AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1428390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1428390 MtrunA17_Chr4g0011061 89.597 298 31 0 1 298 1 298 0.0 516
Msa1428390 MtrunA17_Chr4g0056861 50.556 180 81 3 71 242 129 308 2.81e-47 162
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1428390 AT2G45380.3 51.460 274 112 7 33 298 15 275 3.52e-74 229
Msa1428390 AT2G45380.4 51.460 274 112 7 33 298 55 315 1.06e-73 229
Msa1428390 AT2G45380.1 51.460 274 112 6 33 298 234 494 1.40e-71 229
Msa1428390 AT2G45380.2 54.255 188 72 4 112 298 12 186 8.74e-51 166
Msa1428390 AT4G22740.1 42.353 255 92 7 31 233 47 298 2.47e-50 170
Msa1428390 AT4G22740.2 42.353 255 92 7 31 233 47 298 2.47e-50 170

Find 84 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGAGGGTTTGGAGATTT+TGG 0.152777 tig0026340:+37860 Msa1428390:CDS
CCAGACGATGATGATGTTAA+TGG 0.273519 tig0026340:+38139 Msa1428390:CDS
GGTTTGGTCACTTTCATCTT+TGG 0.293434 tig0026340:+40001 Msa1428390:CDS
CCCGTCAGTCCCCATCCTTC+TGG 0.315780 tig0026340:-38525 None:intergenic
TTCCTTTGTATATCATAATC+AGG 0.321016 tig0026340:-39585 None:intergenic
GCTTGGAAAGGCACTAATCT+AGG 0.344675 tig0026340:+39555 Msa1428390:CDS
GAGAACACAAGCAGGGAAAA+AGG 0.346279 tig0026340:+38022 Msa1428390:CDS
AAACCTCAACTCGATTTGAT+TGG 0.358053 tig0026340:-40352 None:intergenic
TAAATACTTCATAGGCTCAT+AGG 0.372821 tig0026340:+37694 Msa1428390:five_prime_UTR
CTCATAGGCTGAATATCAAC+AGG 0.373260 tig0026340:+37709 Msa1428390:five_prime_UTR
AACCCTCGAAGGACCGTAAT+CGG 0.374410 tig0026340:+38432 Msa1428390:CDS
CGTCAGTTGCACTTCAAATT+CGG 0.377279 tig0026340:+37472 Msa1428390:five_prime_UTR
TTAGTGTTTAAATACTTCAT+AGG 0.387522 tig0026340:+37686 Msa1428390:five_prime_UTR
AAACCTAATGCCTTGAGGTT+TGG 0.393951 tig0026340:+37315 None:intergenic
GTAACATATGGTGGTATAGA+TGG 0.416298 tig0026340:+38479 Msa1428390:CDS
CTTGAGGTTTGGTGAAGATC+TGG 0.416626 tig0026340:+37326 None:intergenic
AGTCCCCATCCTTCTGGTTC+TGG 0.416906 tig0026340:-38519 None:intergenic
GAGCAAGATAGAAGAGAAAG+AGG 0.419817 tig0026340:+40023 Msa1428390:CDS
GAAAAGACAAATTCCGATTA+CGG 0.422754 tig0026340:-38445 None:intergenic
CAGACTAGCAAAGTAACATA+TGG 0.425363 tig0026340:+38467 Msa1428390:CDS
GAAGAACAAAGAAAGTTGTT+AGG 0.437376 tig0026340:+40084 Msa1428390:CDS
TATCGTTACAAACCTTATCA+TGG 0.443589 tig0026340:-38712 None:intergenic
AAAGAAGCTGACACGACAAC+AGG 0.446251 tig0026340:+38659 Msa1428390:CDS
GGATTCCTCAAGCTGTCAAA+CGG 0.446584 tig0026340:-37965 None:intergenic
CCAGAAGGATGGGGACTGAC+GGG 0.454236 tig0026340:+38525 Msa1428390:CDS
ACCTTCATAAAATAAAACTT+TGG 0.455620 tig0026340:+37519 Msa1428390:five_prime_UTR
GTGATCCTGCTGGGCCATGA+GGG 0.466784 tig0026340:-37411 None:intergenic
TTTAAGGATTGTGATCCTGC+TGG 0.468753 tig0026340:-37421 None:intergenic
TGTGATCCTGCTGGGCCATG+AGG 0.475254 tig0026340:-37412 None:intergenic
