Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0669330 | 0.805502 | 1.294474e-49 | -8.615850e-47 |
| Msa0669320 | Msa0669330 | 0.865083 | 7.636405e-65 | -8.615850e-47 |
| Msa0669330 | Msa1186060 | 0.810079 | 1.377543e-50 | -8.615850e-47 |
| Msa0157290 | Msa0669330 | 0.805897 | 1.069124e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0669330 | MtrunA17_Chr4g0008511 | 70.732 | 41 | 10 | 2 | 53 | 91 | 112 | 152 | 2.59e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 122 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCATCTTGAAAGAGATCT+TGG | 0.336659 | 4_4:+10991815 | None:intergenic |
| CACAAAATGTTAACTGTTGA+TGG | 0.348525 | 4_4:-10991952 | None:intergenic |
| TCGGTATTAGTTGTTCTTGT+GGG | 0.408647 | 4_4:-10990971 | Msa0669330:CDS |
| TTGTTCTTGTGGGAAATTGA+TGG | 0.420076 | 4_4:-10990961 | Msa0669330:CDS |
| ACTGGACAGTCTTCTCTTCA+TGG | 0.430946 | 4_4:-10991844 | Msa0669330:CDS |
| CAATAAATTGAAGTTCAAGT+TGG | 0.453085 | 4_4:-10991066 | Msa0669330:intron |
| GTCGGTATTAGTTGTTCTTG+TGG | 0.479823 | 4_4:-10990972 | Msa0669330:CDS |
| TCCGTATCCATAGCTGGTAC+TGG | 0.487289 | 4_4:-10991862 | Msa0669330:CDS |
| AAACTTGCAGTGGTGCTGTT+CGG | 0.499042 | 4_4:-10991912 | Msa0669330:CDS |
| AAATGTTAACTGTTGATGGC+TGG | 0.512332 | 4_4:-10991948 | Msa0669330:CDS |
| GTCATCTTGAAAGAGATCTT+GGG | 0.522854 | 4_4:+10991816 | None:intergenic |
| AAAGCTGCAGTAGGAGTGAT+TGG | 0.535807 | 4_4:-10991010 | Msa0669330:CDS |
| TCCGTGTCCGTATCCATAGC+TGG | 0.539219 | 4_4:-10991868 | Msa0669330:CDS |
| AATGTTAACTGTTGATGGCT+GGG | 0.545018 | 4_4:-10991947 | Msa0669330:CDS |
| CTTGAAAGAGATCTTGGGTA+TGG | 0.555810 | 4_4:+10991821 | None:intergenic |
| TCCAGTACCAGCTATGGATA+CGG | 0.567406 | 4_4:+10991861 | None:intergenic |
| AGACTGTCCAGTACCAGCTA+TGG | 0.570410 | 4_4:+10991855 | None:intergenic |
| TCATGTGTGAAAGCTGCAGT+AGG | 0.618233 | 4_4:-10991019 | Msa0669330:CDS |
| ACCAGCTATGGATACGGACA+CGG | 0.627014 | 4_4:+10991867 | None:intergenic |
| TGGACGATTCAGAGCTCATG+AGG | 0.667695 | 4_4:-10991046 | Msa0669330:CDS |
| GCTATGGATACGGACACGGA+AGG | 0.689390 | 4_4:+10991871 | None:intergenic |
| CTAGTTAGAGAAACTTGCAG+TGG | 0.691121 | 4_4:-10991922 | Msa0669330:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTTTAGAATTTATTTTGTC+TGG | - | chr4_4:10991119-10991138 | Msa0669330:intron | 20.0% |
| !!! | TTTATTTTGTCTGGTTTTAT+TGG | - | chr4_4:10991128-10991147 | Msa0669330:intron | 20.0% |
| !! | TTCAAAATTTCACTTAAGTA+CGG | + | chr4_4:10991706-10991725 | None:intergenic | 20.0% |
| !! | TAATCTTATAACTGAAGTAA+TGG | - | chr4_4:10991760-10991779 | Msa0669330:intron | 20.0% |
| !!! | GTTTTAGAATTTATTTTGTC+TGG | - | chr4_4:10991119-10991138 | Msa0669330:intron | 20.0% |
| !!! | TTTATTTTGTCTGGTTTTAT+TGG | - | chr4_4:10991128-10991147 | Msa0669330:intron | 20.0% |
