AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0336460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0336460 MtrunA17_Chr2g0322861 93.865 163 9 1 18 179 225 387 7.97e-109 316
Msa0336460 MtrunA17_Chr4g0024121 70.370 162 46 2 18 178 166 326 6.12e-80 240
Msa0336460 MtrunA17_Chr7g0268501 54.321 162 70 3 18 178 162 320 3.84e-54 174
Msa0336460 MtrunA17_Chr7g0268521 54.321 162 70 3 18 178 162 320 3.84e-54 174
Msa0336460 MtrunA17_Chr2g0292261 48.101 158 78 3 23 178 175 330 4.00e-40 139
Msa0336460 MtrunA17_Chr5g0402581 46.053 152 81 1 27 178 176 326 9.73e-40 137
Msa0336460 MtrunA17_Chr3g0128061 48.734 158 78 3 23 179 172 327 7.53e-39 135
Msa0336460 MtrunA17_Chr5g0402571 43.421 152 85 1 27 178 176 326 2.81e-37 130
Msa0336460 MtrunA17_Chr2g0292241 44.654 159 84 3 23 179 175 331 1.67e-36 129
Msa0336460 MtrunA17_Chr5g0401431 42.581 155 87 2 23 177 176 328 1.62e-35 126
Msa0336460 MtrunA17_Chr2g0292211 43.038 158 86 3 23 178 186 341 1.28e-34 124
Msa0336460 MtrunA17_Chr2g0292181 42.405 158 87 3 23 178 184 339 3.45e-34 123
Msa0336460 MtrunA17_Chr2g0279061 43.226 155 84 2 27 178 113 266 6.14e-34 120
Msa0336460 MtrunA17_Chr2g0292251 42.405 158 87 3 23 178 174 329 6.79e-34 122
Msa0336460 MtrunA17_Chr1g0164891 43.506 154 82 4 27 178 242 392 9.30e-34 123
Msa0336460 MtrunA17_Chr2g0279041 43.226 155 84 2 27 178 172 325 1.68e-33 121
Msa0336460 MtrunA17_Chr2g0292291 44.025 159 84 4 23 178 176 332 2.41e-33 121
Msa0336460 MtrunA17_Chr5g0404721 44.654 159 85 3 22 179 175 331 4.33e-33 120
Msa0336460 MtrunA17_Chr2g0291751 41.139 158 89 3 23 178 175 330 9.19e-33 119
Msa0336460 MtrunA17_Chr2g0292271 43.671 158 85 3 23 178 175 330 1.95e-32 119
Msa0336460 MtrunA17_Chr1g0148021 40.113 177 101 3 5 178 154 328 2.36e-32 118
Msa0336460 MtrunA17_Chr4g0070951 40.994 161 89 3 22 178 171 329 2.37e-32 118
Msa0336460 MtrunA17_Chr4g0072091 41.975 162 92 2 17 178 164 323 2.92e-32 117
Msa0336460 MtrunA17_Chr8g0372601 42.675 157 89 1 22 178 175 330 5.89e-32 117
Msa0336460 MtrunA17_Chr4g0013181 40.994 161 83 4 21 178 202 353 2.76e-31 115
Msa0336460 MtrunA17_Chr2g0299691 42.593 162 86 3 22 178 199 358 3.11e-31 115
Msa0336460 MtrunA17_Chr8g0371381 42.949 156 83 4 23 178 170 319 4.16e-31 114
Msa0336460 MtrunA17_Chr7g0225221 38.462 156 90 3 23 178 171 320 9.84e-31 113
Msa0336460 MtrunA17_Chr4g0013171 40.373 161 84 4 21 178 170 321 1.56e-30 113
Msa0336460 MtrunA17_Chr2g0292101 42.405 158 87 3 23 178 171 326 2.96e-30 112
Msa0336460 MtrunA17_Chr2g0292111 40.476 168 93 4 16 178 165 330 3.36e-30 113
Msa0336460 MtrunA17_Chr5g0405991 44.654 159 76 6 23 178 167 316 4.53e-30 112
Msa0336460 MtrunA17_Chr1g0175561 38.961 154 90 3 27 178 175 326 2.03e-29 110
Msa0336460 MtrunA17_Chr2g0292151 39.873 158 91 3 23 178 175 330 2.16e-29 110
Msa0336460 MtrunA17_Chr7g0258811 40.260 154 88 3 27 178 220 371 3.23e-29 110
Msa0336460 MtrunA17_Chr1g0194151 40.645 155 86 4 27 178 182 333 4.23e-29 109
Msa0336460 MtrunA17_Chr2g0292281 42.208 154 75 5 27 178 75 216 5.10e-29 107
Msa0336460 MtrunA17_Chr4g0053871 39.355 155 87 3 23 177 176 323 7.69e-29 108
Msa0336460 MtrunA17_Chr1g0194271 38.509 161 88 3 27 178 182 340 8.67e-29 108
Msa0336460 MtrunA17_Chr4g0046131 39.103 156 91 4 23 176 191 344 1.41e-28 108
Msa0336460 MtrunA17_Chr1g0204011 38.816 152 89 3 27 178 175 322 1.63e-28 107
Msa0336460 MtrunA17_Chr2g0292091 39.873 158 91 3 23 178 170 325 1.16e-27 105
Msa0336460 MtrunA17_Chr4g0076211 40.741 162 89 5 19 178 177 333 1.63e-27 105
Msa0336460 MtrunA17_Chr2g0296621 38.372 172 98 5 9 178 176 341 1.41e-26 103
Msa0336460 MtrunA17_Chr1g0197411 35.570 149 93 3 27 174 180 326 1.76e-26 102
Msa0336460 MtrunA17_Chr6g0467161 38.994 159 87 5 20 178 188 336 2.32e-26 102
Msa0336460 MtrunA17_Chr1g0155611 41.772 158 88 3 23 178 192 347 6.34e-26 101
Msa0336460 MtrunA17_Chr1g0157051 41.401 157 83 7 23 177 168 317 9.28e-26 100
Msa0336460 MtrunA17_Chr2g0320071 38.217 157 88 3 22 178 170 317 1.93e-25 99.8
Msa0336460 MtrunA17_Chr4g0063671 40.385 156 88 3 27 178 166 320 2.02e-25 99.8
Msa0336460 MtrunA17_Chr2g0329641 35.669 157 94 3 27 178 181 335 3.30e-25 99.4
Msa0336460 MtrunA17_Chr6g0467121 41.935 155 77 5 26 178 220 363 4.59e-25 99.8
Msa0336460 MtrunA17_Chr2g0320101 37.580 157 89 3 22 178 170 317 5.41e-25 98.6
Msa0336460 MtrunA17_Chr3g0114451 36.943 157 90 3 22 178 171 318 5.44e-25 98.6
Msa0336460 MtrunA17_Chr2g0320061 37.975 158 88 4 22 178 170 318 1.03e-24 97.8
Msa0336460 MtrunA17_Chr8g0364291 34.194 155 99 3 26 179 173 325 1.07e-24 97.8
Msa0336460 MtrunA17_Chr7g0244461 35.897 156 91 3 23 178 166 312 1.61e-24 97.1
Msa0336460 MtrunA17_Chr3g0106921 37.107 159 89 4 22 178 171 320 1.68e-24 97.4
