AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0654300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0654300 MtrunA17_Chr4g0048921 92.133 572 43 2 1 572 1 570 0.0 1075
Msa0654300 MtrunA17_Chr4g0048911 48.824 510 247 8 66 569 55 556 4.92e-168 489
Msa0654300 MtrunA17_Chr3g0108991 44.422 493 264 6 82 567 23 512 5.74e-144 426
Msa0654300 MtrunA17_Chr3g0108951 44.422 493 264 6 82 567 23 512 5.74e-144 426
Msa0654300 MtrunA17_Chr2g0280581 44.646 495 261 9 82 567 27 517 9.34e-144 425
Msa0654300 MtrunA17_Chr3g0108971 44.286 490 263 6 85 567 1 487 1.03e-142 422
Msa0654300 MtrunA17_Chr3g0105691 44.422 493 264 6 82 567 56 545 1.12e-142 424
Msa0654300 MtrunA17_Chr3g0108941 43.522 494 268 6 82 567 23 513 4.18e-142 421
Msa0654300 MtrunA17_Chr3g0099031 44.135 503 265 9 75 567 16 512 9.22e-142 420
Msa0654300 MtrunA17_Chr3g0109031 43.408 493 269 6 82 567 23 512 1.16e-141 420
Msa0654300 MtrunA17_Chr6g0477111 43.878 490 265 6 85 567 1 487 2.37e-141 418
Msa0654300 MtrunA17_Chr3g0109021 43.611 493 268 6 82 567 23 512 2.81e-141 419
Msa0654300 MtrunA17_Chr3g0109011 43.673 490 266 6 85 567 1 487 3.54e-141 418
Msa0654300 MtrunA17_Chr3g0109001 43.320 494 269 6 82 567 23 513 1.32e-140 417
Msa0654300 MtrunA17_Chr3g0108981 43.177 491 268 6 85 567 1 488 1.25e-138 411
Msa0654300 MtrunA17_Chr3g0108961 43.177 491 268 6 85 567 1 488 1.25e-138 411
Msa0654300 MtrunA17_Chr6g0477121 45.606 421 222 5 143 559 5 422 2.16e-126 377
Msa0654300 MtrunA17_Chr2g0323131 36.965 514 299 7 65 568 62 560 3.74e-114 351
Msa0654300 MtrunA17_Chr2g0323151 38.174 482 289 4 87 568 3 475 1.02e-111 342
Msa0654300 MtrunA17_Chr4g0042111 35.608 469 294 4 90 555 79 542 3.76e-96 304
Msa0654300 MtrunA17_Chr2g0310331 34.961 512 320 7 66 571 80 584 2.82e-94 300
Msa0654300 MtrunA17_Chr3g0082691 33.012 518 334 5 63 571 38 551 2.10e-93 296
Msa0654300 MtrunA17_Chr4g0023911 34.369 483 310 4 78 557 85 563 3.35e-91 291
Msa0654300 MtrunA17_Chr7g0236991 32.866 499 325 4 63 555 39 533 1.27e-90 289
Msa0654300 MtrunA17_Chr7g0237001 32.665 499 326 4 63 555 39 533 5.57e-90 288
Msa0654300 MtrunA17_Chr7g0237041 32.665 499 326 4 63 555 39 533 5.57e-90 288
Msa0654300 MtrunA17_Chr7g0237081 32.665 499 326 4 63 555 39 533 5.57e-90 288
Msa0654300 MtrunA17_Chr7g0237051 32.665 499 326 4 63 555 39 533 5.57e-90 288
Msa0654300 MtrunA17_Chr7g0236961 33.403 476 311 3 82 555 7 478 5.75e-89 283
Msa0654300 MtrunA17_Chr6g0465441 31.660 518 340 8 63 571 39 551 1.81e-88 284
Msa0654300 MtrunA17_Chr7g0237011 33.193 476 312 3 82 555 7 478 5.66e-88 281
Msa0654300 MtrunA17_Chr5g0425371 32.885 520 331 8 63 571 39 551 6.09e-87 280
Msa0654300 MtrunA17_Chr5g0431651 31.650 515 338 5 67 571 40 550 1.30e-86 279
Msa0654300 MtrunA17_Chr3g0105071 31.599 519 342 5 63 572 42 556 1.70e-86 278
Msa0654300 MtrunA17_Chr4g0024691 31.311 511 339 5 69 571 53 559 2.39e-86 278
Msa0654300 MtrunA17_Chr6g0451711 31.214 519 342 6 63 571 42 555 4.21e-86 278
Msa0654300 MtrunA17_Chr5g0431571 31.907 514 337 5 67 571 38 547 1.51e-85 276
