AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0661140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0661140 MtrunA17_Chr1g0150151 73.551 276 49 2 1 252 86 361 3.12e-137 390
Msa0661140 MtrunA17_Chr7g0227921 42.606 284 134 5 1 260 85 363 6.46e-73 227
Msa0661140 MtrunA17_Chr6g0451201 39.929 283 141 4 1 260 84 360 1.28e-60 195
Msa0661140 MtrunA17_Chr6g0451211 38.112 286 139 7 1 260 88 361 1.69e-58 190
Msa0661140 MtrunA17_Chr6g0451171 39.223 283 143 5 1 260 86 362 3.98e-58 189
Msa0661140 MtrunA17_Chr3g0126101 36.918 279 136 4 1 252 79 344 1.35e-55 182
Msa0661140 MtrunA17_Chr6g0451251 37.809 283 148 4 1 260 87 364 1.87e-54 179
Msa0661140 MtrunA17_Chr4g0059311 37.906 277 128 6 4 248 89 353 4.18e-53 176
Msa0661140 MtrunA17_Chr5g0413961 36.525 282 126 5 1 247 87 350 7.73e-52 172
Msa0661140 MtrunA17_Chr5g0413921 38.367 245 121 4 6 224 86 326 4.79e-51 170
Msa0661140 MtrunA17_Chr5g0413941 38.655 238 122 4 12 226 92 328 6.48e-50 167
Msa0661140 MtrunA17_Chr6g0451191 37.860 243 118 5 41 258 1 235 1.25e-49 163
Msa0661140 MtrunA17_Chr5g0413931 36.029 272 138 6 6 250 85 347 8.12e-49 164
Msa0661140 MtrunA17_Chr5g0413951 37.288 236 124 4 12 224 92 326 1.37e-48 164
Msa0661140 MtrunA17_Chr6g0451261 40.175 229 109 4 1 204 85 310 2.18e-48 171
Msa0661140 MtrunA17_Chr7g0255561 31.727 249 144 2 3 225 90 338 7.80e-40 142
Msa0661140 MtrunA17_Chr7g0255581 31.727 249 144 2 3 225 90 338 7.80e-40 142
Msa0661140 MtrunA17_Chr7g0271561 35.271 258 130 7 6 230 86 339 2.46e-38 137
Msa0661140 MtrunA17_Chr8g0354121 30.314 287 161 6 3 258 89 367 1.05e-37 136
Msa0661140 MtrunA17_Chr1g0184221 33.333 246 133 6 10 229 113 353 9.75e-37 133
Msa0661140 MtrunA17_Chr1g0160111 31.955 266 149 5 5 238 85 350 1.16e-36 133
Msa0661140 MtrunA17_Chr3g0114731 31.600 250 143 4 3 228 94 339 1.30e-36 133
Msa0661140 MtrunA17_Chr6g0483401 32.258 248 132 4 12 228 95 337 3.23e-35 129
Msa0661140 MtrunA17_Chr1g0155761 29.054 296 160 6 12 259 106 399 1.05e-34 128
Msa0661140 MtrunA17_Chr3g0081511 30.081 246 148 2 3 224 93 338 1.51e-34 128
Msa0661140 MtrunA17_Chr1g0150651 76.829 82 18 1 41 121 1 82 5.35e-34 118
Msa0661140 MtrunA17_Chr2g0278561 29.577 284 173 4 2 260 90 371 3.00e-33 124
Msa0661140 MtrunA17_Chr1g0186561 34.274 248 128 6 10 230 99 338 4.04e-33 123
Msa0661140 MtrunA17_Chr1g0186611 31.984 247 136 5 10 231 99 338 9.00e-33 122
Msa0661140 MtrunA17_Chr3g0126091 29.478 268 164 3 4 248 91 356 1.40e-32 122
Msa0661140 MtrunA17_Chr1g0164831 31.513 238 140 1 12 226 86 323 1.81e-32 121
Msa0661140 MtrunA17_Chr2g0278541 27.891 294 161 7 10 259 95 381 4.96e-32 121
Msa0661140 MtrunA17_Chr1g0182291 28.772 285 158 6 3 261 91 356 5.34e-32 120
Msa0661140 MtrunA17_Chr1g0160171 32.353 238 137 4 12 226 101 337 1.24e-31 120
Msa0661140 MtrunA17_Chr3g0114691 28.731 268 163 4 12 252 105 371 3.00e-31 119
Msa0661140 MtrunA17_Chr1g0186571 32.271 251 130 7 10 231 96 335 3.32e-31 118
Msa0661140 MtrunA17_Chr2g0284261 27.413 259 162 2 12 247 93 348 5.01e-31 118
Msa0661140 MtrunA17_Chr3g0081421 29.084 251 145 4 3 223 93 340 7.91e-31 118
Msa0661140 MtrunA17_Chr3g0142131 29.391 279 148 6 12 252 105 372 9.18e-31 117
Msa0661140 MtrunA17_Chr7g0263101 28.873 284 157 7 3 255 89 358 1.28e-30 117
Msa0661140 MtrunA17_Chr3g0114711 26.780 295 153 5 3 238 90 380 1.30e-30 117
Msa0661140 MtrunA17_Chr4g0073241 29.371 286 155 6 1 249 84 359 1.63e-30 117
Msa0661140 MtrunA17_Chr1g0160181 28.175 252 146 3 3 224 94 340 4.88e-30 115
Msa0661140 MtrunA17_Chr2g0310441 26.740 273 168 5 12 261 97 360 1.11e-29 114
Msa0661140 MtrunA17_Chr1g0171301 30.645 248 138 6 10 230 99 339 1.76e-29 114
Msa0661140 MtrunA17_Chr3g0129591 27.174 276 163 3 12 261 92 355 3.15e-29 113
Msa0661140 MtrunA17_Chr1g0186601 31.984 247 134 7 10 230 99 337 3.38e-29 113
Msa0661140 MtrunA17_Chr2g0278551 28.364 275 170 5 10 260 95 366 3.74e-29 113
Msa0661140 MtrunA17_Chr8g0334761 27.076 277 168 5 10 253 94 369 3.79e-29 113
Msa0661140 MtrunA17_Chr1g0186641 28.452 239 144 2 10 224 96 331 9.07e-29 111
Msa0661140 MtrunA17_Chr3g0129571 28.571 238 144 2 12 223 94 331 1.21e-28 111
Msa0661140 MtrunA17_Chr4g0051021 27.599 279 170 4 2 255 89 360 8.68e-28 109
Msa0661140 MtrunA17_Chr3g0129601 28.163 245 143 4 12 227 114 354 9.18e-28 109
Msa0661140 MtrunA17_Chr2g0290611 28.674 279 162 4 10 258 95 366 1.07e-27 109
Msa0661140 MtrunA17_Chr5g0407971 27.046 281 161 5 6 248 90 364 4.28e-26 105
Msa0661140 MtrunA17_Chr6g0463971 28.800 250 146 5 10 228 98 346 1.12e-25 103
Msa0661140 MtrunA17_Chr4g0050891 28.216 241 132 4 12 218 98 331 4.12e-25 102
Msa0661140 MtrunA17_Chr4g0050881 25.969 258 152 3 2 227 83 333 5.50e-25 101
Msa0661140 MtrunA17_Chr5g0408001 28.235 255 147 3 6 224 89 343 3.50e-24 100
Msa0661140 MtrunA17_Chr6g0451241 47.475 99 50 1 162 260 18 114 1.04e-23 92.4
Msa0661140 MtrunA17_Chr8g0369841 26.908 249 146 3 12 224 94 342 1.06e-23 98.6
