38 145
Msa0700060 AT5G07210.2 32.601 273 148 7 29 270 16 283 3.68e-34 137
Msa0700060 AT3G62670.1 35.417 240 134 6 30 261 42 268 1.07e-32 130
Msa0700060 AT5G07210.1 31.673 281 148 9 29 270 16 291 1.97e-32 132
Msa0700060 AT2G27070.1 30.584 291 159 6 29 283 16 299 6.08e-31 127
Msa0700060 AT1G49190.2 41.406 128 75 0 22 149 27 154 1.42e-26 114
Msa0700060 AT5G24470.1 38.400 125 72 2 28 148 49 172 1.27e-21 99.0
Msa0700060 AT2G46790.3 38.211 123 71 2 29 147 37 158 1.86e-21 97.8
Msa0700060 AT2G46790.1 38.211 123 71 2 29 147 37 158 2.27e-21 97.4
Msa0700060 AT5G60100.7 39.855 138 77 3 29 162 64 199 4.65e-20 93.2
Msa0700060 AT5G60100.6 39.855 138 77 3 29 162 64 199 4.65e-20 93.2
Msa0700060 AT5G60100.4 39.855 138 77 3 29 162 64 199 4.65e-20 93.2
Msa0700060 AT1G68210.1 25.091 275 163 4 29 267 13 280 6.09e-20 94.4
Msa0700060 AT5G60100.2 40.310 129 71 3 29 153 64 190 6.88e-20 93.6
Msa0700060 AT5G60100.5 39.855 138 77 3 29 162 64 199 8.21e-20 93.2
Msa0700060 AT5G60100.3 39.855 138 77 3 29 162 64 199 8.21e-20 93.2
Msa0700060 AT5G60100.1 39.855 138 77 3 29 162 64 199 8.21e-20 93.2
Msa0700060 AT5G61380.1 36.585 123 75 1 29 148 19 141 1.48e-18 89.7
Msa0700060 AT3G46640.2 50.633 79 38 1 199 277 135 212 2.10e-17 84.0
Msa0700060 AT3G46640.1 50.633 79 38 1 199 277 135 212 2.10e-17 84.0
Msa0700060 AT3G46640.3 53.521 71 33 0 207 277 142 212 3.14e-17 83.6
Msa0700060 AT2G40970.1 59.677 62 25 0 207 268 103 164 3.82e-17 81.6
Msa0700060 AT5G59570.2 60.656 61 24 0 208 268 140 200 5.81e-17 82.0
Msa0700060 AT5G59570.1 60.656 61 24 0 208 268 140 200 5.81e-17 82.0
Msa0700060 AT5G02810.1 38.211 123 71 2 29 147 78 199 6.38e-17 84.7
Msa0700060 AT5G05090.1 46.667 105 53 2 207 311 79 180 7.32e-17 81.3
Msa0700060 AT3G10760.1 59.677 62 25 0 207 268 103 164 7.34e-17 82.4
Msa0700060 AT5G44190.2 50.000 76 38 0 208 283 146 221 4.17e-15 77.4
Msa0700060 AT5G44190.1 50.000 76 38 0 208 283 146 221 4.37e-15 77.8
Msa0700060 AT2G20570.1 48.000 75 39 0 208 282 152 226 2.19e-14 75.9
Msa0700060 AT2G20570.2 45.783 83 45 0 200 282 160 242 5.91e-14 74.7
Msa0700060 AT3G57040.2 28.313 166 96 4 24 167 4 168 9.17e-14 71.6
Msa0700060 AT3G57040.1 28.313 166 96 4 24 167 4 168 9.17e-14 71.6
Msa0700060 AT4G18020.8 34.074 135 80 3 25 152 14 146 4.68e-13 72.0
Msa0700060 AT4G18020.8 50.769 65 32 0 209 273 296 360 9.07e-13 70.9
Msa0700060 AT4G18020.11 34.074 135 80 3 25 152 14 146 4.68e-13 72.0
Msa0700060 AT4G18020.11 50.769 65 32 0 209 273 296 360 9.07e-13 70.9
Msa0700060 AT4G18020.4 34.074 135 80 3 25 152 14 146 4.68e-13 72.0
Msa0700060 AT4G18020.4 50.769 65 32 0 209 273 296 360 9.07e-13 70.9
Msa0700060 AT4G18020.5 34.074 135 80 3 25 152 14 146 4.68e-13 72.0
Msa0700060 AT4G18020.5 50.769 65 32 0 209 273 296 360 9.07e-13 70.9
Msa0700060 AT4G18020.10 34.074 135 80 3 25 152 14 146 4.68e-13 72.0
Msa0700060 AT4G18020.10 50.769 65 32 0 209 273 296 360 9.07e-13 70.9
Msa0700060 AT4G18020.7 34.074 135 80 3 25 152 14 146 4.68e-13 72.0