Msa0336460 MtrunA17_Chr3g0129051 37.662 154 85 4 23 174 174 318 1.71e-24 97.4
Msa0336460 MtrunA17_Chr4g0043391 35.443 158 96 4 22 178 172 324 2.64e-24 96.7
Msa0336460 MtrunA17_Chr7g0244451 36.184 152 88 3 27 178 174 316 3.05e-24 96.7
Msa0336460 MtrunA17_Chr2g0320051 38.650 163 80 5 22 178 170 318 3.98e-24 96.3
Msa0336460 MtrunA17_Chr6g0460711 36.774 155 89 4 26 178 167 314 4.92e-24 95.9
Msa0336460 MtrunA17_Chr4g0038171 35.443 158 96 4 22 178 169 321 1.04e-23 95.1
Msa0336460 MtrunA17_Chr5g0432341 39.024 164 87 6 18 178 168 321 1.42e-23 94.7
Msa0336460 MtrunA17_Chr2g0278671 38.125 160 93 3 23 178 166 323 1.45e-23 94.7
Msa0336460 MtrunA17_Chr5g0432351 39.634 164 86 6 18 178 168 321 1.46e-23 94.7
Msa0336460 MtrunA17_Chr3g0129061 36.538 156 86 5 22 174 173 318 2.85e-23 94.0
Msa0336460 MtrunA17_Chr6g0467251 36.306 157 91 4 23 179 182 329 5.40e-23 93.6
Msa0336460 MtrunA17_Chr3g0114461 36.076 158 91 4 22 178 44 192 5.93e-23 90.5
Msa0336460 MtrunA17_Chr5g0432441 38.710 155 83 4 27 178 181 326 6.44e-23 93.2
Msa0336460 MtrunA17_Chr3g0106901 34.615 156 93 3 23 178 177 323 7.45e-23 92.8
Msa0336460 MtrunA17_Chr2g0320091 36.943 157 89 4 23 178 171 318 8.49e-23 92.8
Msa0336460 MtrunA17_Chr3g0107881 38.182 165 88 5 19 178 169 324 1.12e-22 92.4
Msa0336460 MtrunA17_Chr4g0038161 33.125 160 96 4 22 178 169 320 1.41e-22 92.0
Msa0336460 MtrunA17_Chr4g0013191 34.395 157 94 3 23 178 192 340 2.36e-22 91.7
Msa0336460 MtrunA17_Chr4g0038151 33.758 157 99 3 22 178 170 321 2.89e-22 91.3
Msa0336460 MtrunA17_Chr1g0188431 32.298 161 98 4 18 178 178 327 2.92e-22 91.3
Msa0336460 MtrunA17_Chr7g0254201 33.962 159 95 4 22 178 162 312 1.06e-21 89.7
Msa0336460 MtrunA17_Chr5g0432331 37.195 164 90 6 18 178 199 352 1.76e-21 89.7
Msa0336460 MtrunA17_Chr5g0400331 33.529 170 98 4 20 178 166 331 2.28e-21 89.0
Msa0336460 MtrunA17_Chr4g0013721 37.342 158 94 4 22 179 245 397 2.71e-21 89.7
Msa0336460 MtrunA17_Chr3g0129041 36.478 159 90 4 22 178 173 322 2.94e-21 88.6
Msa0336460 MtrunA17_Chr4g0068621 36.364 154 91 5 27 178 188 336 6.54e-21 87.8
Msa0336460 MtrunA17_Chr4g0013821 35.669 157 96 4 22 178 173 324 7.32e-21 87.8
Msa0336460 MtrunA17_Chr2g0320031 33.544 158 95 4 22 178 171 319 2.06e-20 86.3
Msa0336460 MtrunA17_Chr4g0013201 34.615 156 93 3 23 178 192 338 4.84e-20 85.5
Msa0336460 MtrunA17_Chr5g0438021 35.000 160 89 5 23 178 177 325 6.24e-20 85.1
Msa0336460 MtrunA17_Chr6g0451571 32.692 156 98 5 25 178 184 334 1.62e-19 84.0
Msa0336460 MtrunA17_Chr6g0477071 36.184 152 86 6 27 178 202 342 1.66e-19 84.0
Msa0336460 MtrunA17_Chr7g0268491 43.678 87 47 1 92 178 13 97 1.95e-19 78.6
Msa0336460 MtrunA17_Chr1g0213061 34.591 159 92 5 23 178 172 321 3.16e-19 83.2
Msa0336460 MtrunA17_Chr7g0224371 36.842 152 86 4 27 178 181 322 2.11e-18 80.9
Msa0336460 MtrunA17_Chr3g0136751 37.419 155 93 3 26 178 170 322 1.17e-17 79.0
Msa0336460 MtrunA17_Chr7g0254231 32.278 158 97 5 22 179 165 312 1.42e-17 78.6
Msa0336460 MtrunA17_Chr2g0311251 28.177 181 100 2 26 177 160 339 8.40e-17 76.6
Msa0336460 MtrunA17_Chr5g0421411 33.540 161 92 8 27 178 131 285 8.94e-17 75.9
Msa0336460 MtrunA17_Chr4g0074851 30.968 155 97 5 27 178 181 328 1.43e-16 75.9
Msa0336460 MtrunA17_Chr5g0432321 40.625 96 48 3 27 122 46 132 1.87e-16 72.0
Msa0336460 MtrunA17_Chr2g0311241 28.729 181 99 3 26 177 250 429 2.31e-16 75.9
Msa0336460 MtrunA17_Chr4g0004591 29.240 171 98 4 27 177 188 355 3.11e-16 75.1
Msa0336460 MtrunA17_Chr5g0413051 28.402 169 113 4 16 178 211 377 7.22e-16 74.3
Msa0336460 MtrunA17_Chr2g0316481 28.492 179 98 4 26 175 226 403 1.51e-14 70.5
Msa0336460 MtrunA17_Chr2g0316791 27.374 179 100 3 26 175 226 403 3.82e-14 69.3
Msa0336460 MtrunA17_Chr2g0316831 27.869 183 101 4 22 175 259 439 5.27e-14 68.9
Msa0336460 MtrunA17_Chr4g0075241 28.205 156 105 4 25 178 177 327 2.51e-11 60.8
Msa0336460 MtrunA17_Chr1g0181501 29.299 157 100 5 25 178 41 189 2.68e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0336460 AT1G05260.1 71.338 157 45 0 23 179 170 326 6.47e-81 243
Msa0336460 AT4G11290.1 69.753 162 48 1 18 178 165 326 1.31e-75 229
Msa0336460 AT3G21770.1 66.667 156 52 0 22 177 172 327 8.32e-71 217
Msa0336460 AT3G01190.1 56.790 162 67 3 18 178 162 321 1.03e-51 168
Msa0336460 AT5G15180.1 55.414 157 68 2 22 178 175 329 5.35e-50 164
Msa0336460 AT1G05240.1 49.686 159 76 3 22 178 169 325 2.48e-41 142
Msa0336460 AT1G05250.1 49.686 159 76 3 22 178 169 325 2.48e-41 142
Msa0336460 AT2G39040.1 48.765 162 75 5 22 178 192 350 1.34e-38 135
Msa0336460 AT5G14130.1 44.231 156 84 3 23 178 178 330 5.60e-37 130
Msa0336460 AT5G14130.2 44.231 156 84 3 23 178 219 371 1.49e-36 130
