AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1211010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1211010 MtrunA17_Chr8g0337261 84.615 169 7 2 1 167 1 152 2.40e-56 174
Msa1211010 MtrunA17_Chr8g0337251 62.275 167 27 7 1 167 1 131 6.67e-31 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 75 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCCCTCAACTTTATTT+AGG 0.238868 8_1:-70385146 Msa1211010:three_prime_UTR
AAGTTTAAGCGTGGAAAATT+TGG 0.266461 8_1:-70385375 Msa1211010:CDS
CAACTTTATTTAGGTCTGTT+TGG 0.271608 8_1:-70385137 Msa1211010:three_prime_UTR
ATGAACAATTATTGTTCATT+TGG 0.293223 8_1:-70385214 Msa1211010:three_prime_UTR
AAGTATAAGCATGGAAAATT+TGG 0.307873 8_1:-70385432 Msa1211010:CDS
TATCATCATGGTTATGGTTA+TGG 0.313342 8_1:-70385462 Msa1211010:CDS
AAGCATGGTAGGTTTGGTTT+TGG 0.332336 8_1:-70385399 Msa1211010:CDS
TCTTATCATGCTCCACAATA+TGG 0.348923 8_1:-70386100 Msa1211010:CDS
TATGGCTATCCATATCCTTC+TGG 0.365272 8_1:-70386082 Msa1211010:intron
GGAGGTTATCATCATGGTTA+TGG 0.376411 8_1:-70385468 Msa1211010:CDS
CGTTGGAAGCATGGTAGGTT+TGG 0.389621 8_1:-70385405 Msa1211010:CDS
TCAAGTGTACCAGAAGGATA+TGG 0.413672 8_1:+70386073 None:intergenic
CCTTCCGGTTATCCTCATTC+CGG 0.421172 8_1:-70386139 Msa1211010:CDS
GAACCAGGTGGAGGAGGATA+AGG 0.429948 8_1:+70386271 None:intergenic
GGAGGATAAGGTGGTGCTGC+TGG 0.432218 8_1:+70386283 None:intergenic
TGTTATAAAGGGCGTGGATC+TGG 0.441402 8_1:-70385555 Msa1211010:intron
ATATCCGGAATGAGGATAAC+CGG 0.442176 8_1:+70386135 None:intergenic
GGTATAGGAGGATTAATTGC+AGG 0.442489 8_1:-70385534 Msa1211010:CDS
GCAAGCGATGGAAGTGATGC+AGG 0.445817 8_1:-70385353 Msa1211010:exon
GGAGGAGGGTATCCACCTTC+CGG 0.452585 8_1:-70386154 Msa1211010:CDS
GCAGGGGCGGCAGCTGCATA+CGG 0.453407 8_1:-70385516 Msa1211010:CDS
AAAGGGCGTGGATCTGGTAT+AGG 0.462773 8_1:-70385549 Msa1211010:intron
CACCATCTGTCGCATGGACA+TGG 0.469597 8_1:-70385489 Msa1211010:CDS
CATGGTTATGGTTATGGACA+TGG 0.469853 8_1:-70385456 Msa1211010:CDS
CCACCACATGCTGCAGGAGG+AGG 0.490677 8_1:-70386169 Msa1211010:CDS
AGATGGTTGATGATGATATC+CGG 0.500001 8_1:+70386120 None:intergenic
TTTGGCAAGCGTTGGAAGCA+TGG 0.515401 8_1:-70385414 Msa1211010:CDS
ACAGACCTAAATAAAGTTGA+GGG 0.520171 8_1:+70385141 None:intergenic
ATGGAAAATTTGGCAAGCGT+TGG 0.520922 8_1:-70385422 Msa1211010:CDS
CCACCTTATCCTCCTCCACC+TGG 0.523771 8_1:-70386274 Msa1211010:CDS
GAATAAGAACCAGGTGGAGG+AGG 0.530977 8_1:+70386265 None:intergenic
CTCCATGTCCATGCGACAGA+TGG 0.531469 8_1:+70385487 None:intergenic
TATACCTCCTTGAGGTGGAT+AGG 0.536217 8_1:+70386243 None:intergenic
GCAAGCGTTGGAAGCATGGT+AGG 0.542067 8_1:-70385410 Msa1211010:CDS
AACAGACCTAAATAAAGTTG+AGG 0.548568 8_1:+70385140 None:intergenic
CAATATCCACCACATGCTGC+AGG 0.549438 8_1:-70386175 Msa1211010:CDS
CACCACATGCTGCAGGAGGA+GGG 0.555583 8_1:-70386168 Msa1211010:CDS
