AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0665510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0665510 MtrunA17_Chr4g0003581 96.629 89 3 0 1 89 1 89 1.34e-60 183
Msa0665510 MtrunA17_Chr2g0332851 79.545 88 18 0 1 88 1 88 1.60e-48 152
Msa0665510 MtrunA17_Chr2g0332841 66.364 110 31 2 1 109 1 105 8.39e-45 142
Msa0665510 MtrunA17_Chr4g0063501 65.882 85 29 0 5 89 1 85 1.25e-40 132
Msa0665510 MtrunA17_Chr2g0277151 61.728 81 31 0 8 88 2 82 3.53e-32 111
Msa0665510 MtrunA17_Chr5g0405171 55.056 89 32 2 6 88 22 108 9.10e-29 103
Msa0665510 MtrunA17_Chr8g0372141 55.294 85 34 1 8 88 12 96 1.54e-27 99.8
Msa0665510 MtrunA17_Chr4g0020201 51.648 91 39 2 2 89 29 117 1.98e-27 99.8
Msa0665510 MtrunA17_Chr1g0212241 67.692 65 21 0 24 88 1 65 3.29e-27 97.4
Msa0665510 MtrunA17_Chr4g0070901 34.483 87 55 1 13 97 72 158 6.41e-12 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0665510 AT1G80160.1 77.528 89 20 0 1 89 1 89 1.05e-48 153
Msa0665510 AT1G80160.3 78.409 88 19 0 1 88 42 129 2.16e-48 153
Msa0665510 AT1G15380.2 71.910 89 25 0 1 89 1 89 4.69e-45 144
Msa0665510 AT1G15380.1 71.910 89 25 0 1 89 1 89 4.69e-45 144
Msa0665510 AT2G28420.1 51.765 85 40 1 11 94 18 102 2.25e-28 102
Msa0665510 AT1G80160.2 72.222 36 10 0 53 88 27 62 7.10e-12 58.5
Msa0665510 AT5G57040.1 32.692 104 61 2 14 108 79 182 3.33e-11 57.8

Find 69 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTAACATCTTTGTTATT+TGG 0.109561 4_4:+5093067 Msa0665510:five_prime_UTR
ATTGATTACGTTTCAAAATT+TGG 0.126932 4_4:+5093773 Msa0665510:CDS
ATCCAACTTTCTTAATTTAA+TGG 0.127326 4_4:+5094270 Msa0665510:three_prime_UTR
TTAATAAAGTAATGAATTAT+TGG 0.150400 4_4:+5094949 Msa0665510:three_prime_UTR
AAAGAACCAGGCCTTCTTAT+TGG 0.200598 4_4:-5093270 None:intergenic
TCGAAACAGAGTGACTTATT+TGG 0.221146 4_4:+5094986 Msa0665510:three_prime_UTR
TTCTTCCTTGGTAACGTCTC+TGG 0.223323 4_4:-5093686 None:intergenic
GACCTAATTTGCAGGCTATT+TGG 0.231443 4_4:+5093626 Msa0665510:intron
AATTTCTACCAGAATGTTCT+TGG 0.254660 4_4:+5093240 Msa0665510:CDS
GCCATTGCATATCCTTAAAA+AGG 0.260168 4_4:-5093987 None:intergenic
TTTAAGGATCTTATCCTCTA+AGG 0.285109 4_4:+5094011 Msa0665510:three_prime_UTR
CTTATTTGGACTGATATTAT+AGG 0.285486 4_4:+5095000 Msa0665510:three_prime_UTR
TTATTAATAATTAACATATA+AGG 0.298592 4_4:-5094933 None:intergenic
GGATTTATTTCTTTCTTCCT+TGG 0.309354 4_4:-5093698 None:intergenic
AAGAACCAGGCCTTCTTATT+GGG 0.313845 4_4:-5093269 None:intergenic
GCAATTTGAAGCAACCTTAG+AGG 0.323527 4_4:-5094025 None:intergenic
TTGTTAACCATTCGATTCTC+AGG 0.349832 4_4:+5094345 Msa0665510:three_prime_UTR
AGGCTATTTGGCTATGGTAT+TGG 0.365518 4_4:+5093638 Msa0665510:intron
TACCATTAAATTAAGAAAGT+TGG 0.369024 4_4:-5094272 None:intergenic
ATCTCAATCATGAAACCATC+TGG 0.372159 4_4:-5094659 None:intergenic
CTTGCTACCTCACCAACTAA+TGG 0.386113 4_4:-5094710 None:intergenic
AAAGATATGTGATTATCCTT+TGG 0.393062 4_4:-5093719 None:intergenic
GGAAGAAACCAAGAACATTC+TGG 0.404879 4_4:-5093248 None:intergenic
GGATAATCACATATCTTTCC+AGG 0.407468 4_4:+5093724 Msa0665510:CDS
CTTTCATAGTTCAATTGGGT+TGG 0.410235 4_4:+5093103 Msa0665510:five_prime_UTR
GGAAAAGAGCTCTGGTAATT+CGG 0.412673 4_4:+5094367 Msa0665510:three_prime_UTR
CTTCCTGTGATTCCATTAGT+TGG 0.419159 4_4:+5094698 Msa0665510:three_prime_UTR
TCTTCCTTGGTAACGTCTCT+GGG 0.440829 4_4:-5093685 None:intergenic
