AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0142290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0142290 MtrunA17_Chr4g0075851 53.191 141 28 3 1 103 1 141 5.26e-38 129
Msa0142290 MtrunA17_Chr4g0075841 59.459 74 22 1 110 183 1 66 9.78e-20 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 108 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACTCTTCGTGATAATTT+TGG 0.137277 1_4:+1200512 Msa0142290:three_prime_UTR
TTCACTAGAACAACCGTAAA+AGG 0.199901 1_4:-1200713 None:intergenic
TTTCATCAAATAAATGAAAT+TGG 0.204145 1_4:-1197938 None:intergenic
GACAACTCCACTTCCCTTCT+TGG 0.225568 1_4:-1199690 None:intergenic
CTCACTTGTTCATCTAAATA+TGG 0.252421 1_4:+1199792 Msa0142290:exon
ACCGTAAAAGGGGTCAAATT+TGG 0.254584 1_4:-1200701 None:intergenic
CTACGTTTCCCACTGATAAT+AGG 0.269442 1_4:-1199047 None:intergenic
TAGTGGAGTTGTCACTTCTA+AGG 0.313166 1_4:+1199727 Msa0142290:CDS
GTAATTTAGGACCGTTACTA+TGG 0.345244 1_4:+1198989 Msa0142290:intron
AATAAGCCAAACTTGTTTGT+TGG 0.361245 1_4:+1200155 Msa0142290:three_prime_UTR
CTTTGACCAACAAACAAGTT+TGG 0.361598 1_4:-1200161 None:intergenic
TACAAAACAGAGAAACAAAA+AGG 0.362595 1_4:-1197887 None:intergenic
CCGTAAAAGGGGTCAAATTT+GGG 0.374400 1_4:-1200700 None:intergenic
GTCAGACCCCTTTCCCAACT+TGG 0.382301 1_4:-1200129 None:intergenic
ATCGAAATGCTGATTTGATC+AGG 0.386364 1_4:+1199993 Msa0142290:three_prime_UTR
CAATTCCGATTGAAGCTTGC+CGG 0.387284 1_4:+1198007 Msa0142290:five_prime_UTR
TCAACTTTACTGTTTCAATC+TGG 0.387747 1_4:+1200474 Msa0142290:three_prime_UTR
AAAACTCAAAACTACTGTTA+TGG 0.398181 1_4:+1199076 Msa0142290:CDS
GCCTTAGTCCACCAATCCTT+GGG 0.401731 1_4:-1199878 None:intergenic
CGGCAAGCTTCAATCGGAAT+TGG 0.410141 1_4:-1198006 None:intergenic
AGCCTTAGTCCACCAATCCT+TGG 0.418257 1_4:-1199879 None:intergenic
CTCGTGAATTCAATTGTAGC+TGG 0.420262 1_4:+1199927 Msa0142290:three_prime_UTR
GAAGGAGAAGGAGAAAGAAA+AGG 0.425977 1_4:+1200403 Msa0142290:three_prime_UTR
GCAACAGCTAAAGGCTTTGC+TGG 0.426278 1_4:+1200307 Msa0142290:intron
ATGAAAGAGATAAGGAAGTC+AGG 0.429458 1_4:+1199516 Msa0142290:CDS
ATGCAAAGGGACGCCAAGTT+GGG 0.434365 1_4:+1200116 Msa0142290:three_prime_UTR
TACAGTACAAATCAGTTACT+TGG 0.441479 1_4:-1200646 None:intergenic
CTGCATTGTCTGCAGTGTCC+TGG 0.444540 1_4:-1199154 None:intergenic
TTCTAAAGGTGATCATGTCC+TGG 0.454471 1_4:+1199019 Msa0142290:CDS
GTCAGGATAAATTCAAATCC+TGG 0.455998 1_4:+1199533 Msa0142290:CDS
TGATCCTTTCACTGAAGAGC+TGG 0.462276 1_4:+1199284 Msa0142290:CDS
AAAGAGAACTCAGACTCAGC+TGG 0.468170 1_4:-1197912 None:intergenic
TCACTAGAACAACCGTAAAA+GGG 0.471595 1_4:-1200712 None:intergenic
AATGACACCTGAGATGCTTA+AGG 0.475276 1_4:+1199395 Msa0142290:CDS
TCTACGAATATGAATGTTGA+AGG 0.476392 1_4:-1198103 None:intergenic
GATGCAAAGGGACGCCAAGT+TGG 0.477890 1_4:+1200115 Msa0142290:three_prime_UTR
AGGGACGCCAAGTTGGGAAA+GGG 0.486300 1_4:+1200122 Msa0142290:three_prime_UTR
