AlfalfaGEDB | Msa0530700

M. sativa cultivar zhongmu-4 / Msa0530700

KEGG: -
GO: -
PFAM: PF00903.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0530700	XP_013454666.1	86.869	198	1	2	1	196	1	175	1.44e-116	338

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0530700	sp\|Q9XI31\|GLYI4_ARATH	66.667	195	42	1	1	195	1	172	1.63e-91	268

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0530700	A0A072UG80	86.869	198	1	2	1	196	1	175	6.89e-117	338

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0530700	Msa0587530	0.891705	3.112940e-74	-8.615850e-47
Msa0530700	Msa0627770	0.884263	2.226696e-71	-8.615850e-47
Msa0530700	Msa0665510	0.876169	1.728207e-68	-8.615850e-47
Msa0530700	Msa1298100	0.820640	6.163472e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0530700	MtrunA17_Chr4g0003581	86.869	198	1	2	1	196	1	175	1.33e-120	338
Msa0530700	MtrunA17_Chr2g0332851	65.816	196	40	2	1	196	1	169	1.30e-85	249
Msa0530700	MtrunA17_Chr2g0332841	62.434	189	47	2	1	188	1	166	1.07e-77	229
Msa0530700	MtrunA17_Chr4g0063501	57.714	175	50	2	5	178	1	152	7.44e-71	212
Msa0530700	MtrunA17_Chr1g0212241	59.036	166	44	2	24	188	1	143	3.14e-61	187
Msa0530700	MtrunA17_Chr2g0277151	53.459	159	51	1	8	166	2	137	3.97e-56	175
Msa0530700	MtrunA17_Chr5g0405171	47.093	172	60	3	6	171	22	168	1.29e-50	162
Msa0530700	MtrunA17_Chr8g0372141	46.626	163	60	2	8	166	12	151	1.38e-49	159
Msa0530700	MtrunA17_Chr4g0020201	41.837	196	83	5	2	191	29	199	8.29e-48	155

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0530700	AT1G15380.2	66.667	195	42	1	1	195	1	172	1.66e-92	268
Msa0530700	AT1G15380.1	66.667	195	42	1	1	195	1	172	1.66e-92	268
Msa0530700	AT1G80160.1	69.792	192	32	2	1	192	1	166	4.00e-92	266
Msa0530700	AT1G80160.3	69.792	192	32	2	1	192	42	207	6.21e-92	268
Msa0530700	AT1G80160.2	65.000	140	23	2	53	192	27	140	3.05e-55	172
Msa0530700	AT2G28420.1	46.497	157	59	3	11	165	18	151	4.40e-48	155