| !! | TTCAAAATTTCACTTAAGTA+CGG | + | chr4_4:10991706-10991725 | None:intergenic | 20.0% |
| !! | TAATCTTATAACTGAAGTAA+TGG | - | chr4_4:10991760-10991779 | Msa0669330:intron | 20.0% |
| ! | TTCCATAAAACATGAATTGA+TGG | - | chr4_4:10991233-10991252 | Msa0669330:intron | 25.0% |
| !!! | TGATTAATCTTTGTTGTTGT+TGG | - | chr4_4:10991353-10991372 | Msa0669330:intron | 25.0% |
| !!! | GATTAATCTTTGTTGTTGTT+GGG | - | chr4_4:10991354-10991373 | Msa0669330:intron | 25.0% |
| ! | TTCGTATTATTATAGTCTGT+TGG | - | chr4_4:10991490-10991509 | Msa0669330:intron | 25.0% |
| ! | ATCCATTGGAAATAATTTGA+AGG | - | chr4_4:10991593-10991612 | Msa0669330:intron | 25.0% |
| ! | AAATAATTTGAAGGACCTAA+AGG | - | chr4_4:10991602-10991621 | Msa0669330:intron | 25.0% |
| ! | AATAATTTGAAGGACCTAAA+GGG | - | chr4_4:10991603-10991622 | Msa0669330:intron | 25.0% |
| !! | TGATGATCATAGGTTAATTT+TGG | - | chr4_4:10991656-10991675 | Msa0669330:intron | 25.0% |
| ! | AACTGTTTGAACTGAAAATT+GGG | - | chr4_4:10991724-10991743 | Msa0669330:intron | 25.0% |
| ! | CAATAAATTGAAGTTCAAGT+TGG | - | chr4_4:10991836-10991855 | Msa0669330:CDS | 25.0% |
| ! | TTCCATAAAACATGAATTGA+TGG | - | chr4_4:10991233-10991252 | Msa0669330:intron | 25.0% |
| !!! | TGATTAATCTTTGTTGTTGT+TGG | - | chr4_4:10991353-10991372 | Msa0669330:intron | 25.0% |
| !!! | GATTAATCTTTGTTGTTGTT+GGG | - | chr4_4:10991354-10991373 | Msa0669330:intron | 25.0% |
| ! | TTCGTATTATTATAGTCTGT+TGG | - | chr4_4:10991490-10991509 | Msa0669330:intron | 25.0% |
| ! | ATCCATTGGAAATAATTTGA+AGG | - | chr4_4:10991593-10991612 | Msa0669330:intron | 25.0% |
| ! | AAATAATTTGAAGGACCTAA+AGG | - | chr4_4:10991602-10991621 | Msa0669330:intron | 25.0% |
| ! | AATAATTTGAAGGACCTAAA+GGG | - | chr4_4:10991603-10991622 | Msa0669330:intron | 25.0% |
| !! | TGATGATCATAGGTTAATTT+TGG | - | chr4_4:10991656-10991675 | Msa0669330:intron | 25.0% |
| ! | AACTGTTTGAACTGAAAATT+GGG | - | chr4_4:10991724-10991743 | Msa0669330:intron | 25.0% |
| ! | CAATAAATTGAAGTTCAAGT+TGG | - | chr4_4:10991836-10991855 | Msa0669330:CDS | 25.0% |
| ! | GTCCATCAATTCATGTTTTA+TGG | + | chr4_4:10991238-10991257 | None:intergenic | 30.0% |
| ! | TTGTTGGGTTATTATGCTTT+TGG | - | chr4_4:10991369-10991388 | Msa0669330:intron | 30.0% |
| GCGACATATTCATGATTATT+GGG | - | chr4_4:10991391-10991410 | Msa0669330:intron | 30.0% | |
| TTATGCTGTTGATCTTAAGT+CGG | - | chr4_4:10991457-10991476 | Msa0669330:intron | 30.0% | |
| GTCCTTCAAATTATTTCCAA+TGG | + | chr4_4:10991598-10991617 | None:intergenic | 30.0% | |
| TTACATCTTTAACTCCCTTT+AGG | + | chr4_4:10991620-10991639 | None:intergenic | 30.0% | |
| !! | CTTTTGTCTTTGATGATCAT+AGG | - | chr4_4:10991646-10991665 | Msa0669330:intron | 30.0% |
| GAACTGTTTGAACTGAAAAT+TGG | - | chr4_4:10991723-10991742 | Msa0669330:intron | 30.0% | |
| ! | GTCCATCAATTCATGTTTTA+TGG | + | chr4_4:10991238-10991257 | None:intergenic | 30.0% |