Msa0336460 AT2G18150.1 43.396 159 87 3 22 179 181 337 5.85e-36 128
Msa0336460 AT2G18980.1 42.949 156 87 2 23 178 170 323 9.39e-35 124
Msa0336460 AT5G64120.1 41.290 155 88 3 24 178 177 328 1.22e-33 122
Msa0336460 AT2G18140.1 42.208 154 86 3 27 179 185 336 2.18e-33 121
Msa0336460 AT4G36430.1 40.854 164 91 4 20 179 170 331 6.40e-32 117
Msa0336460 AT4G37530.1 41.026 156 90 2 23 178 176 329 1.37e-31 116
Msa0336460 AT5G19880.1 41.509 159 88 4 23 178 173 329 2.84e-31 115
Msa0336460 AT2G24800.1 39.103 156 90 3 26 178 176 329 1.03e-30 114
Msa0336460 AT5G66390.1 42.767 159 88 3 22 179 178 334 1.92e-30 113
Msa0336460 AT4G37520.2 39.744 156 92 2 23 178 173 326 6.45e-30 112
Msa0336460 AT4G37520.1 39.744 156 92 2 23 178 176 329 7.23e-30 112
Msa0336460 AT3G50990.1 40.506 158 93 1 22 179 186 342 1.27e-29 111
Msa0336460 AT5G39580.1 38.514 148 87 4 32 178 175 319 5.02e-29 109
Msa0336460 AT1G49570.1 41.176 153 85 4 27 176 198 348 1.07e-28 109
Msa0336460 AT5G06730.1 40.260 154 88 3 27 178 183 334 1.45e-28 108
Msa0336460 AT5G64110.1 37.013 154 94 2 27 178 178 330 2.93e-28 107
Msa0336460 AT4G30170.1 38.462 156 94 2 23 178 172 325 3.03e-28 107
Msa0336460 AT2G38390.1 39.623 159 91 4 23 178 177 333 5.72e-28 107
Msa0336460 AT1G71695.1 41.447 152 81 4 27 178 196 339 6.44e-28 107
Msa0336460 AT4G31760.1 38.182 165 93 5 20 178 165 326 1.86e-27 105
Msa0336460 AT5G51890.1 44.516 155 77 6 27 178 174 322 3.66e-27 104
Msa0336460 AT5G06720.1 37.791 172 101 4 9 178 166 333 6.12e-27 104
Msa0336460 AT3G49120.1 38.554 166 93 5 21 179 173 336 1.01e-26 103
Msa0336460 AT2G38380.1 38.788 165 95 5 18 178 171 333 1.01e-26 103
Msa0336460 AT3G49110.1 38.554 166 93 5 21 179 174 337 1.02e-26 103
Msa0336460 AT5G67400.1 36.538 156 97 2 23 178 176 329 3.15e-26 102
Msa0336460 AT3G32980.1 37.349 166 95 5 21 179 172 335 3.97e-26 102
Msa0336460 AT2G41480.1 39.103 156 90 3 27 178 174 328 5.00e-26 101
Msa0336460 AT1G30870.1 38.065 155 90 3 27 179 196 346 5.14e-26 102
Msa0336460 AT5G64100.1 39.355 155 87 4 27 178 181 331 8.87e-26 101
Msa0336460 AT1G44970.1 39.241 158 92 3 23 178 191 346 2.69e-25 100
Msa0336460 AT5G17820.1 35.714 154 93 2 25 178 166 313 5.89e-25 98.6
Msa0336460 AT5G17820.2 35.714 154 93 2 25 178 219 366 9.04e-25 99.0
Msa0336460 AT5G24070.1 34.395 157 96 3 27 178 181 335 1.16e-24 98.2
Msa0336460 AT5G58390.1 38.065 155 83 5 27 178 172 316 1.42e-24 97.8
Msa0336460 AT4G08770.1 38.554 166 93 5 21 179 165 328 1.50e-24 98.2
Msa0336460 AT1G14540.1 35.032 157 93 4 23 178 167 315 1.55e-24 97.8
Msa0336460 AT4G17690.1 36.424 151 88 4 25 174 175 318 2.10e-24 97.4
Msa0336460 AT1G14550.1 35.669 157 92 4 23 178 173 321 3.56e-24 96.7
Msa0336460 AT3G49960.1 35.256 156 99 2 23 178 176 329 4.55e-24 96.7
Msa0336460 AT3G03670.1 35.065 154 98 2 25 178 170 321 5.56e-24 96.3
Msa0336460 AT4G25980.1 35.404 161 97 3 23 178 168 326 1.28e-23 95.5
Msa0336460 AT5G19890.1 35.669 157 93 4 27 179 176 328 2.29e-23 94.7
Msa0336460 AT4G08780.1 37.267 161 95 4 23 179 170 328 9.03e-23 93.2
Msa0336460 AT2G34060.1 34.641 153 98 2 23 174 191 342 9.70e-23 93.2
Msa0336460 AT2G43480.1 30.220 182 110 3 1 178 167 335 1.47e-22 92.8
Msa0336460 AT5G47000.1 37.037 162 87 6 23 179 180 331 1.95e-22 92.4
Msa0336460 AT1G77100.1 36.646 161 91 4 22 178 166 319 3.58e-22 91.3
Msa0336460 AT2G22420.1 37.179 156 93 4 23 178 169 319 3.85e-22 91.3
Msa0336460 AT5G42180.1 34.884 172 104 4 9 177 150 316 1.20e-21 89.7
Msa0336460 AT1G24110.1 34.395 157 97 4 25 179 171 323 4.12e-21 88.6
Msa0336460 AT4G37530.2 41.121 107 62 1 23 129 176 281 9.61e-21 87.0
Msa0336460 AT5G58400.1 35.220 159 90 5 23 178 177 325 1.08e-20 87.4
Msa0336460 AT5G22410.1 32.450 151 96 4 27 174 174 321 2.57e-20 86.7
Msa0336460 AT4G16270.1 33.146 178 111 5 5 178 175 348 1.60e-19 84.3
Msa0336460 AT4G33420.1 34.868 152 88 4 27 178 185 325 2.48e-19 83.6
Msa0336460 AT4G33420.2 35.256 156 89 5 23 178 229 372 3.84e-19 83.6
Msa0336460 AT4G26010.1 33.548 155 90 5 26 179 168 310 4.17e-19 82.8
Msa0336460 AT4G33870.1 31.522 184 89 4 27 175 217 398 4.41e-19 83.6
Msa0336460 AT4G26010.2 33.548 155 90 5 26 179 196 338 5.31e-19 82.8
Msa0336460 AT5G05340.1 32.484 157 97 3 23 178 176 324 6.83e-19 82.4
Msa0336460 AT5G40150.1 32.895 152 96 2 27 178 183 328 8.52e-19 82.4
Msa0336460 AT2G35380.2 30.000 160 100 3 27 178 92 247 9.62e-19 80.9
Msa0336460 AT2G35380.1 30.000 160 100 3 27 178 180 335 3.47e-18 80.9
Msa0336460 AT1G34510.1 31.169 154 93 5 27 179 169 310 3.64e-18 80.5
Msa0336460 AT3G28200.1 32.680 153 94 4 27 178 172 316 3.99e-18 80.5
Msa0336460 AT1G68850.1 28.662 157 106 4 27 178 180 335 4.35e-15 72.0