Msa0661140 MtrunA17_Chr8g0383731 27.313 227 137 3 26 224 104 330 1.28e-23 97.8
Msa0661140 MtrunA17_Chr4g0050941 26.335 281 165 5 12 250 98 378 1.59e-23 98.2
Msa0661140 MtrunA17_Chr2g0332001 27.715 267 163 5 19 258 130 393 3.08e-23 97.4
Msa0661140 MtrunA17_Chr3g0084091 28.631 241 141 3 10 223 13 249 5.92e-23 95.1
Msa0661140 MtrunA17_Chr5g0441761 28.151 238 140 5 10 223 97 327 8.92e-23 95.5
Msa0661140 MtrunA17_Chr3g0129581 25.725 276 167 3 12 261 92 355 1.43e-22 95.1
Msa0661140 MtrunA17_Chr5g0407991 27.344 256 150 4 6 225 88 343 1.67e-22 95.1
Msa0661140 MtrunA17_Chr3g0114701 25.725 276 163 5 3 248 85 348 3.42e-22 94.4
Msa0661140 MtrunA17_Chr5g0404811 29.185 233 141 3 40 248 6 238 6.36e-22 91.3
Msa0661140 MtrunA17_Chr5g0408041 25.606 289 174 5 5 255 89 374 8.31e-22 93.2
Msa0661140 MtrunA17_Chr8g0334751 27.925 265 165 4 10 249 95 358 8.68e-22 92.8
Msa0661140 MtrunA17_Chr5g0429001 26.027 292 174 4 10 259 34 325 1.80e-21 91.7
Msa0661140 MtrunA17_Chr5g0408031 27.969 261 146 5 5 225 88 346 3.71e-21 91.7
Msa0661140 MtrunA17_Chr8g0334771 27.826 230 140 3 26 230 110 338 4.38e-21 91.3
Msa0661140 MtrunA17_Chr4g0050931 26.271 236 144 5 31 237 77 311 7.90e-21 89.7
Msa0661140 MtrunA17_Chr2g0278021 26.613 248 143 3 41 249 1 248 1.26e-20 88.6
Msa0661140 MtrunA17_Chr5g0428881 27.197 239 149 3 10 223 101 339 1.56e-20 89.7
Msa0661140 MtrunA17_Chr1g0177221 31.724 145 93 2 4 146 95 235 4.37e-20 87.0
Msa0661140 MtrunA17_Chr5g0399281 26.236 263 157 5 2 230 83 342 7.42e-20 87.4
Msa0661140 MtrunA17_Chr1g0211801 35.000 120 78 0 12 131 94 213 2.11e-19 85.1
Msa0661140 MtrunA17_Chr4g0056081 25.200 250 147 6 11 227 99 341 4.59e-18 82.4
Msa0661140 MtrunA17_Chr1g0150641 80.435 46 9 0 1 46 13 58 1.80e-17 74.3
Msa0661140 MtrunA17_Chr4g0051001 25.333 225 143 2 2 201 106 330 4.98e-16 76.6
Msa0661140 MtrunA17_Chr8g0334711 32.773 119 79 1 3 121 86 203 1.92e-15 73.2
Msa0661140 MtrunA17_Chr6g0451231 37.500 80 46 1 41 120 1 76 1.48e-13 65.1
Msa0661140 MtrunA17_Chr8g0334731 30.702 114 76 2 10 121 75 187 3.48e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0661140 AT5G40230.1 50.000 250 100 2 1 225 94 343 1.37e-81 249
Msa0661140 AT5G40240.1 45.588 272 121 3 1 247 93 362 2.76e-81 249
Msa0661140 AT5G40240.3 45.588 272 121 3 1 247 93 362 2.76e-81 249
Msa0661140 AT5G40240.2 45.588 272 121 3 1 247 107 376 7.00e-81 248
Msa0661140 AT4G15540.1 49.600 250 92 2 1 227 90 328 6.12e-72 224
Msa0661140 AT4G15540.2 49.600 250 92 2 1 227 90 328 6.12e-72 224
Msa0661140 AT3G28050.1 41.844 282 123 7 1 247 85 360 5.45e-69 217
Msa0661140 AT5G40210.1 40.511 274 116 5 1 247 83 336 6.46e-65 206
Msa0661140 AT3G28070.2 39.033 269 130 5 7 249 4 264 4.59e-60 191
Msa0661140 AT3G28070.1 38.545 275 135 5 1 249 90 356 3.36e-59 192
Msa0661140 AT3G28130.5 40.959 271 128 5 7 253 6 268 5.94e-59 188
Msa0661140 AT3G28130.1 40.959 271 128 5 7 253 6 268 5.94e-59 188
Msa0661140 AT3G28130.2 41.155 277 131 6 1 253 86 354 2.27e-58 189
Msa0661140 AT3G28100.1 39.194 273 133 5 1 247 87 352 1.62e-56 185
Msa0661140 AT3G28080.5 38.113 265 131 6 7 245 4 261 4.87e-54 176
Msa0661140 AT3G28080.7 38.113 265 131 6 7 245 4 261 4.87e-54 176
Msa0661140 AT3G28080.8 38.113 265 131 6 7 245 4 261 4.87e-54 176
Msa0661140 AT3G28080.6 38.113 265 131 6 7 245 4 261 4.87e-54 176
Msa0661140 AT3G28080.2 37.638 271 136 6 1 245 79 342 1.63e-53 177
Msa0661140 AT3G28080.1 37.638 271 136 6 1 245 87 350 2.83e-53 176
Msa0661140 AT3G28080.4 37.190 242 111 5 7 245 4 207 1.78e-46 154
Msa0661140 AT3G28070.3 37.004 227 115 3 1 201 90 314 4.01e-46 157
Msa0661140 AT3G28080.3 36.694 248 116 5 1 245 87 296 7.95e-46 155
Msa0661140 AT3G28070.5 35.745 235 117 5 41 249 1 227 5.05e-43 146
Msa0661140 AT3G28130.3 37.975 237 115 6 41 253 1 229 6.92e-43 146
Msa0661140 AT3G28080.9 35.498 231 116 6 41 245 1 224 1.10e-38 135
Msa0661140 AT4G08290.1 31.783 258 146 4 3 233 88 342 6.64e-37 134
Msa0661140 AT1G75500.1 29.329 283 155 5 12 249 104 386 1.85e-35 130
Msa0661140 AT1G75500.2 29.329 283 155 5 12 249 104 386 1.85e-35 130
Msa0661140 AT3G30340.1 32.353 238 136 3 12 225 95 331 7.09e-35 129
Msa0661140 AT3G18200.2 32.197 264 145 5 12 249 119 374 9.49e-35 129
Msa0661140 AT2G39510.1 28.772 285 167 5 10 259 90 373 1.89e-34 127
Msa0661140 AT1G70260.1 30.980 255 144 3 6 228 93 347 2.37e-33 125
Msa0661140 AT5G07050.1 31.746 252 140 4 3 223 93 343 3.30e-33 125
Msa0661140 AT2G40900.1 29.317 249 147 3 3 228 86 328 3.97e-33 124
Msa0661140 AT1G44800.1 30.992 242 139 4 10 226 93 331 6.96e-32 120
Msa0661140 AT3G28100.3 42.953 149 80 2 1 146 87 233 7.03e-32 118
Msa0661140 AT1G09380.1 29.151 271 146 4 6 230 86 356 1.33e-31 120
Msa0661140 AT3G28130.4 42.857 147 83 1 1 146 86 232 1.70e-31 117
Msa0661140 AT1G21890.1 28.185 259 155 2 3 230 86 344 2.66e-31 119
Msa0661140 AT1G11450.1 32.283 254 139 7 12 239 8 254 7.48e-31 115