CTCAAAACTACTGTTATGGA+TGG 0.487863 1_4:+1199080 Msa0142290:CDS
AAGTGGAGTTGTCACTCCTA+AGG 0.488445 1_4:+1199700 Msa0142290:CDS
TCCCAAGGATTGGTGGACTA+AGG 0.492077 1_4:+1199877 Msa0142290:three_prime_UTR
GAATTCAATTGTAGCTGGGC+AGG 0.494104 1_4:+1199932 Msa0142290:three_prime_UTR
AGAAAAGGAAAAGTAGCAGC+AGG 0.496648 1_4:+1200418 Msa0142290:three_prime_UTR
CAGCTAAAGGCTTTGCTGGA+TGG 0.497414 1_4:+1200311 Msa0142290:intron
GCAAGGGAAGTCACATATCC+AGG 0.497708 1_4:+1199136 Msa0142290:CDS
GTCTCCTCCAAGAAGGGAAG+TGG 0.499665 1_4:+1199683 Msa0142290:CDS
ACATGGACAATTGATGCAAA+GGG 0.502711 1_4:+1200103 Msa0142290:three_prime_UTR
AAGGGACGCCAAGTTGGGAA+AGG 0.502741 1_4:+1200121 Msa0142290:three_prime_UTR
AGTGGAGTTGTCACTTCTAA+GGG 0.511110 1_4:+1199728 Msa0142290:CDS
GTCATCAGAATTATTCATAG+TGG 0.511621 1_4:-1200186 None:intergenic
GGAGATGGATGAAAGAGATA+AGG 0.513093 1_4:+1199508 Msa0142290:CDS
AGGGAAGTGGTAGTAGGCAA+GGG 0.515942 1_4:+1199120 Msa0142290:CDS
GAGGGAAGTGGTAGTAGGCA+AGG 0.518111 1_4:+1199119 Msa0142290:CDS
AACTCCACTTCCCTTCTTGG+AGG 0.521516 1_4:-1199687 None:intergenic
TTTAGGACCGTTACTATGGA+GGG 0.521731 1_4:+1198993 Msa0142290:intron
TCAAGGAGGGAAGTGGTAGT+AGG 0.524258 1_4:+1199114 Msa0142290:CDS
AGAGAAGAAGGAGAAGGAGA+AGG 0.525181 1_4:+1200391 Msa0142290:three_prime_UTR
TGGCACCGGCAAGCTTCAAT+CGG 0.525511 1_4:-1198012 None:intergenic
ACAACTCCACTAGCTCCCTT+AGG 0.528809 1_4:-1199716 None:intergenic
CTTTCTCTCATCGCTCTCAA+CGG 0.528933 1_4:+1198138 Msa0142290:five_prime_UTR
AACATGGACAATTGATGCAA+AGG 0.535332 1_4:+1200102 Msa0142290:three_prime_UTR
TCCTGGAGGTCCTAGAAAAG+AGG 0.539090 1_4:+1199550 Msa0142290:CDS
TTTACAAGAGAAGAAGGAGA+AGG 0.541855 1_4:+1200385 Msa0142290:three_prime_UTR
CTGGATGGTAACAAACTGAT+GGG 0.543990 1_4:+1200326 Msa0142290:three_prime_UTR
ACTATGGAGGGAAGTTCTAA+AGG 0.549995 1_4:+1199005 Msa0142290:exon
TGTTACAGATGTTGATGAGC+TGG 0.551297 1_4:-1198032 None:intergenic
TATATATGTAAGTAACTACT+TGG 0.552644 1_4:-1198062 None:intergenic
AGCTCCAGCTCTTCAGTGAA+AGG 0.552717 1_4:-1199288 None:intergenic
GAACTTGTCAAGGAGGGAAG+TGG 0.557621 1_4:+1199107 Msa0142290:CDS
GGGACGCCAAGTTGGGAAAG+GGG 0.563286 1_4:+1200123 Msa0142290:three_prime_UTR
AAGAGTAGTCTCCTCCAAGA+AGG 0.563577 1_4:+1199676 Msa0142290:CDS
TCTAAAGGTGATCATGTCCT+GGG 0.566442 1_4:+1199020 Msa0142290:CDS
AGTGGAGTTGTCACTCCTAA+GGG 0.567341 1_4:+1199701 Msa0142290:CDS
GCTGGATGGTAACAAACTGA+TGG 0.570365 1_4:+1200325 Msa0142290:three_prime_UTR
ACAGGTTAGGTATCTGGAGA+TGG 0.571644 1_4:+1199493 Msa0142290:intron
AGATGTTGATGAGCTGGCAC+CGG 0.571765 1_4:-1198026 None:intergenic
ATTTAGGACCGTTACTATGG+AGG 0.572157 1_4:+1198992 Msa0142290:intron
GATGCACTTGAGATTTCTGA+TGG 0.581693 1_4:+1199572 Msa0142290:CDS
GCTGGAGCTGCAACTTAGAG+AGG 0.583221 1_4:+1199302 Msa0142290:CDS
AAGGGAGCTGGAGGCAACAA+AGG 0.588532 1_4:+1199755 Msa0142290:CDS
AAGAGAACTCAGACTCAGCT+GGG 0.590099 1_4:-1197911 None:intergenic
