AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0627770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0627770 MtrunA17_Chr4g0003581 97.753 89 2 0 1 89 1 89 2.84e-61 184
Msa0627770 MtrunA17_Chr2g0332851 79.545 88 18 0 1 88 1 88 2.12e-48 152
Msa0627770 MtrunA17_Chr2g0332841 67.961 103 28 1 1 103 1 98 1.20e-44 142
Msa0627770 MtrunA17_Chr4g0063501 67.059 85 28 0 5 89 1 85 1.95e-41 134
Msa0627770 MtrunA17_Chr2g0277151 61.728 81 31 0 8 88 2 82 3.53e-32 111
Msa0627770 MtrunA17_Chr5g0405171 55.056 89 32 2 6 88 22 108 1.11e-28 102
Msa0627770 MtrunA17_Chr4g0020201 52.747 91 38 2 2 89 29 117 5.43e-28 100
Msa0627770 MtrunA17_Chr8g0372141 55.294 85 34 1 8 88 12 96 2.08e-27 99.4
Msa0627770 MtrunA17_Chr1g0212241 67.692 65 21 0 24 88 1 65 4.51e-27 97.1
Msa0627770 MtrunA17_Chr4g0070901 35.632 87 54 1 13 97 72 158 1.50e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0627770 AT1G80160.1 77.528 89 20 0 1 89 1 89 1.57e-48 152
Msa0627770 AT1G80160.3 78.409 88 19 0 1 88 42 129 3.64e-48 153
Msa0627770 AT1G15380.2 70.787 89 26 0 1 89 1 89 2.21e-44 142
Msa0627770 AT1G15380.1 70.787 89 26 0 1 89 1 89 2.21e-44 142
Msa0627770 AT2G28420.1 51.765 85 40 1 11 94 18 102 2.11e-28 102
Msa0627770 AT1G80160.2 72.222 36 10 0 53 88 27 62 7.03e-12 58.5
Msa0627770 AT5G57040.1 34.409 93 57 2 14 104 79 169 2.34e-11 58.2

Find 69 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTAACATCTTTGTTATT+TGG 0.109561 4_3:-8203035 Msa0627770:five_prime_UTR
ATTGATTACGTTTCAAAATT+TGG 0.126932 4_3:-8202298 Msa0627770:CDS
ATCCAACTTTCTTAATTTAA+TGG 0.127326 4_3:-8201795 Msa0627770:three_prime_UTR
TTAATAAAGTAATGAATTAT+TGG 0.150400 4_3:-8201120 Msa0627770:three_prime_UTR
AAAGAACCAGGCCTTCTTAT+TGG 0.200598 4_3:+8202832 None:intergenic
TTCTTCCTTGGTAACGTCTC+TGG 0.223323 4_3:+8202385 None:intergenic
TTAATAATATTTAACATATA+AGG 0.239142 4_3:+8201139 None:intergenic
GCCATTGCATATCCTTAAAA+AGG 0.260168 4_3:+8202078 None:intergenic
GACCAAATTTGCAGGCTATT+TGG 0.275406 4_3:-8202445 Msa0627770:intron
GATTTCTACCAGAATGTTCT+TGG 0.277645 4_3:-8202862 Msa0627770:CDS
TCGAAACAGAGTGACTTATT+TGG 0.284927 4_3:-8201083 Msa0627770:three_prime_UTR
TTTAAGGATCTTATCCTCTA+AGG 0.285109 4_3:-8202054 Msa0627770:three_prime_UTR
GGATTTATTTCTTTCTTCCT+TGG 0.309354 4_3:+8202373 None:intergenic
CTTATTTGGACTGATATTAT+TGG 0.312151 4_3:-8201069 Msa0627770:three_prime_UTR
AAGAACCAGGCCTTCTTATT+GGG 0.313845 4_3:+8202833 None:intergenic
GCAATTTGAAGCAACCTTAG+AGG 0.323527 4_3:+8202040 None:intergenic
TTGTTAACCATTCGATTCTC+AGG 0.349832 4_3:-8201719 Msa0627770:three_prime_UTR
AGGCTATTTGGCTATGGTAT+TGG 0.365518 4_3:-8202433 Msa0627770:CDS
TACCATTAAATTAAGAAAGT+TGG 0.369024 4_3:+8201793 None:intergenic
ATCTCAATCATGAAACCATC+TGG 0.372159 4_3:+8201413 None:intergenic
CTTGCTACCTCACCAACTAA+TGG 0.386113 4_3:+8201362 None:intergenic
GTGATCACAAATTATTGTTA+AGG 0.387388 4_3:+8203095 None:intergenic
AAAGATATGTGATTATCCTT+TGG 0.393062 4_3:+8202352 None:intergenic
GGAAGAAACCAAGAACATTC+TGG 0.404879 4_3:+8202854 None:intergenic
GGATAATCACATATCTTTCC+AGG 0.407468 4_3:-8202347 Msa0627770:CDS
CTTTCATAGTTCAATTGGGT+TGG 0.410235 4_3:-8202999 Msa0627770:five_prime_UTR
GGAAAAGAGCTCTGGTAATT+CGG 0.412673 4_3:-8201697 Msa0627770:three_prime_UTR
CTTCCTGTGATTCCATTAGT+TGG 0.419159 4_3:-8201374 Msa0627770:three_prime_UTR