AATATTGCAGATTCTAGAGT+TGG 0.476430 tig0026340:+39960 Msa1428390:intron
GATTCCTCAAGCTGTCAAAC+GGG 0.487934 tig0026340:-37964 None:intergenic
CCATTAACATCATCATCGTC+TGG 0.487955 tig0026340:-38139 None:intergenic
ATTCCGATTACGGTCCTTCG+AGG 0.488138 tig0026340:-38435 None:intergenic
CTTGATTCAGACGGCAAGGT+TGG 0.488695 tig0026340:+39382 Msa1428390:CDS
CACTAGAGGAATCCATGATA+AGG 0.488735 tig0026340:+38700 Msa1428390:CDS
ACTGAATCAGGTGGCGATTG+AGG 0.489439 tig0026340:+38631 Msa1428390:intron
AGATGAGCTTACACACTTTG+AGG 0.492378 tig0026340:+39530 Msa1428390:intron
TGGTCTCTAACTTGCATATG+TGG 0.496882 tig0026340:+37346 Msa1428390:five_prime_UTR
TTACACACTTTGAGGAAGCT+TGG 0.497309 tig0026340:+39538 Msa1428390:CDS
TGTATATCATAATCAGGTAC+TGG 0.499045 tig0026340:-39579 None:intergenic
TATGATATACAAAGGAAGAA+AGG 0.501569 tig0026340:+39591 Msa1428390:CDS
TAATATGAATTTCAAATCGA+GGG 0.502613 tig0026340:-40319 None:intergenic
CTCACCCGTTTGACAGCTTG+AGG 0.506393 tig0026340:+37960 Msa1428390:CDS
GATCTAGATGAAGTAGAACT+TGG 0.510217 tig0026340:-37986 None:intergenic
TGATCCTGCTGGGCCATGAG+GGG 0.513732 tig0026340:-37410 None:intergenic
TTAAGGATTGTGATCCTGCT+GGG 0.516819 tig0026340:-37420 None:intergenic
ACGCTGCACAATCTTAATGA+AGG 0.518496 tig0026340:+39415 Msa1428390:CDS
GATCCTGCTGGGCCATGAGG+GGG 0.524045 tig0026340:-37409 None:intergenic
AAATAAAACTTTGGATGATG+TGG 0.524772 tig0026340:+37528 Msa1428390:five_prime_UTR
TTAGATAAAGAATATGCAGA+AGG 0.529750 tig0026340:+37808 Msa1428390:exon
TAGATAAAGAATATGCAGAA+GGG 0.534133 tig0026340:+37809 Msa1428390:exon
ACCAGAAGGATGGGGACTGA+CGG 0.534899 tig0026340:+38524 Msa1428390:CDS
TTCCGATTACGGTCCTTCGA+GGG 0.541657 tig0026340:-38434 None:intergenic
CTAATATGAATTTCAAATCG+AGG 0.543324 tig0026340:-40320 None:intergenic
CTTGGAAAGGCACTAATCTA+GGG 0.548298 tig0026340:+39556 Msa1428390:CDS
TCTACCAGAACCAGAAGGAT+GGG 0.564337 tig0026340:+38515 Msa1428390:CDS
AATCCAATCAAATCGAGTTG+AGG 0.566824 tig0026340:+40349 Msa1428390:three_prime_UTR
TTGAGGAAGCTTGATTCAGA+CGG 0.567117 tig0026340:+39373 Msa1428390:CDS
TTCTACCAGAACCAGAAGGA+TGG 0.573020 tig0026340:+38514 Msa1428390:CDS
CACTTTGAGGAAGCTTGGAA+AGG 0.574132 tig0026340:+39543 Msa1428390:CDS
AGAAGACTGAGAACACAAGC+AGG 0.574200 tig0026340:+38014 Msa1428390:CDS
TCGTCTGGATGCTCAACAGA+TGG 0.576041 tig0026340:-38124 None:intergenic
AGTATGTAAAATGTAGAATG+AGG 0.580460 tig0026340:+40157 Msa1428390:three_prime_UTR
TATGAAAGTCGAAACAAATG+TGG 0.593107 tig0026340:+40059 Msa1428390:CDS
CAATATTGAGTGAATTGTGT+AGG 0.597629 tig0026340:+40109 Msa1428390:exon
AGAATTAGACTCTGATGATG+AGG 0.598339 tig0026340:+38057 Msa1428390:CDS
ACTAGATTGGATCATCACTT+CGG 0.604341 tig0026340:-37380 None:intergenic
CTAGCAGAGAGGAAAGGCAG+TGG 0.608241 tig0026340:+38374 Msa1428390:intron