AGAGTAGTCTCCTCCAAGAA+GGG 0.590436 1_4:+1199677 Msa0142290:CDS
AATATGAATGTTGAAGGAGA+GGG 0.595497 1_4:-1198097 None:intergenic
GGATGGAACTGAACTTGTCA+AGG 0.596328 1_4:+1199097 Msa0142290:CDS
GTCACTCCTAAGGGAGCTAG+TGG 0.597101 1_4:+1199710 Msa0142290:CDS
TGACTCAGCTGTTGACCCCA+TGG 0.598789 1_4:+1200205 Msa0142290:three_prime_UTR
AAATAAACCTGAAGATGAGG+AGG 0.600134 1_4:+1199236 Msa0142290:intron
GAATATGAATGTTGAAGGAG+AGG 0.600142 1_4:-1198098 None:intergenic
CAGGACACTGCAGACAATGC+AGG 0.601280 1_4:+1199155 Msa0142290:CDS
TCGTGAATTCAATTGTAGCT+GGG 0.602234 1_4:+1199928 Msa0142290:three_prime_UTR
TGGGAAAACCTATTATCAGT+GGG 0.604807 1_4:+1199039 Msa0142290:CDS
AGAACTTCCCTCCATAGTAA+CGG 0.605346 1_4:-1199000 None:intergenic
CACGAGCAGCAATTCTCAAG+TGG 0.605488 1_4:-1199910 None:intergenic
CTGGGAAAACCTATTATCAG+TGG 0.606836 1_4:+1199038 Msa0142290:CDS
AAGGAAAAGTAGCAGCAGGA+AGG 0.607126 1_4:+1200422 Msa0142290:three_prime_UTR
CACTAGAACAACCGTAAAAG+GGG 0.608876 1_4:-1200711 None:intergenic
ACAATTGCATTGACTGCACA+AGG 0.614395 1_4:-1200607 None:intergenic
GTGGAGTTGTCACTTCTAAG+GGG 0.614783 1_4:+1199729 Msa0142290:CDS
TCGAAATGCTGATTTGATCA+GGG 0.621010 1_4:+1199994 Msa0142290:three_prime_UTR
TCAAAATGAATAATACTACA+AGG 0.628367 1_4:+1197983 Msa0142290:five_prime_UTR
GGAACTGAACTTGTCAAGGA+GGG 0.640908 1_4:+1199101 Msa0142290:CDS
GTGACTCAAGCAGTAAGACT+CGG 0.647491 1_4:+1199618 Msa0142290:CDS
TGGATGGTAACAAACTGATG+GGG 0.665765 1_4:+1200327 Msa0142290:three_prime_UTR
TGGAACTGAACTTGTCAAGG+AGG 0.668234 1_4:+1199100 Msa0142290:CDS
TGGAGTTGTCACTTCTAAGG+GGG 0.669080 1_4:+1199730 Msa0142290:CDS
GGATGGTAACAAACTGATGG+GGG 0.675199 1_4:+1200328 Msa0142290:three_prime_UTR
TTTGGTAAAACTGATGTGTG+TGG 0.689711 1_4:+1200530 Msa0142290:three_prime_UTR
AGGATAAATTCAAATCCTGG+AGG 0.721340 1_4:+1199536 Msa0142290:CDS
AATGTTACCGAAAAGATGCG+CGG 0.734112 1_4:-1198534 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATTAAAACAAATAAT+TGG - chr1_4:1198701-1198720 None:intergenic 10.0%
!!! TTTTTTGTTAAAATATGATA+TGG + chr1_4:1200263-1200282 Msa0142290:intron 10.0%
!! TTTCATCAAATAAATGAAAT+TGG - chr1_4:1197941-1197960 None:intergenic 15.0%
!!! ATTTTTTAGTCTCTTAAATT+AGG + chr1_4:1198822-1198841 Msa0142290:intron 15.0%
!!! TTTATAATTTTATACAGGTT+AGG + chr1_4:1199480-1199499 Msa0142290:intron 15.0%
!! AAGCAAAATAAATTAAATGA+AGG - chr1_4:1200052-1200071 None:intergenic 15.0%
!!! TTTTTTCTATAGATTAAACA+TGG + chr1_4:1200086-1200105 Msa0142290:intron 15.0%
!! AAATTATGAAAACTTTCATA+GGG - chr1_4:1200241-1200260 None:intergenic 15.0%
!! AAAATTATGAAAACTTTCAT+AGG - chr1_4:1200242-1200261 None:intergenic 15.0%
!! TCAAAATGAATAATACTACA+AGG + chr1_4:1197983-1198002 Msa0142290:five_prime_UTR 20.0%
!! TATATATGTAAGTAACTACT+TGG - chr1_4:1198065-1198084 None:intergenic 20.0%
!! AGGATTCATGAAAATAAAAA+CGG - chr1_4:1198358-1198377 None:intergenic 20.0%