GGACATGGAGGTTATCATCA+TGG 0.565727 8_1:-70385474 Msa1211010:CDS
GGATATGGATAGCCATATTG+TGG 0.568164 8_1:+70386088 None:intergenic
CAAGCGATGGAAGTGATGCA+GGG 0.575898 8_1:-70385352 Msa1211010:exon
TACCCTCCTCCTGCAGCATG+TGG 0.578490 8_1:+70386166 None:intergenic
CCTCCTCCTGCAGCATGTGG+TGG 0.578595 8_1:+70386169 None:intergenic
TCTTATTCCTATCCACCTCA+AGG 0.583936 8_1:-70386250 Msa1211010:CDS
GTATAGGAGGATTAATTGCA+GGG 0.585893 8_1:-70385533 Msa1211010:CDS
GGGCGTGGATCTGGTATAGG+AGG 0.586096 8_1:-70385546 Msa1211010:CDS
TATTGTGGAGCATGATAAGA+TGG 0.586561 8_1:+70386103 None:intergenic
GGATAATAACCTCCTTGAGG+TGG 0.602130 8_1:+70386202 None:intergenic
GTGGAAAATTTGGCAAGCGA+TGG 0.603805 8_1:-70385365 Msa1211010:CDS
CCAGGTGGAGGAGGATAAGG+TGG 0.606697 8_1:+70386274 None:intergenic
GGACATGGGAAGTATAAGCA+TGG 0.607097 8_1:-70385441 Msa1211010:CDS
GGTGCTCACCATCTGTCGCA+TGG 0.609238 8_1:-70385495 Msa1211010:CDS
TAGGAATAAGAACCAGGTGG+AGG 0.612421 8_1:+70386262 None:intergenic
GATCAAGAATCTAGTGAGAG+AGG 0.615149 8_1:-70386331 Msa1211010:CDS
AGGAGGATTAATTGCAGGGG+CGG 0.617439 8_1:-70385529 Msa1211010:CDS
ATGGTTATGGTTATGGACAT+GGG 0.618834 8_1:-70385455 Msa1211010:CDS
GAATGAGGATAACCGGAAGG+TGG 0.623841 8_1:+70386142 None:intergenic
GGTGGATAGGAATAAGAACC+AGG 0.628633 8_1:+70386256 None:intergenic
AAGCGATGGAAGTGATGCAG+GGG 0.637098 8_1:-70385351 Msa1211010:exon
TGATGATGATATCCGGAATG+AGG 0.639527 8_1:+70386127 None:intergenic
GTGGATATACCTCCTTGAGG+TGG 0.648572 8_1:+70386238 None:intergenic
AGGTGGATATGCCGTTGCGG+TGG 0.649244 8_1:+70386219 None:intergenic
AGGAGGTATATCCACCGCAA+CGG 0.658067 8_1:-70386230 Msa1211010:CDS
GGGTGAAACAACATGAACAA+TGG 0.660870 8_1:-70386367 None:intergenic
GCAACGGCATATCCACCTCA+AGG 0.661556 8_1:-70386214 Msa1211010:CDS
TATCCACCACATGCTGCAGG+AGG 0.666317 8_1:-70386172 Msa1211010:CDS
CCGGAATGAGGATAACCGGA+AGG 0.667322 8_1:+70386139 None:intergenic
GCATGTGGTGGATATTGTGA+AGG 0.678658 8_1:+70386181 None:intergenic
CATCTGTCGCATGGACATGG+AGG 0.679502 8_1:-70385486 Msa1211010:CDS
GAAGGATAATAACCTCCTTG+AGG 0.682876 8_1:+70386199 None:intergenic
TTGAGGTGGATATGCCGTTG+CGG 0.703511 8_1:+70386216 None:intergenic
TATTCCTATCCACCTCAAGG+AGG 0.707945 8_1:-70386247 Msa1211010:CDS
GGATAGGAATAAGAACCAGG+TGG 0.722057 8_1:+70386259 None:intergenic
GCGGTGGATATACCTCCTTG+AGG 0.738109 8_1:+70386235 None:intergenic
TATAGGAGGATTAATTGCAG+GGG 0.742815 8_1:-70385532 Msa1211010:CDS
ACGGCATATCCACCTCAAGG+AGG 0.749279 8_1:-70386211 Msa1211010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTATTGTTGTTATAAA+GGG - chr8_1:70385896-70385915 Msa1211010:intron 10.0%
!!! TAATTTATTGTTGTTATAAA+GGG - chr8_1:70385896-70385915 Msa1211010:intron 10.0%
!! AATATACTCCATAAAAAATT+GGG + chr8_1:70385419-70385438 None:intergenic 15.0%
