AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0671590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0671590 MtrunA17_Chr4g0011061 89.597 298 31 0 1 298 1 298 0.0 516
Msa0671590 MtrunA17_Chr4g0056861 50.556 180 81 3 71 242 129 308 2.81e-47 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0671590 AT2G45380.3 51.460 274 112 7 33 298 15 275 3.52e-74 229
Msa0671590 AT2G45380.4 51.460 274 112 7 33 298 55 315 1.06e-73 229
Msa0671590 AT2G45380.1 51.460 274 112 6 33 298 234 494 1.40e-71 229
Msa0671590 AT2G45380.2 54.255 188 72 4 112 298 12 186 8.74e-51 166
Msa0671590 AT4G22740.1 42.353 255 92 7 31 233 47 298 2.47e-50 170
Msa0671590 AT4G22740.2 42.353 255 92 7 31 233 47 298 2.47e-50 170

Find 83 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGAGGGTTTGGAGATTT+TGG 0.152777 4_4:-15299670 Msa0671590:CDS
AACCTAATGCCTTGAGGTTT+TGG 0.208693 4_4:-15300217 None:intergenic
CCAGACGATGATGATGTTAA+TGG 0.273519 4_4:-15299391 Msa0671590:intron
GGTTTGGTCACTTTCATCTT+TGG 0.293434 4_4:-15297529 Msa0671590:CDS
CCCGTCAGTCCCCATCCTTC+TGG 0.315780 4_4:+15299005 None:intergenic
TTCCTTTGTATATCATAATC+AGG 0.321016 4_4:+15297945 None:intergenic
GCTTGGAAAGGCACTAATCT+AGG 0.344675 4_4:-15297975 Msa0671590:CDS
GAGAACACAAGCAGGGAAAA+AGG 0.346279 4_4:-15299508 Msa0671590:CDS
AAACCTCAACTCGATTTGAT+TGG 0.358053 4_4:+15297180 None:intergenic
TAAATACTTCATAGGCTCAT+AGG 0.372821 4_4:-15299836 Msa0671590:five_prime_UTR
CTCATAGGCTGAATATCAAC+AGG 0.373260 4_4:-15299821 Msa0671590:five_prime_UTR
AACCCTCGAAGGACCGTAAT+CGG 0.374410 4_4:-15299098 Msa0671590:CDS
CGTCAGTTGCACTTCAAATT+CGG 0.377279 4_4:-15300060 Msa0671590:five_prime_UTR
TTAGTGTTTAAATACTTCAT+AGG 0.387522 4_4:-15299844 Msa0671590:five_prime_UTR
GTAACATATGGTGGTATAGA+TGG 0.416298 4_4:-15299051 Msa0671590:CDS
AGTCCCCATCCTTCTGGTTC+TGG 0.416906 4_4:+15299011 None:intergenic
GAGCAAGATAGAAGAGAAAG+AGG 0.419817 4_4:-15297507 Msa0671590:CDS
GAAAAGACAAATTCCGATTA+CGG 0.422754 4_4:+15299085 None:intergenic
CAGACTAGCAAAGTAACATA+TGG 0.425363 4_4:-15299063 Msa0671590:CDS
GAAGAACAAAGAAAGTTGTT+AGG 0.437376 4_4:-15297446 Msa0671590:CDS
TATCGTTACAAACCTTATCA+TGG 0.443589 4_4:+15298818 None:intergenic
AAAGAAGCTGACACGACAAC+AGG 0.446251 4_4:-15298871 Msa0671590:CDS
GGATTCCTCAAGCTGTCAAA+CGG 0.446584 4_4:+15299565 None:intergenic
CCAGAAGGATGGGGACTGAC+GGG 0.454236 4_4:-15299005 Msa0671590:CDS
ACCTTCATAAAATAAAACTT+TGG 0.455620 4_4:-15300013 Msa0671590:five_prime_UTR
GTGATCCTGCTGGGCCATGA+GGG 0.466784 4_4:+15300121 None:intergenic
TTTAAGGATTGTGATCCTGC+TGG 0.468753 4_4:+15300111 None:intergenic
TGTGATCCTGCTGGGCCATG+AGG 0.475254 4_4:+15300120 None:intergenic
AATATTGCAGATTCTAGAGT+TGG 0.476430 4_4:-15297570 Msa0671590:intron
GATTCCTCAAGCTGTCAAAC+GGG 0.487934 4_4:+15299566 None:intergenic