!! TAGATTATACTCAAAGATTA+TGG - chr1_4:1198959-1198978 None:intergenic 20.0%
!! CTATGTATTTGATGTAATTT+AGG + chr1_4:1198976-1198995 Msa0142290:intron 20.0%
!! CAACATTATCTATTCAATTA+AGG - chr1_4:1199438-1199457 None:intergenic 20.0%
!! TTGAATCTTCTTAATACAAT+AGG + chr1_4:1200023-1200042 Msa0142290:intron 20.0%
! TACAAAACAGAGAAACAAAA+AGG - chr1_4:1197890-1197909 None:intergenic 25.0%
! GATGATTAATGTTATCGTAA+AGG - chr1_4:1198277-1198296 None:intergenic 25.0%
! TCGTTGTTAATCAATTAAAG+TGG - chr1_4:1198787-1198806 None:intergenic 25.0%
! TATCAACAATCTTGAACAAA+AGG - chr1_4:1198878-1198897 None:intergenic 25.0%
! AAAACTCAAAACTACTGTTA+TGG + chr1_4:1199076-1199095 Msa0142290:CDS 25.0%
!!! TTATTTCTGAAGTTAATAGC+AGG - chr1_4:1199222-1199241 None:intergenic 25.0%
!!! CTATTGAATTGAATTTTGAC+TGG + chr1_4:1199328-1199347 Msa0142290:CDS 25.0%
!!! ATAGGGTATTTTTTTACCAT+GGG - chr1_4:1200224-1200243 None:intergenic 25.0%
!! TGAAATTTTACAAGAGAAGA+AGG + chr1_4:1200379-1200398 Msa0142290:three_prime_UTR 25.0%
AGAAACAAAAAGGCAAAGAA+AGG - chr1_4:1197880-1197899 None:intergenic 30.0%
AATATGAATGTTGAAGGAGA+GGG - chr1_4:1198100-1198119 None:intergenic 30.0%
TCTACGAATATGAATGTTGA+AGG - chr1_4:1198106-1198125 None:intergenic 30.0%
AAGGATTCATGAAAACGAAA+AGG - chr1_4:1198218-1198237 None:intergenic 30.0%
TTAGGCATTCTTCATCATTA+CGG + chr1_4:1198412-1198431 Msa0142290:intron 30.0%
!!! CAGTAGTTTTGAGTTTTCTT+TGG - chr1_4:1199073-1199092 None:intergenic 30.0%
!!! TTGAATTGAATTTTGACTGG+AGG + chr1_4:1199331-1199350 Msa0142290:CDS 30.0%
! TATTTTACCTTAAGCATCTC+AGG - chr1_4:1199405-1199424 None:intergenic 30.0%
! TTTTATACAGGTTAGGTATC+TGG + chr1_4:1199487-1199506 Msa0142290:intron 30.0%
CTCACTTGTTCATCTAAATA+TGG + chr1_4:1199792-1199811 Msa0142290:exon 30.0%
TAGTTAAAAATCCCAAGGAT+TGG + chr1_4:1199867-1199886 Msa0142290:three_prime_UTR 30.0%
! AATAAGCCAAACTTGTTTGT+TGG + chr1_4:1200155-1200174 Msa0142290:three_prime_UTR 30.0%
GTCATCAGAATTATTCATAG+TGG - chr1_4:1200189-1200208 None:intergenic 30.0%
!!! TAGGGTATTTTTTTACCATG+GGG - chr1_4:1200223-1200242 None:intergenic 30.0%
!!! CATAGGGTATTTTTTTACCA+TGG - chr1_4:1200225-1200244 None:intergenic 30.0%
!!! TTTTTTGTTGCAACAGCTAA+AGG + chr1_4:1200298-1200317 Msa0142290:intron 30.0%
TCAACTTTACTGTTTCAATC+TGG + chr1_4:1200474-1200493 Msa0142290:three_prime_UTR 30.0%
! CAAACTCTTCGTGATAATTT+TGG + chr1_4:1200512-1200531 Msa0142290:three_prime_UTR 30.0%
TACAGTACAAATCAGTTACT+TGG - chr1_4:1200649-1200668 None:intergenic 30.0%
GAATATGAATGTTGAAGGAG+AGG - chr1_4:1198101-1198120 None:intergenic 35.0%
! TCTCAACGTTTTCATCTTTC+AGG + chr1_4:1198170-1198189 Msa0142290:five_prime_UTR 35.0%
CATCAACAACAACAACAACA+AGG - chr1_4:1198237-1198256 None:intergenic 35.0%
GATGCTGATGAATCTTTCTT+CGG + chr1_4:1198321-1198340 Msa0142290:intron 35.0%
TTCACTGATGAATCTTCGTT+CGG + chr1_4:1198380-1198399 Msa0142290:intron 35.0%