!! TAATATACTCCATAAAAAAT+TGG + chr8_1:70385420-70385439 None:intergenic 15.0%
!!! TACTAGTTTCAAATATTATT+AGG - chr8_1:70385466-70385485 Msa1211010:CDS 15.0%
!!! CTAATTTATTGTTGTTATAA+AGG - chr8_1:70385895-70385914 Msa1211010:intron 15.0%
!! AATATACTCCATAAAAAATT+GGG + chr8_1:70385419-70385438 None:intergenic 15.0%
!! TAATATACTCCATAAAAAAT+TGG + chr8_1:70385420-70385439 None:intergenic 15.0%
!!! TACTAGTTTCAAATATTATT+AGG - chr8_1:70385466-70385485 Msa1211010:CDS 15.0%
!!! CTAATTTATTGTTGTTATAA+AGG - chr8_1:70385895-70385914 Msa1211010:intron 15.0%
!!! ACATTTTCTGCTTTTTTTAT+TGG - chr8_1:70385507-70385526 Msa1211010:CDS 20.0%
!!! CATTTTCTGCTTTTTTTATT+GGG - chr8_1:70385508-70385527 Msa1211010:CDS 20.0%
!! ATGAACAATTATTGTTCATT+TGG - chr8_1:70386248-70386267 Msa1211010:CDS 20.0%
!!! ACATTTTCTGCTTTTTTTAT+TGG - chr8_1:70385507-70385526 Msa1211010:CDS 20.0%
!!! CATTTTCTGCTTTTTTTATT+GGG - chr8_1:70385508-70385527 Msa1211010:CDS 20.0%
!! ATGAACAATTATTGTTCATT+TGG - chr8_1:70386248-70386267 Msa1211010:CDS 20.0%
!!! TTGATCATCCCAATTTTTTA+TGG - chr8_1:70385408-70385427 Msa1211010:CDS 25.0%
!!! TATAAAGTGTTGTCAAATAG+AGG - chr8_1:70385601-70385620 Msa1211010:intron 25.0%
! AACAACATTGATCACAAAAA+TGG - chr8_1:70385692-70385711 Msa1211010:intron 25.0%
!!! AGATCAATGTTTTTTGCTAA+TGG - chr8_1:70385734-70385753 Msa1211010:intron 25.0%
! AAGTATAAGCATGGAAAATT+TGG - chr8_1:70386030-70386049 Msa1211010:intron 25.0%
!!! TTGATCATCCCAATTTTTTA+TGG - chr8_1:70385408-70385427 Msa1211010:CDS 25.0%
!!! TATAAAGTGTTGTCAAATAG+AGG - chr8_1:70385601-70385620 Msa1211010:intron 25.0%
! AACAACATTGATCACAAAAA+TGG - chr8_1:70385692-70385711 Msa1211010:intron 25.0%
!!! AGATCAATGTTTTTTGCTAA+TGG - chr8_1:70385734-70385753 Msa1211010:intron 25.0%
! AAGTATAAGCATGGAAAATT+TGG - chr8_1:70386030-70386049 Msa1211010:intron 25.0%
CATCTATTACAGAACAATAG+CGG + chr8_1:70385575-70385594 None:intergenic 30.0%
CAGAATTTGTACAAACTATG+TGG - chr8_1:70385655-70385674 Msa1211010:intron 30.0%
TAACACATGCAAGAAACATT+AGG + chr8_1:70385770-70385789 None:intergenic 30.0%
GTAAAACAAGACGTAATATC+AGG + chr8_1:70385812-70385831 None:intergenic 30.0%
TATCATCATGGTTATGGTTA+TGG - chr8_1:70386000-70386019 Msa1211010:intron 30.0%
AAGTTTAAGCGTGGAAAATT+TGG - chr8_1:70386087-70386106 Msa1211010:CDS 30.0%
!!! AGCAAGTTTTGAATTTCAGT+TGG - chr8_1:70386216-70386235 Msa1211010:CDS 30.0%
ACAGACCTAAATAAAGTTGA+GGG + chr8_1:70386324-70386343 None:intergenic 30.0%
AACAGACCTAAATAAAGTTG+AGG + chr8_1:70386325-70386344 None:intergenic 30.0%
! CAACTTTATTTAGGTCTGTT+TGG - chr8_1:70386325-70386344 Msa1211010:CDS 30.0%
CATCTATTACAGAACAATAG+CGG + chr8_1:70385575-70385594 None:intergenic 30.0%
CAGAATTTGTACAAACTATG+TGG - chr8_1:70385655-70385674 Msa1211010:intron 30.0%
TAACACATGCAAGAAACATT+AGG + chr8_1:70385770-70385789 None:intergenic 30.0%