TCTTCCTTGGTAACGTCTCT+GGG 0.440829 4_3:+8202386 None:intergenic
AAACATCTTTGTGTGGGAAA+AGG 0.452378 4_3:-8201247 Msa0627770:three_prime_UTR
TTGGTTTCTTCCCAATAAGA+AGG 0.469291 4_3:-8202843 Msa0627770:CDS
TTCTTCCCAATAAGAAGGCC+TGG 0.475428 4_3:-8202838 Msa0627770:CDS
TGTTAAGGAAGAGAAGAAAG+AGG 0.482716 4_3:+8203110 None:intergenic
ATTTGCAGGCTATTTGGCTA+TGG 0.495339 4_3:-8202439 Msa0627770:intron
GCATCGTGCAATTGTAGTAA+AGG 0.495935 4_3:-8201768 Msa0627770:three_prime_UTR
TGATGTGCAGTGTGAGAGCA+TGG 0.503338 4_3:-8201534 Msa0627770:intron
TCATGAAACCATCTGGGTCA+TGG 0.503506 4_3:+8201420 None:intergenic
TGCATAATATATGCAGAGGT+AGG 0.508314 4_3:-8201897 Msa0627770:three_prime_UTR
GATGTGCAGTGTGAGAGCAT+GGG 0.508852 4_3:-8201533 Msa0627770:intron
ATTCTCAGGGAAAAGAGCTC+TGG 0.513593 4_3:-8201705 Msa0627770:three_prime_UTR
GTGAGCTTAACTCGGTTGGT+AGG 0.517952 4_3:-8201925 Msa0627770:three_prime_UTR
GCATAATATATGCAGAGGTA+GGG 0.518907 4_3:-8201896 Msa0627770:three_prime_UTR
TGAGAAAATGAAGGAAAGTG+TGG 0.520447 4_3:-8202953 Msa0627770:exon
AGAGGAAGGTGGGATTGAAG+TGG 0.523433 4_3:-8201456 Msa0627770:three_prime_UTR
TGAGCTTAACTCGGTTGGTA+GGG 0.544300 4_3:-8201924 Msa0627770:three_prime_UTR
TAAGTCACTCTGTTTCGAGC+AGG 0.545751 4_3:+8201088 None:intergenic
TCACCAACTAATGGAATCAC+AGG 0.547180 4_3:+8201371 None:intergenic
TGTGATTCCATTAGTTGGTG+AGG 0.549148 4_3:-8201369 Msa0627770:three_prime_UTR
CATAATATATGCAGAGGTAG+GGG 0.560087 4_3:-8201895 Msa0627770:three_prime_UTR
CTACGTGAGCTTAACTCGGT+TGG 0.566531 4_3:-8201929 Msa0627770:three_prime_UTR
TGTTAACCATTCGATTCTCA+GGG 0.568057 4_3:-8201718 Msa0627770:three_prime_UTR
GAGCAAGAGTAGAGGAAGGT+GGG 0.575892 4_3:-8201466 Msa0627770:three_prime_UTR
GAGAAAATGAAGGAAAGTGT+GGG 0.578884 4_3:-8202952 Msa0627770:exon
CGAGCAAGAGTAGAGGAAGG+TGG 0.581051 4_3:-8201467 Msa0627770:three_prime_UTR
GAAGAAAGAAATAAATCCAA+AGG 0.593095 4_3:-8202368 Msa0627770:CDS
CCATCAAAATCAAAAGAACC+AGG 0.594472 4_3:+8202820 None:intergenic
ATGTGCAGTGTGAGAGCATG+GGG 0.599437 4_3:-8201532 Msa0627770:intron
AATGAATAGAAGATTTGTCA+CGG 0.602450 4_3:+8203146 None:intergenic
AAGTCTACGTGAGCTTAACT+CGG 0.612263 4_3:-8201933 Msa0627770:three_prime_UTR
TCTCAATCATGAAACCATCT+GGG 0.612866 4_3:+8201414 None:intergenic
TAAACGTGTCTATGATGACT+AGG 0.619283 4_3:+8202107 None:intergenic
TTATGTTCGAGCAAGAGTAG+AGG 0.637893 4_3:-8201474 Msa0627770:three_prime_UTR
AGAACCCAGAGACGTTACCA+AGG 0.641794 4_3:-8202390 Msa0627770:CDS
GTTCGAGCAAGAGTAGAGGA+AGG 0.649580 4_3:-8201470 Msa0627770:three_prime_UTR
ACAATGCATAATATATGCAG+AGG 0.654813 4_3:-8201901 Msa0627770:three_prime_UTR
TAATTCGGAGTTCGACCGTG+AGG 0.657158 4_3:-8201682 Msa0627770:intron
TGTGACCAAACATCTTTGTG+TGG 0.679161 4_3:-8201254 Msa0627770:three_prime_UTR
GTGACCAAACATCTTTGTGT+GGG 0.690684 4_3:-8201253 Msa0627770:three_prime_UTR
TGTGCAGTGTGAGAGCATGG+GGG 0.820385 4_3:-8201531 Msa0627770:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAATTTTTTTGTTAAAA+CGG - chr4_3:8201497-8201516 Msa0627770:three_prime_UTR 10.0%
!! AATAATAATAATACTAACAA+TGG + chr4_3:8201523-8201542 None:intergenic 10.0%
!!! GAAAAATTATTAAATTTTGA+GGG + chr4_3:8201681-8201700 None:intergenic 10.0%
!!! AGAAAAATTATTAAATTTTG+AGG + chr4_3:8201682-8201701 None:intergenic 10.0%
!!! TCTATATTTAGTTTTAAATT+TGG + chr4_3:8202051-8202070 None:intergenic 10.0%