GCATAATATATGCAGAGGTC+GGG 0.442119 4_4:+5094169 Msa0665510:three_prime_UTR
AAACATCTTTGTGTGGGAAA+AGG 0.452378 4_4:+5094825 Msa0665510:three_prime_UTR
CGATTCACAAGTTATTGTTA+AGG 0.463839 4_4:-5093001 None:intergenic
TTGGTTTCTTCCCAATAAGA+AGG 0.469291 4_4:+5093259 Msa0665510:CDS
ATTTGCAGGCTATTTGGCTA+TGG 0.473328 4_4:+5093632 Msa0665510:intron
TTCTTCCCAATAAGAAGGCC+TGG 0.475428 4_4:+5093264 Msa0665510:CDS
TGTTAAGGAAGAGAAGAAAG+AGG 0.482716 4_4:-5092986 None:intergenic
TGCATAATATATGCAGAGGT+CGG 0.491908 4_4:+5094168 Msa0665510:three_prime_UTR
GCATCGTGCAATTGTAGTAA+AGG 0.495935 4_4:+5094297 Msa0665510:three_prime_UTR
TCATGAAACCATCTGGGTCA+TGG 0.503506 4_4:-5094652 None:intergenic
ATTCTCAGGGAAAAGAGCTC+TGG 0.513593 4_4:+5094359 Msa0665510:three_prime_UTR
TGAGAAAATGAAGGAAAGTG+TGG 0.520447 4_4:+5093149 Msa0665510:exon
GTGAGCTTAACTCGGTTGGT+AGG 0.521211 4_4:+5094140 Msa0665510:three_prime_UTR
AGAGGAAGGTGGGATTGAAG+TGG 0.523433 4_4:+5094616 Msa0665510:three_prime_UTR
TACTGCAGTGTGAGAGCATG+GGG 0.524607 4_4:+5094540 Msa0665510:intron
TGAGCTTAACTCGGTTGGTA+GGG 0.529442 4_4:+5094141 Msa0665510:three_prime_UTR
CTATGTGAGCTTAACTCGGT+TGG 0.537863 4_4:+5094136 Msa0665510:three_prime_UTR
TCACCAACTAATGGAATCAC+AGG 0.547180 4_4:-5094701 None:intergenic
AGTACTGCAGTGTGAGAGCA+TGG 0.548571 4_4:+5094538 Msa0665510:intron
TGTGATTCCATTAGTTGGTG+AGG 0.549148 4_4:+5094703 Msa0665510:three_prime_UTR
GTACTGCAGTGTGAGAGCAT+GGG 0.563693 4_4:+5094539 Msa0665510:intron
CATAATATATGCAGAGGTCG+GGG 0.565323 4_4:+5094170 Msa0665510:three_prime_UTR
TGTTAACCATTCGATTCTCA+GGG 0.568057 4_4:+5094346 Msa0665510:three_prime_UTR
TAGTCTATGTGAGCTTAACT+CGG 0.575311 4_4:+5094132 Msa0665510:three_prime_UTR
GAGCAAGAGTAGAGGAAGGT+GGG 0.575892 4_4:+5094606 Msa0665510:three_prime_UTR
GAGAAAATGAAGGAAAGTGT+GGG 0.578884 4_4:+5093150 Msa0665510:exon
CGAGCAAGAGTAGAGGAAGG+TGG 0.581051 4_4:+5094605 Msa0665510:three_prime_UTR
GAAGAAAGAAATAAATCCAA+AGG 0.593095 4_4:+5093703 Msa0665510:CDS
CCATCAAAATCAAAAGAACC+AGG 0.594472 4_4:-5093282 None:intergenic
TAAGTCACTCTGTTTCGAGT+AGG 0.607940 4_4:-5094981 None:intergenic
TCTCAATCATGAAACCATCT+GGG 0.612866 4_4:-5094658 None:intergenic
TAAACGTGTCTATGATGACT+AGG 0.619283 4_4:-5093958 None:intergenic
AATGAATAGAAGATTTGTCA+CGG 0.633905 4_4:-5092950 None:intergenic
TTATGTTCGAGCAAGAGTAG+AGG 0.637893 4_4:+5094598 Msa0665510:three_prime_UTR
AGAACCCAGAGACGTTACCA+AGG 0.641794 4_4:+5093681 Msa0665510:CDS
GTTCGAGCAAGAGTAGAGGA+AGG 0.649580 4_4:+5094602 Msa0665510:three_prime_UTR
ACAATGCATAATATATGCAG+AGG 0.654813 4_4:+5094164 Msa0665510:three_prime_UTR
TAATTCGGAGTTCGACCGTG+AGG 0.657158 4_4:+5094382 Msa0665510:three_prime_UTR
TGTGACCAAACATCTTTGTG+TGG 0.679161 4_4:+5094818 Msa0665510:three_prime_UTR
GTGACCAAACATCTTTGTGT+GGG 0.690684 4_4:+5094819 Msa0665510:three_prime_UTR
ACTGCAGTGTGAGAGCATGG+GGG 0.779396 4_4:+5094541 Msa0665510:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAATTTTTTTGTTAAAA+CGG + chr4_4:5093390-5093409 Msa0665510:intron 10.0%
!!! CAAAAATTATTAAATTTTGA+GGG - chr4_4:5093545-5093564 None:intergenic 10.0%
!!! ACAAAAATTATTAAATTTTG+AGG - chr4_4:5093546-5093565 None:intergenic 10.0%
!!! TCTATATTTAGTTTTAAATT+TGG - chr4_4:5093909-5093928 None:intergenic 10.0%
!!! GTAATATATTTTTTTTATCA+AGG - chr4_4:5094249-5094268 None:intergenic 10.0%
