AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0696530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0696530 MtrunA17_Chr4g0048921 91.608 572 46 2 1 572 1 570 0.0 1070
Msa0696530 MtrunA17_Chr4g0048911 48.824 510 247 8 66 569 55 556 9.64e-168 488
Msa0696530 MtrunA17_Chr3g0108991 44.016 493 266 6 82 567 23 512 1.49e-142 422
Msa0696530 MtrunA17_Chr3g0108951 44.016 493 266 6 82 567 23 512 1.49e-142 422
Msa0696530 MtrunA17_Chr2g0280581 44.242 495 263 9 82 567 27 517 8.00e-142 420
Msa0696530 MtrunA17_Chr3g0099031 44.334 503 264 9 75 567 16 512 8.27e-142 420
Msa0696530 MtrunA17_Chr3g0108971 43.878 490 265 6 85 567 1 487 2.42e-141 418
Msa0696530 MtrunA17_Chr3g0105691 44.016 493 266 6 82 567 56 545 3.06e-141 420
Msa0696530 MtrunA17_Chr3g0108941 43.117 494 270 6 82 567 23 513 1.49e-140 417
Msa0696530 MtrunA17_Chr3g0109031 43.002 493 271 6 82 567 23 512 4.73e-140 416
Msa0696530 MtrunA17_Chr6g0477111 43.469 490 267 6 85 567 1 487 1.12e-139 414
Msa0696530 MtrunA17_Chr3g0109011 43.265 490 268 6 85 567 1 487 1.18e-139 414
Msa0696530 MtrunA17_Chr3g0109021 43.205 493 270 6 82 567 23 512 1.19e-139 415
Msa0696530 MtrunA17_Chr3g0109001 42.915 494 271 6 82 567 23 513 5.06e-139 413
Msa0696530 MtrunA17_Chr3g0108981 42.770 491 270 6 85 567 1 488 4.00e-137 407
Msa0696530 MtrunA17_Chr3g0108961 42.770 491 270 6 85 567 1 488 4.00e-137 407
Msa0696530 MtrunA17_Chr6g0477121 45.131 421 224 5 143 559 5 422 5.55e-125 374
Msa0696530 MtrunA17_Chr2g0323131 36.965 514 299 7 65 568 62 560 2.51e-114 351
Msa0696530 MtrunA17_Chr2g0323151 37.759 482 291 4 87 568 3 475 2.38e-110 338
Msa0696530 MtrunA17_Chr4g0042111 35.394 469 295 4 90 555 79 542 3.41e-95 301
Msa0696530 MtrunA17_Chr3g0082691 33.205 518 333 5 63 571 38 551 6.04e-94 298
Msa0696530 MtrunA17_Chr2g0310331 34.766 512 321 7 66 571 80 584 9.76e-94 298
Msa0696530 MtrunA17_Chr4g0023911 34.369 483 310 4 78 557 85 563 9.38e-92 293
Msa0696530 MtrunA17_Chr7g0236991 32.866 499 325 4 63 555 39 533 1.79e-90 289
Msa0696530 MtrunA17_Chr7g0237001 32.665 499 326 4 63 555 39 533 9.77e-90 287
Msa0696530 MtrunA17_Chr7g0237041 32.665 499 326 4 63 555 39 533 9.77e-90 287
Msa0696530 MtrunA17_Chr7g0237081 32.665 499 326 4 63 555 39 533 9.77e-90 287
Msa0696530 MtrunA17_Chr7g0237051 32.665 499 326 4 63 555 39 533 9.77e-90 287
Msa0696530 MtrunA17_Chr7g0236961 33.403 476 311 3 82 555 7 478 8.17e-89 283
Msa0696530 MtrunA17_Chr5g0431651 32.039 515 336 5 67 571 40 550 3.86e-88 283
Msa0696530 MtrunA17_Chr7g0237011 33.193 476 312 3 82 555 7 478 6.64e-88 280
Msa0696530 MtrunA17_Chr6g0465441 31.660 518 340 8 63 571 39 551 7.24e-88 282
Msa0696530 MtrunA17_Chr3g0105071 31.792 519 341 5 63 572 42 556 5.64e-87 280
Msa0696530 MtrunA17_Chr5g0425371 32.692 520 332 8 63 571 39 551 1.48e-86 279
Msa0696530 MtrunA17_Chr6g0451711 31.407 519 341 6 63 571 42 555 1.88e-86 278
Msa0696530 MtrunA17_Chr4g0024691 31.115 511 340 5 69 571 53 559 1.48e-85 276
Msa0696530 MtrunA17_Chr5g0431571 31.712 514 338 5 67 571 38 547 1.32e-84 273
Msa0696530 MtrunA17_Chr8g0357411 33.266 496 316 9 82 571 101 587 7.80e-84 273
Msa0696530 MtrunA17_Chr2g0319051 32.617 512 325 9 71 571 10 512 1.17e-81 265
Msa0696530 MtrunA17_Chr8g0336181 32.000 525 328 11 65 571 41 554 4.51e-78 256
Msa0696530 MtrunA17_Chr6g0466051 29.207 517 354 5 63 571 39 551 5.57e-78 256
Msa0696530 MtrunA17_Chr6g0465851 29.014 517 355 5 63 571 4 516 1.63e-77 254
Msa0696530 MtrunA17_Chr6g0465911 29.014 517 355 5 63 571 39 551 3.89e-77 254
Msa0696530 MtrunA17_Chr2g0310401 34.450 418 267 5 145 560 5 417 5.10e-75 245
Msa0696530 MtrunA17_Chr2g0319041 29.714 525 345 11 63 571 39 555 3.52e-74 246
Msa0696530 MtrunA17_Chr7g0236971 32.987 385 254 2 171 555 1 381 1.10e-68 227
Msa0696530 MtrunA17_Chr4g0008621 33.850 387 232 8 63 433 47 425 5.59e-64 216
Msa0696530 MtrunA17_Chr7g0236951 37.542 301 185 2 82 380 7 306 1.10e-61 206
Msa0696530 MtrunA17_Chr7g0237031 37.209 301 186 2 82 380 7 306 4.92e-61 204
Msa0696530 MtrunA17_Chr6g0465931 34.700 317 200 3 63 373 4 319 1.70e-56 193
Msa0696530 MtrunA17_Chr6g0466111 34.385 317 201 3 63 373 4 319 1.07e-55 191
Msa0696530 MtrunA17_Chr6g0465981 34.385 317 201 3 63 373 39 354 3.20e-55 191
Msa0696530 MtrunA17_Chr6g0465871 34.385 317 201 3 63 373 39 354 3.20e-55 191
Msa0696530 MtrunA17_Chr6g0466081 34.385 317 201 3 63 373 39 354 3.20e-55 191
Msa0696530 MtrunA17_Chr8g0357231 35.878 262 160 5 313 571 1 257 5.86e-50 173
Msa0696530 MtrunA17_Chr7g0217361 32.979 282 184 3 268 548 6 283 2.56e-46 165
Msa0696530 MtrunA17_Chr2g0319071 28.049 492 296 15 88 571 46 487 5.02e-44 163
Msa0696530 MtrunA17_Chr5g0445511 37.156 218 128 4 167 380 2 214 1.14e-41 150
Msa0696530 MtrunA17_Chr3g0101681 24.760 521 340 13 88 572 277 781 4.99e-39 152
Msa0696530 MtrunA17_Chr2g0310121 23.483 511 359 10 88 572 276 780 2.49e-36 144
Msa0696530 MtrunA17_Chr2g0319081 28.788 330 211 9 246 571 116 425 1.20e-35 139
Msa0696530 MtrunA17_Chr7g0237061 28.395 243 147 2 313 555 2 217 2.58e-30 119
