AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1224210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1224210 MtrunA17_Chr4g0075851 53.191 141 28 3 1 103 1 141 5.26e-38 129
Msa1224210 MtrunA17_Chr4g0075841 59.459 74 22 1 110 183 1 66 9.78e-20 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 108 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACTCTTCGTGATAATTT+TGG 0.137277 8_1:+89002002 Msa1224210:three_prime_UTR
TTCACTAGAACAACCGTAAA+AGG 0.199901 8_1:-89002203 None:intergenic
TTTCATCAAATAAATGAAAT+TGG 0.204145 8_1:-88999428 None:intergenic
GACAACTCCACTTCCCTTCT+TGG 0.225568 8_1:-89001180 None:intergenic
CTCACTTGTTCATCTAAATA+TGG 0.252421 8_1:+89001282 Msa1224210:exon
ACCGTAAAAGGGGTCAAATT+TGG 0.254584 8_1:-89002191 None:intergenic
CTACGTTTCCCACTGATAAT+AGG 0.269442 8_1:-89000537 None:intergenic
TAGTGGAGTTGTCACTTCTA+AGG 0.313166 8_1:+89001217 Msa1224210:CDS
GTAATTTAGGACCGTTACTA+TGG 0.345244 8_1:+89000479 Msa1224210:intron
AATAAGCCAAACTTGTTTGT+TGG 0.361245 8_1:+89001645 Msa1224210:three_prime_UTR
CTTTGACCAACAAACAAGTT+TGG 0.361598 8_1:-89001651 None:intergenic
TACAAAACAGAGAAACAAAA+AGG 0.362595 8_1:-88999377 None:intergenic
CCGTAAAAGGGGTCAAATTT+GGG 0.374400 8_1:-89002190 None:intergenic
GTCAGACCCCTTTCCCAACT+TGG 0.382301 8_1:-89001619 None:intergenic
ATCGAAATGCTGATTTGATC+AGG 0.386364 8_1:+89001483 Msa1224210:three_prime_UTR
CAATTCCGATTGAAGCTTGC+CGG 0.387284 8_1:+88999497 Msa1224210:five_prime_UTR
TCAACTTTACTGTTTCAATC+TGG 0.387747 8_1:+89001964 Msa1224210:three_prime_UTR
AAAACTCAAAACTACTGTTA+TGG 0.398181 8_1:+89000566 Msa1224210:CDS
GCCTTAGTCCACCAATCCTT+GGG 0.401731 8_1:-89001368 None:intergenic
CGGCAAGCTTCAATCGGAAT+TGG 0.410141 8_1:-88999496 None:intergenic
AGCCTTAGTCCACCAATCCT+TGG 0.418257 8_1:-89001369 None:intergenic
CTCGTGAATTCAATTGTAGC+TGG 0.420262 8_1:+89001417 Msa1224210:three_prime_UTR
GAAGGAGAAGGAGAAAGAAA+AGG 0.425977 8_1:+89001893 Msa1224210:three_prime_UTR
GCAACAGCTAAAGGCTTTGC+TGG 0.426278 8_1:+89001797 Msa1224210:intron
ATGAAAGAGATAAGGAAGTC+AGG 0.429458 8_1:+89001006 Msa1224210:CDS
ATGCAAAGGGACGCCAAGTT+GGG 0.434365 8_1:+89001606 Msa1224210:three_prime_UTR
TACAGTACAAATCAGTTACT+TGG 0.441479 8_1:-89002136 None:intergenic
CTGCATTGTCTGCAGTGTCC+TGG 0.444540 8_1:-89000644 None:intergenic
TTCTAAAGGTGATCATGTCC+TGG 0.454471 8_1:+89000509 Msa1224210:CDS
GTCAGGATAAATTCAAATCC+TGG 0.455998 8_1:+89001023 Msa1224210:CDS
TGATCCTTTCACTGAAGAGC+TGG 0.462276 8_1:+89000774 Msa1224210:CDS
AAAGAGAACTCAGACTCAGC+TGG 0.468170 8_1:-88999402 None:intergenic
TCACTAGAACAACCGTAAAA+GGG 0.471595 8_1:-89002202 None:intergenic
AATGACACCTGAGATGCTTA+AGG 0.475276 8_1:+89000885 Msa1224210:CDS
TCTACGAATATGAATGTTGA+AGG 0.476392 8_1:-88999593 None:intergenic
GATGCAAAGGGACGCCAAGT+TGG 0.477890 8_1:+89001605 Msa1224210:three_prime_UTR
AGGGACGCCAAGTTGGGAAA+GGG 0.486300 8_1:+89001612 Msa1224210:three_prime_UTR
CTCAAAACTACTGTTATGGA+TGG 0.487863 8_1:+89000570 Msa1224210:CDS
