AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0640220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0640220 MtrunA17_Chr4g0023911 79.690 581 40 5 1 504 1 580 0.0 924
Msa0640220 MtrunA17_Chr2g0310331 41.101 545 243 10 35 503 42 584 1.71e-125 378
Msa0640220 MtrunA17_Chr8g0357411 37.004 554 265 12 32 503 36 587 5.46e-108 333
Msa0640220 MtrunA17_Chr2g0310401 37.850 428 193 6 147 503 5 430 5.73e-90 281
Msa0640220 MtrunA17_Chr4g0042111 30.270 555 302 10 31 502 7 559 6.05e-78 254
Msa0640220 MtrunA17_Chr2g0323151 33.188 458 233 6 96 482 4 459 5.76e-75 244
Msa0640220 MtrunA17_Chr6g0466051 29.197 548 305 8 38 503 5 551 5.24e-73 241
Msa0640220 MtrunA17_Chr6g0465911 29.197 548 305 8 38 503 5 551 5.76e-73 241
Msa0640220 MtrunA17_Chr6g0465441 28.285 548 310 8 38 503 5 551 1.27e-72 240
Msa0640220 MtrunA17_Chr6g0451711 28.339 554 308 8 35 503 6 555 2.38e-72 239
Msa0640220 MtrunA17_Chr2g0323131 31.974 466 241 8 90 482 82 544 3.11e-72 239
Msa0640220 MtrunA17_Chr2g0319051 31.631 509 259 12 81 504 9 513 9.48e-72 237
Msa0640220 MtrunA17_Chr4g0024691 29.273 550 307 9 35 503 11 559 1.80e-71 237
Msa0640220 MtrunA17_Chr3g0082691 30.000 540 295 8 46 503 13 551 4.42e-71 236
Msa0640220 MtrunA17_Chr5g0425371 27.860 542 309 7 43 503 11 551 5.38e-69 230
Msa0640220 MtrunA17_Chr6g0465851 29.183 514 283 6 70 503 4 516 1.02e-68 229
Msa0640220 MtrunA17_Chr7g0237001 28.650 548 297 9 43 503 11 551 1.99e-68 229
Msa0640220 MtrunA17_Chr7g0237041 28.650 548 297 9 43 503 11 551 1.99e-68 229
Msa0640220 MtrunA17_Chr7g0237081 28.650 548 297 9 43 503 11 551 1.99e-68 229
Msa0640220 MtrunA17_Chr7g0237051 28.650 548 297 9 43 503 11 551 1.99e-68 229
Msa0640220 MtrunA17_Chr7g0236991 28.467 548 298 9 43 503 11 551 7.68e-68 227
Msa0640220 MtrunA17_Chr8g0336181 32.000 550 274 16 46 503 13 554 1.78e-67 226
Msa0640220 MtrunA17_Chr3g0105071 29.730 555 307 11 31 503 2 555 4.90e-67 225
Msa0640220 MtrunA17_Chr2g0319041 29.730 555 300 13 37 504 5 556 1.18e-65 222
Msa0640220 MtrunA17_Chr5g0431651 29.348 552 296 11 40 503 5 550 4.63e-65 220
Msa0640220 MtrunA17_Chr6g0465981 40.065 307 168 6 38 329 5 310 7.98e-65 214
Msa0640220 MtrunA17_Chr6g0465871 40.065 307 168 6 38 329 5 310 7.98e-65 214
Msa0640220 MtrunA17_Chr6g0466081 40.065 307 168 6 38 329 5 310 7.98e-65 214
Msa0640220 MtrunA17_Chr7g0237011 29.303 488 261 6 96 503 13 496 1.50e-64 217
Msa0640220 MtrunA17_Chr7g0236961 29.098 488 262 6 96 503 13 496 9.79e-64 215
Msa0640220 MtrunA17_Chr5g0431571 29.020 541 299 10 46 503 9 547 1.14e-61 211
Msa0640220 MtrunA17_Chr4g0048911 28.283 495 262 9 76 484 54 541 5.36e-61 209
Msa0640220 MtrunA17_Chr6g0466111 41.392 273 146 4 70 329 4 275 1.14e-60 202
Msa0640220 MtrunA17_Chr6g0465931 41.392 273 146 4 70 329 4 275 1.32e-60 202
Msa0640220 MtrunA17_Chr4g0008621 32.195 410 222 11 45 402 20 425 5.44e-57 196
Msa0640220 MtrunA17_Chr7g0237031 41.463 246 133 3 96 331 13 257 1.27e-56 191
Msa0640220 MtrunA17_Chr7g0236951 41.057 246 134 3 96 331 13 257 7.85e-56 189
Msa0640220 MtrunA17_Chr8g0357221 40.784 255 137 5 32 272 36 290 2.80e-53 181
Msa0640220 MtrunA17_Chr2g0280581 28.429 503 264 14 70 480 2 500 1.58e-50 180
Msa0640220 MtrunA17_Chr4g0048921 28.099 484 255 11 86 484 77 552 1.05e-49 179
Msa0640220 MtrunA17_Chr3g0108941 28.800 500 262 15 70 480 2 496 3.12e-48 174
Msa0640220 MtrunA17_Chr3g0109001 28.600 500 263 15 70 480 2 496 1.54e-46 169
Msa0640220 MtrunA17_Chr3g0108981 29.487 468 243 11 98 480 6 471 2.17e-46 168
Msa0640220 MtrunA17_Chr3g0108961 29.487 468 243 11 98 480 6 471 2.17e-46 168
Msa0640220 MtrunA17_Chr6g0477111 29.362 470 240 14 98 480 6 470 2.98e-46 168
Msa0640220 MtrunA17_Chr3g0108991 28.169 497 268 14 70 480 2 495 3.05e-46 169
Msa0640220 MtrunA17_Chr3g0108951 28.169 497 268 14 70 480 2 495 3.05e-46 169
Msa0640220 MtrunA17_Chr3g0109031 27.944 501 264 14 70 480 2 495 6.18e-46 167
Msa0640220 MtrunA17_Chr3g0109011 29.274 468 243 13 98 480 6 470 6.15e-45 164
Msa0640220 MtrunA17_Chr2g0319071 29.424 486 266 13 76 504 23 488 1.21e-44 164
Msa0640220 MtrunA17_Chr3g0108971 29.060 468 244 13 98 480 6 470 2.07e-44 163
Msa0640220 MtrunA17_Chr3g0109021 27.944 501 264 15 70 480 2 495 2.12e-44 163
Msa0640220 MtrunA17_Chr3g0105691 27.634 503 267 14 68 480 33 528 1.20e-43 162
Msa0640220 MtrunA17_Chr7g0236971 27.047 403 218 5 173 503 1 399 1.81e-42 155
Msa0640220 MtrunA17_Chr3g0099031 30.326 399 229 9 70 421 2 398 5.45e-42 157
Msa0640220 MtrunA17_Chr6g0477121 29.484 407 208 11 150 480 10 413 4.92e-38 144
Msa0640220 MtrunA17_Chr7g0237071 40.000 190 104 2 96 275 13 202 6.73e-36 133
Msa0640220 MtrunA17_Chr5g0445511 41.212 165 94 2 169 331 2 165 3.13e-35 132
Msa0640220 MtrunA17_Chr7g0217361 29.236 301 144 5 270 502 8 307 1.92e-33 129
Msa0640220 MtrunA17_Chr2g0310121 28.000 375 205 9 96 407 276 648 7.84e-33 133
Msa0640220 MtrunA17_Chr6g0466021 40.217 184 97 4 38 208 5 188 5.63e-32 121
Msa0640220 MtrunA17_Chr3g0101681 26.702 382 216 8 96 415 277 656 1.97e-31 129
Msa0640220 MtrunA17_Chr8g0357231 32.558 258 105 7 314 503 1 257 2.88e-29 115