! ATGAATCTTCGTTCGGTTTT+AGG + chr1_4:1198387-1198406 Msa0142290:intron 35.0%
!!! TTCGTTCGGTTTTAGGTTTT+AGG + chr1_4:1198394-1198413 Msa0142290:intron 35.0%
ATGAATCTTCGTTCGATTTC+AGG + chr1_4:1198491-1198510 Msa0142290:intron 35.0%
! GTAATTTAGGACCGTTACTA+TGG + chr1_4:1198989-1199008 Msa0142290:intron 35.0%
TGGGAAAACCTATTATCAGT+GGG + chr1_4:1199039-1199058 Msa0142290:CDS 35.0%
CTCAAAACTACTGTTATGGA+TGG + chr1_4:1199080-1199099 Msa0142290:CDS 35.0%
CAGAAATAAACCTGAAGATG+AGG + chr1_4:1199233-1199252 Msa0142290:intron 35.0%
AAATAAACCTGAAGATGAGG+AGG + chr1_4:1199236-1199255 Msa0142290:intron 35.0%
ATGAAAGAGATAAGGAAGTC+AGG + chr1_4:1199516-1199535 Msa0142290:CDS 35.0%
GTCAGGATAAATTCAAATCC+TGG + chr1_4:1199533-1199552 Msa0142290:CDS 35.0%
AGGATAAATTCAAATCCTGG+AGG + chr1_4:1199536-1199555 Msa0142290:CDS 35.0%
AAGCGTAGTTAAAAATCCCA+AGG + chr1_4:1199862-1199881 Msa0142290:three_prime_UTR 35.0%
TTAAAAATCCCAAGGATTGG+TGG + chr1_4:1199870-1199889 Msa0142290:three_prime_UTR 35.0%
TCGTGAATTCAATTGTAGCT+GGG + chr1_4:1199928-1199947 Msa0142290:three_prime_UTR 35.0%
!! ATCGAAATGCTGATTTGATC+AGG + chr1_4:1199993-1200012 Msa0142290:three_prime_UTR 35.0%
!! TCGAAATGCTGATTTGATCA+GGG + chr1_4:1199994-1200013 Msa0142290:three_prime_UTR 35.0%
AACATGGACAATTGATGCAA+AGG + chr1_4:1200102-1200121 Msa0142290:three_prime_UTR 35.0%
ACATGGACAATTGATGCAAA+GGG + chr1_4:1200103-1200122 Msa0142290:three_prime_UTR 35.0%
CTTTGACCAACAAACAAGTT+TGG - chr1_4:1200164-1200183 None:intergenic 35.0%
TTTACAAGAGAAGAAGGAGA+AGG + chr1_4:1200385-1200404 Msa0142290:three_prime_UTR 35.0%
TTTGGTAAAACTGATGTGTG+TGG + chr1_4:1200530-1200549 Msa0142290:three_prime_UTR 35.0%
TCACTAGAACAACCGTAAAA+GGG - chr1_4:1200715-1200734 None:intergenic 35.0%
TTCACTAGAACAACCGTAAA+AGG - chr1_4:1200716-1200735 None:intergenic 35.0%
! TGTTACAGATGTTGATGAGC+TGG - chr1_4:1198035-1198054 None:intergenic 40.0%
CGAAGATTCATCAGTGAACA+AGG - chr1_4:1198378-1198397 None:intergenic 40.0%
CGAAGATTCATCAGTGAACA+AGG - chr1_4:1198378-1198397 None:intergenic 40.0%
AATGTTACCGAAAAGATGCG+CGG - chr1_4:1198537-1198556 None:intergenic 40.0%
! ATTTAGGACCGTTACTATGG+AGG + chr1_4:1198992-1199011 Msa0142290:intron 40.0%
! TTTAGGACCGTTACTATGGA+GGG + chr1_4:1198993-1199012 Msa0142290:intron 40.0%
AGAACTTCCCTCCATAGTAA+CGG - chr1_4:1199003-1199022 None:intergenic 40.0%
! ACTATGGAGGGAAGTTCTAA+AGG + chr1_4:1199005-1199024 Msa0142290:exon 40.0%
! TTCTAAAGGTGATCATGTCC+TGG + chr1_4:1199019-1199038 Msa0142290:CDS 40.0%
TCTAAAGGTGATCATGTCCT+GGG + chr1_4:1199020-1199039 Msa0142290:CDS 40.0%
! CACTGATAATAGGTTTTCCC+AGG - chr1_4:1199040-1199059 None:intergenic 40.0%
CTGGGAAAACCTATTATCAG+TGG + chr1_4:1199038-1199057 Msa0142290:CDS 40.0%
CTACGTTTCCCACTGATAAT+AGG - chr1_4:1199050-1199069 None:intergenic 40.0%
AATGACACCTGAGATGCTTA+AGG + chr1_4:1199395-1199414 Msa0142290:CDS 40.0%