GTAAAACAAGACGTAATATC+AGG + chr8_1:70385812-70385831 None:intergenic 30.0%
TATCATCATGGTTATGGTTA+TGG - chr8_1:70386000-70386019 Msa1211010:intron 30.0%
AAGTTTAAGCGTGGAAAATT+TGG - chr8_1:70386087-70386106 Msa1211010:CDS 30.0%
!!! AGCAAGTTTTGAATTTCAGT+TGG - chr8_1:70386216-70386235 Msa1211010:CDS 30.0%
ACAGACCTAAATAAAGTTGA+GGG + chr8_1:70386324-70386343 None:intergenic 30.0%
AACAGACCTAAATAAAGTTG+AGG + chr8_1:70386325-70386344 None:intergenic 30.0%
! CAACTTTATTTAGGTCTGTT+TGG - chr8_1:70386325-70386344 Msa1211010:CDS 30.0%
! GGTCTGTTTTCACATATAAC+AGG - chr8_1:70385152-70385171 Msa1211010:three_prime_UTR 35.0%
!! AGATGGTTGATGATGATATC+CGG + chr8_1:70385345-70385364 None:intergenic 35.0%
TATTGTGGAGCATGATAAGA+TGG + chr8_1:70385362-70385381 None:intergenic 35.0%
TCTTATCATGCTCCACAATA+TGG - chr8_1:70385362-70385381 Msa1211010:CDS 35.0%
ACAAGACGTAATATCAGGTA+TGG + chr8_1:70385807-70385826 None:intergenic 35.0%
! TATTGTTGTTATAAAGGGCG+TGG - chr8_1:70385901-70385920 Msa1211010:intron 35.0%
GTATAGGAGGATTAATTGCA+GGG - chr8_1:70385929-70385948 Msa1211010:intron 35.0%
TATAGGAGGATTAATTGCAG+GGG - chr8_1:70385930-70385949 Msa1211010:intron 35.0%
ATGGTTATGGTTATGGACAT+GGG - chr8_1:70386007-70386026 Msa1211010:intron 35.0%
! GTCTTTTGTTCAAATGTGTG+TGG - chr8_1:70386278-70386297 Msa1211010:CDS 35.0%
! GAAAGCCCTCAACTTTATTT+AGG - chr8_1:70386316-70386335 Msa1211010:CDS 35.0%
! GGTCTGTTTTCACATATAAC+AGG - chr8_1:70385152-70385171 Msa1211010:three_prime_UTR 35.0%
!! AGATGGTTGATGATGATATC+CGG + chr8_1:70385345-70385364 None:intergenic 35.0%
TATTGTGGAGCATGATAAGA+TGG + chr8_1:70385362-70385381 None:intergenic 35.0%
TCTTATCATGCTCCACAATA+TGG - chr8_1:70385362-70385381 Msa1211010:CDS 35.0%
ACAAGACGTAATATCAGGTA+TGG + chr8_1:70385807-70385826 None:intergenic 35.0%
! TATTGTTGTTATAAAGGGCG+TGG - chr8_1:70385901-70385920 Msa1211010:intron 35.0%
GTATAGGAGGATTAATTGCA+GGG - chr8_1:70385929-70385948 Msa1211010:intron 35.0%
TATAGGAGGATTAATTGCAG+GGG - chr8_1:70385930-70385949 Msa1211010:intron 35.0%
ATGGTTATGGTTATGGACAT+GGG - chr8_1:70386007-70386026 Msa1211010:intron 35.0%
! GTCTTTTGTTCAAATGTGTG+TGG - chr8_1:70386278-70386297 Msa1211010:CDS 35.0%
! GAAAGCCCTCAACTTTATTT+AGG - chr8_1:70386316-70386335 Msa1211010:CDS 35.0%
GATCAAGAATCTAGTGAGAG+AGG - chr8_1:70385131-70385150 Msa1211010:three_prime_UTR 40.0%
TCTTATTCCTATCCACCTCA+AGG - chr8_1:70385212-70385231 Msa1211010:three_prime_UTR 40.0%
GAAGGATAATAACCTCCTTG+AGG + chr8_1:70385266-70385285 None:intergenic 40.0%
ATATCCGGAATGAGGATAAC+CGG + chr8_1:70385330-70385349 None:intergenic 40.0%
! TGATGATGATATCCGGAATG+AGG + chr8_1:70385338-70385357 None:intergenic 40.0%
GGATATGGATAGCCATATTG+TGG + chr8_1:70385377-70385396 None:intergenic 40.0%