Msa0640220 MtrunA17_Chr7g0217351 41.146 192 95 7 96 273 86 273 3.03e-29 117
Msa0640220 MtrunA17_Chr7g0218001 26.271 354 186 8 98 379 349 699 6.07e-24 105
Msa0640220 MtrunA17_Chr7g0259881 28.000 275 147 8 98 329 232 498 4.07e-23 103
Msa0640220 MtrunA17_Chr2g0319081 25.172 290 154 8 273 503 140 425 1.34e-19 91.3
Msa0640220 MtrunA17_Chr2g0310411 42.202 109 55 4 46 148 58 164 1.57e-18 83.2
Msa0640220 MtrunA17_Chr7g0233861 33.613 119 69 1 213 331 35 143 3.22e-15 73.9
Msa0640220 MtrunA17_Chr7g0218041 30.769 221 114 9 135 324 1 213 3.23e-15 77.8
Msa0640220 MtrunA17_Chr5g0411111 32.520 123 69 3 135 244 1 122 1.42e-13 67.8
Msa0640220 MtrunA17_Chr5g0445401 43.617 94 46 2 186 275 4 94 1.99e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0640220 AT3G25820.1 35.727 557 262 13 39 503 40 592 7.57e-107 331
Msa0640220 AT3G25830.1 35.727 557 262 13 39 503 40 592 7.57e-107 331
Msa0640220 AT3G25810.1 34.991 563 249 14 45 503 46 595 6.52e-101 315
Msa0640220 AT2G24210.1 33.935 554 273 13 39 503 39 588 4.54e-100 313
Msa0640220 AT4G16740.1 34.679 545 261 12 46 503 26 562 1.21e-98 308
Msa0640220 AT4G16730.1 31.648 534 273 10 61 503 5 537 1.05e-90 287
Msa0640220 AT4G16730.2 32.520 492 245 8 98 503 1 491 3.26e-88 279
Msa0640220 AT3G25820.2 38.243 387 184 8 39 374 40 422 1.91e-77 250
Msa0640220 AT4G16740.2 38.798 366 164 8 46 358 26 384 4.49e-71 232
Msa0640220 AT3G25820.4 39.589 341 176 9 39 358 40 371 6.83e-70 229
Msa0640220 AT3G25820.3 39.589 341 176 9 39 358 40 371 6.83e-70 229
Msa0640220 AT4G16740.4 42.532 308 148 7 46 331 26 326 3.59e-68 223
Msa0640220 AT4G16740.3 42.532 308 148 7 46 331 26 326 3.59e-68 223
Msa0640220 AT5G23960.2 27.593 540 306 10 46 503 7 543 2.55e-58 202
Msa0640220 AT5G23960.1 27.491 542 306 10 46 503 7 545 6.99e-58 201
Msa0640220 AT1G70080.1 28.287 502 260 11 94 504 118 610 3.39e-51 184
Msa0640220 AT1G61680.1 27.311 476 266 7 96 493 94 567 1.63e-50 181
Msa0640220 AT1G61680.2 26.681 476 262 8 96 493 94 560 3.46e-47 172
Msa0640220 AT3G29410.2 25.979 485 281 7 96 503 13 496 3.67e-47 171
Msa0640220 AT4G15870.1 29.424 469 271 15 6 428 30 484 5.61e-47 172
Msa0640220 AT3G29410.1 24.818 548 328 10 38 503 56 601 1.18e-46 171
Msa0640220 AT5G44630.1 32.642 386 210 11 89 428 63 444 8.94e-46 168
Msa0640220 AT4G20230.2 27.430 463 256 8 105 488 138 599 1.41e-45 169
Msa0640220 AT4G20230.1 27.430 463 256 8 105 488 126 587 1.49e-45 169
Msa0640220 AT3G14540.2 27.828 442 260 12 38 427 52 486 4.74e-45 166
Msa0640220 AT3G14490.1 29.398 432 247 10 11 388 21 448 6.51e-45 167
Msa0640220 AT3G14540.1 27.828 442 260 12 38 427 52 486 1.80e-44 166
Msa0640220 AT3G14520.1 25.045 555 320 14 38 503 55 602 2.97e-43 162
Msa0640220 AT1G31950.1 24.816 544 315 14 48 503 67 604 2.23e-42 160
Msa0640220 AT1G31950.3 25.767 489 280 11 96 503 119 605 3.47e-42 159
Msa0640220 AT1G33750.1 25.000 576 345 13 12 503 29 601 5.06e-42 159
Msa0640220 AT1G31950.2 28.191 376 226 8 96 428 119 493 6.35e-42 157
Msa0640220 AT4G13300.1 29.219 397 231 11 80 428 46 440 2.92e-41 155
Msa0640220 AT4G20200.1 26.113 494 285 9 90 504 111 603 2.56e-40 154
Msa0640220 AT3G29190.1 25.152 493 289 8 90 503 108 599 5.47e-40 153
Msa0640220 AT1G48820.1 29.039 458 257 16 3 422 17 444 7.48e-40 152
Msa0640220 AT4G20210.1 26.545 550 303 15 46 503 58 598 4.62e-39 150
Msa0640220 AT4G13280.2 29.041 365 212 9 108 428 79 440 3.49e-38 147
Msa0640220 AT1G48800.1 28.770 431 243 12 46 420 63 485 5.87e-38 147
Msa0640220 AT1G66020.1 25.709 494 287 9 90 504 105 597 5.98e-37 144
Msa0640220 AT3G32030.1 25.847 472 291 13 37 457 54 517 8.94e-36 141
Msa0640220 AT2G23230.2 23.726 569 346 12 23 504 32 599 1.11e-35 140
Msa0640220 AT2G23230.1 23.902 569 340 13 23 504 32 594 1.47e-35 140
Msa0640220 AT4G13280.1 32.881 295 174 7 108 381 79 370 1.02e-34 137
Msa0640220 AT1G66020.2 33.865 251 154 4 90 329 105 354 1.60e-33 132
Msa0640220 AT3G29110.1 25.926 459 289 9 25 434 34 490 4.15e-33 133
Msa0640220 AT1G79460.1 28.533 375 202 12 96 407 275 646 8.13e-27 115
Msa0640220 AT4G02780.1 32.331 266 137 10 98 324 334 595 1.30e-22 102
Msa0640220 AT1G61120.1 26.168 321 179 8 126 397 329 640 2.97e-21 98.2
Msa0640220 AT5G48110.1 25.764 229 137 4 231 428 2 228 6.88e-18 85.5

Find 102 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTACCAACCCAATATT+TGG 0.147243 4_3:-38136160 Msa0640220:CDS
CCAAATATTGGGTTGGTAAT+TGG 0.157698 4_3:+38136160 None:intergenic
ATGAAACTGCCTTAAGATTT+AGG 0.186680 4_3:-38135795 Msa0640220:CDS
AATTGTTTGAATTAATTAAT+TGG 0.198474 4_3:+38132003 None:intergenic
CGATTCTTTGCCATGGTATC+AGG 0.198740 4_3:+38132048 None:intergenic
TTGAGGTAGTCATCAAATTT+TGG 0.199877 4_3:+38132593 None:intergenic
GATATCGTAATTCCAAATAT+TGG 0.205792 4_3:+38136148 None:intergenic
TTATGAAGGAGAACAAATTT+TGG 0.222041 4_3:-38134702 Msa0640220:CDS
CACAATGTTGAAATTCAATT+TGG 0.223651 4_3:+38134490 None:intergenic
TAAGGATACTTATGTTATTA+TGG 0.250126 4_3:+38136341 None:intergenic
GGCTAATGGAATGTTTCTTT+TGG 0.253920 4_3:-38133771 Msa0640220:CDS
