AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0671230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0671230 MtrunA17_Chr4g0010511 96.950 623 19 0 1 623 1 623 0.0 1247
Msa0671230 MtrunA17_Chr3g0087291 68.860 623 134 9 1 623 1 563 0.0 799
Msa0671230 MtrunA17_Chr4g0070551 36.795 443 256 9 189 616 184 617 6.96e-87 283
Msa0671230 MtrunA17_Chr3g0129981 36.735 441 242 11 199 616 85 511 1.03e-80 264
Msa0671230 MtrunA17_Chr5g0426591 34.286 490 267 12 164 616 40 511 7.12e-80 262
Msa0671230 MtrunA17_Chr5g0448581 36.427 431 255 9 196 619 84 502 3.21e-77 256
Msa0671230 MtrunA17_Chr4g0056061 39.231 390 217 9 236 616 111 489 9.50e-77 254
Msa0671230 MtrunA17_Chr5g0399311 37.875 433 241 9 202 622 95 511 1.15e-76 253
Msa0671230 MtrunA17_Chr3g0132371 36.717 463 240 13 184 616 99 538 4.84e-76 252
Msa0671230 MtrunA17_Chr3g0143821 35.398 452 255 8 191 619 58 495 5.97e-76 253
Msa0671230 MtrunA17_Chr4g0042711 36.409 401 236 8 236 622 100 495 2.38e-75 249
Msa0671230 MtrunA17_Chr4g0008451 33.945 436 268 8 196 620 199 625 2.58e-73 248
Msa0671230 MtrunA17_Chr5g0412251 34.581 454 246 10 202 616 60 501 8.47e-73 243
Msa0671230 MtrunA17_Chr5g0436321 33.126 483 272 10 179 616 74 550 2.45e-72 243
Msa0671230 MtrunA17_Chr5g0417871 35.802 405 235 9 236 623 88 484 5.22e-71 238
Msa0671230 MtrunA17_Chr4g0061781 37.634 372 220 6 236 598 119 487 8.02e-71 238
Msa0671230 MtrunA17_Chr6g0455571 37.333 375 219 8 236 599 118 487 1.38e-70 237
Msa0671230 MtrunA17_Chr4g0043911 34.014 441 255 11 204 622 127 553 1.39e-70 238
Msa0671230 MtrunA17_Chr3g0132951 34.305 446 240 10 211 616 83 515 4.50e-70 236
Msa0671230 MtrunA17_Chr1g0204121 32.944 428 266 9 202 619 61 477 1.93e-69 234
Msa0671230 MtrunA17_Chr1g0160781 33.925 451 258 11 191 617 113 547 3.77e-69 234
Msa0671230 MtrunA17_Chr2g0307971 36.685 368 219 6 236 593 118 481 3.91e-69 233
Msa0671230 MtrunA17_Chr3g0123521 32.883 444 261 12 199 616 115 547 3.92e-68 231
Msa0671230 MtrunA17_Chr5g0424241 32.000 450 276 9 191 616 116 559 3.64e-65 224
Msa0671230 MtrunA17_Chr3g0077321 33.333 396 248 6 236 616 150 544 1.77e-63 219
Msa0671230 MtrunA17_Chr1g0204141 31.019 432 272 11 202 619 61 480 1.49e-59 207
Msa0671230 MtrunA17_Chr2g0284071 29.114 474 265 16 199 616 182 640 2.02e-49 182
Msa0671230 MtrunA17_Chr4g0075311 30.917 469 262 17 199 616 134 591 8.53e-47 174
Msa0671230 MtrunA17_Chr4g0003561 27.318 399 234 13 236 616 78 438 3.12e-31 126
Msa0671230 MtrunA17_Chr7g0220381 29.655 290 189 6 236 510 101 390 4.54e-30 125
Msa0671230 MtrunA17_Chr1g0146501 27.273 330 222 7 196 510 56 382 3.97e-26 113
Msa0671230 MtrunA17_Chr3g0127811 27.541 305 200 7 217 510 48 342 5.29e-23 102
Msa0671230 MtrunA17_Chr2g0298881 24.603 378 220 13 213 569 20 353 1.51e-21 97.1
Msa0671230 MtrunA17_Chr4g0002361 24.737 380 219 12 194 561 65 389 6.06e-21 95.9
Msa0671230 MtrunA17_Chr7g0240241 34.921 126 64 2 389 504 2 119 1.04e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0671230 AT2G44500.1 63.235 612 156 9 11 618 17 563 0.0 751
Msa0671230 AT3G07900.1 59.806 617 190 14 10 621 14 577 0.0 681
Msa0671230 AT5G63390.1 55.579 484 204 3 141 623 82 555 0.0 556
Msa0671230 AT2G44500.2 56.484 455 129 9 11 461 17 406 1.54e-163 474
Msa0671230 AT5G35570.1 36.878 442 247 11 198 622 220 646 1.65e-81 270
Msa0671230 AT1G76270.1 36.136 440 248 10 194 619 69 489 1.54e-79 263
Msa0671230 AT1G20550.1 36.279 430 261 10 194 619 72 492 1.01e-78 260
Msa0671230 AT4G24530.1 35.590 458 242 11 199 616 72 516 1.32e-77 256
Msa0671230 AT5G64600.1 36.199 442 245 11 199 617 88 515 1.82e-76 253
Msa0671230 AT3G54100.1 34.112 428 266 7 202 620 195 615 3.37e-76 255
Msa0671230 AT1G22460.1 35.845 438 244 10 202 616 134 557 7.40e-75 250
Msa0671230 AT5G01100.1 34.101 434 266 8 202 623 190 615 5.13e-74 249
Msa0671230 AT2G37980.1 33.103 435 261 9 202 620 199 619 3.64e-73 247
Msa0671230 AT2G03280.1 38.070 373 215 7 237 598 92 459 4.19e-73 243
Msa0671230 AT5G65470.1 33.404 470 260 10 189 617 46 503 6.81e-73 243
Msa0671230 AT4G38390.1 37.250 400 220 8 235 619 125 508 2.92e-72 243
Msa0671230 AT1G14020.1 35.930 398 234 8 237 619 95 486 9.96e-70 234
Msa0671230 AT5G15740.1 36.193 373 224 6 236 598 109 477 2.34e-69 234
Msa0671230 AT1G14970.2 33.333 435 254 9 204 616 12 432 7.48e-69 231
Msa0671230 AT2G03280.2 35.500 400 215 8 237 598 92 486 2.15e-67 229
Msa0671230 AT1G14970.1 33.333 435 254 9 204 616 132 552 5.09e-67 229
Msa0671230 AT4G16650.2 40.360 389 216 11 236 617 125 504 5.11e-67 229
Msa0671230 AT4G16650.1 40.360 389 216 11 236 617 125 504 5.11e-67 229
Msa0671230 AT1G14970.3 33.738 412 245 7 226 616 2 406 1.10e-66 224
Msa0671230 AT3G02250.1 34.828 379 221 6 236 598 109 477 1.34e-65 224
Msa0671230 AT1G04910.1 31.542 428 266 11 202 616 61 474 4.49e-65 223
Msa0671230 AT3G26370.1 33.668 398 244 8 236 616 161 555 4.64e-65 224
Msa0671230 AT1G38065.2 32.850 414 254 10 224 616 18 428 9.27e-63 215
Msa0671230 AT1G35510.1 32.118 439 262 9 202 618 134 558 9.84e-63 218
Msa0671230 AT1G38065.1 33.085 402 246 9 236 616 39 438 1.84e-62 214
Msa0671230 AT2G01480.1 32.854 417 252 8 220 615 141 550 4.22e-62 216
Msa0671230 AT1G38131.1 32.850 414 254 10 224 616 147 557 1.17e-61 215
Msa0671230 AT2G01480.2 33.867 375 225 5 220 575 141 511 3.79e-60 209
Msa0671230 AT1G62330.1 29.193 483 259 15 198 618 190 651 1.94e-54 197
Msa0671230 AT1G11990.1 30.737 475 288 16 167 617 132 589 3.38e-53 192