CAGAAGGATGGGGACTGACG+GGG 0.612887 tig0026340:+38526 Msa1428390:CDS
GAAGCTTGATTCAGACGGCA+AGG 0.613499 tig0026340:+39378 Msa1428390:CDS
TGAGTACTAGCAGAGAGGAA+AGG 0.624923 tig0026340:+38368 Msa1428390:intron
AGCTGACACGACAACAGGCG+AGG 0.627903 tig0026340:+38664 Msa1428390:CDS
GAATGAGGTGTATTGTTGTG+CGG 0.631430 tig0026340:+40172 Msa1428390:three_prime_UTR
GAAAGTCGAAACAAATGTGG+AGG 0.633362 tig0026340:+40062 Msa1428390:CDS
CTACCAGAACCAGAAGGATG+GGG 0.636130 tig0026340:+38516 Msa1428390:CDS
TACCTGATTATGATATACAA+AGG 0.642517 tig0026340:+39583 Msa1428390:CDS
ACTAGCAAAGTAACATATGG+TGG 0.655228 tig0026340:+38470 Msa1428390:CDS
ATACTTCTACCAGAACCAGA+AGG 0.666727 tig0026340:+38510 Msa1428390:CDS
GAAGACTGAGAACACAAGCA+GGG 0.671813 tig0026340:+38015 Msa1428390:CDS
AGTGATGATCCAATCTAGTG+AGG 0.673139 tig0026340:+37384 Msa1428390:five_prime_UTR
CAAATCAGAAGAACCCTCGA+AGG 0.686390 tig0026340:+38421 Msa1428390:CDS
GCAACACATAGAATCACTAG+AGG 0.698772 tig0026340:+38686 Msa1428390:CDS
TGAGTGAATTGTGTAGGACA+TGG 0.729356 tig0026340:+40115 Msa1428390:exon
TCATAGGCTGAATATCAACA+GGG 0.751976 tig0026340:+37710 Msa1428390:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTTTTTAAAAATTTA+AGG - tig0026340:37613-37632 None:intergenic 0.0%
!!! AAATTTTTAAAAAATATAAT+TGG + tig0026340:37614-37633 Msa1428390:five_prime_UTR 0.0%
!!! AATTTTTAAAAAATATAATT+GGG + tig0026340:37615-37634 Msa1428390:five_prime_UTR 0.0%
!!! ACATTTTTTTGACAATTTTA+AGG - tig0026340:37440-37459 None:intergenic 15.0%
!!! ATCTTATCATTATACTATTT+TGG + tig0026340:38210-38229 Msa1428390:intron 15.0%
!! TTGAACTTATTTATTTAGTT+TGG + tig0026340:38319-38338 Msa1428390:intron 15.0%
!! AAAAAATGTCAAATTATGTA+TGG + tig0026340:38824-38843 Msa1428390:intron 15.0%
!! TTAAATGAAGTAAACAATTT+CGG - tig0026340:39710-39729 None:intergenic 15.0%
!! AATTGTTTACTTCATTTAAA+TGG + tig0026340:39711-39730 Msa1428390:intron 15.0%
!! ATTGTTTACTTCATTTAAAT+GGG + tig0026340:39712-39731 Msa1428390:intron 15.0%
!!! TATTATATGTTTGTTTTTCT+TGG + tig0026340:40233-40252 Msa1428390:three_prime_UTR 15.0%
!! ACCTTCATAAAATAAAACTT+TGG + tig0026340:37519-37538 Msa1428390:five_prime_UTR 20.0%
!!! TCCAAAGTTTTATTTTATGA+AGG - tig0026340:37523-37542 None:intergenic 20.0%
!! TTAGTGTTTAAATACTTCAT+AGG + tig0026340:37686-37705 Msa1428390:five_prime_UTR 20.0%
!!! CAATAGAAGTTTTTTTATCA+CGG + tig0026340:38175-38194 Msa1428390:intron 20.0%
!!! TATTTAGTTTGGTTCATATA+TGG + tig0026340:38330-38349 Msa1428390:intron 20.0%
!! GTTAATATTTAAGGAAATGA+AGG + tig0026340:39145-39164 Msa1428390:intron 20.0%
!! TTAGTTAGTTGATTAGAAAA+GGG + tig0026340:39186-39205 Msa1428390:intron 20.0%
!!! GATAACAATGTCTTATTTAT+AGG - tig0026340:39250-39269 None:intergenic 20.0%
!! TTCTCTGTTATTGTATAATA+AGG + tig0026340:39278-39297 Msa1428390:intron 20.0%
!! AACAAAAAAATGATAGGAAT+CGG - tig0026340:39306-39325 None:intergenic 20.0%
