AlfalfaGEDB | Msa0663640

M. sativa cultivar zhongmu-4 / Msa0663640

KEGG: -
GO: -
PFAM: PF00583.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0663640	XP_003604253.1	96.333	409	14	1	1	408	1	409	0.0	818

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0663640	sp\|Q42381\|HLS1_ARATH	48.775	408	179	7	24	408	3	403	3.32e-125	370

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0663640	G7JEG0	96.333	409	14	1	1	408	1	409	0.0	818

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TFs/TRs:

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Gene ID	Type	Classification
Msa0663640	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0538110	Msa0663640	0.927229	1.572115e-91	-8.615850e-47
Msa0585780	Msa0663640	0.913514	5.632506e-84	-8.615850e-47
Msa0663260	Msa0663640	0.805546	1.266773e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0663640	MtrunA17_Chr4g0001411	96.333	409	14	1	1	408	1	409	0.0	818
Msa0663640	MtrunA17_Chr4g0053961	48.551	414	176	9	23	408	7	411	1.27e-124	366
Msa0663640	MtrunA17_Chr5g0401351	47.277	404	186	7	23	408	9	403	1.79e-121	358
Msa0663640	MtrunA17_Chr3g0105631	47.395	403	183	13	18	407	1	387	3.19e-108	323
Msa0663640	MtrunA17_Chr4g0068331	35.381	407	238	10	20	408	8	407	7.07e-68	220

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0663640	AT4G37580.1	48.775	408	179	7	24	408	3	403	3.37e-126	370
Msa0663640	AT2G23060.1	46.875	416	184	7	23	408	5	413	6.04e-125	367
Msa0663640	AT5G67430.1	43.250	400	190	10	23	407	8	385	2.40e-104	314
Msa0663640	AT4G37580.2	46.571	350	158	6	81	408	4	346	1.07e-101	306
Msa0663640	AT5G67430.2	42.388	335	163	8	88	407	1	320	1.06e-84	261
Msa0663640	AT2G23060.2	44.406	286	144	5	131	408	1	279	4.27e-81	251
Msa0663640	AT2G30090.1	39.695	393	205	11	25	407	15	385	5.85e-79	249

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Find 86 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAAATCTATGTCTCT+TGG	0.388928	4_4:-2557228	None:intergenic
AAAATTGGAAAGGCTTTGTG+AGG	0.581130	4_4:+2555380	Msa0663640:CDS
AAATCAAATCTATGTCTCTT+GGG	0.426207	4_4:-2557227	None:intergenic
AAGATAATTTCCTCCAATGT+GGG	0.570772	4_4:-2557659	None:intergenic
AAGTGACGAATTCTGCATAT+AGG	0.481918	4_4:-2555450	None:intergenic
AATAGGTCAGTAACAAGAGA+AGG	0.604941	4_4:-2555420	None:intergenic
AATATGGCTAGAGATGATGG+TGG	0.797279	4_4:+2557582	Msa0663640:CDS
AATGAAGGTAACCTTAGCCA+TGG	0.631481	4_4:-2557456	None:intergenic
AATGTATGGATTACACATGG+AGG	0.692644	4_4:+2557512	Msa0663640:CDS
AATGTGAGTACTCAAAATTC+AGG	0.364355	4_4:+2557073	Msa0663640:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTTCAATTACATGTTATGC+TGG	+	chr4_4:2555470-2555489	Msa0663640:CDS	30.0%
GCATTAGGTATACATCAATA+TGG	-	chr4_4:2555727-2555746	None:intergenic	30.0%
CTAAATCATAATGTCGCATT+AGG	-	chr4_4:2555742-2555761	None:intergenic	30.0%
AGTATTTATGACTCAAGACA+CGG	-	chr4_4:2555839-2555858	None:intergenic	30.0%
CTAGTTTCACAGTTTGATAT+TGG	+	chr4_4:2555860-2555879	Msa0663640:intron	30.0%
TGAAAATAGAACCTGTGTTT+GGG	-	chr4_4:2556036-2556055	None:intergenic	30.0%
TTGAAAATAGAACCTGTGTT+TGG	-	chr4_4:2556037-2556056	None:intergenic	30.0%
CAACTTTCTCATACTTCATT+TGG	+	chr4_4:2556514-2556533	Msa0663640:intron	30.0%
TATTGTCTAGATGAAGAACT+TGG	-	chr4_4:2556542-2556561	None:intergenic	30.0%
AGTTCTTCATCTAGACAATA+AGG	+	chr4_4:2556542-2556561	Msa0663640:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	2555267	2557818	2555267	ID=Msa0663640;Name=Msa0663640
chr4_4	mRNA	2555267	2557818	2555267	ID=Msa0663640-mRNA-1;Parent=Msa0663640;Name=Msa0663640-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|0\|0\|1\|1\|1\|3\|0\|408
chr4_4	exon	2555267	2555491	2555267	ID=Msa0663640-mRNA-1:exon:677;Parent=Msa0663640-mRNA-1
chr4_4	exon	2555913	2556043	2555913	ID=Msa0663640-mRNA-1:exon:678;Parent=Msa0663640-mRNA-1
chr4_4	exon	2556948	2557818	2556948	ID=Msa0663640-mRNA-1:exon:679;Parent=Msa0663640-mRNA-1
chr4_4	CDS	2555267	2555491	2555267	ID=Msa0663640-mRNA-1:cds;Parent=Msa0663640-mRNA-1
chr4_4	CDS	2555913	2556043	2555913	ID=Msa0663640-mRNA-1:cds;Parent=Msa0663640-mRNA-1
chr4_4	CDS	2556948	2557818	2556948	ID=Msa0663640-mRNA-1:cds;Parent=Msa0663640-mRNA-1

