AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0587530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0587530 MtrunA17_Chr4g0003581 97.753 89 2 0 1 89 1 89 2.57e-61 184
Msa0587530 MtrunA17_Chr2g0332851 79.545 88 18 0 1 88 1 88 2.19e-48 152
Msa0587530 MtrunA17_Chr2g0332841 67.961 103 28 1 1 103 1 98 1.29e-44 142
Msa0587530 MtrunA17_Chr4g0063501 67.059 85 28 0 5 89 1 85 2.35e-41 134
Msa0587530 MtrunA17_Chr2g0277151 61.728 81 31 0 8 88 2 82 3.76e-32 111
Msa0587530 MtrunA17_Chr5g0405171 55.056 89 32 2 6 88 22 108 1.08e-28 103
Msa0587530 MtrunA17_Chr4g0020201 52.747 91 38 2 2 89 29 117 4.28e-28 101
Msa0587530 MtrunA17_Chr8g0372141 55.294 85 34 1 8 88 12 96 2.08e-27 99.4
Msa0587530 MtrunA17_Chr1g0212241 57.831 83 30 1 24 106 1 78 2.45e-27 97.8
Msa0587530 MtrunA17_Chr4g0070901 35.632 87 54 1 13 97 72 158 1.03e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0587530 AT1G80160.1 77.528 89 20 0 1 89 1 89 1.64e-48 152
Msa0587530 AT1G80160.3 78.409 88 19 0 1 88 42 129 3.53e-48 153
Msa0587530 AT1G15380.2 70.787 89 26 0 1 89 1 89 2.49e-44 142
Msa0587530 AT1G15380.1 70.787 89 26 0 1 89 1 89 2.49e-44 142
Msa0587530 AT2G28420.1 51.765 85 40 1 11 94 18 102 2.04e-28 102
Msa0587530 AT1G80160.2 72.222 36 10 0 53 88 27 62 7.26e-12 58.5
Msa0587530 AT5G57040.1 34.409 93 57 2 14 104 79 169 1.94e-11 58.2

Find 70 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGATTACGTTTCAAAATT+TGG 0.088946 4_2:+9103403 Msa0587530:CDS
AATTTAACATCTTTGTTATT+TGG 0.109561 4_2:+9102726 Msa0587530:five_prime_UTR
ATCCAACTTTCTTAATTTAA+TGG 0.127326 4_2:+9103893 Msa0587530:three_prime_UTR
TTAATAAAGTAATGAATTAT+TGG 0.150400 4_2:+9104564 Msa0587530:three_prime_UTR
AAAGAACCAGGCCTTCTTAT+TGG 0.200598 4_2:-9102929 None:intergenic
TCGAAACAGAGTGACTTATT+TGG 0.221146 4_2:+9104601 Msa0587530:three_prime_UTR
TTCTTCCTTGGTAACGTCTC+TGG 0.223323 4_2:-9103316 None:intergenic
GACCTAATTTGCAGGCTATT+TGG 0.231443 4_2:+9103256 Msa0587530:intron
GCCATTGCATATCCTTAAAA+AGG 0.260168 4_2:-9103612 None:intergenic
GATTTCTACCAGAATGTTCT+TGG 0.277645 4_2:+9102899 Msa0587530:CDS
TTTAAGGATCTTATCCTCTA+AGG 0.285109 4_2:+9103638 Msa0587530:three_prime_UTR
CTTATTTGGACTGCTATTAT+TGG 0.287923 4_2:+9104615 Msa0587530:three_prime_UTR
TTATTAATAATTAACATATA+AGG 0.298592 4_2:-9104548 None:intergenic
GGATTTATTTCTTTCTTCCT+TGG 0.309354 4_2:-9103328 None:intergenic
AAGAACCAGGCCTTCTTATT+GGG 0.313845 4_2:-9102928 None:intergenic
GCAATTTGAAGCAACCTTAG+AGG 0.323527 4_2:-9103652 None:intergenic
TTGTTAACCATTCGATTCTC+AGG 0.349832 4_2:+9103968 Msa0587530:three_prime_UTR
AGGCTATTTGGCTATGGTAT+TGG 0.365518 4_2:+9103268 Msa0587530:intron
TACCATTAAATTAAGAAAGT+TGG 0.369024 4_2:-9103895 None:intergenic
ATCTCAATCATGAAACCATC+TGG 0.372159 4_2:-9104274 None:intergenic
CTTGCTACCTCACCAACTAA+TGG 0.386113 4_2:-9104325 None:intergenic
GTGATCACAAATTATTGTTA+AGG 0.387388 4_2:-9102666 None:intergenic
AAAGATATGTGATTATCCTT+TGG 0.393062 4_2:-9103349 None:intergenic
GGAAGAAACCAAGAACATTC+TGG 0.404879 4_2:-9102907 None:intergenic
GGATAATCACATATCTTTCC+AGG 0.407468 4_2:+9103354 Msa0587530:CDS
CTTTCATAGTTCAATTGGGT+TGG 0.410235 4_2:+9102762 Msa0587530:five_prime_UTR
GGAAAAGAGCTCTGGTAATT+CGG 0.412673 4_2:+9103990 Msa0587530:three_prime_UTR