!!! TCCTATCATTTTTTTGTTTT+AGG + tig0026340:39308-39327 Msa1428390:intron 20.0%
!! ACCTAAAACAAAAAAATGAT+AGG - tig0026340:39312-39331 None:intergenic 20.0%
!!! TTTTTTTGTTTTAGGTATTG+AGG + tig0026340:39316-39335 Msa1428390:intron 20.0%
!! ATTGTACGATTTCAAAATAA+TGG + tig0026340:39684-39703 Msa1428390:intron 20.0%
!! ATTGTGTTACAAATTAAGTT+AGG - tig0026340:39743-39762 None:intergenic 20.0%
!!! TATACACTATCTTCATTTTT+TGG + tig0026340:39820-39839 Msa1428390:intron 20.0%
!!! ATACACTATCTTCATTTTTT+GGG + tig0026340:39821-39840 Msa1428390:intron 20.0%
!! TAATATGAATTTCAAATCGA+GGG - tig0026340:40322-40341 None:intergenic 20.0%
! AAATAAAACTTTGGATGATG+TGG + tig0026340:37528-37547 Msa1428390:five_prime_UTR 25.0%
!!! TTCTAAGTTTTTTGACATGA+TGG + tig0026340:37742-37761 Msa1428390:five_prime_UTR 25.0%
!!! ATGGTTTGTTGTGTTTTATT+TGG + tig0026340:37761-37780 Msa1428390:five_prime_UTR 25.0%
! TTAGATAAAGAATATGCAGA+AGG + tig0026340:37808-37827 Msa1428390:exon 25.0%
! TAGATAAAGAATATGCAGAA+GGG + tig0026340:37809-37828 Msa1428390:exon 25.0%
!! ACAATGTTTTCAAATCAAGA+GGG + tig0026340:37846-37865 Msa1428390:CDS 25.0%
! CTATATGATATAACTGAATC+AGG + tig0026340:38619-38638 Msa1428390:intron 25.0%
! TGTTAAGTGTAGATGAAATA+AGG + tig0026340:38775-38794 Msa1428390:intron 25.0%
! AAAGAAAAACCTTCTACATT+AGG + tig0026340:38801-38820 Msa1428390:intron 25.0%
!!! ACATTTTTTCCTAATGTAGA+AGG - tig0026340:38813-38832 None:intergenic 25.0%
! AACCAGGTTGTTAATATTTA+AGG + tig0026340:39136-39155 Msa1428390:intron 25.0%
! TTCCTTAAATATTAACAACC+TGG - tig0026340:39141-39160 None:intergenic 25.0%
! CTTAGTTAGTTGATTAGAAA+AGG + tig0026340:39185-39204 Msa1428390:intron 25.0%
! TGATTAGAAAAGGGTTAATT+AGG + tig0026340:39195-39214 Msa1428390:intron 25.0%
! TTAAGTTTCTATATGTATGC+AGG + tig0026340:39339-39358 Msa1428390:intron 25.0%
! TAAGTTTCTATATGTATGCA+GGG + tig0026340:39340-39359 Msa1428390:intron 25.0%
! GTGGTAAAAAGAAATAATGA+TGG + tig0026340:39484-39503 Msa1428390:intron 25.0%
! TACCTGATTATGATATACAA+AGG + tig0026340:39583-39602 Msa1428390:CDS 25.0%
! TTCCTTTGTATATCATAATC+AGG - tig0026340:39588-39607 None:intergenic 25.0%
! TATGATATACAAAGGAAGAA+AGG + tig0026340:39591-39610 Msa1428390:CDS 25.0%
! ACAATAAGAAACTCAAATTG+AGG - tig0026340:39669-39688 None:intergenic 25.0%
! GATTCTACATTAAACAAACA+TGG - tig0026340:39884-39903 None:intergenic 25.0%
! AATGTAGAATCATCATTCTT+CGG + tig0026340:39893-39912 Msa1428390:intron 25.0%
! AGTATGTAAAATGTAGAATG+AGG + tig0026340:40157-40176 Msa1428390:three_prime_UTR 25.0%
! CTAATATGAATTTCAAATCG+AGG - tig0026340:40323-40342 None:intergenic 25.0%
TAAATACTTCATAGGCTCAT+AGG + tig0026340:37694-37713 Msa1428390:five_prime_UTR 30.0%
! GACAATGTTTTCAAATCAAG+AGG + tig0026340:37845-37864 Msa1428390:CDS 30.0%
! GTCTTGATGAGAAAATTTTG+AGG + tig0026340:38089-38108 Msa1428390:CDS 30.0%