!! TTAATAAAGTAATGAATTAT+TGG + chr4_4:5094949-5094968 Msa0665510:three_prime_UTR 10.0%
!!! TTTCAATTTTTTTGTTAAAA+CGG + chr4_4:5093390-5093409 Msa0665510:intron 10.0%
!!! CAAAAATTATTAAATTTTGA+GGG - chr4_4:5093545-5093564 None:intergenic 10.0%
!!! ACAAAAATTATTAAATTTTG+AGG - chr4_4:5093546-5093565 None:intergenic 10.0%
!!! TCTATATTTAGTTTTAAATT+TGG - chr4_4:5093909-5093928 None:intergenic 10.0%
!!! GTAATATATTTTTTTTATCA+AGG - chr4_4:5094249-5094268 None:intergenic 10.0%
!! TTAATAAAGTAATGAATTAT+TGG + chr4_4:5094949-5094968 Msa0665510:three_prime_UTR 10.0%
!!! AATTTAACATCTTTGTTATT+TGG + chr4_4:5093067-5093086 Msa0665510:five_prime_UTR 15.0%
!! ACACAAAATTTCATATTAAA+TGG - chr4_4:5094493-5094512 None:intergenic 15.0%
!!! AATTTAACATCTTTGTTATT+TGG + chr4_4:5093067-5093086 Msa0665510:five_prime_UTR 15.0%
!! ACACAAAATTTCATATTAAA+TGG - chr4_4:5094493-5094512 None:intergenic 15.0%
!!! GATGTTAAATTTTAGAGAAA+GGG - chr4_4:5093058-5093077 None:intergenic 20.0%
!!! AGATGTTAAATTTTAGAGAA+AGG - chr4_4:5093059-5093078 None:intergenic 20.0%
!! TTGAACTATGAAAGAAAAAA+CGG - chr4_4:5093097-5093116 None:intergenic 20.0%
!!! TTTTTCTTTCATAGTTCAAT+TGG + chr4_4:5093098-5093117 Msa0665510:five_prime_UTR 20.0%
!!! TTTTCTTTCATAGTTCAATT+GGG + chr4_4:5093099-5093118 Msa0665510:five_prime_UTR 20.0%
!!! TAATTTTCTTGAGAAAATGA+AGG + chr4_4:5093140-5093159 Msa0665510:exon 20.0%
!! TCAATTGAAAACAACTTAAA+AGG + chr4_4:5093495-5093514 Msa0665510:intron 20.0%
!! CAATTGAAAACAACTTAAAA+GGG + chr4_4:5093496-5093515 Msa0665510:intron 20.0%
!! AATTGAAAACAACTTAAAAG+GGG + chr4_4:5093497-5093516 Msa0665510:intron 20.0%
!!! ATTTTGAAACGTAATCAATA+AGG - chr4_4:5093772-5093791 None:intergenic 20.0%
!! ATTGATTACGTTTCAAAATT+TGG + chr4_4:5093773-5093792 Msa0665510:CDS 20.0%
!!! GAGTGTTTTAGTTTTAAATT+TGG - chr4_4:5093937-5093956 None:intergenic 20.0%
!! ATCCAACTTTCTTAATTTAA+TGG + chr4_4:5094270-5094289 Msa0665510:three_prime_UTR 20.0%
!! TACCATTAAATTAAGAAAGT+TGG - chr4_4:5094275-5094294 None:intergenic 20.0%
!!! GATGTTAAATTTTAGAGAAA+GGG - chr4_4:5093058-5093077 None:intergenic 20.0%
!!! AGATGTTAAATTTTAGAGAA+AGG - chr4_4:5093059-5093078 None:intergenic 20.0%
!! TTGAACTATGAAAGAAAAAA+CGG - chr4_4:5093097-5093116 None:intergenic 20.0%
!!! TTTTTCTTTCATAGTTCAAT+TGG + chr4_4:5093098-5093117 Msa0665510:five_prime_UTR 20.0%
!!! TTTTCTTTCATAGTTCAATT+GGG + chr4_4:5093099-5093118 Msa0665510:five_prime_UTR 20.0%
!!! TAATTTTCTTGAGAAAATGA+AGG + chr4_4:5093140-5093159 Msa0665510:exon 20.0%
!! TCAATTGAAAACAACTTAAA+AGG + chr4_4:5093495-5093514 Msa0665510:intron 20.0%
!! CAATTGAAAACAACTTAAAA+GGG + chr4_4:5093496-5093515 Msa0665510:intron 20.0%
!! AATTGAAAACAACTTAAAAG+GGG + chr4_4:5093497-5093516 Msa0665510:intron 20.0%
!!! ATTTTGAAACGTAATCAATA+AGG - chr4_4:5093772-5093791 None:intergenic 20.0%
!! ATTGATTACGTTTCAAAATT+TGG + chr4_4:5093773-5093792 Msa0665510:CDS 20.0%
!!! GAGTGTTTTAGTTTTAAATT+TGG - chr4_4:5093937-5093956 None:intergenic 20.0%
!! ATCCAACTTTCTTAATTTAA+TGG + chr4_4:5094270-5094289 Msa0665510:three_prime_UTR 20.0%
!! TACCATTAAATTAAGAAAGT+TGG - chr4_4:5094275-5094294 None:intergenic 20.0%
! AATGAATAGAAGATTTGTCA+CGG - chr4_4:5092953-5092972 None:intergenic 25.0%
!!! AGATATGATTCACTGATTTT+AGG - chr4_4:5093188-5093207 None:intergenic 25.0%
!! GAATTTAATGAACTTTTGCT+TGG + chr4_4:5093570-5093589 Msa0665510:intron 25.0%