AAGTGGAGTTGTCACTCCTA+AGG 0.488445 8_1:+89001190 Msa1224210:CDS
TCCCAAGGATTGGTGGACTA+AGG 0.492077 8_1:+89001367 Msa1224210:three_prime_UTR
GAATTCAATTGTAGCTGGGC+AGG 0.494104 8_1:+89001422 Msa1224210:three_prime_UTR
AGAAAAGGAAAAGTAGCAGC+AGG 0.496648 8_1:+89001908 Msa1224210:three_prime_UTR
CAGCTAAAGGCTTTGCTGGA+TGG 0.497414 8_1:+89001801 Msa1224210:intron
GCAAGGGAAGTCACATATCC+AGG 0.497708 8_1:+89000626 Msa1224210:CDS
GTCTCCTCCAAGAAGGGAAG+TGG 0.499665 8_1:+89001173 Msa1224210:CDS
ACATGGACAATTGATGCAAA+GGG 0.502711 8_1:+89001593 Msa1224210:three_prime_UTR
AAGGGACGCCAAGTTGGGAA+AGG 0.502741 8_1:+89001611 Msa1224210:three_prime_UTR
AGTGGAGTTGTCACTTCTAA+GGG 0.511110 8_1:+89001218 Msa1224210:CDS
GTCATCAGAATTATTCATAG+TGG 0.511621 8_1:-89001676 None:intergenic
GGAGATGGATGAAAGAGATA+AGG 0.513093 8_1:+89000998 Msa1224210:CDS
AGGGAAGTGGTAGTAGGCAA+GGG 0.515942 8_1:+89000610 Msa1224210:CDS
GAGGGAAGTGGTAGTAGGCA+AGG 0.518111 8_1:+89000609 Msa1224210:CDS
AACTCCACTTCCCTTCTTGG+AGG 0.521516 8_1:-89001177 None:intergenic
TTTAGGACCGTTACTATGGA+GGG 0.521731 8_1:+89000483 Msa1224210:intron
TCAAGGAGGGAAGTGGTAGT+AGG 0.524258 8_1:+89000604 Msa1224210:CDS
AGAGAAGAAGGAGAAGGAGA+AGG 0.525181 8_1:+89001881 Msa1224210:three_prime_UTR
TGGCACCGGCAAGCTTCAAT+CGG 0.525511 8_1:-88999502 None:intergenic
ACAACTCCACTAGCTCCCTT+AGG 0.528809 8_1:-89001206 None:intergenic
CTTTCTCTCATCGCTCTCAA+CGG 0.528933 8_1:+88999628 Msa1224210:five_prime_UTR
AACATGGACAATTGATGCAA+AGG 0.535332 8_1:+89001592 Msa1224210:three_prime_UTR
TCCTGGAGGTCCTAGAAAAG+AGG 0.539090 8_1:+89001040 Msa1224210:CDS
TTTACAAGAGAAGAAGGAGA+AGG 0.541855 8_1:+89001875 Msa1224210:three_prime_UTR
CTGGATGGTAACAAACTGAT+GGG 0.543990 8_1:+89001816 Msa1224210:three_prime_UTR
ACTATGGAGGGAAGTTCTAA+AGG 0.549995 8_1:+89000495 Msa1224210:exon
TGTTACAGATGTTGATGAGC+TGG 0.551297 8_1:-88999522 None:intergenic
TATATATGTAAGTAACTACT+TGG 0.552644 8_1:-88999552 None:intergenic
AGCTCCAGCTCTTCAGTGAA+AGG 0.552717 8_1:-89000778 None:intergenic
GAACTTGTCAAGGAGGGAAG+TGG 0.557621 8_1:+89000597 Msa1224210:CDS
GGGACGCCAAGTTGGGAAAG+GGG 0.563286 8_1:+89001613 Msa1224210:three_prime_UTR
AAGAGTAGTCTCCTCCAAGA+AGG 0.563577 8_1:+89001166 Msa1224210:CDS
TCTAAAGGTGATCATGTCCT+GGG 0.566442 8_1:+89000510 Msa1224210:CDS
AGTGGAGTTGTCACTCCTAA+GGG 0.567341 8_1:+89001191 Msa1224210:CDS
GCTGGATGGTAACAAACTGA+TGG 0.570365 8_1:+89001815 Msa1224210:three_prime_UTR
ACAGGTTAGGTATCTGGAGA+TGG 0.571644 8_1:+89000983 Msa1224210:intron
AGATGTTGATGAGCTGGCAC+CGG 0.571765 8_1:-88999516 None:intergenic
ATTTAGGACCGTTACTATGG+AGG 0.572157 8_1:+89000482 Msa1224210:intron
GATGCACTTGAGATTTCTGA+TGG 0.581693 8_1:+89001062 Msa1224210:CDS
GCTGGAGCTGCAACTTAGAG+AGG 0.583221 8_1:+89000792 Msa1224210:CDS
AAGGGAGCTGGAGGCAACAA+AGG 0.588532 8_1:+89001245 Msa1224210:CDS
AAGAGAACTCAGACTCAGCT+GGG 0.590099 8_1:-88999401 None:intergenic