TATGGCTATCCATATCCTTC+TGG - chr8_1:70385380-70385399 Msa1211010:CDS 40.0%
TCAAGTGTACCAGAAGGATA+TGG + chr8_1:70385392-70385411 None:intergenic 40.0%
GCGGTTGTTAAAATTCTGCT+AGG + chr8_1:70385556-70385575 None:intergenic 40.0%
GGTATAGGAGGATTAATTGC+AGG - chr8_1:70385928-70385947 Msa1211010:intron 40.0%
GGAGGTTATCATCATGGTTA+TGG - chr8_1:70385994-70386013 Msa1211010:intron 40.0%
CATGGTTATGGTTATGGACA+TGG - chr8_1:70386006-70386025 Msa1211010:intron 40.0%
ATGGAAAATTTGGCAAGCGT+TGG - chr8_1:70386040-70386059 Msa1211010:intron 40.0%
!! AAGCATGGTAGGTTTGGTTT+TGG - chr8_1:70386063-70386082 Msa1211010:intron 40.0%
!!! GGTTTTGGAAAGTTTAAGCG+TGG - chr8_1:70386078-70386097 Msa1211010:intron 40.0%
GATCAAGAATCTAGTGAGAG+AGG - chr8_1:70385131-70385150 Msa1211010:three_prime_UTR 40.0%
TCTTATTCCTATCCACCTCA+AGG - chr8_1:70385212-70385231 Msa1211010:three_prime_UTR 40.0%
GAAGGATAATAACCTCCTTG+AGG + chr8_1:70385266-70385285 None:intergenic 40.0%
ATATCCGGAATGAGGATAAC+CGG + chr8_1:70385330-70385349 None:intergenic 40.0%
! TGATGATGATATCCGGAATG+AGG + chr8_1:70385338-70385357 None:intergenic 40.0%
GGATATGGATAGCCATATTG+TGG + chr8_1:70385377-70385396 None:intergenic 40.0%
TATGGCTATCCATATCCTTC+TGG - chr8_1:70385380-70385399 Msa1211010:CDS 40.0%
TCAAGTGTACCAGAAGGATA+TGG + chr8_1:70385392-70385411 None:intergenic 40.0%
GCGGTTGTTAAAATTCTGCT+AGG + chr8_1:70385556-70385575 None:intergenic 40.0%
GGTATAGGAGGATTAATTGC+AGG - chr8_1:70385928-70385947 Msa1211010:intron 40.0%
GGAGGTTATCATCATGGTTA+TGG - chr8_1:70385994-70386013 Msa1211010:intron 40.0%
CATGGTTATGGTTATGGACA+TGG - chr8_1:70386006-70386025 Msa1211010:intron 40.0%
ATGGAAAATTTGGCAAGCGT+TGG - chr8_1:70386040-70386059 Msa1211010:intron 40.0%
!! AAGCATGGTAGGTTTGGTTT+TGG - chr8_1:70386063-70386082 Msa1211010:intron 40.0%
!!! GGTTTTGGAAAGTTTAAGCG+TGG - chr8_1:70386078-70386097 Msa1211010:intron 40.0%
TAGGAATAAGAACCAGGTGG+AGG + chr8_1:70385203-70385222 None:intergenic 45.0%
GGATAGGAATAAGAACCAGG+TGG + chr8_1:70385206-70385225 None:intergenic 45.0%
GGTGGATAGGAATAAGAACC+AGG + chr8_1:70385209-70385228 None:intergenic 45.0%
TATTCCTATCCACCTCAAGG+AGG - chr8_1:70385215-70385234 Msa1211010:three_prime_UTR 45.0%
TATACCTCCTTGAGGTGGAT+AGG + chr8_1:70385222-70385241 None:intergenic 45.0%
GGATAATAACCTCCTTGAGG+TGG + chr8_1:70385263-70385282 None:intergenic 45.0%
!! GCATGTGGTGGATATTGTGA+AGG + chr8_1:70385284-70385303 None:intergenic 45.0%
GGATGATCAAGTGTACCAGA+AGG + chr8_1:70385398-70385417 None:intergenic 45.0%
CAGAATGATGCTCGTTTGAG+AGG - chr8_1:70385854-70385873 Msa1211010:intron 45.0%
! TGTTATAAAGGGCGTGGATC+TGG - chr8_1:70385907-70385926 Msa1211010:intron 45.0%
GGACATGGAGGTTATCATCA+TGG - chr8_1:70385988-70386007 Msa1211010:intron 45.0%
! GGACATGGGAAGTATAAGCA+TGG - chr8_1:70386021-70386040 Msa1211010:intron 45.0%