ATCATAAATGTCATCAATTA+AGG 0.255486 4_3:+38133672 None:intergenic
GAAACTAGGAAGTGAGTTAA+AGG 0.269615 4_3:+38136289 None:intergenic
TTAACATTCCTTTGATATCT+TGG 0.275349 4_3:+38134750 None:intergenic
GGAAGTAAGAAGAATGATTA+AGG 0.279467 4_3:-38135965 Msa0640220:CDS
TTTAGGCTCCTTCGACAATA+TGG 0.303451 4_3:-38135778 Msa0640220:CDS
ATATCGTAATTCCAAATATT+GGG 0.331518 4_3:+38136149 None:intergenic
AACATTGGATGAATTGTATA+AGG 0.332260 4_3:+38136323 None:intergenic
GTATATGAAGATATTCTTCT+TGG 0.341174 4_3:-38133370 Msa0640220:intron
TGCAGTGGTTCAAACAATAT+TGG 0.363548 4_3:-38135829 Msa0640220:intron
TACCTTCACTAAGTTCTTTG+AGG 0.366233 4_3:+38134651 None:intergenic
AAAGGAGGGGCTTGAGTACT+TGG 0.380341 4_3:-38132498 Msa0640220:CDS
CTCCATGACCATCTCCATAT+TGG 0.381267 4_3:+38132073 None:intergenic
TCTCACTGCACGTACCAATA+TGG 0.388938 4_3:-38132087 Msa0640220:CDS
AGATAGGAGAAGCCTTTGAT+AGG 0.389550 4_3:-38135870 Msa0640220:intron
TGATCAAGTGAATCATTCAT+TGG 0.390845 4_3:-38134612 Msa0640220:CDS
CATGGCAAAGAATCGAATAA+AGG 0.400991 4_3:-38132040 Msa0640220:CDS
ACCATCGAGTTTCAAGACTT+TGG 0.414873 4_3:+38134567 None:intergenic
TATGACACACTTAAAGAAAC+AGG 0.415257 4_3:-38133318 Msa0640220:CDS
GGTAATTAGAGAGGAAACTT+TGG 0.415262 4_3:+38133693 None:intergenic
CACGACCAAAGCTTAACCTT+GGG 0.417065 4_3:+38133797 None:intergenic
ACATATGAAGGTGGTGGAAG+GGG 0.417594 4_3:-38133831 Msa0640220:intron
GGTTAAGCTTTGGTCGTGAT+AGG 0.419053 4_3:-38133792 Msa0640220:CDS
CTTTGGTCGTGATAGGCTAA+TGG 0.425070 4_3:-38133785 Msa0640220:CDS
TACCGCATAATCGTGTTTCA+AGG 0.426734 4_3:+38136118 None:intergenic
TATCAATTCGAAAAGGAGAT+AGG 0.437146 4_3:-38135886 Msa0640220:CDS
GACGGTCTCTGCAACAATAT+AGG 0.442085 4_3:+38132467 None:intergenic
TCACTAAACTGAGGCTCAAA+TGG 0.444620 4_3:+38133733 None:intergenic
TGTGCAATCAACTCTTCAAC+AGG 0.451044 4_3:-38134471 Msa0640220:CDS
GAAGTGGAGAACCATTTCTA+TGG 0.461629 4_3:+38136228 None:intergenic
ATGAAGGTGGTGGAAGGGGA+TGG 0.461912 4_3:-38133827 Msa0640220:intron
AATAATCTCAGCCATAGAAA+TGG 0.464760 4_3:-38136239 Msa0640220:CDS
TCGAAGGAGCCTAAATCTTA+AGG 0.477296 4_3:+38135786 None:intergenic
CTAGCCCCAAGGTTAAGCTT+TGG 0.484468 4_3:-38133802 Msa0640220:CDS
GATTCTTTGCCATGGTATCA+GGG 0.502496 4_3:+38132049 None:intergenic
TGAAGGTGGTGGAAGGGGAT+GGG 0.505139 4_3:-38133826 Msa0640220:intron
AATGCATGGGTGTCTGTCTC+CGG 0.505741 4_3:-38132569 Msa0640220:CDS
GCATCATTTCTATCTTATGA+AGG 0.509979 4_3:-38134716 Msa0640220:CDS
AAGGAAGAGTTCAACAACAT+TGG 0.512213 4_3:+38136308 None:intergenic
CTTCACTAAGTTCTTTGAGG+TGG 0.519302 4_3:+38134654 None:intergenic
TTCTTCAAATCTCAAGAACT+AGG 0.521875 4_3:-38136269 Msa0640220:CDS
CTTTATTCGATTCTTTGCCA+TGG 0.524173 4_3:+38132041 None:intergenic
CGTAATTCCAAATATTGGGT+TGG 0.529017 4_3:+38136153 None:intergenic
TCTTGAGATTTGAAGAAACT+AGG 0.535631 4_3:+38136275 None:intergenic
TACATATGAAGGTGGTGGAA+GGG 0.538647 4_3:-38133832 Msa0640220:intron
TCACGACCAAAGCTTAACCT+TGG 0.543926 4_3:+38133796 None:intergenic
TCTCAGTTATCAATTCGAAA+AGG 0.545405 4_3:-38135893 Msa0640220:CDS
TTACATATGAAGGTGGTGGA+AGG 0.555487 4_3:-38133833 Msa0640220:intron
ATCAATTAAGGTAATTAGAG+AGG 0.557226 4_3:+38133684 None:intergenic
CTCGATACGTGGATAGGAGT+AGG 0.559477 4_3:-38135999 Msa0640220:CDS
TCATGGAGCCCCTGATACCA+TGG 0.560232 4_3:-38132058 Msa0640220:CDS
TATGGTTATGATGTTTCACC+AGG 0.562009 4_3:-38135760 Msa0640220:intron
TAGGAGTAGGAGGATGCAAG+AGG 0.567410 4_3:-38135986 Msa0640220:CDS
CTTTGGACTCTACGATGTAG+TGG 0.570277 4_3:+38134584 None:intergenic
TCAGTTTAGTGATATGCGCA+AGG 0.571438 4_3:-38133722 Msa0640220:CDS
ATGACTACCTCAACAATGCA+TGG 0.572804 4_3:-38132583 Msa0640220:CDS
AATGATTCACTTGATCAAAG+AGG 0.573638 4_3:+38134618 None:intergenic
TCCAAAGTCTTGAAACTCGA+TGG 0.575055 4_3:-38134568 Msa0640220:CDS
TTAGTAGCGTAACATAGAAG+TGG 0.576392 4_3:+38136212 None:intergenic
CATTTCTACAAGAAGCAAGA+TGG 0.579905 4_3:-38132634 Msa0640220:CDS
AATCGTAACATAACAAAGGA+GGG 0.583261 4_3:-38132512 Msa0640220:CDS
CAGTTTAGTGATATGCGCAA+GGG 0.586945 4_3:-38133721 Msa0640220:CDS
AGGATGCTCGATACGTGGAT+AGG 0.588378 4_3:-38136005 Msa0640220:CDS
ATCGATACTGTGAAACACTT+AGG 0.593154 4_3:-38135916 Msa0640220:CDS
ATCCTTGAAACACGATTATG+CGG 0.595568 4_3:-38136120 Msa0640220:intron
CATCATAACCATATTGTCGA+AGG 0.601484 4_3:+38135770 None:intergenic
TCATACCGCTTTGACAAGGT+AGG 0.603491 4_3:+38133281 None:intergenic
ATCGTAACATAACAAAGGAG+GGG 0.606500 4_3:-38132511 Msa0640220:CDS
GCTAAATCGTAACATAACAA+AGG 0.606945 4_3:-38132516 Msa0640220:CDS
ATTCTTTGCCATGGTATCAG+GGG 0.608231 4_3:+38132050 None:intergenic
AAATCGTAACATAACAAAGG+AGG 0.612370 4_3:-38132513 Msa0640220:CDS
CATACCGCTTTGACAAGGTA+GGG 0.613885 4_3:+38133282 None:intergenic
TGTCTTGTCCAAGATATCAA+AGG 0.617651 4_3:-38134758 Msa0640220:CDS
TTTCTAGGATGCTCGATACG+TGG 0.619384 4_3:-38136010 Msa0640220:intron
TACCAATATGGAGATGGTCA+TGG 0.623302 4_3:-38132075 Msa0640220:CDS
AGGGGATGGGACTAGCCCCA+AGG 0.624304 4_3:-38133813 Msa0640220:CDS
TGCACGTACCAATATGGAGA+TGG 0.629166 4_3:-38132081 Msa0640220:CDS
TCTTCCCTACCTTGTCAAAG+CGG 0.642377 4_3:-38133286 Msa0640220:intron