AGAGTAGTCTCCTCCAAGAA+GGG 0.590436 8_1:+89001167 Msa1224210:CDS
AATATGAATGTTGAAGGAGA+GGG 0.595497 8_1:-88999587 None:intergenic
GGATGGAACTGAACTTGTCA+AGG 0.596328 8_1:+89000587 Msa1224210:CDS
GTCACTCCTAAGGGAGCTAG+TGG 0.597101 8_1:+89001200 Msa1224210:CDS
TGACTCAGCTGTTGACCCCA+TGG 0.598789 8_1:+89001695 Msa1224210:three_prime_UTR
AAATAAACCTGAAGATGAGG+AGG 0.600134 8_1:+89000726 Msa1224210:intron
GAATATGAATGTTGAAGGAG+AGG 0.600142 8_1:-88999588 None:intergenic
CAGGACACTGCAGACAATGC+AGG 0.601280 8_1:+89000645 Msa1224210:CDS
TCGTGAATTCAATTGTAGCT+GGG 0.602234 8_1:+89001418 Msa1224210:three_prime_UTR
TGGGAAAACCTATTATCAGT+GGG 0.604807 8_1:+89000529 Msa1224210:CDS
AGAACTTCCCTCCATAGTAA+CGG 0.605346 8_1:-89000490 None:intergenic
CACGAGCAGCAATTCTCAAG+TGG 0.605488 8_1:-89001400 None:intergenic
CTGGGAAAACCTATTATCAG+TGG 0.606836 8_1:+89000528 Msa1224210:CDS
AAGGAAAAGTAGCAGCAGGA+AGG 0.607126 8_1:+89001912 Msa1224210:three_prime_UTR
CACTAGAACAACCGTAAAAG+GGG 0.608876 8_1:-89002201 None:intergenic
ACAATTGCATTGACTGCACA+AGG 0.614395 8_1:-89002097 None:intergenic
GTGGAGTTGTCACTTCTAAG+GGG 0.614783 8_1:+89001219 Msa1224210:CDS
TCGAAATGCTGATTTGATCA+GGG 0.621010 8_1:+89001484 Msa1224210:three_prime_UTR
TCAAAATGAATAATACTACA+AGG 0.628367 8_1:+88999473 Msa1224210:five_prime_UTR
GGAACTGAACTTGTCAAGGA+GGG 0.640908 8_1:+89000591 Msa1224210:CDS
GTGACTCAAGCAGTAAGACT+CGG 0.647491 8_1:+89001108 Msa1224210:CDS
TGGATGGTAACAAACTGATG+GGG 0.665765 8_1:+89001817 Msa1224210:three_prime_UTR
TGGAACTGAACTTGTCAAGG+AGG 0.668234 8_1:+89000590 Msa1224210:CDS
TGGAGTTGTCACTTCTAAGG+GGG 0.669080 8_1:+89001220 Msa1224210:CDS
GGATGGTAACAAACTGATGG+GGG 0.675199 8_1:+89001818 Msa1224210:three_prime_UTR
TTTGGTAAAACTGATGTGTG+TGG 0.689711 8_1:+89002020 Msa1224210:three_prime_UTR
AGGATAAATTCAAATCCTGG+AGG 0.721340 8_1:+89001026 Msa1224210:CDS
AATGTTACCGAAAAGATGCG+CGG 0.734112 8_1:-89000024 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATCAATTAAAACAAATAAT+TGG - chr8_1:89000191-89000210 None:intergenic 10.0%
!!! TTTTTTGTTAAAATATGATA+TGG + chr8_1:89001753-89001772 Msa1224210:intron 10.0%
!! TTTCATCAAATAAATGAAAT+TGG - chr8_1:88999431-88999450 None:intergenic 15.0%
!!! ATTTTTTAGTCTCTTAAATT+AGG + chr8_1:89000312-89000331 Msa1224210:intron 15.0%
!!! TTTATAATTTTATACAGGTT+AGG + chr8_1:89000970-89000989 Msa1224210:intron 15.0%
!! AAGCAAAATAAATTAAATGA+AGG - chr8_1:89001542-89001561 None:intergenic 15.0%
!!! TTTTTTCTATAGATTAAACA+TGG + chr8_1:89001576-89001595 Msa1224210:intron 15.0%
!! AAATTATGAAAACTTTCATA+GGG - chr8_1:89001731-89001750 None:intergenic 15.0%
!! AAAATTATGAAAACTTTCAT+AGG - chr8_1:89001732-89001751 None:intergenic 15.0%
!! TCAAAATGAATAATACTACA+AGG + chr8_1:88999473-88999492 Msa1224210:five_prime_UTR 20.0%
!! TATATATGTAAGTAACTACT+TGG - chr8_1:88999555-88999574 None:intergenic 20.0%
!! AGGATTCATGAAAATAAAAA+CGG - chr8_1:88999848-88999867 None:intergenic 20.0%
!! TAGATTATACTCAAAGATTA+TGG - chr8_1:89000449-89000468 None:intergenic 20.0%