Msa0671230 AT1G11990.2 29.106 481 267 15 198 617 144 611 2.02e-47 176
Msa0671230 AT1G29200.4 32.613 463 235 18 212 616 50 493 3.11e-47 174
Msa0671230 AT1G29200.1 32.613 463 235 18 212 616 50 493 3.11e-47 174
Msa0671230 AT1G29200.2 32.613 463 235 18 212 616 166 609 9.55e-47 174
Msa0671230 AT1G29200.3 33.333 432 222 16 238 616 1 419 1.18e-45 167
Msa0671230 AT3G30300.2 32.759 290 180 6 236 510 103 392 7.13e-35 140
Msa0671230 AT3G30300.1 32.759 290 180 6 236 510 103 392 7.13e-35 140
Msa0671230 AT1G52630.1 24.249 433 281 13 195 616 39 435 2.67e-29 121
Msa0671230 AT3G26370.2 32.967 182 108 4 236 404 161 341 1.07e-28 119
Msa0671230 AT3G03810.2 30.375 293 183 7 236 510 92 381 6.24e-27 116
Msa0671230 AT3G03810.1 30.375 293 183 7 236 510 92 381 6.24e-27 116
Msa0671230 AT1G52630.2 24.802 379 241 12 249 616 2 347 2.77e-25 108

Find 176 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTATGGTGGTTGTTTC+TGG 0.090360 4_4:+14234329 Msa0671230:CDS
GCTTAATATGTATGCAATTT+AGG 0.118952 4_4:+14237283 Msa0671230:three_prime_UTR
TGTATGTAACATCCCTTTAA+TGG 0.185995 4_4:-14237317 None:intergenic
ACCAAACCCATTTGAGATTA+AGG 0.186427 4_4:-14233922 None:intergenic
GCCATCTCATGGAGGAAATA+TGG 0.218103 4_4:+14236329 Msa0671230:CDS
GTTGTTATTGCTAGGATTCT+TGG 0.223923 4_4:+14234389 Msa0671230:CDS
ATGACATTAACTTGCAAAAT+AGG 0.227744 4_4:-14234428 None:intergenic
TCTTCACCACCTTAACAAAA+TGG 0.248210 4_4:+14233772 Msa0671230:CDS
TATAGGTTGCTTAAAGCTTT+AGG 0.261962 4_4:+14236102 Msa0671230:intron
AGAGAAGCTTTCTTCGAAAA+TGG 0.262334 4_4:-14236258 None:intergenic
CATACATATTAAGCATATTA+AGG 0.267084 4_4:-14237274 None:intergenic
GGTTTCCTGTGATCCATTAA+AGG 0.271188 4_4:+14237304 Msa0671230:three_prime_UTR
AGAATCAATGTCAAAGCTTT+TGG 0.282700 4_4:+14237064 Msa0671230:three_prime_UTR
GAGGGAAGAATTCAGAAAAT+AGG 0.283536 4_4:-14236890 None:intergenic
GACCAGCCATCTTTCTTTCA+TGG 0.284935 4_4:-14235689 None:intergenic
ACTGCTTGGAGGCTACTTTC+AGG 0.288500 4_4:+14237173 Msa0671230:three_prime_UTR
GTTTCCACATCTTTATAATA+AGG 0.294769 4_4:+14236206 Msa0671230:CDS
TTTGCAAGTTAATGTCATTT+GGG 0.301248 4_4:+14234433 Msa0671230:CDS
AAAAGATGCAAGAATTTATT+GGG 0.322642 4_4:+14236131 Msa0671230:CDS
AAATATGGGCCATGCGCTTC+AGG 0.329180 4_4:+14236344 Msa0671230:CDS
TCTTTCTCTTTCATCTTCAA+TGG 0.331525 4_4:+14233605 Msa0671230:exon
GAAAAGCCCTTAATCTCAAA+TGG 0.333986 4_4:+14233915 Msa0671230:CDS
CCAAACCCATTTGAGATTAA+GGG 0.341142 4_4:-14233921 None:intergenic
CAAAACCCTTCAATCTCAAA+TGG 0.341182 4_4:+14234032 Msa0671230:CDS
AAAGAGAAAGAAAGAGTATT+GGG 0.342315 4_4:-14233593 None:intergenic
CCCTTAATCTCAAATGGGTT+TGG 0.349127 4_4:+14233921 Msa0671230:CDS
CTCAGAAACTATGTAATCTA+TGG 0.350072 4_4:-14236289 None:intergenic
TGGAAGCAACCAGATGGGTT+AGG 0.362833 4_4:+14234233 Msa0671230:CDS
TAGGATTCTTGGTGCTGCTT+TGG 0.371467 4_4:+14234400 Msa0671230:CDS
ATGCTGTTAAACTCTGCTTC+AGG 0.374694 4_4:-14236913 None:intergenic
CTACTTTACATATACAGATA+TGG 0.381841 4_4:+14237518 Msa0671230:three_prime_UTR
AGGGAAGAATTCAGAAAATA+GGG 0.381917 4_4:-14236889 None:intergenic
TTGAATAGAAGAAGATGAAA+TGG 0.388256 4_4:-14233691 None:intergenic
TGGTTCAAAATCTGAACTTA+AGG 0.388422 4_4:+14233941 Msa0671230:CDS
TTCGAAGAAAGCTTCTCTAA+TGG 0.388722 4_4:+14236263 Msa0671230:CDS
GTTAGGGTATAAGCCTTGTT+TGG 0.390330 4_4:+14234250 Msa0671230:CDS
GAATTGCATCAGAATTCATT+AGG 0.395674 4_4:+14236943 Msa0671230:CDS
GAAAGAGAAAGAAAGAGTAT+TGG 0.397924 4_4:-14233594 None:intergenic
TGTGGAAACTCATGGATTAA+TGG 0.400222 4_4:-14236192 None:intergenic
CAACAAAGCTTTGTTGTGTT+GGG 0.401012 4_4:-14233508 None:intergenic
GTTTCCTGTGATCCATTAAA+GGG 0.401903 4_4:+14237305 Msa0671230:three_prime_UTR
GCGAAGCCAGTTCAAGAACT+TGG 0.408751 4_4:+14235483 Msa0671230:CDS
TTGTTTCCAAGTTCTTGAAC+TGG 0.412623 4_4:-14235489 None:intergenic
GGAGAGAGAAAGAGAGAGGA+AGG 0.413292 4_4:-14233426 None:intergenic
GAAGAATGGTAGAGAGAGAA+AGG 0.413637 4_4:-14233404 None:intergenic
TCTTTCTATAGATCTTGTAT+AGG 0.417439 4_4:+14237359 Msa0671230:three_prime_UTR
ACCTTGCTAGTCCTGAGTTC+CGG 0.418631 4_4:-14236970 None:intergenic
GTGGAAGCAAGTAGTACTTT+TGG 0.425786 4_4:+14237121 Msa0671230:three_prime_UTR
TGACTTGAAGGATGAAGAAA+AGG 0.428102 4_4:+14234184 Msa0671230:CDS
GAATAGAAAGAGGTATCTTA+TGG 0.428527 4_4:+14234316 Msa0671230:CDS
AGGGGAGGGCTTCCCTCCAC+TGG 0.428966 4_4:-14234970 None:intergenic
AAAGGGTGAAGGAGTTGAAA+AGG 0.430675 4_4:+14234202 Msa0671230:CDS
GGAAGCAACCAGATGGGTTA+GGG 0.436626 4_4:+14234234 Msa0671230:CDS
ACAACAAAGCTTTGTTGTGT+TGG 0.439559 4_4:-14233509 None:intergenic
TCAAACCCATTTGAGATTGA+AGG 0.439951 4_4:-14234038 None:intergenic
ATGCAAGAATTTATTGGGCC+GGG 0.443277 4_4:+14236136 Msa0671230:CDS
GTATTTCTTGTGTCCTGCAT+AGG 0.451809 4_4:-14236839 None:intergenic
GGCTTATACCCTAACCCATC+TGG 0.457469 4_4:-14234242 None:intergenic
GTTATTCCTTCCAGGGTCAC+AGG 0.458415 4_4:+14236815 Msa0671230:intron
ATAGAAAATGGAATAATAAG+AGG 0.458820 4_4:-14233447 None:intergenic
AAAGAGAGAGGAAGGAAGAA+TGG 0.459758 4_4:-14233418 None:intergenic
GAGTTGAAAAGGGTGAGTTT+TGG 0.461295 4_4:+14234213 Msa0671230:CDS
TGAAACCACGCGCACATCAT+TGG 0.464474 4_4:-14234917 None:intergenic
TATAAAGATGTGGAAACTCA+TGG 0.469538 4_4:-14236200 None:intergenic
AAGGAAGATCTTGCATTGCC+TGG 0.471250 4_4:+14236225 Msa0671230:CDS
TCCTAGTGGAGTTGACTTGA+AGG 0.472681 4_4:+14234172 Msa0671230:CDS
CCCACCCAATGGTTGTCCCC+CGG 0.473532 4_4:-14236154 None:intergenic