TGCAGTGAGAAATTCTAGCA+AGG 0.643300 4_3:+38132100 None:intergenic
AGGATCTTAAAGAAACGTCA+AGG 0.646273 4_3:-38134451 Msa0640220:intron
ATGCCTTAAAATCTCCGTTG+TGG 0.655650 4_3:+38134780 None:intergenic
TTGGTCATACCGCTTTGACA+AGG 0.662027 4_3:+38133277 None:intergenic
GATACGTGGATAGGAGTAGG+AGG 0.665547 4_3:-38135996 Msa0640220:CDS
GTGGATGCTAGTGAAAGCAT+TGG 0.666886 4_3:+38134673 None:intergenic
CAGACACCCATGCATTGTTG+AGG 0.669111 4_3:+38132576 None:intergenic
TGAAAATGGTGTTAGTGAGG+AGG 0.675695 4_3:-38132232 Msa0640220:intron
TGACTACCTCAACAATGCAT+GGG 0.680426 4_3:-38132582 Msa0640220:CDS
ATGGGTGAGTAAAACTACAC+CGG 0.689202 4_3:+38132550 None:intergenic
ACGACCAAAGCTTAACCTTG+GGG 0.696222 4_3:+38133798 None:intergenic
TTGCGCATATCACTAAACTG+AGG 0.729129 4_3:+38133724 None:intergenic
GAGATGTTTAAGGACCACAA+CGG 0.742734 4_3:-38134794 Msa0640220:CDS
AGGAATGTTAAGTCTCTACG+AGG 0.767381 4_3:-38134738 Msa0640220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTTTGTATTATTTCT+AGG - chr4_3:38132374-38132393 Msa0640220:intron 10.0%
!!! TTTTTTTTAAGAATCAAAAA+TGG + chr4_3:38133167-38133186 None:intergenic 10.0%
!!! TTTTAAAATCTTTTTTTTTG+TGG + chr4_3:38133380-38133399 None:intergenic 10.0%
!!! TTTTTTCTTATAAATATAAC+CGG + chr4_3:38135334-38135353 None:intergenic 10.0%
!!! ATTTTTTTTTCCAACTAATA+TGG + chr4_3:38132723-38132742 None:intergenic 15.0%
!!! AAAATGGATTATATAAACAA+CGG + chr4_3:38133151-38133170 None:intergenic 15.0%
!!! ATATTGATTCATATGAAAAA+AGG - chr4_3:38133855-38133874 Msa0640220:intron 15.0%
!! TTATTTCATTTACAAATTTG+AGG + chr4_3:38134285-38134304 None:intergenic 15.0%
!!! TTTTTTATAAGCTATACAAT+TGG + chr4_3:38134468-38134487 None:intergenic 15.0%
!!! TATTTTTCTTTTACATATGA+AGG - chr4_3:38134556-38134575 Msa0640220:CDS 15.0%
!! AAAATTATGTTTATTTCAGT+TGG - chr4_3:38134977-38134996 Msa0640220:intron 15.0%
!! AAATTATGTTTATTTCAGTT+GGG - chr4_3:38134978-38134997 Msa0640220:intron 15.0%
!!! TTTTTCTTATAAATATAACC+GGG + chr4_3:38135333-38135352 None:intergenic 15.0%
!!! TAAGAAAAAAGCAATTTTTT+AGG - chr4_3:38135344-38135363 Msa0640220:intron 15.0%
!!! ATTTAGCTTATAATTATGAT+TGG - chr4_3:38135386-38135405 Msa0640220:intron 15.0%
!! TAAGGATACTTATGTTATTA+TGG + chr4_3:38132061-38132080 None:intergenic 20.0%
!! ATATCGTAATTCCAAATATT+GGG + chr4_3:38132253-38132272 None:intergenic 20.0%
!!! TTTTTTGTCAAATAGTTTAG+TGG - chr4_3:38132314-38132333 Msa0640220:intron 20.0%
!! AAAAGATAAAAACCTATCAA+AGG + chr4_3:38132544-38132563 None:intergenic 20.0%
!! ATTATTCTTACCATATTAGT+TGG - chr4_3:38132710-38132729 Msa0640220:intron 20.0%
!!! TTCATGATATATATTTTCAC+TGG - chr4_3:38132851-38132870 Msa0640220:intron 20.0%
!! ATATTTAGGTAAGGTTATAA+TGG + chr4_3:38133314-38133333 None:intergenic 20.0%
!!! AGATGTTTTTCTATATGATT+AGG - chr4_3:38133539-38133558 Msa0640220:intron 20.0%
!!! AGATATTTTTGAGATGTTTA+AGG - chr4_3:38133595-38133614 Msa0640220:intron 20.0%
!! CTTATATTAGATGGAAAAAT+TGG + chr4_3:38134131-38134150 None:intergenic 20.0%
!! AATATGTTATGTGAGTAAAA+GGG + chr4_3:38134155-38134174 None:intergenic 20.0%
!! AAATATGTTATGTGAGTAAA+AGG + chr4_3:38134156-38134175 None:intergenic 20.0%
!! GATATGCTAAAAAAAACATA+TGG + chr4_3:38134402-38134421 None:intergenic 20.0%
!! ATCATAAATGTCATCAATTA+AGG + chr4_3:38134730-38134749 None:intergenic 20.0%
!!! CTTTTTTATAAGAGAAAGTT+TGG - chr4_3:38134825-38134844 Msa0640220:intron 20.0%
!! GAATGAATTATAAATAAGAC+CGG - chr4_3:38134870-38134889 Msa0640220:intron 20.0%
!! AATGAATTATAAATAAGACC+GGG - chr4_3:38134871-38134890 Msa0640220:intron 20.0%
!! CAGCTAAATAATTTAGATTT+AGG - chr4_3:38135281-38135300 Msa0640220:intron 20.0%
!!! TTTTCTTATAAATATAACCG+GGG + chr4_3:38135332-38135351 None:intergenic 20.0%
!!! TTTTTGCTTATAAATGTGAT+TGG - chr4_3:38135443-38135462 Msa0640220:intron 20.0%
!!! AATCCATCAAATTTTTCATT+TGG - chr4_3:38135668-38135687 Msa0640220:intron 20.0%
!! GTTATACTAATATATAGACA+TGG - chr4_3:38135704-38135723 Msa0640220:intron 20.0%
!! ATTTAGCAAAAAATAGGAAT+GGG + chr4_3:38135870-38135889 None:intergenic 20.0%
!!! AAGTTTCATTAAGCAATTTT+TGG + chr4_3:38136209-38136228 None:intergenic 20.0%
!! AAAAATTGCTTAATGAAACT+TGG - chr4_3:38136208-38136227 Msa0640220:CDS 20.0%
!!! TTTTTTGCAAGTGAGAATAA+GGG + chr4_3:38132033-38132052 None:intergenic 25.0%
! AACATTGGATGAATTGTATA+AGG + chr4_3:38132079-38132098 None:intergenic 25.0%
! GATATCGTAATTCCAAATAT+TGG + chr4_3:38132254-38132273 None:intergenic 25.0%
!!! TTATCTTTTGAAAAATGCAG+TGG - chr4_3:38132555-38132574 Msa0640220:CDS 25.0%
! AGGTTAAATTTACTTCTTTG+TGG - chr4_3:38132957-38132976 Msa0640220:intron 25.0%
! TTAAATTTACTTCTTTGTGG+TGG - chr4_3:38132960-38132979 Msa0640220:intron 25.0%
! GTGTATAAAACCAGAAATAA+AGG + chr4_3:38133067-38133086 None:intergenic 25.0%
!! ACACTGACAAATTGTAATTT+TGG - chr4_3:38133083-38133102 Msa0640220:intron 25.0%
! AATGGTACAATATTTAGGTA+AGG + chr4_3:38133323-38133342 None:intergenic 25.0%
! ACTACAATGGTACAATATTT+AGG + chr4_3:38133328-38133347 None:intergenic 25.0%
! CTGTTTATCATATACTACAA+TGG + chr4_3:38133341-38133360 None:intergenic 25.0%