CCATTAACATCATCATCGTC+TGG 0.487955 4_4:+15299391 None:intergenic
ATTCCGATTACGGTCCTTCG+AGG 0.488138 4_4:+15299095 None:intergenic
CTTGATTCAGACGGCAAGGT+TGG 0.488695 4_4:-15298148 Msa0671590:CDS
CACTAGAGGAATCCATGATA+AGG 0.488735 4_4:-15298830 Msa0671590:intron
ACTGAATCAGGTGGCGATTG+AGG 0.489439 4_4:-15298899 Msa0671590:intron
AGATGAGCTTACACACTTTG+AGG 0.492378 4_4:-15298000 Msa0671590:CDS
TGGTCTCTAACTTGCATATG+TGG 0.496882 4_4:-15300186 Msa0671590:five_prime_UTR
TTACACACTTTGAGGAAGCT+TGG 0.497309 4_4:-15297992 Msa0671590:CDS
TGTATATCATAATCAGGTAC+TGG 0.499045 4_4:+15297951 None:intergenic
TATGATATACAAAGGAAGAA+AGG 0.501569 4_4:-15297939 Msa0671590:intron
TAATATGAATTTCAAATCGA+GGG 0.502613 4_4:+15297213 None:intergenic
CTCACCCGTTTGACAGCTTG+AGG 0.506393 4_4:-15299570 Msa0671590:CDS
GATCTAGATGAAGTAGAACT+TGG 0.510217 4_4:+15299544 None:intergenic
TGATCCTGCTGGGCCATGAG+GGG 0.513732 4_4:+15300122 None:intergenic
TTAAGGATTGTGATCCTGCT+GGG 0.516819 4_4:+15300112 None:intergenic
ACGCTGCACAATCTTAATGA+AGG 0.518496 4_4:-15298115 Msa0671590:intron
GATCCTGCTGGGCCATGAGG+GGG 0.524045 4_4:+15300123 None:intergenic
AAATAAAACTTTGGATGATG+TGG 0.524772 4_4:-15300004 Msa0671590:five_prime_UTR
TTAGATAAAGAATATGCAGA+AGG 0.529750 4_4:-15299722 Msa0671590:exon
TAGATAAAGAATATGCAGAA+GGG 0.534133 4_4:-15299721 Msa0671590:exon
ACCAGAAGGATGGGGACTGA+CGG 0.534899 4_4:-15299006 Msa0671590:CDS
TTCCGATTACGGTCCTTCGA+GGG 0.541657 4_4:+15299096 None:intergenic
CTAATATGAATTTCAAATCG+AGG 0.543324 4_4:+15297212 None:intergenic
CTTGGAAAGGCACTAATCTA+GGG 0.548298 4_4:-15297974 Msa0671590:CDS
TCTACCAGAACCAGAAGGAT+GGG 0.564337 4_4:-15299015 Msa0671590:CDS
AATCCAATCAAATCGAGTTG+AGG 0.566824 4_4:-15297183 Msa0671590:three_prime_UTR
TTGAGGAAGCTTGATTCAGA+CGG 0.567117 4_4:-15298157 Msa0671590:CDS
TTCTACCAGAACCAGAAGGA+TGG 0.573020 4_4:-15299016 Msa0671590:CDS
CACTTTGAGGAAGCTTGGAA+AGG 0.574132 4_4:-15297987 Msa0671590:CDS
AGAAGACTGAGAACACAAGC+AGG 0.574200 4_4:-15299516 Msa0671590:CDS
TCGTCTGGATGCTCAACAGA+TGG 0.576041 4_4:+15299406 None:intergenic
AGTATGTAAAATGTAGAATG+AGG 0.580460 4_4:-15297373 Msa0671590:three_prime_UTR
TATGAAAGTCGAAACAAATG+TGG 0.593107 4_4:-15297471 Msa0671590:CDS
CAATATTGAGTGAATTGTGT+AGG 0.597629 4_4:-15297421 Msa0671590:exon
AGAATTAGACTCTGATGATG+AGG 0.598339 4_4:-15299473 Msa0671590:CDS
ACTAGATTGGATCATCACTT+CGG 0.604341 4_4:+15300152 None:intergenic
CTAGCAGAGAGGAAAGGCAG+TGG 0.608241 4_4:-15299156 Msa0671590:intron
CAGAAGGATGGGGACTGACG+GGG 0.612887 4_4:-15299004 Msa0671590:intron
GAAGCTTGATTCAGACGGCA+AGG 0.613499 4_4:-15298152 Msa0671590:CDS
TGAGTACTAGCAGAGAGGAA+AGG 0.624923 4_4:-15299162 Msa0671590:intron
AGCTGACACGACAACAGGCG+AGG 0.627903 4_4:-15298866 Msa0671590:CDS
GAATGAGGTGTATTGTTGTG+CGG 0.631430 4_4:-15297358 Msa0671590:three_prime_UTR
GAAAGTCGAAACAAATGTGG+AGG 0.633362 4_4:-15297468 Msa0671590:CDS