Msa0336460 AT3G17070.2 29.747 158 108 3 22 178 121 276 1.42e-14 70.1
Msa0336460 AT2G37130.2 30.323 155 98 4 27 178 149 296 2.38e-14 69.7
Msa0336460 AT3G17070.1 29.747 158 108 3 22 178 184 339 3.08e-14 69.7
Msa0336460 AT2G37130.1 30.323 155 98 4 27 178 180 327 3.13e-14 69.7

Find 66 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAAATCAATGGAGAAAA+TGG 0.079582 2_4:-55874027 Msa0336460:CDS
GAAATACAAGGATCCATTTA+AGG 0.201889 2_4:+55873592 None:intergenic
CCTCCAAATCTTACTGTTAG+AGG 0.255087 2_4:-55874948 Msa0336460:five_prime_UTR
ATCAAATGTGTTTCGACTTC+CGG 0.258605 2_4:+55874184 None:intergenic
CTTGATTGGCAAAGAGAGTT+TGG 0.269943 2_4:+55874571 None:intergenic
ACTTTCATGATTGCTTTGTT+AGG 0.271511 2_4:-55875125 Msa0336460:five_prime_UTR
AACCAAGGTGCATCCTTAAA+TGG 0.288049 2_4:-55873605 Msa0336460:three_prime_UTR
GAGTTTGGAGGGTGAAGTTT+TGG 0.298472 2_4:+55874586 None:intergenic
GCAATGATTAGTTCAGCTTT+TGG 0.305474 2_4:+55875210 None:intergenic
GAAGGTTGGTTGGTTACATT+AGG 0.327799 2_4:-55873700 Msa0336460:three_prime_UTR
TCAAATGTGTTTCGACTTCC+GGG 0.337076 2_4:+55874185 None:intergenic
TTCCAGTAAAATTGTATAAA+CGG 0.349083 2_4:+55874309 None:intergenic
CTTTCATGATTGCTTTGTTA+GGG 0.352765 2_4:-55875124 Msa0336460:intron
GGAAAGTTCCAACAGGTAGA+AGG 0.373285 2_4:-55874663 Msa0336460:five_prime_UTR
GATTTCAGGGTGGTCCATAT+TGG 0.381350 2_4:-55874684 Msa0336460:intron
ATATATTATTGACTCTGCTT+TGG 0.382069 2_4:+55873728 None:intergenic
CCTGTTGGAACTTTCCAATA+TGG 0.384950 2_4:+55874670 None:intergenic
AAGCCTCTAACAGTAAGATT+TGG 0.388544 2_4:+55874945 None:intergenic
GAGAGAAAATTTCTAGTGTT+AGG 0.395728 2_4:-55873758 Msa0336460:three_prime_UTR
GTCAATAATATATGTGTAGA+AGG 0.401632 2_4:-55873718 Msa0336460:three_prime_UTR
AAGTTTCAAGTGAGCCTTGA+AGG 0.403365 2_4:+55874063 None:intergenic
AGTCGAAACACATTTGATCT+TGG 0.404166 2_4:-55874179 Msa0336460:CDS
CAGGGTCACAAGGTGTGATT+AGG 0.404815 2_4:-55873986 Msa0336460:CDS
CAATACTACAATAGTCGAAA+TGG 0.408185 2_4:-55874210 Msa0336460:CDS
CTTGTGAATCAATTCCTTCA+AGG 0.416074 2_4:-55874077 Msa0336460:CDS
AAGGTTGGTTGGTTACATTA+GGG 0.425873 2_4:-55873699 Msa0336460:three_prime_UTR
AAATCAGTGTAATTACTTCA+AGG 0.442898 2_4:-55875304 Msa0336460:five_prime_UTR
CAAACTCTCTTTGCCAATCA+AGG 0.450028 2_4:-55874570 Msa0336460:CDS
TGCTGAATTTGCAAAATCAA+TGG 0.452311 2_4:-55874036 Msa0336460:CDS
TGTGGCTGCAATAGAGTCTC+TGG 0.459357 2_4:+55874839 None:intergenic
GCAAAATCAATGGAGAAAAT+GGG 0.466453 2_4:-55874026 Msa0336460:CDS
CCTCTAACAGTAAGATTTGG+AGG 0.486565 2_4:+55874948 None:intergenic
TGGAGTCGTATCGAATTTGT+TGG 0.490287 2_4:-55874638 Msa0336460:CDS
ATAATATATGTGTAGAAGGT+TGG 0.490676 2_4:-55873714 Msa0336460:three_prime_UTR
AAAGACTTAGTCCTCCTCTC+TGG 0.492430 2_4:-55874537 Msa0336460:intron
TGTTGGTTTGTTGAGTTCAA+AGG 0.507278 2_4:+55874987 None:intergenic
CCATATTGGAAAGTTCCAAC+AGG 0.513974 2_4:-55874670 Msa0336460:five_prime_UTR
GGACAAGTTAAAGTCAAGAC+AGG 0.523583 2_4:-55874005 Msa0336460:CDS
CTTGTTGAAGCTGAATGCCC+TGG 0.535811 2_4:-55874897 Msa0336460:five_prime_UTR
AGTCAAGTTGTCAAGAGAAG+AGG 0.538282 2_4:-55874149 Msa0336460:CDS
TATATGTGTAGAAGGTTGGT+TGG 0.540580 2_4:-55873710 Msa0336460:three_prime_UTR
GTTTGAAAATGATGTGCAAA+GGG 0.541770 2_4:+55874331 None:intergenic
ACTCCATCCCTTCTACCTGT+TGG 0.553125 2_4:+55874655 None:intergenic
ACATAATGTACCACCAAACC+AGG 0.555806 2_4:+55873808 None:intergenic
GAAAGTTCCAACAGGTAGAA+GGG 0.557568 2_4:-55874662 Msa0336460:five_prime_UTR
GTTCCAACAGGTAGAAGGGA+TGG 0.566917 2_4:-55874658 Msa0336460:exon
GATTGGCAAAGAGAGTTTGG+AGG 0.571052 2_4:+55874574 None:intergenic
ATTAATGCAGCAGCAAGTGA+TGG 0.574993 2_4:+55875156 None:intergenic
ATTGGCAAAGAGAGTTTGGA+GGG 0.582534 2_4:+55874575 None:intergenic
GCATATTCACTATCAAGTGA+TGG 0.604475 2_4:+55874272 None:intergenic
TCAGCACAAGAAACAACACC+AGG 0.606055 2_4:+55874879 None:intergenic
ACTCATGCTCAATTGCAGCT+AGG 0.609605 2_4:-55875252 Msa0336460:five_prime_UTR
ACAATAGTCGAAATGGACCC+CGG 0.613598 2_4:-55874203 Msa0336460:CDS
GGTTTGAAAATGATGTGCAA+AGG 0.614722 2_4:+55874330 None:intergenic
AACTAATGTATTGAAATACA+AGG 0.615863 2_4:+55873580 None:intergenic
ATCCATTTAAGGATGCACCT+TGG 0.617866 2_4:+55873603 None:intergenic
CAGGGCATTCAGCTTCAACA+AGG 0.628010 2_4:+55874898 None:intergenic
CAGCAAGTGATGGAGCATTG+TGG 0.631408 2_4:+55875166 None:intergenic
ATGAGAAGTTCTCTAAACCA+AGG 0.647882 2_4:-55873620 Msa0336460:three_prime_UTR
AAAGTCAAGACAGGGTCACA+AGG 0.649840 2_4:-55873996 Msa0336460:CDS
TATTGAGACACAACATGACA+TGG 0.653705 2_4:+55873887 None:intergenic
ATGTAGTATTACCAGAGAGG+AGG 0.667777 2_4:+55874526 None:intergenic
TGAATGTAGTATTACCAGAG+AGG 0.695454 2_4:+55874523 None:intergenic