!!! TGAAAATTGATTTTTGCATG+TGG + chr4_4:5093602-5093621 Msa0665510:intron 25.0%
! GAAGAAAGAAATAAATCCAA+AGG + chr4_4:5093703-5093722 Msa0665510:CDS 25.0%
! AAAGATATGTGATTATCCTT+TGG - chr4_4:5093722-5093741 None:intergenic 25.0%
!! CGTTTATGTTATCCTTTTTA+AGG + chr4_4:5093975-5093994 Msa0665510:three_prime_UTR 25.0%
! GTGCAAAAAATTACATGATT+CGG - chr4_4:5094065-5094084 None:intergenic 25.0%
! GTTTCATCAGAAATTAAACT+TGG + chr4_4:5094428-5094447 Msa0665510:intron 25.0%
!! CTCAACAATTTGTCATTTAT+CGG + chr4_4:5094457-5094476 Msa0665510:intron 25.0%
! TTTGTGTTTGAACTTTGAAT+TGG + chr4_4:5094506-5094525 Msa0665510:intron 25.0%
! TGAGAAAAAGCTAAAAGAAA+TGG + chr4_4:5094568-5094587 Msa0665510:three_prime_UTR 25.0%
! CTTATTTGGACTGATATTAT+AGG + chr4_4:5095000-5095019 Msa0665510:three_prime_UTR 25.0%
! AATGAATAGAAGATTTGTCA+CGG - chr4_4:5092953-5092972 None:intergenic 25.0%
!!! AGATATGATTCACTGATTTT+AGG - chr4_4:5093188-5093207 None:intergenic 25.0%
!! GAATTTAATGAACTTTTGCT+TGG + chr4_4:5093570-5093589 Msa0665510:intron 25.0%
!!! TGAAAATTGATTTTTGCATG+TGG + chr4_4:5093602-5093621 Msa0665510:intron 25.0%
! GAAGAAAGAAATAAATCCAA+AGG + chr4_4:5093703-5093722 Msa0665510:CDS 25.0%
! AAAGATATGTGATTATCCTT+TGG - chr4_4:5093722-5093741 None:intergenic 25.0%
!! CGTTTATGTTATCCTTTTTA+AGG + chr4_4:5093975-5093994 Msa0665510:three_prime_UTR 25.0%
! GTGCAAAAAATTACATGATT+CGG - chr4_4:5094065-5094084 None:intergenic 25.0%
! GTTTCATCAGAAATTAAACT+TGG + chr4_4:5094428-5094447 Msa0665510:intron 25.0%
!! CTCAACAATTTGTCATTTAT+CGG + chr4_4:5094457-5094476 Msa0665510:intron 25.0%
! TTTGTGTTTGAACTTTGAAT+TGG + chr4_4:5094506-5094525 Msa0665510:intron 25.0%
! TGAGAAAAAGCTAAAAGAAA+TGG + chr4_4:5094568-5094587 Msa0665510:three_prime_UTR 25.0%
! CTTATTTGGACTGATATTAT+AGG + chr4_4:5095000-5095019 Msa0665510:three_prime_UTR 25.0%
CGATTCACAAGTTATTGTTA+AGG - chr4_4:5093004-5093023 None:intergenic 30.0%
AATTTCTACCAGAATGTTCT+TGG + chr4_4:5093240-5093259 Msa0665510:CDS 30.0%
!!! CTGGTTCTTTTGATTTTGAT+GGG + chr4_4:5093283-5093302 Msa0665510:CDS 30.0%
!!! TGGTTCTTTTGATTTTGATG+GGG + chr4_4:5093284-5093303 Msa0665510:CDS 30.0%
! GGATTTATTTCTTTCTTCCT+TGG - chr4_4:5093701-5093720 None:intergenic 30.0%
!!! TTTTTCTATGCGTCACTTTT+AGG - chr4_4:5093885-5093904 None:intergenic 30.0%
!! TCCTTTTTAAGGATATGCAA+TGG + chr4_4:5093986-5094005 Msa0665510:three_prime_UTR 30.0%
TTTAAGGATCTTATCCTCTA+AGG + chr4_4:5094011-5094030 Msa0665510:three_prime_UTR 30.0%
!!! ATGTAATTTTTTGCACTGCA+CGG + chr4_4:5094069-5094088 Msa0665510:three_prime_UTR 30.0%
ACAATGCATAATATATGCAG+AGG + chr4_4:5094164-5094183 Msa0665510:three_prime_UTR 30.0%
!!! TTTTTTATCAAGGAGACTAG+TGG - chr4_4:5094239-5094258 None:intergenic 30.0%
CGATAAATGACAAATTGTTG+AGG - chr4_4:5094459-5094478 None:intergenic 30.0%
CGATTCACAAGTTATTGTTA+AGG - chr4_4:5093004-5093023 None:intergenic 30.0%
AATTTCTACCAGAATGTTCT+TGG + chr4_4:5093240-5093259 Msa0665510:CDS 30.0%
!!! CTGGTTCTTTTGATTTTGAT+GGG + chr4_4:5093283-5093302 Msa0665510:CDS 30.0%
!!! TGGTTCTTTTGATTTTGATG+GGG + chr4_4:5093284-5093303 Msa0665510:CDS 30.0%
! GGATTTATTTCTTTCTTCCT+TGG - chr4_4:5093701-5093720 None:intergenic 30.0%
!!! TTTTTCTATGCGTCACTTTT+AGG - chr4_4:5093885-5093904 None:intergenic 30.0%
!! TCCTTTTTAAGGATATGCAA+TGG + chr4_4:5093986-5094005 Msa0665510:three_prime_UTR 30.0%