!! CTATGTATTTGATGTAATTT+AGG + chr8_1:89000466-89000485 Msa1224210:intron 20.0%
!! CAACATTATCTATTCAATTA+AGG - chr8_1:89000928-89000947 None:intergenic 20.0%
!! TTGAATCTTCTTAATACAAT+AGG + chr8_1:89001513-89001532 Msa1224210:intron 20.0%
! TACAAAACAGAGAAACAAAA+AGG - chr8_1:88999380-88999399 None:intergenic 25.0%
! GATGATTAATGTTATCGTAA+AGG - chr8_1:88999767-88999786 None:intergenic 25.0%
! TCGTTGTTAATCAATTAAAG+TGG - chr8_1:89000277-89000296 None:intergenic 25.0%
! TATCAACAATCTTGAACAAA+AGG - chr8_1:89000368-89000387 None:intergenic 25.0%
! AAAACTCAAAACTACTGTTA+TGG + chr8_1:89000566-89000585 Msa1224210:CDS 25.0%
!!! TTATTTCTGAAGTTAATAGC+AGG - chr8_1:89000712-89000731 None:intergenic 25.0%
!!! CTATTGAATTGAATTTTGAC+TGG + chr8_1:89000818-89000837 Msa1224210:CDS 25.0%
!!! ATAGGGTATTTTTTTACCAT+GGG - chr8_1:89001714-89001733 None:intergenic 25.0%
!! TGAAATTTTACAAGAGAAGA+AGG + chr8_1:89001869-89001888 Msa1224210:three_prime_UTR 25.0%
AGAAACAAAAAGGCAAAGAA+AGG - chr8_1:88999370-88999389 None:intergenic 30.0%
AATATGAATGTTGAAGGAGA+GGG - chr8_1:88999590-88999609 None:intergenic 30.0%
TCTACGAATATGAATGTTGA+AGG - chr8_1:88999596-88999615 None:intergenic 30.0%
AAGGATTCATGAAAACGAAA+AGG - chr8_1:88999708-88999727 None:intergenic 30.0%
TTAGGCATTCTTCATCATTA+CGG + chr8_1:88999902-88999921 Msa1224210:intron 30.0%
!!! CAGTAGTTTTGAGTTTTCTT+TGG - chr8_1:89000563-89000582 None:intergenic 30.0%
!!! TTGAATTGAATTTTGACTGG+AGG + chr8_1:89000821-89000840 Msa1224210:CDS 30.0%
! TATTTTACCTTAAGCATCTC+AGG - chr8_1:89000895-89000914 None:intergenic 30.0%
! TTTTATACAGGTTAGGTATC+TGG + chr8_1:89000977-89000996 Msa1224210:intron 30.0%
CTCACTTGTTCATCTAAATA+TGG + chr8_1:89001282-89001301 Msa1224210:exon 30.0%
TAGTTAAAAATCCCAAGGAT+TGG + chr8_1:89001357-89001376 Msa1224210:three_prime_UTR 30.0%
! AATAAGCCAAACTTGTTTGT+TGG + chr8_1:89001645-89001664 Msa1224210:three_prime_UTR 30.0%
GTCATCAGAATTATTCATAG+TGG - chr8_1:89001679-89001698 None:intergenic 30.0%
!!! TAGGGTATTTTTTTACCATG+GGG - chr8_1:89001713-89001732 None:intergenic 30.0%
!!! CATAGGGTATTTTTTTACCA+TGG - chr8_1:89001715-89001734 None:intergenic 30.0%
!!! TTTTTTGTTGCAACAGCTAA+AGG + chr8_1:89001788-89001807 Msa1224210:intron 30.0%
TCAACTTTACTGTTTCAATC+TGG + chr8_1:89001964-89001983 Msa1224210:three_prime_UTR 30.0%
! CAAACTCTTCGTGATAATTT+TGG + chr8_1:89002002-89002021 Msa1224210:three_prime_UTR 30.0%
TACAGTACAAATCAGTTACT+TGG - chr8_1:89002139-89002158 None:intergenic 30.0%
GAATATGAATGTTGAAGGAG+AGG - chr8_1:88999591-88999610 None:intergenic 35.0%
! TCTCAACGTTTTCATCTTTC+AGG + chr8_1:88999660-88999679 Msa1224210:five_prime_UTR 35.0%
CATCAACAACAACAACAACA+AGG - chr8_1:88999727-88999746 None:intergenic 35.0%
GATGCTGATGAATCTTTCTT+CGG + chr8_1:88999811-88999830 Msa1224210:intron 35.0%
TTCACTGATGAATCTTCGTT+CGG + chr8_1:88999870-88999889 Msa1224210:intron 35.0%
! ATGAATCTTCGTTCGGTTTT+AGG + chr8_1:88999877-88999896 Msa1224210:intron 35.0%