!!! GTAATATATTTTTTTTATCA+AGG + chr4_3:8202397-8202416 None:intergenic 10.0%
!! TTAATAAAGTAATGAATTAT+TGG - chr4_3:8203093-8203112 Msa0627770:five_prime_UTR 10.0%
!!! TTTCAATTTTTTTGTTAAAA+CGG - chr4_3:8201497-8201516 Msa0627770:three_prime_UTR 10.0%
!! AATAATAATAATACTAACAA+TGG + chr4_3:8201523-8201542 None:intergenic 10.0%
!!! GAAAAATTATTAAATTTTGA+GGG + chr4_3:8201681-8201700 None:intergenic 10.0%
!!! AGAAAAATTATTAAATTTTG+AGG + chr4_3:8201682-8201701 None:intergenic 10.0%
!!! TCTATATTTAGTTTTAAATT+TGG + chr4_3:8202051-8202070 None:intergenic 10.0%
!!! GTAATATATTTTTTTTATCA+AGG + chr4_3:8202397-8202416 None:intergenic 10.0%
!! TTAATAAAGTAATGAATTAT+TGG - chr4_3:8203093-8203112 Msa0627770:five_prime_UTR 10.0%
!!! ATGTTAAATTTTAGAGAAAA+GGG + chr4_3:8201168-8201187 None:intergenic 15.0%
!!! AATTTAACATCTTTGTTATT+TGG - chr4_3:8201178-8201197 Msa0627770:three_prime_UTR 15.0%
!!! AAATTTAATAAACTTTTGCT+TGG - chr4_3:8201706-8201725 Msa0627770:three_prime_UTR 15.0%
!! ACACAAAATTTCATATTAAA+TGG + chr4_3:8202642-8202661 None:intergenic 15.0%
!!! ATGTTAAATTTTAGAGAAAA+GGG + chr4_3:8201168-8201187 None:intergenic 15.0%
!!! AATTTAACATCTTTGTTATT+TGG - chr4_3:8201178-8201197 Msa0627770:three_prime_UTR 15.0%
!!! AAATTTAATAAACTTTTGCT+TGG - chr4_3:8201706-8201725 Msa0627770:three_prime_UTR 15.0%
!! ACACAAAATTTCATATTAAA+TGG + chr4_3:8202642-8202661 None:intergenic 15.0%
!!! GATGTTAAATTTTAGAGAAA+AGG + chr4_3:8201169-8201188 None:intergenic 20.0%
!! TTGAACTATGAAAGAAAAAA+CGG + chr4_3:8201208-8201227 None:intergenic 20.0%
!!! TTTTTCTTTCATAGTTCAAT+TGG - chr4_3:8201209-8201228 Msa0627770:three_prime_UTR 20.0%
!!! TTTTCTTTCATAGTTCAATT+GGG - chr4_3:8201210-8201229 Msa0627770:three_prime_UTR 20.0%
!!! TAATTTTCTTGAGAAAATGA+AGG - chr4_3:8201251-8201270 Msa0627770:three_prime_UTR 20.0%
!!! ATTTTGAAACGTAATCAATA+AGG + chr4_3:8201914-8201933 None:intergenic 20.0%
!! ATTGATTACGTTTCAAAATT+TGG - chr4_3:8201915-8201934 Msa0627770:three_prime_UTR 20.0%
!! ATCCAACTTTCTTAATTTAA+TGG - chr4_3:8202418-8202437 Msa0627770:CDS 20.0%
!! TACCATTAAATTAAGAAAGT+TGG + chr4_3:8202423-8202442 None:intergenic 20.0%
!!! GATGTTAAATTTTAGAGAAA+AGG + chr4_3:8201169-8201188 None:intergenic 20.0%
!! TTGAACTATGAAAGAAAAAA+CGG + chr4_3:8201208-8201227 None:intergenic 20.0%
!!! TTTTTCTTTCATAGTTCAAT+TGG - chr4_3:8201209-8201228 Msa0627770:three_prime_UTR 20.0%
!!! TTTTCTTTCATAGTTCAATT+GGG - chr4_3:8201210-8201229 Msa0627770:three_prime_UTR 20.0%
!!! TAATTTTCTTGAGAAAATGA+AGG - chr4_3:8201251-8201270 Msa0627770:three_prime_UTR 20.0%
!!! ATTTTGAAACGTAATCAATA+AGG + chr4_3:8201914-8201933 None:intergenic 20.0%
!! ATTGATTACGTTTCAAAATT+TGG - chr4_3:8201915-8201934 Msa0627770:three_prime_UTR 20.0%
!! ATCCAACTTTCTTAATTTAA+TGG - chr4_3:8202418-8202437 Msa0627770:CDS 20.0%
!! TACCATTAAATTAAGAAAGT+TGG + chr4_3:8202423-8202442 None:intergenic 20.0%
! AATGAATAGAAGATTTGTCA+CGG + chr4_3:8201070-8201089 None:intergenic 25.0%
! GTGATCACAAATTATTGTTA+AGG + chr4_3:8201121-8201140 None:intergenic 25.0%
!!! AGATATGATTCACTGATTTT+AGG + chr4_3:8201299-8201318 None:intergenic 25.0%
! TCAATTGAAAACAACTTAGA+AGG - chr4_3:8201631-8201650 Msa0627770:intron 25.0%
! CAATTGAAAACAACTTAGAA+GGG - chr4_3:8201632-8201651 Msa0627770:intron 25.0%
! AATTGAAAACAACTTAGAAG+GGG - chr4_3:8201633-8201652 Msa0627770:intron 25.0%