! CATTGTAGTATATGATAAAC+AGG - chr4_3:38133339-38133358 Msa0640220:CDS 25.0%
!! GAGTTCTAATAAAATTGTGA+GGG + chr4_3:38133408-38133427 None:intergenic 25.0%
!! TGAGTTCTAATAAAATTGTG+AGG + chr4_3:38133409-38133428 None:intergenic 25.0%
!!! TAGATTTCTGAAACTGAAAA+TGG + chr4_3:38133456-38133475 None:intergenic 25.0%
! AAAAAAGATCTTCATCATTG+TGG + chr4_3:38133566-38133585 None:intergenic 25.0%
! TTAACATTCCTTTGATATCT+TGG + chr4_3:38133652-38133671 None:intergenic 25.0%
!! TTATGAAGGAGAACAAATTT+TGG - chr4_3:38133697-38133716 Msa0640220:CDS 25.0%
! TGATTCATATGAAAAAAGGA+AGG - chr4_3:38133859-38133878 Msa0640220:intron 25.0%
! CACAATGTTGAAATTCAATT+TGG + chr4_3:38133912-38133931 None:intergenic 25.0%
!! TGAGAATGATTTCATTTTGT+TGG - chr4_3:38133985-38134004 Msa0640220:intron 25.0%
!! GAGAATGATTTCATTTTGTT+GGG - chr4_3:38133986-38134005 Msa0640220:intron 25.0%
! ATCATCATCAAATTAGATCT+TGG - chr4_3:38134029-38134048 Msa0640220:intron 25.0%
! TCATCATCAAATTAGATCTT+GGG - chr4_3:38134030-38134049 Msa0640220:intron 25.0%
! CTCAAAACTATAATGTCTTA+AGG + chr4_3:38134081-38134100 None:intergenic 25.0%
!! TAAAAGGGTCTTATATTAGA+TGG + chr4_3:38134140-38134159 None:intergenic 25.0%
!! ATAACATATTTTCACTTGTG+TGG - chr4_3:38134165-38134184 Msa0640220:intron 25.0%
! GTATAATAGGGTAGAAAAAA+AGG + chr4_3:38134446-38134465 None:intergenic 25.0%
! GCTATACAATTGGTATAATA+GGG + chr4_3:38134458-38134477 None:intergenic 25.0%
! AGCTATACAATTGGTATAAT+AGG + chr4_3:38134459-38134478 None:intergenic 25.0%
!! TTTTCTTTTACATATGAAGG+TGG - chr4_3:38134559-38134578 Msa0640220:CDS 25.0%
! ATCAATTAAGGTAATTAGAG+AGG + chr4_3:38134718-38134737 None:intergenic 25.0%
! GATGACATTTATGATGTTTA+TGG - chr4_3:38134735-38134754 Msa0640220:CDS 25.0%
! CTTTCTCTTATAAAAAAGAC+TGG + chr4_3:38134823-38134842 None:intergenic 25.0%
! TCAACAAAAGTTTATGTATC+TGG - chr4_3:38134848-38134867 Msa0640220:intron 25.0%
! TAATGCAATTCAAATTCTTC+CGG - chr4_3:38135005-38135024 Msa0640220:intron 25.0%
! AAGAATATCTTCATATACTC+CGG + chr4_3:38135027-38135046 None:intergenic 25.0%
! GTATATGAAGATATTCTTCT+TGG - chr4_3:38135029-38135048 Msa0640220:intron 25.0%
!! ACATGTTTAAAACATGTCTT+AGG - chr4_3:38135180-38135199 Msa0640220:intron 25.0%
!! CATGTTTAAAACATGTCTTA+GGG - chr4_3:38135181-38135200 Msa0640220:intron 25.0%
! AATTTAGATTTAGGTACACA+TGG - chr4_3:38135290-38135309 Msa0640220:intron 25.0%
! TTTCTTATAAATATAACCGG+GGG + chr4_3:38135331-38135350 None:intergenic 25.0%
!! TCATACCCATCAAATTTTAA+TGG - chr4_3:38135499-38135518 Msa0640220:intron 25.0%
!!! CATACCCATCAAATTTTAAT+GGG - chr4_3:38135500-38135519 Msa0640220:intron 25.0%
!!! ATACCCATCAAATTTTAATG+GGG - chr4_3:38135501-38135520 Msa0640220:intron 25.0%
! ACACCAAATGAAAAATTTGA+TGG + chr4_3:38135674-38135693 None:intergenic 25.0%
!! GTTTTTCCAATAAATTACAG+TGG - chr4_3:38135726-38135745 Msa0640220:intron 25.0%
! GATTTAGCAAAAAATAGGAA+TGG + chr4_3:38135871-38135890 None:intergenic 25.0%
! GTTACGATTTAGCAAAAAAT+AGG + chr4_3:38135876-38135895 None:intergenic 25.0%
! ATTGCAAAGTCGAAAAATTA+TGG + chr4_3:38135957-38135976 None:intergenic 25.0%
! ATAATATGCATGTATGGTTA+AGG + chr4_3:38136017-38136036 None:intergenic 25.0%
! TGTAAGATAATATGCATGTA+TGG + chr4_3:38136023-38136042 None:intergenic 25.0%
! TGTGATCTATATAACTTTGT+AGG - chr4_3:38136095-38136114 Msa0640220:intron 25.0%
! ATATGCTACATGAATGAAAA+TGG - chr4_3:38136153-38136172 Msa0640220:CDS 25.0%
!!! TGTCTCTGAAAAATATTTTG+AGG + chr4_3:38136271-38136290 None:intergenic 25.0%
!!! GTTTTTTGCAAGTGAGAATA+AGG + chr4_3:38132034-38132053 None:intergenic 30.0%
TCTTGAGATTTGAAGAAACT+AGG + chr4_3:38132127-38132146 None:intergenic 30.0%
TTCTTCAAATCTCAAGAACT+AGG - chr4_3:38132130-38132149 Msa0640220:CDS 30.0%
AATAATCTCAGCCATAGAAA+TGG - chr4_3:38132160-38132179 Msa0640220:CDS 30.0%
GGAAGTAAGAAGAATGATTA+AGG - chr4_3:38132434-38132453 Msa0640220:intron 30.0%
TCTCAGTTATCAATTCGAAA+AGG - chr4_3:38132506-38132525 Msa0640220:CDS 30.0%
TATCAATTCGAAAAGGAGAT+AGG - chr4_3:38132513-38132532 Msa0640220:CDS 30.0%
ATGAAACTGCCTTAAGATTT+AGG - chr4_3:38132604-38132623 Msa0640220:CDS 30.0%
!!! AATAATGCCACTTTTGCATT+TGG - chr4_3:38132992-38133011 Msa0640220:intron 30.0%
! TATCAACTGACCTTTATTTC+TGG - chr4_3:38133054-38133073 Msa0640220:intron 30.0%
GCATCATTTCTATCTTATGA+AGG - chr4_3:38133683-38133702 Msa0640220:CDS 30.0%
AATGATTCACTTGATCAAAG+AGG + chr4_3:38133784-38133803 None:intergenic 30.0%
TGATCAAGTGAATCATTCAT+TGG - chr4_3:38133787-38133806 Msa0640220:CDS 30.0%
GATGTTGGACACTATATAAA+TGG + chr4_3:38134109-38134128 None:intergenic 30.0%
TAGATGGAAAAATTGGATGT+TGG + chr4_3:38134124-38134143 None:intergenic 30.0%
! GCTAATGGAATGTTTCTTTT+GGG - chr4_3:38134629-38134648 Msa0640220:CDS 30.0%
! AAAAAGCTCTAATTCTTCCA+AGG + chr4_3:38134763-38134782 None:intergenic 30.0%
! ATTAGAGCTTTTTACAACTG+CGG - chr4_3:38134770-38134789 Msa0640220:CDS 30.0%
CTCTTATAAAAAAGACTGGA+GGG + chr4_3:38134819-38134838 None:intergenic 30.0%
TCTCTTATAAAAAAGACTGG+AGG + chr4_3:38134820-38134839 None:intergenic 30.0%