TTTAAGGATCTTATCCTCTA+AGG + chr4_4:5094011-5094030 Msa0665510:three_prime_UTR 30.0%
!!! ATGTAATTTTTTGCACTGCA+CGG + chr4_4:5094069-5094088 Msa0665510:three_prime_UTR 30.0%
ACAATGCATAATATATGCAG+AGG + chr4_4:5094164-5094183 Msa0665510:three_prime_UTR 30.0%
!!! TTTTTTATCAAGGAGACTAG+TGG - chr4_4:5094239-5094258 None:intergenic 30.0%
CGATAAATGACAAATTGTTG+AGG - chr4_4:5094459-5094478 None:intergenic 30.0%
TGTTAAGGAAGAGAAGAAAG+AGG - chr4_4:5092989-5093008 None:intergenic 35.0%
CTTTCATAGTTCAATTGGGT+TGG + chr4_4:5093103-5093122 Msa0665510:five_prime_UTR 35.0%
TGAGAAAATGAAGGAAAGTG+TGG + chr4_4:5093149-5093168 Msa0665510:exon 35.0%
! GAGAAAATGAAGGAAAGTGT+GGG + chr4_4:5093150-5093169 Msa0665510:exon 35.0%
!!! TCACTGATTTTAGGTGCAAA+GGG - chr4_4:5093179-5093198 None:intergenic 35.0%
!!! TTCACTGATTTTAGGTGCAA+AGG - chr4_4:5093180-5093199 None:intergenic 35.0%
TTGGTTTCTTCCCAATAAGA+AGG + chr4_4:5093259-5093278 Msa0665510:CDS 35.0%
CCATCAAAATCAAAAGAACC+AGG - chr4_4:5093285-5093304 None:intergenic 35.0%
!!! CCTGGTTCTTTTGATTTTGA+TGG + chr4_4:5093282-5093301 Msa0665510:CDS 35.0%
GCCATTGTTAGTATTATGCT+GGG + chr4_4:5093412-5093431 Msa0665510:intron 35.0%
TCCCAGCATAATACTAACAA+TGG - chr4_4:5093416-5093435 None:intergenic 35.0%
GGATAATCACATATCTTTCC+AGG + chr4_4:5093724-5093743 Msa0665510:CDS 35.0%
GTTTAGTGAAAAATCTGACC+TGG - chr4_4:5093745-5093764 None:intergenic 35.0%
TAAACGTGTCTATGATGACT+AGG - chr4_4:5093961-5093980 None:intergenic 35.0%
GCCATTGCATATCCTTAAAA+AGG - chr4_4:5093990-5094009 None:intergenic 35.0%
! AGGATATGCAATGGCTTTTA+AGG + chr4_4:5093995-5094014 Msa0665510:three_prime_UTR 35.0%
TAGTCTATGTGAGCTTAACT+CGG + chr4_4:5094132-5094151 Msa0665510:three_prime_UTR 35.0%
TGCATAATATATGCAGAGGT+CGG + chr4_4:5094168-5094187 Msa0665510:three_prime_UTR 35.0%
!! CATTTTAAATACGAGGTGTC+TGG - chr4_4:5094204-5094223 None:intergenic 35.0%
! GCTCACACATTTTAAATACG+AGG - chr4_4:5094211-5094230 None:intergenic 35.0%
TTGTTAACCATTCGATTCTC+AGG + chr4_4:5094345-5094364 Msa0665510:three_prime_UTR 35.0%
TGTTAACCATTCGATTCTCA+GGG + chr4_4:5094346-5094365 Msa0665510:three_prime_UTR 35.0%
ATCAGACTATACTTACCTCA+CGG - chr4_4:5094400-5094419 None:intergenic 35.0%
TCTCAATCATGAAACCATCT+GGG - chr4_4:5094661-5094680 None:intergenic 35.0%
ATCTCAATCATGAAACCATC+TGG - chr4_4:5094662-5094681 None:intergenic 35.0%
! TTTTCCCACACAAAGATGTT+TGG - chr4_4:5094826-5094845 None:intergenic 35.0%
AAACATCTTTGTGTGGGAAA+AGG + chr4_4:5094825-5094844 Msa0665510:three_prime_UTR 35.0%
TCGAAACAGAGTGACTTATT+TGG + chr4_4:5094986-5095005 Msa0665510:three_prime_UTR 35.0%
TGTTAAGGAAGAGAAGAAAG+AGG - chr4_4:5092989-5093008 None:intergenic 35.0%
CTTTCATAGTTCAATTGGGT+TGG + chr4_4:5093103-5093122 Msa0665510:five_prime_UTR 35.0%
TGAGAAAATGAAGGAAAGTG+TGG + chr4_4:5093149-5093168 Msa0665510:exon 35.0%
! GAGAAAATGAAGGAAAGTGT+GGG + chr4_4:5093150-5093169 Msa0665510:exon 35.0%
!!! TCACTGATTTTAGGTGCAAA+GGG - chr4_4:5093179-5093198 None:intergenic 35.0%
!!! TTCACTGATTTTAGGTGCAA+AGG - chr4_4:5093180-5093199 None:intergenic 35.0%
TTGGTTTCTTCCCAATAAGA+AGG + chr4_4:5093259-5093278 Msa0665510:CDS 35.0%
CCATCAAAATCAAAAGAACC+AGG - chr4_4:5093285-5093304 None:intergenic 35.0%
!!! CCTGGTTCTTTTGATTTTGA+TGG + chr4_4:5093282-5093301 Msa0665510:CDS 35.0%
GCCATTGTTAGTATTATGCT+GGG + chr4_4:5093412-5093431 Msa0665510:intron 35.0%