CAAATGTGTTTCGACTTCCG+GGG 0.706421 2_4:+55874186 None:intergenic
GACAAGTTAAAGTCAAGACA+GGG 0.712891 2_4:-55874004 Msa0336460:CDS
CAGCACAAGAAACAACACCA+GGG 0.760815 2_4:+55874880 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATACTTTCATTTCATTTT+TGG - chr2_4:55873878-55873897 Msa0336460:three_prime_UTR 15.0%
!!! TAATAATTTTAAGAACAAGT+GGG - chr2_4:55875098-55875117 Msa0336460:intron 15.0%
!!! TTATACTTTCATTTCATTTT+TGG - chr2_4:55873878-55873897 Msa0336460:three_prime_UTR 15.0%
!!! TAATAATTTTAAGAACAAGT+GGG - chr2_4:55875098-55875117 Msa0336460:intron 15.0%
!!! AACATTAAGCATTTTGATTT+AGG - chr2_4:55873927-55873946 Msa0336460:three_prime_UTR 20.0%
!!! ACATTAAGCATTTTGATTTA+GGG - chr2_4:55873928-55873947 Msa0336460:three_prime_UTR 20.0%
!!! ACCATGATTTAAATTTTTTC+AGG - chr2_4:55874579-55874598 Msa0336460:CDS 20.0%
!! ACCTGAAAAAATTTAAATCA+TGG + chr2_4:55874583-55874602 None:intergenic 20.0%
!! TTCCAGTAAAATTGTATAAA+CGG + chr2_4:55874647-55874666 None:intergenic 20.0%
!!! TTTATACAATTTTACTGGAA+AGG - chr2_4:55874647-55874666 Msa0336460:exon 20.0%
!!! TTATACAATTTTACTGGAAA+GGG - chr2_4:55874648-55874667 Msa0336460:exon 20.0%
!!! TTTATGTTGATTTGTGTTTT+TGG - chr2_4:55875024-55875043 Msa0336460:intron 20.0%
!!! TTATGTTGATTTGTGTTTTT+GGG - chr2_4:55875025-55875044 Msa0336460:intron 20.0%
!!! GTAATAATTTTAAGAACAAG+TGG - chr2_4:55875097-55875116 Msa0336460:intron 20.0%
!!! CAATTGTAATTTTTATTTCC+TGG - chr2_4:55875127-55875146 Msa0336460:five_prime_UTR 20.0%
!! AACTAATGTATTGAAATACA+AGG + chr2_4:55875376-55875395 None:intergenic 20.0%
!!! AACATTAAGCATTTTGATTT+AGG - chr2_4:55873927-55873946 Msa0336460:three_prime_UTR 20.0%
!!! ACATTAAGCATTTTGATTTA+GGG - chr2_4:55873928-55873947 Msa0336460:three_prime_UTR 20.0%
!!! ACCATGATTTAAATTTTTTC+AGG - chr2_4:55874579-55874598 Msa0336460:CDS 20.0%
!! ACCTGAAAAAATTTAAATCA+TGG + chr2_4:55874583-55874602 None:intergenic 20.0%
!! TTCCAGTAAAATTGTATAAA+CGG + chr2_4:55874647-55874666 None:intergenic 20.0%
!!! TTTATACAATTTTACTGGAA+AGG - chr2_4:55874647-55874666 Msa0336460:exon 20.0%
!!! TTATACAATTTTACTGGAAA+GGG - chr2_4:55874648-55874667 Msa0336460:exon 20.0%
!!! TTTATGTTGATTTGTGTTTT+TGG - chr2_4:55875024-55875043 Msa0336460:intron 20.0%
!!! TTATGTTGATTTGTGTTTTT+GGG - chr2_4:55875025-55875044 Msa0336460:intron 20.0%
!!! GTAATAATTTTAAGAACAAG+TGG - chr2_4:55875097-55875116 Msa0336460:intron 20.0%
!!! CAATTGTAATTTTTATTTCC+TGG - chr2_4:55875127-55875146 Msa0336460:five_prime_UTR 20.0%
!! AACTAATGTATTGAAATACA+AGG + chr2_4:55875376-55875395 None:intergenic 20.0%
! AAATCAGTGTAATTACTTCA+AGG - chr2_4:55873649-55873668 Msa0336460:three_prime_UTR 25.0%
! ATTCTATCTTTAACAGACTA+AGG - chr2_4:55874148-55874167 Msa0336460:CDS 25.0%
!! TTTTAAATCAAGTCCTTGAT+TGG + chr2_4:55874399-55874418 None:intergenic 25.0%
!! AACCGTTTATACAATTTTAC+TGG - chr2_4:55874642-55874661 Msa0336460:CDS 25.0%
!!! GTAATTTTTATTTCCTGGTT+TGG - chr2_4:55875132-55875151 Msa0336460:five_prime_UTR 25.0%
!!! ATATATTATTGACTCTGCTT+TGG + chr2_4:55875228-55875247 None:intergenic 25.0%
! GTCAATAATATATGTGTAGA+AGG - chr2_4:55875235-55875254 Msa0336460:five_prime_UTR 25.0%
! ATAATATATGTGTAGAAGGT+TGG - chr2_4:55875239-55875258 Msa0336460:five_prime_UTR 25.0%
! AAATCAGTGTAATTACTTCA+AGG - chr2_4:55873649-55873668 Msa0336460:three_prime_UTR 25.0%
! ATTCTATCTTTAACAGACTA+AGG - chr2_4:55874148-55874167 Msa0336460:CDS 25.0%
!! TTTTAAATCAAGTCCTTGAT+TGG + chr2_4:55874399-55874418 None:intergenic 25.0%
!! AACCGTTTATACAATTTTAC+TGG - chr2_4:55874642-55874661 Msa0336460:CDS 25.0%
!!! GTAATTTTTATTTCCTGGTT+TGG - chr2_4:55875132-55875151 Msa0336460:five_prime_UTR 25.0%
!!! ATATATTATTGACTCTGCTT+TGG + chr2_4:55875228-55875247 None:intergenic 25.0%
! GTCAATAATATATGTGTAGA+AGG - chr2_4:55875235-55875254 Msa0336460:five_prime_UTR 25.0%
! ATAATATATGTGTAGAAGGT+TGG - chr2_4:55875239-55875258 Msa0336460:five_prime_UTR 25.0%
! ACTTTCATGATTGCTTTGTT+AGG - chr2_4:55873828-55873847 Msa0336460:three_prime_UTR 30.0%
! CTTTCATGATTGCTTTGTTA+GGG - chr2_4:55873829-55873848 Msa0336460:three_prime_UTR 30.0%
!!! ATTTTGATTTAGGGATGTGA+TGG - chr2_4:55873937-55873956 Msa0336460:three_prime_UTR 30.0%
ATAGAATTGAGTACTTACTG+TGG + chr2_4:55874135-55874154 None:intergenic 30.0%
TTAAATAAGCCAATCCAAAC+AGG + chr2_4:55874174-55874193 None:intergenic 30.0%
AGCTTATCTACTGATACATT+TGG - chr2_4:55874238-55874257 Msa0336460:CDS 30.0%
! ATTTGGTGATTTGTGATTTC+AGG - chr2_4:55874255-55874274 Msa0336460:CDS 30.0%
! TTTGGTGATTTGTGATTTCA+GGG - chr2_4:55874256-55874275 Msa0336460:CDS 30.0%
! TTTTCCGTCTCTAATTATGT+CGG + chr2_4:55874517-55874536 None:intergenic 30.0%
GTTTGAAAATGATGTGCAAA+GGG + chr2_4:55874625-55874644 None:intergenic 30.0%