CTTCCTGTGATTCCATTAGT+TGG 0.419159 4_2:+9104313 Msa0587530:three_prime_UTR
TCTTCCTTGGTAACGTCTCT+GGG 0.440829 4_2:-9103315 None:intergenic
GCATAATATATGCAGAGGTC+GGG 0.442119 4_2:+9103791 Msa0587530:three_prime_UTR
AAACATCTTTGTGTGGGAAA+AGG 0.452378 4_2:+9104440 Msa0587530:three_prime_UTR
TTGGTTTCTTCCCAATAAGA+AGG 0.469291 4_2:+9102918 Msa0587530:CDS
ATTTGCAGGCTATTTGGCTA+TGG 0.473328 4_2:+9103262 Msa0587530:intron
TTCTTCCCAATAAGAAGGCC+TGG 0.475428 4_2:+9102923 Msa0587530:CDS
TGTTAAGGAAGAGAAGAAAG+AGG 0.482716 4_2:-9102651 None:intergenic
TGCATAATATATGCAGAGGT+CGG 0.491908 4_2:+9103790 Msa0587530:three_prime_UTR
GCATCGTGCAATTGTAGTAA+AGG 0.495935 4_2:+9103920 Msa0587530:three_prime_UTR
TGATGTGCAGTGTGAGAGCA+TGG 0.503338 4_2:+9104153 Msa0587530:intron
TCATGAAACCATCTGGGTCA+TGG 0.503506 4_2:-9104267 None:intergenic
GATGTGCAGTGTGAGAGCAT+GGG 0.508852 4_2:+9104154 Msa0587530:intron
ATTCTCAGGGAAAAGAGCTC+TGG 0.513593 4_2:+9103982 Msa0587530:three_prime_UTR
TGAGAAAATGAAGGAAAGTG+TGG 0.520447 4_2:+9102808 Msa0587530:exon
GTGAGCTTAACTCGGTTGGT+AGG 0.521211 4_2:+9103762 Msa0587530:three_prime_UTR
AGAGGAAGGTGGGATTGAAG+TGG 0.523433 4_2:+9104231 Msa0587530:three_prime_UTR
TGAGCTTAACTCGGTTGGTA+GGG 0.529442 4_2:+9103763 Msa0587530:three_prime_UTR
AGACGTACTTCAATAAATGA+AGG 0.535260 4_2:+9103461 Msa0587530:three_prime_UTR
CTATGTGAGCTTAACTCGGT+TGG 0.537863 4_2:+9103758 Msa0587530:three_prime_UTR
TCACCAACTAATGGAATCAC+AGG 0.547180 4_2:-9104316 None:intergenic
TGTGATTCCATTAGTTGGTG+AGG 0.549148 4_2:+9104318 Msa0587530:three_prime_UTR
CATAATATATGCAGAGGTCG+GGG 0.565323 4_2:+9103792 Msa0587530:three_prime_UTR
TGTTAACCATTCGATTCTCA+GGG 0.568057 4_2:+9103969 Msa0587530:three_prime_UTR
TAGTCTATGTGAGCTTAACT+CGG 0.575311 4_2:+9103754 Msa0587530:three_prime_UTR
GAGCAAGAGTAGAGGAAGGT+GGG 0.575892 4_2:+9104221 Msa0587530:three_prime_UTR
GAGAAAATGAAGGAAAGTGT+GGG 0.578884 4_2:+9102809 Msa0587530:exon
CGAGCAAGAGTAGAGGAAGG+TGG 0.581051 4_2:+9104220 Msa0587530:three_prime_UTR
GAAGAAAGAAATAAATCCAA+AGG 0.593095 4_2:+9103333 Msa0587530:CDS
CCATCAAAATCAAAAGAACC+AGG 0.595725 4_2:-9102941 None:intergenic
ATGTGCAGTGTGAGAGCATG+GGG 0.599437 4_2:+9104155 Msa0587530:intron
TAAGTCACTCTGTTTCGAGT+AGG 0.607940 4_2:-9104596 None:intergenic
TCTCAATCATGAAACCATCT+GGG 0.612866 4_2:-9104273 None:intergenic
TAAACGTGTCTATGATGACT+AGG 0.619283 4_2:-9103583 None:intergenic
AATGAATAGAAGATTTGTCA+CGG 0.633905 4_2:-9102615 None:intergenic
TTATGTTCGAGCAAGAGTAG+AGG 0.637893 4_2:+9104213 Msa0587530:three_prime_UTR
AGAACCCAGAGACGTTACCA+AGG 0.641794 4_2:+9103311 Msa0587530:CDS
GTTCGAGCAAGAGTAGAGGA+AGG 0.649580 4_2:+9104217 Msa0587530:three_prime_UTR
ACAATGCATAATATATGCAG+AGG 0.654813 4_2:+9103786 Msa0587530:three_prime_UTR
TAATTCGGAGTTCGACCGTG+AGG 0.657158 4_2:+9104005 Msa0587530:three_prime_UTR
TGTGACCAAACATCTTTGTG+TGG 0.679161 4_2:+9104433 Msa0587530:three_prime_UTR
GTGACCAAACATCTTTGTGT+GGG 0.690684 4_2:+9104434 Msa0587530:three_prime_UTR
TGTGCAGTGTGAGAGCATGG+GGG 0.820385 4_2:+9104156 Msa0587530:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTATATTTAGTTTTAAATT+TGG - chr4_2:9103534-9103553 None:intergenic 10.0%
!! TTAATAAAGTAATGAATTAT+TGG + chr4_2:9104564-9104583 Msa0587530:three_prime_UTR 10.0%
!!! TCTATATTTAGTTTTAAATT+TGG - chr4_2:9103534-9103553 None:intergenic 10.0%