AAAACCCTTCAATCTCAAAT+GGG 0.474207 4_4:+14234033 Msa0671230:CDS
TGCTGTTAAACTCTGCTTCA+GGG 0.476049 4_4:-14236912 None:intergenic
ATCCCTCGAAAACTGCTTGG+AGG 0.476778 4_4:+14237162 Msa0671230:three_prime_UTR
CTTATGGTGGTTGTTTCTGG+TGG 0.479116 4_4:+14234332 Msa0671230:CDS
CCATCTCATGGAGGAAATAT+GGG 0.480168 4_4:+14236330 Msa0671230:CDS
AAGGGTGAAGGAGTTGAAAA+GGG 0.483113 4_4:+14234203 Msa0671230:CDS
TAGCGCGAACGGATCCAACT+TGG 0.483932 4_4:-14235003 None:intergenic
AAAAGCCCTTAATCTCAAAT+GGG 0.485351 4_4:+14233916 Msa0671230:CDS
GTGAGGACTGGTTGTCTACC+CGG 0.488967 4_4:+14235582 Msa0671230:CDS
CCTTGCTCTTCATCTAAGAA+TGG 0.490124 4_4:+14235545 Msa0671230:CDS
TTATGGTGGTTGTTTCTGGT+GGG 0.492513 4_4:+14234333 Msa0671230:CDS
GATGCAAGAATTTATTGGGC+CGG 0.492621 4_4:+14236135 Msa0671230:CDS
TTTATCCCTCGAAAACTGCT+TGG 0.492661 4_4:+14237159 Msa0671230:three_prime_UTR
AAGGATGTGTGGGTGAGGAC+TGG 0.493609 4_4:+14235570 Msa0671230:CDS
ATCAAACTCAGGACTTAGAC+CGG 0.494512 4_4:-14235601 None:intergenic
CAAACCCATTTGAGATTGAA+GGG 0.494557 4_4:-14234037 None:intergenic
GATCTTCCTTTCAAGAGCTC+CGG 0.494862 4_4:-14235654 None:intergenic
TGCAAGAATTTATTGGGCCG+GGG 0.505342 4_4:+14236137 Msa0671230:CDS
GAAAATGGTTCTAGTTCTCC+AGG 0.505643 4_4:-14236243 None:intergenic
CCCTTCATGTCACTCCAAGT+TGG 0.506916 4_4:+14234989 Msa0671230:CDS
AACTCAGGACTAGCAAGGTC+GGG 0.506943 4_4:+14236974 Msa0671230:CDS
TTGCACATGCACTCTGGGAC+AGG 0.510427 4_4:-14237015 None:intergenic
CCTTTGCAGTTCAACAGAGA+AGG 0.513180 4_4:+14235276 Msa0671230:intron
TCTTTGTCCCTTGACTGCCA+TGG 0.516067 4_4:+14235710 Msa0671230:CDS
AACATTACATTAGCAATCAA+AGG 0.526009 4_4:-14234137 None:intergenic
GATTACAGAAGAGAAAGTGA+TGG 0.526049 4_4:+14234284 Msa0671230:CDS
TCCAACTTGGAGTGACATGA+AGG 0.527976 4_4:-14234990 None:intergenic
ATGAGATTGTAAAGAATGAG+AGG 0.529082 4_4:+14235622 Msa0671230:CDS
TCTTCATCTAAGAATGGAAA+AGG 0.529212 4_4:+14235551 Msa0671230:CDS
TAGTTTGTAGATAATGAAGA+TGG 0.530544 4_4:-14237498 None:intergenic
ATGTCATTTGGGGAGATGAA+AGG 0.532142 4_4:+14234444 Msa0671230:CDS
GGTAACATCCATGGCAGTCA+AGG 0.535475 4_4:-14235718 None:intergenic
TCTTAGATGAAGAGCAAGGT+AGG 0.537764 4_4:-14235541 None:intergenic
ATTACAGAAGAGAAAGTGAT+GGG 0.538851 4_4:+14234285 Msa0671230:CDS
TAATGTAATGTTGCAAGCAA+AGG 0.539312 4_4:+14234148 Msa0671230:CDS
GAATGAGAGGAGAGAACGAC+CGG 0.540471 4_4:+14235635 Msa0671230:CDS
CGAAGCTTCTGAAGATCTGA+AGG 0.542834 4_4:-14235345 None:intergenic
GTAGAGAGAGAAAGGGCGGC+GGG 0.543504 4_4:-14233396 None:intergenic
TATTTACCTGGTAACATCCA+TGG 0.544732 4_4:-14235727 None:intergenic
ACATCCCTTTAATGGATCAC+AGG 0.545504 4_4:-14237309 None:intergenic
CAGGGTCACAGGGCCTATGC+AGG 0.546366 4_4:+14236826 Msa0671230:intron
TCAGAATTCATTAGGACAGC+CGG 0.547453 4_4:+14236951 Msa0671230:CDS
AAGAGGAGAGAGAAAGAGAG+AGG 0.547573 4_4:-14233430 None:intergenic
TGGAAAAGGATGTGTGGGTG+AGG 0.548660 4_4:+14235565 Msa0671230:CDS
GAGTCGTTGCACATGCACTC+TGG 0.553547 4_4:-14237021 None:intergenic
AAGAATGGTAGAGAGAGAAA+GGG 0.553930 4_4:-14233403 None:intergenic
CATTAGGACAGCCGGAACTC+AGG 0.555136 4_4:+14236959 Msa0671230:CDS
TTGATGCTGTTGTTATTGCT+AGG 0.555554 4_4:+14234381 Msa0671230:CDS
GAACGACCGGAGCTCTTGAA+AGG 0.557470 4_4:+14235648 Msa0671230:CDS
TTTACAATCTCATCAAACTC+AGG 0.560411 4_4:-14235612 None:intergenic
GTAGATAATGAAGATGGAGC+TGG 0.560656 4_4:-14237492 None:intergenic
TACCATGAAAGAAAGATGGC+TGG 0.562902 4_4:+14235687 Msa0671230:CDS
CCCATATTTCCTCCATGAGA+TGG 0.566943 4_4:-14236330 None:intergenic
ATGGAATTGATTATTTGTGA+AGG 0.568945 4_4:-14233672 None:intergenic
AAAGCTTCTTTCCCACCCAA+TGG 0.570016 4_4:-14236165 None:intergenic
TTTGATTCAAAATCTGAACT+TGG 0.571345 4_4:+14234056 Msa0671230:CDS
TCTTCAGAAGCTTCGATGCA+AGG 0.571820 4_4:+14235353 Msa0671230:CDS
TTCAACATTTGTAGCACATG+GGG 0.574252 4_4:-14233895 None:intergenic
AGTCGTTGCACATGCACTCT+GGG 0.575723 4_4:-14237020 None:intergenic
TTTATTCAGATATATGTACA+AGG 0.576413 4_4:-14237423 None:intergenic
TGTCAGCCACTCCTCTCCGC+AGG 0.576492 4_4:+14237236 Msa0671230:three_prime_UTR
AAAAGAAGAACAATCTTGAA+GGG 0.576810 4_4:-14233800 None:intergenic
GACTTACCATGAAAGAAAGA+TGG 0.579868 4_4:+14235683 Msa0671230:CDS
TTTGTCATCAAGTGTGTGGA+TGG 0.580452 4_4:-14234946 None:intergenic
GAACTCAGGACTAGCAAGGT+CGG 0.582708 4_4:+14236973 Msa0671230:CDS
TTATTCCTTCCAGGGTCACA+GGG 0.583835 4_4:+14236816 Msa0671230:intron
AAGGATGAAGAAAAGGGTGA+AGG 0.588524 4_4:+14234191 Msa0671230:CDS
CTTGGATTCAAGGCTATCAA+AGG 0.589401 4_4:+14235317 Msa0671230:CDS
TCTTCCTTATTATAAAGATG+TGG 0.591443 4_4:-14236210 None:intergenic
TGACTGCCATGGATGTTACC+AGG 0.594053 4_4:+14235721 Msa0671230:CDS
TTTCAACATTTGTAGCACAT+GGG 0.594469 4_4:-14233896 None:intergenic
GACTTGAAGGATGAAGAAAA+GGG 0.596632 4_4:+14234185 Msa0671230:CDS
TCCTTCAAGTCAACTCCACT+AGG 0.600882 4_4:-14234173 None:intergenic
GGTAGAGAGAGAAAGGGCGG+CGG 0.602337 4_4:-14233397 None:intergenic
GCAAGAATTTATTGGGCCGG+GGG 0.602690 4_4:+14236138 Msa0671230:CDS
CCAACTTGGAGTGACATGAA+GGG 0.602705 4_4:-14234989 None:intergenic
TTGCAAGTTAATGTCATTTG+GGG 0.605474 4_4:+14234434 Msa0671230:CDS
CAACTTGGAGTGACATGAAG+GGG 0.608175 4_4:-14234988 None:intergenic
GTTAAACTCTGCTTCAGGGA+GGG 0.608437 4_4:-14236908 None:intergenic
TCAGGACTAGCAAGGTCGGG+AGG 0.608590 4_4:+14236977 Msa0671230:CDS
TGTACACCTGCGGAGAGGAG+TGG 0.610586 4_4:-14237242 None:intergenic
CTTAGATGAAGAGCAAGGTA+GGG 0.612955 4_4:-14235540 None:intergenic
TAGAAAGAGGTATCTTATGG+TGG 0.614327 4_4:+14234319 Msa0671230:CDS