! GAAGAAAGAAATAAATCCAA+AGG - chr4_3:8201845-8201864 Msa0627770:three_prime_UTR 25.0%
! AAAGATATGTGATTATCCTT+TGG + chr4_3:8201864-8201883 None:intergenic 25.0%
!! CGTTTATGTTATCCTTTTTA+AGG - chr4_3:8202123-8202142 Msa0627770:three_prime_UTR 25.0%
! GTGCAAAAAATAACATGATT+CGG + chr4_3:8202213-8202232 None:intergenic 25.0%
!! AAGCTCACACATTTTAAATA+CGG + chr4_3:8202361-8202380 None:intergenic 25.0%
! GTTTCATCAGAAATTAAACT+TGG - chr4_3:8202577-8202596 Msa0627770:intron 25.0%
!! CTCAACAATTTGTCATTTAT+CGG - chr4_3:8202606-8202625 Msa0627770:intron 25.0%
! TTTGTGTTTGAACTTTGAAT+TGG - chr4_3:8202655-8202674 Msa0627770:intron 25.0%
! CTTATTTGGACTGATATTAT+TGG - chr4_3:8203144-8203163 Msa0627770:five_prime_UTR 25.0%
! AATGAATAGAAGATTTGTCA+CGG + chr4_3:8201070-8201089 None:intergenic 25.0%
! GTGATCACAAATTATTGTTA+AGG + chr4_3:8201121-8201140 None:intergenic 25.0%
!!! AGATATGATTCACTGATTTT+AGG + chr4_3:8201299-8201318 None:intergenic 25.0%
! TCAATTGAAAACAACTTAGA+AGG - chr4_3:8201631-8201650 Msa0627770:intron 25.0%
! CAATTGAAAACAACTTAGAA+GGG - chr4_3:8201632-8201651 Msa0627770:intron 25.0%
! AATTGAAAACAACTTAGAAG+GGG - chr4_3:8201633-8201652 Msa0627770:intron 25.0%
! GAAGAAAGAAATAAATCCAA+AGG - chr4_3:8201845-8201864 Msa0627770:three_prime_UTR 25.0%
! AAAGATATGTGATTATCCTT+TGG + chr4_3:8201864-8201883 None:intergenic 25.0%
!! CGTTTATGTTATCCTTTTTA+AGG - chr4_3:8202123-8202142 Msa0627770:three_prime_UTR 25.0%
! GTGCAAAAAATAACATGATT+CGG + chr4_3:8202213-8202232 None:intergenic 25.0%
!! AAGCTCACACATTTTAAATA+CGG + chr4_3:8202361-8202380 None:intergenic 25.0%
! GTTTCATCAGAAATTAAACT+TGG - chr4_3:8202577-8202596 Msa0627770:intron 25.0%
!! CTCAACAATTTGTCATTTAT+CGG - chr4_3:8202606-8202625 Msa0627770:intron 25.0%
! TTTGTGTTTGAACTTTGAAT+TGG - chr4_3:8202655-8202674 Msa0627770:intron 25.0%
! CTTATTTGGACTGATATTAT+TGG - chr4_3:8203144-8203163 Msa0627770:five_prime_UTR 25.0%
!!! CTGGTTCTTTTGATTTTGAT+GGG - chr4_3:8201394-8201413 Msa0627770:three_prime_UTR 30.0%
!!! TGGTTCTTTTGATTTTGATG+GGG - chr4_3:8201395-8201414 Msa0627770:three_prime_UTR 30.0%
! GGATTTATTTCTTTCTTCCT+TGG + chr4_3:8201843-8201862 None:intergenic 30.0%
!!! TTTTTCTATGCGTCACTTTT+AGG + chr4_3:8202027-8202046 None:intergenic 30.0%
!! TCCTTTTTAAGGATATGCAA+TGG - chr4_3:8202134-8202153 Msa0627770:three_prime_UTR 30.0%
TTTAAGGATCTTATCCTCTA+AGG - chr4_3:8202159-8202178 Msa0627770:three_prime_UTR 30.0%
!!! ATGTTATTTTTTGCACTGCA+CGG - chr4_3:8202217-8202236 Msa0627770:three_prime_UTR 30.0%
ACAATGCATAATATATGCAG+AGG - chr4_3:8202312-8202331 Msa0627770:CDS 30.0%
! AGCTCACACATTTTAAATAC+GGG + chr4_3:8202360-8202379 None:intergenic 30.0%
!!! TTTTTTATCAAGGAGACTAG+TGG + chr4_3:8202387-8202406 None:intergenic 30.0%
CGATAAATGACAAATTGTTG+AGG + chr4_3:8202608-8202627 None:intergenic 30.0%
!!! CTGGTTCTTTTGATTTTGAT+GGG - chr4_3:8201394-8201413 Msa0627770:three_prime_UTR 30.0%
!!! TGGTTCTTTTGATTTTGATG+GGG - chr4_3:8201395-8201414 Msa0627770:three_prime_UTR 30.0%
! GGATTTATTTCTTTCTTCCT+TGG + chr4_3:8201843-8201862 None:intergenic 30.0%
!!! TTTTTCTATGCGTCACTTTT+AGG + chr4_3:8202027-8202046 None:intergenic 30.0%
!! TCCTTTTTAAGGATATGCAA+TGG - chr4_3:8202134-8202153 Msa0627770:three_prime_UTR 30.0%
TTTAAGGATCTTATCCTCTA+AGG - chr4_3:8202159-8202178 Msa0627770:three_prime_UTR 30.0%