TCCCAGCATAATACTAACAA+TGG - chr4_4:5093416-5093435 None:intergenic 35.0%
GGATAATCACATATCTTTCC+AGG + chr4_4:5093724-5093743 Msa0665510:CDS 35.0%
GTTTAGTGAAAAATCTGACC+TGG - chr4_4:5093745-5093764 None:intergenic 35.0%
TAAACGTGTCTATGATGACT+AGG - chr4_4:5093961-5093980 None:intergenic 35.0%
GCCATTGCATATCCTTAAAA+AGG - chr4_4:5093990-5094009 None:intergenic 35.0%
! AGGATATGCAATGGCTTTTA+AGG + chr4_4:5093995-5094014 Msa0665510:three_prime_UTR 35.0%
TAGTCTATGTGAGCTTAACT+CGG + chr4_4:5094132-5094151 Msa0665510:three_prime_UTR 35.0%
TGCATAATATATGCAGAGGT+CGG + chr4_4:5094168-5094187 Msa0665510:three_prime_UTR 35.0%
!! CATTTTAAATACGAGGTGTC+TGG - chr4_4:5094204-5094223 None:intergenic 35.0%
! GCTCACACATTTTAAATACG+AGG - chr4_4:5094211-5094230 None:intergenic 35.0%
TTGTTAACCATTCGATTCTC+AGG + chr4_4:5094345-5094364 Msa0665510:three_prime_UTR 35.0%
TGTTAACCATTCGATTCTCA+GGG + chr4_4:5094346-5094365 Msa0665510:three_prime_UTR 35.0%
ATCAGACTATACTTACCTCA+CGG - chr4_4:5094400-5094419 None:intergenic 35.0%
TCTCAATCATGAAACCATCT+GGG - chr4_4:5094661-5094680 None:intergenic 35.0%
ATCTCAATCATGAAACCATC+TGG - chr4_4:5094662-5094681 None:intergenic 35.0%
! TTTTCCCACACAAAGATGTT+TGG - chr4_4:5094826-5094845 None:intergenic 35.0%
AAACATCTTTGTGTGGGAAA+AGG + chr4_4:5094825-5094844 Msa0665510:three_prime_UTR 35.0%
TCGAAACAGAGTGACTTATT+TGG + chr4_4:5094986-5095005 Msa0665510:three_prime_UTR 35.0%
GGAAGAAACCAAGAACATTC+TGG - chr4_4:5093251-5093270 None:intergenic 40.0%
AAGAACCAGGCCTTCTTATT+GGG - chr4_4:5093272-5093291 None:intergenic 40.0%
AAAGAACCAGGCCTTCTTAT+TGG - chr4_4:5093273-5093292 None:intergenic 40.0%
!!! CTTTTGATTTTGATGGGGCA+TGG + chr4_4:5093289-5093308 Msa0665510:CDS 40.0%
GGCCATTGTTAGTATTATGC+TGG + chr4_4:5093411-5093430 Msa0665510:intron 40.0%
! GACCTAATTTGCAGGCTATT+TGG + chr4_4:5093626-5093645 Msa0665510:intron 40.0%
AGCCAAATAGCCTGCAAATT+AGG - chr4_4:5093631-5093650 None:intergenic 40.0%
ATTTGCAGGCTATTTGGCTA+TGG + chr4_4:5093632-5093651 Msa0665510:intron 40.0%
AGGCTATTTGGCTATGGTAT+TGG + chr4_4:5093638-5093657 Msa0665510:intron 40.0%
! GCAATTTGAAGCAACCTTAG+AGG - chr4_4:5094028-5094047 None:intergenic 40.0%
GCATAATATATGCAGAGGTC+GGG + chr4_4:5094169-5094188 Msa0665510:three_prime_UTR 40.0%
CATAATATATGCAGAGGTCG+GGG + chr4_4:5094170-5094189 Msa0665510:three_prime_UTR 40.0%
GCATCGTGCAATTGTAGTAA+AGG + chr4_4:5094297-5094316 Msa0665510:three_prime_UTR 40.0%
! TCTTTTCCCTGAGAATCGAA+TGG - chr4_4:5094355-5094374 None:intergenic 40.0%
! GGAAAAGAGCTCTGGTAATT+CGG + chr4_4:5094367-5094386 Msa0665510:three_prime_UTR 40.0%
TTATGTTCGAGCAAGAGTAG+AGG + chr4_4:5094598-5094617 Msa0665510:three_prime_UTR 40.0%
!! CTATTTTTCCATGACCCAGA+TGG + chr4_4:5094644-5094663 Msa0665510:three_prime_UTR 40.0%
CTTCCTGTGATTCCATTAGT+TGG + chr4_4:5094698-5094717 Msa0665510:three_prime_UTR 40.0%
TCACCAACTAATGGAATCAC+AGG - chr4_4:5094704-5094723 None:intergenic 40.0%
! TGTGATTCCATTAGTTGGTG+AGG + chr4_4:5094703-5094722 Msa0665510:three_prime_UTR 40.0%
TGTGACCAAACATCTTTGTG+TGG + chr4_4:5094818-5094837 Msa0665510:three_prime_UTR 40.0%
GTGACCAAACATCTTTGTGT+GGG + chr4_4:5094819-5094838 Msa0665510:three_prime_UTR 40.0%
! TAAGTCACTCTGTTTCGAGT+AGG - chr4_4:5094984-5095003 None:intergenic 40.0%
GGAAGAAACCAAGAACATTC+TGG - chr4_4:5093251-5093270 None:intergenic 40.0%