!! TTAATAAAGTAATGAATTAT+TGG + chr4_2:9104564-9104583 Msa0587530:three_prime_UTR 10.0%
!!! ATGTTAAATTTTAGAGAAAA+GGG - chr4_2:9102716-9102735 None:intergenic 15.0%
!!! AATTTAACATCTTTGTTATT+TGG + chr4_2:9102726-9102745 Msa0587530:five_prime_UTR 15.0%
!!! TTTTGTTAAAACAACTATTA+TGG + chr4_2:9103047-9103066 Msa0587530:intron 15.0%
!! TTTGTTAAAACAACTATTAT+GGG + chr4_2:9103048-9103067 Msa0587530:intron 15.0%
!!! GAAAAAATACTAAATTTTGA+GGG - chr4_2:9103175-9103194 None:intergenic 15.0%
!!! AGAAAAAATACTAAATTTTG+AGG - chr4_2:9103176-9103195 None:intergenic 15.0%
!! ACACAAAATTTCATATTAAA+TGG - chr4_2:9104116-9104135 None:intergenic 15.0%
!!! ATGTTAAATTTTAGAGAAAA+GGG - chr4_2:9102716-9102735 None:intergenic 15.0%
!!! AATTTAACATCTTTGTTATT+TGG + chr4_2:9102726-9102745 Msa0587530:five_prime_UTR 15.0%
!!! TTTTGTTAAAACAACTATTA+TGG + chr4_2:9103047-9103066 Msa0587530:intron 15.0%
!! TTTGTTAAAACAACTATTAT+GGG + chr4_2:9103048-9103067 Msa0587530:intron 15.0%
!!! GAAAAAATACTAAATTTTGA+GGG - chr4_2:9103175-9103194 None:intergenic 15.0%
!!! AGAAAAAATACTAAATTTTG+AGG - chr4_2:9103176-9103195 None:intergenic 15.0%
!! ACACAAAATTTCATATTAAA+TGG - chr4_2:9104116-9104135 None:intergenic 15.0%
!!! GATGTTAAATTTTAGAGAAA+AGG - chr4_2:9102717-9102736 None:intergenic 20.0%
!! TTGAACTATGAAAGAAAAAA+CGG - chr4_2:9102756-9102775 None:intergenic 20.0%
!!! TTTTTCTTTCATAGTTCAAT+TGG + chr4_2:9102757-9102776 Msa0587530:five_prime_UTR 20.0%
!!! TTTTCTTTCATAGTTCAATT+GGG + chr4_2:9102758-9102777 Msa0587530:five_prime_UTR 20.0%
!!! TAATTTTCTTGAGAAAATGA+AGG + chr4_2:9102799-9102818 Msa0587530:exon 20.0%
!! AAATTTAATAAACCTTTGCT+TGG + chr4_2:9103200-9103219 Msa0587530:intron 20.0%
!!! TGAAAATTGATTTTTGTATG+TGG + chr4_2:9103232-9103251 Msa0587530:intron 20.0%
!!! ATTTTGAAACGTAATCAATA+AGG - chr4_2:9103402-9103421 None:intergenic 20.0%
!! ATTGATTACGTTTCAAAATT+TGG + chr4_2:9103403-9103422 Msa0587530:CDS 20.0%
!!! GAGTGTTTTAGTTTTAAATT+TGG - chr4_2:9103562-9103581 None:intergenic 20.0%
!! ATCCAACTTTCTTAATTTAA+TGG + chr4_2:9103893-9103912 Msa0587530:three_prime_UTR 20.0%
!! TACCATTAAATTAAGAAAGT+TGG - chr4_2:9103898-9103917 None:intergenic 20.0%
!!! GATGTTAAATTTTAGAGAAA+AGG - chr4_2:9102717-9102736 None:intergenic 20.0%
!! TTGAACTATGAAAGAAAAAA+CGG - chr4_2:9102756-9102775 None:intergenic 20.0%
!!! TTTTTCTTTCATAGTTCAAT+TGG + chr4_2:9102757-9102776 Msa0587530:five_prime_UTR 20.0%
!!! TTTTCTTTCATAGTTCAATT+GGG + chr4_2:9102758-9102777 Msa0587530:five_prime_UTR 20.0%
!!! TAATTTTCTTGAGAAAATGA+AGG + chr4_2:9102799-9102818 Msa0587530:exon 20.0%
!! AAATTTAATAAACCTTTGCT+TGG + chr4_2:9103200-9103219 Msa0587530:intron 20.0%
!!! TGAAAATTGATTTTTGTATG+TGG + chr4_2:9103232-9103251 Msa0587530:intron 20.0%
!!! ATTTTGAAACGTAATCAATA+AGG - chr4_2:9103402-9103421 None:intergenic 20.0%
!! ATTGATTACGTTTCAAAATT+TGG + chr4_2:9103403-9103422 Msa0587530:CDS 20.0%
!!! GAGTGTTTTAGTTTTAAATT+TGG - chr4_2:9103562-9103581 None:intergenic 20.0%
!! ATCCAACTTTCTTAATTTAA+TGG + chr4_2:9103893-9103912 Msa0587530:three_prime_UTR 20.0%
!! TACCATTAAATTAAGAAAGT+TGG - chr4_2:9103898-9103917 None:intergenic 20.0%
! AATGAATAGAAGATTTGTCA+CGG - chr4_2:9102618-9102637 None:intergenic 25.0%