CAATACTACAATAGTCGAAA+TGG - chr2_4:55874743-55874762 Msa0336460:intron 30.0%
TGCTGAATTTGCAAAATCAA+TGG - chr2_4:55874917-55874936 Msa0336460:five_prime_UTR 30.0%
TGCAAAATCAATGGAGAAAA+TGG - chr2_4:55874926-55874945 Msa0336460:five_prime_UTR 30.0%
GCAAAATCAATGGAGAAAAT+GGG - chr2_4:55874927-55874946 Msa0336460:five_prime_UTR 30.0%
!!! TTGATTTGTGTTTTTGGGTT+TGG - chr2_4:55875030-55875049 Msa0336460:intron 30.0%
!!! ATTTTTATTTCCTGGTTTGG+TGG - chr2_4:55875135-55875154 Msa0336460:five_prime_UTR 30.0%
!! GAGAGAAAATTTCTAGTGTT+AGG - chr2_4:55875195-55875214 Msa0336460:five_prime_UTR 30.0%
GAAATACAAGGATCCATTTA+AGG + chr2_4:55875364-55875383 None:intergenic 30.0%
! ACTTTCATGATTGCTTTGTT+AGG - chr2_4:55873828-55873847 Msa0336460:three_prime_UTR 30.0%
! CTTTCATGATTGCTTTGTTA+GGG - chr2_4:55873829-55873848 Msa0336460:three_prime_UTR 30.0%
!!! ATTTTGATTTAGGGATGTGA+TGG - chr2_4:55873937-55873956 Msa0336460:three_prime_UTR 30.0%
ATAGAATTGAGTACTTACTG+TGG + chr2_4:55874135-55874154 None:intergenic 30.0%
TTAAATAAGCCAATCCAAAC+AGG + chr2_4:55874174-55874193 None:intergenic 30.0%
AGCTTATCTACTGATACATT+TGG - chr2_4:55874238-55874257 Msa0336460:CDS 30.0%
! ATTTGGTGATTTGTGATTTC+AGG - chr2_4:55874255-55874274 Msa0336460:CDS 30.0%
! TTTGGTGATTTGTGATTTCA+GGG - chr2_4:55874256-55874275 Msa0336460:CDS 30.0%
! TTTTCCGTCTCTAATTATGT+CGG + chr2_4:55874517-55874536 None:intergenic 30.0%
GTTTGAAAATGATGTGCAAA+GGG + chr2_4:55874625-55874644 None:intergenic 30.0%
CAATACTACAATAGTCGAAA+TGG - chr2_4:55874743-55874762 Msa0336460:intron 30.0%
TGCTGAATTTGCAAAATCAA+TGG - chr2_4:55874917-55874936 Msa0336460:five_prime_UTR 30.0%
TGCAAAATCAATGGAGAAAA+TGG - chr2_4:55874926-55874945 Msa0336460:five_prime_UTR 30.0%
GCAAAATCAATGGAGAAAAT+GGG - chr2_4:55874927-55874946 Msa0336460:five_prime_UTR 30.0%
!!! TTGATTTGTGTTTTTGGGTT+TGG - chr2_4:55875030-55875049 Msa0336460:intron 30.0%
!!! ATTTTTATTTCCTGGTTTGG+TGG - chr2_4:55875135-55875154 Msa0336460:five_prime_UTR 30.0%
!! GAGAGAAAATTTCTAGTGTT+AGG - chr2_4:55875195-55875214 Msa0336460:five_prime_UTR 30.0%
GAAATACAAGGATCCATTTA+AGG + chr2_4:55875364-55875383 None:intergenic 30.0%
!! GCAATGATTAGTTCAGCTTT+TGG + chr2_4:55873746-55873765 None:intergenic 35.0%
!! TGTTGGTTTGTTGAGTTCAA+AGG + chr2_4:55873969-55873988 None:intergenic 35.0%
!!! GAGCATTTTTTTCAGCTTGT+TGG + chr2_4:55873986-55874005 None:intergenic 35.0%
AAGCCTCTAACAGTAAGATT+TGG + chr2_4:55874011-55874030 None:intergenic 35.0%
TGAATGTAGTATTACCAGAG+AGG + chr2_4:55874433-55874452 None:intergenic 35.0%
GTTACCGACATAATTAGAGA+CGG - chr2_4:55874510-55874529 Msa0336460:intron 35.0%
GGTTTGAAAATGATGTGCAA+AGG + chr2_4:55874626-55874645 None:intergenic 35.0%
GCATATTCACTATCAAGTGA+TGG + chr2_4:55874684-55874703 None:intergenic 35.0%
ATCAAATGTGTTTCGACTTC+CGG + chr2_4:55874772-55874791 None:intergenic 35.0%
AGTCGAAACACATTTGATCT+TGG - chr2_4:55874774-55874793 Msa0336460:intron 35.0%
CTTGTGAATCAATTCCTTCA+AGG - chr2_4:55874876-55874895 Msa0336460:five_prime_UTR 35.0%
GACAAGTTAAAGTCAAGACA+GGG - chr2_4:55874949-55874968 Msa0336460:five_prime_UTR 35.0%
TATTGAGACACAACATGACA+TGG + chr2_4:55875069-55875088 None:intergenic 35.0%
! TATATGTGTAGAAGGTTGGT+TGG - chr2_4:55875243-55875262 Msa0336460:five_prime_UTR 35.0%
! AAGGTTGGTTGGTTACATTA+GGG - chr2_4:55875254-55875273 Msa0336460:five_prime_UTR 35.0%
ATGAGAAGTTCTCTAAACCA+AGG - chr2_4:55875333-55875352 Msa0336460:five_prime_UTR 35.0%
!! GCAATGATTAGTTCAGCTTT+TGG + chr2_4:55873746-55873765 None:intergenic 35.0%
!! TGTTGGTTTGTTGAGTTCAA+AGG + chr2_4:55873969-55873988 None:intergenic 35.0%
!!! GAGCATTTTTTTCAGCTTGT+TGG + chr2_4:55873986-55874005 None:intergenic 35.0%
AAGCCTCTAACAGTAAGATT+TGG + chr2_4:55874011-55874030 None:intergenic 35.0%
TGAATGTAGTATTACCAGAG+AGG + chr2_4:55874433-55874452 None:intergenic 35.0%
GTTACCGACATAATTAGAGA+CGG - chr2_4:55874510-55874529 Msa0336460:intron 35.0%
GGTTTGAAAATGATGTGCAA+AGG + chr2_4:55874626-55874645 None:intergenic 35.0%
GCATATTCACTATCAAGTGA+TGG + chr2_4:55874684-55874703 None:intergenic 35.0%
ATCAAATGTGTTTCGACTTC+CGG + chr2_4:55874772-55874791 None:intergenic 35.0%
AGTCGAAACACATTTGATCT+TGG - chr2_4:55874774-55874793 Msa0336460:intron 35.0%
CTTGTGAATCAATTCCTTCA+AGG - chr2_4:55874876-55874895 Msa0336460:five_prime_UTR 35.0%
GACAAGTTAAAGTCAAGACA+GGG - chr2_4:55874949-55874968 Msa0336460:five_prime_UTR 35.0%
TATTGAGACACAACATGACA+TGG + chr2_4:55875069-55875088 None:intergenic 35.0%
! TATATGTGTAGAAGGTTGGT+TGG - chr2_4:55875243-55875262 Msa0336460:five_prime_UTR 35.0%
! AAGGTTGGTTGGTTACATTA+GGG - chr2_4:55875254-55875273 Msa0336460:five_prime_UTR 35.0%