TGGCTTTGTCATCAAGTGTG+TGG 0.615307 4_4:-14234950 None:intergenic
CCATTCTTAGATGAAGAGCA+AGG 0.618532 4_4:-14235545 None:intergenic
CTTGGAGTGACATGAAGGGG+AGG 0.621314 4_4:-14234985 None:intergenic
GTAACATCCATGGCAGTCAA+GGG 0.621567 4_4:-14235717 None:intergenic
CTTGATGACAAAGCCAGTGG+AGG 0.623445 4_4:+14234957 Msa0671230:CDS
GCTGAGAGAATGCAAAGCAA+AGG 0.624230 4_4:+14235516 Msa0671230:CDS
TTGATGACAAAGCCAGTGGA+GGG 0.626586 4_4:+14234958 Msa0671230:CDS
AAGAATGGAAAAGGATGTGT+GGG 0.627477 4_4:+14235560 Msa0671230:CDS
CAGGACTAGCAAGGTCGGGA+GGG 0.628531 4_4:+14236978 Msa0671230:CDS
TTGGAGTGACATGAAGGGGA+GGG 0.631381 4_4:-14234984 None:intergenic
AATGGTAGAGAGAGAAAGGG+CGG 0.639855 4_4:-14233400 None:intergenic
TTTGGTAAAATCTGAACTTG+GGG 0.642353 4_4:+14234004 Msa0671230:CDS
TTGCAAGCAAAGGTTCCTAG+TGG 0.655758 4_4:+14234158 Msa0671230:CDS
TAAGAATGGAAAAGGATGTG+TGG 0.664578 4_4:+14235559 Msa0671230:CDS
GCCGGAACTCAGGACTAGCA+AGG 0.665088 4_4:+14236969 Msa0671230:CDS
GGATTGTTAAGAATAGAAAG+AGG 0.665202 4_4:+14234306 Msa0671230:CDS
TCTCTACTGAAATCCAAACA+AGG 0.666937 4_4:-14234263 None:intergenic
ATAGTCATACCTGAAGCGCA+TGG 0.668438 4_4:-14236353 None:intergenic
TCAAACTCAGGACTTAGACC+GGG 0.669861 4_4:-14235600 None:intergenic
ACACTTGATGACAAAGCCAG+TGG 0.673399 4_4:+14234954 Msa0671230:CDS
GAATAATGCGTGTACACCTG+CGG 0.675961 4_4:-14237252 None:intergenic
CTGGTGGGATGAATCAGCAG+AGG 0.678713 4_4:+14234348 Msa0671230:CDS
CGGAAACGGATACACAAACG+TGG 0.684953 4_4:+14237102 Msa0671230:three_prime_UTR
TCTTGCCAATGATGTGCGCG+TGG 0.696204 4_4:+14234912 Msa0671230:CDS
ATGCGTGTACACCTGCGGAG+AGG 0.698097 4_4:-14237247 None:intergenic
TGTTAAACTCTGCTTCAGGG+AGG 0.699216 4_4:-14236909 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTTTATTATAGAAAA+TGG - chr4_4:14233462-14233481 None:intergenic 10.0%
!!! AATAAAAATGAGATTTTTAA+TGG + chr4_4:14233975-14233994 Msa0671230:CDS 10.0%
!!! TTTATTTGTTTTTGATTTAT+AGG + chr4_4:14236085-14236104 Msa0671230:intron 10.0%
!! ATATTTAGCTGTTATAAATT+TGG + chr4_4:14234642-14234661 Msa0671230:intron 15.0%
!!! TTTATTATGTTTTTCCTTAT+TGG + chr4_4:14234828-14234847 Msa0671230:intron 15.0%
!! TTATTAAATATGAAGACTAA+AGG - chr4_4:14236561-14236580 None:intergenic 15.0%
!!! ATGATACAATCAATTATTTT+AGG + chr4_4:14236614-14236633 Msa0671230:intron 15.0%
!!! TGATACAATCAATTATTTTA+GGG + chr4_4:14236615-14236634 Msa0671230:intron 15.0%
!! TTTGGTATAAAAATATTACA+CGG + chr4_4:14237082-14237101 Msa0671230:three_prime_UTR 15.0%
!! ATAGAAAATGGAATAATAAG+AGG - chr4_4:14233450-14233469 None:intergenic 20.0%
!!! ATTTTTGTTGAAGATTTCTT+TGG - chr4_4:14233738-14233757 None:intergenic 20.0%
!! ATATTGTGAAGATTTATGTA+AGG + chr4_4:14234501-14234520 Msa0671230:intron 20.0%
!! GCTAAATATATATCCAAATT+TGG - chr4_4:14234631-14234650 None:intergenic 20.0%
!!! AATATTTTTGTTCTTGATAC+TGG + chr4_4:14234734-14234753 Msa0671230:intron 20.0%
!! TTCTGTGGAAATAAAAATTT+GGG + chr4_4:14235200-14235219 Msa0671230:intron 20.0%
!! AGAAGAATTTAATATTTACC+TGG - chr4_4:14235742-14235761 None:intergenic 20.0%
!!! AAATTCTTCTACTTTTGTAT+AGG + chr4_4:14235752-14235771 Msa0671230:intron 20.0%
!! TTACACTTATGAAACTATTT+GGG + chr4_4:14235815-14235834 Msa0671230:intron 20.0%
!! AAAAAGATGCAAGAATTTAT+TGG + chr4_4:14236130-14236149 Msa0671230:CDS 20.0%
!! AAAAGATGCAAGAATTTATT+GGG + chr4_4:14236131-14236150 Msa0671230:CDS 20.0%
!!! TGTGCTTTTAGTAATTAAAA+AGG + chr4_4:14236587-14236606 Msa0671230:intron 20.0%
!!! TTATGGAGAAAATATGTTTT+TGG - chr4_4:14236668-14236687 None:intergenic 20.0%
!! AATTGCATGATTACAATTTA+TGG - chr4_4:14236685-14236704 None:intergenic 20.0%
!! ATAAAAATATTACACGGAAA+CGG + chr4_4:14237088-14237107 Msa0671230:three_prime_UTR 20.0%
!! CATACATATTAAGCATATTA+AGG - chr4_4:14237277-14237296 None:intergenic 20.0%
!! TTTATTCAGATATATGTACA+AGG - chr4_4:14237426-14237445 None:intergenic 20.0%
!!! AAACAATCATAAAATTTTGC+AGG - chr4_4:14237472-14237491 None:intergenic 20.0%
!!! AAAGAGAAAGAAAGAGTATT+GGG - chr4_4:14233596-14233615 None:intergenic 25.0%
! ATGGAATTGATTATTTGTGA+AGG - chr4_4:14233675-14233694 None:intergenic 25.0%
! TTGAATAGAAGAAGATGAAA+TGG - chr4_4:14233694-14233713 None:intergenic 25.0%
! AAAAGAAGAACAATCTTGAA+GGG - chr4_4:14233803-14233822 None:intergenic 25.0%
! AAAAAGAAGAACAATCTTGA+AGG - chr4_4:14233804-14233823 None:intergenic 25.0%
!!! TTTTTCACACTCTTATTTCT+TGG + chr4_4:14233819-14233838 Msa0671230:CDS 25.0%
!!! GTTTTTGCTTTGTTTATGAT+TGG + chr4_4:14233843-14233862 Msa0671230:CDS 25.0%
!!! GATTTTTAATGGTGATGTTT+TGG + chr4_4:14233986-14234005 Msa0671230:CDS 25.0%
!! TTTTGGTAAAATCTGAACTT+GGG + chr4_4:14234003-14234022 Msa0671230:CDS 25.0%
! TTTGATTCAAAATCTGAACT+TGG + chr4_4:14234056-14234075 Msa0671230:CDS 25.0%
! AACATTACATTAGCAATCAA+AGG - chr4_4:14234140-14234159 None:intergenic 25.0%
!!! ATGACATTAACTTGCAAAAT+AGG - chr4_4:14234431-14234450 None:intergenic 25.0%
!! TTTTGCAAGTTAATGTCATT+TGG + chr4_4:14234432-14234451 Msa0671230:CDS 25.0%
!! TTTGCAAGTTAATGTCATTT+GGG + chr4_4:14234433-14234452 Msa0671230:CDS 25.0%
! TATACTATTCTTCTTGTTCA+TGG + chr4_4:14234472-14234491 Msa0671230:intron 25.0%
! CTTAGATTATACTCCAATAT+TGG - chr4_4:14234565-14234584 None:intergenic 25.0%
! ATCTTCATAAAGAACAACTT+TGG + chr4_4:14234670-14234689 Msa0671230:intron 25.0%
!!! ATGTTTTTCCTTATTGGTTT+TGG + chr4_4:14234834-14234853 Msa0671230:intron 25.0%
! AAACAAAACCAAAACCAATA+AGG - chr4_4:14234845-14234864 None:intergenic 25.0%