| ! | TTGTTGGGTTATTATGCTTT+TGG | - | chr4_4:10991369-10991388 | Msa0669330:intron | 30.0% |
| GCGACATATTCATGATTATT+GGG | - | chr4_4:10991391-10991410 | Msa0669330:intron | 30.0% | |
| TTATGCTGTTGATCTTAAGT+CGG | - | chr4_4:10991457-10991476 | Msa0669330:intron | 30.0% | |
| GTCCTTCAAATTATTTCCAA+TGG | + | chr4_4:10991598-10991617 | None:intergenic | 30.0% | |
| TTACATCTTTAACTCCCTTT+AGG | + | chr4_4:10991620-10991639 | None:intergenic | 30.0% | |
| !! | CTTTTGTCTTTGATGATCAT+AGG | - | chr4_4:10991646-10991665 | Msa0669330:intron | 30.0% |
| GAACTGTTTGAACTGAAAAT+TGG | - | chr4_4:10991723-10991742 | Msa0669330:intron | 30.0% | |
| GTCATCTTGAAAGAGATCTT+GGG | + | chr4_4:10991089-10991108 | None:intergenic | 35.0% | |
| TGTCATCTTGAAAGAGATCT+TGG | + | chr4_4:10991090-10991109 | None:intergenic | 35.0% | |
| !!! | AGAGACAAAAGCATCACTTT+TGG | - | chr4_4:10991291-10991310 | Msa0669330:intron | 35.0% |
| GGCGACATATTCATGATTAT+TGG | - | chr4_4:10991390-10991409 | Msa0669330:intron | 35.0% | |
| !!! | ATGCTATTTTTGTATGCCAG+TGG | - | chr4_4:10991424-10991443 | Msa0669330:intron | 35.0% |
| ACTCATTTGACATGAAAGCA+GGG | - | chr4_4:10991565-10991584 | Msa0669330:intron | 35.0% | |
| TCACTTAAGTACGGTCAAAT+AGG | + | chr4_4:10991697-10991716 | None:intergenic | 35.0% | |
| AACTGAAAATTGGGAGCTAT+AGG | - | chr4_4:10991733-10991752 | Msa0669330:intron | 35.0% | |
| ! | ACTGAAGTAATGGTGTTTTG+TGG | - | chr4_4:10991770-10991789 | Msa0669330:intron | 35.0% |
| TATTGCAAAGTGAGTCACAA+GGG | + | chr4_4:10991821-10991840 | None:intergenic | 35.0% | |
| TTATTGCAAAGTGAGTCACA+AGG | + | chr4_4:10991822-10991841 | None:intergenic | 35.0% | |
| ! | TGGTGTTTGAGTACTTTTGT+CGG | - | chr4_4:10991912-10991931 | Msa0669330:CDS | 35.0% |
| TCGGTATTAGTTGTTCTTGT+GGG | - | chr4_4:10991931-10991950 | Msa0669330:CDS | 35.0% | |
| TTGTTCTTGTGGGAAATTGA+TGG | - | chr4_4:10991941-10991960 | Msa0669330:CDS | 35.0% | |
| GTCATCTTGAAAGAGATCTT+GGG | + | chr4_4:10991089-10991108 | None:intergenic | 35.0% | |
| TGTCATCTTGAAAGAGATCT+TGG | + | chr4_4:10991090-10991109 | None:intergenic | 35.0% | |
| !!! | AGAGACAAAAGCATCACTTT+TGG | - | chr4_4:10991291-10991310 | Msa0669330:intron | 35.0% |
| GGCGACATATTCATGATTAT+TGG | - | chr4_4:10991390-10991409 | Msa0669330:intron | 35.0% | |
| !!! | ATGCTATTTTTGTATGCCAG+TGG | - | chr4_4:10991424-10991443 | Msa0669330:intron | 35.0% |
| ACTCATTTGACATGAAAGCA+GGG | - | chr4_4:10991565-10991584 | Msa0669330:intron | 35.0% | |
| TCACTTAAGTACGGTCAAAT+AGG | + | chr4_4:10991697-10991716 | None:intergenic | 35.0% | |
| AACTGAAAATTGGGAGCTAT+AGG | - | chr4_4:10991733-10991752 | Msa0669330:intron | 35.0% | |
| ! | ACTGAAGTAATGGTGTTTTG+TGG | - | chr4_4:10991770-10991789 | Msa0669330:intron | 35.0% |