ATGAGAAGTTCTCTAAACCA+AGG - chr2_4:55875333-55875352 Msa0336460:five_prime_UTR 35.0%
! TTCAGCTTTTGGACAACTCT+TGG + chr2_4:55873735-55873754 None:intergenic 40.0%
ATTAATGCAGCAGCAAGTGA+TGG + chr2_4:55873800-55873819 None:intergenic 40.0%
CCTCTAACAGTAAGATTTGG+AGG + chr2_4:55874008-55874027 None:intergenic 40.0%
CCTCCAAATCTTACTGTTAG+AGG - chr2_4:55874005-55874024 Msa0336460:CDS 40.0%
TTAACAGACTAAGGCCTGTT+TGG - chr2_4:55874157-55874176 Msa0336460:CDS 40.0%
CCTGTTGGAACTTTCCAATA+TGG + chr2_4:55874286-55874305 None:intergenic 40.0%
CCATATTGGAAAGTTCCAAC+AGG - chr2_4:55874283-55874302 Msa0336460:CDS 40.0%
GAAAGTTCCAACAGGTAGAA+GGG - chr2_4:55874291-55874310 Msa0336460:CDS 40.0%
TGGAGTCGTATCGAATTTGT+TGG - chr2_4:55874315-55874334 Msa0336460:CDS 40.0%
!!! TGAAGTTTTGGAAATGGAGC+AGG + chr2_4:55874358-55874377 None:intergenic 40.0%
ATTGGCAAAGAGAGTTTGGA+GGG + chr2_4:55874381-55874400 None:intergenic 40.0%
CTTGATTGGCAAAGAGAGTT+TGG + chr2_4:55874385-55874404 None:intergenic 40.0%
CAAACTCTCTTTGCCAATCA+AGG - chr2_4:55874383-55874402 Msa0336460:intron 40.0%
ATGTAGTATTACCAGAGAGG+AGG + chr2_4:55874430-55874449 None:intergenic 40.0%
! GACGACGGTGCATAATTTTA+AGG + chr2_4:55874463-55874482 None:intergenic 40.0%
!!! TTTTCAGGTGCTCACACAAT+TGG - chr2_4:55874594-55874613 Msa0336460:CDS 40.0%
TCAAATGTGTTTCGACTTCC+GGG + chr2_4:55874771-55874790 None:intergenic 40.0%
! AGTCAAGTTGTCAAGAGAAG+AGG - chr2_4:55874804-55874823 Msa0336460:intron 40.0%
! AAGTTTCAAGTGAGCCTTGA+AGG + chr2_4:55874893-55874912 None:intergenic 40.0%
GGACAAGTTAAAGTCAAGAC+AGG - chr2_4:55874948-55874967 Msa0336460:five_prime_UTR 40.0%
ACATAATGTACCACCAAACC+AGG + chr2_4:55875148-55875167 None:intergenic 40.0%
! GAAGGTTGGTTGGTTACATT+AGG - chr2_4:55875253-55875272 Msa0336460:five_prime_UTR 40.0%
AACCAAGGTGCATCCTTAAA+TGG - chr2_4:55875348-55875367 Msa0336460:five_prime_UTR 40.0%
ATCCATTTAAGGATGCACCT+TGG + chr2_4:55875353-55875372 None:intergenic 40.0%
! TTCAGCTTTTGGACAACTCT+TGG + chr2_4:55873735-55873754 None:intergenic 40.0%
ATTAATGCAGCAGCAAGTGA+TGG + chr2_4:55873800-55873819 None:intergenic 40.0%
CCTCTAACAGTAAGATTTGG+AGG + chr2_4:55874008-55874027 None:intergenic 40.0%
CCTCCAAATCTTACTGTTAG+AGG - chr2_4:55874005-55874024 Msa0336460:CDS 40.0%
TTAACAGACTAAGGCCTGTT+TGG - chr2_4:55874157-55874176 Msa0336460:CDS 40.0%
CCTGTTGGAACTTTCCAATA+TGG + chr2_4:55874286-55874305 None:intergenic 40.0%
CCATATTGGAAAGTTCCAAC+AGG - chr2_4:55874283-55874302 Msa0336460:CDS 40.0%
GAAAGTTCCAACAGGTAGAA+GGG - chr2_4:55874291-55874310 Msa0336460:CDS 40.0%
TGGAGTCGTATCGAATTTGT+TGG - chr2_4:55874315-55874334 Msa0336460:CDS 40.0%
!!! TGAAGTTTTGGAAATGGAGC+AGG + chr2_4:55874358-55874377 None:intergenic 40.0%
ATTGGCAAAGAGAGTTTGGA+GGG + chr2_4:55874381-55874400 None:intergenic 40.0%
CTTGATTGGCAAAGAGAGTT+TGG + chr2_4:55874385-55874404 None:intergenic 40.0%
CAAACTCTCTTTGCCAATCA+AGG - chr2_4:55874383-55874402 Msa0336460:intron 40.0%
ATGTAGTATTACCAGAGAGG+AGG + chr2_4:55874430-55874449 None:intergenic 40.0%
! GACGACGGTGCATAATTTTA+AGG + chr2_4:55874463-55874482 None:intergenic 40.0%
!!! TTTTCAGGTGCTCACACAAT+TGG - chr2_4:55874594-55874613 Msa0336460:CDS 40.0%
TCAAATGTGTTTCGACTTCC+GGG + chr2_4:55874771-55874790 None:intergenic 40.0%
! AGTCAAGTTGTCAAGAGAAG+AGG - chr2_4:55874804-55874823 Msa0336460:intron 40.0%
! AAGTTTCAAGTGAGCCTTGA+AGG + chr2_4:55874893-55874912 None:intergenic 40.0%
GGACAAGTTAAAGTCAAGAC+AGG - chr2_4:55874948-55874967 Msa0336460:five_prime_UTR 40.0%
ACATAATGTACCACCAAACC+AGG + chr2_4:55875148-55875167 None:intergenic 40.0%
! GAAGGTTGGTTGGTTACATT+AGG - chr2_4:55875253-55875272 Msa0336460:five_prime_UTR 40.0%
AACCAAGGTGCATCCTTAAA+TGG - chr2_4:55875348-55875367 Msa0336460:five_prime_UTR 40.0%
ATCCATTTAAGGATGCACCT+TGG + chr2_4:55875353-55875372 None:intergenic 40.0%
ACTCATGCTCAATTGCAGCT+AGG - chr2_4:55873701-55873720 Msa0336460:three_prime_UTR 45.0%
CAGCACAAGAAACAACACCA+GGG + chr2_4:55874076-55874095 None:intergenic 45.0%
TCAGCACAAGAAACAACACC+AGG + chr2_4:55874077-55874096 None:intergenic 45.0%
AGACTAAGGCCTGTTTGGAT+TGG - chr2_4:55874162-55874181 Msa0336460:CDS 45.0%
! GGTGATTTGTGATTTCAGGG+TGG - chr2_4:55874259-55874278 Msa0336460:CDS 45.0%
GATTTCAGGGTGGTCCATAT+TGG - chr2_4:55874269-55874288 Msa0336460:CDS 45.0%
GGAAAGTTCCAACAGGTAGA+AGG - chr2_4:55874290-55874309 Msa0336460:CDS 45.0%
!!! GGAGGGTGAAGTTTTGGAAA+TGG + chr2_4:55874364-55874383 None:intergenic 45.0%
! GAGTTTGGAGGGTGAAGTTT+TGG + chr2_4:55874370-55874389 None:intergenic 45.0%
GATTGGCAAAGAGAGTTTGG+AGG + chr2_4:55874382-55874401 None:intergenic 45.0%
AAAGACTTAGTCCTCCTCTC+TGG - chr2_4:55874416-55874435 Msa0336460:intron 45.0%