!!! TCTTATGTTTTAGCATCTTT+AGG + chr4_4:14235061-14235080 Msa0671230:intron 25.0%
! CTTCTGTGGAAATAAAAATT+TGG + chr4_4:14235199-14235218 Msa0671230:intron 25.0%
! CTTACACTTATGAAACTATT+TGG + chr4_4:14235814-14235833 Msa0671230:intron 25.0%
! GTTTCCACATCTTTATAATA+AGG + chr4_4:14236206-14236225 Msa0671230:CDS 25.0%
! TCTTCCTTATTATAAAGATG+TGG - chr4_4:14236213-14236232 None:intergenic 25.0%
! GTAATCATGCTTACATATAT+TGG + chr4_4:14236447-14236466 Msa0671230:intron 25.0%
! ATACAGTATATATGAATGCA+TGG - chr4_4:14236473-14236492 None:intergenic 25.0%
!! AGGTGAGTACAAATTTTATA+GGG - chr4_4:14236541-14236560 None:intergenic 25.0%
!! AAGGTGAGTACAAATTTTAT+AGG - chr4_4:14236542-14236561 None:intergenic 25.0%
!!! AATTATTTTAGGGATGTTGA+TGG + chr4_4:14236625-14236644 Msa0671230:intron 25.0%
!!! ATTATTTTAGGGATGTTGAT+GGG + chr4_4:14236626-14236645 Msa0671230:intron 25.0%
! AATTAAGATAGTCACATTGA+TGG + chr4_4:14236701-14236720 Msa0671230:intron 25.0%
! GCTTAATATGTATGCAATTT+AGG + chr4_4:14237283-14237302 Msa0671230:three_prime_UTR 25.0%
! TCTTTCTATAGATCTTGTAT+AGG + chr4_4:14237359-14237378 Msa0671230:three_prime_UTR 25.0%
!! TAGTTTGTAGATAATGAAGA+TGG - chr4_4:14237501-14237520 None:intergenic 25.0%
! CTACTTTACATATACAGATA+TGG + chr4_4:14237518-14237537 Msa0671230:three_prime_UTR 25.0%
! GGTTTTTCTTGTTGAATGTT+TGG - chr4_4:14233575-14233594 None:intergenic 30.0%
! GAAAGAGAAAGAAAGAGTAT+TGG - chr4_4:14233597-14233616 None:intergenic 30.0%
TCTTTCTCTTTCATCTTCAA+TGG + chr4_4:14233605-14233624 Msa0671230:exon 30.0%
! GTTTTTCCATTTTGTTAAGG+TGG - chr4_4:14233781-14233800 None:intergenic 30.0%
! AGGGTTTTTCCATTTTGTTA+AGG - chr4_4:14233784-14233803 None:intergenic 30.0%
TTTCAACATTTGTAGCACAT+GGG - chr4_4:14233899-14233918 None:intergenic 30.0%
! TTTTCAACATTTGTAGCACA+TGG - chr4_4:14233900-14233919 None:intergenic 30.0%
AAAAGCCCTTAATCTCAAAT+GGG + chr4_4:14233916-14233935 Msa0671230:CDS 30.0%
TGGTTCAAAATCTGAACTTA+AGG + chr4_4:14233941-14233960 Msa0671230:CDS 30.0%
!! GTTTTGGTAAAATCTGAACT+TGG + chr4_4:14234002-14234021 Msa0671230:CDS 30.0%
TTTGGTAAAATCTGAACTTG+GGG + chr4_4:14234004-14234023 Msa0671230:CDS 30.0%
AAAACCCTTCAATCTCAAAT+GGG + chr4_4:14234033-14234052 Msa0671230:CDS 30.0%
TAATGTAATGTTGCAAGCAA+AGG + chr4_4:14234148-14234167 Msa0671230:CDS 30.0%
ATTACAGAAGAGAAAGTGAT+GGG + chr4_4:14234285-14234304 Msa0671230:CDS 30.0%
GGATTGTTAAGAATAGAAAG+AGG + chr4_4:14234306-14234325 Msa0671230:CDS 30.0%
!! GAATAGAAAGAGGTATCTTA+TGG + chr4_4:14234316-14234335 Msa0671230:CDS 30.0%
! TTGCAAGTTAATGTCATTTG+GGG + chr4_4:14234434-14234453 Msa0671230:CDS 30.0%
AATCTTCCTAAGACCAATAT+TGG + chr4_4:14234549-14234568 Msa0671230:intron 30.0%
!! TATACTCCAATATTGGTCTT+AGG - chr4_4:14234558-14234577 None:intergenic 30.0%
TCTTCTAGCATTCACAAATA+AGG - chr4_4:14234795-14234814 None:intergenic 30.0%
GCAAAAGTAGACAAAAACTT+AGG - chr4_4:14235094-14235113 None:intergenic 30.0%
TTGCGTTGAAAAATTTCACA+TGG + chr4_4:14235110-14235129 Msa0671230:intron 30.0%
! AATAAAAGCTTTGACTTCTG+TGG + chr4_4:14235185-14235204 Msa0671230:intron 30.0%
TGTTGAACTAGAGTTTAACA+TGG + chr4_4:14235243-14235262 Msa0671230:intron 30.0%
ATCTTCATCTGTGTAACAAT+CGG + chr4_4:14235396-14235415 Msa0671230:intron 30.0%
TAAAAGCAACCTGAAAACAT+AGG - chr4_4:14235449-14235468 None:intergenic 30.0%
TCTTCATCTAAGAATGGAAA+AGG + chr4_4:14235551-14235570 Msa0671230:CDS 30.0%
TTTACAATCTCATCAAACTC+AGG - chr4_4:14235615-14235634 None:intergenic 30.0%
ATGAGATTGTAAAGAATGAG+AGG + chr4_4:14235622-14235641 Msa0671230:CDS 30.0%
! CTCTTATTCGAGTTTATTAC+TGG + chr4_4:14235789-14235808 Msa0671230:intron 30.0%
! ACTGATTTCATAAGCTATCT+TGG - chr4_4:14235904-14235923 None:intergenic 30.0%
TGTTTATCCAAAGATCCTAT+AGG + chr4_4:14236017-14236036 Msa0671230:intron 30.0%
TATAGGTTGCTTAAAGCTTT+AGG + chr4_4:14236102-14236121 Msa0671230:intron 30.0%
TATAAAGATGTGGAAACTCA+TGG - chr4_4:14236203-14236222 None:intergenic 30.0%
CTCAGAAACTATGTAATCTA+TGG - chr4_4:14236292-14236311 None:intergenic 30.0%
TTATAGGGACTAAAGTTGAA+AGG - chr4_4:14236526-14236545 None:intergenic 30.0%
AGGGAAGAATTCAGAAAATA+GGG - chr4_4:14236892-14236911 None:intergenic 30.0%
GAATTGCATCAGAATTCATT+AGG + chr4_4:14236943-14236962 Msa0671230:CDS 30.0%
! AGAATCAATGTCAAAGCTTT+TGG + chr4_4:14237064-14237083 Msa0671230:three_prime_UTR 30.0%
!!! AATGTTGCTTTTGAGAACTA+TGG - chr4_4:14237202-14237221 None:intergenic 30.0%
TGTATGTAACATCCCTTTAA+TGG - chr4_4:14237320-14237339 None:intergenic 30.0%
AAGAATGGTAGAGAGAGAAA+GGG - chr4_4:14233406-14233425 None:intergenic 35.0%
!! CAACAAAGCTTTGTTGTGTT+GGG - chr4_4:14233511-14233530 None:intergenic 35.0%
!! ACAACAAAGCTTTGTTGTGT+TGG - chr4_4:14233512-14233531 None:intergenic 35.0%
TCTTCACCACCTTAACAAAA+TGG + chr4_4:14233772-14233791 Msa0671230:CDS 35.0%
!!! GCACATGGGGTTTTAAAAAA+CGG - chr4_4:14233885-14233904 None:intergenic 35.0%
TTCAACATTTGTAGCACATG+GGG - chr4_4:14233898-14233917 None:intergenic 35.0%
GAAAAGCCCTTAATCTCAAA+TGG + chr4_4:14233915-14233934 Msa0671230:CDS 35.0%
! CCAAACCCATTTGAGATTAA+GGG - chr4_4:14233924-14233943 None:intergenic 35.0%
! ACCAAACCCATTTGAGATTA+AGG - chr4_4:14233925-14233944 None:intergenic 35.0%
CAAAACCCTTCAATCTCAAA+TGG + chr4_4:14234032-14234051 Msa0671230:CDS 35.0%
! CAAACCCATTTGAGATTGAA+GGG - chr4_4:14234040-14234059 None:intergenic 35.0%
! TCAAACCCATTTGAGATTGA+AGG - chr4_4:14234041-14234060 None:intergenic 35.0%