GGAGATGGATGAAAGAGATA+AGG + chr1_4:1199508-1199527 Msa0142290:CDS 40.0%
! TCAAGTGCATCCTCTTTTCT+AGG - chr1_4:1199563-1199582 None:intergenic 40.0%
GATGCACTTGAGATTTCTGA+TGG + chr1_4:1199572-1199591 Msa0142290:CDS 40.0%
! TAGTGGAGTTGTCACTTCTA+AGG + chr1_4:1199727-1199746 Msa0142290:CDS 40.0%
! AGTGGAGTTGTCACTTCTAA+GGG + chr1_4:1199728-1199747 Msa0142290:CDS 40.0%
CTCGTGAATTCAATTGTAGC+TGG + chr1_4:1199927-1199946 Msa0142290:three_prime_UTR 40.0%
CTGGATGGTAACAAACTGAT+GGG + chr1_4:1200326-1200345 Msa0142290:three_prime_UTR 40.0%
TGGATGGTAACAAACTGATG+GGG + chr1_4:1200327-1200346 Msa0142290:three_prime_UTR 40.0%
GAAGGAGAAGGAGAAAGAAA+AGG + chr1_4:1200403-1200422 Msa0142290:three_prime_UTR 40.0%
AGAAAAGGAAAAGTAGCAGC+AGG + chr1_4:1200418-1200437 Msa0142290:three_prime_UTR 40.0%
!! ACAATTGCATTGACTGCACA+AGG - chr1_4:1200610-1200629 None:intergenic 40.0%
CCGTAAAAGGGGTCAAATTT+GGG - chr1_4:1200703-1200722 None:intergenic 40.0%
! CCCAAATTTGACCCCTTTTA+CGG + chr1_4:1200700-1200719 Msa0142290:three_prime_UTR 40.0%
ACCGTAAAAGGGGTCAAATT+TGG - chr1_4:1200704-1200723 None:intergenic 40.0%
CACTAGAACAACCGTAAAAG+GGG - chr1_4:1200714-1200733 None:intergenic 40.0%
AAGAGAACTCAGACTCAGCT+GGG - chr1_4:1197914-1197933 None:intergenic 45.0%
AAAGAGAACTCAGACTCAGC+TGG - chr1_4:1197915-1197934 None:intergenic 45.0%
CAATTCCGATTGAAGCTTGC+CGG + chr1_4:1198007-1198026 Msa0142290:five_prime_UTR 45.0%
CTTTCTCTCATCGCTCTCAA+CGG + chr1_4:1198138-1198157 Msa0142290:five_prime_UTR 45.0%
! CATCTTTCCGCGCATCTTTT+CGG + chr1_4:1198527-1198546 Msa0142290:five_prime_UTR 45.0%
GGATGGAACTGAACTTGTCA+AGG + chr1_4:1199097-1199116 Msa0142290:CDS 45.0%
TGGAACTGAACTTGTCAAGG+AGG + chr1_4:1199100-1199119 Msa0142290:CDS 45.0%
GGAACTGAACTTGTCAAGGA+GGG + chr1_4:1199101-1199120 Msa0142290:CDS 45.0%
TGATCCTTTCACTGAAGAGC+TGG + chr1_4:1199284-1199303 Msa0142290:CDS 45.0%
ACAGGTTAGGTATCTGGAGA+TGG + chr1_4:1199493-1199512 Msa0142290:intron 45.0%
GTGACTCAAGCAGTAAGACT+CGG + chr1_4:1199618-1199637 Msa0142290:CDS 45.0%
AAGAGTAGTCTCCTCCAAGA+AGG + chr1_4:1199676-1199695 Msa0142290:CDS 45.0%
AGAGTAGTCTCCTCCAAGAA+GGG + chr1_4:1199677-1199696 Msa0142290:CDS 45.0%
AAGTGGAGTTGTCACTCCTA+AGG + chr1_4:1199700-1199719 Msa0142290:CDS 45.0%
AGTGGAGTTGTCACTCCTAA+GGG + chr1_4:1199701-1199720 Msa0142290:CDS 45.0%
! GTGGAGTTGTCACTTCTAAG+GGG + chr1_4:1199729-1199748 Msa0142290:CDS 45.0%
! TGGAGTTGTCACTTCTAAGG+GGG + chr1_4:1199730-1199749 Msa0142290:CDS 45.0%
GAATTCAATTGTAGCTGGGC+AGG + chr1_4:1199932-1199951 Msa0142290:three_prime_UTR 45.0%
GCTGGATGGTAACAAACTGA+TGG + chr1_4:1200325-1200344 Msa0142290:three_prime_UTR 45.0%
GGATGGTAACAAACTGATGG+GGG + chr1_4:1200328-1200347 Msa0142290:three_prime_UTR 45.0%
AGAGAAGAAGGAGAAGGAGA+AGG + chr1_4:1200391-1200410 Msa0142290:three_prime_UTR 45.0%
AAGGAAAAGTAGCAGCAGGA+AGG + chr1_4:1200422-1200441 Msa0142290:three_prime_UTR 45.0%