! GTGATCACAAATTATTGTTA+AGG - chr4_2:9102669-9102688 None:intergenic 25.0%
!!! AGATATGATTCACTGATTTT+AGG - chr4_2:9102847-9102866 None:intergenic 25.0%
! GAAGAAAGAAATAAATCCAA+AGG + chr4_2:9103333-9103352 Msa0587530:CDS 25.0%
! AAAGATATGTGATTATCCTT+TGG - chr4_2:9103352-9103371 None:intergenic 25.0%
!! CGTTTATGTTATCCTTTTTA+AGG + chr4_2:9103600-9103619 Msa0587530:three_prime_UTR 25.0%
! GTGCAAAAAATTACATGATT+CGG - chr4_2:9103692-9103711 None:intergenic 25.0%
! GTTTCATCAGAAATTAAACT+TGG + chr4_2:9104051-9104070 Msa0587530:intron 25.0%
!! CTCAACAATTTGTCATTTAT+CGG + chr4_2:9104080-9104099 Msa0587530:intron 25.0%
! TTTGTGTTTGAACTTTGAAT+TGG + chr4_2:9104129-9104148 Msa0587530:intron 25.0%
! AATGAATAGAAGATTTGTCA+CGG - chr4_2:9102618-9102637 None:intergenic 25.0%
! GTGATCACAAATTATTGTTA+AGG - chr4_2:9102669-9102688 None:intergenic 25.0%
!!! AGATATGATTCACTGATTTT+AGG - chr4_2:9102847-9102866 None:intergenic 25.0%
! GAAGAAAGAAATAAATCCAA+AGG + chr4_2:9103333-9103352 Msa0587530:CDS 25.0%
! AAAGATATGTGATTATCCTT+TGG - chr4_2:9103352-9103371 None:intergenic 25.0%
!! CGTTTATGTTATCCTTTTTA+AGG + chr4_2:9103600-9103619 Msa0587530:three_prime_UTR 25.0%
! GTGCAAAAAATTACATGATT+CGG - chr4_2:9103692-9103711 None:intergenic 25.0%
! GTTTCATCAGAAATTAAACT+TGG + chr4_2:9104051-9104070 Msa0587530:intron 25.0%
!! CTCAACAATTTGTCATTTAT+CGG + chr4_2:9104080-9104099 Msa0587530:intron 25.0%
! TTTGTGTTTGAACTTTGAAT+TGG + chr4_2:9104129-9104148 Msa0587530:intron 25.0%
TCATTTAGAAAACCAAGCAA+AGG - chr4_2:9103215-9103234 None:intergenic 30.0%
! GGATTTATTTCTTTCTTCCT+TGG - chr4_2:9103331-9103350 None:intergenic 30.0%
AGACGTACTTCAATAAATGA+AGG + chr4_2:9103461-9103480 Msa0587530:three_prime_UTR 30.0%
!!! TTTTTCTATGCGTCACTTTT+AGG - chr4_2:9103510-9103529 None:intergenic 30.0%
!! TCCTTTTTAAGGATATGCAA+TGG + chr4_2:9103611-9103630 Msa0587530:three_prime_UTR 30.0%
!!! GATATGCAATGGCTTTTTTA+AGG + chr4_2:9103622-9103641 Msa0587530:three_prime_UTR 30.0%
TTTAAGGATCTTATCCTCTA+AGG + chr4_2:9103638-9103657 Msa0587530:three_prime_UTR 30.0%
!!! ATGTAATTTTTTGCACTGCA+CGG + chr4_2:9103696-9103715 Msa0587530:three_prime_UTR 30.0%
ACAATGCATAATATATGCAG+AGG + chr4_2:9103786-9103805 Msa0587530:three_prime_UTR 30.0%
!!! TTTTTTATCAAGGAGACTAG+TGG - chr4_2:9103861-9103880 None:intergenic 30.0%
CGATAAATGACAAATTGTTG+AGG - chr4_2:9104082-9104101 None:intergenic 30.0%
CTTATTTGGACTGCTATTAT+TGG + chr4_2:9104615-9104634 Msa0587530:three_prime_UTR 30.0%
TCATTTAGAAAACCAAGCAA+AGG - chr4_2:9103215-9103234 None:intergenic 30.0%
! GGATTTATTTCTTTCTTCCT+TGG - chr4_2:9103331-9103350 None:intergenic 30.0%
AGACGTACTTCAATAAATGA+AGG + chr4_2:9103461-9103480 Msa0587530:three_prime_UTR 30.0%
!!! TTTTTCTATGCGTCACTTTT+AGG - chr4_2:9103510-9103529 None:intergenic 30.0%
!! TCCTTTTTAAGGATATGCAA+TGG + chr4_2:9103611-9103630 Msa0587530:three_prime_UTR 30.0%
!!! GATATGCAATGGCTTTTTTA+AGG + chr4_2:9103622-9103641 Msa0587530:three_prime_UTR 30.0%
TTTAAGGATCTTATCCTCTA+AGG + chr4_2:9103638-9103657 Msa0587530:three_prime_UTR 30.0%
!!! ATGTAATTTTTTGCACTGCA+CGG + chr4_2:9103696-9103715 Msa0587530:three_prime_UTR 30.0%
ACAATGCATAATATATGCAG+AGG + chr4_2:9103786-9103805 Msa0587530:three_prime_UTR 30.0%
!!! TTTTTTATCAAGGAGACTAG+TGG - chr4_2:9103861-9103880 None:intergenic 30.0%