Msa0696530 MtrunA17_Chr7g0237071 35.204 196 125 1 82 275 7 202 2.13e-28 113
Msa0696530 MtrunA17_Chr6g0466031 24.904 261 190 3 313 571 2 258 3.34e-28 113
Msa0696530 MtrunA17_Chr2g0281651 26.800 250 174 5 250 497 45 287 1.63e-21 97.1
Msa0696530 MtrunA17_Chr7g0218001 27.376 263 159 8 88 320 347 607 5.16e-20 94.4
Msa0696530 MtrunA17_Chr8g0357221 32.812 192 126 2 82 272 101 290 8.19e-20 90.1
Msa0696530 MtrunA17_Chr6g0466021 35.971 139 83 2 63 195 39 177 1.79e-19 87.0
Msa0696530 MtrunA17_Chr5g0445501 28.324 173 119 2 401 571 1 170 6.18e-19 84.7
Msa0696530 MtrunA17_Chr7g0259881 25.185 270 172 8 88 328 230 498 7.23e-19 90.5
Msa0696530 MtrunA17_Chr7g0217351 30.476 210 134 6 70 273 70 273 9.49e-19 87.0
Msa0696530 MtrunA17_Chr7g0237021 27.742 155 109 1 401 555 1 152 1.69e-17 80.5
Msa0696530 MtrunA17_Chr7g0236941 27.742 155 109 1 401 555 1 152 1.69e-17 80.5
Msa0696530 MtrunA17_Chr7g0233861 28.986 138 89 1 208 345 30 158 2.49e-15 74.3
Msa0696530 MtrunA17_Chr6g0465881 22.543 173 129 2 401 571 1 170 2.06e-13 68.6
Msa0696530 MtrunA17_Chr6g0465941 22.543 173 129 2 401 571 1 170 2.59e-13 68.6
Msa0696530 MtrunA17_Chr6g0466121 22.543 173 129 2 401 571 1 170 2.59e-13 68.6
Msa0696530 MtrunA17_Chr6g0465991 22.543 173 129 2 401 571 1 170 2.59e-13 68.6
Msa0696530 MtrunA17_Chr6g0466091 22.543 173 129 2 401 571 1 170 2.59e-13 68.6
Msa0696530 MtrunA17_Chr7g0218041 26.250 240 150 7 133 347 1 238 8.02e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0696530 AT1G61680.1 45.709 571 289 10 6 567 11 569 3.09e-160 469
Msa0696530 AT1G61680.2 45.709 571 282 11 6 567 11 562 1.53e-157 462
Msa0696530 AT4G16730.1 33.397 524 323 8 67 571 21 537 9.39e-93 295
Msa0696530 AT4G16730.2 33.333 498 309 7 90 571 1 491 4.48e-90 286
Msa0696530 AT4G16740.1 34.180 512 294 13 68 557 55 545 5.41e-86 278
Msa0696530 AT3G25820.1 31.472 591 358 13 4 571 26 592 3.11e-84 274
Msa0696530 AT3G25830.1 31.472 591 358 13 4 571 26 592 3.11e-84 274
Msa0696530 AT2G24210.1 30.944 572 352 14 18 571 42 588 3.43e-82 269
Msa0696530 AT3G25810.1 30.648 509 326 10 82 571 95 595 1.04e-76 254
Msa0696530 AT5G23960.1 28.599 514 359 4 63 571 35 545 4.69e-72 241
Msa0696530 AT5G23960.2 28.571 511 357 4 65 571 37 543 1.67e-71 239
Msa0696530 AT1G70080.1 28.429 503 342 10 81 571 113 609 9.10e-64 220
Msa0696530 AT3G14520.1 26.369 493 350 7 72 557 100 586 4.55e-56 199
Msa0696530 AT3G14490.1 26.984 504 352 7 64 557 85 582 2.24e-54 194
Msa0696530 AT3G25820.2 30.969 423 255 9 4 410 26 427 4.02e-54 190
Msa0696530 AT3G14540.1 25.631 515 360 8 60 557 75 583 7.14e-54 193
Msa0696530 AT4G16740.2 34.195 348 197 10 65 390 52 389 1.09e-52 185
Msa0696530 AT3G29410.2 27.521 476 327 8 90 557 15 480 1.33e-52 187
Msa0696530 AT3G29410.1 27.521 476 327 8 90 557 120 585 1.05e-51 187
Msa0696530 AT1G31950.3 25.738 474 341 5 90 557 121 589 3.98e-51 186
Msa0696530 AT1G31950.1 25.738 474 341 5 90 557 120 588 4.16e-51 186
Msa0696530 AT4G13300.1 25.919 517 367 6 67 571 40 552 2.03e-49 180
Msa0696530 AT3G14540.2 25.986 431 302 5 60 475 75 503 7.94e-49 177
Msa0696530 AT1G33750.1 24.606 508 370 6 69 568 96 598 5.74e-48 177
Msa0696530 AT5G44630.1 26.962 497 345 8 86 572 68 556 8.75e-48 175
Msa0696530 AT4G20230.1 25.382 524 374 8 59 569 82 601 1.73e-47 175
Msa0696530 AT4G15870.1 24.811 528 364 9 59 571 87 596 1.85e-47 175
Msa0696530 AT4G20230.2 25.382 524 374 8 59 569 94 613 2.16e-47 175
Msa0696530 AT4G13280.2 26.834 477 339 5 101 571 80 552 1.65e-46 172
Msa0696530 AT4G20200.1 25.859 495 359 4 82 571 111 602 5.79e-46 171
Msa0696530 AT1G31950.2 25.806 403 293 3 90 487 121 522 2.01e-45 168
Msa0696530 AT3G29190.1 24.213 508 373 4 59 557 79 583 2.19e-44 167
Msa0696530 AT1G66020.1 26.046 526 371 8 59 571 76 596 3.97e-42 160
Msa0696530 AT1G48800.1 25.940 532 365 14 57 569 81 602 4.29e-42 160
Msa0696530 AT4G16740.4 32.440 336 194 9 65 382 52 372 6.03e-42 155
Msa0696530 AT4G16740.3 32.440 336 194 9 65 382 52 372 6.03e-42 155
Msa0696530 AT4G20210.1 24.860 535 379 10 42 567 74 594 1.07e-40 156
Msa0696530 AT3G32030.1 23.414 457 343 4 90 543 119 571 2.49e-39 152
Msa0696530 AT2G23230.2 25.800 500 351 11 82 569 105 596 9.29e-39 150
Msa0696530 AT5G48110.1 29.358 327 222 6 247 571 18 337 1.23e-38 145
Msa0696530 AT4G13280.1 25.996 477 313 7 101 571 80 522 1.54e-38 149
Msa0696530 AT1G48820.1 23.909 527 348 11 53 569 77 560 1.71e-38 149
Msa0696530 AT3G25820.4 30.168 358 214 8 4 345 26 363 8.47e-38 144
Msa0696530 AT3G25820.3 30.168 358 214 8 4 345 26 363 8.47e-38 144
Msa0696530 AT2G23230.1 25.651 499 348 11 82 569 105 591 9.41e-38 147
Msa0696530 AT3G29110.1 24.381 525 381 8 59 571 75 595 2.02e-37 147
Msa0696530 AT1G66020.2 26.498 317 224 3 59 366 76 392 2.62e-29 120
Msa0696530 AT1G79460.1 23.619 525 338 14 88 571 275 777 1.87e-25 111
Msa0696530 AT1G61120.1 25.000 380 247 8 128 485 333 696 6.97e-23 103
Msa0696530 AT4G02780.1 28.571 266 158 9 88 323 332 595 7.83e-18 87.8

Find 142 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCATGCTTCTTATATATTT+TGG 0.063401 4_4:+66491373 None:intergenic