!!! ATGTTATTTTTTGCACTGCA+CGG - chr4_3:8202217-8202236 Msa0627770:three_prime_UTR 30.0%
ACAATGCATAATATATGCAG+AGG - chr4_3:8202312-8202331 Msa0627770:CDS 30.0%
! AGCTCACACATTTTAAATAC+GGG + chr4_3:8202360-8202379 None:intergenic 30.0%
!!! TTTTTTATCAAGGAGACTAG+TGG + chr4_3:8202387-8202406 None:intergenic 30.0%
CGATAAATGACAAATTGTTG+AGG + chr4_3:8202608-8202627 None:intergenic 30.0%
TGTTAAGGAAGAGAAGAAAG+AGG + chr4_3:8201106-8201125 None:intergenic 35.0%
CTTTCATAGTTCAATTGGGT+TGG - chr4_3:8201214-8201233 Msa0627770:three_prime_UTR 35.0%
TGAGAAAATGAAGGAAAGTG+TGG - chr4_3:8201260-8201279 Msa0627770:three_prime_UTR 35.0%
! GAGAAAATGAAGGAAAGTGT+GGG - chr4_3:8201261-8201280 Msa0627770:three_prime_UTR 35.0%
!!! TCACTGATTTTAGGTGCAAA+GGG + chr4_3:8201290-8201309 None:intergenic 35.0%
!!! TTCACTGATTTTAGGTGCAA+AGG + chr4_3:8201291-8201310 None:intergenic 35.0%
GATTTCTACCAGAATGTTCT+TGG - chr4_3:8201351-8201370 Msa0627770:three_prime_UTR 35.0%
TTGGTTTCTTCCCAATAAGA+AGG - chr4_3:8201370-8201389 Msa0627770:three_prime_UTR 35.0%
CCATCAAAATCAAAAGAACC+AGG + chr4_3:8201396-8201415 None:intergenic 35.0%
!!! CCTGGTTCTTTTGATTTTGA+TGG - chr4_3:8201393-8201412 Msa0627770:three_prime_UTR 35.0%
GGATAATCACATATCTTTCC+AGG - chr4_3:8201866-8201885 Msa0627770:three_prime_UTR 35.0%
GTTTAGTGAAAAATCTGACC+TGG + chr4_3:8201887-8201906 None:intergenic 35.0%
TAAACGTGTCTATGATGACT+AGG + chr4_3:8202109-8202128 None:intergenic 35.0%
GCCATTGCATATCCTTAAAA+AGG + chr4_3:8202138-8202157 None:intergenic 35.0%
! AGGATATGCAATGGCTTTTA+AGG - chr4_3:8202143-8202162 Msa0627770:three_prime_UTR 35.0%
TGCATAATATATGCAGAGGT+AGG - chr4_3:8202316-8202335 Msa0627770:CDS 35.0%
GCATAATATATGCAGAGGTA+GGG - chr4_3:8202317-8202336 Msa0627770:CDS 35.0%
CATAATATATGCAGAGGTAG+GGG - chr4_3:8202318-8202337 Msa0627770:CDS 35.0%
!! CATTTTAAATACGGGGTATC+TGG + chr4_3:8202352-8202371 None:intergenic 35.0%
! GCTCACACATTTTAAATACG+GGG + chr4_3:8202359-8202378 None:intergenic 35.0%
TTGTTAACCATTCGATTCTC+AGG - chr4_3:8202494-8202513 Msa0627770:intron 35.0%
TGTTAACCATTCGATTCTCA+GGG - chr4_3:8202495-8202514 Msa0627770:intron 35.0%
ATCAGACTATACTTACCTCA+CGG + chr4_3:8202549-8202568 None:intergenic 35.0%
GGAGAAAAAGCTGAAAGAAA+TGG - chr4_3:8202709-8202728 Msa0627770:intron 35.0%
TCTCAATCATGAAACCATCT+GGG + chr4_3:8202802-8202821 None:intergenic 35.0%
ATCTCAATCATGAAACCATC+TGG + chr4_3:8202803-8202822 None:intergenic 35.0%
! TTTTCCCACACAAAGATGTT+TGG + chr4_3:8202967-8202986 None:intergenic 35.0%
AAACATCTTTGTGTGGGAAA+AGG - chr4_3:8202966-8202985 Msa0627770:exon 35.0%
TCGAAACAGAGTGACTTATT+TGG - chr4_3:8203130-8203149 Msa0627770:five_prime_UTR 35.0%
TGTTAAGGAAGAGAAGAAAG+AGG + chr4_3:8201106-8201125 None:intergenic 35.0%
CTTTCATAGTTCAATTGGGT+TGG - chr4_3:8201214-8201233 Msa0627770:three_prime_UTR 35.0%
TGAGAAAATGAAGGAAAGTG+TGG - chr4_3:8201260-8201279 Msa0627770:three_prime_UTR 35.0%
! GAGAAAATGAAGGAAAGTGT+GGG - chr4_3:8201261-8201280 Msa0627770:three_prime_UTR 35.0%
!!! TCACTGATTTTAGGTGCAAA+GGG + chr4_3:8201290-8201309 None:intergenic 35.0%
!!! TTCACTGATTTTAGGTGCAA+AGG + chr4_3:8201291-8201310 None:intergenic 35.0%
GATTTCTACCAGAATGTTCT+TGG - chr4_3:8201351-8201370 Msa0627770:three_prime_UTR 35.0%
TTGGTTTCTTCCCAATAAGA+AGG - chr4_3:8201370-8201389 Msa0627770:three_prime_UTR 35.0%