!!! TTCGTTCGGTTTTAGGTTTT+AGG + chr8_1:88999884-88999903 Msa1224210:intron 35.0%
ATGAATCTTCGTTCGATTTC+AGG + chr8_1:88999981-89000000 Msa1224210:intron 35.0%
! GTAATTTAGGACCGTTACTA+TGG + chr8_1:89000479-89000498 Msa1224210:intron 35.0%
TGGGAAAACCTATTATCAGT+GGG + chr8_1:89000529-89000548 Msa1224210:CDS 35.0%
CTCAAAACTACTGTTATGGA+TGG + chr8_1:89000570-89000589 Msa1224210:CDS 35.0%
CAGAAATAAACCTGAAGATG+AGG + chr8_1:89000723-89000742 Msa1224210:intron 35.0%
AAATAAACCTGAAGATGAGG+AGG + chr8_1:89000726-89000745 Msa1224210:intron 35.0%
ATGAAAGAGATAAGGAAGTC+AGG + chr8_1:89001006-89001025 Msa1224210:CDS 35.0%
GTCAGGATAAATTCAAATCC+TGG + chr8_1:89001023-89001042 Msa1224210:CDS 35.0%
AGGATAAATTCAAATCCTGG+AGG + chr8_1:89001026-89001045 Msa1224210:CDS 35.0%
AAGCGTAGTTAAAAATCCCA+AGG + chr8_1:89001352-89001371 Msa1224210:three_prime_UTR 35.0%
TTAAAAATCCCAAGGATTGG+TGG + chr8_1:89001360-89001379 Msa1224210:three_prime_UTR 35.0%
TCGTGAATTCAATTGTAGCT+GGG + chr8_1:89001418-89001437 Msa1224210:three_prime_UTR 35.0%
!! ATCGAAATGCTGATTTGATC+AGG + chr8_1:89001483-89001502 Msa1224210:three_prime_UTR 35.0%
!! TCGAAATGCTGATTTGATCA+GGG + chr8_1:89001484-89001503 Msa1224210:three_prime_UTR 35.0%
AACATGGACAATTGATGCAA+AGG + chr8_1:89001592-89001611 Msa1224210:three_prime_UTR 35.0%
ACATGGACAATTGATGCAAA+GGG + chr8_1:89001593-89001612 Msa1224210:three_prime_UTR 35.0%
CTTTGACCAACAAACAAGTT+TGG - chr8_1:89001654-89001673 None:intergenic 35.0%
TTTACAAGAGAAGAAGGAGA+AGG + chr8_1:89001875-89001894 Msa1224210:three_prime_UTR 35.0%
TTTGGTAAAACTGATGTGTG+TGG + chr8_1:89002020-89002039 Msa1224210:three_prime_UTR 35.0%
TCACTAGAACAACCGTAAAA+GGG - chr8_1:89002205-89002224 None:intergenic 35.0%
TTCACTAGAACAACCGTAAA+AGG - chr8_1:89002206-89002225 None:intergenic 35.0%
! TGTTACAGATGTTGATGAGC+TGG - chr8_1:88999525-88999544 None:intergenic 40.0%
CGAAGATTCATCAGTGAACA+AGG - chr8_1:88999868-88999887 None:intergenic 40.0%
CGAAGATTCATCAGTGAACA+AGG - chr8_1:88999868-88999887 None:intergenic 40.0%
AATGTTACCGAAAAGATGCG+CGG - chr8_1:89000027-89000046 None:intergenic 40.0%
! ATTTAGGACCGTTACTATGG+AGG + chr8_1:89000482-89000501 Msa1224210:intron 40.0%
! TTTAGGACCGTTACTATGGA+GGG + chr8_1:89000483-89000502 Msa1224210:intron 40.0%
AGAACTTCCCTCCATAGTAA+CGG - chr8_1:89000493-89000512 None:intergenic 40.0%
! ACTATGGAGGGAAGTTCTAA+AGG + chr8_1:89000495-89000514 Msa1224210:exon 40.0%
! TTCTAAAGGTGATCATGTCC+TGG + chr8_1:89000509-89000528 Msa1224210:CDS 40.0%
TCTAAAGGTGATCATGTCCT+GGG + chr8_1:89000510-89000529 Msa1224210:CDS 40.0%
! CACTGATAATAGGTTTTCCC+AGG - chr8_1:89000530-89000549 None:intergenic 40.0%
CTGGGAAAACCTATTATCAG+TGG + chr8_1:89000528-89000547 Msa1224210:CDS 40.0%
CTACGTTTCCCACTGATAAT+AGG - chr8_1:89000540-89000559 None:intergenic 40.0%
AATGACACCTGAGATGCTTA+AGG + chr8_1:89000885-89000904 Msa1224210:CDS 40.0%
GGAGATGGATGAAAGAGATA+AGG + chr8_1:89000998-89001017 Msa1224210:CDS 40.0%