CTACCAGAACCAGAAGGATG+GGG 0.636130 4_4:-15299014 Msa0671590:CDS
TACCTGATTATGATATACAA+AGG 0.642517 4_4:-15297947 Msa0671590:CDS
ACTAGCAAAGTAACATATGG+TGG 0.655228 4_4:-15299060 Msa0671590:CDS
ATACTTCTACCAGAACCAGA+AGG 0.666727 4_4:-15299020 Msa0671590:CDS
GAAGACTGAGAACACAAGCA+GGG 0.671813 4_4:-15299515 Msa0671590:CDS
AGTGATGATCCAATCTAGTG+AGG 0.673139 4_4:-15300148 Msa0671590:five_prime_UTR
CAAATCAGAAGAACCCTCGA+AGG 0.686390 4_4:-15299109 Msa0671590:CDS
GCAACACATAGAATCACTAG+AGG 0.698772 4_4:-15298844 Msa0671590:CDS
TGAGTGAATTGTGTAGGACA+TGG 0.729356 4_4:-15297415 Msa0671590:exon
TCATAGGCTGAATATCAACA+GGG 0.751976 4_4:-15299820 Msa0671590:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTTATTAAAAATTTA+AGG + chr4_4:15297459-15297478 None:intergenic 0.0%
!!! ACATTTTTTTGACAATTTTA+AGG + chr4_4:15297286-15297305 None:intergenic 15.0%
!!! ATCTTATCATTATACTATTT+TGG - chr4_4:15298058-15298077 Msa0671590:intron 15.0%
!! TTGAACTTATTTATTTAGTT+TGG - chr4_4:15298167-15298186 Msa0671590:CDS 15.0%
!! AAAAAATGTCAAATTATGTA+TGG - chr4_4:15298672-15298691 Msa0671590:intron 15.0%
!! TTAAATGAAGTAAACAATTT+CGG + chr4_4:15299558-15299577 None:intergenic 15.0%
!! AATTGTTTACTTCATTTAAA+TGG - chr4_4:15299559-15299578 Msa0671590:CDS 15.0%
!! ATTGTTTACTTCATTTAAAT+GGG - chr4_4:15299560-15299579 Msa0671590:CDS 15.0%
!! ACCTTCATAAAATAAAACTT+TGG - chr4_4:15297365-15297384 Msa0671590:three_prime_UTR 20.0%
!!! TCCAAAGTTTTATTTTATGA+AGG + chr4_4:15297369-15297388 None:intergenic 20.0%
!! TTAGTGTTTAAATACTTCAT+AGG - chr4_4:15297534-15297553 Msa0671590:CDS 20.0%
!!! CAATAGAAGTTTTTTTATCA+CGG - chr4_4:15298023-15298042 Msa0671590:intron 20.0%
!!! TATTTAGTTTGGTTCATATA+TGG - chr4_4:15298178-15298197 Msa0671590:intron 20.0%
!! GTTAATATTTAAGGAAATGA+AGG - chr4_4:15298993-15299012 Msa0671590:intron 20.0%
!! TTAGTTAGTTGATTAGAAAA+GGG - chr4_4:15299034-15299053 Msa0671590:CDS 20.0%
!!! GATAACAATGTCTTATTTAT+AGG + chr4_4:15299098-15299117 None:intergenic 20.0%
!! TTCTCTGTTATTGTATAATA+AGG - chr4_4:15299126-15299145 Msa0671590:CDS 20.0%
!! AACAAAAAAATGATAGGAAT+CGG + chr4_4:15299154-15299173 None:intergenic 20.0%
!!! TCCTATCATTTTTTTGTTTT+AGG - chr4_4:15299156-15299175 Msa0671590:intron 20.0%
!! ACCTAAAACAAAAAAATGAT+AGG + chr4_4:15299160-15299179 None:intergenic 20.0%
!!! TTTTTTTGTTTTAGGTATTG+AGG - chr4_4:15299164-15299183 Msa0671590:intron 20.0%
!! ATTGTACGATTTCAAAATAA+TGG - chr4_4:15299532-15299551 Msa0671590:CDS 20.0%
!! ATTGTGTTACAAATTAAGTT+AGG + chr4_4:15299591-15299610 None:intergenic 20.0%
!!! TATACACTATCTTCATTTTT+TGG - chr4_4:15299668-15299687 Msa0671590:CDS 20.0%
!!! ATACACTATCTTCATTTTTT+GGG - chr4_4:15299669-15299688 Msa0671590:CDS 20.0%
!!! CTATTATATGTTTGTTTTCT+TGG - chr4_4:15300079-15300098 Msa0671590:five_prime_UTR 20.0%