CGATAAATGACAAATTGTTG+AGG - chr4_2:9104082-9104101 None:intergenic 30.0%
CTTATTTGGACTGCTATTAT+TGG + chr4_2:9104615-9104634 Msa0587530:three_prime_UTR 30.0%
TGTTAAGGAAGAGAAGAAAG+AGG - chr4_2:9102654-9102673 None:intergenic 35.0%
CTTTCATAGTTCAATTGGGT+TGG + chr4_2:9102762-9102781 Msa0587530:five_prime_UTR 35.0%
TGAGAAAATGAAGGAAAGTG+TGG + chr4_2:9102808-9102827 Msa0587530:exon 35.0%
! GAGAAAATGAAGGAAAGTGT+GGG + chr4_2:9102809-9102828 Msa0587530:exon 35.0%
!!! TCACTGATTTTAGGTGCAAA+GGG - chr4_2:9102838-9102857 None:intergenic 35.0%
!!! TTCACTGATTTTAGGTGCAA+AGG - chr4_2:9102839-9102858 None:intergenic 35.0%
GATTTCTACCAGAATGTTCT+TGG + chr4_2:9102899-9102918 Msa0587530:CDS 35.0%
TTGGTTTCTTCCCAATAAGA+AGG + chr4_2:9102918-9102937 Msa0587530:CDS 35.0%
CCATCAAAATCAAAAGAACC+AGG - chr4_2:9102944-9102963 None:intergenic 35.0%
!!! CCTGGTTCTTTTGATTTTGA+TGG + chr4_2:9102941-9102960 Msa0587530:CDS 35.0%
!!! CTTTTGATTTTGATGGAGCA+TGG + chr4_2:9102948-9102967 Msa0587530:CDS 35.0%
GGATAATCACATATCTTTCC+AGG + chr4_2:9103354-9103373 Msa0587530:CDS 35.0%
GTTTAGTGAAAAATCTGACC+TGG - chr4_2:9103375-9103394 None:intergenic 35.0%
TAAACGTGTCTATGATGACT+AGG - chr4_2:9103586-9103605 None:intergenic 35.0%
GCCATTGCATATCCTTAAAA+AGG - chr4_2:9103615-9103634 None:intergenic 35.0%
TAGTCTATGTGAGCTTAACT+CGG + chr4_2:9103754-9103773 Msa0587530:three_prime_UTR 35.0%
TGCATAATATATGCAGAGGT+CGG + chr4_2:9103790-9103809 Msa0587530:three_prime_UTR 35.0%
TTGTTAACCATTCGATTCTC+AGG + chr4_2:9103968-9103987 Msa0587530:three_prime_UTR 35.0%
TGTTAACCATTCGATTCTCA+GGG + chr4_2:9103969-9103988 Msa0587530:three_prime_UTR 35.0%
ATCAGACTATACTTACCTCA+CGG - chr4_2:9104023-9104042 None:intergenic 35.0%
GGAGAAAAAGCTGAAAGAAA+TGG + chr4_2:9104183-9104202 Msa0587530:three_prime_UTR 35.0%
TCTCAATCATGAAACCATCT+GGG - chr4_2:9104276-9104295 None:intergenic 35.0%
ATCTCAATCATGAAACCATC+TGG - chr4_2:9104277-9104296 None:intergenic 35.0%
! TTTTCCCACACAAAGATGTT+TGG - chr4_2:9104441-9104460 None:intergenic 35.0%
AAACATCTTTGTGTGGGAAA+AGG + chr4_2:9104440-9104459 Msa0587530:three_prime_UTR 35.0%
TCGAAACAGAGTGACTTATT+TGG + chr4_2:9104601-9104620 Msa0587530:three_prime_UTR 35.0%
TGTTAAGGAAGAGAAGAAAG+AGG - chr4_2:9102654-9102673 None:intergenic 35.0%
CTTTCATAGTTCAATTGGGT+TGG + chr4_2:9102762-9102781 Msa0587530:five_prime_UTR 35.0%
TGAGAAAATGAAGGAAAGTG+TGG + chr4_2:9102808-9102827 Msa0587530:exon 35.0%
! GAGAAAATGAAGGAAAGTGT+GGG + chr4_2:9102809-9102828 Msa0587530:exon 35.0%
!!! TCACTGATTTTAGGTGCAAA+GGG - chr4_2:9102838-9102857 None:intergenic 35.0%
!!! TTCACTGATTTTAGGTGCAA+AGG - chr4_2:9102839-9102858 None:intergenic 35.0%
GATTTCTACCAGAATGTTCT+TGG + chr4_2:9102899-9102918 Msa0587530:CDS 35.0%
TTGGTTTCTTCCCAATAAGA+AGG + chr4_2:9102918-9102937 Msa0587530:CDS 35.0%
CCATCAAAATCAAAAGAACC+AGG - chr4_2:9102944-9102963 None:intergenic 35.0%
!!! CCTGGTTCTTTTGATTTTGA+TGG + chr4_2:9102941-9102960 Msa0587530:CDS 35.0%
!!! CTTTTGATTTTGATGGAGCA+TGG + chr4_2:9102948-9102967 Msa0587530:CDS 35.0%
GGATAATCACATATCTTTCC+AGG + chr4_2:9103354-9103373 Msa0587530:CDS 35.0%
GTTTAGTGAAAAATCTGACC+TGG - chr4_2:9103375-9103394 None:intergenic 35.0%
TAAACGTGTCTATGATGACT+AGG - chr4_2:9103586-9103605 None:intergenic 35.0%
GCCATTGCATATCCTTAAAA+AGG - chr4_2:9103615-9103634 None:intergenic 35.0%