! TCAAGTGCATCCTCTTTTCT+AGG - chr8_1:89001053-89001072 None:intergenic 40.0%
GATGCACTTGAGATTTCTGA+TGG + chr8_1:89001062-89001081 Msa1224210:CDS 40.0%
! TAGTGGAGTTGTCACTTCTA+AGG + chr8_1:89001217-89001236 Msa1224210:CDS 40.0%
! AGTGGAGTTGTCACTTCTAA+GGG + chr8_1:89001218-89001237 Msa1224210:CDS 40.0%
CTCGTGAATTCAATTGTAGC+TGG + chr8_1:89001417-89001436 Msa1224210:three_prime_UTR 40.0%
CTGGATGGTAACAAACTGAT+GGG + chr8_1:89001816-89001835 Msa1224210:three_prime_UTR 40.0%
TGGATGGTAACAAACTGATG+GGG + chr8_1:89001817-89001836 Msa1224210:three_prime_UTR 40.0%
GAAGGAGAAGGAGAAAGAAA+AGG + chr8_1:89001893-89001912 Msa1224210:three_prime_UTR 40.0%
AGAAAAGGAAAAGTAGCAGC+AGG + chr8_1:89001908-89001927 Msa1224210:three_prime_UTR 40.0%
!! ACAATTGCATTGACTGCACA+AGG - chr8_1:89002100-89002119 None:intergenic 40.0%
CCGTAAAAGGGGTCAAATTT+GGG - chr8_1:89002193-89002212 None:intergenic 40.0%
! CCCAAATTTGACCCCTTTTA+CGG + chr8_1:89002190-89002209 Msa1224210:three_prime_UTR 40.0%
ACCGTAAAAGGGGTCAAATT+TGG - chr8_1:89002194-89002213 None:intergenic 40.0%
CACTAGAACAACCGTAAAAG+GGG - chr8_1:89002204-89002223 None:intergenic 40.0%
AAGAGAACTCAGACTCAGCT+GGG - chr8_1:88999404-88999423 None:intergenic 45.0%
AAAGAGAACTCAGACTCAGC+TGG - chr8_1:88999405-88999424 None:intergenic 45.0%
CAATTCCGATTGAAGCTTGC+CGG + chr8_1:88999497-88999516 Msa1224210:five_prime_UTR 45.0%
CTTTCTCTCATCGCTCTCAA+CGG + chr8_1:88999628-88999647 Msa1224210:five_prime_UTR 45.0%
! CATCTTTCCGCGCATCTTTT+CGG + chr8_1:89000017-89000036 Msa1224210:five_prime_UTR 45.0%
GGATGGAACTGAACTTGTCA+AGG + chr8_1:89000587-89000606 Msa1224210:CDS 45.0%
TGGAACTGAACTTGTCAAGG+AGG + chr8_1:89000590-89000609 Msa1224210:CDS 45.0%
GGAACTGAACTTGTCAAGGA+GGG + chr8_1:89000591-89000610 Msa1224210:CDS 45.0%
TGATCCTTTCACTGAAGAGC+TGG + chr8_1:89000774-89000793 Msa1224210:CDS 45.0%
ACAGGTTAGGTATCTGGAGA+TGG + chr8_1:89000983-89001002 Msa1224210:intron 45.0%
GTGACTCAAGCAGTAAGACT+CGG + chr8_1:89001108-89001127 Msa1224210:CDS 45.0%
AAGAGTAGTCTCCTCCAAGA+AGG + chr8_1:89001166-89001185 Msa1224210:CDS 45.0%
AGAGTAGTCTCCTCCAAGAA+GGG + chr8_1:89001167-89001186 Msa1224210:CDS 45.0%
AAGTGGAGTTGTCACTCCTA+AGG + chr8_1:89001190-89001209 Msa1224210:CDS 45.0%
AGTGGAGTTGTCACTCCTAA+GGG + chr8_1:89001191-89001210 Msa1224210:CDS 45.0%
! GTGGAGTTGTCACTTCTAAG+GGG + chr8_1:89001219-89001238 Msa1224210:CDS 45.0%
! TGGAGTTGTCACTTCTAAGG+GGG + chr8_1:89001220-89001239 Msa1224210:CDS 45.0%
GAATTCAATTGTAGCTGGGC+AGG + chr8_1:89001422-89001441 Msa1224210:three_prime_UTR 45.0%
GCTGGATGGTAACAAACTGA+TGG + chr8_1:89001815-89001834 Msa1224210:three_prime_UTR 45.0%
GGATGGTAACAAACTGATGG+GGG + chr8_1:89001818-89001837 Msa1224210:three_prime_UTR 45.0%
AGAGAAGAAGGAGAAGGAGA+AGG + chr8_1:89001881-89001900 Msa1224210:three_prime_UTR 45.0%
AAGGAAAAGTAGCAGCAGGA+AGG + chr8_1:89001912-89001931 Msa1224210:three_prime_UTR 45.0%