!! TAATATGAATTTCAAATCGA+GGG + chr4_4:15300168-15300187 None:intergenic 20.0%
! AAATAAAACTTTGGATGATG+TGG - chr4_4:15297374-15297393 Msa0671590:three_prime_UTR 25.0%
!!! TTCTAAGTTTTTTGACATGA+TGG - chr4_4:15297590-15297609 Msa0671590:intron 25.0%
!!! ATGGTTTGTTGTGTTTTATT+TGG - chr4_4:15297609-15297628 Msa0671590:intron 25.0%
! TTAGATAAAGAATATGCAGA+AGG - chr4_4:15297656-15297675 Msa0671590:intron 25.0%
! TAGATAAAGAATATGCAGAA+GGG - chr4_4:15297657-15297676 Msa0671590:intron 25.0%
!! ACAATGTTTTCAAATCAAGA+GGG - chr4_4:15297694-15297713 Msa0671590:intron 25.0%
! CTATATGATATAACTGAATC+AGG - chr4_4:15298467-15298486 Msa0671590:intron 25.0%
! TGTTAAGTGTAGATGAAATA+AGG - chr4_4:15298623-15298642 Msa0671590:intron 25.0%
! AAAGAAAAACCTTCTACATT+AGG - chr4_4:15298649-15298668 Msa0671590:intron 25.0%
!!! ACATTTTTTCCTAATGTAGA+AGG + chr4_4:15298661-15298680 None:intergenic 25.0%
! AACCAGGTTGTTAATATTTA+AGG - chr4_4:15298984-15299003 Msa0671590:intron 25.0%
! TTCCTTAAATATTAACAACC+TGG + chr4_4:15298989-15299008 None:intergenic 25.0%
! CTTAGTTAGTTGATTAGAAA+AGG - chr4_4:15299033-15299052 Msa0671590:CDS 25.0%
! TGATTAGAAAAGGGTTAATT+AGG - chr4_4:15299043-15299062 Msa0671590:CDS 25.0%
! TTAAGTTTCTATATGTATGC+AGG - chr4_4:15299187-15299206 Msa0671590:intron 25.0%
! TAAGTTTCTATATGTATGCA+GGG - chr4_4:15299188-15299207 Msa0671590:intron 25.0%
! GTGGTAAAAAGAAATAATGA+TGG - chr4_4:15299332-15299351 Msa0671590:intron 25.0%
! TACCTGATTATGATATACAA+AGG - chr4_4:15299431-15299450 Msa0671590:CDS 25.0%
! TTCCTTTGTATATCATAATC+AGG + chr4_4:15299436-15299455 None:intergenic 25.0%
! TATGATATACAAAGGAAGAA+AGG - chr4_4:15299439-15299458 Msa0671590:CDS 25.0%
! ACAATAAGAAACTCAAATTG+AGG + chr4_4:15299517-15299536 None:intergenic 25.0%
! GATTCTACATTAAACAAACA+TGG + chr4_4:15299732-15299751 None:intergenic 25.0%
! AATGTAGAATCATCATTCTT+CGG - chr4_4:15299741-15299760 Msa0671590:five_prime_UTR 25.0%
! AGTATGTAAAATGTAGAATG+AGG - chr4_4:15300005-15300024 Msa0671590:five_prime_UTR 25.0%
! CTAATATGAATTTCAAATCG+AGG + chr4_4:15300169-15300188 None:intergenic 25.0%
TAAATACTTCATAGGCTCAT+AGG - chr4_4:15297542-15297561 Msa0671590:CDS 30.0%
! GACAATGTTTTCAAATCAAG+AGG - chr4_4:15297693-15297712 Msa0671590:intron 30.0%
! GTCTTGATGAGAAAATTTTG+AGG - chr4_4:15297937-15297956 Msa0671590:intron 30.0%
!!! GAAAATTTTGAGGTCATCAA+TGG - chr4_4:15297947-15297966 Msa0671590:CDS 30.0%
! TTTTATCACGGTTAATCGAT+CGG - chr4_4:15298035-15298054 Msa0671590:intron 30.0%
GAAAAGACAAATTCCGATTA+CGG + chr4_4:15298296-15298315 None:intergenic 30.0%
TTGAGACAATTAACACAAAG+AGG + chr4_4:15298422-15298441 None:intergenic 30.0%
TATGATATAACTGAATCAGG+TGG - chr4_4:15298470-15298489 Msa0671590:intron 30.0%
TATCGTTACAAACCTTATCA+TGG + chr4_4:15298563-15298582 None:intergenic 30.0%
GATAAGGTTTGTAACGATAT+AGG - chr4_4:15298564-15298583 Msa0671590:intron 30.0%