TAGTCTATGTGAGCTTAACT+CGG + chr4_2:9103754-9103773 Msa0587530:three_prime_UTR 35.0%
TGCATAATATATGCAGAGGT+CGG + chr4_2:9103790-9103809 Msa0587530:three_prime_UTR 35.0%
TTGTTAACCATTCGATTCTC+AGG + chr4_2:9103968-9103987 Msa0587530:three_prime_UTR 35.0%
TGTTAACCATTCGATTCTCA+GGG + chr4_2:9103969-9103988 Msa0587530:three_prime_UTR 35.0%
ATCAGACTATACTTACCTCA+CGG - chr4_2:9104023-9104042 None:intergenic 35.0%
GGAGAAAAAGCTGAAAGAAA+TGG + chr4_2:9104183-9104202 Msa0587530:three_prime_UTR 35.0%
TCTCAATCATGAAACCATCT+GGG - chr4_2:9104276-9104295 None:intergenic 35.0%
ATCTCAATCATGAAACCATC+TGG - chr4_2:9104277-9104296 None:intergenic 35.0%
! TTTTCCCACACAAAGATGTT+TGG - chr4_2:9104441-9104460 None:intergenic 35.0%
AAACATCTTTGTGTGGGAAA+AGG + chr4_2:9104440-9104459 Msa0587530:three_prime_UTR 35.0%
TCGAAACAGAGTGACTTATT+TGG + chr4_2:9104601-9104620 Msa0587530:three_prime_UTR 35.0%
GGAAGAAACCAAGAACATTC+TGG - chr4_2:9102910-9102929 None:intergenic 40.0%
AAGAACCAGGCCTTCTTATT+GGG - chr4_2:9102931-9102950 None:intergenic 40.0%
AAAGAACCAGGCCTTCTTAT+TGG - chr4_2:9102932-9102951 None:intergenic 40.0%
! TATGTGGTGACCTAATTTGC+AGG + chr4_2:9103248-9103267 Msa0587530:intron 40.0%
! GACCTAATTTGCAGGCTATT+TGG + chr4_2:9103256-9103275 Msa0587530:intron 40.0%
AGCCAAATAGCCTGCAAATT+AGG - chr4_2:9103261-9103280 None:intergenic 40.0%
ATTTGCAGGCTATTTGGCTA+TGG + chr4_2:9103262-9103281 Msa0587530:intron 40.0%
AGGCTATTTGGCTATGGTAT+TGG + chr4_2:9103268-9103287 Msa0587530:intron 40.0%
! GCAATTTGAAGCAACCTTAG+AGG - chr4_2:9103655-9103674 None:intergenic 40.0%
GCATAATATATGCAGAGGTC+GGG + chr4_2:9103791-9103810 Msa0587530:three_prime_UTR 40.0%
CATAATATATGCAGAGGTCG+GGG + chr4_2:9103792-9103811 Msa0587530:three_prime_UTR 40.0%
!! CATTTTAAACACGAGGTGTC+TGG - chr4_2:9103826-9103845 None:intergenic 40.0%
! GCTCACACATTTTAAACACG+AGG - chr4_2:9103833-9103852 None:intergenic 40.0%
GCATCGTGCAATTGTAGTAA+AGG + chr4_2:9103920-9103939 Msa0587530:three_prime_UTR 40.0%
! TCTTTTCCCTGAGAATCGAA+TGG - chr4_2:9103978-9103997 None:intergenic 40.0%
! GGAAAAGAGCTCTGGTAATT+CGG + chr4_2:9103990-9104009 Msa0587530:three_prime_UTR 40.0%
TTATGTTCGAGCAAGAGTAG+AGG + chr4_2:9104213-9104232 Msa0587530:three_prime_UTR 40.0%
!! CTATTTTTCCATGACCCAGA+TGG + chr4_2:9104259-9104278 Msa0587530:three_prime_UTR 40.0%
CTTCCTGTGATTCCATTAGT+TGG + chr4_2:9104313-9104332 Msa0587530:three_prime_UTR 40.0%
TCACCAACTAATGGAATCAC+AGG - chr4_2:9104319-9104338 None:intergenic 40.0%
! TGTGATTCCATTAGTTGGTG+AGG + chr4_2:9104318-9104337 Msa0587530:three_prime_UTR 40.0%
TGTGACCAAACATCTTTGTG+TGG + chr4_2:9104433-9104452 Msa0587530:three_prime_UTR 40.0%
GTGACCAAACATCTTTGTGT+GGG + chr4_2:9104434-9104453 Msa0587530:three_prime_UTR 40.0%
! TAAGTCACTCTGTTTCGAGT+AGG - chr4_2:9104599-9104618 None:intergenic 40.0%
GGAAGAAACCAAGAACATTC+TGG - chr4_2:9102910-9102929 None:intergenic 40.0%
AAGAACCAGGCCTTCTTATT+GGG - chr4_2:9102931-9102950 None:intergenic 40.0%
AAAGAACCAGGCCTTCTTAT+TGG - chr4_2:9102932-9102951 None:intergenic 40.0%
! TATGTGGTGACCTAATTTGC+AGG + chr4_2:9103248-9103267 Msa0587530:intron 40.0%
! GACCTAATTTGCAGGCTATT+TGG + chr4_2:9103256-9103275 Msa0587530:intron 40.0%
AGCCAAATAGCCTGCAAATT+AGG - chr4_2:9103261-9103280 None:intergenic 40.0%