Msa0661140 AT4G08300.1 29.675 246 146 5 10 228 93 338 1.47e-30 117
Msa0661140 AT1G11450.2 32.283 254 139 7 12 239 49 295 2.76e-30 115
Msa0661140 AT1G11450.5 32.283 254 139 7 12 239 56 302 3.36e-30 115
Msa0661140 AT3G18200.1 30.556 252 141 5 24 249 1 244 4.83e-30 113
Msa0661140 AT1G11450.3 32.283 254 139 7 12 239 100 346 5.39e-30 115
Msa0661140 AT5G13670.1 29.960 247 148 3 3 224 83 329 2.02e-29 114
Msa0661140 AT4G01430.1 26.199 271 172 5 12 256 93 361 2.14e-29 114
Msa0661140 AT5G64700.1 29.070 258 154 3 12 240 92 349 8.34e-29 112
Msa0661140 AT1G01070.2 29.237 236 143 3 12 223 53 288 1.64e-28 110
Msa0661140 AT1G01070.1 28.750 240 147 3 12 227 100 339 3.16e-28 110
Msa0661140 AT2G37460.1 27.660 282 171 5 3 257 90 365 5.25e-28 110
Msa0661140 AT1G43650.1 31.518 257 135 6 7 229 78 327 1.33e-27 108
Msa0661140 AT4G30420.1 27.682 289 167 7 11 259 85 371 1.47e-27 109
Msa0661140 AT3G53210.2 29.114 237 145 2 12 225 37 273 1.49e-27 108
Msa0661140 AT4G30420.2 27.682 289 167 7 11 259 95 381 1.64e-27 109
Msa0661140 AT3G28100.2 44.882 127 65 2 1 124 87 211 3.93e-27 105
Msa0661140 AT3G53210.1 29.114 237 145 2 12 225 94 330 4.09e-27 108
Msa0661140 AT3G28130.6 44.800 125 68 1 1 124 86 210 6.31e-27 104
Msa0661140 AT1G11460.1 30.252 238 137 5 12 223 100 334 8.00e-27 106
Msa0661140 AT4G28040.1 25.896 251 151 4 4 231 86 324 8.68e-26 104
Msa0661140 AT4G28040.5 25.896 251 151 4 4 231 86 324 8.68e-26 104
Msa0661140 AT4G28040.3 25.896 251 151 4 4 231 86 324 8.68e-26 104
Msa0661140 AT4G28040.4 25.896 251 151 4 4 231 86 324 8.68e-26 104
Msa0661140 AT4G28040.2 25.896 251 151 4 4 231 86 324 8.68e-26 104
Msa0661140 AT4G01440.1 25.514 243 154 4 12 228 94 335 1.77e-24 100
Msa0661140 AT2G37450.2 27.437 277 166 4 3 255 63 328 2.91e-24 99.8
Msa0661140 AT5G45370.6 28.287 251 144 3 12 226 2 252 6.09e-24 97.8
Msa0661140 AT5G45370.5 28.287 251 144 3 12 226 2 252 6.09e-24 97.8
Msa0661140 AT5G45370.1 28.807 243 141 2 6 226 98 330 7.86e-24 99.0
Msa0661140 AT5G45370.2 28.627 255 146 3 6 224 98 352 8.89e-24 99.0
Msa0661140 AT5G45370.4 28.627 255 146 3 6 224 98 352 8.89e-24 99.0
Msa0661140 AT2G37450.3 27.138 269 157 4 13 255 1 256 1.33e-23 96.7
Msa0661140 AT4G19185.2 26.744 258 149 5 6 225 21 276 1.35e-23 97.8
Msa0661140 AT1G68170.2 25.556 270 159 4 12 242 97 363 2.25e-23 97.8
Msa0661140 AT1G68170.1 25.556 270 159 4 12 242 87 353 2.29e-23 97.8
Msa0661140 AT4G19185.1 26.744 258 149 5 6 225 99 354 2.69e-23 98.2
Msa0661140 AT4G01430.2 27.778 216 130 4 12 201 93 308 1.18e-22 95.1
Msa0661140 AT3G56620.1 26.923 234 143 2 10 220 93 321 1.84e-22 95.5
Msa0661140 AT4G08290.2 36.601 153 90 3 3 151 88 237 8.58e-22 92.0
Msa0661140 AT3G30340.2 37.190 121 74 2 12 131 95 214 1.55e-21 91.3
Msa0661140 AT1G11450.4 31.019 216 119 6 12 201 100 311 2.45e-21 91.7
Msa0661140 AT1G21890.2 26.522 230 138 2 3 201 86 315 6.63e-21 90.9
Msa0661140 AT1G60050.1 26.190 252 157 3 6 228 94 345 1.32e-20 90.1
Msa0661140 AT4G01440.5 24.017 229 147 4 27 229 1 228 1.46e-20 88.2
Msa0661140 AT4G01440.2 24.017 229 147 4 27 229 1 228 1.46e-20 88.2
Msa0661140 AT1G25270.1 25.373 268 174 3 12 254 87 353 3.79e-20 88.6
Msa0661140 AT4G24980.1 25.792 221 133 5 10 226 48 241 4.88e-20 87.0
Msa0661140 AT3G45870.1 26.531 245 148 2 6 218 90 334 5.92e-20 88.6
Msa0661140 AT4G01440.3 24.883 213 136 3 12 201 94 305 6.43e-20 87.8
Msa0661140 AT3G53210.3 35.000 120 78 0 12 131 94 213 2.84e-19 85.1
Msa0661140 AT5G47470.2 24.615 260 161 5 11 245 142 391 9.16e-19 85.1
Msa0661140 AT5G47470.1 24.615 260 161 5 11 245 114 363 1.32e-18 84.3
Msa0661140 AT4G16620.1 23.967 242 156 3 11 227 96 334 3.63e-18 83.2
Msa0661140 AT5G47470.3 23.967 242 152 4 11 227 142 376 4.39e-18 83.2
Msa0661140 AT4G16620.4 23.750 240 155 3 13 227 1 237 5.60e-18 81.3
Msa0661140 AT4G16620.2 23.750 240 155 3 13 227 1 237 5.60e-18 81.3
Msa0661140 AT4G01430.3 23.451 226 146 4 56 256 1 224 6.83e-18 80.5
Msa0661140 AT4G01440.4 30.000 140 93 2 12 147 94 232 2.62e-17 79.7
Msa0661140 AT3G45870.8 26.316 228 136 2 6 201 90 317 1.32e-15 75.5
Msa0661140 AT5G45370.3 26.667 225 127 4 38 224 68 292 6.13e-15 73.6
Msa0661140 AT4G01450.3 31.356 118 76 2 12 129 94 206 3.57e-14 70.5
Msa0661140 AT4G01450.4 31.429 140 87 3 12 147 94 228 5.75e-14 70.1
Msa0661140 AT4G01450.1 31.429 140 87 3 12 147 94 228 7.38e-14 70.9
Msa0661140 AT4G01450.2 31.429 140 87 3 12 147 94 228 9.15e-14 70.5
Msa0661140 AT3G28060.1 33.333 135 55 5 92 201 30 154 3.53e-13 65.9
Msa0661140 AT2G37450.1 23.554 242 150 4 38 255 59 289 1.12e-12 67.0
Msa0661140 AT5G64700.2 28.402 169 114 3 12 174 92 259 2.82e-12 65.5
Msa0661140 AT1G43650.2 35.461 141 75 4 7 137 78 212 5.46e-12 64.7
Msa0661140 AT3G45870.5 23.223 211 130 2 40 218 49 259 1.63e-11 63.5
Msa0661140 AT3G45870.9 29.323 133 84 1 12 134 96 228 3.83e-11 62.4

Find 45 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGTGATGCACTATATTT+TGG 0.111437 4_3:+72360867 Msa0661140:CDS