Msa0654300 MtrunA17_Chr8g0357411 33.266 496 316 9 82 571 101 587 4.91e-84 273
Msa0654300 MtrunA17_Chr2g0319051 32.422 512 326 9 71 571 10 512 4.57e-81 263
Msa0654300 MtrunA17_Chr8g0336181 32.000 525 328 11 65 571 41 554 4.15e-78 256
Msa0654300 MtrunA17_Chr6g0466051 29.207 517 354 5 63 571 39 551 4.19e-78 256
Msa0654300 MtrunA17_Chr6g0465851 29.014 517 355 5 63 571 4 516 1.20e-77 254
Msa0654300 MtrunA17_Chr6g0465911 29.014 517 355 5 63 571 39 551 3.12e-77 254
Msa0654300 MtrunA17_Chr2g0310401 34.689 418 266 5 145 560 5 417 5.77e-76 247
Msa0654300 MtrunA17_Chr2g0319041 29.905 525 344 11 63 571 39 555 7.23e-75 248
Msa0654300 MtrunA17_Chr7g0236971 32.987 385 254 2 171 555 1 381 7.96e-69 228
Msa0654300 MtrunA17_Chr4g0008621 34.109 387 231 8 63 433 47 425 1.73e-64 217
Msa0654300 MtrunA17_Chr7g0236951 37.542 301 185 2 82 380 7 306 1.28e-61 206
Msa0654300 MtrunA17_Chr7g0237031 37.209 301 186 2 82 380 7 306 6.40e-61 204
Msa0654300 MtrunA17_Chr6g0465931 34.700 317 200 3 63 373 4 319 2.42e-56 192
Msa0654300 MtrunA17_Chr6g0466111 34.385 317 201 3 63 373 4 319 1.35e-55 191
Msa0654300 MtrunA17_Chr6g0465981 34.385 317 201 3 63 373 39 354 4.70e-55 190
Msa0654300 MtrunA17_Chr6g0465871 34.385 317 201 3 63 373 39 354 4.70e-55 190
Msa0654300 MtrunA17_Chr6g0466081 34.385 317 201 3 63 373 39 354 4.70e-55 190
Msa0654300 MtrunA17_Chr8g0357231 35.878 262 160 5 313 571 1 257 2.14e-50 174
Msa0654300 MtrunA17_Chr7g0217361 33.688 282 182 3 268 548 6 283 1.52e-47 168
Msa0654300 MtrunA17_Chr2g0319071 27.642 492 298 14 88 571 46 487 2.93e-43 161
Msa0654300 MtrunA17_Chr5g0445511 37.156 218 128 4 167 380 2 214 1.42e-41 150
Msa0654300 MtrunA17_Chr3g0101681 25.144 521 338 13 88 572 277 781 5.07e-40 155
Msa0654300 MtrunA17_Chr2g0310121 25.359 418 284 8 88 481 276 689 4.06e-37 147
Msa0654300 MtrunA17_Chr2g0319081 28.485 330 212 9 246 571 116 425 4.44e-35 137
Msa0654300 MtrunA17_Chr7g0237061 28.395 243 147 2 313 555 2 217 1.54e-30 119
Msa0654300 MtrunA17_Chr6g0466031 24.904 261 190 3 313 571 2 258 1.97e-28 114
Msa0654300 MtrunA17_Chr7g0237071 35.204 196 125 1 82 275 7 202 2.22e-28 113
Msa0654300 MtrunA17_Chr2g0281651 27.200 250 173 5 250 497 45 287 7.48e-22 98.2
Msa0654300 MtrunA17_Chr8g0357221 32.812 192 126 2 82 272 101 290 7.32e-20 90.1
Msa0654300 MtrunA17_Chr7g0218001 27.376 263 159 8 88 320 347 607 1.18e-19 93.2
Msa0654300 MtrunA17_Chr6g0466021 35.971 139 83 2 63 195 39 177 1.90e-19 87.0
Msa0654300 MtrunA17_Chr5g0445501 27.746 173 120 2 401 571 1 170 1.67e-18 83.2
Msa0654300 MtrunA17_Chr7g0217351 30.476 210 134 6 70 273 70 273 1.78e-18 86.3
Msa0654300 MtrunA17_Chr7g0259881 25.185 270 172 8 88 328 230 498 1.87e-18 89.4
Msa0654300 MtrunA17_Chr7g0237021 27.742 155 109 1 401 555 1 152 1.04e-17 80.9
Msa0654300 MtrunA17_Chr7g0236941 27.742 155 109 1 401 555 1 152 1.04e-17 80.9
Msa0654300 MtrunA17_Chr7g0233861 28.986 138 89 1 208 345 30 158 2.82e-15 74.3
Msa0654300 MtrunA17_Chr6g0465881 22.543 173 129 2 401 571 1 170 1.27e-13 69.3