Msa0700060 AT4G18020.7 50.769 65 32 0 209 273 296 360 9.07e-13 70.9
Msa0700060 AT4G18020.3 34.074 135 80 3 25 152 14 146 7.31e-13 71.6
Msa0700060 AT4G18020.3 50.769 65 32 0 209 273 296 360 8.27e-13 71.2
Msa0700060 AT4G18020.2 34.074 135 80 3 25 152 14 146 7.31e-13 71.6
Msa0700060 AT4G18020.2 50.769 65 32 0 209 273 296 360 8.27e-13 71.2
Msa0700060 AT4G18020.1 34.074 135 80 3 25 152 14 146 7.31e-13 71.6
Msa0700060 AT4G18020.1 50.769 65 32 0 209 273 296 360 8.27e-13 71.2
Msa0700060 AT4G18020.6 34.074 135 80 3 25 152 14 146 7.31e-13 71.6
Msa0700060 AT4G18020.6 50.769 65 32 0 209 273 296 360 8.27e-13 71.2
Msa0700060 AT4G18020.9 34.074 135 80 3 25 152 14 146 7.31e-13 71.6
Msa0700060 AT4G18020.9 50.769 65 32 0 209 273 296 360 8.27e-13 71.2
Msa0700060 AT5G18240.2 56.364 55 23 1 209 262 45 99 7.61e-12 67.8
Msa0700060 AT5G18240.3 56.364 55 23 1 209 262 45 99 7.61e-12 67.8
Msa0700060 AT5G18240.5 56.364 55 23 1 209 262 45 99 8.17e-12 67.8
Msa0700060 AT5G18240.1 56.364 55 23 1 209 262 45 99 8.55e-12 67.8
Msa0700060 AT5G18240.4 56.364 55 23 1 209 262 45 99 8.55e-12 67.8
Msa0700060 AT3G04030.1 54.545 55 24 1 209 262 45 99 1.11e-11 67.0
Msa0700060 AT3G04030.3 54.545 55 24 1 209 262 45 99 1.30e-11 67.0

Find 84 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGAACTCTTTCTTATCTT+TGG 0.198401 4_4:-70940603 None:intergenic
TCTTGGTTGCATTTATGATA+TGG 0.215616 4_4:+70940995 Msa0700060:CDS
TGTTGCTGTGTAACATTTAA+TGG 0.220612 4_4:-70940780 None:intergenic
TTGAAAGTGATTGGTCAATT+TGG 0.229447 4_4:-70940806 None:intergenic
ATCATTTGAGATGAGGCTTT+TGG 0.254966 4_4:-70940916 None:intergenic
GAACACACTTGTACAATTAA+AGG 0.258335 4_4:+70940740 Msa0700060:CDS
ATTATTTGCTTAAGCCTATA+AGG 0.266042 4_4:+70939536 Msa0700060:CDS
CGCAATTCTTTCATCCTTAT+AGG 0.272142 4_4:-70939550 None:intergenic
TACCCAAAAGTAAGTTTCTT+TGG 0.274466 4_4:-70941025 None:intergenic
CTACCAAAGAAACTTACTTT+TGG 0.276715 4_4:+70941022 Msa0700060:CDS
TGGAATTAATTGAAAGTGAT+TGG 0.324987 4_4:-70940815 None:intergenic
TTTGAAGTTAAAATGATATC+AGG 0.326062 4_4:-70940945 None:intergenic
AAGTTCGATCTACGAGTTCT+TGG 0.327718 4_4:-70939753 None:intergenic
GAAGGTGCAATCGATAGTTT+TGG 0.347432 4_4:+70940498 Msa0700060:CDS
ATCTGCTGAAGCATACAAAT+TGG 0.351333 4_4:-70941183 None:intergenic
TGAGTCAGCTTCCACACCTT+TGG 0.366813 4_4:+70941049 Msa0700060:CDS
AAATGTTGCTAGTCACTTGC+AGG 0.372643 4_4:+70940094 Msa0700060:CDS
TAAAGAAAGCTAGAGTAGTT+TGG 0.378977 4_4:+70939779 Msa0700060:CDS
AATGTAATCATTCTTGTAAT+CGG 0.392849 4_4:-70940844 None:intergenic
GGAATTCACTTAATGAGAAA+TGG 0.405209 4_4:+70939637 Msa0700060:CDS
TTACTTCTGATTCCAGAGAT+TGG 0.407149 4_4:-70940638 None:intergenic
AAAACACCAAGATTTAATCT+TGG 0.410977 4_4:+70940978 Msa0700060:CDS
CACATGTTGCTTAGCAATAG+AGG 0.420963 4_4:+70939114 Msa0700060:CDS
CCATACCAACACAGTACTCT+TGG 0.432925 4_4:+70940682 Msa0700060:CDS
ATTCTTGTAATCGGAGGGAT+TGG 0.437838 4_4:-70940835 None:intergenic
CATATGCATCTTTACTTTCG+CGG 0.445631 4_4:-70939150 None:intergenic
AGCTTGAAACCATCCATGTC+AGG 0.458164 4_4:-70939199 None:intergenic