TACTGTAGAACCCAAAATTT+TGG 0.167195 4_3:-72359988 None:intergenic
CTCTTCATTTGATTCTTCTT+TGG 0.199822 4_3:-72361089 None:intergenic
AAGCAGTCAAGCCAAAATTT+TGG 0.204317 4_3:+72359977 Msa0661140:CDS
GCTCAACATCACAATCAAAT+TGG 0.207645 4_3:+72360108 Msa0661140:CDS
ACTTATAATTGTTCTTTATA+AGG 0.221255 4_3:+72360025 Msa0661140:CDS
TCTTCTAGTGCTATATTTCT+TGG 0.232017 4_3:+72360849 Msa0661140:CDS
TCACAATCAAATTGGGTTCT+TGG 0.268225 4_3:+72360116 Msa0661140:CDS
GGTTCTACAGTATCAATATT+AGG 0.273000 4_3:+72359999 Msa0661140:CDS
CATATTATTGATTCTTCAAT+AGG 0.295205 4_3:+72360086 Msa0661140:CDS
AGTATCTTATTGTGCCAATA+TGG 0.303445 4_3:+72360159 Msa0661140:CDS
ACTTGGGGACTCAATGTTAA+AGG 0.305542 4_3:+72360777 Msa0661140:CDS
CTTATAATTGTTCTTTATAA+GGG 0.309940 4_3:+72360026 Msa0661140:CDS
TGAATGTAAAAGCAGGTATA+AGG 0.350843 4_3:-72359055 None:intergenic
ATCAAATGTTGCAGTGTAAT+CGG 0.355898 4_3:+72361019 Msa0661140:intron
GGTTCTAGTACTAAGCATAT+TGG 0.369589 4_3:-72360378 None:intergenic
CAGTAAAGGAGTCTTAATAT+TGG 0.410828 4_3:-72361140 None:intergenic
AGTAAAGGAGTCTTAATATT+GGG 0.429458 4_3:-72361139 None:intergenic
CTCAACATCACAATCAAATT+GGG 0.447329 4_3:+72360109 Msa0661140:CDS
GCTCAGCTTTGTGGATATAG+AGG 0.487803 4_3:+72358990 Msa0661140:CDS
TAAGTTGTCTTGTTCACACT+TGG 0.500583 4_3:+72360760 Msa0661140:CDS
AATACCACAATAACCTCTGA+TGG 0.504452 4_3:-72360321 None:intergenic
TGTAAAAGCAGGTATAAGGT+TGG 0.506777 4_3:-72359051 None:intergenic
CAATCAAATTGGGTTCTTGG+AGG 0.522223 4_3:+72360119 Msa0661140:CDS
ATGTTGGAGTCCTGCAGTAA+AGG 0.523727 4_3:-72361154 None:intergenic
TATTAAGACTCCTTTACTGC+AGG 0.525189 4_3:+72361144 Msa0661140:CDS
AAGTTGTCTTGTTCACACTT+GGG 0.544449 4_3:+72360761 Msa0661140:CDS
AGTTGTCTTGTTCACACTTG+GGG 0.552518 4_3:+72360762 Msa0661140:CDS
GAAAGAGATGAAGCAAGAGT+AGG 0.563654 4_3:-72359029 None:intergenic
GCAAGGATGAATGTAAAAGC+AGG 0.573221 4_3:-72359062 None:intergenic
CAAAACAATTGTTGAAGATG+TGG 0.576566 4_3:+72361199 Msa0661140:exon
ATTGAAGAATCAATAATATG+TGG 0.584688 4_3:-72360083 None:intergenic
TTAAAGAGTGATATATACAC+AGG 0.588788 4_3:-72360801 None:intergenic
GGAGCAATAATACTATCGGT+TGG 0.591133 4_3:+72361040 Msa0661140:CDS
GCAGCAATTGCTATTGACAA+AGG 0.592127 4_3:-72360825 None:intergenic
AATCGGAGCAATAATACTAT+CGG 0.597408 4_3:+72361036 Msa0661140:CDS
AAAGGAGTCTTAATATTGGG+TGG 0.610630 4_3:-72361136 None:intergenic
GGAGTCTTAATATTGGGTGG+AGG 0.611879 4_3:-72361133 None:intergenic
AGAGTTGTAGCTCAGCTTTG+TGG 0.619119 4_3:+72358981 Msa0661140:intron
AACAAGACAACTTAGTCCTG+TGG 0.621230 4_3:-72360751 None:intergenic
ATATCCATCAGAGGTTATTG+TGG 0.628227 4_3:+72360317 Msa0661140:CDS
TAGACACTAGTGCTCCACAT+AGG 0.657311 4_3:-72360353 None:intergenic
TATCAAACAATATCCATCAG+AGG 0.665266 4_3:+72360308 Msa0661140:CDS
GAAGAATCAATAATATGTGG+TGG 0.696723 4_3:-72360080 None:intergenic
TAAAGAGTGATATATACACA+GGG 0.734244 4_3:-72360800 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTTAAAAAAAAATTA+GGG - chr4_3:72360911-72360930 None:intergenic 0.0%
!!! AAAATTTTAAAAAAAAATTA+GGG - chr4_3:72360911-72360930 None:intergenic 0.0%
!! TATATTAATATATGATGTTT+AGG + chr4_3:72359354-72359373 Msa0661140:intron 10.0%
!! TATATAATAATAAAAAGGTA+CGG - chr4_3:72359466-72359485 None:intergenic 10.0%
!! ATACTATAATACATAATTAA+AGG + chr4_3:72359497-72359516 Msa0661140:intron 10.0%
!!! TTTTTTTTTAGAACATTAAA+CGG + chr4_3:72359698-72359717 Msa0661140:intron 10.0%
!! AAAAATGATAAAAATAAAAG+TGG - chr4_3:72359730-72359749 None:intergenic 10.0%
!!! ACTTTTATTTTTATCATTTT+TGG + chr4_3:72359729-72359748 Msa0661140:intron 10.0%
!! TATATTAATATATGATGTTT+AGG + chr4_3:72359354-72359373 Msa0661140:intron 10.0%
!! TATATAATAATAAAAAGGTA+CGG - chr4_3:72359466-72359485 None:intergenic 10.0%
!! ATACTATAATACATAATTAA+AGG + chr4_3:72359497-72359516 Msa0661140:intron 10.0%
!!! TTTTTTTTTAGAACATTAAA+CGG + chr4_3:72359698-72359717 Msa0661140:intron 10.0%
!! AAAAATGATAAAAATAAAAG+TGG - chr4_3:72359730-72359749 None:intergenic 10.0%
!!! ACTTTTATTTTTATCATTTT+TGG + chr4_3:72359729-72359748 Msa0661140:intron 10.0%
!!! GAAAATAAAACAGTTTTTTT+TGG - chr4_3:72359630-72359649 None:intergenic 15.0%
!!! AAAAAACTGTTTTATTTTCT+TGG + chr4_3:72359631-72359650 Msa0661140:intron 15.0%
!!! TTTTTTTTAGAACATTAAAC+GGG + chr4_3:72359699-72359718 Msa0661140:intron 15.0%
!! ACACACAAAAAATATAAAAA+TGG - chr4_3:72359867-72359886 None:intergenic 15.0%
!! TTCATATTATAATTACTTGT+TGG + chr4_3:72359887-72359906 Msa0661140:intron 15.0%
!!! TCATTTTGAACATTATATAT+TGG + chr4_3:72359914-72359933 Msa0661140:intron 15.0%
!! ACTTATAATTGTTCTTTATA+AGG + chr4_3:72360025-72360044 Msa0661140:CDS 15.0%
!! CTTATAATTGTTCTTTATAA+GGG + chr4_3:72360026-72360045 Msa0661140:CDS 15.0%
!! ATGTATCTATCATAAATAAT+AGG + chr4_3:72360548-72360567 Msa0661140:intron 15.0%
!!! GAAAATAAAACAGTTTTTTT+TGG - chr4_3:72359630-72359649 None:intergenic 15.0%