!!! GAAAATTTTGAGGTCATCAA+TGG + tig0026340:38099-38118 Msa1428390:CDS 30.0%
! TTTTATCACGGTTAATCGAT+CGG + tig0026340:38187-38206 Msa1428390:intron 30.0%
GAAAAGACAAATTCCGATTA+CGG - tig0026340:38448-38467 None:intergenic 30.0%
TTGAGACAATTAACACAAAG+AGG - tig0026340:38574-38593 None:intergenic 30.0%
TATGATATAACTGAATCAGG+TGG + tig0026340:38622-38641 Msa1428390:intron 30.0%
TATCGTTACAAACCTTATCA+TGG - tig0026340:38715-38734 None:intergenic 30.0%
GATAAGGTTTGTAACGATAT+AGG + tig0026340:38716-38735 Msa1428390:intron 30.0%
! TTTTCATATGCTCTCCAAAA+CGG + tig0026340:39067-39086 Msa1428390:intron 30.0%
TCAAATAAGCTAATTCAACC+AGG + tig0026340:39120-39139 Msa1428390:intron 30.0%
TGTATATCATAATCAGGTAC+TGG - tig0026340:39582-39601 None:intergenic 30.0%
CACAAAGGGAACAAAAAAAA+AGG - tig0026340:39621-39640 None:intergenic 30.0%
AGCATCAATATTCACACAAA+GGG - tig0026340:39635-39654 None:intergenic 30.0%
AAGCATCAATATTCACACAA+AGG - tig0026340:39636-39655 None:intergenic 30.0%
TAAGAAACTCAAATTGAGGA+AGG - tig0026340:39665-39684 None:intergenic 30.0%
!! AATATTGCAGATTCTAGAGT+TGG + tig0026340:39960-39979 Msa1428390:intron 30.0%
AGCTAAACAAAAACAAGGTT+TGG + tig0026340:39985-40004 Msa1428390:CDS 30.0%
TATGAAAGTCGAAACAAATG+TGG + tig0026340:40059-40078 Msa1428390:CDS 30.0%
GAAGAACAAAGAAAGTTGTT+AGG + tig0026340:40084-40103 Msa1428390:CDS 30.0%
CAATATTGAGTGAATTGTGT+AGG + tig0026340:40109-40128 Msa1428390:exon 30.0%
ACTAGATTGGATCATCACTT+CGG - tig0026340:37383-37402 None:intergenic 35.0%
TCATAGGCTGAATATCAACA+GGG + tig0026340:37710-37729 Msa1428390:five_prime_UTR 35.0%
! GTTTTCAAATCAAGAGGGTT+TGG + tig0026340:37851-37870 Msa1428390:CDS 35.0%
!!! TTTGGAGATTTTGGTGGATT+TGG + tig0026340:37869-37888 Msa1428390:CDS 35.0%
GTGAAAAAAGGATCGTCAAA+TGG - tig0026340:37941-37960 None:intergenic 35.0%
GATCTAGATGAAGTAGAACT+TGG - tig0026340:37989-38008 None:intergenic 35.0%
! AGAATTAGACTCTGATGATG+AGG + tig0026340:38057-38076 Msa1428390:CDS 35.0%
! TTTGCTTGACAAGTTCTGAT+TGG + tig0026340:38255-38274 Msa1428390:intron 35.0%
CAGACTAGCAAAGTAACATA+TGG + tig0026340:38467-38486 Msa1428390:CDS 35.0%
ACTAGCAAAGTAACATATGG+TGG + tig0026340:38470-38489 Msa1428390:CDS 35.0%
GTAACATATGGTGGTATAGA+TGG + tig0026340:38479-38498 Msa1428390:CDS 35.0%
TATATGTTCAACAGTTGCTC+AGG + tig0026340:38861-38880 Msa1428390:intron 35.0%
TAACACACTAGTCAATCAAC+AGG - tig0026340:38891-38910 None:intergenic 35.0%
! CTAGTGTGTTAGCTTCTATT+TGG + tig0026340:38900-38919 Msa1428390:intron 35.0%
TCACTAGTGGTTATGTAAGT+AGG + tig0026340:39092-39111 Msa1428390:intron 35.0%
TTGTTTAACAACATCTCGAG+TGG + tig0026340:39465-39484 Msa1428390:intron 35.0%
!!! TTTTTGGGTGCAACTTAGAT+TGG + tig0026340:39836-39855 Msa1428390:intron 35.0%
TGGTGAGCTAAACAAAAACA+AGG + tig0026340:39980-39999 Msa1428390:CDS 35.0%