!!! AATTATTTATAATTTTATAC+AGG + chr8_1:89000965-89000984 Msa1224210:intron 5.0%
CGGCAAGCTTCAATCGGAAT+TGG - chr8_1:88999499-88999518 None:intergenic 50.0%
! AGATGTTGATGAGCTGGCAC+CGG - chr8_1:88999519-88999538 None:intergenic 50.0%
GAACTTGTCAAGGAGGGAAG+TGG + chr8_1:89000597-89000616 Msa1224210:CDS 50.0%
TCAAGGAGGGAAGTGGTAGT+AGG + chr8_1:89000604-89000623 Msa1224210:CDS 50.0%
AGGGAAGTGGTAGTAGGCAA+GGG + chr8_1:89000610-89000629 Msa1224210:CDS 50.0%
GCAAGGGAAGTCACATATCC+AGG + chr8_1:89000626-89000645 Msa1224210:CDS 50.0%
AGCTCCAGCTCTTCAGTGAA+AGG - chr8_1:89000781-89000800 None:intergenic 50.0%
TCCTGGAGGTCCTAGAAAAG+AGG + chr8_1:89001040-89001059 Msa1224210:CDS 50.0%
!!! TCCTCTTTTCTAGGACCTCC+AGG - chr8_1:89001044-89001063 None:intergenic 50.0%
AACTCCACTTCCCTTCTTGG+AGG - chr8_1:89001180-89001199 None:intergenic 50.0%
GACAACTCCACTTCCCTTCT+TGG - chr8_1:89001183-89001202 None:intergenic 50.0%
ACAACTCCACTAGCTCCCTT+AGG - chr8_1:89001209-89001228 None:intergenic 50.0%
! TGTCACTTCTAAGGGGGCTA+AGG + chr8_1:89001226-89001245 Msa1224210:CDS 50.0%
! GTCACTTCTAAGGGGGCTAA+GGG + chr8_1:89001227-89001246 Msa1224210:CDS 50.0%
!! TCCCAAGGATTGGTGGACTA+AGG + chr8_1:89001367-89001386 Msa1224210:three_prime_UTR 50.0%
GCCTTAGTCCACCAATCCTT+GGG - chr8_1:89001371-89001390 None:intergenic 50.0%
AGCCTTAGTCCACCAATCCT+TGG - chr8_1:89001372-89001391 None:intergenic 50.0%
CACGAGCAGCAATTCTCAAG+TGG - chr8_1:89001403-89001422 None:intergenic 50.0%
ATGCAAAGGGACGCCAAGTT+GGG + chr8_1:89001606-89001625 Msa1224210:three_prime_UTR 50.0%
GCAACAGCTAAAGGCTTTGC+TGG + chr8_1:89001797-89001816 Msa1224210:intron 50.0%
CAGCTAAAGGCTTTGCTGGA+TGG + chr8_1:89001801-89001820 Msa1224210:intron 50.0%
!! TGGCACCGGCAAGCTTCAAT+CGG - chr8_1:88999505-88999524 None:intergenic 55.0%
GAGGGAAGTGGTAGTAGGCA+AGG + chr8_1:89000609-89000628 Msa1224210:CDS 55.0%
CTGCATTGTCTGCAGTGTCC+TGG - chr8_1:89000647-89000666 None:intergenic 55.0%
CAGGACACTGCAGACAATGC+AGG + chr8_1:89000645-89000664 Msa1224210:CDS 55.0%
CACAGCTCCTCCTCATCTTC+AGG - chr8_1:89000736-89000755 None:intergenic 55.0%
GCTGGAGCTGCAACTTAGAG+AGG + chr8_1:89000792-89000811 Msa1224210:CDS 55.0%
GTCTCCTCCAAGAAGGGAAG+TGG + chr8_1:89001173-89001192 Msa1224210:CDS 55.0%
GTCACTCCTAAGGGAGCTAG+TGG + chr8_1:89001200-89001219 Msa1224210:CDS 55.0%
AAGGGAGCTGGAGGCAACAA+AGG + chr8_1:89001245-89001264 Msa1224210:CDS 55.0%
GATGCAAAGGGACGCCAAGT+TGG + chr8_1:89001605-89001624 Msa1224210:three_prime_UTR 55.0%
! AAGGGACGCCAAGTTGGGAA+AGG + chr8_1:89001611-89001630 Msa1224210:three_prime_UTR 55.0%
! AGGGACGCCAAGTTGGGAAA+GGG + chr8_1:89001612-89001631 Msa1224210:three_prime_UTR 55.0%
GTCAGACCCCTTTCCCAACT+TGG - chr8_1:89001622-89001641 None:intergenic 55.0%
TGACTCAGCTGTTGACCCCA+TGG + chr8_1:89001695-89001714 Msa1224210:three_prime_UTR 55.0%
TCTAAGGGGGCTAAGGGAGC+TGG + chr8_1:89001233-89001252 Msa1224210:CDS 60.0%
! GGGACGCCAAGTTGGGAAAG+GGG + chr8_1:89001613-89001632 Msa1224210:three_prime_UTR 60.0%