!!! AAAAAACTGTTTTATTTTCT+TGG + chr4_3:72359631-72359650 Msa0661140:intron 15.0%
!!! TTTTTTTTAGAACATTAAAC+GGG + chr4_3:72359699-72359718 Msa0661140:intron 15.0%
!! ACACACAAAAAATATAAAAA+TGG - chr4_3:72359867-72359886 None:intergenic 15.0%
!! TTCATATTATAATTACTTGT+TGG + chr4_3:72359887-72359906 Msa0661140:intron 15.0%
!!! TCATTTTGAACATTATATAT+TGG + chr4_3:72359914-72359933 Msa0661140:intron 15.0%
!! ACTTATAATTGTTCTTTATA+AGG + chr4_3:72360025-72360044 Msa0661140:CDS 15.0%
!! CTTATAATTGTTCTTTATAA+GGG + chr4_3:72360026-72360045 Msa0661140:CDS 15.0%
!! ATGTATCTATCATAAATAAT+AGG + chr4_3:72360548-72360567 Msa0661140:intron 15.0%
!! CAATATTATATTAGTTACGA+TGG + chr4_3:72359294-72359313 Msa0661140:intron 20.0%
!! ATATATACTCCTTTAACAAA+TGG - chr4_3:72359339-72359358 None:intergenic 20.0%
!! CACAAAAAATATAAAAATGG+TGG - chr4_3:72359864-72359883 None:intergenic 20.0%
!! ATTGAAGAATCAATAATATG+TGG - chr4_3:72360086-72360105 None:intergenic 20.0%
!!! CATATTATTGATTCTTCAAT+AGG + chr4_3:72360086-72360105 Msa0661140:CDS 20.0%
!! TTATGTTAGTGTCTTATATA+TGG - chr4_3:72360259-72360278 None:intergenic 20.0%
!! TCCTATTTAAAAAAAGAGTT+GGG - chr4_3:72360602-72360621 None:intergenic 20.0%
!!! TTTTTCTCTATCTATTATGT+TGG - chr4_3:72361173-72361192 None:intergenic 20.0%
!! CAATATTATATTAGTTACGA+TGG + chr4_3:72359294-72359313 Msa0661140:intron 20.0%
!! ATATATACTCCTTTAACAAA+TGG - chr4_3:72359339-72359358 None:intergenic 20.0%
!! CACAAAAAATATAAAAATGG+TGG - chr4_3:72359864-72359883 None:intergenic 20.0%
!! ATTGAAGAATCAATAATATG+TGG - chr4_3:72360086-72360105 None:intergenic 20.0%
!!! CATATTATTGATTCTTCAAT+AGG + chr4_3:72360086-72360105 Msa0661140:CDS 20.0%
!! TTATGTTAGTGTCTTATATA+TGG - chr4_3:72360259-72360278 None:intergenic 20.0%
!! TCCTATTTAAAAAAAGAGTT+GGG - chr4_3:72360602-72360621 None:intergenic 20.0%
!!! TTTTTCTCTATCTATTATGT+TGG - chr4_3:72361173-72361192 None:intergenic 20.0%
!! GCTTGAAGTATTAACAAATA+AGG + chr4_3:72359144-72359163 Msa0661140:intron 25.0%
! ATAATACATAATTAAAGGCC+TGG + chr4_3:72359502-72359521 Msa0661140:intron 25.0%
!!! GTATTTTTATACAACCTATG+TGG + chr4_3:72360339-72360358 Msa0661140:CDS 25.0%
! TTAGTACTAGAACCAAATTT+GGG + chr4_3:72360387-72360406 Msa0661140:intron 25.0%
! CTTTCTCTTATAAATAGGAT+CGG - chr4_3:72360510-72360529 None:intergenic 25.0%
! TCCTATTTATAAGAGAAAGT+TGG + chr4_3:72360511-72360530 Msa0661140:intron 25.0%
! CCTATTTATAAGAGAAAGTT+GGG + chr4_3:72360512-72360531 Msa0661140:intron 25.0%
! TCTATCATAAATAATAGGAC+TGG + chr4_3:72360553-72360572 Msa0661140:intron 25.0%
!!! ACCCAACTCTTTTTTTAAAT+AGG + chr4_3:72360598-72360617 Msa0661140:intron 25.0%
! GTCCTATTTAAAAAAAGAGT+TGG - chr4_3:72360603-72360622 None:intergenic 25.0%
!!! CTTTTTTTAAATAGGACTGA+AGG + chr4_3:72360606-72360625 Msa0661140:intron 25.0%
!!! AAATTTTTTCACTTTGATGC+AGG + chr4_3:72360719-72360738 Msa0661140:intron 25.0%
!!! AATTTTTTCACTTTGATGCA+GGG + chr4_3:72360720-72360739 Msa0661140:intron 25.0%
! TAAAGAGTGATATATACACA+GGG - chr4_3:72360803-72360822 None:intergenic 25.0%
! TTAAAGAGTGATATATACAC+AGG - chr4_3:72360804-72360823 None:intergenic 25.0%
!!! TTGGTTTTTATGGTGTTATA+TGG + chr4_3:72361059-72361078 Msa0661140:CDS 25.0%
!!! TGGTTTTTATGGTGTTATAT+GGG + chr4_3:72361060-72361079 Msa0661140:CDS 25.0%
! AGTAAAGGAGTCTTAATATT+GGG - chr4_3:72361142-72361161 None:intergenic 25.0%
!! GCTTGAAGTATTAACAAATA+AGG + chr4_3:72359144-72359163 Msa0661140:intron 25.0%
! ATAATACATAATTAAAGGCC+TGG + chr4_3:72359502-72359521 Msa0661140:intron 25.0%
!!! GTATTTTTATACAACCTATG+TGG + chr4_3:72360339-72360358 Msa0661140:CDS 25.0%
! TTAGTACTAGAACCAAATTT+GGG + chr4_3:72360387-72360406 Msa0661140:intron 25.0%
! CTTTCTCTTATAAATAGGAT+CGG - chr4_3:72360510-72360529 None:intergenic 25.0%
! TCCTATTTATAAGAGAAAGT+TGG + chr4_3:72360511-72360530 Msa0661140:intron 25.0%
! CCTATTTATAAGAGAAAGTT+GGG + chr4_3:72360512-72360531 Msa0661140:intron 25.0%
! TCTATCATAAATAATAGGAC+TGG + chr4_3:72360553-72360572 Msa0661140:intron 25.0%
!!! ACCCAACTCTTTTTTTAAAT+AGG + chr4_3:72360598-72360617 Msa0661140:intron 25.0%
! GTCCTATTTAAAAAAAGAGT+TGG - chr4_3:72360603-72360622 None:intergenic 25.0%
!!! CTTTTTTTAAATAGGACTGA+AGG + chr4_3:72360606-72360625 Msa0661140:intron 25.0%
!!! AAATTTTTTCACTTTGATGC+AGG + chr4_3:72360719-72360738 Msa0661140:intron 25.0%
!!! AATTTTTTCACTTTGATGCA+GGG + chr4_3:72360720-72360739 Msa0661140:intron 25.0%
! TAAAGAGTGATATATACACA+GGG - chr4_3:72360803-72360822 None:intergenic 25.0%
! TTAAAGAGTGATATATACAC+AGG - chr4_3:72360804-72360823 None:intergenic 25.0%
!!! TTGGTTTTTATGGTGTTATA+TGG + chr4_3:72361059-72361078 Msa0661140:CDS 25.0%
!!! TGGTTTTTATGGTGTTATAT+GGG + chr4_3:72361060-72361079 Msa0661140:CDS 25.0%
! AGTAAAGGAGTCTTAATATT+GGG - chr4_3:72361142-72361161 None:intergenic 25.0%
TGAATGTAAAAGCAGGTATA+AGG - chr4_3:72359058-72359077 None:intergenic 30.0%
GATAAGAAAGAGAGATGTAT+AGG - chr4_3:72359103-72359122 None:intergenic 30.0%