AAGAACCAGGCCTTCTTATT+GGG - chr4_4:5093272-5093291 None:intergenic 40.0%
AAAGAACCAGGCCTTCTTAT+TGG - chr4_4:5093273-5093292 None:intergenic 40.0%
!!! CTTTTGATTTTGATGGGGCA+TGG + chr4_4:5093289-5093308 Msa0665510:CDS 40.0%
GGCCATTGTTAGTATTATGC+TGG + chr4_4:5093411-5093430 Msa0665510:intron 40.0%
! GACCTAATTTGCAGGCTATT+TGG + chr4_4:5093626-5093645 Msa0665510:intron 40.0%
AGCCAAATAGCCTGCAAATT+AGG - chr4_4:5093631-5093650 None:intergenic 40.0%
ATTTGCAGGCTATTTGGCTA+TGG + chr4_4:5093632-5093651 Msa0665510:intron 40.0%
AGGCTATTTGGCTATGGTAT+TGG + chr4_4:5093638-5093657 Msa0665510:intron 40.0%
! GCAATTTGAAGCAACCTTAG+AGG - chr4_4:5094028-5094047 None:intergenic 40.0%
GCATAATATATGCAGAGGTC+GGG + chr4_4:5094169-5094188 Msa0665510:three_prime_UTR 40.0%
CATAATATATGCAGAGGTCG+GGG + chr4_4:5094170-5094189 Msa0665510:three_prime_UTR 40.0%
GCATCGTGCAATTGTAGTAA+AGG + chr4_4:5094297-5094316 Msa0665510:three_prime_UTR 40.0%
! TCTTTTCCCTGAGAATCGAA+TGG - chr4_4:5094355-5094374 None:intergenic 40.0%
! GGAAAAGAGCTCTGGTAATT+CGG + chr4_4:5094367-5094386 Msa0665510:three_prime_UTR 40.0%
TTATGTTCGAGCAAGAGTAG+AGG + chr4_4:5094598-5094617 Msa0665510:three_prime_UTR 40.0%
!! CTATTTTTCCATGACCCAGA+TGG + chr4_4:5094644-5094663 Msa0665510:three_prime_UTR 40.0%
CTTCCTGTGATTCCATTAGT+TGG + chr4_4:5094698-5094717 Msa0665510:three_prime_UTR 40.0%
TCACCAACTAATGGAATCAC+AGG - chr4_4:5094704-5094723 None:intergenic 40.0%
! TGTGATTCCATTAGTTGGTG+AGG + chr4_4:5094703-5094722 Msa0665510:three_prime_UTR 40.0%
TGTGACCAAACATCTTTGTG+TGG + chr4_4:5094818-5094837 Msa0665510:three_prime_UTR 40.0%
GTGACCAAACATCTTTGTGT+GGG + chr4_4:5094819-5094838 Msa0665510:three_prime_UTR 40.0%
! TAAGTCACTCTGTTTCGAGT+AGG - chr4_4:5094984-5095003 None:intergenic 40.0%
TTCTTCCCAATAAGAAGGCC+TGG + chr4_4:5093264-5093283 Msa0665510:CDS 45.0%
! CATGTGGTGACCTAATTTGC+AGG + chr4_4:5093618-5093637 Msa0665510:intron 45.0%
!! TCTTCCTTGGTAACGTCTCT+GGG - chr4_4:5093688-5093707 None:intergenic 45.0%
!! TTCTTCCTTGGTAACGTCTC+TGG - chr4_4:5093689-5093708 None:intergenic 45.0%
CTATGTGAGCTTAACTCGGT+TGG + chr4_4:5094136-5094155 Msa0665510:three_prime_UTR 45.0%
! TGAGCTTAACTCGGTTGGTA+GGG + chr4_4:5094141-5094160 Msa0665510:three_prime_UTR 45.0%
ATTCTCAGGGAAAAGAGCTC+TGG + chr4_4:5094359-5094378 Msa0665510:three_prime_UTR 45.0%
TCATGAAACCATCTGGGTCA+TGG - chr4_4:5094655-5094674 None:intergenic 45.0%
CTTGCTACCTCACCAACTAA+TGG - chr4_4:5094713-5094732 None:intergenic 45.0%
TTCTTCCCAATAAGAAGGCC+TGG + chr4_4:5093264-5093283 Msa0665510:CDS 45.0%
! CATGTGGTGACCTAATTTGC+AGG + chr4_4:5093618-5093637 Msa0665510:intron 45.0%
!! TCTTCCTTGGTAACGTCTCT+GGG - chr4_4:5093688-5093707 None:intergenic 45.0%
!! TTCTTCCTTGGTAACGTCTC+TGG - chr4_4:5093689-5093708 None:intergenic 45.0%
CTATGTGAGCTTAACTCGGT+TGG + chr4_4:5094136-5094155 Msa0665510:three_prime_UTR 45.0%
! TGAGCTTAACTCGGTTGGTA+GGG + chr4_4:5094141-5094160 Msa0665510:three_prime_UTR 45.0%
ATTCTCAGGGAAAAGAGCTC+TGG + chr4_4:5094359-5094378 Msa0665510:three_prime_UTR 45.0%
TCATGAAACCATCTGGGTCA+TGG - chr4_4:5094655-5094674 None:intergenic 45.0%
CTTGCTACCTCACCAACTAA+TGG - chr4_4:5094713-5094732 None:intergenic 45.0%
!! TTATTAATAATTAACATATA+AGG - chr4_4:5094936-5094955 None:intergenic 5.0%
!! TTATTAATAATTAACATATA+AGG - chr4_4:5094936-5094955 None:intergenic 5.0%