! TTTTCATATGCTCTCCAAAA+CGG - chr4_4:15298915-15298934 Msa0671590:intron 30.0%
TCAAATAAGCTAATTCAACC+AGG - chr4_4:15298968-15298987 Msa0671590:intron 30.0%
TGTATATCATAATCAGGTAC+TGG + chr4_4:15299430-15299449 None:intergenic 30.0%
CACAAAGGGAACAAAAAAAA+AGG + chr4_4:15299469-15299488 None:intergenic 30.0%
AGCATCAATATTCACACAAA+GGG + chr4_4:15299483-15299502 None:intergenic 30.0%
AAGCATCAATATTCACACAA+AGG + chr4_4:15299484-15299503 None:intergenic 30.0%
TAAGAAACTCAAATTGAGGA+AGG + chr4_4:15299513-15299532 None:intergenic 30.0%
!! AATATTGCAGATTCTAGAGT+TGG - chr4_4:15299808-15299827 Msa0671590:five_prime_UTR 30.0%
AGCTAAACAAAAACAAGGTT+TGG - chr4_4:15299833-15299852 Msa0671590:five_prime_UTR 30.0%
TATGAAAGTCGAAACAAATG+TGG - chr4_4:15299907-15299926 Msa0671590:five_prime_UTR 30.0%
GAAGAACAAAGAAAGTTGTT+AGG - chr4_4:15299932-15299951 Msa0671590:five_prime_UTR 30.0%
CAATATTGAGTGAATTGTGT+AGG - chr4_4:15299957-15299976 Msa0671590:five_prime_UTR 30.0%
ACTAGATTGGATCATCACTT+CGG + chr4_4:15297229-15297248 None:intergenic 35.0%
TCATAGGCTGAATATCAACA+GGG - chr4_4:15297558-15297577 Msa0671590:CDS 35.0%
! GTTTTCAAATCAAGAGGGTT+TGG - chr4_4:15297699-15297718 Msa0671590:intron 35.0%
!!! TTTGGAGATTTTGGTGGATT+TGG - chr4_4:15297717-15297736 Msa0671590:intron 35.0%
GTGAAAAAAGGATCGTCAAA+TGG + chr4_4:15297789-15297808 None:intergenic 35.0%
GATCTAGATGAAGTAGAACT+TGG + chr4_4:15297837-15297856 None:intergenic 35.0%
! AGAATTAGACTCTGATGATG+AGG - chr4_4:15297905-15297924 Msa0671590:intron 35.0%
! TTTGCTTGACAAGTTCTGAT+TGG - chr4_4:15298103-15298122 Msa0671590:intron 35.0%
CAGACTAGCAAAGTAACATA+TGG - chr4_4:15298315-15298334 Msa0671590:intron 35.0%
ACTAGCAAAGTAACATATGG+TGG - chr4_4:15298318-15298337 Msa0671590:intron 35.0%
GTAACATATGGTGGTATAGA+TGG - chr4_4:15298327-15298346 Msa0671590:intron 35.0%
TATATGTTCAACAGTTGCTC+AGG - chr4_4:15298709-15298728 Msa0671590:intron 35.0%
TAACACACTAGTCAATCAAC+AGG + chr4_4:15298739-15298758 None:intergenic 35.0%
! CTAGTGTGTTAGCTTCTATT+TGG - chr4_4:15298748-15298767 Msa0671590:intron 35.0%
TCACTAGTGGTTATGTAAGT+AGG - chr4_4:15298940-15298959 Msa0671590:intron 35.0%
TTGTTTAACAACATCTCGAG+TGG - chr4_4:15299313-15299332 Msa0671590:intron 35.0%
!!! TTTTTGGGTGCAACTTAGAT+TGG - chr4_4:15299684-15299703 Msa0671590:CDS 35.0%
TGGTGAGCTAAACAAAAACA+AGG - chr4_4:15299828-15299847 Msa0671590:five_prime_UTR 35.0%
! AATCCAATCAAATCGAGTTG+AGG - chr4_4:15300195-15300214 Msa0671590:five_prime_UTR 35.0%
! AAACCTCAACTCGATTTGAT+TGG + chr4_4:15300201-15300220 None:intergenic 35.0%
! TGGTCTCTAACTTGCATATG+TGG - chr4_4:15297192-15297211 Msa0671590:three_prime_UTR 40.0%
AGTGATGATCCAATCTAGTG+AGG - chr4_4:15297230-15297249 Msa0671590:three_prime_UTR 40.0%
TTAAGGATTGTGATCCTGCT+GGG + chr4_4:15297269-15297288 None:intergenic 40.0%