TACATCTCTCTTTCTTATCT+TGG + chr4_3:72359104-72359123 Msa0661140:intron 30.0%
TATACTCCTTTAACAAATGG+AGG - chr4_3:72359336-72359355 None:intergenic 30.0%
! CTCTGATTTTTCTGCATTAT+TGG - chr4_3:72359541-72359560 None:intergenic 30.0%
! TACTGTAGAACCCAAAATTT+TGG - chr4_3:72359991-72360010 None:intergenic 30.0%
GGTTCTACAGTATCAATATT+AGG + chr4_3:72359999-72360018 Msa0661140:CDS 30.0%
GAAGAATCAATAATATGTGG+TGG - chr4_3:72360083-72360102 None:intergenic 30.0%
CTCAACATCACAATCAAATT+GGG + chr4_3:72360109-72360128 Msa0661140:CDS 30.0%
AGTATCTTATTGTGCCAATA+TGG + chr4_3:72360159-72360178 Msa0661140:CDS 30.0%
ACCTGAAAAATGTACCATAT+TGG - chr4_3:72360176-72360195 None:intergenic 30.0%
TATCAAACAATATCCATCAG+AGG + chr4_3:72360308-72360327 Msa0661140:CDS 30.0%
CTTAGTACTAGAACCAAATT+TGG + chr4_3:72360386-72360405 Msa0661140:intron 30.0%
ATGGAGATCATTGTTAAATC+AGG - chr4_3:72360429-72360448 None:intergenic 30.0%
AATGATCTCCATCATATACT+CGG + chr4_3:72360437-72360456 Msa0661140:intron 30.0%
CCCAACTTTCTCTTATAAAT+AGG - chr4_3:72360515-72360534 None:intergenic 30.0%
ATCATAAATAATAGGACTGG+AGG + chr4_3:72360556-72360575 Msa0661140:intron 30.0%
TCTTCTAGTGCTATATTTCT+TGG + chr4_3:72360849-72360868 Msa0661140:CDS 30.0%
ATCAAATGTTGCAGTGTAAT+CGG + chr4_3:72361019-72361038 Msa0661140:intron 30.0%
AATCGGAGCAATAATACTAT+CGG + chr4_3:72361036-72361055 Msa0661140:CDS 30.0%
!!! ATACTATCGGTTGGTTTTTA+TGG + chr4_3:72361049-72361068 Msa0661140:CDS 30.0%
!!! GGTTTTTATGGTGTTATATG+GGG + chr4_3:72361061-72361080 Msa0661140:CDS 30.0%
CTCTTCATTTGATTCTTCTT+TGG - chr4_3:72361092-72361111 None:intergenic 30.0%
CAGTAAAGGAGTCTTAATAT+TGG - chr4_3:72361143-72361162 None:intergenic 30.0%
CAAAACAATTGTTGAAGATG+TGG + chr4_3:72361199-72361218 Msa0661140:exon 30.0%
TGAATGTAAAAGCAGGTATA+AGG - chr4_3:72359058-72359077 None:intergenic 30.0%
GATAAGAAAGAGAGATGTAT+AGG - chr4_3:72359103-72359122 None:intergenic 30.0%
TACATCTCTCTTTCTTATCT+TGG + chr4_3:72359104-72359123 Msa0661140:intron 30.0%
TATACTCCTTTAACAAATGG+AGG - chr4_3:72359336-72359355 None:intergenic 30.0%
! CTCTGATTTTTCTGCATTAT+TGG - chr4_3:72359541-72359560 None:intergenic 30.0%
! TACTGTAGAACCCAAAATTT+TGG - chr4_3:72359991-72360010 None:intergenic 30.0%
GGTTCTACAGTATCAATATT+AGG + chr4_3:72359999-72360018 Msa0661140:CDS 30.0%
GAAGAATCAATAATATGTGG+TGG - chr4_3:72360083-72360102 None:intergenic 30.0%
CTCAACATCACAATCAAATT+GGG + chr4_3:72360109-72360128 Msa0661140:CDS 30.0%
AGTATCTTATTGTGCCAATA+TGG + chr4_3:72360159-72360178 Msa0661140:CDS 30.0%
ACCTGAAAAATGTACCATAT+TGG - chr4_3:72360176-72360195 None:intergenic 30.0%
TATCAAACAATATCCATCAG+AGG + chr4_3:72360308-72360327 Msa0661140:CDS 30.0%
CTTAGTACTAGAACCAAATT+TGG + chr4_3:72360386-72360405 Msa0661140:intron 30.0%
ATGGAGATCATTGTTAAATC+AGG - chr4_3:72360429-72360448 None:intergenic 30.0%
AATGATCTCCATCATATACT+CGG + chr4_3:72360437-72360456 Msa0661140:intron 30.0%
CCCAACTTTCTCTTATAAAT+AGG - chr4_3:72360515-72360534 None:intergenic 30.0%
ATCATAAATAATAGGACTGG+AGG + chr4_3:72360556-72360575 Msa0661140:intron 30.0%
TCTTCTAGTGCTATATTTCT+TGG + chr4_3:72360849-72360868 Msa0661140:CDS 30.0%
ATCAAATGTTGCAGTGTAAT+CGG + chr4_3:72361019-72361038 Msa0661140:intron 30.0%
AATCGGAGCAATAATACTAT+CGG + chr4_3:72361036-72361055 Msa0661140:CDS 30.0%
!!! ATACTATCGGTTGGTTTTTA+TGG + chr4_3:72361049-72361068 Msa0661140:CDS 30.0%
!!! GGTTTTTATGGTGTTATATG+GGG + chr4_3:72361061-72361080 Msa0661140:CDS 30.0%
CTCTTCATTTGATTCTTCTT+TGG - chr4_3:72361092-72361111 None:intergenic 30.0%
CAGTAAAGGAGTCTTAATAT+TGG - chr4_3:72361143-72361162 None:intergenic 30.0%
CAAAACAATTGTTGAAGATG+TGG + chr4_3:72361199-72361218 Msa0661140:exon 30.0%
TTTGATGAATTGTGTGAGTG+AGG + chr4_3:72358957-72358976 Msa0661140:intron 35.0%
TGTAAAAGCAGGTATAAGGT+TGG - chr4_3:72359054-72359073 None:intergenic 35.0%
GTATAGGTACCTGAAAAAGA+TGG - chr4_3:72359087-72359106 None:intergenic 35.0%
TATTAGACCTTCGTTGTGTT+CGG - chr4_3:72359419-72359438 None:intergenic 35.0%
! ATATATTGGCCGTTGATTGA+AGG + chr4_3:72359928-72359947 Msa0661140:intron 35.0%
! AAGCAGTCAAGCCAAAATTT+TGG + chr4_3:72359977-72359996 Msa0661140:CDS 35.0%
! AGCAGTCAAGCCAAAATTTT+GGG + chr4_3:72359978-72359997 Msa0661140:CDS 35.0%
! GATTTTGTGGGTATGATTGT+TGG - chr4_3:72360053-72360072 None:intergenic 35.0%
GCTCAACATCACAATCAAAT+TGG + chr4_3:72360108-72360127 Msa0661140:CDS 35.0%
TCACAATCAAATTGGGTTCT+TGG + chr4_3:72360116-72360135 Msa0661140:CDS 35.0%
! GCCAATATGGTACATTTTTC+AGG + chr4_3:72360172-72360191 Msa0661140:CDS 35.0%
ATATCCATCAGAGGTTATTG+TGG + chr4_3:72360317-72360336 Msa0661140:CDS 35.0%
AATACCACAATAACCTCTGA+TGG - chr4_3:72360324-72360343 None:intergenic 35.0%
! GGTTCTAGTACTAAGCATAT+TGG - chr4_3:72360381-72360400 None:intergenic 35.0%
CTACTTTACCGAGTATATGA+TGG - chr4_3:72360448-72360467 None:intergenic 35.0%
TCTCTTATAAATAGGATCGG+AGG - chr4_3:72360507-72360526 None:intergenic 35.0%