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Find 53 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATCTTTGTGTGGGAAA+AGG	0.452378	4_1:+4763939	Msa0530700:three_prime_UTR
AAAGAACCAGGCCTTCTTAT+TGG	0.200598	4_1:-4761896	None:intergenic
AAAGATATGTGATTATCCTT+TGG	0.352158	4_1:-4762850	None:intergenic
AAGAACCAGGCCTTCTTATT+GGG	0.313845	4_1:-4761895	None:intergenic
AAGATATGTGATTATCCTTT+GGG	0.325525	4_1:-4762849	None:intergenic
AATGAATAGAAGATTTGTCA+CGG	0.633905	4_1:-4761581	None:intergenic
AATTTAACATCTTTGTTATT+TGG	0.109561	4_1:+4761698	Msa0530700:five_prime_UTR
AATTTCTACCAGAATGTTCT+TGG	0.254660	4_1:+4761866	Msa0530700:CDS
AGAACCCAGAGACGTTACCA+AGG	0.641794	4_1:+4762812	Msa0530700:CDS
AGAAGAAGGTGGGATTGAAG+TGG	0.480570	4_1:+4763730	Msa0530700:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CGATTCACAAGTTATTGTTA+AGG	-	chr4_1:4761635-4761654	None:intergenic	30.0%
AATTTCTACCAGAATGTTCT+TGG	+	chr4_1:4761866-4761885	Msa0530700:CDS	30.0%
TTATAGGGACTAAAAGTTGA+AGG	+	chr4_1:4762428-4762447	Msa0530700:intron	30.0%
CGAAAAGTTGACATATTTAC+AGG	+	chr4_1:4762472-4762491	Msa0530700:intron	30.0%
GAAGAAAGAAATAAACCCAA+AGG	+	chr4_1:4762834-4762853	Msa0530700:CDS	30.0%
TTTAAGGATCTTATCCTCTA+AGG	+	chr4_1:4763141-4763160	Msa0530700:intron	30.0%
GTATAATATATGCAGAAGTC+GGG	+	chr4_1:4763294-4763313	Msa0530700:intron	30.0%
TGAGAAAAAGCTGAAAGAAA+TGG	+	chr4_1:4763682-4763701	Msa0530700:CDS	30.0%
CTTATTTGGACTGCTATTAT+TGG	+	chr4_1:4764114-4764133	Msa0530700:three_prime_UTR	30.0%
TGTTAAGGAAGAGAAGAAAG+AGG	-	chr4_1:4761620-4761639	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	4761581	4764138	4761581	ID=Msa0530700;Name=Msa0530700
chr4_1	mRNA	4761581	4764138	4761581	ID=Msa0530700-mRNA-1;Parent=Msa0530700;Name=Msa0530700-mRNA-1;_AED=0.21;_eAED=0.21;_QI=201\|0.66\|0.75\|1\|1\|1\|4\|216\|196
chr4_1	exon	4761581	4761936	4761581	ID=Msa0530700-mRNA-1:exon:1433;Parent=Msa0530700-mRNA-1
chr4_1	exon	4762771	4762876	4762771	ID=Msa0530700-mRNA-1:exon:1434;Parent=Msa0530700-mRNA-1
chr4_1	exon	4763460	4763528	4763460	ID=Msa0530700-mRNA-1:exon:1435;Parent=Msa0530700-mRNA-1
chr4_1	exon	4763662	4764138	4763662	ID=Msa0530700-mRNA-1:exon:1436;Parent=Msa0530700-mRNA-1
chr4_1	five_prime_UTR	4761581	4761781	4761581	ID=Msa0530700-mRNA-1:five_prime_utr;Parent=Msa0530700-mRNA-1
chr4_1	CDS	4761782	4761936	4761782	ID=Msa0530700-mRNA-1:cds;Parent=Msa0530700-mRNA-1
chr4_1	CDS	4762771	4762876	4762771	ID=Msa0530700-mRNA-1:cds;Parent=Msa0530700-mRNA-1
chr4_1	CDS	4763460	4763528	4763460	ID=Msa0530700-mRNA-1:cds;Parent=Msa0530700-mRNA-1
chr4_1	CDS	4763662	4763922	4763662	ID=Msa0530700-mRNA-1:cds;Parent=Msa0530700-mRNA-1
chr4_1	three_prime_UTR	4763923	4764138	4763923	ID=Msa0530700-mRNA-1:three_prime_utr;Parent=Msa0530700-mRNA-1

Gene Sequence

>Msa0530700

ATGAAGGAAAGTGTGGGAAATCCTTTGCACCTAAAATCAGTGAATCATATCTCACTCATATGCAGATCAGTTGAAGAGTCAATTAATTTCTACCAGAATGTTCTTGGTTTCTTCCCAATAAGAAGGCCTGGTTCTTTTGATTTTGATGGGGCATGGCTATTTGGCTATGGTATTGGTATTCATTTGCTAGAAGCAGAGAACCCAGAGACGTTACCAAGGAAGAAAGAAATAAACCCAAAGGATAATCACATATCTTTCCAGCTTTTTGTTGTTGTTAACCATTCGATTCTCAGGGAAAAGAGCTCTGGTAATTCGGAGTTCGATCTCGAGTGTGAGAGTATGGGGGCAGTTGAGAAAAAGCTGAAAGAAATGGAAATCAATTATGTTCGAGCAAGAGTAGAAGAAGGTGGGATTGAAGTGGATCAGCTATTTTTCCATGACCCAGATGGTTTCATGATTGAGATATGCAATTGTGATAGTCTTCCTGTGATTCCATTAGTTGGTGAGGTAGCAAGATCATGCTCACGATTGAATCTTCACATAATGCAGAATCAGAATCAGCAGAATCAGATACATAAGATTGTGAAGTGA

Protein sequence

>Msa0530700

MKESVGNPLHLKSVNHISLICRSVEESINFYQNVLGFFPIRRPGSFDFDGAWLFGYGIGIHLLEAENPETLPRKKEINPKDNHISFQLFVVVNHSILREKSSGNSEFDLECESMGAVEKKLKEMEINYVRARVEEGGIEVDQLFFHDPDGFMIEICNCDSLPVIPLVGEVARSCSRLNLHIMQNQNQQNQIHKIVK*