! AATCCAATCAAATCGAGTTG+AGG + tig0026340:40349-40368 Msa1428390:three_prime_UTR 35.0%
! AAACCTCAACTCGATTTGAT+TGG - tig0026340:40355-40374 None:intergenic 35.0%
! TGGTCTCTAACTTGCATATG+TGG + tig0026340:37346-37365 Msa1428390:five_prime_UTR 40.0%
AGTGATGATCCAATCTAGTG+AGG + tig0026340:37384-37403 Msa1428390:five_prime_UTR 40.0%
TTAAGGATTGTGATCCTGCT+GGG - tig0026340:37423-37442 None:intergenic 40.0%
TTTAAGGATTGTGATCCTGC+TGG - tig0026340:37424-37443 None:intergenic 40.0%
CGTCAGTTGCACTTCAAATT+CGG + tig0026340:37472-37491 Msa1428390:five_prime_UTR 40.0%
CTCATAGGCTGAATATCAAC+AGG + tig0026340:37709-37728 Msa1428390:five_prime_UTR 40.0%
! TCAAGAGGGTTTGGAGATTT+TGG + tig0026340:37860-37879 Msa1428390:CDS 40.0%
!! TAGGCATCGTCATACTTTTG+TGG - tig0026340:37898-37917 None:intergenic 40.0%
!! ATGACGATGCCTAGTCTTTT+TGG + tig0026340:37905-37924 Msa1428390:CDS 40.0%
TCTCTTCCTCCAAAAAGACT+AGG - tig0026340:37917-37936 None:intergenic 40.0%
TCAAACGGGTGAGTGAAAAA+AGG - tig0026340:37953-37972 None:intergenic 40.0%
CCATTAACATCATCATCGTC+TGG - tig0026340:38142-38161 None:intergenic 40.0%
! CCAGACGATGATGATGTTAA+TGG + tig0026340:38139-38158 Msa1428390:CDS 40.0%
ATACTTCTACCAGAACCAGA+AGG + tig0026340:38510-38529 Msa1428390:CDS 40.0%
GCAACACATAGAATCACTAG+AGG + tig0026340:38686-38705 Msa1428390:CDS 40.0%
CACTAGAGGAATCCATGATA+AGG + tig0026340:38700-38719 Msa1428390:CDS 40.0%
AAGCTATCGTAGAGAGCTTA+TGG + tig0026340:39029-39048 Msa1428390:intron 40.0%
GGAAATGAAGGATCTGTGAT+CGG + tig0026340:39157-39176 Msa1428390:intron 40.0%
TTGAGGAAGCTTGATTCAGA+CGG + tig0026340:39373-39392 Msa1428390:CDS 40.0%
ACGCTGCACAATCTTAATGA+AGG + tig0026340:39415-39434 Msa1428390:CDS 40.0%
AGATGAGCTTACACACTTTG+AGG + tig0026340:39530-39549 Msa1428390:intron 40.0%
! TTACACACTTTGAGGAAGCT+TGG + tig0026340:39538-39557 Msa1428390:CDS 40.0%
!! CTTGGAAAGGCACTAATCTA+GGG + tig0026340:39556-39575 Msa1428390:CDS 40.0%
! GGTTTGGTCACTTTCATCTT+TGG + tig0026340:40001-40020 Msa1428390:CDS 40.0%
GAGCAAGATAGAAGAGAAAG+AGG + tig0026340:40023-40042 Msa1428390:CDS 40.0%
GAAAGTCGAAACAAATGTGG+AGG + tig0026340:40062-40081 Msa1428390:CDS 40.0%
TGAGTGAATTGTGTAGGACA+TGG + tig0026340:40115-40134 Msa1428390:exon 40.0%
GAATGAGGTGTATTGTTGTG+CGG + tig0026340:40172-40191 Msa1428390:three_prime_UTR 40.0%
! AGAGGGTTTGGAGATTTTGG+TGG + tig0026340:37863-37882 Msa1428390:CDS 45.0%
!! ACGATGCCTAGTCTTTTTGG+AGG + tig0026340:37908-37927 Msa1428390:CDS 45.0%
GATTCCTCAAGCTGTCAAAC+GGG - tig0026340:37967-37986 None:intergenic 45.0%
GGATTCCTCAAGCTGTCAAA+CGG - tig0026340:37968-37987 None:intergenic 45.0%
AGAAGACTGAGAACACAAGC+AGG + tig0026340:38014-38033 Msa1428390:CDS 45.0%
GAAGACTGAGAACACAAGCA+GGG + tig0026340:38015-38034 Msa1428390:CDS 45.0%
GAGAACACAAGCAGGGAAAA+AGG + tig0026340:38022-38041 Msa1428390:CDS 45.0%