| TATTGCAAAGTGAGTCACAA+GGG | + | chr4_4:10991821-10991840 | None:intergenic | 35.0% | |
| TTATTGCAAAGTGAGTCACA+AGG | + | chr4_4:10991822-10991841 | None:intergenic | 35.0% | |
| ! | TGGTGTTTGAGTACTTTTGT+CGG | - | chr4_4:10991912-10991931 | Msa0669330:CDS | 35.0% |
| TCGGTATTAGTTGTTCTTGT+GGG | - | chr4_4:10991931-10991950 | Msa0669330:CDS | 35.0% | |
| TTGTTCTTGTGGGAAATTGA+TGG | - | chr4_4:10991941-10991960 | Msa0669330:CDS | 35.0% | |
| CTAGTTAGAGAAACTTGCAG+TGG | - | chr4_4:10990980-10990999 | Msa0669330:CDS | 40.0% | |
| CTTGAAAGAGATCTTGGGTA+TGG | + | chr4_4:10991084-10991103 | None:intergenic | 40.0% | |
| TGGTCATGATCTATGACAGA+TGG | - | chr4_4:10991180-10991199 | Msa0669330:intron | 40.0% | |
| GCATAATTGAACATCACCAC+TGG | + | chr4_4:10991443-10991462 | None:intergenic | 40.0% | |
| ATAGTCTGTTGGAGTCACTT+TGG | - | chr4_4:10991501-10991520 | Msa0669330:intron | 40.0% | |
| GACTCATTTGACATGAAAGC+AGG | - | chr4_4:10991564-10991583 | Msa0669330:intron | 40.0% | |
| GTCGGTATTAGTTGTTCTTG+TGG | - | chr4_4:10991930-10991949 | Msa0669330:CDS | 40.0% | |
| CTAGTTAGAGAAACTTGCAG+TGG | - | chr4_4:10990980-10990999 | Msa0669330:CDS | 40.0% | |
| CTTGAAAGAGATCTTGGGTA+TGG | + | chr4_4:10991084-10991103 | None:intergenic | 40.0% | |
| TGGTCATGATCTATGACAGA+TGG | - | chr4_4:10991180-10991199 | Msa0669330:intron | 40.0% | |
| GCATAATTGAACATCACCAC+TGG | + | chr4_4:10991443-10991462 | None:intergenic | 40.0% | |
| ATAGTCTGTTGGAGTCACTT+TGG | - | chr4_4:10991501-10991520 | Msa0669330:intron | 40.0% | |
| GACTCATTTGACATGAAAGC+AGG | - | chr4_4:10991564-10991583 | Msa0669330:intron | 40.0% | |
| GTCGGTATTAGTTGTTCTTG+TGG | - | chr4_4:10991930-10991949 | Msa0669330:CDS | 40.0% | |
| !! | AAACTTGCAGTGGTGCTGTT+CGG | - | chr4_4:10990990-10991009 | Msa0669330:CDS | 45.0% |
| TCCAGTACCAGCTATGGATA+CGG | + | chr4_4:10991044-10991063 | None:intergenic | 45.0% | |
| ACTGGACAGTCTTCTCTTCA+TGG | - | chr4_4:10991058-10991077 | Msa0669330:intron | 45.0% | |
| CCAATGATATCAGCTCAGCT+GGG | + | chr4_4:10991163-10991182 | None:intergenic | 45.0% | |
| ACCAATGATATCAGCTCAGC+TGG | + | chr4_4:10991164-10991183 | None:intergenic | 45.0% | |
| AAAGCAGGGACTCAATCCAT+TGG | - | chr4_4:10991579-10991598 | Msa0669330:intron | 45.0% | |
| TCATGTGTGAAAGCTGCAGT+AGG | - | chr4_4:10991883-10991902 | Msa0669330:CDS | 45.0% | |
| AAAGCTGCAGTAGGAGTGAT+TGG | - | chr4_4:10991892-10991911 | Msa0669330:CDS | 45.0% | |
| !! | AAACTTGCAGTGGTGCTGTT+CGG | - | chr4_4:10990990-10991009 | Msa0669330:CDS | 45.0% |
| TCCAGTACCAGCTATGGATA+CGG | + | chr4_4:10991044-10991063 | None:intergenic | 45.0% | |
| ACTGGACAGTCTTCTCTTCA+TGG | - | chr4_4:10991058-10991077 | Msa0669330:intron | 45.0% | |
| CCAATGATATCAGCTCAGCT+GGG | + | chr4_4:10991163-10991182 | None:intergenic | 45.0% | |
| ACCAATGATATCAGCTCAGC+TGG | + | chr4_4:10991164-10991183 | None:intergenic | 45.0% | |