ATTTGCAGGCTATTTGGCTA+TGG + chr4_2:9103262-9103281 Msa0587530:intron 40.0%
AGGCTATTTGGCTATGGTAT+TGG + chr4_2:9103268-9103287 Msa0587530:intron 40.0%
! GCAATTTGAAGCAACCTTAG+AGG - chr4_2:9103655-9103674 None:intergenic 40.0%
GCATAATATATGCAGAGGTC+GGG + chr4_2:9103791-9103810 Msa0587530:three_prime_UTR 40.0%
CATAATATATGCAGAGGTCG+GGG + chr4_2:9103792-9103811 Msa0587530:three_prime_UTR 40.0%
!! CATTTTAAACACGAGGTGTC+TGG - chr4_2:9103826-9103845 None:intergenic 40.0%
! GCTCACACATTTTAAACACG+AGG - chr4_2:9103833-9103852 None:intergenic 40.0%
GCATCGTGCAATTGTAGTAA+AGG + chr4_2:9103920-9103939 Msa0587530:three_prime_UTR 40.0%
! TCTTTTCCCTGAGAATCGAA+TGG - chr4_2:9103978-9103997 None:intergenic 40.0%
! GGAAAAGAGCTCTGGTAATT+CGG + chr4_2:9103990-9104009 Msa0587530:three_prime_UTR 40.0%
TTATGTTCGAGCAAGAGTAG+AGG + chr4_2:9104213-9104232 Msa0587530:three_prime_UTR 40.0%
!! CTATTTTTCCATGACCCAGA+TGG + chr4_2:9104259-9104278 Msa0587530:three_prime_UTR 40.0%
CTTCCTGTGATTCCATTAGT+TGG + chr4_2:9104313-9104332 Msa0587530:three_prime_UTR 40.0%
TCACCAACTAATGGAATCAC+AGG - chr4_2:9104319-9104338 None:intergenic 40.0%
! TGTGATTCCATTAGTTGGTG+AGG + chr4_2:9104318-9104337 Msa0587530:three_prime_UTR 40.0%
TGTGACCAAACATCTTTGTG+TGG + chr4_2:9104433-9104452 Msa0587530:three_prime_UTR 40.0%
GTGACCAAACATCTTTGTGT+GGG + chr4_2:9104434-9104453 Msa0587530:three_prime_UTR 40.0%
! TAAGTCACTCTGTTTCGAGT+AGG - chr4_2:9104599-9104618 None:intergenic 40.0%
TTCTTCCCAATAAGAAGGCC+TGG + chr4_2:9102923-9102942 Msa0587530:CDS 45.0%
!! TCTTCCTTGGTAACGTCTCT+GGG - chr4_2:9103318-9103337 None:intergenic 45.0%
!! TTCTTCCTTGGTAACGTCTC+TGG - chr4_2:9103319-9103338 None:intergenic 45.0%
CTATGTGAGCTTAACTCGGT+TGG + chr4_2:9103758-9103777 Msa0587530:three_prime_UTR 45.0%
! TGAGCTTAACTCGGTTGGTA+GGG + chr4_2:9103763-9103782 Msa0587530:three_prime_UTR 45.0%
ATTCTCAGGGAAAAGAGCTC+TGG + chr4_2:9103982-9104001 Msa0587530:three_prime_UTR 45.0%
TCATGAAACCATCTGGGTCA+TGG - chr4_2:9104270-9104289 None:intergenic 45.0%
CTTGCTACCTCACCAACTAA+TGG - chr4_2:9104328-9104347 None:intergenic 45.0%
TTCTTCCCAATAAGAAGGCC+TGG + chr4_2:9102923-9102942 Msa0587530:CDS 45.0%
!! TCTTCCTTGGTAACGTCTCT+GGG - chr4_2:9103318-9103337 None:intergenic 45.0%
!! TTCTTCCTTGGTAACGTCTC+TGG - chr4_2:9103319-9103338 None:intergenic 45.0%
CTATGTGAGCTTAACTCGGT+TGG + chr4_2:9103758-9103777 Msa0587530:three_prime_UTR 45.0%
! TGAGCTTAACTCGGTTGGTA+GGG + chr4_2:9103763-9103782 Msa0587530:three_prime_UTR 45.0%
ATTCTCAGGGAAAAGAGCTC+TGG + chr4_2:9103982-9104001 Msa0587530:three_prime_UTR 45.0%
TCATGAAACCATCTGGGTCA+TGG - chr4_2:9104270-9104289 None:intergenic 45.0%
CTTGCTACCTCACCAACTAA+TGG - chr4_2:9104328-9104347 None:intergenic 45.0%
!!! TAATATTTTTTTTTTTATCA+AGG - chr4_2:9103871-9103890 None:intergenic 5.0%
!! TTATTAATAATTAACATATA+AGG - chr4_2:9104551-9104570 None:intergenic 5.0%
!!! TAATATTTTTTTTTTTATCA+AGG - chr4_2:9103871-9103890 None:intergenic 5.0%
!! TTATTAATAATTAACATATA+AGG - chr4_2:9104551-9104570 None:intergenic 5.0%
AGAACCCAGAGACGTTACCA+AGG + chr4_2:9103311-9103330 Msa0587530:CDS 50.0%
GTGAGCTTAACTCGGTTGGT+AGG + chr4_2:9103762-9103781 Msa0587530:three_prime_UTR 50.0%
TAATTCGGAGTTCGACCGTG+AGG + chr4_2:9104005-9104024 Msa0587530:three_prime_UTR 50.0%