GTGGAAAATTTGGCAAGCGA+TGG - chr8_1:70386097-70386116 Msa1211010:CDS 45.0%
TAGGAATAAGAACCAGGTGG+AGG + chr8_1:70385203-70385222 None:intergenic 45.0%
GGATAGGAATAAGAACCAGG+TGG + chr8_1:70385206-70385225 None:intergenic 45.0%
GGTGGATAGGAATAAGAACC+AGG + chr8_1:70385209-70385228 None:intergenic 45.0%
TATTCCTATCCACCTCAAGG+AGG - chr8_1:70385215-70385234 Msa1211010:three_prime_UTR 45.0%
TATACCTCCTTGAGGTGGAT+AGG + chr8_1:70385222-70385241 None:intergenic 45.0%
GGATAATAACCTCCTTGAGG+TGG + chr8_1:70385263-70385282 None:intergenic 45.0%
!! GCATGTGGTGGATATTGTGA+AGG + chr8_1:70385284-70385303 None:intergenic 45.0%
GGATGATCAAGTGTACCAGA+AGG + chr8_1:70385398-70385417 None:intergenic 45.0%
CAGAATGATGCTCGTTTGAG+AGG - chr8_1:70385854-70385873 Msa1211010:intron 45.0%
! TGTTATAAAGGGCGTGGATC+TGG - chr8_1:70385907-70385926 Msa1211010:intron 45.0%
GGACATGGAGGTTATCATCA+TGG - chr8_1:70385988-70386007 Msa1211010:intron 45.0%
! GGACATGGGAAGTATAAGCA+TGG - chr8_1:70386021-70386040 Msa1211010:intron 45.0%
GTGGAAAATTTGGCAAGCGA+TGG - chr8_1:70386097-70386116 Msa1211010:CDS 45.0%
GAATAAGAACCAGGTGGAGG+AGG + chr8_1:70385200-70385219 None:intergenic 50.0%
GTGGATATACCTCCTTGAGG+TGG + chr8_1:70385227-70385246 None:intergenic 50.0%
! AGGAGGTATATCCACCGCAA+CGG - chr8_1:70385232-70385251 Msa1211010:three_prime_UTR 50.0%
TTGAGGTGGATATGCCGTTG+CGG + chr8_1:70385249-70385268 None:intergenic 50.0%
CAATATCCACCACATGCTGC+AGG - chr8_1:70385287-70385306 Msa1211010:three_prime_UTR 50.0%
GAATGAGGATAACCGGAAGG+TGG + chr8_1:70385323-70385342 None:intergenic 50.0%
CCTTCCGGTTATCCTCATTC+CGG - chr8_1:70385323-70385342 Msa1211010:three_prime_UTR 50.0%
! AAAGGGCGTGGATCTGGTAT+AGG - chr8_1:70385913-70385932 Msa1211010:intron 50.0%
AGGAGGATTAATTGCAGGGG+CGG - chr8_1:70385933-70385952 Msa1211010:intron 50.0%
!! TTTGGCAAGCGTTGGAAGCA+TGG - chr8_1:70386048-70386067 Msa1211010:intron 50.0%
! CGTTGGAAGCATGGTAGGTT+TGG - chr8_1:70386057-70386076 Msa1211010:intron 50.0%
CAAGCGATGGAAGTGATGCA+GGG - chr8_1:70386110-70386129 Msa1211010:CDS 50.0%
AAGCGATGGAAGTGATGCAG+GGG - chr8_1:70386111-70386130 Msa1211010:CDS 50.0%
GAATAAGAACCAGGTGGAGG+AGG + chr8_1:70385200-70385219 None:intergenic 50.0%
GTGGATATACCTCCTTGAGG+TGG + chr8_1:70385227-70385246 None:intergenic 50.0%
! AGGAGGTATATCCACCGCAA+CGG - chr8_1:70385232-70385251 Msa1211010:three_prime_UTR 50.0%
TTGAGGTGGATATGCCGTTG+CGG + chr8_1:70385249-70385268 None:intergenic 50.0%
CAATATCCACCACATGCTGC+AGG - chr8_1:70385287-70385306 Msa1211010:three_prime_UTR 50.0%
GAATGAGGATAACCGGAAGG+TGG + chr8_1:70385323-70385342 None:intergenic 50.0%
CCTTCCGGTTATCCTCATTC+CGG - chr8_1:70385323-70385342 Msa1211010:three_prime_UTR 50.0%
! AAAGGGCGTGGATCTGGTAT+AGG - chr8_1:70385913-70385932 Msa1211010:intron 50.0%