CTTCGACTTTGGGATGATTT+AGG 0.178417 4_4:-66490990 Msa0696530:CDS
ATGACCACATGTACCATTTA+AGG 0.184226 4_4:+66491713 None:intergenic
GGTATTGGGATTAATAATTT+TGG 0.184662 4_4:+66490056 None:intergenic
ATTACATGAAAACATGTTTC+AGG 0.215235 4_4:-66491427 Msa0696530:CDS
CTGCCACAATTCTTCGACTT+TGG 0.232538 4_4:-66491001 Msa0696530:CDS
CAAAGTGGTAATCAATATTT+AGG 0.236989 4_4:+66492751 None:intergenic
AGACCTTTGTGAATGCTTTA+TGG 0.251154 4_4:+66490196 None:intergenic
TGTTCACTGAAGTTGTTTCT+AGG 0.259492 4_4:-66491570 Msa0696530:intron
TATGAAAGAAGTTGCGAAAA+TGG 0.272045 4_4:-66492105 Msa0696530:CDS
TGCCACAATTCTTCGACTTT+GGG 0.273382 4_4:-66491000 Msa0696530:CDS
CATATTGGCTAATGGGTATT+GGG 0.275028 4_4:+66490042 None:intergenic
CCTTTCTAGTTGAAGCAAAA+TGG 0.291776 4_4:-66491187 Msa0696530:CDS
TATTCCTTCATATTGGCTAA+TGG 0.291833 4_4:+66490034 None:intergenic
GATGATAAGCACTCCCTTCC+TGG 0.293520 4_4:-66490132 Msa0696530:CDS
TAGCGACATATTGTCTTCTT+AGG 0.299306 4_4:+66492709 None:intergenic
ATTATGTAGATTAAAGATAT+TGG 0.303530 4_4:+66491637 None:intergenic
GAAGCAAAATGGTTTGGTTC+AGG 0.309836 4_4:-66491176 Msa0696530:CDS
TCTTTCGAAGAGAGTCTTTA+GGG 0.310663 4_4:+66491345 None:intergenic
CTAGAAACCAGCCCCTTAAA+TGG 0.310744 4_4:-66491726 Msa0696530:CDS
ATTGAGTTATTCCTTCATAT+TGG 0.328311 4_4:+66490027 None:intergenic
TTTAAGTATTCTTCAGCATT+TGG 0.333715 4_4:+66491146 None:intergenic
GTCAAATCCGCTAATATGTT+TGG 0.334467 4_4:-66489924 Msa0696530:three_prime_UTR
TTGTGAATGCTTTATGGAAA+TGG 0.335415 4_4:+66490202 None:intergenic
ATTCCTTCATATTGGCTAAT+GGG 0.336409 4_4:+66490035 None:intergenic
TGTGGCAGGGGCGGAAATAA+TGG 0.340825 4_4:+66491016 None:intergenic
TATGAAGGAATAACTCAATA+GGG 0.358662 4_4:-66490023 Msa0696530:three_prime_UTR
TCATATTGGCTAATGGGTAT+TGG 0.362384 4_4:+66490041 None:intergenic
ATATGAAGGAATAACTCAAT+AGG 0.382633 4_4:-66490024 Msa0696530:three_prime_UTR
CTAGTTGAAGCAAAATGGTT+TGG 0.387597 4_4:-66491182 Msa0696530:CDS
GTCTTTCGAAGAGAGTCTTT+AGG 0.392280 4_4:+66491344 None:intergenic
AGGGGCTGGTTTCTAGCATA+TGG 0.394011 4_4:+66491733 None:intergenic
TATAGCACAAATGCATGGTT+TGG 0.394113 4_4:-66492131 Msa0696530:CDS
TTCACAGCTAGAAATTTCTA+TGG 0.410766 4_4:-66492161 Msa0696530:CDS
TTCACGAAGCTAAGTTTGTA+AGG 0.412343 4_4:-66492223 Msa0696530:CDS
TGACCACATGTACCATTTAA+GGG 0.423595 4_4:+66491714 None:intergenic
GCTGAAGAATACTTAAAGAA+TGG 0.428187 4_4:-66491140 Msa0696530:CDS
TTTGTGAAAAGGATACTCTA+AGG 0.428411 4_4:+66492196 None:intergenic
TAAATGGTACATGTGGTCAT+TGG 0.428916 4_4:-66491710 Msa0696530:CDS
TGTAGATGGTGGAGAGAACT+TGG 0.441935 4_4:-66491772 Msa0696530:intron
CTGAAGAATACTTAAAGAAT+GGG 0.442910 4_4:-66491139 Msa0696530:CDS
ATTTAGCAGGAAGATCCTTA+AGG 0.443162 4_4:-66492267 Msa0696530:CDS
TTTGGGTAAAGGATTAACTA+AGG 0.443181 4_4:-66491069 Msa0696530:CDS
TTTGGCACGCCTTGTGCTGA+AGG 0.448254 4_4:-66489842 Msa0696530:three_prime_UTR
ATGAGACCGCACTTCACTTC+AGG 0.453303 4_4:-66492653 Msa0696530:CDS
GCTGCAAGGCTTACACCATC+AGG 0.453407 4_4:-66492072 Msa0696530:CDS
ATGGGATATTGACACAGATA+TGG 0.458966 4_4:-66491462 Msa0696530:CDS
CAAGTATGTCTTCCCCTGCC+AGG 0.463054 4_4:+66492303 None:intergenic
ATTCTTTCCTCAGATAAAGT+AGG 0.467948 4_4:+66491673 None:intergenic
TCAAATCCGCTAATATGTTT+GGG 0.468448 4_4:-66489923 Msa0696530:three_prime_UTR
CTCTGGAGTGCATATTGTGC+TGG 0.472322 4_4:-66491108 Msa0696530:CDS
TGTGAATGCTTTATGGAAAT+GGG 0.472761 4_4:+66490203 None:intergenic
TGAGAAAGTTGTGCAATTGT+TGG 0.473300 4_4:+66493083 None:intergenic
CACTCCCTTCCTGGGCTAGA+GGG 0.473788 4_4:-66490123 Msa0696530:CDS
GAAAATCAACAAGGAAATGA+TGG 0.476240 4_4:-66490354 Msa0696530:CDS
GCCTCGCATCTAAACCTGGC+AGG 0.477164 4_4:-66492317 Msa0696530:CDS
GCAATTGTTGGGACGATTTG+AGG 0.478213 4_4:+66493095 None:intergenic
TCCAAAATATATAAGAAGCA+TGG 0.481912 4_4:-66491374 Msa0696530:CDS
TTACATGAAAACATGTTTCA+GGG 0.487840 4_4:-66491426 Msa0696530:CDS
CTCTTCACATTGCTAACAAG+TGG 0.489348 4_4:-66493034 Msa0696530:CDS
GAGAAAGTTGTGCAATTGTT+GGG 0.490482 4_4:+66493084 None:intergenic
AAATGGTACATGTGGTCATT+GGG 0.490912 4_4:-66491709 Msa0696530:CDS
GTTCATTTGTTAGATCATAG+AGG 0.495017 4_4:+66491402 None:intergenic
AAGCAAAATGGTTTGGTTCA+GGG 0.495976 4_4:-66491175 Msa0696530:CDS
CAAAGTCGAAGAATTGTGGC+AGG 0.496180 4_4:+66491002 None:intergenic
CTCACTCAATCTTGCAAGGA+TGG 0.499444 4_4:-66490175 Msa0696530:CDS
GTGTCTAATGATGGACCAAC+CGG 0.500032 4_4:-66490319 Msa0696530:CDS
AAACTAGGTGAAAATATCAA+TGG 0.502965 4_4:-66492359 Msa0696530:CDS
ATGAAGCTGGAAAATTTAGC+AGG 0.503149 4_4:-66492280 Msa0696530:CDS
ACATGTACCATTTAAGGGGC+TGG 0.504728 4_4:+66491719 None:intergenic
GTTCAATTTCTTCTTCAAAG+TGG 0.514024 4_4:+66492736 None:intergenic
CCGCTAATATGTTTGGGTAT+CGG 0.515981 4_4:-66489917 Msa0696530:three_prime_UTR
AAGAATGGGATAGTAAGCTC+TGG 0.518549 4_4:-66491125 Msa0696530:CDS
GTTGGTGAAAATTCACTCAA+AGG 0.524144 4_4:-66492801 Msa0696530:CDS
CCAAAATATATAAGAAGCAT+GGG 0.524424 4_4:-66491373 Msa0696530:CDS
GCATTAATGTTGAAGAAGAA+AGG 0.527443 4_4:-66493010 Msa0696530:CDS
CCCTGCCAGGTTTAGATGCG+AGG 0.529393 4_4:+66492316 None:intergenic