TGATGTGCAGTGTGAGAGCA+TGG + chr4_2:9104153-9104172 Msa0587530:intron 50.0%
! GATGTGCAGTGTGAGAGCAT+GGG + chr4_2:9104154-9104173 Msa0587530:intron 50.0%
! ATGTGCAGTGTGAGAGCATG+GGG + chr4_2:9104155-9104174 Msa0587530:intron 50.0%
GTTCGAGCAAGAGTAGAGGA+AGG + chr4_2:9104217-9104236 Msa0587530:three_prime_UTR 50.0%
GAGCAAGAGTAGAGGAAGGT+GGG + chr4_2:9104221-9104240 Msa0587530:three_prime_UTR 50.0%
AGAGGAAGGTGGGATTGAAG+TGG + chr4_2:9104231-9104250 Msa0587530:three_prime_UTR 50.0%
AGAACCCAGAGACGTTACCA+AGG + chr4_2:9103311-9103330 Msa0587530:CDS 50.0%
GTGAGCTTAACTCGGTTGGT+AGG + chr4_2:9103762-9103781 Msa0587530:three_prime_UTR 50.0%
TAATTCGGAGTTCGACCGTG+AGG + chr4_2:9104005-9104024 Msa0587530:three_prime_UTR 50.0%
TGATGTGCAGTGTGAGAGCA+TGG + chr4_2:9104153-9104172 Msa0587530:intron 50.0%
! GATGTGCAGTGTGAGAGCAT+GGG + chr4_2:9104154-9104173 Msa0587530:intron 50.0%
! ATGTGCAGTGTGAGAGCATG+GGG + chr4_2:9104155-9104174 Msa0587530:intron 50.0%
GTTCGAGCAAGAGTAGAGGA+AGG + chr4_2:9104217-9104236 Msa0587530:three_prime_UTR 50.0%
GAGCAAGAGTAGAGGAAGGT+GGG + chr4_2:9104221-9104240 Msa0587530:three_prime_UTR 50.0%
AGAGGAAGGTGGGATTGAAG+TGG + chr4_2:9104231-9104250 Msa0587530:three_prime_UTR 50.0%
! TGTGCAGTGTGAGAGCATGG+GGG + chr4_2:9104156-9104175 Msa0587530:intron 55.0%
CGAGCAAGAGTAGAGGAAGG+TGG + chr4_2:9104220-9104239 Msa0587530:three_prime_UTR 55.0%
! TGTGCAGTGTGAGAGCATGG+GGG + chr4_2:9104156-9104175 Msa0587530:intron 55.0%
CGAGCAAGAGTAGAGGAAGG+TGG + chr4_2:9104220-9104239 Msa0587530:three_prime_UTR 55.0%
! GTGTGAGAGCATGGGGGCAG+TGG + chr4_2:9104162-9104181 Msa0587530:intron 65.0%
! GTGTGAGAGCATGGGGGCAG+TGG + chr4_2:9104162-9104181 Msa0587530:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 9102615 9104639 9102615 ID=Msa0587530;Name=Msa0587530
chr4_2 mRNA 9102615 9104639 9102615 ID=Msa0587530-mRNA-1;Parent=Msa0587530;Name=Msa0587530-mRNA-1;_AED=0.04;_eAED=0.04;_QI=200|1|1|1|0.5|0.66|3|1059|109
chr4_2 exon 9102615 9102969 9102615 ID=Msa0587530-mRNA-1:exon:2507;Parent=Msa0587530-mRNA-1
chr4_2 exon 9103270 9104026 9103270 ID=Msa0587530-mRNA-1:exon:2508;Parent=Msa0587530-mRNA-1
chr4_2 exon 9104163 9104639 9104163 ID=Msa0587530-mRNA-1:exon:2509;Parent=Msa0587530-mRNA-1
chr4_2 five_prime_UTR 9102615 9102814 9102615 ID=Msa0587530-mRNA-1:five_prime_utr;Parent=Msa0587530-mRNA-1
chr4_2 CDS 9102815 9102969 9102815 ID=Msa0587530-mRNA-1:cds;Parent=Msa0587530-mRNA-1
chr4_2 CDS 9103270 9103444 9103270 ID=Msa0587530-mRNA-1:cds;Parent=Msa0587530-mRNA-1
chr4_2 three_prime_UTR 9103445 9104026 9103445 ID=Msa0587530-mRNA-1:three_prime_utr;Parent=Msa0587530-mRNA-1
chr4_2 three_prime_UTR 9104163 9104639 9104163 ID=Msa0587530-mRNA-1:three_prime_utr;Parent=Msa0587530-mRNA-1
Gene Sequence

>Msa0587530

ATGAAGGAAAGTGTGGGAAACCCTTTGCACCTAAAATCAGTGAATCATATCTCACTCATATGCAGATCAGTTGAAGAGTCAATAGATTTCTACCAGAATGTTCTTGGTTTCTTCCCAATAAGAAGGCCTGGTTCTTTTGATTTTGATGGAGCATGGCTATTTGGCTATGGTATTGGTATTCATTTGCTAGAAGCAGAGAACCCAGAGACGTTACCAAGGAAGAAAGAAATAAATCCAAAGGATAATCACATATCTTTCCAGGTCAGATTTTTCACTAAACTTAACCTTATTGATTACGTTTCAAAATTTGGAAAAAATAATGTGACTTAG
Protein sequence

>Msa0587530

MKESVGNPLHLKSVNHISLICRSVEESIDFYQNVLGFFPIRRPGSFDFDGAWLFGYGIGIHLLEAENPETLPRKKEINPKDNHISFQVRFFTKLNLIDYVSKFGKNNVT*