TGACTTGAAGGATGAAGAAA+AGG + chr4_4:14234184-14234203 Msa0671230:CDS 35.0%
GACTTGAAGGATGAAGAAAA+GGG + chr4_4:14234185-14234204 Msa0671230:CDS 35.0%
TCTCTACTGAAATCCAAACA+AGG - chr4_4:14234266-14234285 None:intergenic 35.0%
GATTACAGAAGAGAAAGTGA+TGG + chr4_4:14234284-14234303 Msa0671230:CDS 35.0%
!! TAGAAAGAGGTATCTTATGG+TGG + chr4_4:14234319-14234338 Msa0671230:CDS 35.0%
! TATCTTATGGTGGTTGTTTC+TGG + chr4_4:14234329-14234348 Msa0671230:CDS 35.0%
! TTGATGCTGTTGTTATTGCT+AGG + chr4_4:14234381-14234400 Msa0671230:CDS 35.0%
! GTTGTTATTGCTAGGATTCT+TGG + chr4_4:14234389-14234408 Msa0671230:CDS 35.0%
AACAAGATCAAACTATGCTG+AGG - chr4_4:14234588-14234607 None:intergenic 35.0%
TGCTTGATGAAGACCAAATT+TGG + chr4_4:14234615-14234634 Msa0671230:intron 35.0%
TTGTTTCCAAGTTCTTGAAC+TGG - chr4_4:14235492-14235511 None:intergenic 35.0%
TAAGAATGGAAAAGGATGTG+TGG + chr4_4:14235559-14235578 Msa0671230:CDS 35.0%
AAGAATGGAAAAGGATGTGT+GGG + chr4_4:14235560-14235579 Msa0671230:CDS 35.0%
GACTTACCATGAAAGAAAGA+TGG + chr4_4:14235683-14235702 Msa0671230:CDS 35.0%
TATTTACCTGGTAACATCCA+TGG - chr4_4:14235730-14235749 None:intergenic 35.0%
! CTTTTGTATAGGAGTCTGTT+TGG + chr4_4:14235763-14235782 Msa0671230:intron 35.0%
AAACTATTTGGGAGAGCTTA+TGG + chr4_4:14235826-14235845 Msa0671230:intron 35.0%
! TGTGGAAACTCATGGATTAA+TGG - chr4_4:14236195-14236214 None:intergenic 35.0%
AGAGAAGCTTTCTTCGAAAA+TGG - chr4_4:14236261-14236280 None:intergenic 35.0%
TTCGAAGAAAGCTTCTCTAA+TGG + chr4_4:14236263-14236282 Msa0671230:CDS 35.0%
!! TTGATGGGTCGTGATTTTAT+AGG + chr4_4:14236641-14236660 Msa0671230:intron 35.0%
ACCTAAATGTTATTCCTTCC+AGG + chr4_4:14236807-14236826 Msa0671230:intron 35.0%
CCTAAATGTTATTCCTTCCA+GGG + chr4_4:14236808-14236827 Msa0671230:intron 35.0%
! GGATGCATTTGTCTTTTGTT+TGG - chr4_4:14236871-14236890 None:intergenic 35.0%
GAGGGAAGAATTCAGAAAAT+AGG - chr4_4:14236893-14236912 None:intergenic 35.0%
GTTTCCTGTGATCCATTAAA+GGG + chr4_4:14237305-14237324 Msa0671230:three_prime_UTR 35.0%
GAAGAATGGTAGAGAGAGAA+AGG - chr4_4:14233407-14233426 None:intergenic 40.0%
AAAGAGAGAGGAAGGAAGAA+TGG - chr4_4:14233421-14233440 None:intergenic 40.0%
! CCCTTAATCTCAAATGGGTT+TGG + chr4_4:14233921-14233940 Msa0671230:CDS 40.0%
AAGGATGAAGAAAAGGGTGA+AGG + chr4_4:14234191-14234210 Msa0671230:CDS 40.0%
AAAGGGTGAAGGAGTTGAAA+AGG + chr4_4:14234202-14234221 Msa0671230:CDS 40.0%
AAGGGTGAAGGAGTTGAAAA+GGG + chr4_4:14234203-14234222 Msa0671230:CDS 40.0%
! GAGTTGAAAAGGGTGAGTTT+TGG + chr4_4:14234213-14234232 Msa0671230:CDS 40.0%
!!! AGTTTTGGAAGCAACCAGAT+GGG + chr4_4:14234228-14234247 Msa0671230:CDS 40.0%
!! GTTAGGGTATAAGCCTTGTT+TGG + chr4_4:14234250-14234269 Msa0671230:CDS 40.0%
!! TTATGGTGGTTGTTTCTGGT+GGG + chr4_4:14234333-14234352 Msa0671230:CDS 40.0%
! ATGTCATTTGGGGAGATGAA+AGG + chr4_4:14234444-14234463 Msa0671230:CDS 40.0%
TTTGTCATCAAGTGTGTGGA+TGG - chr4_4:14234949-14234968 None:intergenic 40.0%
!! TGTTTTGCTTTTACGCAGCT+TGG + chr4_4:14235299-14235318 Msa0671230:CDS 40.0%
! TTTTACGCAGCTTGGATTCA+AGG + chr4_4:14235307-14235326 Msa0671230:CDS 40.0%
CTTGGATTCAAGGCTATCAA+AGG + chr4_4:14235317-14235336 Msa0671230:CDS 40.0%
CTTAGATGAAGAGCAAGGTA+GGG - chr4_4:14235543-14235562 None:intergenic 40.0%
TCTTAGATGAAGAGCAAGGT+AGG - chr4_4:14235544-14235563 None:intergenic 40.0%
CCATTCTTAGATGAAGAGCA+AGG - chr4_4:14235548-14235567 None:intergenic 40.0%
CCTTGCTCTTCATCTAAGAA+TGG + chr4_4:14235545-14235564 Msa0671230:CDS 40.0%
ATCAAACTCAGGACTTAGAC+CGG - chr4_4:14235604-14235623 None:intergenic 40.0%
TACCATGAAAGAAAGATGGC+TGG + chr4_4:14235687-14235706 Msa0671230:CDS 40.0%
AGTGAAGCCTATAGGATCTT+TGG - chr4_4:14236027-14236046 None:intergenic 40.0%
CACTGAAAAGTGAAGCCTAT+AGG - chr4_4:14236035-14236054 None:intergenic 40.0%
!! CTATAGGCTTCACTTTTCAG+TGG + chr4_4:14236033-14236052 Msa0671230:intron 40.0%
GATGCAAGAATTTATTGGGC+CGG + chr4_4:14236135-14236154 Msa0671230:CDS 40.0%
ATGCAAGAATTTATTGGGCC+GGG + chr4_4:14236136-14236155 Msa0671230:CDS 40.0%
!! GAAAATGGTTCTAGTTCTCC+AGG - chr4_4:14236246-14236265 None:intergenic 40.0%
! GATGTTTTCATGCCATCTCA+TGG + chr4_4:14236318-14236337 Msa0671230:CDS 40.0%
CCATCTCATGGAGGAAATAT+GGG + chr4_4:14236330-14236349 Msa0671230:CDS 40.0%
CCCTGGAAGGAATAACATTT+AGG - chr4_4:14236811-14236830 None:intergenic 40.0%
GTATTTCTTGTGTCCTGCAT+AGG - chr4_4:14236842-14236861 None:intergenic 40.0%
TGCTGTTAAACTCTGCTTCA+GGG - chr4_4:14236915-14236934 None:intergenic 40.0%
ATGCTGTTAAACTCTGCTTC+AGG - chr4_4:14236916-14236935 None:intergenic 40.0%
TCAGAATTCATTAGGACAGC+CGG + chr4_4:14236951-14236970 Msa0671230:CDS 40.0%
! GTGGAAGCAAGTAGTACTTT+TGG + chr4_4:14237121-14237140 Msa0671230:three_prime_UTR 40.0%
TTTATCCCTCGAAAACTGCT+TGG + chr4_4:14237159-14237178 Msa0671230:three_prime_UTR 40.0%
GGTTTCCTGTGATCCATTAA+AGG + chr4_4:14237304-14237323 Msa0671230:three_prime_UTR 40.0%
! ACATCCCTTTAATGGATCAC+AGG - chr4_4:14237312-14237331 None:intergenic 40.0%
GTAGATAATGAAGATGGAGC+TGG - chr4_4:14237495-14237514 None:intergenic 40.0%
AATGGTAGAGAGAGAAAGGG+CGG - chr4_4:14233403-14233422 None:intergenic 45.0%
AAGAGGAGAGAGAAAGAGAG+AGG - chr4_4:14233433-14233452 None:intergenic 45.0%
TTGCAAGCAAAGGTTCCTAG+TGG + chr4_4:14234158-14234177 Msa0671230:CDS 45.0%
!! TCCTAGTGGAGTTGACTTGA+AGG + chr4_4:14234172-14234191 Msa0671230:CDS 45.0%
TCCTTCAAGTCAACTCCACT+AGG - chr4_4:14234176-14234195 None:intergenic 45.0%