ATCAATATTTAGGCGCTGCA+TGG 0.529573 4_4:+66492761 None:intergenic
TGAATAAAATATCCGATGCA+TGG 0.532850 4_4:-66490257 Msa0696530:CDS
TGAAGCCTCGCATCTAAACC+TGG 0.533102 4_4:-66492321 Msa0696530:CDS
ATGATAAGCACTCCCTTCCT+GGG 0.538683 4_4:-66490131 Msa0696530:CDS
GTATATTCATTCCTTTGCCA+TGG 0.540974 4_4:-66493153 Msa0696530:exon
CAAAGAGACACTCTTGGTTG+AGG 0.541785 4_4:+66490229 None:intergenic
TTGGCACGCCTTGTGCTGAA+GGG 0.544809 4_4:-66489841 Msa0696530:three_prime_UTR
TCATCATAACTATACATCAA+TGG 0.553674 4_4:+66490150 None:intergenic
ATACGTGAAGTGTCTAATGA+TGG 0.556244 4_4:-66490328 Msa0696530:CDS
CCGATACCCAAACATATTAG+CGG 0.557150 4_4:+66489917 None:intergenic
GCACTCCCTTCCTGGGCTAG+AGG 0.557886 4_4:-66490124 Msa0696530:CDS
CTTCTTGTACAATAATACTC+CGG 0.561255 4_4:+66490300 None:intergenic
CCTCGCATCTAAACCTGGCA+GGG 0.562204 4_4:-66492316 Msa0696530:CDS
GAAGACATACTTGATGAAGC+TGG 0.565437 4_4:-66492293 Msa0696530:CDS
ATCCCAAAGTCGAAGAATTG+TGG 0.568131 4_4:+66490998 None:intergenic
CTTTATCATAAAGCAAGGAA+TGG 0.568777 4_4:+66490094 None:intergenic
CAAGGTCACTTTGTTCCCGC+AGG 0.569400 4_4:-66492618 Msa0696530:intron
TTTGAGGTTTCAAAGAGGAA+AGG 0.569570 4_4:+66493111 None:intergenic
CTCGCATCTAAACCTGGCAG+GGG 0.571060 4_4:-66492315 Msa0696530:CDS
CAAATGCAGTGCCATGGCAA+AGG 0.571735 4_4:+66493142 None:intergenic
GAAAAGTTTGATCCAAAACT+AGG 0.573193 4_4:-66492374 Msa0696530:CDS
ATACCCATTAGCCAATATGA+AGG 0.575623 4_4:-66490038 Msa0696530:three_prime_UTR
CAGGGGCGGAAATAATGGAT+AGG 0.578243 4_4:+66491021 None:intergenic
TGGACATAACCCTCTAGCCC+AGG 0.581943 4_4:+66490114 None:intergenic
AAATTTCTAGCTGTGAACAT+TGG 0.584942 4_4:+66492167 None:intergenic
ATGGATTTCAGTTTGCTGCA+AGG 0.586908 4_4:-66492086 Msa0696530:CDS
ATTTATATAGCACAAATGCA+TGG 0.589084 4_4:-66492136 Msa0696530:CDS
TAAAGACTCTCTTCGAAAGA+CGG 0.589563 4_4:-66491342 Msa0696530:intron
AGGTCTCACTCAATCTTGCA+AGG 0.596435 4_4:-66490179 Msa0696530:CDS
AATATATAAGAAGCATGGGT+GGG 0.597265 4_4:-66491369 Msa0696530:CDS
GTGGTCATTGGGATGCCTCA+CGG 0.597557 4_4:-66491698 Msa0696530:CDS
TAAGGAAAATGTTCATACAA+TGG 0.597774 4_4:-66491051 Msa0696530:CDS
TCGAAGAATTGTGGCAGGGG+CGG 0.597807 4_4:+66491007 None:intergenic
AAGTCGAAGAATTGTGGCAG+GGG 0.600683 4_4:+66491004 None:intergenic
AAATATATAAGAAGCATGGG+TGG 0.602804 4_4:-66491370 Msa0696530:CDS
CATAACCCTCTAGCCCAGGA+AGG 0.603462 4_4:+66490118 None:intergenic
CACGGATCCTACTTTATCTG+AGG 0.604127 4_4:-66491680 Msa0696530:CDS
AGGGGCGGAAATAATGGATA+GGG 0.604700 4_4:+66491022 None:intergenic
GACCACATGTACCATTTAAG+GGG 0.604906 4_4:+66491715 None:intergenic
AATATACGTAAGTCAAGTAG+TGG 0.607241 4_4:+66493169 None:intergenic
TTGTGCTGAAGGGATTGCTG+TGG 0.608283 4_4:-66489831 Msa0696530:three_prime_UTR
GGGTATCAAAGAGACACTCT+TGG 0.612403 4_4:+66490223 None:intergenic
ACAGCAATCCCTTCAGCACA+AGG 0.613899 4_4:+66489833 None:intergenic
ATGAAGGAATAACTCAATAG+GGG 0.613993 4_4:-66490022 Msa0696530:three_prime_UTR
AAGCATGAACTTAGAAACGT+TGG 0.614659 4_4:-66492819 Msa0696530:CDS
CAATATGTCGCTAGTGCTTG+TGG 0.614746 4_4:-66492699 Msa0696530:CDS
AAATTTGAACAATCTCCTGA+TGG 0.624669 4_4:+66492057 None:intergenic
GTTTAACAAGTTCACTAACA+AGG 0.624782 4_4:-66492399 Msa0696530:CDS
ATAACCCTCTAGCCCAGGAA+GGG 0.629515 4_4:+66490119 None:intergenic
GGTTTGGGTATCAGTTGTCG+TGG 0.630699 4_4:-66489946 Msa0696530:three_prime_UTR
AGCCCCTTAAATGGTACATG+TGG 0.636992 4_4:-66491717 Msa0696530:CDS
TAATCATTCTGAGAAACTTG+AGG 0.638838 4_4:-66492847 Msa0696530:CDS
TTGTACAATAATACTCCGGT+TGG 0.640064 4_4:+66490304 None:intergenic
AAAGTCGAAGAATTGTGGCA+GGG 0.644806 4_4:+66491003 None:intergenic
TGATTGCAAATGCAGTGCCA+TGG 0.662207 4_4:+66493136 None:intergenic
GACGATTTGAGGTTTCAAAG+AGG 0.663177 4_4:+66493106 None:intergenic
TCATTCTGAGAAACTTGAGG+TGG 0.664975 4_4:-66492844 Msa0696530:CDS
GGATGATTTAGGAAACGCAG+AGG 0.673607 4_4:-66490979 Msa0696530:intron
TTTCCATAAAGCATTCACAA+AGG 0.678455 4_4:-66490199 Msa0696530:CDS
TTCAGGCTATTAAGACAACA+AGG 0.681647 4_4:-66492636 Msa0696530:CDS
AAGAAGAAGAGCAACAGACG+AGG 0.695455 4_4:-66490283 Msa0696530:CDS
TAATAGCCTGAAGTGAAGTG+CGG 0.695756 4_4:+66492647 None:intergenic
TATGTCGCTAGTGCTTGTGG+TGG 0.710715 4_4:-66492696 Msa0696530:CDS
CAGATAAAGTAGGATCCGTG+AGG 0.735628 4_4:+66491683 None:intergenic
ATATAGGACGAAAATCAACA+AGG 0.742989 4_4:-66490363 Msa0696530:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAAAATTAAAAATTA+AGG + chr4_4:66491215-66491234 None:intergenic 0.0%
!!! TTTTAATTTTTAATTTATTT+TGG - chr4_4:66491219-66491238 Msa0696530:intron 0.0%
!! AAAATTAAAAATTAAGGAAT+AGG + chr4_4:66491209-66491228 None:intergenic 10.0%
!! AATTAATGATTAATAATTGT+TGG - chr4_4:66491523-66491542 Msa0696530:intron 10.0%
!! ATTAATGATTAATAATTGTT+GGG - chr4_4:66491524-66491543 Msa0696530:intron 10.0%
!!! ATAAAACTTCTTTTTATATA+TGG + chr4_4:66492218-66492237 None:intergenic 10.0%
!! TATTATTATTCTATAATATG+TGG - chr4_4:66492590-66492609 Msa0696530:intron 10.0%
!! GATAGTAGTAAATTAAAATT+TGG + chr4_4:66490060-66490079 None:intergenic 15.0%
!!! ATTTTAATTTACTACTATCT+TGG - chr4_4:66490061-66490080 Msa0696530:three_prime_UTR 15.0%