Msa0654300 MtrunA17_Chr6g0465941 22.543 173 129 2 401 571 1 170 1.59e-13 68.9
Msa0654300 MtrunA17_Chr6g0466121 22.543 173 129 2 401 571 1 170 1.59e-13 68.9
Msa0654300 MtrunA17_Chr6g0465991 22.543 173 129 2 401 571 1 170 1.59e-13 68.9
Msa0654300 MtrunA17_Chr6g0466091 22.543 173 129 2 401 571 1 170 1.59e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0654300 AT1G61680.1 45.709 571 289 10 6 567 11 569 2.05e-160 470
Msa0654300 AT1G61680.2 45.709 571 282 11 6 567 11 562 9.29e-158 463
Msa0654300 AT4G16730.1 33.588 524 322 8 67 571 21 537 6.75e-93 295
Msa0654300 AT4G16730.2 33.534 498 308 7 90 571 1 491 1.76e-90 287
Msa0654300 AT4G16740.1 33.786 515 298 13 65 557 52 545 9.80e-85 275
Msa0654300 AT3G25820.1 31.641 591 357 13 4 571 26 592 1.31e-84 275
Msa0654300 AT3G25830.1 31.641 591 357 13 4 571 26 592 1.31e-84 275
Msa0654300 AT2G24210.1 30.769 572 353 14 18 571 42 588 2.14e-81 266
Msa0654300 AT3G25810.1 31.041 509 324 10 82 571 95 595 3.46e-78 258
Msa0654300 AT5G23960.1 28.599 514 359 4 63 571 35 545 1.85e-71 239
Msa0654300 AT5G23960.2 28.571 511 357 4 65 571 37 543 6.64e-71 238
Msa0654300 AT1G70080.1 28.429 503 342 10 81 571 113 609 1.63e-63 219
Msa0654300 AT3G14520.1 26.369 493 350 7 72 557 100 586 3.87e-56 199
Msa0654300 AT3G25820.2 31.206 423 254 9 4 410 26 427 1.12e-54 191
Msa0654300 AT3G14490.1 26.984 504 352 7 64 557 85 582 4.11e-54 194
Msa0654300 AT3G14540.1 25.437 515 361 8 60 557 75 583 2.37e-53 191
Msa0654300 AT4G16740.2 34.195 348 197 10 65 390 52 389 3.91e-52 183
Msa0654300 AT3G29410.2 27.521 476 327 8 90 557 15 480 7.54e-52 185
Msa0654300 AT3G29410.1 27.521 476 327 8 90 557 120 585 6.28e-51 185
Msa0654300 AT1G31950.1 25.527 474 342 5 90 557 120 588 8.64e-51 184
Msa0654300 AT1G31950.3 25.527 474 342 5 90 557 121 589 8.86e-51 184
Msa0654300 AT4G13300.1 25.725 517 368 6 67 571 40 552 2.12e-48 177
Msa0654300 AT3G14540.2 25.754 431 303 5 60 475 75 503 3.00e-48 176
Msa0654300 AT4G20230.1 25.763 524 372 8 59 569 82 601 6.63e-48 177
Msa0654300 AT4G20230.2 25.763 524 372 8 59 569 94 613 7.57e-48 177
Msa0654300 AT5G44630.1 26.761 497 346 8 86 572 68 556 1.18e-46 172
Msa0654300 AT1G33750.1 24.213 508 372 6 69 568 96 598 1.49e-46 173
Msa0654300 AT4G15870.1 24.621 528 365 9 59 571 87 596 2.31e-46 172
Msa0654300 AT4G20200.1 26.061 495 358 4 82 571 111 602 6.52e-46 171
Msa0654300 AT4G13280.2 26.625 477 340 5 101 571 80 552 1.01e-45 169
Msa0654300 AT1G31950.2 25.558 403 294 3 90 487 121 522 6.12e-45 167
Msa0654300 AT3G29190.1 24.213 508 373 4 59 557 79 583 7.66e-44 165
Msa0654300 AT1G66020.1 26.236 526 370 8 59 571 76 596 9.35e-42 159
Msa0654300 AT4G16740.4 32.440 336 194 9 65 382 52 372 2.10e-41 154
Msa0654300 AT4G16740.3 32.440 336 194 9 65 382 52 372 2.10e-41 154
Msa0654300 AT1G48800.1 25.940 532 365 14 57 569 81 602 2.35e-41 158
Msa0654300 AT4G20210.1 24.860 535 379 10 42 567 74 594 2.30e-40 155
Msa0654300 AT5G48110.1 29.358 327 222 6 247 571 18 337 1.52e-38 145
Msa0654300 AT3G25820.4 30.447 358 213 8 4 345 26 363 2.60e-38 145