AGGAGGATTAATTGCAGGGG+CGG - chr8_1:70385933-70385952 Msa1211010:intron 50.0%
!! TTTGGCAAGCGTTGGAAGCA+TGG - chr8_1:70386048-70386067 Msa1211010:intron 50.0%
! CGTTGGAAGCATGGTAGGTT+TGG - chr8_1:70386057-70386076 Msa1211010:intron 50.0%
CAAGCGATGGAAGTGATGCA+GGG - chr8_1:70386110-70386129 Msa1211010:CDS 50.0%
AAGCGATGGAAGTGATGCAG+GGG - chr8_1:70386111-70386130 Msa1211010:CDS 50.0%
GAACCAGGTGGAGGAGGATA+AGG + chr8_1:70385194-70385213 None:intergenic 55.0%
GCGGTGGATATACCTCCTTG+AGG + chr8_1:70385230-70385249 None:intergenic 55.0%
GCAACGGCATATCCACCTCA+AGG - chr8_1:70385248-70385267 Msa1211010:three_prime_UTR 55.0%
ACGGCATATCCACCTCAAGG+AGG - chr8_1:70385251-70385270 Msa1211010:three_prime_UTR 55.0%
TATCCACCACATGCTGCAGG+AGG - chr8_1:70385290-70385309 Msa1211010:three_prime_UTR 55.0%
CCGGAATGAGGATAACCGGA+AGG + chr8_1:70385326-70385345 None:intergenic 55.0%
CACCATCTGTCGCATGGACA+TGG - chr8_1:70385973-70385992 Msa1211010:intron 55.0%
CTCCATGTCCATGCGACAGA+TGG + chr8_1:70385978-70385997 None:intergenic 55.0%
CATCTGTCGCATGGACATGG+AGG - chr8_1:70385976-70385995 Msa1211010:intron 55.0%
!! GCAAGCGTTGGAAGCATGGT+AGG - chr8_1:70386052-70386071 Msa1211010:intron 55.0%
GCAAGCGATGGAAGTGATGC+AGG - chr8_1:70386109-70386128 Msa1211010:CDS 55.0%
GAACCAGGTGGAGGAGGATA+AGG + chr8_1:70385194-70385213 None:intergenic 55.0%
GCGGTGGATATACCTCCTTG+AGG + chr8_1:70385230-70385249 None:intergenic 55.0%
GCAACGGCATATCCACCTCA+AGG - chr8_1:70385248-70385267 Msa1211010:three_prime_UTR 55.0%
ACGGCATATCCACCTCAAGG+AGG - chr8_1:70385251-70385270 Msa1211010:three_prime_UTR 55.0%
TATCCACCACATGCTGCAGG+AGG - chr8_1:70385290-70385309 Msa1211010:three_prime_UTR 55.0%
CCGGAATGAGGATAACCGGA+AGG + chr8_1:70385326-70385345 None:intergenic 55.0%
CACCATCTGTCGCATGGACA+TGG - chr8_1:70385973-70385992 Msa1211010:intron 55.0%
CTCCATGTCCATGCGACAGA+TGG + chr8_1:70385978-70385997 None:intergenic 55.0%
CATCTGTCGCATGGACATGG+AGG - chr8_1:70385976-70385995 Msa1211010:intron 55.0%
!! GCAAGCGTTGGAAGCATGGT+AGG - chr8_1:70386052-70386071 Msa1211010:intron 55.0%
GCAAGCGATGGAAGTGATGC+AGG - chr8_1:70386109-70386128 Msa1211010:CDS 55.0%
!! GGAGGATAAGGTGGTGCTGC+TGG + chr8_1:70385182-70385201 None:intergenic 60.0%
CCAGGTGGAGGAGGATAAGG+TGG + chr8_1:70385191-70385210 None:intergenic 60.0%
CCACCTTATCCTCCTCCACC+TGG - chr8_1:70385188-70385207 Msa1211010:three_prime_UTR 60.0%
AGGTGGATATGCCGTTGCGG+TGG + chr8_1:70385246-70385265 None:intergenic 60.0%
CACCACATGCTGCAGGAGGA+GGG - chr8_1:70385294-70385313 Msa1211010:three_prime_UTR 60.0%
TACCCTCCTCCTGCAGCATG+TGG + chr8_1:70385299-70385318 None:intergenic 60.0%
!! GGAGGAGGGTATCCACCTTC+CGG - chr8_1:70385308-70385327 Msa1211010:three_prime_UTR 60.0%
GGGCGTGGATCTGGTATAGG+AGG - chr8_1:70385916-70385935 Msa1211010:intron 60.0%