GGTAGGTCTTAACCAATCTC+TGG 0.459702 4_4:+70940626 Msa0700060:CDS
CATATTCACCTTGTAACAAA+AGG 0.468727 4_4:-70941088 None:intergenic
AAATGAACTTAATGAAGAAA+AGG 0.469526 4_4:+70939704 Msa0700060:CDS
ATGCAACCAAGATTAAATCT+TGG 0.480002 4_4:-70940984 None:intergenic
TGAAAGAATTGCGAAACATA+TGG 0.480046 4_4:+70939560 Msa0700060:CDS
GATTTACCTTCAAAAGATCC+AGG 0.483223 4_4:+70940435 Msa0700060:CDS
TTCAAGCTTCTTGAACATGT+TGG 0.489231 4_4:+70939214 Msa0700060:CDS
TTGAGAAAGCTTAAACATCC+TGG 0.491396 4_4:-70940453 None:intergenic
CAAACATCATGGTACACAAC+AGG 0.500098 4_4:+70938416 None:intergenic
TATGCAGTGATGTCTGTTGA+TGG 0.505268 4_4:+70939469 Msa0700060:intron
AGCTTCCACACCTTTGGACG+AGG 0.506847 4_4:+70941055 Msa0700060:CDS
GTTAAAGCTGTGAATCAACT+TGG 0.507253 4_4:+70939826 Msa0700060:CDS
GGATCAGATCACACTGATGA+TGG 0.509373 4_4:+70939658 Msa0700060:CDS
ACGAGAACTCGTAGACCAGC+TGG 0.523070 4_4:-70938499 None:intergenic
CATTAGTCCTGTGAATTCAT+CGG 0.523463 4_4:+70940863 Msa0700060:CDS
TAAAAGAACTTACTAATGAC+AGG 0.524293 4_4:-70939253 None:intergenic
TGAACATGTTGGACTTGAGA+TGG 0.525184 4_4:+70939225 Msa0700060:CDS
AAAATTCAAATGGATACTAC+AGG 0.527020 4_4:+70940540 Msa0700060:CDS
GAGATTGATCATTTGAGATG+AGG 0.529752 4_4:-70940923 None:intergenic
GTGTAACATTTAATGGCAAA+TGG 0.530650 4_4:-70940773 None:intergenic
GGAACTTCAGCAATAAACCT+TGG 0.534763 4_4:-70941158 None:intergenic
GTGTTCTGTTCTTCTTCGAG+TGG 0.552522 4_4:-70940723 None:intergenic
CGATGTAAACATGCCTGACA+TGG 0.555999 4_4:+70939186 Msa0700060:CDS
GCAACCTACTCAAGTATAGC+CGG 0.557803 4_4:-70940365 None:intergenic
TAATCATTCTTGTAATCGGA+GGG 0.562863 4_4:-70940840 None:intergenic
TGGTGGAGTCAAATGCTGTA+TGG 0.564365 4_4:-70940662 None:intergenic
AAAGCATCAAGGTGTGGAGA+AGG 0.568756 4_4:-70938475 None:intergenic
CCAAGAGTACTGTGTTGGTA+TGG 0.569363 4_4:-70940682 None:intergenic
AATGATCAGAAATTGTCCTC+AGG 0.572148 4_4:+70940396 Msa0700060:CDS
GCTGAAGCATACAAATTGGT+AGG 0.582128 4_4:-70941179 None:intergenic
TCATTCCAAGAGTACTGTGT+TGG 0.583367 4_4:-70940687 None:intergenic
TGTCAGAGTATTATGATCCA+AGG 0.585355 4_4:+70941141 Msa0700060:CDS
CTGTTGATGGAGAAACAAGC+AGG 0.593651 4_4:+70939482 Msa0700060:CDS
GTGATGAAAGGTGTTCAACA+TGG 0.594525 4_4:+70939505 Msa0700060:CDS
GTAATCATTCTTGTAATCGG+AGG 0.596210 4_4:-70940841 None:intergenic
ATAAGAAAGAGTTCAGAGGT+AGG 0.597933 4_4:+70940609 Msa0700060:CDS
AAGTTTAAGTCCTCGTCCAA+AGG 0.599539 4_4:-70941065 None:intergenic
AGAGTACTGTGTTGGTATGG+TGG 0.600173 4_4:-70940679 None:intergenic
GACCAGCTGGAAAAGCATCA+AGG 0.602271 4_4:-70938486 None:intergenic
TTGTTGATGATGATCTAACT+TGG 0.602339 4_4:+70938521 Msa0700060:CDS
AGCTAGAGTAGTTTGGTCTG+TGG 0.615227 4_4:+70939786 Msa0700060:CDS
GAAGACACCGATGAATTCAC+AGG 0.619719 4_4:-70940870 None:intergenic
AATACCGGCTATACTTGAGT+AGG 0.620491 4_4:+70940361 Msa0700060:CDS
CTCTATTGCTAAGCAACATG+TGG 0.620636 4_4:-70939113 None:intergenic
TAAGTCCTCGTCCAAAGGTG+TGG 0.621176 4_4:-70941060 None:intergenic
AAAGATAAGAAAGAGTTCAG+AGG 0.625719 4_4:+70940605 Msa0700060:CDS