CCATCAAAATCAAAAGAACC+AGG + chr4_3:8201396-8201415 None:intergenic 35.0%
!!! CCTGGTTCTTTTGATTTTGA+TGG - chr4_3:8201393-8201412 Msa0627770:three_prime_UTR 35.0%
GGATAATCACATATCTTTCC+AGG - chr4_3:8201866-8201885 Msa0627770:three_prime_UTR 35.0%
GTTTAGTGAAAAATCTGACC+TGG + chr4_3:8201887-8201906 None:intergenic 35.0%
TAAACGTGTCTATGATGACT+AGG + chr4_3:8202109-8202128 None:intergenic 35.0%
GCCATTGCATATCCTTAAAA+AGG + chr4_3:8202138-8202157 None:intergenic 35.0%
! AGGATATGCAATGGCTTTTA+AGG - chr4_3:8202143-8202162 Msa0627770:three_prime_UTR 35.0%
TGCATAATATATGCAGAGGT+AGG - chr4_3:8202316-8202335 Msa0627770:CDS 35.0%
GCATAATATATGCAGAGGTA+GGG - chr4_3:8202317-8202336 Msa0627770:CDS 35.0%
CATAATATATGCAGAGGTAG+GGG - chr4_3:8202318-8202337 Msa0627770:CDS 35.0%
!! CATTTTAAATACGGGGTATC+TGG + chr4_3:8202352-8202371 None:intergenic 35.0%
! GCTCACACATTTTAAATACG+GGG + chr4_3:8202359-8202378 None:intergenic 35.0%
TTGTTAACCATTCGATTCTC+AGG - chr4_3:8202494-8202513 Msa0627770:intron 35.0%
TGTTAACCATTCGATTCTCA+GGG - chr4_3:8202495-8202514 Msa0627770:intron 35.0%
ATCAGACTATACTTACCTCA+CGG + chr4_3:8202549-8202568 None:intergenic 35.0%
GGAGAAAAAGCTGAAAGAAA+TGG - chr4_3:8202709-8202728 Msa0627770:intron 35.0%
TCTCAATCATGAAACCATCT+GGG + chr4_3:8202802-8202821 None:intergenic 35.0%
ATCTCAATCATGAAACCATC+TGG + chr4_3:8202803-8202822 None:intergenic 35.0%
! TTTTCCCACACAAAGATGTT+TGG + chr4_3:8202967-8202986 None:intergenic 35.0%
AAACATCTTTGTGTGGGAAA+AGG - chr4_3:8202966-8202985 Msa0627770:exon 35.0%
TCGAAACAGAGTGACTTATT+TGG - chr4_3:8203130-8203149 Msa0627770:five_prime_UTR 35.0%
GGAAGAAACCAAGAACATTC+TGG + chr4_3:8201362-8201381 None:intergenic 40.0%
AAGAACCAGGCCTTCTTATT+GGG + chr4_3:8201383-8201402 None:intergenic 40.0%
AAAGAACCAGGCCTTCTTAT+TGG + chr4_3:8201384-8201403 None:intergenic 40.0%
!!! CTTTTGATTTTGATGGGGCA+TGG - chr4_3:8201400-8201419 Msa0627770:three_prime_UTR 40.0%
GTATGTGTGACCAAATTTGC+AGG - chr4_3:8201760-8201779 Msa0627770:three_prime_UTR 40.0%
GACCAAATTTGCAGGCTATT+TGG - chr4_3:8201768-8201787 Msa0627770:three_prime_UTR 40.0%
AGCCAAATAGCCTGCAAATT+TGG + chr4_3:8201773-8201792 None:intergenic 40.0%
ATTTGCAGGCTATTTGGCTA+TGG - chr4_3:8201774-8201793 Msa0627770:three_prime_UTR 40.0%
AGGCTATTTGGCTATGGTAT+TGG - chr4_3:8201780-8201799 Msa0627770:three_prime_UTR 40.0%
! GCAATTTGAAGCAACCTTAG+AGG + chr4_3:8202176-8202195 None:intergenic 40.0%
AAGTCTACGTGAGCTTAACT+CGG - chr4_3:8202280-8202299 Msa0627770:CDS 40.0%
GCATCGTGCAATTGTAGTAA+AGG - chr4_3:8202445-8202464 Msa0627770:intron 40.0%
! TCTTTTCCCTGAGAATCGAA+TGG + chr4_3:8202504-8202523 None:intergenic 40.0%
! GGAAAAGAGCTCTGGTAATT+CGG - chr4_3:8202516-8202535 Msa0627770:intron 40.0%
TTATGTTCGAGCAAGAGTAG+AGG - chr4_3:8202739-8202758 Msa0627770:intron 40.0%
!! CTATTTTTCCATGACCCAGA+TGG - chr4_3:8202785-8202804 Msa0627770:intron 40.0%
CTTCCTGTGATTCCATTAGT+TGG - chr4_3:8202839-8202858 Msa0627770:CDS 40.0%
TCACCAACTAATGGAATCAC+AGG + chr4_3:8202845-8202864 None:intergenic 40.0%
! TGTGATTCCATTAGTTGGTG+AGG - chr4_3:8202844-8202863 Msa0627770:CDS 40.0%
TGTGACCAAACATCTTTGTG+TGG - chr4_3:8202959-8202978 Msa0627770:exon 40.0%
GTGACCAAACATCTTTGTGT+GGG - chr4_3:8202960-8202979 Msa0627770:exon 40.0%
GGAAGAAACCAAGAACATTC+TGG + chr4_3:8201362-8201381 None:intergenic 40.0%