TATGACACACTTAAAGAAAC+AGG - chr4_3:38135081-38135100 Msa0640220:intron 30.0%
TAAAAACCACGTTCACAAAA+TGG - chr4_3:38135152-38135171 Msa0640220:intron 30.0%
CGTAATTAACTGTTAAGAAG+TGG + chr4_3:38135549-38135568 None:intergenic 30.0%
TAAAAAAACCATGCAAGTCT+TGG + chr4_3:38135607-38135626 None:intergenic 30.0%
!!! ACTTGCATGGTTTTTTTAAC+TGG - chr4_3:38135609-38135628 Msa0640220:intron 30.0%
ATCAGACCACTGTAATTTAT+TGG + chr4_3:38135735-38135754 None:intergenic 30.0%
! TTGAGGTAGTCATCAAATTT+TGG + chr4_3:38135809-38135828 None:intergenic 30.0%
GCTAAATCGTAACATAACAA+AGG - chr4_3:38135883-38135902 Msa0640220:CDS 30.0%
AAATCGTAACATAACAAAGG+AGG - chr4_3:38135886-38135905 Msa0640220:CDS 30.0%
AATCGTAACATAACAAAGGA+GGG - chr4_3:38135887-38135906 Msa0640220:CDS 30.0%
CAAAGTCGAAAAATTATGGA+CGG + chr4_3:38135953-38135972 None:intergenic 30.0%
! TTTTCGACTTTGCAATGATT+TGG - chr4_3:38135961-38135980 Msa0640220:CDS 30.0%
GATCTATATAACTTTGTAGG+CGG - chr4_3:38136098-38136117 Msa0640220:intron 30.0%
!!! TCTGAAAAATATTTTGAGGC+AGG + chr4_3:38136267-38136286 None:intergenic 30.0%
AAGGAAGAGTTCAACAACAT+TGG + chr4_3:38132094-38132113 None:intergenic 35.0%
GAAACTAGGAAGTGAGTTAA+AGG + chr4_3:38132113-38132132 None:intergenic 35.0%
TTAGTAGCGTAACATAGAAG+TGG + chr4_3:38132190-38132209 None:intergenic 35.0%
!! CCAAATATTGGGTTGGTAAT+TGG + chr4_3:38132242-38132261 None:intergenic 35.0%
CCAATTACCAACCCAATATT+TGG - chr4_3:38132239-38132258 Msa0640220:intron 35.0%
CGTAATTCCAAATATTGGGT+TGG + chr4_3:38132249-38132268 None:intergenic 35.0%
ATCCTTGAAACACGATTATG+CGG - chr4_3:38132279-38132298 Msa0640220:intron 35.0%
ATCGATACTGTGAAACACTT+AGG - chr4_3:38132483-38132502 Msa0640220:CDS 35.0%
TGCAGTGGTTCAAACAATAT+TGG - chr4_3:38132570-38132589 Msa0640220:CDS 35.0%
CATCATAACCATATTGTCGA+AGG + chr4_3:38132632-38132651 None:intergenic 35.0%
TATGGTTATGATGTTTCACC+AGG - chr4_3:38132639-38132658 Msa0640220:CDS 35.0%
TAACGTTCAAATGACCATGA+TGG - chr4_3:38132922-38132941 Msa0640220:intron 35.0%
TTGAGTACCAAATGCAAAAG+TGG + chr4_3:38133002-38133021 None:intergenic 35.0%
TTGCATTTGGTACTCAAAGA+TGG - chr4_3:38133005-38133024 Msa0640220:intron 35.0%
TAGTACAAGGTGTACTCAAA+AGG + chr4_3:38133114-38133133 None:intergenic 35.0%
TGTCTTGTCCAAGATATCAA+AGG - chr4_3:38133641-38133660 Msa0640220:intron 35.0%
TACCTTCACTAAGTTCTTTG+AGG + chr4_3:38133751-38133770 None:intergenic 35.0%
AGGATCTTAAAGAAACGTCA+AGG - chr4_3:38133948-38133967 Msa0640220:intron 35.0%
TTTGGGAATAAAGTCGTGAT+CGG - chr4_3:38134246-38134265 Msa0640220:intron 35.0%
! TACAAATTTGAGGTGAATCG+TGG + chr4_3:38134275-38134294 None:intergenic 35.0%
! TCTTTTACATATGAAGGTGG+TGG - chr4_3:38134562-38134581 Msa0640220:CDS 35.0%
! GGCTAATGGAATGTTTCTTT+TGG - chr4_3:38134628-38134647 Msa0640220:CDS 35.0%
GGTAATTAGAGAGGAAACTT+TGG + chr4_3:38134709-38134728 None:intergenic 35.0%
TTATGATGTTTATGGCACCT+TGG - chr4_3:38134743-38134762 Msa0640220:CDS 35.0%
! TTTTTACAACTGCGGTAGAA+AGG - chr4_3:38134778-38134797 Msa0640220:CDS 35.0%
! ACGTGGTTTTTATGTGAGTT+TGG + chr4_3:38135144-38135163 None:intergenic 35.0%
! TTCTAACCATTTTGTGAACG+TGG + chr4_3:38135161-38135180 None:intergenic 35.0%
CATTTCTACAAGAAGCAAGA+TGG - chr4_3:38135765-38135784 Msa0640220:CDS 35.0%
ATCGTAACATAACAAAGGAG+GGG - chr4_3:38135888-38135907 Msa0640220:CDS 35.0%
! CAATGATTTGGCTACTTCAT+CGG - chr4_3:38135973-38135992 Msa0640220:CDS 35.0%
ATGCATGTATGGTTAAGGTA+AGG + chr4_3:38136012-38136031 None:intergenic 35.0%
! GAATGAAAATGGTGTTAGTG+AGG - chr4_3:38136164-38136183 Msa0640220:CDS 35.0%
CTTTATTCGATTCTTTGCCA+TGG + chr4_3:38136361-38136380 None:intergenic 35.0%
CATGGCAAAGAATCGAATAA+AGG - chr4_3:38136359-38136378 Msa0640220:five_prime_UTR 35.0%
GAAGTGGAGAACCATTTCTA+TGG + chr4_3:38132174-38132193 None:intergenic 40.0%
TACCGCATAATCGTGTTTCA+AGG + chr4_3:38132284-38132303 None:intergenic 40.0%
AGATAGGAGAAGCCTTTGAT+AGG - chr4_3:38132529-38132548 Msa0640220:CDS 40.0%
TCGAAGGAGCCTAAATCTTA+AGG + chr4_3:38132616-38132635 None:intergenic 40.0%
TTTAGGCTCCTTCGACAATA+TGG - chr4_3:38132621-38132640 Msa0640220:CDS 40.0%
! AATGACCATGATGGAACTGT+TGG - chr4_3:38132931-38132950 Msa0640220:intron 40.0%
! CTTTTCCAACAGTTCCATCA+TGG + chr4_3:38132939-38132958 None:intergenic 40.0%
! CATGATGGAACTGTTGGAAA+AGG - chr4_3:38132937-38132956 Msa0640220:intron 40.0%
TACTTCTTTGTGGTGGCTAA+AGG - chr4_3:38132967-38132986 Msa0640220:intron 40.0%
ATCGAACACGTTACAATGTC+TGG - chr4_3:38133030-38133049 Msa0640220:intron 40.0%
TCGAACACGTTACAATGTCT+GGG - chr4_3:38133031-38133050 Msa0640220:intron 40.0%
GAGTACACCTTGTACTAAAG+AGG - chr4_3:38133117-38133136 Msa0640220:intron 40.0%
AGTGAGTCCTCTTTAGTACA+AGG + chr4_3:38133127-38133146 None:intergenic 40.0%
GAGATGTTTAAGGACCACAA+CGG - chr4_3:38133605-38133624 Msa0640220:intron 40.0%
ATGCCTTAAAATCTCCGTTG+TGG + chr4_3:38133622-38133641 None:intergenic 40.0%
AGGAATGTTAAGTCTCTACG+AGG - chr4_3:38133661-38133680 Msa0640220:intron 40.0%
! CTTCACTAAGTTCTTTGAGG+TGG + chr4_3:38133748-38133767 None:intergenic 40.0%
CACCTCAAAGAACTTAGTGA+AGG - chr4_3:38133746-38133765 Msa0640220:CDS 40.0%