TTTAAGGATTGTGATCCTGC+TGG + chr4_4:15297270-15297289 None:intergenic 40.0%
CGTCAGTTGCACTTCAAATT+CGG - chr4_4:15297318-15297337 Msa0671590:three_prime_UTR 40.0%
CTCATAGGCTGAATATCAAC+AGG - chr4_4:15297557-15297576 Msa0671590:CDS 40.0%
! TCAAGAGGGTTTGGAGATTT+TGG - chr4_4:15297708-15297727 Msa0671590:intron 40.0%
!! TAGGCATCGTCATACTTTTG+TGG + chr4_4:15297746-15297765 None:intergenic 40.0%
!! ATGACGATGCCTAGTCTTTT+TGG - chr4_4:15297753-15297772 Msa0671590:intron 40.0%
TCTCTTCCTCCAAAAAGACT+AGG + chr4_4:15297765-15297784 None:intergenic 40.0%
TCAAACGGGTGAGTGAAAAA+AGG + chr4_4:15297801-15297820 None:intergenic 40.0%
CCATTAACATCATCATCGTC+TGG + chr4_4:15297990-15298009 None:intergenic 40.0%
! CCAGACGATGATGATGTTAA+TGG - chr4_4:15297987-15298006 Msa0671590:CDS 40.0%
ATACTTCTACCAGAACCAGA+AGG - chr4_4:15298358-15298377 Msa0671590:intron 40.0%
GCAACACATAGAATCACTAG+AGG - chr4_4:15298534-15298553 Msa0671590:intron 40.0%
CACTAGAGGAATCCATGATA+AGG - chr4_4:15298548-15298567 Msa0671590:intron 40.0%
AAGCTATCGTAGAGAGCTTA+TGG - chr4_4:15298877-15298896 Msa0671590:CDS 40.0%
GGAAATGAAGGATCTGTGAT+CGG - chr4_4:15299005-15299024 Msa0671590:CDS 40.0%
TTGAGGAAGCTTGATTCAGA+CGG - chr4_4:15299221-15299240 Msa0671590:intron 40.0%
ACGCTGCACAATCTTAATGA+AGG - chr4_4:15299263-15299282 Msa0671590:intron 40.0%
AGATGAGCTTACACACTTTG+AGG - chr4_4:15299378-15299397 Msa0671590:intron 40.0%
! TTACACACTTTGAGGAAGCT+TGG - chr4_4:15299386-15299405 Msa0671590:intron 40.0%
!! CTTGGAAAGGCACTAATCTA+GGG - chr4_4:15299404-15299423 Msa0671590:CDS 40.0%
! GGTTTGGTCACTTTCATCTT+TGG - chr4_4:15299849-15299868 Msa0671590:five_prime_UTR 40.0%
GAGCAAGATAGAAGAGAAAG+AGG - chr4_4:15299871-15299890 Msa0671590:five_prime_UTR 40.0%
GAAAGTCGAAACAAATGTGG+AGG - chr4_4:15299910-15299929 Msa0671590:five_prime_UTR 40.0%
TGAGTGAATTGTGTAGGACA+TGG - chr4_4:15299963-15299982 Msa0671590:five_prime_UTR 40.0%
GAATGAGGTGTATTGTTGTG+CGG - chr4_4:15300020-15300039 Msa0671590:five_prime_UTR 40.0%
! AGAGGGTTTGGAGATTTTGG+TGG - chr4_4:15297711-15297730 Msa0671590:intron 45.0%
!! ACGATGCCTAGTCTTTTTGG+AGG - chr4_4:15297756-15297775 Msa0671590:intron 45.0%
GATTCCTCAAGCTGTCAAAC+GGG + chr4_4:15297815-15297834 None:intergenic 45.0%
GGATTCCTCAAGCTGTCAAA+CGG + chr4_4:15297816-15297835 None:intergenic 45.0%
AGAAGACTGAGAACACAAGC+AGG - chr4_4:15297862-15297881 Msa0671590:intron 45.0%
GAAGACTGAGAACACAAGCA+GGG - chr4_4:15297863-15297882 Msa0671590:intron 45.0%
GAGAACACAAGCAGGGAAAA+AGG - chr4_4:15297870-15297889 Msa0671590:intron 45.0%
TGAGTACTAGCAGAGAGGAA+AGG - chr4_4:15298216-15298235 Msa0671590:intron 45.0%
CAAATCAGAAGAACCCTCGA+AGG - chr4_4:15298269-15298288 Msa0671590:intron 45.0%
TTCTACCAGAACCAGAAGGA+TGG - chr4_4:15298362-15298381 Msa0671590:intron 45.0%
TCTACCAGAACCAGAAGGAT+GGG - chr4_4:15298363-15298382 Msa0671590:intron 45.0%