AAGAACCAGGCCTTCTTATT+GGG + chr4_3:8201383-8201402 None:intergenic 40.0%
AAAGAACCAGGCCTTCTTAT+TGG + chr4_3:8201384-8201403 None:intergenic 40.0%
!!! CTTTTGATTTTGATGGGGCA+TGG - chr4_3:8201400-8201419 Msa0627770:three_prime_UTR 40.0%
GTATGTGTGACCAAATTTGC+AGG - chr4_3:8201760-8201779 Msa0627770:three_prime_UTR 40.0%
GACCAAATTTGCAGGCTATT+TGG - chr4_3:8201768-8201787 Msa0627770:three_prime_UTR 40.0%
AGCCAAATAGCCTGCAAATT+TGG + chr4_3:8201773-8201792 None:intergenic 40.0%
ATTTGCAGGCTATTTGGCTA+TGG - chr4_3:8201774-8201793 Msa0627770:three_prime_UTR 40.0%
AGGCTATTTGGCTATGGTAT+TGG - chr4_3:8201780-8201799 Msa0627770:three_prime_UTR 40.0%
! GCAATTTGAAGCAACCTTAG+AGG + chr4_3:8202176-8202195 None:intergenic 40.0%
AAGTCTACGTGAGCTTAACT+CGG - chr4_3:8202280-8202299 Msa0627770:CDS 40.0%
GCATCGTGCAATTGTAGTAA+AGG - chr4_3:8202445-8202464 Msa0627770:intron 40.0%
! TCTTTTCCCTGAGAATCGAA+TGG + chr4_3:8202504-8202523 None:intergenic 40.0%
! GGAAAAGAGCTCTGGTAATT+CGG - chr4_3:8202516-8202535 Msa0627770:intron 40.0%
TTATGTTCGAGCAAGAGTAG+AGG - chr4_3:8202739-8202758 Msa0627770:intron 40.0%
!! CTATTTTTCCATGACCCAGA+TGG - chr4_3:8202785-8202804 Msa0627770:intron 40.0%
CTTCCTGTGATTCCATTAGT+TGG - chr4_3:8202839-8202858 Msa0627770:CDS 40.0%
TCACCAACTAATGGAATCAC+AGG + chr4_3:8202845-8202864 None:intergenic 40.0%
! TGTGATTCCATTAGTTGGTG+AGG - chr4_3:8202844-8202863 Msa0627770:CDS 40.0%
TGTGACCAAACATCTTTGTG+TGG - chr4_3:8202959-8202978 Msa0627770:exon 40.0%
GTGACCAAACATCTTTGTGT+GGG - chr4_3:8202960-8202979 Msa0627770:exon 40.0%
TTCTTCCCAATAAGAAGGCC+TGG - chr4_3:8201375-8201394 Msa0627770:three_prime_UTR 45.0%
!! TCTTCCTTGGTAACGTCTCT+GGG + chr4_3:8201830-8201849 None:intergenic 45.0%
!! TTCTTCCTTGGTAACGTCTC+TGG + chr4_3:8201831-8201850 None:intergenic 45.0%
! TGAGCTTAACTCGGTTGGTA+GGG - chr4_3:8202289-8202308 Msa0627770:CDS 45.0%
ATTCTCAGGGAAAAGAGCTC+TGG - chr4_3:8202508-8202527 Msa0627770:intron 45.0%
TCATGAAACCATCTGGGTCA+TGG + chr4_3:8202796-8202815 None:intergenic 45.0%
CTTGCTACCTCACCAACTAA+TGG + chr4_3:8202854-8202873 None:intergenic 45.0%
TAAGTCACTCTGTTTCGAGC+AGG + chr4_3:8203128-8203147 None:intergenic 45.0%
TTCTTCCCAATAAGAAGGCC+TGG - chr4_3:8201375-8201394 Msa0627770:three_prime_UTR 45.0%
!! TCTTCCTTGGTAACGTCTCT+GGG + chr4_3:8201830-8201849 None:intergenic 45.0%
!! TTCTTCCTTGGTAACGTCTC+TGG + chr4_3:8201831-8201850 None:intergenic 45.0%
! TGAGCTTAACTCGGTTGGTA+GGG - chr4_3:8202289-8202308 Msa0627770:CDS 45.0%
ATTCTCAGGGAAAAGAGCTC+TGG - chr4_3:8202508-8202527 Msa0627770:intron 45.0%
TCATGAAACCATCTGGGTCA+TGG + chr4_3:8202796-8202815 None:intergenic 45.0%
CTTGCTACCTCACCAACTAA+TGG + chr4_3:8202854-8202873 None:intergenic 45.0%
TAAGTCACTCTGTTTCGAGC+AGG + chr4_3:8203128-8203147 None:intergenic 45.0%
!!! TTTAGTTTTAAATTTAAATT+TGG + chr4_3:8202079-8202098 None:intergenic 5.0%
!! TTAATAATATTTAACATATA+AGG + chr4_3:8203077-8203096 None:intergenic 5.0%
!!! TTTAGTTTTAAATTTAAATT+TGG + chr4_3:8202079-8202098 None:intergenic 5.0%
!! TTAATAATATTTAACATATA+AGG + chr4_3:8203077-8203096 None:intergenic 5.0%
AGAACCCAGAGACGTTACCA+AGG - chr4_3:8201823-8201842 Msa0627770:three_prime_UTR 50.0%
CTACGTGAGCTTAACTCGGT+TGG - chr4_3:8202284-8202303 Msa0627770:CDS 50.0%
GTGAGCTTAACTCGGTTGGT+AGG - chr4_3:8202288-8202307 Msa0627770:CDS 50.0%