GAAACAAGCAGGGTGATGAA+AGG 0.627020 4_4:+70939493 Msa0700060:CDS
GCTGGAAAAGCATCAAGGTG+TGG 0.628160 4_4:-70938481 None:intergenic
GTCTCTTGTCTATGACAACA+TGG 0.628850 4_4:+70938438 Msa0700060:CDS
AAGAGAATTTGAAAGTCTCG+AGG 0.633493 4_4:+70939615 Msa0700060:CDS
TGAATGCTTTATTCTACCTG+AGG 0.641744 4_4:-70940412 None:intergenic
GATGGTAACATGTTTGCTGT+AGG 0.648059 4_4:+70939676 Msa0700060:CDS
TAGATTTGATGAATGTTCCG+TGG 0.652142 4_4:+70940060 Msa0700060:CDS
TTCAGCAAGCAACAAAACGA+AGG 0.652173 4_4:+70940480 Msa0700060:CDS
AGAGAATTTGAAAGTCTCGA+GGG 0.654792 4_4:+70939616 Msa0700060:CDS
GTAAACATGCCTGACATGGA+TGG 0.678855 4_4:+70939190 Msa0700060:CDS
TGTTGATGGAGAAACAAGCA+GGG 0.723682 4_4:+70939483 Msa0700060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCTTTGAAAAAAAAAAAA+AGG - chr4_4:70938763-70938782 None:intergenic 10.0%
!!! GTTTTTTTTTAGTATAAATT+AGG - chr4_4:70939000-70939019 None:intergenic 10.0%
!!! TGATAAAAATAATTTCTTTT+TGG + chr4_4:70940013-70940032 Msa0700060:intron 10.0%
!!! ATTTTTTTAGCATTTTTTCA+AGG - chr4_4:70938555-70938574 None:intergenic 15.0%
!! TGAAAAAAAAAAAAAGGAAT+TGG - chr4_4:70938757-70938776 None:intergenic 15.0%
!!! TCTTTTAACTTAATTAACAA+AGG + chr4_4:70938865-70938884 Msa0700060:intron 15.0%
!!! TTTCTTAAATTTGCAAATTT+TGG + chr4_4:70939033-70939052 Msa0700060:intron 15.0%
!!! AAGAGCAAAATTTTATTATT+AGG - chr4_4:70940337-70940356 None:intergenic 15.0%
!!! AAGTGTTGTTTGTAAAATAA+TGG - chr4_4:70939441-70939460 None:intergenic 20.0%
!! AAATGAACTTAATGAAGAAA+AGG + chr4_4:70939704-70939723 Msa0700060:CDS 20.0%
!!! ATCAAATCTAGAATTTTCTT+GGG - chr4_4:70940050-70940069 None:intergenic 20.0%
!!! TAAACTTTTAACTTTAGTAG+AGG - chr4_4:70940141-70940160 None:intergenic 20.0%
!! AATGTAATCATTCTTGTAAT+CGG - chr4_4:70940847-70940866 None:intergenic 20.0%
!!! TTTGAAGTTAAAATGATATC+AGG - chr4_4:70940948-70940967 None:intergenic 20.0%
! TTGAATTGTACTGAACTTAT+TGG - chr4_4:70938681-70938700 None:intergenic 25.0%
! TAAAAGAACTTACTAATGAC+AGG - chr4_4:70939256-70939275 None:intergenic 25.0%
! ATTATTTGCTTAAGCCTATA+AGG + chr4_4:70939536-70939555 Msa0700060:CDS 25.0%
! CTTAAGAAAAAGAATACATG+AGG + chr4_4:70939591-70939610 Msa0700060:CDS 25.0%
!!! CATCAAATCTAGAATTTTCT+TGG - chr4_4:70940051-70940070 None:intergenic 25.0%
!!! AATATTTCTGAGATGTGTAT+TGG - chr4_4:70940247-70940266 None:intergenic 25.0%
!!! TTTTGAGTTTGAAAACTTAC+AGG + chr4_4:70940309-70940328 Msa0700060:intron 25.0%
!!! ATAGTTTTGGATATTCAGAT+GGG + chr4_4:70940511-70940530 Msa0700060:CDS 25.0%
! TGGGACATTAAAAATTCAAA+TGG + chr4_4:70940530-70940549 Msa0700060:CDS 25.0%
! AAAATTCAAATGGATACTAC+AGG + chr4_4:70940540-70940559 Msa0700060:CDS 25.0%
! TGGAATTAATTGAAAGTGAT+TGG - chr4_4:70940818-70940837 None:intergenic 25.0%
! AAAACACCAAGATTTAATCT+TGG + chr4_4:70940978-70940997 Msa0700060:CDS 25.0%
!!! TACCAAAGAAACTTACTTTT+GGG + chr4_4:70941023-70941042 Msa0700060:CDS 25.0%
!! TTAAACTTCCTTTTGTTACA+AGG + chr4_4:70941080-70941099 Msa0700060:CDS 25.0%