!! GAATAATTTATTGAGATTAA+AGG - chr4_4:66491008-66491027 Msa0696530:CDS 15.0%
!! ATTATGTAGATTAAAGATAT+TGG + chr4_4:66491380-66491399 None:intergenic 15.0%
!!! ATGACATTTTTGATATTTAT+GGG - chr4_4:66491402-66491421 Msa0696530:CDS 15.0%
!!! ATAGTTTAGAGTTTAATTTA+AGG - chr4_4:66492073-66492092 Msa0696530:CDS 15.0%
!! AAATGATTGTTATATACTTA+GGG + chr4_4:66492242-66492261 None:intergenic 15.0%
!! AAAATGATTGTTATATACTT+AGG + chr4_4:66492243-66492262 None:intergenic 15.0%
!!! TCATCTACTATATTTTTTAA+AGG + chr4_4:66492333-66492352 None:intergenic 15.0%
!!! TCTTTTTAATTTATATTGGG+AGG - chr4_4:66490558-66490577 Msa0696530:intron 20.0%
!!! ATGTATGCTAATTAATTTGA+TGG - chr4_4:66490986-66491005 Msa0696530:CDS 20.0%
!!! TAAAACGGGTTTTAAATAAA+AGG + chr4_4:66491113-66491132 None:intergenic 20.0%
!!! TTAATTTATTTTGGTGTAGA+TGG - chr4_4:66491228-66491247 Msa0696530:intron 20.0%
!!! GATGACATTTTTGATATTTA+TGG - chr4_4:66491401-66491420 Msa0696530:CDS 20.0%
!!! AAACATGTTTTCATGTAATT+TGG + chr4_4:66491586-66491605 None:intergenic 20.0%
!! TAAGCATAAGTAAGTAATAT+AGG - chr4_4:66491754-66491773 Msa0696530:CDS 20.0%
!! TTATATATGGACTTATATGA+TGG + chr4_4:66492205-66492224 None:intergenic 20.0%
!!! ACTATTTTCATCATATGTTT+GGG - chr4_4:66492448-66492467 Msa0696530:intron 20.0%
!! ACAAGTCAATAAATCATAAT+TGG + chr4_4:66492528-66492547 None:intergenic 20.0%
!! AAAAACTTTATCATAAAGCA+AGG + chr4_4:66492928-66492947 None:intergenic 20.0%
!! TTCTTCAAAAAAAACTCAAT+AGG - chr4_4:66493019-66493038 Msa0696530:CDS 20.0%
!! TCTTCAAAAAAAACTCAATA+GGG - chr4_4:66493020-66493039 Msa0696530:CDS 20.0%
!!! AATAATTTATTTTGCTAAGC+AGG - chr4_4:66493047-66493066 Msa0696530:CDS 20.0%
! CAAAGTGGTAATCAATATTT+AGG + chr4_4:66490266-66490285 None:intergenic 25.0%
! AAAAAAATGAAGTGCATGTT+TGG + chr4_4:66490498-66490517 None:intergenic 25.0%
!! TTGATATTTTCACCTAGTTT+TGG + chr4_4:66490655-66490674 None:intergenic 25.0%
! AAACTAGGTGAAAATATCAA+TGG - chr4_4:66490655-66490674 Msa0696530:intron 25.0%
!! ATTGGCAAATTTTTGTGAAA+AGG + chr4_4:66490832-66490851 None:intergenic 25.0%
! ATTTATATAGCACAAATGCA+TGG - chr4_4:66490878-66490897 Msa0696530:intron 25.0%
!! AAGAGTTGTGATTGAATTTT+AGG - chr4_4:66491156-66491175 Msa0696530:CDS 25.0%
!! AGAGTTGTGATTGAATTTTA+GGG - chr4_4:66491157-66491176 Msa0696530:CDS 25.0%
! CTTGTTACATTACATTTCTT+AGG - chr4_4:66491480-66491499 Msa0696530:intron 25.0%
! ATTACATGAAAACATGTTTC+AGG - chr4_4:66491587-66491606 Msa0696530:CDS 25.0%
! TTACATGAAAACATGTTTCA+GGG - chr4_4:66491588-66491607 Msa0696530:CDS 25.0%
! TCCAAAATATATAAGAAGCA+TGG - chr4_4:66491640-66491659 Msa0696530:CDS 25.0%
!! CCAAAATATATAAGAAGCAT+GGG - chr4_4:66491641-66491660 Msa0696530:CDS 25.0%
! TTTAAGTATTCTTCAGCATT+TGG + chr4_4:66491871-66491890 None:intergenic 25.0%
! CTGAAGAATACTTAAAGAAT+GGG - chr4_4:66491875-66491894 Msa0696530:intron 25.0%
!! GTTCACATTTTCTTTCTTTT+GGG - chr4_4:66491928-66491947 Msa0696530:intron 25.0%
!!! ATTTTCTTTCTTTTGGGTAA+AGG - chr4_4:66491934-66491953 Msa0696530:intron 25.0%
! TAAGGAAAATGTTCATACAA+TGG - chr4_4:66491963-66491982 Msa0696530:intron 25.0%
!!! TCTTGTGATTGATTTTCATT+GGG + chr4_4:66492367-66492386 None:intergenic 25.0%
!!! TTCTTGTGATTGATTTTCAT+TGG + chr4_4:66492368-66492387 None:intergenic 25.0%
!! GACTATTTTCATCATATGTT+TGG - chr4_4:66492447-66492466 Msa0696530:intron 25.0%
! GTTTGTGACAACATAAATAT+AGG - chr4_4:66492635-66492654 Msa0696530:CDS 25.0%
! TCATCATAACTATACATCAA+TGG + chr4_4:66492867-66492886 None:intergenic 25.0%
!!! GGTATTGGGATTAATAATTT+TGG + chr4_4:66492961-66492980 None:intergenic 25.0%
! ATTGAGTTATTCCTTCATAT+TGG + chr4_4:66492990-66493009 None:intergenic 25.0%
! ATATGAAGGAATAACTCAAT+AGG - chr4_4:66492990-66493009 Msa0696530:CDS 25.0%
! TATGAAGGAATAACTCAATA+GGG - chr4_4:66492991-66493010 Msa0696530:CDS 25.0%
! CTTCAAAAAAAACTCAATAG+GGG - chr4_4:66493021-66493040 Msa0696530:CDS 25.0%
!! TGTATGTTTAGTAGTCTTTT+TGG - chr4_4:66493154-66493173 Msa0696530:exon 25.0%
AATATACGTAAGTCAAGTAG+TGG + chr4_4:66489848-66489867 None:intergenic 30.0%
GCATTAATGTTGAAGAAGAA+AGG - chr4_4:66490004-66490023 Msa0696530:three_prime_UTR 30.0%
! TAATCATTCTGAGAAACTTG+AGG - chr4_4:66490167-66490186 Msa0696530:CDS 30.0%
GTTCAATTTCTTCTTCAAAG+TGG + chr4_4:66490281-66490300 None:intergenic 30.0%
AAATTGCTCTTAAGTTATGC+AGG + chr4_4:66490538-66490557 None:intergenic 30.0%
GTTTAACAAGTTCACTAACA+AGG - chr4_4:66490615-66490634 Msa0696530:intron 30.0%
GAAAAGTTTGATCCAAAACT+AGG - chr4_4:66490640-66490659 Msa0696530:intron 30.0%
GAAGATCCTTAAGGAAAAAA+TGG - chr4_4:66490756-66490775 Msa0696530:intron 30.0%
!!! ATTGTGCCATTTTTTCCTTA+AGG + chr4_4:66490765-66490784 None:intergenic 30.0%
TTTGTGAAAAGGATACTCTA+AGG + chr4_4:66490821-66490840 None:intergenic 30.0%
!! AAATTTCTAGCTGTGAACAT+TGG + chr4_4:66490850-66490869 None:intergenic 30.0%
TTCACAGCTAGAAATTTCTA+TGG - chr4_4:66490853-66490872 Msa0696530:intron 30.0%
TATGAAAGAAGTTGCGAAAA+TGG - chr4_4:66490909-66490928 Msa0696530:intron 30.0%
AAATTTGAACAATCTCCTGA+TGG + chr4_4:66490960-66490979 None:intergenic 30.0%