!!! AATTATTTATAATTTTATAC+AGG + chr1_4:1199475-1199494 Msa0142290:intron 5.0%
CGGCAAGCTTCAATCGGAAT+TGG - chr1_4:1198009-1198028 None:intergenic 50.0%
! AGATGTTGATGAGCTGGCAC+CGG - chr1_4:1198029-1198048 None:intergenic 50.0%
GAACTTGTCAAGGAGGGAAG+TGG + chr1_4:1199107-1199126 Msa0142290:CDS 50.0%
TCAAGGAGGGAAGTGGTAGT+AGG + chr1_4:1199114-1199133 Msa0142290:CDS 50.0%
AGGGAAGTGGTAGTAGGCAA+GGG + chr1_4:1199120-1199139 Msa0142290:CDS 50.0%
GCAAGGGAAGTCACATATCC+AGG + chr1_4:1199136-1199155 Msa0142290:CDS 50.0%
AGCTCCAGCTCTTCAGTGAA+AGG - chr1_4:1199291-1199310 None:intergenic 50.0%
TCCTGGAGGTCCTAGAAAAG+AGG + chr1_4:1199550-1199569 Msa0142290:CDS 50.0%
!!! TCCTCTTTTCTAGGACCTCC+AGG - chr1_4:1199554-1199573 None:intergenic 50.0%
AACTCCACTTCCCTTCTTGG+AGG - chr1_4:1199690-1199709 None:intergenic 50.0%
GACAACTCCACTTCCCTTCT+TGG - chr1_4:1199693-1199712 None:intergenic 50.0%
ACAACTCCACTAGCTCCCTT+AGG - chr1_4:1199719-1199738 None:intergenic 50.0%
! TGTCACTTCTAAGGGGGCTA+AGG + chr1_4:1199736-1199755 Msa0142290:CDS 50.0%
! GTCACTTCTAAGGGGGCTAA+GGG + chr1_4:1199737-1199756 Msa0142290:CDS 50.0%
!! TCCCAAGGATTGGTGGACTA+AGG + chr1_4:1199877-1199896 Msa0142290:three_prime_UTR 50.0%
GCCTTAGTCCACCAATCCTT+GGG - chr1_4:1199881-1199900 None:intergenic 50.0%
AGCCTTAGTCCACCAATCCT+TGG - chr1_4:1199882-1199901 None:intergenic 50.0%
CACGAGCAGCAATTCTCAAG+TGG - chr1_4:1199913-1199932 None:intergenic 50.0%
ATGCAAAGGGACGCCAAGTT+GGG + chr1_4:1200116-1200135 Msa0142290:three_prime_UTR 50.0%
GCAACAGCTAAAGGCTTTGC+TGG + chr1_4:1200307-1200326 Msa0142290:intron 50.0%
CAGCTAAAGGCTTTGCTGGA+TGG + chr1_4:1200311-1200330 Msa0142290:intron 50.0%
!! TGGCACCGGCAAGCTTCAAT+CGG - chr1_4:1198015-1198034 None:intergenic 55.0%
GAGGGAAGTGGTAGTAGGCA+AGG + chr1_4:1199119-1199138 Msa0142290:CDS 55.0%
CTGCATTGTCTGCAGTGTCC+TGG - chr1_4:1199157-1199176 None:intergenic 55.0%
CAGGACACTGCAGACAATGC+AGG + chr1_4:1199155-1199174 Msa0142290:CDS 55.0%
CACAGCTCCTCCTCATCTTC+AGG - chr1_4:1199246-1199265 None:intergenic 55.0%
GCTGGAGCTGCAACTTAGAG+AGG + chr1_4:1199302-1199321 Msa0142290:CDS 55.0%
GTCTCCTCCAAGAAGGGAAG+TGG + chr1_4:1199683-1199702 Msa0142290:CDS 55.0%
GTCACTCCTAAGGGAGCTAG+TGG + chr1_4:1199710-1199729 Msa0142290:CDS 55.0%
AAGGGAGCTGGAGGCAACAA+AGG + chr1_4:1199755-1199774 Msa0142290:CDS 55.0%
GATGCAAAGGGACGCCAAGT+TGG + chr1_4:1200115-1200134 Msa0142290:three_prime_UTR 55.0%
! AAGGGACGCCAAGTTGGGAA+AGG + chr1_4:1200121-1200140 Msa0142290:three_prime_UTR 55.0%
! AGGGACGCCAAGTTGGGAAA+GGG + chr1_4:1200122-1200141 Msa0142290:three_prime_UTR 55.0%
GTCAGACCCCTTTCCCAACT+TGG - chr1_4:1200132-1200151 None:intergenic 55.0%
TGACTCAGCTGTTGACCCCA+TGG + chr1_4:1200205-1200224 Msa0142290:three_prime_UTR 55.0%
TCTAAGGGGGCTAAGGGAGC+TGG + chr1_4:1199743-1199762 Msa0142290:CDS 60.0%
! GGGACGCCAAGTTGGGAAAG+GGG + chr1_4:1200123-1200142 Msa0142290:three_prime_UTR 60.0%