| AAAGCAGGGACTCAATCCAT+TGG | - | chr4_4:10991579-10991598 | Msa0669330:intron | 45.0% | |
| TCATGTGTGAAAGCTGCAGT+AGG | - | chr4_4:10991883-10991902 | Msa0669330:CDS | 45.0% | |
| AAAGCTGCAGTAGGAGTGAT+TGG | - | chr4_4:10991892-10991911 | Msa0669330:CDS | 45.0% | |
| ACCAGCTATGGATACGGACA+CGG | + | chr4_4:10991038-10991057 | None:intergenic | 50.0% | |
| TCCGTATCCATAGCTGGTAC+TGG | - | chr4_4:10991040-10991059 | Msa0669330:CDS | 50.0% | |
| AGACTGTCCAGTACCAGCTA+TGG | + | chr4_4:10991050-10991069 | None:intergenic | 50.0% | |
| CCCAGCTGAGCTGATATCAT+TGG | - | chr4_4:10991160-10991179 | Msa0669330:intron | 50.0% | |
| ! | GTCTGTTGGAGTCACTTTGG+AGG | - | chr4_4:10991504-10991523 | Msa0669330:intron | 50.0% |
| AGTCACAAGGGTTGCACGAA+GGG | + | chr4_4:10991809-10991828 | None:intergenic | 50.0% | |
| TGGACGATTCAGAGCTCATG+AGG | - | chr4_4:10991856-10991875 | Msa0669330:CDS | 50.0% | |
| ACCAGCTATGGATACGGACA+CGG | + | chr4_4:10991038-10991057 | None:intergenic | 50.0% | |
| TCCGTATCCATAGCTGGTAC+TGG | - | chr4_4:10991040-10991059 | Msa0669330:CDS | 50.0% | |
| AGACTGTCCAGTACCAGCTA+TGG | + | chr4_4:10991050-10991069 | None:intergenic | 50.0% | |
| CCCAGCTGAGCTGATATCAT+TGG | - | chr4_4:10991160-10991179 | Msa0669330:intron | 50.0% | |
| ! | GTCTGTTGGAGTCACTTTGG+AGG | - | chr4_4:10991504-10991523 | Msa0669330:intron | 50.0% |
| AGTCACAAGGGTTGCACGAA+GGG | + | chr4_4:10991809-10991828 | None:intergenic | 50.0% | |
| TGGACGATTCAGAGCTCATG+AGG | - | chr4_4:10991856-10991875 | Msa0669330:CDS | 50.0% | |
| GCTATGGATACGGACACGGA+AGG | + | chr4_4:10991034-10991053 | None:intergenic | 55.0% | |
| TCCGTGTCCGTATCCATAGC+TGG | - | chr4_4:10991034-10991053 | Msa0669330:CDS | 55.0% | |
| GAGTCACAAGGGTTGCACGA+AGG | + | chr4_4:10991810-10991829 | None:intergenic | 55.0% | |
| GCTATGGATACGGACACGGA+AGG | + | chr4_4:10991034-10991053 | None:intergenic | 55.0% | |
| TCCGTGTCCGTATCCATAGC+TGG | - | chr4_4:10991034-10991053 | Msa0669330:CDS | 55.0% | |
| GAGTCACAAGGGTTGCACGA+AGG | + | chr4_4:10991810-10991829 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 10990956 | 10991968 | 10990956 | ID=Msa0669330;Name=Msa0669330 |
| chr4_4 | mRNA | 10990956 | 10991968 | 10990956 | ID=Msa0669330-mRNA-1;Parent=Msa0669330;Name=Msa0669330-mRNA-1;_AED=0.77;_eAED=0.77;_QI=0|0|0|0.5|0|0.5|2|0|91 |
| chr4_4 | exon | 10991813 | 10991968 | 10991813 | ID=Msa0669330-mRNA-1:exon:3332;Parent=Msa0669330-mRNA-1 |
| chr4_4 | exon | 10990956 | 10991075 | 10990956 | ID=Msa0669330-mRNA-1:exon:3331;Parent=Msa0669330-mRNA-1 |
| chr4_4 | CDS | 10991813 | 10991968 | 10991813 | ID=Msa0669330-mRNA-1:cds;Parent=Msa0669330-mRNA-1 |
| chr4_4 | CDS | 10990956 | 10991075 | 10990956 | ID=Msa0669330-mRNA-1:cds;Parent=Msa0669330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