!! TTGTTGATGATGATCTAACT+TGG + chr4_4:70938521-70938540 Msa0700060:CDS 30.0%
! CTAAAAAAATGCTTTTACCG+AGG + chr4_4:70938565-70938584 Msa0700060:CDS 30.0%
TGAATGAAAGAAAGAAACCT+CGG - chr4_4:70938585-70938604 None:intergenic 30.0%
!! TATATACCACTTTTAGCACA+TGG - chr4_4:70938628-70938647 None:intergenic 30.0%
ATTAGAAGCCAACAAAAATG+TGG - chr4_4:70938732-70938751 None:intergenic 30.0%
GTACCTACTAGTAATATTCT+TGG + chr4_4:70939343-70939362 Msa0700060:intron 30.0%
AGACCAAGAATATTACTAGT+AGG - chr4_4:70939349-70939368 None:intergenic 30.0%
TGAAAGAATTGCGAAACATA+TGG + chr4_4:70939560-70939579 Msa0700060:CDS 30.0%
GGAATTCACTTAATGAGAAA+TGG + chr4_4:70939637-70939656 Msa0700060:CDS 30.0%
TAAAGAAAGCTAGAGTAGTT+TGG + chr4_4:70939779-70939798 Msa0700060:CDS 30.0%
CTAGATTTATTTAGTGTGCT+TGG - chr4_4:70939916-70939935 None:intergenic 30.0%
CTCTTACACAAAAGATATGA+TGG - chr4_4:70939986-70940005 None:intergenic 30.0%
!! AATTTCTTTTTGGCAGAAGT+TGG + chr4_4:70940023-70940042 Msa0700060:intron 30.0%
!! TCTGATATGTTGTTTCTGTT+AGG + chr4_4:70940177-70940196 Msa0700060:intron 30.0%
! ATTTTGCTCTTCAGAAATAC+CGG + chr4_4:70940346-70940365 Msa0700060:intron 30.0%
!!! GATAGTTTTGGATATTCAGA+TGG + chr4_4:70940510-70940529 Msa0700060:CDS 30.0%
TCTGAACTCTTTCTTATCTT+TGG - chr4_4:70940606-70940625 None:intergenic 30.0%
AAAGATAAGAAAGAGTTCAG+AGG + chr4_4:70940605-70940624 Msa0700060:CDS 30.0%
GAACACACTTGTACAATTAA+AGG + chr4_4:70940740-70940759 Msa0700060:CDS 30.0%
GTGTAACATTTAATGGCAAA+TGG - chr4_4:70940776-70940795 None:intergenic 30.0%
TGTTGCTGTGTAACATTTAA+TGG - chr4_4:70940783-70940802 None:intergenic 30.0%
! TTGAAAGTGATTGGTCAATT+TGG - chr4_4:70940809-70940828 None:intergenic 30.0%
TAATCATTCTTGTAATCGGA+GGG - chr4_4:70940843-70940862 None:intergenic 30.0%
ATGCAACCAAGATTAAATCT+TGG - chr4_4:70940987-70941006 None:intergenic 30.0%
TCTTGGTTGCATTTATGATA+TGG + chr4_4:70940995-70941014 Msa0700060:CDS 30.0%
! CTACCAAAGAAACTTACTTT+TGG + chr4_4:70941022-70941041 Msa0700060:CDS 30.0%
TACCCAAAAGTAAGTTTCTT+TGG - chr4_4:70941028-70941047 None:intergenic 30.0%
CATATTCACCTTGTAACAAA+AGG - chr4_4:70941091-70941110 None:intergenic 30.0%
! TCACCAATCCACATTTTTGT+TGG + chr4_4:70938721-70938740 Msa0700060:intron 35.0%
AAGCCAACAAAAATGTGGAT+TGG - chr4_4:70938727-70938746 None:intergenic 35.0%
CTAGGATGAGATGATAATAC+TGG + chr4_4:70938840-70938859 Msa0700060:intron 35.0%
! TACGACTCATGTGTCTTATT+TGG + chr4_4:70938937-70938956 Msa0700060:intron 35.0%
CATATGCATCTTTACTTTCG+CGG - chr4_4:70939153-70939172 None:intergenic 35.0%
TTCAAGCTTCTTGAACATGT+TGG + chr4_4:70939214-70939233 Msa0700060:CDS 35.0%
!! TATTACTAGTAGGTACTGAC+TGG - chr4_4:70939339-70939358 None:intergenic 35.0%
CGCAATTCTTTCATCCTTAT+AGG - chr4_4:70939553-70939572 None:intergenic 35.0%
AAGAGAATTTGAAAGTCTCG+AGG + chr4_4:70939615-70939634 Msa0700060:CDS 35.0%
AGAGAATTTGAAAGTCTCGA+GGG + chr4_4:70939616-70939635 Msa0700060:CDS 35.0%
GTTAAAGCTGTGAATCAACT+TGG + chr4_4:70939826-70939845 Msa0700060:CDS 35.0%
TAGATTTGATGAATGTTCCG+TGG + chr4_4:70940060-70940079 Msa0700060:CDS 35.0%