TGAGTACTAGCAGAGAGGAA+AGG + tig0026340:38368-38387 Msa1428390:intron 45.0%
CAAATCAGAAGAACCCTCGA+AGG + tig0026340:38421-38440 Msa1428390:CDS 45.0%
TTCTACCAGAACCAGAAGGA+TGG + tig0026340:38514-38533 Msa1428390:CDS 45.0%
TCTACCAGAACCAGAAGGAT+GGG + tig0026340:38515-38534 Msa1428390:CDS 45.0%
TAACACAAAGAGGAAGTCGC+AGG - tig0026340:38564-38583 None:intergenic 45.0%
AAAGAAGCTGACACGACAAC+AGG + tig0026340:38659-38678 Msa1428390:CDS 45.0%
! AACCACTAGTGAGACCGTTT+TGG - tig0026340:39084-39103 None:intergenic 45.0%
! TGCAGGGTCATTCAGTTTTG+AGG + tig0026340:39356-39375 Msa1428390:intron 45.0%
! CACTTTGAGGAAGCTTGGAA+AGG + tig0026340:39543-39562 Msa1428390:CDS 45.0%
!! GCTTGGAAAGGCACTAATCT+AGG + tig0026340:39555-39574 Msa1428390:CDS 45.0%
CACAAGCAGGGAAAAAGGAG+TGG + tig0026340:38027-38046 Msa1428390:CDS 50.0%
TCGTCTGGATGCTCAACAGA+TGG - tig0026340:38127-38146 None:intergenic 50.0%
TCGTGTGAGTACTAGCAGAG+AGG + tig0026340:38363-38382 Msa1428390:intron 50.0%
AACCCTCGAAGGACCGTAAT+CGG + tig0026340:38432-38451 Msa1428390:CDS 50.0%
TTCCGATTACGGTCCTTCGA+GGG - tig0026340:38437-38456 None:intergenic 50.0%
ATTCCGATTACGGTCCTTCG+AGG - tig0026340:38438-38457 None:intergenic 50.0%
CTACCAGAACCAGAAGGATG+GGG + tig0026340:38516-38535 Msa1428390:CDS 50.0%
ACTGAATCAGGTGGCGATTG+AGG + tig0026340:38631-38650 Msa1428390:intron 50.0%
CCACTGCAGAAATGCGTTCT+TGG - tig0026340:38743-38762 None:intergenic 50.0%
CCAAGAACGCATTTCTGCAG+TGG + tig0026340:38740-38759 Msa1428390:intron 50.0%
CTCCAAAACGGTCTCACTAG+TGG + tig0026340:39079-39098 Msa1428390:intron 50.0%
GAAGCTTGATTCAGACGGCA+AGG + tig0026340:39378-39397 Msa1428390:CDS 50.0%
CTTGATTCAGACGGCAAGGT+TGG + tig0026340:39382-39401 Msa1428390:CDS 50.0%
CTCACCCGTTTGACAGCTTG+AGG + tig0026340:37960-37979 Msa1428390:CDS 55.0%
CTAGCAGAGAGGAAAGGCAG+TGG + tig0026340:38374-38393 Msa1428390:intron 55.0%
!! AGTCCCCATCCTTCTGGTTC+TGG - tig0026340:38522-38541 None:intergenic 55.0%
ACCAGAAGGATGGGGACTGA+CGG + tig0026340:38524-38543 Msa1428390:CDS 55.0%
GAGGGGGAGCCTCACTAGAT+TGG - tig0026340:37396-37415 None:intergenic 60.0%
TCTAGTGAGGCTCCCCCTCA+TGG + tig0026340:37397-37416 Msa1428390:five_prime_UTR 60.0%
TGATCCTGCTGGGCCATGAG+GGG - tig0026340:37413-37432 None:intergenic 60.0%
GTGATCCTGCTGGGCCATGA+GGG - tig0026340:37414-37433 None:intergenic 60.0%
TGTGATCCTGCTGGGCCATG+AGG - tig0026340:37415-37434 None:intergenic 60.0%
CCAGAAGGATGGGGACTGAC+GGG + tig0026340:38525-38544 Msa1428390:CDS 60.0%
CAGAAGGATGGGGACTGACG+GGG + tig0026340:38526-38545 Msa1428390:CDS 60.0%
AGCTGACACGACAACAGGCG+AGG + tig0026340:38664-38683 Msa1428390:CDS 60.0%
GATCCTGCTGGGCCATGAGG+GGG - tig0026340:37412-37431 None:intergenic 65.0%
CCCGTCAGTCCCCATCCTTC+TGG - tig0026340:38528-38547 None:intergenic 65.0%
GCTCCCCCTCATGGCCCAGC+AGG + tig0026340:37406-37425 Msa1428390:five_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