!!! GAGTTTTGGAAGCAACCAGA+TGG + chr4_4:14234227-14234246 Msa0671230:CDS 45.0%
!! CTTATGGTGGTTGTTTCTGG+TGG + chr4_4:14234332-14234351 Msa0671230:CDS 45.0%
!! TAGGATTCTTGGTGCTGCTT+TGG + chr4_4:14234400-14234419 Msa0671230:CDS 45.0%
!! TGGCTTTGTCATCAAGTGTG+TGG - chr4_4:14234953-14234972 None:intergenic 45.0%
! ACACTTGATGACAAAGCCAG+TGG + chr4_4:14234954-14234973 Msa0671230:CDS 45.0%
! TTGATGACAAAGCCAGTGGA+GGG + chr4_4:14234958-14234977 Msa0671230:CDS 45.0%
CAACTTGGAGTGACATGAAG+GGG - chr4_4:14234991-14235010 None:intergenic 45.0%
CCAACTTGGAGTGACATGAA+GGG - chr4_4:14234992-14235011 None:intergenic 45.0%
TCCAACTTGGAGTGACATGA+AGG - chr4_4:14234993-14235012 None:intergenic 45.0%
!!! CTCTTTTGAGATAGCGCGAA+CGG - chr4_4:14235017-14235036 None:intergenic 45.0%
CCTTCTCTGTTGAACTGCAA+AGG - chr4_4:14235279-14235298 None:intergenic 45.0%
CCTTTGCAGTTCAACAGAGA+AGG + chr4_4:14235276-14235295 Msa0671230:intron 45.0%
! CGAAGCTTCTGAAGATCTGA+AGG - chr4_4:14235348-14235367 None:intergenic 45.0%
TCTTCAGAAGCTTCGATGCA+AGG + chr4_4:14235353-14235372 Msa0671230:CDS 45.0%
! CTCTGCTCACCTATGTTTTC+AGG + chr4_4:14235437-14235456 Msa0671230:intron 45.0%
GCTGAGAGAATGCAAAGCAA+AGG + chr4_4:14235516-14235535 Msa0671230:CDS 45.0%
TCAAACTCAGGACTTAGACC+GGG - chr4_4:14235603-14235622 None:intergenic 45.0%
GATCTTCCTTTCAAGAGCTC+CGG - chr4_4:14235657-14235676 None:intergenic 45.0%
GACCAGCCATCTTTCTTTCA+TGG - chr4_4:14235692-14235711 None:intergenic 45.0%
GTAACATCCATGGCAGTCAA+GGG - chr4_4:14235720-14235739 None:intergenic 45.0%
AGCTATCTTGGAGAGCTTGT+GGG - chr4_4:14235892-14235911 None:intergenic 45.0%
AAGCTATCTTGGAGAGCTTG+TGG - chr4_4:14235893-14235912 None:intergenic 45.0%
TGCAAGAATTTATTGGGCCG+GGG + chr4_4:14236137-14236156 Msa0671230:CDS 45.0%
AAAGCTTCTTTCCCACCCAA+TGG - chr4_4:14236168-14236187 None:intergenic 45.0%
AAGGAAGATCTTGCATTGCC+TGG + chr4_4:14236225-14236244 Msa0671230:CDS 45.0%
! GTTTTCATGCCATCTCATGG+AGG + chr4_4:14236321-14236340 Msa0671230:CDS 45.0%
GCCATCTCATGGAGGAAATA+TGG + chr4_4:14236329-14236348 Msa0671230:CDS 45.0%
CCCATATTTCCTCCATGAGA+TGG - chr4_4:14236333-14236352 None:intergenic 45.0%
ATAGTCATACCTGAAGCGCA+TGG - chr4_4:14236356-14236375 None:intergenic 45.0%
TTATTCCTTCCAGGGTCACA+GGG + chr4_4:14236816-14236835 Msa0671230:intron 45.0%
GTTAAACTCTGCTTCAGGGA+GGG - chr4_4:14236911-14236930 None:intergenic 45.0%
TGTTAAACTCTGCTTCAGGG+AGG - chr4_4:14236912-14236931 None:intergenic 45.0%
GAATAATGCGTGTACACCTG+CGG - chr4_4:14237255-14237274 None:intergenic 45.0%
GGAGAGAGAAAGAGAGAGGA+AGG - chr4_4:14233429-14233448 None:intergenic 50.0%
! TGGAAGCAACCAGATGGGTT+AGG + chr4_4:14234233-14234252 Msa0671230:CDS 50.0%
!! GGAAGCAACCAGATGGGTTA+GGG + chr4_4:14234234-14234253 Msa0671230:CDS 50.0%
GGCTTATACCCTAACCCATC+TGG - chr4_4:14234245-14234264 None:intergenic 50.0%
TGAAACCACGCGCACATCAT+TGG - chr4_4:14234920-14234939 None:intergenic 50.0%
! CTTGATGACAAAGCCAGTGG+AGG + chr4_4:14234957-14234976 Msa0671230:CDS 50.0%
TTGGAGTGACATGAAGGGGA+GGG - chr4_4:14234987-14235006 None:intergenic 50.0%
CCCTTCATGTCACTCCAAGT+TGG + chr4_4:14234989-14235008 Msa0671230:CDS 50.0%
GCGAAGCCAGTTCAAGAACT+TGG + chr4_4:14235483-14235502 Msa0671230:CDS 50.0%
TGGAAAAGGATGTGTGGGTG+AGG + chr4_4:14235565-14235584 Msa0671230:CDS 50.0%
GAATGAGAGGAGAGAACGAC+CGG + chr4_4:14235635-14235654 Msa0671230:CDS 50.0%
TCTTTGTCCCTTGACTGCCA+TGG + chr4_4:14235710-14235729 Msa0671230:CDS 50.0%
GGTAACATCCATGGCAGTCA+AGG - chr4_4:14235721-14235740 None:intergenic 50.0%
TGACTGCCATGGATGTTACC+AGG + chr4_4:14235721-14235740 Msa0671230:CDS 50.0%
GCAAGAATTTATTGGGCCGG+GGG + chr4_4:14236138-14236157 Msa0671230:CDS 50.0%
AAATATGGGCCATGCGCTTC+AGG + chr4_4:14236344-14236363 Msa0671230:CDS 50.0%
GTTATTCCTTCCAGGGTCAC+AGG + chr4_4:14236815-14236834 Msa0671230:intron 50.0%
ACCTTGCTAGTCCTGAGTTC+CGG - chr4_4:14236973-14236992 None:intergenic 50.0%
! GAACTCAGGACTAGCAAGGT+CGG + chr4_4:14236973-14236992 Msa0671230:CDS 50.0%
! AACTCAGGACTAGCAAGGTC+GGG + chr4_4:14236974-14236993 Msa0671230:CDS 50.0%
AGTCGTTGCACATGCACTCT+GGG - chr4_4:14237023-14237042 None:intergenic 50.0%
CGGAAACGGATACACAAACG+TGG + chr4_4:14237102-14237121 Msa0671230:three_prime_UTR 50.0%
ATCCCTCGAAAACTGCTTGG+AGG + chr4_4:14237162-14237181 Msa0671230:three_prime_UTR 50.0%
! AGCCTCCAAGCAGTTTTCGA+GGG - chr4_4:14237167-14237186 None:intergenic 50.0%
! TAGCCTCCAAGCAGTTTTCG+AGG - chr4_4:14237168-14237187 None:intergenic 50.0%
! ACTGCTTGGAGGCTACTTTC+AGG + chr4_4:14237173-14237192 Msa0671230:three_prime_UTR 50.0%
CTGGTGGGATGAATCAGCAG+AGG + chr4_4:14234348-14234367 Msa0671230:CDS 55.0%
TCTTGCCAATGATGTGCGCG+TGG + chr4_4:14234912-14234931 Msa0671230:CDS 55.0%
CTTGGAGTGACATGAAGGGG+AGG - chr4_4:14234988-14235007 None:intergenic 55.0%
TAGCGCGAACGGATCCAACT+TGG - chr4_4:14235006-14235025 None:intergenic 55.0%
AAGGATGTGTGGGTGAGGAC+TGG + chr4_4:14235570-14235589 Msa0671230:CDS 55.0%
!! GTGAGGACTGGTTGTCTACC+CGG + chr4_4:14235582-14235601 Msa0671230:CDS 55.0%
GAACGACCGGAGCTCTTGAA+AGG + chr4_4:14235648-14235667 Msa0671230:CDS 55.0%
CATTAGGACAGCCGGAACTC+AGG + chr4_4:14236959-14236978 Msa0671230:CDS 55.0%
!! TTGCACATGCACTCTGGGAC+AGG - chr4_4:14237018-14237037 None:intergenic 55.0%
GAGTCGTTGCACATGCACTC+TGG - chr4_4:14237024-14237043 None:intergenic 55.0%
GTAGAGAGAGAAAGGGCGGC+GGG - chr4_4:14233399-14233418 None:intergenic 60.0%
GGTAGAGAGAGAAAGGGCGG+CGG - chr4_4:14233400-14233419 None:intergenic 60.0%