ACAATAGTCGAAATGGACCC+CGG - chr2_4:55874750-55874769 Msa0336460:intron 45.0%
CAAATGTGTTTCGACTTCCG+GGG + chr2_4:55874770-55874789 None:intergenic 45.0%
AAAGTCAAGACAGGGTCACA+AGG - chr2_4:55874957-55874976 Msa0336460:five_prime_UTR 45.0%
ACTCATGCTCAATTGCAGCT+AGG - chr2_4:55873701-55873720 Msa0336460:three_prime_UTR 45.0%
CAGCACAAGAAACAACACCA+GGG + chr2_4:55874076-55874095 None:intergenic 45.0%
TCAGCACAAGAAACAACACC+AGG + chr2_4:55874077-55874096 None:intergenic 45.0%
AGACTAAGGCCTGTTTGGAT+TGG - chr2_4:55874162-55874181 Msa0336460:CDS 45.0%
! GGTGATTTGTGATTTCAGGG+TGG - chr2_4:55874259-55874278 Msa0336460:CDS 45.0%
GATTTCAGGGTGGTCCATAT+TGG - chr2_4:55874269-55874288 Msa0336460:CDS 45.0%
GGAAAGTTCCAACAGGTAGA+AGG - chr2_4:55874290-55874309 Msa0336460:CDS 45.0%
!!! GGAGGGTGAAGTTTTGGAAA+TGG + chr2_4:55874364-55874383 None:intergenic 45.0%
! GAGTTTGGAGGGTGAAGTTT+TGG + chr2_4:55874370-55874389 None:intergenic 45.0%
GATTGGCAAAGAGAGTTTGG+AGG + chr2_4:55874382-55874401 None:intergenic 45.0%
AAAGACTTAGTCCTCCTCTC+TGG - chr2_4:55874416-55874435 Msa0336460:intron 45.0%
ACAATAGTCGAAATGGACCC+CGG - chr2_4:55874750-55874769 Msa0336460:intron 45.0%
CAAATGTGTTTCGACTTCCG+GGG + chr2_4:55874770-55874789 None:intergenic 45.0%
AAAGTCAAGACAGGGTCACA+AGG - chr2_4:55874957-55874976 Msa0336460:five_prime_UTR 45.0%
!! CAGCAAGTGATGGAGCATTG+TGG + chr2_4:55873790-55873809 None:intergenic 50.0%
! CAGGGCATTCAGCTTCAACA+AGG + chr2_4:55874058-55874077 None:intergenic 50.0%
CTTGTTGAAGCTGAATGCCC+TGG - chr2_4:55874056-55874075 Msa0336460:CDS 50.0%
TGTGGCTGCAATAGAGTCTC+TGG + chr2_4:55874117-55874136 None:intergenic 50.0%
GTTCCAACAGGTAGAAGGGA+TGG - chr2_4:55874295-55874314 Msa0336460:CDS 50.0%
ACTCCATCCCTTCTACCTGT+TGG + chr2_4:55874301-55874320 None:intergenic 50.0%
TGAATTAGCGTGCACGACGA+CGG + chr2_4:55874478-55874497 None:intergenic 50.0%
CAGGGTCACAAGGTGTGATT+AGG - chr2_4:55874967-55874986 Msa0336460:five_prime_UTR 50.0%
!! CAGCAAGTGATGGAGCATTG+TGG + chr2_4:55873790-55873809 None:intergenic 50.0%
! CAGGGCATTCAGCTTCAACA+AGG + chr2_4:55874058-55874077 None:intergenic 50.0%
CTTGTTGAAGCTGAATGCCC+TGG - chr2_4:55874056-55874075 Msa0336460:CDS 50.0%
TGTGGCTGCAATAGAGTCTC+TGG + chr2_4:55874117-55874136 None:intergenic 50.0%
GTTCCAACAGGTAGAAGGGA+TGG - chr2_4:55874295-55874314 Msa0336460:CDS 50.0%
ACTCCATCCCTTCTACCTGT+TGG + chr2_4:55874301-55874320 None:intergenic 50.0%
TGAATTAGCGTGCACGACGA+CGG + chr2_4:55874478-55874497 None:intergenic 50.0%
CAGGGTCACAAGGTGTGATT+AGG - chr2_4:55874967-55874986 Msa0336460:five_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 55873572 55875403 55873572 ID=Msa0336460;Name=Msa0336460
chr2_4 mRNA 55873572 55875403 55873572 ID=Msa0336460-mRNA-1;Parent=Msa0336460;Name=Msa0336460-mRNA-1;_AED=0.07;_eAED=0.07;_QI=504|1|1|1|0.66|0.25|4|387|179
chr2_4 exon 55873572 55874374 55873572 ID=Msa0336460-mRNA-1:exon:16992;Parent=Msa0336460-mRNA-1
chr2_4 exon 55874538 55874698 55874538 ID=Msa0336460-mRNA-1:exon:16991;Parent=Msa0336460-mRNA-1
chr2_4 exon 55874839 55875026 55874839 ID=Msa0336460-mRNA-1:exon:16990;Parent=Msa0336460-mRNA-1
chr2_4 exon 55875125 55875403 55875125 ID=Msa0336460-mRNA-1:exon:16989;Parent=Msa0336460-mRNA-1
chr2_4 five_prime_UTR 55875125 55875403 55875125 ID=Msa0336460-mRNA-1:five_prime_utr;Parent=Msa0336460-mRNA-1
chr2_4 five_prime_UTR 55874839 55875026 55874839 ID=Msa0336460-mRNA-1:five_prime_utr;Parent=Msa0336460-mRNA-1
chr2_4 five_prime_UTR 55874662 55874698 55874662 ID=Msa0336460-mRNA-1:five_prime_utr;Parent=Msa0336460-mRNA-1
chr2_4 CDS 55874538 55874661 55874538 ID=Msa0336460-mRNA-1:cds;Parent=Msa0336460-mRNA-1
chr2_4 CDS 55873959 55874374 55873959 ID=Msa0336460-mRNA-1:cds;Parent=Msa0336460-mRNA-1
chr2_4 three_prime_UTR 55873572 55873958 55873572 ID=Msa0336460-mRNA-1:three_prime_utr;Parent=Msa0336460-mRNA-1
Gene Sequence

>Msa0336460

ATGGAGTCGTATCGAATTTGTTGGAAGCAAATCAAAACATTCCTGCTCCATTTCCAAAACTTCACCCTCCAAACTCTCTTTGCCAATCAAGGACTTGATTTAAAAGACTTAGTCCTCCTCTCTGGTGCTCACACAATTGGTATCTCCCTTTGCACATCATTTTCAAACCGTTTATACAATTTTACTGGAAAGGGTGATCAAGATCCATCACTTGATAGTGAATATGCACAAAATCTAAAGACTTTCAAGTGCAAGAACATCAATGACAATACTACAATAGTCGAAATGGACCCCGGAAGTCGAAACACATTTGATCTTGGTTACTATAGTCAAGTTGTCAAGAGAAGAGGTTTGTTTCAGTCTGATTCTGCATTGTTGACAAATTCAGTAACAAAAGCTCTTGTGAATCAATTCCTTCAAGGCTCACTTGAAACTTTCTATGCTGAATTTGCAAAATCAATGGAGAAAATGGGACAAGTTAAAGTCAAGACAGGGTCACAAGGTGTGATTAGGAAGCATTGTGCACTTGTAAATAACTAA
Protein sequence

>Msa0336460

MESYRICWKQIKTFLLHFQNFTLQTLFANQGLDLKDLVLLSGAHTIGISLCTSFSNRLYNFTGKGDQDPSLDSEYAQNLKTFKCKNINDNTTIVEMDPGSRNTFDLGYYSQVVKRRGLFQSDSALLTNSVTKALVNQFLQGSLETFYAEFAKSMEKMGQVKVKTGSQGVIRKHCALVNN*