AATGATCAGAAATTGTCCTC+AGG + chr4_4:70940396-70940415 Msa0700060:CDS 35.0%
! TGAATGCTTTATTCTACCTG+AGG - chr4_4:70940415-70940434 None:intergenic 35.0%
GATTTACCTTCAAAAGATCC+AGG + chr4_4:70940435-70940454 Msa0700060:CDS 35.0%
! AAACATCCTGGATCTTTTGA+AGG - chr4_4:70940444-70940463 None:intergenic 35.0%
TTGAGAAAGCTTAAACATCC+TGG - chr4_4:70940456-70940475 None:intergenic 35.0%
ATAAGAAAGAGTTCAGAGGT+AGG + chr4_4:70940609-70940628 Msa0700060:CDS 35.0%
TTACTTCTGATTCCAGAGAT+TGG - chr4_4:70940641-70940660 None:intergenic 35.0%
GTAATCATTCTTGTAATCGG+AGG - chr4_4:70940844-70940863 None:intergenic 35.0%
CATTAGTCCTGTGAATTCAT+CGG + chr4_4:70940863-70940882 Msa0700060:CDS 35.0%
!! ATCATTTGAGATGAGGCTTT+TGG - chr4_4:70940919-70940938 None:intergenic 35.0%
! GAGATTGATCATTTGAGATG+AGG - chr4_4:70940926-70940945 None:intergenic 35.0%
! TGTCAGAGTATTATGATCCA+AGG + chr4_4:70941141-70941160 Msa0700060:CDS 35.0%
ATCTGCTGAAGCATACAAAT+TGG - chr4_4:70941186-70941205 None:intergenic 35.0%
GTCTCTTGTCTATGACAACA+TGG + chr4_4:70938438-70938457 Msa0700060:CDS 40.0%
CTTCTTCCATGTGCTAAAAG+TGG + chr4_4:70938619-70938638 Msa0700060:intron 40.0%
ACTAACCAATAACGCTAGCT+AGG + chr4_4:70938822-70938841 Msa0700060:intron 40.0%
! TAGTCTTAATAGCTGCGTGA+TGG + chr4_4:70938970-70938989 Msa0700060:intron 40.0%
AGTCTTAATAGCTGCGTGAT+GGG + chr4_4:70938971-70938990 Msa0700060:intron 40.0%
CTCTATTGCTAAGCAACATG+TGG - chr4_4:70939116-70939135 None:intergenic 40.0%
! CACATGTTGCTTAGCAATAG+AGG + chr4_4:70939114-70939133 Msa0700060:CDS 40.0%
TGAACATGTTGGACTTGAGA+TGG + chr4_4:70939225-70939244 Msa0700060:CDS 40.0%
TATGCAGTGATGTCTGTTGA+TGG + chr4_4:70939469-70939488 Msa0700060:intron 40.0%
! TGTTGATGGAGAAACAAGCA+GGG + chr4_4:70939483-70939502 Msa0700060:CDS 40.0%
!! GTGATGAAAGGTGTTCAACA+TGG + chr4_4:70939505-70939524 Msa0700060:CDS 40.0%
!! GATGGTAACATGTTTGCTGT+AGG + chr4_4:70939676-70939695 Msa0700060:CDS 40.0%
AAGTTCGATCTACGAGTTCT+TGG - chr4_4:70939756-70939775 None:intergenic 40.0%
! ACATTTTCTCTAGTCAGCCA+CGG - chr4_4:70940080-70940099 None:intergenic 40.0%
AAATGTTGCTAGTCACTTGC+AGG + chr4_4:70940094-70940113 Msa0700060:CDS 40.0%
CTAGAACTAGAAGGCTAGTA+GGG + chr4_4:70940201-70940220 Msa0700060:intron 40.0%
TAGAACTAGAAGGCTAGTAG+GGG + chr4_4:70940202-70940221 Msa0700060:intron 40.0%
! AATACCGGCTATACTTGAGT+AGG + chr4_4:70940361-70940380 Msa0700060:CDS 40.0%
TTCAGCAAGCAACAAAACGA+AGG + chr4_4:70940480-70940499 Msa0700060:CDS 40.0%
!! GAAGGTGCAATCGATAGTTT+TGG + chr4_4:70940498-70940517 Msa0700060:CDS 40.0%
!! TCATTCCAAGAGTACTGTGT+TGG - chr4_4:70940690-70940709 None:intergenic 40.0%
ATTCTTGTAATCGGAGGGAT+TGG - chr4_4:70940838-70940857 None:intergenic 40.0%
AAGTTTAAGTCCTCGTCCAA+AGG - chr4_4:70941068-70941087 None:intergenic 40.0%
GGAACTTCAGCAATAAACCT+TGG - chr4_4:70941161-70941180 None:intergenic 40.0%
GCTGAAGCATACAAATTGGT+AGG - chr4_4:70941182-70941201 None:intergenic 40.0%
! AAAGCATCAAGGTGTGGAGA+AGG - chr4_4:70938478-70938497 None:intergenic 45.0%