ATAGGCCCTGTGACCCTGGA+AGG - chr4_4:14236824-14236843 None:intergenic 60.0%
! GCCGGAACTCAGGACTAGCA+AGG + chr4_4:14236969-14236988 Msa0671230:CDS 60.0%
! TCAGGACTAGCAAGGTCGGG+AGG + chr4_4:14236977-14236996 Msa0671230:CDS 60.0%
! CAGGACTAGCAAGGTCGGGA+GGG + chr4_4:14236978-14236997 Msa0671230:CDS 60.0%
TGTACACCTGCGGAGAGGAG+TGG - chr4_4:14237245-14237264 None:intergenic 60.0%
ATGCGTGTACACCTGCGGAG+AGG - chr4_4:14237250-14237269 None:intergenic 60.0%
TTGGGCCGGGGGACAACCAT+TGG + chr4_4:14236149-14236168 Msa0671230:CDS 65.0%
TGGGCCGGGGGACAACCATT+GGG + chr4_4:14236150-14236169 Msa0671230:CDS 65.0%
CCCACCCAATGGTTGTCCCC+CGG - chr4_4:14236157-14236176 None:intergenic 65.0%
CCGGGGGACAACCATTGGGT+GGG + chr4_4:14236154-14236173 Msa0671230:CDS 65.0%
CTGCATAGGCCCTGTGACCC+TGG - chr4_4:14236828-14236847 None:intergenic 65.0%
CAGGGTCACAGGGCCTATGC+AGG + chr4_4:14236826-14236845 Msa0671230:intron 65.0%
TGTCAGCCACTCCTCTCCGC+AGG + chr4_4:14237236-14237255 Msa0671230:three_prime_UTR 65.0%
AGGGGAGGGCTTCCCTCCAC+TGG - chr4_4:14234973-14234992 None:intergenic 70.0%
GCCGGGGGACAACCATTGGG+TGG + chr4_4:14236153-14236172 Msa0671230:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 14233386 14237569 14233386 ID=Msa0671230;Name=Msa0671230
chr4_4 mRNA 14233386 14237569 14233386 ID=Msa0671230-mRNA-1;Parent=Msa0671230;Name=Msa0671230-mRNA-1;_AED=0.08;_eAED=0.08;_QI=238|1|1|1|1|1|6|510|623
chr4_4 exon 14233386 14234465 14233386 ID=Msa0671230-mRNA-1:exon:4365;Parent=Msa0671230-mRNA-1
chr4_4 exon 14234870 14235035 14234870 ID=Msa0671230-mRNA-1:exon:4366;Parent=Msa0671230-mRNA-1
chr4_4 exon 14235285 14235374 14235285 ID=Msa0671230-mRNA-1:exon:4367;Parent=Msa0671230-mRNA-1
chr4_4 exon 14235459 14235742 14235459 ID=Msa0671230-mRNA-1:exon:4368;Parent=Msa0671230-mRNA-1
chr4_4 exon 14236107 14236365 14236107 ID=Msa0671230-mRNA-1:exon:4369;Parent=Msa0671230-mRNA-1
chr4_4 exon 14236829 14237569 14236829 ID=Msa0671230-mRNA-1:exon:4370;Parent=Msa0671230-mRNA-1
chr4_4 five_prime_UTR 14233386 14233623 14233386 ID=Msa0671230-mRNA-1:five_prime_utr;Parent=Msa0671230-mRNA-1
chr4_4 CDS 14233624 14234465 14233624 ID=Msa0671230-mRNA-1:cds;Parent=Msa0671230-mRNA-1
chr4_4 CDS 14234870 14235035 14234870 ID=Msa0671230-mRNA-1:cds;Parent=Msa0671230-mRNA-1
chr4_4 CDS 14235285 14235374 14235285 ID=Msa0671230-mRNA-1:cds;Parent=Msa0671230-mRNA-1
chr4_4 CDS 14235459 14235742 14235459 ID=Msa0671230-mRNA-1:cds;Parent=Msa0671230-mRNA-1
chr4_4 CDS 14236107 14236365 14236107 ID=Msa0671230-mRNA-1:cds;Parent=Msa0671230-mRNA-1
chr4_4 CDS 14236829 14237059 14236829 ID=Msa0671230-mRNA-1:cds;Parent=Msa0671230-mRNA-1
chr4_4 three_prime_UTR 14237060 14237569 14237060 ID=Msa0671230-mRNA-1:three_prime_utr;Parent=Msa0671230-mRNA-1
Gene Sequence

>Msa0671230

ATGGCTAAGTCAAAGAACAATGCAAAGAAACTCTCATACATCTCAGTTCCTTCACAAATAATCAATTCCATTTCATCTTCTTCTATTCAATCATTACTTGATGATCAAACTCCAAAGAAATCTTCAACAAAAATTGCTTCAAACTTCTTCTTCACCACCTTAACAAAATGGAAAAACCCTTCAAGATTGTTCTTCTTTTTCACACTCTTATTTCTTGGTGTTTTTGCTTTGTTTATGATTGGTTTCAATCTTAAAACACCGTTTTTTAAAACCCCATGTGCTACAAATGTTGAAAAGCCCTTAATCTCAAATGGGTTTGGTTCAAAATCTGAACTTAAGGTTGTTTCAATGAATAAAAATGAGATTTTTAATGGTGATGTTTTGGTAAAATCTGAACTTGGGGTTGTTCAAAACCCTTCAATCTCAAATGGGTTTGATTCAAAATCTGAACTTGGTGTTGTTTCAATGAATAAAGATGAGATTTTGAATGATGTTAGTTTGTCAAATTCACTTCCTTTGATTGCTAATGTAATGTTGCAAGCAAAGGTTCCTAGTGGAGTTGACTTGAAGGATGAAGAAAAGGGTGAAGGAGTTGAAAAGGGTGAGTTTTGGAAGCAACCAGATGGGTTAGGGTATAAGCCTTGTTTGGATTTCAGTAGAGATTACAGAAGAGAAAGTGATGGGATTGTTAAGAATAGAAAGAGGTATCTTATGGTGGTTGTTTCTGGTGGGATGAATCAGCAGAGGAATCAAATTGTTGATGCTGTTGTTATTGCTAGGATTCTTGGTGCTGCTTTGGTTGTTCCTATTTTGCAAGTTAATGTCATTTGGGGAGATGAAAGTGAATTTGCTGATATATTTGATTTTGAGCACTTCAAGAATGTTCTTGCCAATGATGTGCGCGTGGTTTCATCATTGCCATCCACACACTTGATGACAAAGCCAGTGGAGGGAAGCCCTCCCCTTCATGTCACTCCAAGTTGGATCCGTTCGCGCTATCTCAAAAGATTCAACAGAGAAGGTGTTTTGCTTTTACGCAGCTTGGATTCAAGGCTATCAAAGGATCTTCCTTCAGATCTTCAGAAGCTTCGATGCAAGGTTGCTTTTAGTGCATTGAGATTTGCGAAGCCAGTTCAAGAACTTGGAAACAAGATTGCTGAGAGAATGCAAAGCAAAGGACCCTACCTTGCTCTTCATCTAAGAATGGAAAAGGATGTGTGGGTGAGGACTGGTTGTCTACCCGGTCTAAGTCCTGAGTTTGATGAGATTGTAAAGAATGAGAGGAGAGAACGACCGGAGCTCTTGAAAGGAAGATCAAACATGACTTACCATGAAAGAAAGATGGCTGGTCTTTGTCCCTTGACTGCCATGGATGTTACCAGGTTGCTTAAAGCTTTAGGAGCTCAAAAAGATGCAAGAATTTATTGGGCCGGGGGACAACCATTGGGTGGGAAAGAAGCTTTGCATCCATTAATCCATGAGTTTCCACATCTTTATAATAAGGAAGATCTTGCATTGCCTGGAGAACTAGAACCATTTTCGAAGAAAGCTTCTCTAATGGCTGCCATAGATTACATAGTTTCTGAGAAGAGTGATGTTTTCATGCCATCTCATGGAGGAAATATGGGCCATGCGCTTCAGGGTCACAGGGCCTATGCAGGACACAAGAAATACATAACACCAAACAAAAGACAAATGCATCCCTATTTTCTGAATTCTTCCCTCCCTGAAGCAGAGTTTAACAGCATTGTCAAAGAATTGCATCAGAATTCATTAGGACAGCCGGAACTCAGGACTAGCAAGGTCGGGAGGGATGTCACGAAGTATCCTGTCCCAGAGTGCATGTGCAACGACTCACATAATGATTCGTAG
Protein sequence

>Msa0671230

MAKSKNNAKKLSYISVPSQIINSISSSSIQSLLDDQTPKKSSTKIASNFFFTTLTKWKNPSRLFFFFTLLFLGVFALFMIGFNLKTPFFKTPCATNVEKPLISNGFGSKSELKVVSMNKNEIFNGDVLVKSELGVVQNPSISNGFDSKSELGVVSMNKDEILNDVSLSNSLPLIANVMLQAKVPSGVDLKDEEKGEGVEKGEFWKQPDGLGYKPCLDFSRDYRRESDGIVKNRKRYLMVVVSGGMNQQRNQIVDAVVIARILGAALVVPILQVNVIWGDESEFADIFDFEHFKNVLANDVRVVSSLPSTHLMTKPVEGSPPLHVTPSWIRSRYLKRFNREGVLLLRSLDSRLSKDLPSDLQKLRCKVAFSALRFAKPVQELGNKIAERMQSKGPYLALHLRMEKDVWVRTGCLPGLSPEFDEIVKNERRERPELLKGRSNMTYHERKMAGLCPLTAMDVTRLLKALGAQKDARIYWAGGQPLGGKEALHPLIHEFPHLYNKEDLALPGELEPFSKKASLMAAIDYIVSEKSDVFMPSHGGNMGHALQGHRAYAGHKKYITPNKRQMHPYFLNSSLPEAEFNSIVKELHQNSLGQPELRTSKVGRDVTKYPVPECMCNDSHNDS*