GAAGAGTTGTGATTAATTGT+CGG + chr4_4:66491065-66491084 None:intergenic 30.0%
ACGTCATGTTAAAGTAAAAC+GGG + chr4_4:66491127-66491146 None:intergenic 30.0%
CACGTCATGTTAAAGTAAAA+CGG + chr4_4:66491128-66491147 None:intergenic 30.0%
!!! ATTTATTTTGGTGTAGATGG+TGG - chr4_4:66491231-66491250 Msa0696530:intron 30.0%
ATTCTTTCCTCAGATAAAGT+AGG + chr4_4:66491344-66491363 None:intergenic 30.0%
! GTTCATTTGTTAGATCATAG+AGG + chr4_4:66491615-66491634 None:intergenic 30.0%
! CCCATGCTTCTTATATATTT+TGG + chr4_4:66491644-66491663 None:intergenic 30.0%
! AAATATATAAGAAGCATGGG+TGG - chr4_4:66491644-66491663 Msa0696530:CDS 30.0%
! AATATATAAGAAGCATGGGT+GGG - chr4_4:66491645-66491664 Msa0696530:CDS 30.0%
GCTGAAGAATACTTAAAGAA+TGG - chr4_4:66491874-66491893 Msa0696530:intron 30.0%
! GGTTCACATTTTCTTTCTTT+TGG - chr4_4:66491927-66491946 Msa0696530:intron 30.0%
TTTGGGTAAAGGATTAACTA+AGG - chr4_4:66491945-66491964 Msa0696530:intron 30.0%
ATATAGGACGAAAATCAACA+AGG - chr4_4:66492651-66492670 Msa0696530:CDS 30.0%
GAAAATCAACAAGGAAATGA+TGG - chr4_4:66492660-66492679 Msa0696530:CDS 30.0%
CTTCTTGTACAATAATACTC+CGG + chr4_4:66492717-66492736 None:intergenic 30.0%
TGAATAAAATATCCGATGCA+TGG - chr4_4:66492757-66492776 Msa0696530:CDS 30.0%
TGTGAATGCTTTATGGAAAT+GGG + chr4_4:66492814-66492833 None:intergenic 30.0%
TTGTGAATGCTTTATGGAAA+TGG + chr4_4:66492815-66492834 None:intergenic 30.0%
! TTTCCATAAAGCATTCACAA+AGG - chr4_4:66492815-66492834 Msa0696530:CDS 30.0%
CTTTATCATAAAGCAAGGAA+TGG + chr4_4:66492923-66492942 None:intergenic 30.0%
! ATTCCTTCATATTGGCTAAT+GGG + chr4_4:66492982-66493001 None:intergenic 30.0%
! TATTCCTTCATATTGGCTAA+TGG + chr4_4:66492983-66493002 None:intergenic 30.0%
ATGAAGGAATAACTCAATAG+GGG - chr4_4:66492992-66493011 Msa0696530:CDS 30.0%
!!! TTTATTTTGCTAAGCAGGTT+TGG - chr4_4:66493052-66493071 Msa0696530:CDS 30.0%
!! TTATTTTGCTAAGCAGGTTT+GGG - chr4_4:66493053-66493072 Msa0696530:CDS 30.0%
TCAAATCCGCTAATATGTTT+GGG - chr4_4:66493091-66493110 Msa0696530:CDS 30.0%
!!! TCTGTTTTGTTGTTCTTCAT+TGG - chr4_4:66493125-66493144 Msa0696530:CDS 30.0%
GTATATTCATTCCTTTGCCA+TGG - chr4_4:66489861-66489880 Msa0696530:three_prime_UTR 35.0%
! TTTGAGGTTTCAAAGAGGAA+AGG + chr4_4:66489906-66489925 None:intergenic 35.0%
GAGAAAGTTGTGCAATTGTT+GGG + chr4_4:66489933-66489952 None:intergenic 35.0%
TGAGAAAGTTGTGCAATTGT+TGG + chr4_4:66489934-66489953 None:intergenic 35.0%
AAGCATGAACTTAGAAACGT+TGG - chr4_4:66490195-66490214 Msa0696530:CDS 35.0%
! GTTGGTGAAAATTCACTCAA+AGG - chr4_4:66490213-66490232 Msa0696530:CDS 35.0%
TAGCGACATATTGTCTTCTT+AGG + chr4_4:66490308-66490327 None:intergenic 35.0%
TTCAGGCTATTAAGACAACA+AGG - chr4_4:66490378-66490397 Msa0696530:intron 35.0%
TATGCAGGAAAAGGATCAAA+AGG + chr4_4:66490523-66490542 None:intergenic 35.0%
CTCTTAAGTTATGCAGGAAA+AGG + chr4_4:66490532-66490551 None:intergenic 35.0%
ATTCGAATTTACTATCGCAG+AGG - chr4_4:66490591-66490610 Msa0696530:intron 35.0%
ATGAAGCTGGAAAATTTAGC+AGG - chr4_4:66490734-66490753 Msa0696530:intron 35.0%
ATTTAGCAGGAAGATCCTTA+AGG - chr4_4:66490747-66490766 Msa0696530:intron 35.0%
TTCACGAAGCTAAGTTTGTA+AGG - chr4_4:66490791-66490810 Msa0696530:intron 35.0%
TATAGCACAAATGCATGGTT+TGG - chr4_4:66490883-66490902 Msa0696530:intron 35.0%
! CGTTTTACTTTAACATGACG+TGG - chr4_4:66491126-66491145 Msa0696530:CDS 35.0%
! AATTAAGGAATAGGCGTTGT+CGG + chr4_4:66491200-66491219 None:intergenic 35.0%
TGACCACATGTACCATTTAA+GGG + chr4_4:66491303-66491322 None:intergenic 35.0%
ATGACCACATGTACCATTTA+AGG + chr4_4:66491304-66491323 None:intergenic 35.0%
TAAATGGTACATGTGGTCAT+TGG - chr4_4:66491304-66491323 Msa0696530:intron 35.0%
AAATGGTACATGTGGTCATT+GGG - chr4_4:66491305-66491324 Msa0696530:intron 35.0%
!! TGTTCACTGAAGTTGTTTCT+AGG - chr4_4:66491444-66491463 Msa0696530:CDS 35.0%
TAATAATTGTTGGGTGCAGA+TGG - chr4_4:66491533-66491552 Msa0696530:intron 35.0%
AATAATTGTTGGGTGCAGAT+GGG - chr4_4:66491534-66491553 Msa0696530:intron 35.0%
ATGGGATATTGACACAGATA+TGG - chr4_4:66491552-66491571 Msa0696530:intron 35.0%
TCTTTCGAAGAGAGTCTTTA+GGG + chr4_4:66491672-66491691 None:intergenic 35.0%
TAAAGACTCTCTTCGAAAGA+CGG - chr4_4:66491672-66491691 Msa0696530:CDS 35.0%
! ATCTTTTACCATTTGTGCAG+TGG - chr4_4:66491788-66491807 Msa0696530:intron 35.0%
! TCTTTTACCATTTGTGCAGT+GGG - chr4_4:66491789-66491808 Msa0696530:intron 35.0%
! CCATTTTGCTTCAACTAGAA+AGG + chr4_4:66491830-66491849 None:intergenic 35.0%
!! CCTTTCTAGTTGAAGCAAAA+TGG - chr4_4:66491827-66491846 Msa0696530:intron 35.0%
CTAGTTGAAGCAAAATGGTT+TGG - chr4_4:66491832-66491851 Msa0696530:intron 35.0%
!! AAGCAAAATGGTTTGGTTCA+GGG - chr4_4:66491839-66491858 Msa0696530:intron 35.0%
! ATACGTGAAGTGTCTAATGA+TGG - chr4_4:66492686-66492705 Msa0696530:CDS 35.0%
TTGTACAATAATACTCCGGT+TGG + chr4_4:66492713-66492732 None:intergenic 35.0%
AGACCTTTGTGAATGCTTTA+TGG + chr4_4:66492821-66492840 None:intergenic 35.0%
! CATATTGGCTAATGGGTATT+GGG + chr4_4:66492975-66492994 None:intergenic 35.0%
! TCATATTGGCTAATGGGTAT+TGG + chr4_4:66492976-66492995 None:intergenic 35.0%