CTCATCCTAGCTAGCGTTAT+TGG - chr4_4:70938830-70938849 None:intergenic 45.0%
CGATGTAAACATGCCTGACA+TGG + chr4_4:70939186-70939205 Msa0700060:CDS 45.0%
GTAAACATGCCTGACATGGA+TGG + chr4_4:70939190-70939209 Msa0700060:CDS 45.0%
AGCTTGAAACCATCCATGTC+AGG - chr4_4:70939202-70939221 None:intergenic 45.0%
! CTGTTGATGGAGAAACAAGC+AGG + chr4_4:70939482-70939501 Msa0700060:CDS 45.0%
GAAACAAGCAGGGTGATGAA+AGG + chr4_4:70939493-70939512 Msa0700060:CDS 45.0%
GGATCAGATCACACTGATGA+TGG + chr4_4:70939658-70939677 Msa0700060:CDS 45.0%
AGCTAGAGTAGTTTGGTCTG+TGG + chr4_4:70939786-70939805 Msa0700060:CDS 45.0%
CTGTTAGGCCTAGAACTAGA+AGG + chr4_4:70940192-70940211 Msa0700060:intron 45.0%
!! CCTACTAGCCTTCTAGTTCT+AGG - chr4_4:70940203-70940222 None:intergenic 45.0%
CCTAGAACTAGAAGGCTAGT+AGG + chr4_4:70940200-70940219 Msa0700060:intron 45.0%
GCAACCTACTCAAGTATAGC+CGG - chr4_4:70940368-70940387 None:intergenic 45.0%
GGTAGGTCTTAACCAATCTC+TGG + chr4_4:70940626-70940645 Msa0700060:CDS 45.0%
TGGTGGAGTCAAATGCTGTA+TGG - chr4_4:70940665-70940684 None:intergenic 45.0%
!! AGAGTACTGTGTTGGTATGG+TGG - chr4_4:70940682-70940701 None:intergenic 45.0%
!! CCAAGAGTACTGTGTTGGTA+TGG - chr4_4:70940685-70940704 None:intergenic 45.0%
CCATACCAACACAGTACTCT+TGG + chr4_4:70940682-70940701 Msa0700060:CDS 45.0%
GTGTTCTGTTCTTCTTCGAG+TGG - chr4_4:70940726-70940745 None:intergenic 45.0%
GAAGACACCGATGAATTCAC+AGG - chr4_4:70940873-70940892 None:intergenic 45.0%
! GCTTTTGGACTGACTAGGAA+AGG - chr4_4:70940904-70940923 None:intergenic 45.0%
! ATGAGGCTTTTGGACTGACT+AGG - chr4_4:70940909-70940928 None:intergenic 45.0%
!! GCTGGAAAAGCATCAAGGTG+TGG - chr4_4:70938484-70938503 None:intergenic 50.0%
! CACCTTGATGCTTTTCCAGC+TGG + chr4_4:70938484-70938503 Msa0700060:CDS 50.0%
!! GACCAGCTGGAAAAGCATCA+AGG - chr4_4:70938489-70938508 None:intergenic 50.0%
!!! CCTCTCCCAGCTCTTTTTTC+CGG + chr4_4:70939312-70939331 Msa0700060:intron 50.0%
CTGGTCCGGAAAAAAGAGCT+GGG - chr4_4:70939320-70939339 None:intergenic 50.0%
ACTGGTCCGGAAAAAAGAGC+TGG - chr4_4:70939321-70939340 None:intergenic 50.0%
!! CTAGTAGGTACTGACTGGTC+CGG - chr4_4:70939334-70939353 None:intergenic 50.0%
TGAGTCAGCTTCCACACCTT+TGG + chr4_4:70941049-70941068 Msa0700060:CDS 50.0%
TAAGTCCTCGTCCAAAGGTG+TGG - chr4_4:70941063-70941082 None:intergenic 50.0%
ACGAGAACTCGTAGACCAGC+TGG - chr4_4:70938502-70938521 None:intergenic 55.0%
CCGGAAAAAAGAGCTGGGAG+AGG - chr4_4:70939315-70939334 None:intergenic 55.0%
AGCTTCCACACCTTTGGACG+AGG + chr4_4:70941055-70941074 Msa0700060:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 70938424 70941214 70938424 ID=Msa0700060;Name=Msa0700060
chr4_4 mRNA 70938424 70941214 70938424 ID=Msa0700060-mRNA-1;Parent=Msa0700060;Name=Msa0700060-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|5|0|542
chr4_4 exon 70938424 70938586 70938424 ID=Msa0700060-mRNA-1:exon:21513;Parent=Msa0700060-mRNA-1
chr4_4 exon 70939110 70939262 70939110 ID=Msa0700060-mRNA-1:exon:21514;Parent=Msa0700060-mRNA-1