tig0026340 gene 37329 40380 37329 ID=Msa1428390;Name=Msa1428390
tig0026340 mRNA 37329 40380 37329 ID=Msa1428390-mRNA-1;Parent=Msa1428390;Name=Msa1428390-mRNA-1;_AED=0.11;_eAED=0.11;_QI=492|1|1|1|1|1|6|259|298
tig0026340 exon 37329 38160 37329 ID=Msa1428390-mRNA-1:exon:5933;Parent=Msa1428390-mRNA-1
tig0026340 exon 38381 38547 38381 ID=Msa1428390-mRNA-1:exon:5934;Parent=Msa1428390-mRNA-1
tig0026340 exon 38641 38721 38641 ID=Msa1428390-mRNA-1:exon:5935;Parent=Msa1428390-mRNA-1
tig0026340 exon 39361 39436 39361 ID=Msa1428390-mRNA-1:exon:5936;Parent=Msa1428390-mRNA-1
tig0026340 exon 39532 39612 39532 ID=Msa1428390-mRNA-1:exon:5937;Parent=Msa1428390-mRNA-1
tig0026340 exon 39970 40380 39970 ID=Msa1428390-mRNA-1:exon:5938;Parent=Msa1428390-mRNA-1
tig0026340 five_prime_UTR 37329 37820 37329 ID=Msa1428390-mRNA-1:five_prime_utr;Parent=Msa1428390-mRNA-1
tig0026340 CDS 37821 38160 37821 ID=Msa1428390-mRNA-1:cds;Parent=Msa1428390-mRNA-1
tig0026340 CDS 38381 38547 38381 ID=Msa1428390-mRNA-1:cds;Parent=Msa1428390-mRNA-1
tig0026340 CDS 38641 38721 38641 ID=Msa1428390-mRNA-1:cds;Parent=Msa1428390-mRNA-1
tig0026340 CDS 39361 39436 39361 ID=Msa1428390-mRNA-1:cds;Parent=Msa1428390-mRNA-1
tig0026340 CDS 39532 39612 39532 ID=Msa1428390-mRNA-1:cds;Parent=Msa1428390-mRNA-1
tig0026340 CDS 39970 40121 39970 ID=Msa1428390-mRNA-1:cds;Parent=Msa1428390-mRNA-1
tig0026340 three_prime_UTR 40122 40380 40122 ID=Msa1428390-mRNA-1:three_prime_utr;Parent=Msa1428390-mRNA-1
Gene Sequence

>Msa1428390

ATGCAGAAGGGTAGAGAAGTTGAAGACAATGTTTTCAAATCAAGAGGGTTTGGAGATTTTGGTGGATTTGGCTTCCACAAAAGTATGACGATGCCTAGTCTTTTTGGAGGAAGAGATCCATTTGACGATCCTTTTTTCACTCACCCGTTTGACAGCTTGAGGAATCCAAGTTCTACTTCATCTAGATCAATGCAGAAGACTGAGAACACAAGCAGGGAAAAAGGAGTGGTTATAAAAGAATTAGACTCTGATGATGAGGACAATGATAGTCTTGATGAGAAAATTTTGAGGTCATCAATGGAGCCATCTGTTGAGCATCCAGACGATGATGATGTTAATGAGAGGAAAGGCAGTGGCATGACTCACAAGATTGATCTTCACAAATCAGAAGAACCCTCGAAGGACCGTAATCGGAATTTGTCTTTTCAGACTAGCAAAGTAACATATGGTGGTATAGATGGTGCTTACTATACTTCTACCAGAACCAGAAGGATGGGGACTGACGGGGTGGCGATTGAGGAAAGCAAAGAAGCTGACACGACAACAGGCGAGGCAACACATAGAATCACTAGAGGAATCCATGATAAGGGTCATTCAGTTTTGAGGAAGCTTGATTCAGACGGCAAGGTTGGTACAACACAAACGCTGCACAATCTTAATGAAGATGAGCTTACACACTTTGAGGAAGCTTGGAAAGGCACTAATCTAGGGCCAGTACCTGATTATGATATACAAAGGAAGAAAGATTCTAGAGTTGGTGAGCTAAACAAAAACAAGGTTTGGTCACTTTCATCTTTGGAGCAAGATAGAAGAGAAAGAGGATTTTCATCAAATTATGAAAGTCGAAACAAATGTGGAGGAAGAACAAAGAAAGTTGTTAGGATCAATATTGAGTGA
Protein sequence

>Msa1428390

MQKGREVEDNVFKSRGFGDFGGFGFHKSMTMPSLFGGRDPFDDPFFTHPFDSLRNPSSTSSRSMQKTENTSREKGVVIKELDSDDEDNDSLDEKILRSSMEPSVEHPDDDDVNERKGSGMTHKIDLHKSEEPSKDRNRNLSFQTSKVTYGGIDGAYYTSTRTRRMGTDGVAIEESKEADTTTGEATHRITRGIHDKGHSVLRKLDSDGKVGTTQTLHNLNEDELTHFEEAWKGTNLGPVPDYDIQRKKDSRVGELNKNKVWSLSSLEQDRRERGFSSNYESRNKCGGRTKKVVRINIE*