ATACCCATTAGCCAATATGA+AGG - chr4_4:66492976-66492995 Msa0696530:intron 35.0%
GTCAAATCCGCTAATATGTT+TGG - chr4_4:66493090-66493109 Msa0696530:CDS 35.0%
! GACGATTTGAGGTTTCAAAG+AGG + chr4_4:66489911-66489930 None:intergenic 40.0%
CTCTTCACATTGCTAACAAG+TGG - chr4_4:66489980-66489999 Msa0696530:three_prime_UTR 40.0%
! TCATTCTGAGAAACTTGAGG+TGG - chr4_4:66490170-66490189 Msa0696530:CDS 40.0%
! ATCAATATTTAGGCGCTGCA+TGG + chr4_4:66490256-66490275 None:intergenic 40.0%
TAATAGCCTGAAGTGAAGTG+CGG + chr4_4:66490370-66490389 None:intergenic 40.0%
ACGCACTTAAAACTAACCTG+CGG + chr4_4:66490415-66490434 None:intergenic 40.0%
! GCATGTTTGGAATTACAGTG+AGG + chr4_4:66490485-66490504 None:intergenic 40.0%
GAAGACATACTTGATGAAGC+TGG - chr4_4:66490721-66490740 Msa0696530:intron 40.0%
!! ATGGATTTCAGTTTGCTGCA+AGG - chr4_4:66490928-66490947 Msa0696530:intron 40.0%
GACCACATGTACCATTTAAG+GGG + chr4_4:66491302-66491321 None:intergenic 40.0%
GTCTTTCGAAGAGAGTCTTT+AGG + chr4_4:66491673-66491692 None:intergenic 40.0%
!! GAAGCAAAATGGTTTGGTTC+AGG - chr4_4:66491838-66491857 Msa0696530:intron 40.0%
AAGAATGGGATAGTAAGCTC+TGG - chr4_4:66491889-66491908 Msa0696530:intron 40.0%
AAAGTCGAAGAATTGTGGCA+GGG + chr4_4:66492014-66492033 None:intergenic 40.0%
TGCCACAATTCTTCGACTTT+GGG - chr4_4:66492014-66492033 Msa0696530:intron 40.0%
ATCCCAAAGTCGAAGAATTG+TGG + chr4_4:66492019-66492038 None:intergenic 40.0%
!! CTTCGACTTTGGGATGATTT+AGG - chr4_4:66492024-66492043 Msa0696530:intron 40.0%
CCGATACCCAAACATATTAG+CGG + chr4_4:66493100-66493119 None:intergenic 40.0%
CCGCTAATATGTTTGGGTAT+CGG - chr4_4:66493097-66493116 Msa0696530:CDS 40.0%
TGATTGCAAATGCAGTGCCA+TGG + chr4_4:66489881-66489900 None:intergenic 45.0%
GCAATTGTTGGGACGATTTG+AGG + chr4_4:66489922-66489941 None:intergenic 45.0%
CAATATGTCGCTAGTGCTTG+TGG - chr4_4:66490315-66490334 Msa0696530:CDS 45.0%
CGCACTTAAAACTAACCTGC+GGG + chr4_4:66490414-66490433 None:intergenic 45.0%
!! TGTAGATGGTGGAGAGAACT+TGG - chr4_4:66491242-66491261 Msa0696530:intron 45.0%
CTAGAAACCAGCCCCTTAAA+TGG - chr4_4:66491288-66491307 Msa0696530:intron 45.0%
ACATGTACCATTTAAGGGGC+TGG + chr4_4:66491298-66491317 None:intergenic 45.0%
AGCCCCTTAAATGGTACATG+TGG - chr4_4:66491297-66491316 Msa0696530:intron 45.0%
CAGATAAAGTAGGATCCGTG+AGG + chr4_4:66491334-66491353 None:intergenic 45.0%
CACGGATCCTACTTTATCTG+AGG - chr4_4:66491334-66491353 Msa0696530:intron 45.0%
AAACTCTCCCACTGCACAAA+TGG + chr4_4:66491799-66491818 None:intergenic 45.0%
!! AGGGGCGGAAATAATGGATA+GGG + chr4_4:66491995-66492014 None:intergenic 45.0%
AAGTCGAAGAATTGTGGCAG+GGG + chr4_4:66492013-66492032 None:intergenic 45.0%
CAAAGTCGAAGAATTGTGGC+AGG + chr4_4:66492015-66492034 None:intergenic 45.0%
CTGCCACAATTCTTCGACTT+TGG - chr4_4:66492013-66492032 Msa0696530:intron 45.0%
GGATGATTTAGGAAACGCAG+AGG - chr4_4:66492035-66492054 Msa0696530:intron 45.0%
!! GTGTCTAATGATGGACCAAC+CGG - chr4_4:66492695-66492714 Msa0696530:CDS 45.0%
AAGAAGAAGAGCAACAGACG+AGG - chr4_4:66492731-66492750 Msa0696530:CDS 45.0%
! GTTGAGGCTTTTCCATGCAT+CGG + chr4_4:66492772-66492791 None:intergenic 45.0%
CAAAGAGACACTCTTGGTTG+AGG + chr4_4:66492788-66492807 None:intergenic 45.0%
GGGTATCAAAGAGACACTCT+TGG + chr4_4:66492794-66492813 None:intergenic 45.0%
AGGTCTCACTCAATCTTGCA+AGG - chr4_4:66492835-66492854 Msa0696530:CDS 45.0%
CTCACTCAATCTTGCAAGGA+TGG - chr4_4:66492839-66492858 Msa0696530:CDS 45.0%
ATGATAAGCACTCCCTTCCT+GGG - chr4_4:66492883-66492902 Msa0696530:intron 45.0%
!!! ATTTTCTTTTTAATTTATAT+TGG - chr4_4:66490554-66490573 Msa0696530:intron 5.0%
!!! TTTTCTTTTTAATTTATATT+GGG - chr4_4:66490555-66490574 Msa0696530:intron 5.0%
!!! TTAATCATTAATTTTTTTTT+TGG + chr4_4:66491516-66491535 None:intergenic 5.0%
CAAATGCAGTGCCATGGCAA+AGG + chr4_4:66489875-66489894 None:intergenic 50.0%
TATGTCGCTAGTGCTTGTGG+TGG - chr4_4:66490318-66490337 Msa0696530:CDS 50.0%
ATGAGACCGCACTTCACTTC+AGG - chr4_4:66490361-66490380 Msa0696530:intron 50.0%
TGAAGCCTCGCATCTAAACC+TGG - chr4_4:66490693-66490712 Msa0696530:intron 50.0%
!! AGGGGCTGGTTTCTAGCATA+TGG + chr4_4:66491284-66491303 None:intergenic 50.0%
!! CTCTGGAGTGCATATTGTGC+TGG - chr4_4:66491906-66491925 Msa0696530:intron 50.0%
!! CAGGGGCGGAAATAATGGAT+AGG + chr4_4:66491996-66492015 None:intergenic 50.0%
GATGATAAGCACTCCCTTCC+TGG - chr4_4:66492882-66492901 Msa0696530:intron 50.0%
ATAACCCTCTAGCCCAGGAA+GGG + chr4_4:66492898-66492917 None:intergenic 50.0%
GGTTTGGGTATCAGTTGTCG+TGG - chr4_4:66493068-66493087 Msa0696530:CDS 50.0%
ACAGCAATCCCTTCAGCACA+AGG + chr4_4:66493184-66493203 None:intergenic 50.0%
TTGTGCTGAAGGGATTGCTG+TGG - chr4_4:66493183-66493202 Msa0696530:five_prime_UTR 50.0%
! CAAGGTCACTTTGTTCCCGC+AGG - chr4_4:66490396-66490415 Msa0696530:intron 55.0%
CCTCGCATCTAAACCTGGCA+GGG - chr4_4:66490698-66490717 Msa0696530:intron 55.0%
CTCGCATCTAAACCTGGCAG+GGG - chr4_4:66490699-66490718 Msa0696530:intron 55.0%
CAAGTATGTCTTCCCCTGCC+AGG + chr4_4:66490714-66490733 None:intergenic 55.0%
GCTGCAAGGCTTACACCATC+AGG - chr4_4:66490942-66490961 Msa0696530:intron 55.0%