TCCAAAGTCTTGAAACTCGA+TGG - chr4_3:38133831-38133850 Msa0640220:intron 40.0%
ACCATCGAGTTTCAAGACTT+TGG + chr4_3:38133835-38133854 None:intergenic 40.0%
AAAAAGGAAGGACGCAAACA+AGG - chr4_3:38133871-38133890 Msa0640220:intron 40.0%
TGTGCAATCAACTCTTCAAC+AGG - chr4_3:38133928-38133947 Msa0640220:intron 40.0%
TAACTCCATTGGGACATGAT+GGG + chr4_3:38134223-38134242 None:intergenic 40.0%
ATAACTCCATTGGGACATGA+TGG + chr4_3:38134224-38134243 None:intergenic 40.0%
ATCATGTCCCAATGGAGTTA+TGG - chr4_3:38134223-38134242 Msa0640220:intron 40.0%
TCCCAATGGAGTTATGGTTT+GGG - chr4_3:38134229-38134248 Msa0640220:intron 40.0%
TCCCAAACCATAACTCCATT+GGG + chr4_3:38134233-38134252 None:intergenic 40.0%
TTCCCAAACCATAACTCCAT+TGG + chr4_3:38134234-38134253 None:intergenic 40.0%
TTGGGAATAAAGTCGTGATC+GGG - chr4_3:38134247-38134266 Msa0640220:intron 40.0%
!! TTACATATGAAGGTGGTGGA+AGG - chr4_3:38134566-38134585 Msa0640220:CDS 40.0%
!! TACATATGAAGGTGGTGGAA+GGG - chr4_3:38134567-38134586 Msa0640220:CDS 40.0%
TCACTAAACTGAGGCTCAAA+TGG + chr4_3:38134669-38134688 None:intergenic 40.0%
TTGCGCATATCACTAAACTG+AGG + chr4_3:38134678-38134697 None:intergenic 40.0%
TCAGTTTAGTGATATGCGCA+AGG - chr4_3:38134677-38134696 Msa0640220:CDS 40.0%
CAGTTTAGTGATATGCGCAA+GGG - chr4_3:38134678-38134697 Msa0640220:CDS 40.0%
TGCAAATAGTGTGATACTCC+CGG + chr4_3:38134892-38134911 None:intergenic 40.0%
GCGCCCCATTAAAATTTGAT+GGG + chr4_3:38135507-38135526 None:intergenic 40.0%
TGCGCCCCATTAAAATTTGA+TGG + chr4_3:38135508-38135527 None:intergenic 40.0%
!! TTTTCTAGCCAAGACTTGCA+TGG - chr4_3:38135596-38135615 Msa0640220:intron 40.0%
ATGACTACCTCAACAATGCA+TGG - chr4_3:38135816-38135835 Msa0640220:CDS 40.0%
TGACTACCTCAACAATGCAT+GGG - chr4_3:38135817-38135836 Msa0640220:CDS 40.0%
ATGGGTGAGTAAAACTACAC+CGG + chr4_3:38135852-38135871 None:intergenic 40.0%
! TGAAAATGGTGTTAGTGAGG+AGG - chr4_3:38136167-38136186 Msa0640220:CDS 40.0%
TGCAGTGAGAAATTCTAGCA+AGG + chr4_3:38136302-38136321 None:intergenic 40.0%
TACCAATATGGAGATGGTCA+TGG - chr4_3:38136324-38136343 Msa0640220:exon 40.0%
ATTCTTTGCCATGGTATCAG+GGG + chr4_3:38136352-38136371 None:intergenic 40.0%
GATTCTTTGCCATGGTATCA+GGG + chr4_3:38136353-38136372 None:intergenic 40.0%
!! TTTCTAGGATGCTCGATACG+TGG - chr4_3:38132389-38132408 Msa0640220:intron 45.0%
! GGACCACAACGGAGATTTTA+AGG - chr4_3:38133616-38133635 Msa0640220:intron 45.0%
! GTGGATGCTAGTGAAAGCAT+TGG + chr4_3:38133729-38133748 None:intergenic 45.0%
CTTTGGACTCTACGATGTAG+TGG + chr4_3:38133818-38133837 None:intergenic 45.0%
AAGCCTAATGTGATGCTCCT+CGG - chr4_3:38134195-38134214 Msa0640220:intron 45.0%
GTCCCAATGGAGTTATGGTT+TGG - chr4_3:38134228-38134247 Msa0640220:intron 45.0%
!! ACATATGAAGGTGGTGGAAG+GGG - chr4_3:38134568-38134587 Msa0640220:CDS 45.0%
ACGACCAAAGCTTAACCTTG+GGG + chr4_3:38134604-38134623 None:intergenic 45.0%
CACGACCAAAGCTTAACCTT+GGG + chr4_3:38134605-38134624 None:intergenic 45.0%
TCACGACCAAAGCTTAACCT+TGG + chr4_3:38134606-38134625 None:intergenic 45.0%
! GGTTAAGCTTTGGTCGTGAT+AGG - chr4_3:38134607-38134626 Msa0640220:CDS 45.0%
CTTTGGTCGTGATAGGCTAA+TGG - chr4_3:38134614-38134633 Msa0640220:CDS 45.0%
! GAATGTTTCTTTTGGGCTGC+TGG - chr4_3:38134636-38134655 Msa0640220:intron 45.0%
TCTTCCCTACCTTGTCAAAG+CGG - chr4_3:38135113-38135132 Msa0640220:intron 45.0%
CATACCGCTTTGACAAGGTA+GGG + chr4_3:38135120-38135139 None:intergenic 45.0%
TCATACCGCTTTGACAAGGT+AGG + chr4_3:38135121-38135140 None:intergenic 45.0%
TTGGTCATACCGCTTTGACA+AGG + chr4_3:38135125-38135144 None:intergenic 45.0%
! GTTGACATAAAGTACTCCCC+CGG - chr4_3:38135312-38135331 Msa0640220:intron 45.0%
GACGGTCTCTGCAACAATAT+AGG + chr4_3:38135935-38135954 None:intergenic 45.0%
TTGTAGGCGGAGTTAGAAAG+AGG - chr4_3:38136111-38136130 Msa0640220:intron 45.0%
TCTCACTGCACGTACCAATA+TGG - chr4_3:38136312-38136331 Msa0640220:exon 45.0%
TGCACGTACCAATATGGAGA+TGG - chr4_3:38136318-38136337 Msa0640220:exon 45.0%
CTCCATGACCATCTCCATAT+TGG + chr4_3:38136329-38136348 None:intergenic 45.0%
CGATTCTTTGCCATGGTATC+AGG + chr4_3:38136354-38136373 None:intergenic 45.0%
!!! TTATAAATTTGTAATTTTTT+AGG + chr4_3:38132800-38132819 None:intergenic 5.0%
!!! TAGATATTTTATAAAATTAA+CGG + chr4_3:38133272-38133291 None:intergenic 5.0%
!! AAGATATAATTTATAAAATT+TGG - chr4_3:38134487-38134506 Msa0640220:CDS 5.0%
!! AGATATAATTTATAAAATTT+GGG - chr4_3:38134488-38134507 Msa0640220:CDS 5.0%
!!! TTAATTTTTATTTATAACAA+TGG + chr4_3:38136046-38136065 None:intergenic 5.0%
AGGATGCTCGATACGTGGAT+AGG - chr4_3:38132394-38132413 Msa0640220:intron 50.0%
CTCGATACGTGGATAGGAGT+AGG - chr4_3:38132400-38132419 Msa0640220:intron 50.0%
GATACGTGGATAGGAGTAGG+AGG - chr4_3:38132403-38132422 Msa0640220:intron 50.0%
TAGGAGTAGGAGGATGCAAG+AGG - chr4_3:38132413-38132432 Msa0640220:intron 50.0%
TGTACGTATGCATGTGCACC+TGG + chr4_3:38132660-38132679 None:intergenic 50.0%
AGCCTAATGTGATGCTCCTC+GGG - chr4_3:38134196-38134215 Msa0640220:intron 50.0%
CTAGCCCCAAGGTTAAGCTT+TGG - chr4_3:38134597-38134616 Msa0640220:CDS 50.0%