! TAAGTTGTCTTGTTCACACT+TGG + chr4_3:72360760-72360779 Msa0661140:CDS 35.0%
! AAGTTGTCTTGTTCACACTT+GGG + chr4_3:72360761-72360780 Msa0661140:CDS 35.0%
!!! CTTGGTGATGCACTATATTT+TGG + chr4_3:72360867-72360886 Msa0661140:CDS 35.0%
!!! GCACTATATTTTGGAACGTA+TGG + chr4_3:72360876-72360895 Msa0661140:intron 35.0%
AAAGGAGTCTTAATATTGGG+TGG - chr4_3:72361139-72361158 None:intergenic 35.0%
TATTAAGACTCCTTTACTGC+AGG + chr4_3:72361144-72361163 Msa0661140:CDS 35.0%
TTTGATGAATTGTGTGAGTG+AGG + chr4_3:72358957-72358976 Msa0661140:intron 35.0%
TGTAAAAGCAGGTATAAGGT+TGG - chr4_3:72359054-72359073 None:intergenic 35.0%
GTATAGGTACCTGAAAAAGA+TGG - chr4_3:72359087-72359106 None:intergenic 35.0%
TATTAGACCTTCGTTGTGTT+CGG - chr4_3:72359419-72359438 None:intergenic 35.0%
! ATATATTGGCCGTTGATTGA+AGG + chr4_3:72359928-72359947 Msa0661140:intron 35.0%
! AAGCAGTCAAGCCAAAATTT+TGG + chr4_3:72359977-72359996 Msa0661140:CDS 35.0%
! AGCAGTCAAGCCAAAATTTT+GGG + chr4_3:72359978-72359997 Msa0661140:CDS 35.0%
! GATTTTGTGGGTATGATTGT+TGG - chr4_3:72360053-72360072 None:intergenic 35.0%
GCTCAACATCACAATCAAAT+TGG + chr4_3:72360108-72360127 Msa0661140:CDS 35.0%
TCACAATCAAATTGGGTTCT+TGG + chr4_3:72360116-72360135 Msa0661140:CDS 35.0%
! GCCAATATGGTACATTTTTC+AGG + chr4_3:72360172-72360191 Msa0661140:CDS 35.0%
ATATCCATCAGAGGTTATTG+TGG + chr4_3:72360317-72360336 Msa0661140:CDS 35.0%
AATACCACAATAACCTCTGA+TGG - chr4_3:72360324-72360343 None:intergenic 35.0%
! GGTTCTAGTACTAAGCATAT+TGG - chr4_3:72360381-72360400 None:intergenic 35.0%
CTACTTTACCGAGTATATGA+TGG - chr4_3:72360448-72360467 None:intergenic 35.0%
TCTCTTATAAATAGGATCGG+AGG - chr4_3:72360507-72360526 None:intergenic 35.0%
! TAAGTTGTCTTGTTCACACT+TGG + chr4_3:72360760-72360779 Msa0661140:CDS 35.0%
! AAGTTGTCTTGTTCACACTT+GGG + chr4_3:72360761-72360780 Msa0661140:CDS 35.0%
!!! CTTGGTGATGCACTATATTT+TGG + chr4_3:72360867-72360886 Msa0661140:CDS 35.0%
!!! GCACTATATTTTGGAACGTA+TGG + chr4_3:72360876-72360895 Msa0661140:intron 35.0%
AAAGGAGTCTTAATATTGGG+TGG - chr4_3:72361139-72361158 None:intergenic 35.0%
TATTAAGACTCCTTTACTGC+AGG + chr4_3:72361144-72361163 Msa0661140:CDS 35.0%
GAAAGAGATGAAGCAAGAGT+AGG - chr4_3:72359032-72359051 None:intergenic 40.0%
GCAAGGATGAATGTAAAAGC+AGG - chr4_3:72359065-72359084 None:intergenic 40.0%
! TCATCCTTGCCATCTTTTTC+AGG + chr4_3:72359075-72359094 Msa0661140:CDS 40.0%
! ACAGCTCCTCCATTTGTTAA+AGG + chr4_3:72359327-72359346 Msa0661140:intron 40.0%
! ATTGATTCCGAACACAACGA+AGG + chr4_3:72359409-72359428 Msa0661140:intron 40.0%
TAAAGGCCTGGCTAACAAAT+TGG + chr4_3:72359514-72359533 Msa0661140:intron 40.0%
!! ATTGGTCCAATTTGTTAGCC+AGG - chr4_3:72359523-72359542 None:intergenic 40.0%
CTTCTCCATCCTTCAATCAA+CGG - chr4_3:72359940-72359959 None:intergenic 40.0%
!!! GGTGGTTGTGTTGATTTTGT+GGG - chr4_3:72360065-72360084 None:intergenic 40.0%
!!! TGGTGGTTGTGTTGATTTTG+TGG - chr4_3:72360066-72360085 None:intergenic 40.0%
CAATCAAATTGGGTTCTTGG+AGG + chr4_3:72360119-72360138 Msa0661140:CDS 40.0%
TAGAACCAAATTTGGGTGCT+TGG + chr4_3:72360394-72360413 Msa0661140:intron 40.0%
ATCTTCCAAGCACCCAAATT+TGG - chr4_3:72360402-72360421 None:intergenic 40.0%
AACAAGACAACTTAGTCCTG+TGG - chr4_3:72360754-72360773 None:intergenic 40.0%
! AGTTGTCTTGTTCACACTTG+GGG + chr4_3:72360762-72360781 Msa0661140:CDS 40.0%
ACTTGGGGACTCAATGTTAA+AGG + chr4_3:72360777-72360796 Msa0661140:CDS 40.0%
! GCAGCAATTGCTATTGACAA+AGG - chr4_3:72360828-72360847 None:intergenic 40.0%
GGAGCAATAATACTATCGGT+TGG + chr4_3:72361040-72361059 Msa0661140:CDS 40.0%
GAAAGAGATGAAGCAAGAGT+AGG - chr4_3:72359032-72359051 None:intergenic 40.0%
GCAAGGATGAATGTAAAAGC+AGG - chr4_3:72359065-72359084 None:intergenic 40.0%
! TCATCCTTGCCATCTTTTTC+AGG + chr4_3:72359075-72359094 Msa0661140:CDS 40.0%
! ACAGCTCCTCCATTTGTTAA+AGG + chr4_3:72359327-72359346 Msa0661140:intron 40.0%
! ATTGATTCCGAACACAACGA+AGG + chr4_3:72359409-72359428 Msa0661140:intron 40.0%
TAAAGGCCTGGCTAACAAAT+TGG + chr4_3:72359514-72359533 Msa0661140:intron 40.0%
!! ATTGGTCCAATTTGTTAGCC+AGG - chr4_3:72359523-72359542 None:intergenic 40.0%
CTTCTCCATCCTTCAATCAA+CGG - chr4_3:72359940-72359959 None:intergenic 40.0%
!!! GGTGGTTGTGTTGATTTTGT+GGG - chr4_3:72360065-72360084 None:intergenic 40.0%
!!! TGGTGGTTGTGTTGATTTTG+TGG - chr4_3:72360066-72360085 None:intergenic 40.0%
CAATCAAATTGGGTTCTTGG+AGG + chr4_3:72360119-72360138 Msa0661140:CDS 40.0%
TAGAACCAAATTTGGGTGCT+TGG + chr4_3:72360394-72360413 Msa0661140:intron 40.0%
ATCTTCCAAGCACCCAAATT+TGG - chr4_3:72360402-72360421 None:intergenic 40.0%
AACAAGACAACTTAGTCCTG+TGG - chr4_3:72360754-72360773 None:intergenic 40.0%
! AGTTGTCTTGTTCACACTTG+GGG + chr4_3:72360762-72360781 Msa0661140:CDS 40.0%
ACTTGGGGACTCAATGTTAA+AGG + chr4_3:72360777-72360796 Msa0661140:CDS 40.0%
! GCAGCAATTGCTATTGACAA+AGG - chr4_3:72360828-72360847 None:intergenic 40.0%