AAGGGGGCTAAGGGAGCTGG+AGG + chr8_1:89001236-89001255 Msa1224210:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 88999342 89002245 88999342 ID=Msa1224210;Name=Msa1224210
chr8_1 mRNA 88999342 89002245 88999342 ID=Msa1224210-mRNA-1;Parent=Msa1224210;Name=Msa1224210-mRNA-1;_AED=0.09;_eAED=0.09;_QI=386|1|1|1|0.66|0.71|7|778|215
chr8_1 exon 88999342 88999681 88999342 ID=Msa1224210-mRNA-1:exon:32349;Parent=Msa1224210-mRNA-1
chr8_1 exon 89000003 89000038 89000003 ID=Msa1224210-mRNA-1:exon:32350;Parent=Msa1224210-mRNA-1
chr8_1 exon 89000488 89000666 89000488 ID=Msa1224210-mRNA-1:exon:32351;Parent=Msa1224210-mRNA-1
chr8_1 exon 89000740 89000906 89000740 ID=Msa1224210-mRNA-1:exon:32352;Parent=Msa1224210-mRNA-1
chr8_1 exon 89000987 89001505 89000987 ID=Msa1224210-mRNA-1:exon:32353;Parent=Msa1224210-mRNA-1
chr8_1 exon 89001588 89001716 89001588 ID=Msa1224210-mRNA-1:exon:32354;Parent=Msa1224210-mRNA-1
chr8_1 exon 89001804 89002245 89001804 ID=Msa1224210-mRNA-1:exon:32355;Parent=Msa1224210-mRNA-1
chr8_1 five_prime_UTR 88999342 88999681 88999342 ID=Msa1224210-mRNA-1:five_prime_utr;Parent=Msa1224210-mRNA-1
chr8_1 five_prime_UTR 89000003 89000038 89000003 ID=Msa1224210-mRNA-1:five_prime_utr;Parent=Msa1224210-mRNA-1
chr8_1 five_prime_UTR 89000488 89000497 89000488 ID=Msa1224210-mRNA-1:five_prime_utr;Parent=Msa1224210-mRNA-1
chr8_1 CDS 89000498 89000666 89000498 ID=Msa1224210-mRNA-1:cds;Parent=Msa1224210-mRNA-1
chr8_1 CDS 89000740 89000906 89000740 ID=Msa1224210-mRNA-1:cds;Parent=Msa1224210-mRNA-1
chr8_1 CDS 89000987 89001298 89000987 ID=Msa1224210-mRNA-1:cds;Parent=Msa1224210-mRNA-1
chr8_1 three_prime_UTR 89001299 89001505 89001299 ID=Msa1224210-mRNA-1:three_prime_utr;Parent=Msa1224210-mRNA-1
chr8_1 three_prime_UTR 89001588 89001716 89001588 ID=Msa1224210-mRNA-1:three_prime_utr;Parent=Msa1224210-mRNA-1
chr8_1 three_prime_UTR 89001804 89002245 89001804 ID=Msa1224210-mRNA-1:three_prime_utr;Parent=Msa1224210-mRNA-1
Gene Sequence

>Msa1224210

ATGGAGGGAAGTTCTAAAGGTGATCATGTCCTGGGAAAACCTATTATCAGTGGGAAACGTAGCCAAAGAAAACTCAAAACTACTGTTATGGATGGAACTGAACTTGTCAAGGAGGGAAGTGGTAGTAGGCAAGGGAAGTCACATATCCAGGACACTGCAGACAATGCAGATGAGGAGGAGCTGTGCGATTCATTGCTTTTTGTTGATCCTTTCACTGAAGAGCTGGAGCTGCAACTTAGAGAGGCTGCTATTGAATTGAATTTTGACTGGAGGAAGATCAAGAGATCTAAAAAATATGCTAATATTTTTAAAGAAATGACACCTGAGATGCTTAAGGTTAGGTATCTGGAGATGGATGAAAGAGATAAGGAAGTCAGGATAAATTCAAATCCTGGAGGTCCTAGAAAAGAGGATGCACTTGAGATTTCTGATGGTAGCTCAAGCAGAAAGACTGATAGTGACTCAAGCAGTAAGACTCGGACAGATGATGATTCTATTCAGCTGAGAAGAAGTGCAAGAGTAGTCTCCTCCAAGAAGGGAAGTGGAGTTGTCACTCCTAAGGGAGCTAGTGGAGTTGTCACTTCTAAGGGGGCTAAGGGAGCTGGAGGCAACAAAGGTTCTCTTACATTCACTCACTTGTTCATCTAA
Protein sequence

>Msa1224210

MEGSSKGDHVLGKPIISGKRSQRKLKTTVMDGTELVKEGSGSRQGKSHIQDTADNADEEELCDSLLFVDPFTEELELQLREAAIELNFDWRKIKRSKKYANIFKEMTPEMLKVRYLEMDERDKEVRINSNPGGPRKEDALEISDGSSSRKTDSDSSSKTRTDDDSIQLRRSARVVSSKKGSGVVTPKGASGVVTSKGAKGAGGNKGSLTFTHLFI*