CAGACACCCATGCATTGTTG+AGG + chr4_3:38135826-38135845 None:intergenic 50.0%
AATGCATGGGTGTCTGTCTC+CGG - chr4_3:38135830-38135849 Msa0640220:intron 50.0%
AAAGGAGGGGCTTGAGTACT+TGG - chr4_3:38135901-38135920 Msa0640220:CDS 50.0%
GCGGAGTTAGAAAGAGGTGA+AGG - chr4_3:38136117-38136136 Msa0640220:intron 50.0%
!! GACCCGAGGAGCATCACATT+AGG + chr4_3:38134201-38134220 None:intergenic 55.0%
!! ATGAAGGTGGTGGAAGGGGA+TGG - chr4_3:38134572-38134591 Msa0640220:CDS 55.0%
!! TGAAGGTGGTGGAAGGGGAT+GGG - chr4_3:38134573-38134592 Msa0640220:CDS 55.0%
TCATGGAGCCCCTGATACCA+TGG - chr4_3:38136341-38136360 Msa0640220:five_prime_UTR 55.0%
! TTGGGACATGATGGGACCCG+AGG + chr4_3:38134215-38134234 None:intergenic 60.0%
CGGGTCCCATCATGTCCCAA+TGG - chr4_3:38134215-38134234 Msa0640220:intron 60.0%
!! AGGGGATGGGACTAGCCCCA+AGG - chr4_3:38134586-38134605 Msa0640220:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 38132014 38136407 38132014 ID=Msa0640220;Name=Msa0640220
chr4_3 mRNA 38132014 38136407 38132014 ID=Msa0640220-mRNA-1;Parent=Msa0640220;Name=Msa0640220-mRNA-1;_AED=0.18;_eAED=0.18;_QI=80|0.5|0.11|1|0.5|0.11|9|0|504
chr4_3 exon 38132014 38132239 38132014 ID=Msa0640220-mRNA-1:exon:8665;Parent=Msa0640220-mRNA-1
chr4_3 exon 38132470 38132675 38132470 ID=Msa0640220-mRNA-1:exon:8664;Parent=Msa0640220-mRNA-1
chr4_3 exon 38133287 38133382 38133287 ID=Msa0640220-mRNA-1:exon:8663;Parent=Msa0640220-mRNA-1
chr4_3 exon 38133672 38133843 38133672 ID=Msa0640220-mRNA-1:exon:8662;Parent=Msa0640220-mRNA-1
chr4_3 exon 38134452 38134653 38134452 ID=Msa0640220-mRNA-1:exon:8661;Parent=Msa0640220-mRNA-1
chr4_3 exon 38134660 38134824 38134660 ID=Msa0640220-mRNA-1:exon:8660;Parent=Msa0640220-mRNA-1
chr4_3 exon 38135761 38135846 38135761 ID=Msa0640220-mRNA-1:exon:8659;Parent=Msa0640220-mRNA-1
chr4_3 exon 38135871 38136025 38135871 ID=Msa0640220-mRNA-1:exon:8658;Parent=Msa0640220-mRNA-1
chr4_3 exon 38136121 38136407 38136121 ID=Msa0640220-mRNA-1:exon:8657;Parent=Msa0640220-mRNA-1
chr4_3 five_prime_UTR 38136328 38136407 38136328 ID=Msa0640220-mRNA-1:five_prime_utr;Parent=Msa0640220-mRNA-1
chr4_3 CDS 38136121 38136327 38136121 ID=Msa0640220-mRNA-1:cds;Parent=Msa0640220-mRNA-1
chr4_3 CDS 38135871 38136025 38135871 ID=Msa0640220-mRNA-1:cds;Parent=Msa0640220-mRNA-1
chr4_3 CDS 38135761 38135846 38135761 ID=Msa0640220-mRNA-1:cds;Parent=Msa0640220-mRNA-1
chr4_3 CDS 38134660 38134824 38134660 ID=Msa0640220-mRNA-1:cds;Parent=Msa0640220-mRNA-1
chr4_3 CDS 38134452 38134653 38134452 ID=Msa0640220-mRNA-1:cds;Parent=Msa0640220-mRNA-1
chr4_3 CDS 38133672 38133843 38133672 ID=Msa0640220-mRNA-1:cds;Parent=Msa0640220-mRNA-1
chr4_3 CDS 38133287 38133382 38133287 ID=Msa0640220-mRNA-1:cds;Parent=Msa0640220-mRNA-1
chr4_3 CDS 38132470 38132675 38132470 ID=Msa0640220-mRNA-1:cds;Parent=Msa0640220-mRNA-1
chr4_3 CDS 38132014 38132239 38132014 ID=Msa0640220-mRNA-1:cds;Parent=Msa0640220-mRNA-1
Gene Sequence

>Msa0640220

ATGTTGTTGAACTCTTCCTTTAACTCACTTCCTAGTTTCTTCAAATCTCAAGAACTAGGAAGAACTAATAATCTCAGCCATAGAAATGGTTCTCCACTTCTATGTTACGCTACTAATACAAATGTTTCTCAAAAAAGAAAATCAGCCAATTACCAACCCAATATTTGGAATTACGATATCTTACAATCCTTGAAACACGATTATGCGGATGCTCGATACGTGGATAGGAGTAGGAGGATGCAAGAGGAAGTAAGAAGAATGATTAAGGATGAAAATGTAAAGATATTAGAGTTAATCGATACTGTGAAACACTTAGGTCTCAGTTATCAATTCGAAAAGGAGATAGGAGAAGCCTTTGATAGTGGTTCAAACAATATTGGCAGAAGTCTTCATGAAACTGCCTTAAGATTTAGGCTCCTTCGACAATATGGTTATGATGTTTCACCAGATATTTTTGAGATGTTTAAGGACCACAACGGAGATTTTAAGGCATGTCTTGTCCAAGATATCAAAGGAATGTTAAGTCTCTACGAGGCATCATTTCTATCTTATGAAGGAGAACAAATTTTGGACGAAGCCAATGCTTTCACTAGCATCCACCTCAAAGAACTTAGTAGAAGCAGTATCCTCTTTGATCAAGTGAATCATTCATTGGAGCTTCCACTACATCGTAGAGTCCAAAGTCTTGAAACTCGATGGTATATTGATTCATATGAAAAAAGGAAGGACGCAAACAAGGTCTTGCTCGAAGCAGCCAAATTGAATTTCAACATTGTGCAATCAACTCTTCAACAGGATCTTAAAGAAACGTCAAGGTGGTGGAAGGGGATGGGACTAGCCCCAAGGTTAAGCTTTGGTCGTGATAGGCTAATGGAATGTTTCTTTTGGGCTGCTGGAATGACACCATTTGAGCCTCAGTTTAGTGATATGCGCAAGGGTTTAACCAAAGTTTCCTCTCTAATTACCTTAATTGATGACATTTATGATATATTCTTCTTGGCACTTTACAACACTGTCAATGATTTCACATATGACACACTTAAAGAAACAGGACATGACATTCTTCCCTACCTTGTCAAAGCGTGGTCTGATTTGTTGAAAGCATTTCTACAAGAAGCAAGATGGTGTCACAACAAACACATGCCAAAATTTGATGACTACCTCAACAATGCATGGGTGTCTGTCTCCGGTGTAGTTTTACTCACCCATTCCTATTTTTTGCTAAATCGTAACATAACAAAGGAGGGGCTTGAGTACTTGGAAAATTGTCCTATATTGTTGCAGAGACCTGAGGAGGTTGCTTACAAACACATCCAAAAATTGCTTAATGAAACTTGGAAGAAGATGAACAAAGATCGAGTTATCAATTCACCTGCCTCAAAATATTTTTCAGAGACAATAATCAACCTTGCTAGAATTTCTCACTGCACGTACCAATATGGAGATGGTCATGGAGCCCCTGATACCATGGCAAAGAATCGAATAAAGGCATTAATACTTGAACCAATTAATTAA
Protein sequence

>Msa0640220

MLLNSSFNSLPSFFKSQELGRTNNLSHRNGSPLLCYATNTNVSQKRKSANYQPNIWNYDILQSLKHDYADARYVDRSRRMQEEVRRMIKDENVKILELIDTVKHLGLSYQFEKEIGEAFDSGSNNIGRSLHETALRFRLLRQYGYDVSPDIFEMFKDHNGDFKACLVQDIKGMLSLYEASFLSYEGEQILDEANAFTSIHLKELSRSSILFDQVNHSLELPLHRRVQSLETRWYIDSYEKRKDANKVLLEAAKLNFNIVQSTLQQDLKETSRWWKGMGLAPRLSFGRDRLMECFFWAAGMTPFEPQFSDMRKGLTKVSSLITLIDDIYDIFFLALYNTVNDFTYDTLKETGHDILPYLVKAWSDLLKAFLQEARWCHNKHMPKFDDYLNNAWVSVSGVVLLTHSYFLLNRNITKEGLEYLENCPILLQRPEEVAYKHIQKLLNETWKKMNKDRVINSPASKYFSETIINLARISHCTYQYGDGHGAPDTMAKNRIKALILEPIN*