GGAGCAATAATACTATCGGT+TGG + chr4_3:72361040-72361059 Msa0661140:CDS 40.0%
AGAGTTGTAGCTCAGCTTTG+TGG + chr4_3:72358981-72359000 Msa0661140:intron 45.0%
! GCTCAGCTTTGTGGATATAG+AGG + chr4_3:72358990-72359009 Msa0661140:CDS 45.0%
GGTACCTGAAAAAGATGGCA+AGG - chr4_3:72359082-72359101 None:intergenic 45.0%
! ATTGGCCGTTGATTGAAGGA+TGG + chr4_3:72359932-72359951 Msa0661140:intron 45.0%
TGTTGTGTAGAGTGTGCAGT+GGG - chr4_3:72360207-72360226 None:intergenic 45.0%
TTGTTGTGTAGAGTGTGCAG+TGG - chr4_3:72360208-72360227 None:intergenic 45.0%
TAGACACTAGTGCTCCACAT+AGG - chr4_3:72360356-72360375 None:intergenic 45.0%
! ATGCAGGGATTCTTTTCCAC+AGG + chr4_3:72360735-72360754 Msa0661140:intron 45.0%
GGAGTCTTAATATTGGGTGG+AGG - chr4_3:72361136-72361155 None:intergenic 45.0%
ATGTTGGAGTCCTGCAGTAA+AGG - chr4_3:72361157-72361176 None:intergenic 45.0%
AGAGTTGTAGCTCAGCTTTG+TGG + chr4_3:72358981-72359000 Msa0661140:intron 45.0%
! GCTCAGCTTTGTGGATATAG+AGG + chr4_3:72358990-72359009 Msa0661140:CDS 45.0%
GGTACCTGAAAAAGATGGCA+AGG - chr4_3:72359082-72359101 None:intergenic 45.0%
! ATTGGCCGTTGATTGAAGGA+TGG + chr4_3:72359932-72359951 Msa0661140:intron 45.0%
TGTTGTGTAGAGTGTGCAGT+GGG - chr4_3:72360207-72360226 None:intergenic 45.0%
TTGTTGTGTAGAGTGTGCAG+TGG - chr4_3:72360208-72360227 None:intergenic 45.0%
TAGACACTAGTGCTCCACAT+AGG - chr4_3:72360356-72360375 None:intergenic 45.0%
! ATGCAGGGATTCTTTTCCAC+AGG + chr4_3:72360735-72360754 Msa0661140:intron 45.0%
GGAGTCTTAATATTGGGTGG+AGG - chr4_3:72361136-72361155 None:intergenic 45.0%
ATGTTGGAGTCCTGCAGTAA+AGG - chr4_3:72361157-72361176 None:intergenic 45.0%
!! ATAACTATATAATAATAAAA+AGG - chr4_3:72359471-72359490 None:intergenic 5.0%
!!! TTAATTTTTTTTTACATTTT+CGG + chr4_3:72360648-72360667 Msa0661140:intron 5.0%
!!! AAATTTTAAAAAAAAATTAG+GGG - chr4_3:72360910-72360929 None:intergenic 5.0%
!!! CAAAATTTTAAAAAAAAATT+AGG - chr4_3:72360912-72360931 None:intergenic 5.0%
!! ATAACTATATAATAATAAAA+AGG - chr4_3:72359471-72359490 None:intergenic 5.0%
!!! TTAATTTTTTTTTACATTTT+CGG + chr4_3:72360648-72360667 Msa0661140:intron 5.0%
!!! AAATTTTAAAAAAAAATTAG+GGG - chr4_3:72360910-72360929 None:intergenic 5.0%
!!! CAAAATTTTAAAAAAAAATT+AGG - chr4_3:72360912-72360931 None:intergenic 5.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 72358857 72361276 72358857 ID=Msa0661140;Name=Msa0661140
chr4_3 mRNA 72358857 72361276 72358857 ID=Msa0661140-mRNA-1;Parent=Msa0661140;Name=Msa0661140-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0|0.4|0.33|0.83|0.8|0.66|6|63|261
chr4_3 exon 72358857 72358861 72358857 ID=Msa0661140-mRNA-1:exon:21672;Parent=Msa0661140-mRNA-1
chr4_3 exon 72358983 72359096 72358983 ID=Msa0661140-mRNA-1:exon:21673;Parent=Msa0661140-mRNA-1
chr4_3 exon 72359950 72360193 72359950 ID=Msa0661140-mRNA-1:exon:21674;Parent=Msa0661140-mRNA-1
chr4_3 exon 72360300 72360389 72360300 ID=Msa0661140-mRNA-1:exon:21675;Parent=Msa0661140-mRNA-1
chr4_3 exon 72360741 72360892 72360741 ID=Msa0661140-mRNA-1:exon:21676;Parent=Msa0661140-mRNA-1
chr4_3 exon 72361033 72361276 72361033 ID=Msa0661140-mRNA-1:exon:21677;Parent=Msa0661140-mRNA-1
chr4_3 CDS 72358857 72358861 72358857 ID=Msa0661140-mRNA-1:cds;Parent=Msa0661140-mRNA-1
chr4_3 CDS 72358983 72359096 72358983 ID=Msa0661140-mRNA-1:cds;Parent=Msa0661140-mRNA-1
chr4_3 CDS 72359950 72360193 72359950 ID=Msa0661140-mRNA-1:cds;Parent=Msa0661140-mRNA-1
chr4_3 CDS 72360300 72360389 72360300 ID=Msa0661140-mRNA-1:cds;Parent=Msa0661140-mRNA-1
chr4_3 CDS 72360741 72360892 72360741 ID=Msa0661140-mRNA-1:cds;Parent=Msa0661140-mRNA-1
chr4_3 CDS 72361033 72361213 72361033 ID=Msa0661140-mRNA-1:cds;Parent=Msa0661140-mRNA-1
chr4_3 three_prime_UTR 72361214 72361276 72361214 ID=Msa0661140-mRNA-1:three_prime_utr;Parent=Msa0661140-mRNA-1
Gene Sequence

>Msa0661140

ATGGGAGTTGTAGCTCAGCTTTGTGGATATAGAGGACTTGAATACACAACACCTACTCTTGCTTCATCTCTTTCCAACCTTATACCTGCTTTTACATTCATCCTTGCCATCTTTTTCAGGATGGAGAAGATAGATTTAAGAAACAAAAGCAGTCAAGCCAAAATTTTGGGTTCTACAGTATCAATATTAGGTGCACTTATAATTGTTCTTTATAAGGGTCCAACAATCATACCCACAAAATCAACACAACCACCACATATTATTGATTCTTCAATAGGCTCAACATCACAATCAAATTGGGTTCTTGGAGGATCTCTACTTGTTATTGAGTATCTTATTGTGCCAATATGGTACATTTTTCAGACTAGTGTTATCAAACAATATCCATCAGAGGTTATTGTGGTATTTTTATACAACCTATGTGGAGCACTAGTGTCTATACCAATATGCTTAGGATTCTTTTCCACAGGACTAAGTTGTCTTGTTCACACTTGGGGACTCAATGTTAAAGGCCCTGTGTATATATCACTCTTTAAGCCTTTGTCAATAGCAATTGCTGCATCTTCTAGTGCTATATTTCTTGGTGATGCACTATATTTTGGAACTGTAATCGGAGCAATAATACTATCGGTTGGTTTTTATGGTGTTATATGGGGAAAAGCCAAAGAAGAATCAAATGAAGAGTTTGACATAGTTAGTGTTTCACCTCCACCCAATATTAAGACTCCTTTACTGCAGGACTCCAACATAATAGATAGAGAAAAAACAACACAAAACAATTGTTGA
Protein sequence

>Msa0661140

MGVVAQLCGYRGLEYTTPTLASSLSNLIPAFTFILAIFFRMEKIDLRNKSSQAKILGSTVSILGALIIVLYKGPTIIPTKSTQPPHIIDSSIGSTSQSNWVLGGSLLVIEYLIVPIWYIFQTSVIKQYPSEVIVVFLYNLCGALVSIPICLGFFSTGLSCLVHTWGLNVKGPVYISLFKPLSIAIAASSSAIFLGDALYFGTVIGAIILSVGFYGVIWGKAKEESNEEFDIVSVSPPPNIKTPLLQDSNIIDREKTTQNNC*