GGTGCTCACCATCTGTCGCA+TGG - chr8_1:70385967-70385986 Msa1211010:intron 60.0%
!! GGAGGATAAGGTGGTGCTGC+TGG + chr8_1:70385182-70385201 None:intergenic 60.0%
CCAGGTGGAGGAGGATAAGG+TGG + chr8_1:70385191-70385210 None:intergenic 60.0%
CCACCTTATCCTCCTCCACC+TGG - chr8_1:70385188-70385207 Msa1211010:three_prime_UTR 60.0%
AGGTGGATATGCCGTTGCGG+TGG + chr8_1:70385246-70385265 None:intergenic 60.0%
CACCACATGCTGCAGGAGGA+GGG - chr8_1:70385294-70385313 Msa1211010:three_prime_UTR 60.0%
TACCCTCCTCCTGCAGCATG+TGG + chr8_1:70385299-70385318 None:intergenic 60.0%
!! GGAGGAGGGTATCCACCTTC+CGG - chr8_1:70385308-70385327 Msa1211010:three_prime_UTR 60.0%
GGGCGTGGATCTGGTATAGG+AGG - chr8_1:70385916-70385935 Msa1211010:intron 60.0%
GGTGCTCACCATCTGTCGCA+TGG - chr8_1:70385967-70385986 Msa1211010:intron 60.0%
CCTCCTCCTGCAGCATGTGG+TGG + chr8_1:70385296-70385315 None:intergenic 65.0%
CCACCACATGCTGCAGGAGG+AGG - chr8_1:70385293-70385312 Msa1211010:three_prime_UTR 65.0%
CCTCCTCCTGCAGCATGTGG+TGG + chr8_1:70385296-70385315 None:intergenic 65.0%
CCACCACATGCTGCAGGAGG+AGG - chr8_1:70385293-70385312 Msa1211010:three_prime_UTR 65.0%
GCAGGGGCGGCAGCTGCATA+CGG - chr8_1:70385946-70385965 Msa1211010:intron 70.0%
GCAGGGGCGGCAGCTGCATA+CGG - chr8_1:70385946-70385965 Msa1211010:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 70385107 70386377 70385107 ID=Msa1211010;Name=Msa1211010
chr8_1 mRNA 70385107 70386377 70385107 ID=Msa1211010-mRNA-1;Parent=Msa1211010;Name=Msa1211010-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|1|0.5|1|1|1|2|252|167
chr8_1 exon 70385107 70385567 70385107 ID=Msa1211010-mRNA-1:exon:23604;Parent=Msa1211010-mRNA-1
chr8_1 exon 70386083 70386377 70386083 ID=Msa1211010-mRNA-1:exon:23603;Parent=Msa1211010-mRNA-1
chr8_1 CDS 70386083 70386377 70386083 ID=Msa1211010-mRNA-1:cds;Parent=Msa1211010-mRNA-1
chr8_1 CDS 70385359 70385567 70385359 ID=Msa1211010-mRNA-1:cds;Parent=Msa1211010-mRNA-1
chr8_1 three_prime_UTR 70385107 70385358 70385107 ID=Msa1211010-mRNA-1:three_prime_utr;Parent=Msa1211010-mRNA-1
Gene Sequence

>Msa1211010

ATGAACAATGGTAGAAACAAGCATGATCAAGAATCTAGTGAGAGAGGTCTGTTTTCACATATAACAGGCTATCCAGCAGCACCACCTTATCCTCCTCCACCTGGTTCTTATTCCTATCCACCTCAAGGAGGTATATCCACCGCAACGGCATATCCACCTCAAGGAGGTTATTATCCTTCACAATATCCACCACATGCTGCAGGAGGAGGGTATCCACCTTCCGGTTATCCTCATTCCGGATATCATCATCAACCATCTTATCATGCTCCACAATATGGCTATCCATATCCTTCTGGGCGTGGATCTGGTATAGGAGGATTAATTGCAGGGGCGGCAGCTGCATACGGTGCTCACCATCTGTCGCATGGACATGGAGGTTATCATCATGGTTATGGTTATGGACATGGGAAGTATAAGCATGGAAAATTTGGCAAGCGTTGGAAGCATGGTAGGTTTGGTTTTGGAAAGTTTAAGCGTGGAAAATTTGGCAAGCGATGGAAGTGA
Protein sequence

>Msa1211010

MNNGRNKHDQESSERGLFSHITGYPAAPPYPPPPGSYSYPPQGGISTATAYPPQGGYYPSQYPPHAAGGGYPPSGYPHSGYHHQPSYHAPQYGYPYPSGRGSGIGGLIAGAAAAYGAHHLSHGHGGYHHGYGYGHGKYKHGKFGKRWKHGRFGFGKFKRGKFGKRWK*