AGAACCCAGAGACGTTACCA+AGG + chr4_4:5093681-5093700 Msa0665510:CDS 50.0%
GTGAGCTTAACTCGGTTGGT+AGG + chr4_4:5094140-5094159 Msa0665510:three_prime_UTR 50.0%
TAATTCGGAGTTCGACCGTG+AGG + chr4_4:5094382-5094401 Msa0665510:three_prime_UTR 50.0%
AGTACTGCAGTGTGAGAGCA+TGG + chr4_4:5094538-5094557 Msa0665510:intron 50.0%
! GTACTGCAGTGTGAGAGCAT+GGG + chr4_4:5094539-5094558 Msa0665510:intron 50.0%
! TACTGCAGTGTGAGAGCATG+GGG + chr4_4:5094540-5094559 Msa0665510:intron 50.0%
GTTCGAGCAAGAGTAGAGGA+AGG + chr4_4:5094602-5094621 Msa0665510:three_prime_UTR 50.0%
GAGCAAGAGTAGAGGAAGGT+GGG + chr4_4:5094606-5094625 Msa0665510:three_prime_UTR 50.0%
AGAGGAAGGTGGGATTGAAG+TGG + chr4_4:5094616-5094635 Msa0665510:three_prime_UTR 50.0%
AGAACCCAGAGACGTTACCA+AGG + chr4_4:5093681-5093700 Msa0665510:CDS 50.0%
GTGAGCTTAACTCGGTTGGT+AGG + chr4_4:5094140-5094159 Msa0665510:three_prime_UTR 50.0%
TAATTCGGAGTTCGACCGTG+AGG + chr4_4:5094382-5094401 Msa0665510:three_prime_UTR 50.0%
AGTACTGCAGTGTGAGAGCA+TGG + chr4_4:5094538-5094557 Msa0665510:intron 50.0%
! GTACTGCAGTGTGAGAGCAT+GGG + chr4_4:5094539-5094558 Msa0665510:intron 50.0%
! TACTGCAGTGTGAGAGCATG+GGG + chr4_4:5094540-5094559 Msa0665510:intron 50.0%
GTTCGAGCAAGAGTAGAGGA+AGG + chr4_4:5094602-5094621 Msa0665510:three_prime_UTR 50.0%
GAGCAAGAGTAGAGGAAGGT+GGG + chr4_4:5094606-5094625 Msa0665510:three_prime_UTR 50.0%
AGAGGAAGGTGGGATTGAAG+TGG + chr4_4:5094616-5094635 Msa0665510:three_prime_UTR 50.0%
! ACTGCAGTGTGAGAGCATGG+GGG + chr4_4:5094541-5094560 Msa0665510:intron 55.0%
CGAGCAAGAGTAGAGGAAGG+TGG + chr4_4:5094605-5094624 Msa0665510:three_prime_UTR 55.0%
! ACTGCAGTGTGAGAGCATGG+GGG + chr4_4:5094541-5094560 Msa0665510:intron 55.0%
CGAGCAAGAGTAGAGGAAGG+TGG + chr4_4:5094605-5094624 Msa0665510:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 5092950 5095024 5092950 ID=Msa0665510;Name=Msa0665510
chr4_4 mRNA 5092950 5095024 5092950 ID=Msa0665510-mRNA-1;Parent=Msa0665510;Name=Msa0665510-mRNA-1;_AED=0.04;_eAED=0.04;_QI=206|1|1|1|1|0.66|3|1066|109
chr4_4 exon 5092950 5093310 5092950 ID=Msa0665510-mRNA-1:exon:1676;Parent=Msa0665510-mRNA-1
chr4_4 exon 5093640 5094403 5093640 ID=Msa0665510-mRNA-1:exon:1677;Parent=Msa0665510-mRNA-1
chr4_4 exon 5094548 5095024 5094548 ID=Msa0665510-mRNA-1:exon:1678;Parent=Msa0665510-mRNA-1
chr4_4 five_prime_UTR 5092950 5093155 5092950 ID=Msa0665510-mRNA-1:five_prime_utr;Parent=Msa0665510-mRNA-1
chr4_4 CDS 5093156 5093310 5093156 ID=Msa0665510-mRNA-1:cds;Parent=Msa0665510-mRNA-1
chr4_4 CDS 5093640 5093814 5093640 ID=Msa0665510-mRNA-1:cds;Parent=Msa0665510-mRNA-1
chr4_4 three_prime_UTR 5093815 5094403 5093815 ID=Msa0665510-mRNA-1:three_prime_utr;Parent=Msa0665510-mRNA-1
chr4_4 three_prime_UTR 5094548 5095024 5094548 ID=Msa0665510-mRNA-1:three_prime_utr;Parent=Msa0665510-mRNA-1
Gene Sequence

>Msa0665510

ATGAAGGAAAGTGTGGGAAACCCTTTGCACCTAAAATCAGTGAATCATATCTCACTCATATGCAGATCAGTTGAAGAGTCAATTAATTTCTACCAGAATGTTCTTGGTTTCTTCCCAATAAGAAGGCCTGGTTCTTTTGATTTTGATGGGGCATGGCTATTTGGCTATGGTATTGGTATTCATTTGCTAGAAGCAGAGAACCCAGAGACGTTACCAAGGAAGAAAGAAATAAATCCAAAGGATAATCACATATCTTTCCAGGTCAGATTTTTCACTAAACTTAACCTTATTGATTACGTTTCAAAATTTGGAAGAAAAAAAATAATGTGA
Protein sequence

>Msa0665510

MKESVGNPLHLKSVNHISLICRSVEESINFYQNVLGFFPIRRPGSFDFDGAWLFGYGIGIHLLEAENPETLPRKKEINPKDNHISFQVRFFTKLNLIDYVSKFGRKKIM*