TAACACAAAGAGGAAGTCGC+AGG + chr4_4:15298412-15298431 None:intergenic 45.0%
AAAGAAGCTGACACGACAAC+AGG - chr4_4:15298507-15298526 Msa0671590:intron 45.0%
! AACCACTAGTGAGACCGTTT+TGG + chr4_4:15298932-15298951 None:intergenic 45.0%
! TGCAGGGTCATTCAGTTTTG+AGG - chr4_4:15299204-15299223 Msa0671590:intron 45.0%
! CACTTTGAGGAAGCTTGGAA+AGG - chr4_4:15299391-15299410 Msa0671590:intron 45.0%
!! GCTTGGAAAGGCACTAATCT+AGG - chr4_4:15299403-15299422 Msa0671590:CDS 45.0%
!!! TTTTTAATAAAAATATAATG+TGG - chr4_4:15297463-15297482 Msa0671590:CDS 5.0%
CACAAGCAGGGAAAAAGGAG+TGG - chr4_4:15297875-15297894 Msa0671590:intron 50.0%
TCGTCTGGATGCTCAACAGA+TGG + chr4_4:15297975-15297994 None:intergenic 50.0%
TCGTGTGAGTACTAGCAGAG+AGG - chr4_4:15298211-15298230 Msa0671590:intron 50.0%
AACCCTCGAAGGACCGTAAT+CGG - chr4_4:15298280-15298299 Msa0671590:intron 50.0%
TTCCGATTACGGTCCTTCGA+GGG + chr4_4:15298285-15298304 None:intergenic 50.0%
ATTCCGATTACGGTCCTTCG+AGG + chr4_4:15298286-15298305 None:intergenic 50.0%
CTACCAGAACCAGAAGGATG+GGG - chr4_4:15298364-15298383 Msa0671590:intron 50.0%
ACTGAATCAGGTGGCGATTG+AGG - chr4_4:15298479-15298498 Msa0671590:intron 50.0%
CCACTGCAGAAATGCGTTCT+TGG + chr4_4:15298591-15298610 None:intergenic 50.0%
CCAAGAACGCATTTCTGCAG+TGG - chr4_4:15298588-15298607 Msa0671590:intron 50.0%
CTCCAAAACGGTCTCACTAG+TGG - chr4_4:15298927-15298946 Msa0671590:intron 50.0%
GAAGCTTGATTCAGACGGCA+AGG - chr4_4:15299226-15299245 Msa0671590:intron 50.0%
CTTGATTCAGACGGCAAGGT+TGG - chr4_4:15299230-15299249 Msa0671590:intron 50.0%
CTCACCCGTTTGACAGCTTG+AGG - chr4_4:15297808-15297827 Msa0671590:intron 55.0%
CTAGCAGAGAGGAAAGGCAG+TGG - chr4_4:15298222-15298241 Msa0671590:intron 55.0%
!! AGTCCCCATCCTTCTGGTTC+TGG + chr4_4:15298370-15298389 None:intergenic 55.0%
ACCAGAAGGATGGGGACTGA+CGG - chr4_4:15298372-15298391 Msa0671590:intron 55.0%
GAGGGGGAGCCTCACTAGAT+TGG + chr4_4:15297242-15297261 None:intergenic 60.0%
TCTAGTGAGGCTCCCCCTCA+TGG - chr4_4:15297243-15297262 Msa0671590:three_prime_UTR 60.0%
TGATCCTGCTGGGCCATGAG+GGG + chr4_4:15297259-15297278 None:intergenic 60.0%
GTGATCCTGCTGGGCCATGA+GGG + chr4_4:15297260-15297279 None:intergenic 60.0%
TGTGATCCTGCTGGGCCATG+AGG + chr4_4:15297261-15297280 None:intergenic 60.0%
CCAGAAGGATGGGGACTGAC+GGG - chr4_4:15298373-15298392 Msa0671590:intron 60.0%
CAGAAGGATGGGGACTGACG+GGG - chr4_4:15298374-15298393 Msa0671590:intron 60.0%
AGCTGACACGACAACAGGCG+AGG - chr4_4:15298512-15298531 Msa0671590:intron 60.0%
GATCCTGCTGGGCCATGAGG+GGG + chr4_4:15297258-15297277 None:intergenic 65.0%
CCCGTCAGTCCCCATCCTTC+TGG + chr4_4:15298376-15298395 None:intergenic 65.0%
GCTCCCCCTCATGGCCCAGC+AGG - chr4_4:15297252-15297271 Msa0671590:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 15297174 15300226 15297174 ID=Msa0671590;Name=Msa0671590