TAATTCGGAGTTCGACCGTG+AGG - chr4_3:8202531-8202550 Msa0627770:intron 50.0%
TGATGTGCAGTGTGAGAGCA+TGG - chr4_3:8202679-8202698 Msa0627770:intron 50.0%
! GATGTGCAGTGTGAGAGCAT+GGG - chr4_3:8202680-8202699 Msa0627770:intron 50.0%
! ATGTGCAGTGTGAGAGCATG+GGG - chr4_3:8202681-8202700 Msa0627770:intron 50.0%
GTTCGAGCAAGAGTAGAGGA+AGG - chr4_3:8202743-8202762 Msa0627770:intron 50.0%
GAGCAAGAGTAGAGGAAGGT+GGG - chr4_3:8202747-8202766 Msa0627770:intron 50.0%
AGAGGAAGGTGGGATTGAAG+TGG - chr4_3:8202757-8202776 Msa0627770:intron 50.0%
AGAACCCAGAGACGTTACCA+AGG - chr4_3:8201823-8201842 Msa0627770:three_prime_UTR 50.0%
CTACGTGAGCTTAACTCGGT+TGG - chr4_3:8202284-8202303 Msa0627770:CDS 50.0%
GTGAGCTTAACTCGGTTGGT+AGG - chr4_3:8202288-8202307 Msa0627770:CDS 50.0%
TAATTCGGAGTTCGACCGTG+AGG - chr4_3:8202531-8202550 Msa0627770:intron 50.0%
TGATGTGCAGTGTGAGAGCA+TGG - chr4_3:8202679-8202698 Msa0627770:intron 50.0%
! GATGTGCAGTGTGAGAGCAT+GGG - chr4_3:8202680-8202699 Msa0627770:intron 50.0%
! ATGTGCAGTGTGAGAGCATG+GGG - chr4_3:8202681-8202700 Msa0627770:intron 50.0%
GTTCGAGCAAGAGTAGAGGA+AGG - chr4_3:8202743-8202762 Msa0627770:intron 50.0%
GAGCAAGAGTAGAGGAAGGT+GGG - chr4_3:8202747-8202766 Msa0627770:intron 50.0%
AGAGGAAGGTGGGATTGAAG+TGG - chr4_3:8202757-8202776 Msa0627770:intron 50.0%
! TGTGCAGTGTGAGAGCATGG+GGG - chr4_3:8202682-8202701 Msa0627770:intron 55.0%
CGAGCAAGAGTAGAGGAAGG+TGG - chr4_3:8202746-8202765 Msa0627770:intron 55.0%
! TGTGCAGTGTGAGAGCATGG+GGG - chr4_3:8202682-8202701 Msa0627770:intron 55.0%
CGAGCAAGAGTAGAGGAAGG+TGG - chr4_3:8202746-8202765 Msa0627770:intron 55.0%
! GTGTGAGAGCATGGGGGCAG+TGG - chr4_3:8202688-8202707 Msa0627770:intron 65.0%
! GTGTGAGAGCATGGGGGCAG+TGG - chr4_3:8202688-8202707 Msa0627770:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 8201067 8203168 8201067 ID=Msa0627770;Name=Msa0627770
chr4_3 mRNA 8201067 8203168 8201067 ID=Msa0627770-mRNA-1;Parent=Msa0627770;Name=Msa0627770-mRNA-1;_AED=0.04;_eAED=0.04;_QI=200|1|1|1|1|0.66|3|1076|109
chr4_3 exon 8201067 8201546 8201067 ID=Msa0627770-mRNA-1:exon:2370;Parent=Msa0627770-mRNA-1
chr4_3 exon 8201683 8202453 8201683 ID=Msa0627770-mRNA-1:exon:2369;Parent=Msa0627770-mRNA-1
chr4_3 exon 8202814 8203168 8202814 ID=Msa0627770-mRNA-1:exon:2368;Parent=Msa0627770-mRNA-1
chr4_3 five_prime_UTR 8202969 8203168 8202969 ID=Msa0627770-mRNA-1:five_prime_utr;Parent=Msa0627770-mRNA-1
chr4_3 CDS 8202814 8202968 8202814 ID=Msa0627770-mRNA-1:cds;Parent=Msa0627770-mRNA-1
chr4_3 CDS 8202279 8202453 8202279 ID=Msa0627770-mRNA-1:cds;Parent=Msa0627770-mRNA-1
chr4_3 three_prime_UTR 8201683 8202278 8201683 ID=Msa0627770-mRNA-1:three_prime_utr;Parent=Msa0627770-mRNA-1
chr4_3 three_prime_UTR 8201067 8201546 8201067 ID=Msa0627770-mRNA-1:three_prime_utr;Parent=Msa0627770-mRNA-1
Gene Sequence

>Msa0627770

ATGAAGGAAAGTGTGGGAAACCCTTTGCACCTAAAATCAGTGAATCATATCTCACTCATATGCAGATCAGTTGAAGAGTCAATAGATTTCTACCAGAATGTTCTTGGTTTCTTCCCAATAAGAAGGCCTGGTTCTTTTGATTTTGATGGGGCATGGCTATTTGGCTATGGTATTGGTATTCATTTGCTAGAAGCAGAGAACCCAGAGACGTTACCAAGGAAGAAAGAAATAAATCCAAAGGATAATCACATATCTTTCCAGGTCAGATTTTTCACTAAACTTAACCTTATTGATTACGTTTCAAAATTTGGAAGAAGAAAAATAATGTGA
Protein sequence

>Msa0627770

MKESVGNPLHLKSVNHISLICRSVEESIDFYQNVLGFFPIRRPGSFDFDGAWLFGYGIGIHLLEAENPETLPRKKEINPKDNHISFQVRFFTKLNLIDYVSKFGRRKIM*