AAGGGGGCTAAGGGAGCTGG+AGG + chr1_4:1199746-1199765 Msa0142290:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 1197852 1200755 1197852 ID=Msa0142290;Name=Msa0142290
chr1_4 mRNA 1197852 1200755 1197852 ID=Msa0142290-mRNA-1;Parent=Msa0142290;Name=Msa0142290-mRNA-1;_AED=0.09;_eAED=0.09;_QI=386|1|1|1|0.66|0.71|7|778|215
chr1_4 exon 1197852 1198191 1197852 ID=Msa0142290-mRNA-1:exon:377;Parent=Msa0142290-mRNA-1
chr1_4 exon 1198513 1198548 1198513 ID=Msa0142290-mRNA-1:exon:378;Parent=Msa0142290-mRNA-1
chr1_4 exon 1198998 1199176 1198998 ID=Msa0142290-mRNA-1:exon:379;Parent=Msa0142290-mRNA-1
chr1_4 exon 1199250 1199416 1199250 ID=Msa0142290-mRNA-1:exon:380;Parent=Msa0142290-mRNA-1
chr1_4 exon 1199497 1200015 1199497 ID=Msa0142290-mRNA-1:exon:381;Parent=Msa0142290-mRNA-1
chr1_4 exon 1200098 1200226 1200098 ID=Msa0142290-mRNA-1:exon:382;Parent=Msa0142290-mRNA-1
chr1_4 exon 1200314 1200755 1200314 ID=Msa0142290-mRNA-1:exon:383;Parent=Msa0142290-mRNA-1
chr1_4 five_prime_UTR 1197852 1198191 1197852 ID=Msa0142290-mRNA-1:five_prime_utr;Parent=Msa0142290-mRNA-1
chr1_4 five_prime_UTR 1198513 1198548 1198513 ID=Msa0142290-mRNA-1:five_prime_utr;Parent=Msa0142290-mRNA-1
chr1_4 five_prime_UTR 1198998 1199007 1198998 ID=Msa0142290-mRNA-1:five_prime_utr;Parent=Msa0142290-mRNA-1
chr1_4 CDS 1199008 1199176 1199008 ID=Msa0142290-mRNA-1:cds;Parent=Msa0142290-mRNA-1
chr1_4 CDS 1199250 1199416 1199250 ID=Msa0142290-mRNA-1:cds;Parent=Msa0142290-mRNA-1
chr1_4 CDS 1199497 1199808 1199497 ID=Msa0142290-mRNA-1:cds;Parent=Msa0142290-mRNA-1
chr1_4 three_prime_UTR 1199809 1200015 1199809 ID=Msa0142290-mRNA-1:three_prime_utr;Parent=Msa0142290-mRNA-1
chr1_4 three_prime_UTR 1200098 1200226 1200098 ID=Msa0142290-mRNA-1:three_prime_utr;Parent=Msa0142290-mRNA-1
chr1_4 three_prime_UTR 1200314 1200755 1200314 ID=Msa0142290-mRNA-1:three_prime_utr;Parent=Msa0142290-mRNA-1
Gene Sequence

>Msa0142290

ATGGAGGGAAGTTCTAAAGGTGATCATGTCCTGGGAAAACCTATTATCAGTGGGAAACGTAGCCAAAGAAAACTCAAAACTACTGTTATGGATGGAACTGAACTTGTCAAGGAGGGAAGTGGTAGTAGGCAAGGGAAGTCACATATCCAGGACACTGCAGACAATGCAGATGAGGAGGAGCTGTGCGATTCATTGCTTTTTGTTGATCCTTTCACTGAAGAGCTGGAGCTGCAACTTAGAGAGGCTGCTATTGAATTGAATTTTGACTGGAGGAAGATCAAGAGATCTAAAAAATATGCTAATATTTTTAAAGAAATGACACCTGAGATGCTTAAGGTTAGGTATCTGGAGATGGATGAAAGAGATAAGGAAGTCAGGATAAATTCAAATCCTGGAGGTCCTAGAAAAGAGGATGCACTTGAGATTTCTGATGGTAGCTCAAGCAGAAAGACTGATAGTGACTCAAGCAGTAAGACTCGGACAGATGATGATTCTATTCAGCTGAGAAGAAGTGCAAGAGTAGTCTCCTCCAAGAAGGGAAGTGGAGTTGTCACTCCTAAGGGAGCTAGTGGAGTTGTCACTTCTAAGGGGGCTAAGGGAGCTGGAGGCAACAAAGGTTCTCTTACATTCACTCACTTGTTCATCTAA
Protein sequence

>Msa0142290

MEGSSKGDHVLGKPIISGKRSQRKLKTTVMDGTELVKEGSGSRQGKSHIQDTADNADEEELCDSLLFVDPFTEELELQLREAAIELNFDWRKIKRSKKYANIFKEMTPEMLKVRYLEMDERDKEVRINSNPGGPRKEDALEISDGSSSRKTDSDSSSKTRTDDDSIQLRRSARVVSSKKGSGVVTPKGASGVVTSKGAKGAGGNKGSLTFTHLFI*