GTGGTCATTGGGATGCCTCA+CGG - chr4_4:66491316-66491335 Msa0696530:intron 55.0%
TGTGGCAGGGGCGGAAATAA+TGG + chr4_4:66492001-66492020 None:intergenic 55.0%
TCGAAGAATTGTGGCAGGGG+CGG + chr4_4:66492010-66492029 None:intergenic 55.0%
CATAACCCTCTAGCCCAGGA+AGG + chr4_4:66492899-66492918 None:intergenic 55.0%
TGGACATAACCCTCTAGCCC+AGG + chr4_4:66492903-66492922 None:intergenic 55.0%
TTTGGCACGCCTTGTGCTGA+AGG - chr4_4:66493172-66493191 Msa0696530:five_prime_UTR 55.0%
TTGGCACGCCTTGTGCTGAA+GGG - chr4_4:66493173-66493192 Msa0696530:five_prime_UTR 55.0%
GCCTCGCATCTAAACCTGGC+AGG - chr4_4:66490697-66490716 Msa0696530:intron 60.0%
CCCTGCCAGGTTTAGATGCG+AGG + chr4_4:66490701-66490720 None:intergenic 60.0%
! CACTCCCTTCCTGGGCTAGA+GGG - chr4_4:66492891-66492910 Msa0696530:intron 60.0%
! GCACTCCCTTCCTGGGCTAG+AGG - chr4_4:66492890-66492909 Msa0696530:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 66489807 66493229 66489807 ID=Msa0696530;Name=Msa0696530
chr4_4 mRNA 66489807 66493229 66489807 ID=Msa0696530-mRNA-1;Parent=Msa0696530;Name=Msa0696530-mRNA-1;_AED=0.06;_eAED=0.06;_QI=73|1|1|1|1|1|7|279|572
chr4_4 exon 66489807 66490379 66489807 ID=Msa0696530-mRNA-1:exon:19372;Parent=Msa0696530-mRNA-1
chr4_4 exon 66490980 66491228 66490980 ID=Msa0696530-mRNA-1:exon:19371;Parent=Msa0696530-mRNA-1
chr4_4 exon 66491343 66491484 66491343 ID=Msa0696530-mRNA-1:exon:19370;Parent=Msa0696530-mRNA-1
chr4_4 exon 66491571 66491789 66491571 ID=Msa0696530-mRNA-1:exon:19369;Parent=Msa0696530-mRNA-1
chr4_4 exon 66492053 66492425 66492053 ID=Msa0696530-mRNA-1:exon:19368;Parent=Msa0696530-mRNA-1
chr4_4 exon 66492619 66492889 66492619 ID=Msa0696530-mRNA-1:exon:19367;Parent=Msa0696530-mRNA-1
chr4_4 exon 66492986 66493229 66492986 ID=Msa0696530-mRNA-1:exon:19366;Parent=Msa0696530-mRNA-1
chr4_4 five_prime_UTR 66493157 66493229 66493157 ID=Msa0696530-mRNA-1:five_prime_utr;Parent=Msa0696530-mRNA-1
chr4_4 CDS 66492986 66493156 66492986 ID=Msa0696530-mRNA-1:cds;Parent=Msa0696530-mRNA-1
chr4_4 CDS 66492619 66492889 66492619 ID=Msa0696530-mRNA-1:cds;Parent=Msa0696530-mRNA-1
chr4_4 CDS 66492053 66492425 66492053 ID=Msa0696530-mRNA-1:cds;Parent=Msa0696530-mRNA-1
chr4_4 CDS 66491571 66491789 66491571 ID=Msa0696530-mRNA-1:cds;Parent=Msa0696530-mRNA-1
chr4_4 CDS 66491343 66491484 66491343 ID=Msa0696530-mRNA-1:cds;Parent=Msa0696530-mRNA-1
chr4_4 CDS 66490980 66491228 66490980 ID=Msa0696530-mRNA-1:cds;Parent=Msa0696530-mRNA-1
chr4_4 CDS 66490086 66490379 66490086 ID=Msa0696530-mRNA-1:cds;Parent=Msa0696530-mRNA-1
chr4_4 three_prime_UTR 66489807 66490085 66489807 ID=Msa0696530-mRNA-1:three_prime_utr;Parent=Msa0696530-mRNA-1
Gene Sequence

>Msa0696530

ATGGCACTGCATTTGCAATCATGCCTTTCCTCTTTGAAACCTCAAATCGTCCCAACAATTGCACAACTTTCTCAAAACTTTAATAATCTCAAGCTTGATTCTCTTCACATTGCTAACAAGTGGAGCATTAATGTTGAAGAAGAAAGGCGTAGTGCACTCATAAAACAACATAGAGATCTCAGCAGCAATAATAATCATTCTGAGAAACTTGAGGTGGTTAAGCATGAACTTAGAAACGTTGGTGAAAATTCACTCAAAGGTTTGTATATGATTGATGCCATGCAGCGCCTAAATATTGATTACCACTTTGAAGAAGAAATTGAACAATTCCTAAGAAGACAATATGTCGCTAGTGCTTGTGGTGGAAGTAATCATCATAATCTTCATGAGACCGCACTTCACTTCAGGCTATTAAGACAACAAGGTCACTTTGTTCCCGCAGAGGTGTTTAACAAGTTCACTAACAAGGATGAAAAGTTTGATCCAAAACTAGGTGAAAATATCAATGGAATGATAGATTTATTTGAAGCCTCGCATCTAAACCTGGCAGGGGAAGACATACTTGATGAAGCTGGAAAATTTAGCAGGAAGATCCTTAAGGAAAAAATGGCACAATTTGATTTTCACGAAGCTAAGTTTGTAAGGAGAACCTTAGAGTATCCTTTTCACAAAAATTTGCCAATGTTCACAGCTAGAAATTTCTATGGTCATTTATATAGCACAAATGCATGGTTTGGTTCTATGAAAGAAGTTGCGAAAATGGATTTCAGTTTGCTGCAAGGCTTACACCATCAGGAGATTGTTCAAATTTCAAAATGGTGGAGAGAACTTGGTTTAGCTAATGAATTACCATATGCTAGAAACCAGCCCCTTAAATGGTACATGTGGTCATTGGGATGCCTCACGGATCCTACTTTATCTGAGGAAAGAATTGAGCTCACAAAACCAATATCTTTAATCTACATAATTGATGACATTTTTGATATTTATGGGACTCTAGATGAGTTAACTCTGTTCACTGAAGTTGTTTCTAGATGGGATATTGACACAGATATGGAGCAACTACCAAATTACATGAAAACATGTTTCAGGGTCCTCTATGATCTAACAAATGAACTAAGCTCCAAAATATATAAGAAGCATGGGTGGGACCCTAAAGACTCTCTTCGAAAGACGTGGGAGAGTTTGTGCAAAGCCTTTCTAGTTGAAGCAAAATGGTTTGGTTCAGGGAAGTTGCCAAATGCTGAAGAATACTTAAAGAATGGGATAGTAAGCTCTGGAGTGCATATTGTGCTGGTTCACATTTTCTTTCTTTTGGGTAAAGGATTAACTAAGGAAAATGTTCATACAATGGACACAACCCTATCCATTATTTCCGCCCCTGCCACAATTCTTCGACTTTGGGATGATTTAGGAAACGCAGAGGACGAAAATCAACAAGGAAATGATGGATCATACGTGAAGTGTCTAATGATGGACCAACCGGAGTATTATTGTACAAGAAGAAGAGCAACAGACGAGGTTATGAATAAAATATCCGATGCATGGAAAAGCCTCAACCAAGAGTGTCTCTTTGATACCCATTTCCATAAAGCATTCACAAAGGTCTCACTCAATCTTGCAAGGATGGTACCATTGATGTATAGTTATGATGATAAGCACTCCCTTCCTGGGCTAGAGGGTTATGTCCATTCCTTGCTTTATGATAAAGTTTTTTGA
Protein sequence

>Msa0696530

MALHLQSCLSSLKPQIVPTIAQLSQNFNNLKLDSLHIANKWSINVEEERRSALIKQHRDLSSNNNHSEKLEVVKHELRNVGENSLKGLYMIDAMQRLNIDYHFEEEIEQFLRRQYVASACGGSNHHNLHETALHFRLLRQQGHFVPAEVFNKFTNKDEKFDPKLGENINGMIDLFEASHLNLAGEDILDEAGKFSRKILKEKMAQFDFHEAKFVRRTLEYPFHKNLPMFTARNFYGHLYSTNAWFGSMKEVAKMDFSLLQGLHHQEIVQISKWWRELGLANELPYARNQPLKWYMWSLGCLTDPTLSEERIELTKPISLIYIIDDIFDIYGTLDELTLFTEVVSRWDIDTDMEQLPNYMKTCFRVLYDLTNELSSKIYKKHGWDPKDSLRKTWESLCKAFLVEAKWFGSGKLPNAEEYLKNGIVSSGVHIVLVHIFFLLGKGLTKENVHTMDTTLSIISAPATILRLWDDLGNAEDENQQGNDGSYVKCLMMDQPEYYCTRRRATDEVMNKISDAWKSLNQECLFDTHFHKAFTKVSLNLARMVPLMYSYDDKHSLPGLEGYVHSLLYDKVF*