chr4_4 mRNA 15297174 15300226 15297174 ID=Msa0671590-mRNA-1;Parent=Msa0671590;Name=Msa0671590-mRNA-1;_AED=0.11;_eAED=0.11;_QI=495|1|1|1|1|1|6|257|298
chr4_4 exon 15297174 15297582 15297174 ID=Msa0671590-mRNA-1:exon:4628;Parent=Msa0671590-mRNA-1
chr4_4 exon 15297940 15298020 15297940 ID=Msa0671590-mRNA-1:exon:4627;Parent=Msa0671590-mRNA-1
chr4_4 exon 15298116 15298191 15298116 ID=Msa0671590-mRNA-1:exon:4626;Parent=Msa0671590-mRNA-1
chr4_4 exon 15298831 15298911 15298831 ID=Msa0671590-mRNA-1:exon:4625;Parent=Msa0671590-mRNA-1
chr4_4 exon 15299005 15299171 15299005 ID=Msa0671590-mRNA-1:exon:4624;Parent=Msa0671590-mRNA-1
chr4_4 exon 15299392 15300226 15299392 ID=Msa0671590-mRNA-1:exon:4623;Parent=Msa0671590-mRNA-1
chr4_4 five_prime_UTR 15299732 15300226 15299732 ID=Msa0671590-mRNA-1:five_prime_utr;Parent=Msa0671590-mRNA-1
chr4_4 CDS 15299392 15299731 15299392 ID=Msa0671590-mRNA-1:cds;Parent=Msa0671590-mRNA-1
chr4_4 CDS 15299005 15299171 15299005 ID=Msa0671590-mRNA-1:cds;Parent=Msa0671590-mRNA-1
chr4_4 CDS 15298831 15298911 15298831 ID=Msa0671590-mRNA-1:cds;Parent=Msa0671590-mRNA-1
chr4_4 CDS 15298116 15298191 15298116 ID=Msa0671590-mRNA-1:cds;Parent=Msa0671590-mRNA-1
chr4_4 CDS 15297940 15298020 15297940 ID=Msa0671590-mRNA-1:cds;Parent=Msa0671590-mRNA-1
chr4_4 CDS 15297431 15297582 15297431 ID=Msa0671590-mRNA-1:cds;Parent=Msa0671590-mRNA-1
chr4_4 three_prime_UTR 15297174 15297430 15297174 ID=Msa0671590-mRNA-1:three_prime_utr;Parent=Msa0671590-mRNA-1
Gene Sequence

>Msa0671590

ATGCAGAAGGGTAGAGAAGTTGAAGACAATGTTTTCAAATCAAGAGGGTTTGGAGATTTTGGTGGATTTGGCTTCCACAAAAGTATGACGATGCCTAGTCTTTTTGGAGGAAGAGATCCATTTGACGATCCTTTTTTCACTCACCCGTTTGACAGCTTGAGGAATCCAAGTTCTACTTCATCTAGATCAATGCAGAAGACTGAGAACACAAGCAGGGAAAAAGGAGTGGTTATAAAAGAATTAGACTCTGATGATGAGGACAATGATAGTCTTGATGAGAAAATTTTGAGGTCATCAATGGAGCCATCTGTTGAGCATCCAGACGATGATGATGTTAATGAGAGGAAAGGCAGTGGCATGACTCACAAGATTGATCTTCACAAATCAGAAGAACCCTCGAAGGACCGTAATCGGAATTTGTCTTTTCAGACTAGCAAAGTAACATATGGTGGTATAGATGGTGCTTACTATACTTCTACCAGAACCAGAAGGATGGGGACTGACGGGGTGGCGATTGAGGAAAGCAAAGAAGCTGACACGACAACAGGCGAGGCAACACATAGAATCACTAGAGGAATCCATGATAAGGGTCATTCAGTTTTGAGGAAGCTTGATTCAGACGGCAAGGTTGGTACAACACAAACGCTGCACAATCTTAATGAAGATGAGCTTACACACTTTGAGGAAGCTTGGAAAGGCACTAATCTAGGGCCAGTACCTGATTATGATATACAAAGGAAGAAAGATTCTAGAGTTGGTGAGCTAAACAAAAACAAGGTTTGGTCACTTTCATCTTTGGAGCAAGATAGAAGAGAAAGAGGATTTTCATCAAATTATGAAAGTCGAAACAAATGTGGAGGAAGAACAAAGAAAGTTGTTAGGATCAATATTGAGTGA
Protein sequence

>Msa0671590

MQKGREVEDNVFKSRGFGDFGGFGFHKSMTMPSLFGGRDPFDDPFFTHPFDSLRNPSSTSSRSMQKTENTSREKGVVIKELDSDDEDNDSLDEKILRSSMEPSVEHPDDDDVNERKGSGMTHKIDLHKSEEPSKDRNRNLSFQTSKVTYGGIDGAYYTSTRTRRMGTDGVAIEESKEADTTTGEATHRITRGIHDKGHSVLRKLDSDGKVGTTQTLHNLNEDELTHFEEAWKGTNLGPVPDYDIQRKKDSRVGELNKNKVWSLSSLEQDRRERGFSSNYESRNKCGGRTKKVVRINIE*