Gene Sequence

>Msa0663640

ATGTCTTTGAAGATAGCAGCTGAAACTTGGCAAAAACCATCATCAAAGAAGGTTGAAGAAGCAAAGATAGTGATAAGAAGTTATGATGAAGAGAAACACAAAGTGGCAGTAGAAAAATTGGAAAGGCTTTGTGAGGTTGGACAAAGAGGAAAACCTTCTCTTGTTACTGACCTATTGGGTGATCCTATATGCAGAATTCGTCACTTTCAATTACATGTTATGCTGGTTGCAGAATATGGAGAAGAAGGAGAAGTTGTAGGAGTGATAAGGGGTTGTGTGAAAACAGTTACAAGAGGGAATTCAGCTTATGTAAAACTTGCTTATGTTTTAGGACTCAGAGTCTCTCCCAAACACAGGAGATTTGGCATTGGCACAAAATTAGTTGAACATTTAGAAGAGTGGTGCAAACAAAAAGGGGCTAAGTATGCATATATGGCAACTGATTGTACAAATGAGCCATCAATCAATTTGTTCACAAAAAAATGTGAGTACTCAAAATTCAGGACTCTAACAATGCTAGTCCAGCCTGTTCATGCTCACTACAAGCCAATCAGCACAAACATTGCTGTCCTTCGCCTACCATCGCGCTTGGCCGGGCCGACATACAACCATATGTTTGCCAATGCAGAATTTTTCCCAAGAGACATAGATTTGATTTTGTCAAACAAACTTAATTTGGGCACATTTATGGCCATACCTAAAAAAGACCTAACAAAATGTGATCCAAAAAATGGTATTTTCCCTCCAAGCTATGCAATATTAAGTGTGTGGAACACAAAAGAAGTGTTCAAATTGCAAGTTAAAGGAGCTTCACCATTTGTACATGCATGTTGTGTAGGTACAAGATTATTAGATGAATGTATGCCATGGCTAAGGTTACCTTCATTTCCTAATGTTTTTAGGCCATTTGGGATTTATCTAATGTATGGATTACACATGGAGGGTAAATGTGGAAAACAACTTATGAAGTCATTATGTGGATTTGTACATAATATGGCTAGAGATGATGGTGGTTGTGGGGCCATTGTGACTGAAGTGAGTCAAAGGGACCCAGTTAGAGAAGTGATCCCACATTGGAGGAAATTATCTTGGGCTGAGGACATGTGGTGCATTAAGAGTCTTGAGCACATGAAGAAGGATAGTATACATGAAAAATGTGGACCCTCAGATTGGTACAATTATAGATCCTCTTCATCAGTAATTTTTGTTGATCCTCGTGATTTTTAA

Protein sequence

>Msa0663640

MSLKIAAETWQKPSSKKVEEAKIVIRSYDEEKHKVAVEKLERLCEVGQRGKPSLVTDLLGDPICRIRHFQLHVMLVAEYGEEGEVVGVIRGCVKTVTRGNSAYVKLAYVLGLRVSPKHRRFGIGTKLVEHLEEWCKQKGAKYAYMATDCTNEPSINLFTKKCEYSKFRTLTMLVQPVHAHYKPISTNIAVLRLPSRLAGPTYNHMFANAEFFPRDIDLILSNKLNLGTFMAIPKKDLTKCDPKNGIFPPSYAILSVWNTKEVFKLQVKGASPFVHACCVGTRLLDECMPWLRLPSFPNVFRPFGIYLMYGLHMEGKCGKQLMKSLCGFVHNMARDDGGCGAIVTEVSQRDPVREVIPHWRKLSWAEDMWCIKSLEHMKKDSIHEKCGPSDWYNYRSSSSVIFVDPRDF*