chr4_4 exon 70939476 70939856 70939476 ID=Msa0700060-mRNA-1:exon:21515;Parent=Msa0700060-mRNA-1
chr4_4 exon 70940039 70940115 70940039 ID=Msa0700060-mRNA-1:exon:21516;Parent=Msa0700060-mRNA-1
chr4_4 exon 70940360 70941214 70940360 ID=Msa0700060-mRNA-1:exon:21517;Parent=Msa0700060-mRNA-1
chr4_4 CDS 70938424 70938586 70938424 ID=Msa0700060-mRNA-1:cds;Parent=Msa0700060-mRNA-1
chr4_4 CDS 70939110 70939262 70939110 ID=Msa0700060-mRNA-1:cds;Parent=Msa0700060-mRNA-1
chr4_4 CDS 70939476 70939856 70939476 ID=Msa0700060-mRNA-1:cds;Parent=Msa0700060-mRNA-1
chr4_4 CDS 70940039 70940115 70940039 ID=Msa0700060-mRNA-1:cds;Parent=Msa0700060-mRNA-1
chr4_4 CDS 70940360 70941214 70940360 ID=Msa0700060-mRNA-1:cds;Parent=Msa0700060-mRNA-1
Gene Sequence

>Msa0700060

ATGGTACACAACAGGTCTCTTGTCTATGACAACATGGATAATTCTTGCTTTCCTTCTCCACACCTTGATGCTTTTCCAGCTGGTCTACGAGTTCTCGTTGTTGATGATGATCTAACTTGGCTCAGAATCCTTGAAAAAATGCTAAAAAAATGCTTTTACCGAGTGACCACATGTTGCTTAGCAATAGAGGCTCTTAAGAAGCTCCGCGAAAGTAAAGATGCATATGACATAGTGATCAGCGATGTAAACATGCCTGACATGGATGGTTTCAAGCTTCTTGAACATGTTGGACTTGAGATGGATCTTCCTGTCATTATGATGTCTGTTGATGGAGAAACAAGCAGGGTGATGAAAGGTGTTCAACATGGAGCATGTGATTATTTGCTTAAGCCTATAAGGATGAAAGAATTGCGAAACATATGGCAACATGTCTTAAGAAAAAGAATACATGAGGCAAGAGAATTTGAAAGTCTCGAGGGAATTCACTTAATGAGAAATGGATCAGATCACACTGATGATGGTAACATGTTTGCTGTAGGAAAAGAAATGAACTTAATGAAGAAAAGGAAAAATTTCGATAGTAAACATGATGACCAAGAACTCGTAGATCGAACTTTCATAAAGAAAGCTAGAGTAGTTTGGTCTGTGGATCTTCATCAGAAGTTTGTTAAAGCTGTGAATCAACTTGGATTCGATAAAGTTGGTCCCAAGAAAATTCTAGATTTGATGAATGTTCCGTGGCTGACTAGAGAAAATGTTGCTAGTCACTTGCAGAAATACCGGCTATACTTGAGTAGGTTGCAAAAGAAAAATGATCAGAAATTGTCCTCAGGTAGAATAAAGCATTCAGATTTACCTTCAAAAGATCCAGGATGTTTAAGCTTTCTCAACTCTTTCAGCAAGCAACAAAACGAAGGTGCAATCGATAGTTTTGGATATTCAGATGGGACATTAAAAATTCAAATGGATACTACAGGTCATGAAAAAAGAAGAGCTTCAACTAGTGAAATTTCTGATCCAAAGATAAGAAAGAGTTCAGAGGTAGGTCTTAACCAATCTCTGGAATCAGAAGTAAACCATACAGCATTTGACTCCACCATACCAACACAGTACTCTTGGAATGAAGTTTCTAAAACGCCACTCGAAGAAGAACAGAACACACTTGTACAATTAAAGGATAGCTTCAGCCATTTGCCATTAAATGTTACACAGCAACACTTCCAAATTGACCAATCACTTTCAATTAATTCCAATCCCTCCGATTACAAGAATGATTACATTAGTCCTGTGAATTCATCGGTGTCTTCAGTTGATACCTTTCCTAGTCAGTCCAAAAGCCTCATCTCAAATGATCAATCTCCTGATATCATTTTAACTTCAAATTTGAGTTCAAAAACACCAAGATTTAATCTTGGTTGCATTTATGATATGGAAATCTACCAAAGAAACTTACTTTTGGGTATTGAGTCAGCTTCCACACCTTTGGACGAGGACTTAAACTTCCTTTTGTTACAAGGTGAATATGATATGAACTTTGACATGCAGAATATAGAGATGTCAGAGTATTATGATCCAAGGTTTATTGCTGAAGTTCCTACCAATTTGTATGCTTCAGCAGATTATTCTTAG
Protein sequence

>Msa0700060

MVHNRSLVYDNMDNSCFPSPHLDAFPAGLRVLVVDDDLTWLRILEKMLKKCFYRVTTCCLAIEALKKLRESKDAYDIVISDVNMPDMDGFKLLEHVGLEMDLPVIMMSVDGETSRVMKGVQHGACDYLLKPIRMKELRNIWQHVLRKRIHEAREFESLEGIHLMRNGSDHTDDGNMFAVGKEMNLMKKRKNFDSKHDDQELVDRTFIKKARVVWSVDLHQKFVKAVNQLGFDKVGPKKILDLMNVPWLTRENVASHLQKYRLYLSRLQKKNDQKLSSGRIKHSDLPSKDPGCLSFLNSFSKQQNEGAIDSFGYSDGTLKIQMDTTGHEKRRASTSEISDPKIRKSSEVGLNQSLESEVNHTAFDSTIPTQYSWNEVSKTPLEEEQNTLVQLKDSFSHLPLNVTQQHFQIDQSLSINSNPSDYKNDYISPVNSSVSSVDTFPSQSKSLISNDQSPDIILTSNLSSKTPRFNLGCIYDMEIYQRNLLLGIESASTPLDEDLNFLLLQGEYDMNFDMQNIEMSEYYDPRFIAEVPTNLYASADYS*