Msa0654300 AT3G25820.3 30.447 358 213 8 4 345 26 363 2.60e-38 145
Msa0654300 AT3G29110.1 24.762 525 379 8 59 571 75 595 5.41e-38 148
Msa0654300 AT3G32030.1 22.976 457 345 4 90 543 119 571 5.51e-38 148
Msa0654300 AT1G48820.1 23.909 527 348 11 53 569 77 560 7.14e-38 147
Msa0654300 AT2G23230.2 25.400 500 353 11 82 569 105 596 1.29e-37 147
Msa0654300 AT4G13280.1 25.577 477 315 7 101 571 80 522 1.88e-37 145
Msa0654300 AT2G23230.1 25.251 499 350 11 82 569 105 591 1.38e-36 144
Msa0654300 AT1G66020.2 27.010 311 218 3 59 360 76 386 1.03e-28 119
Msa0654300 AT1G79460.1 24.190 525 335 14 88 571 275 777 3.33e-26 114
Msa0654300 AT1G61120.1 25.263 380 246 8 128 485 333 696 3.82e-23 104
Msa0654300 AT4G02780.1 29.213 267 155 10 88 323 332 595 2.19e-17 86.7

Find 143 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCATGCTTCTTATATATTT+TGG 0.063401 4_3:+62487128 None:intergenic
CTTCGACTTTGGGATGATTT+AGG 0.178417 4_3:-62486745 Msa0654300:CDS
ATGACCACATGTACCATTTA+AGG 0.184226 4_3:+62487473 None:intergenic
GGTATTGGGATTAATAATTT+TGG 0.184662 4_3:+62485806 None:intergenic
ATTACATGAAAACATGTTTC+AGG 0.215235 4_3:-62487182 Msa0654300:CDS
CTGCCACAATTCTTCGACTT+TGG 0.232538 4_3:-62486756 Msa0654300:CDS
CAAAGTGGTAATCAATATTT+AGG 0.236989 4_3:+62488510 None:intergenic
AGACCTTTGTGAATGCTTTA+TGG 0.251154 4_3:+62485946 None:intergenic
TGTTCACTGAAGTTGTTTCT+AGG 0.259492 4_3:-62487330 Msa0654300:intron
TGCCACAATTCTTCGACTTT+GGG 0.273382 4_3:-62486755 Msa0654300:CDS
CATATTGGCTAATGGGTATT+GGG 0.284947 4_3:+62485792 None:intergenic
CCTTTCTAGTTGAAGCAAAA+TGG 0.291776 4_3:-62486942 Msa0654300:CDS
GATGATAAGCACTCCCTTCC+TGG 0.293520 4_3:-62485882 Msa0654300:CDS
TAGCGACATATTGTCTTCTT+AGG 0.299306 4_3:+62488468 None:intergenic
ATTATGTAGATTAAAGATAT+TGG 0.303530 4_3:+62487397 None:intergenic
TATTCATTCATATTGGCTAA+TGG 0.305020 4_3:+62485784 None:intergenic
GAAGCAAAATGGTTTGGTTC+AGG 0.309836 4_3:-62486931 Msa0654300:CDS
TCTTTCGAAGAGAGTCTTTA+GGG 0.310663 4_3:+62487100 None:intergenic
CTAGAAACCAGCCCCTTAAA+TGG 0.310744 4_3:-62487486 Msa0654300:CDS
ATTGAGCTATTCATTCATAT+TGG 0.325106 4_3:+62485777 None:intergenic
GTTAAATCCACTGATATGTT+TGG 0.335305 4_3:-62485674 Msa0654300:three_prime_UTR
TTGTGAATGCTTTATGGAAA+TGG 0.335415 4_3:+62485952 None:intergenic
ATTCATTCATATTGGCTAAT+GGG 0.337153 4_3:+62485785 None:intergenic
TGTGGCAGGGGCGGAAATAA+TGG 0.340825 4_3:+62486771 None:intergenic
ATATGAATGAATAGCTCAAT+AGG 0.355871 4_3:-62485774 Msa0654300:three_prime_UTR
TCATATTGGCTAATGGGTAT+TGG 0.362072 4_3:+62485791 None:intergenic
CTAGTTGAAGCAAAATGGTT+TGG 0.387597 4_3:-62486937 Msa0654300:CDS
GTCTTTCGAAGAGAGTCTTT+AGG 0.392280 4_3:+62487099 None:intergenic
AGGGGCTGGTTTCTAGCATA+TGG 0.394011 4_3:+62487493 None:intergenic
TATAGCACAAATGCATGGTT+TGG 0.394113 4_3:-62487891 Msa0654300:CDS
TATGAATGAATAGCTCAATA+GGG 0.399657 4_3:-62485773 Msa0654300:three_prime_UTR
TTCACAGCTAGAAATTTCTA+TGG 0.410766 4_3:-62487921 Msa0654300:CDS
TTCACGAAGCTAAGTTTGTA+AGG 0.412343 4_3:-62487983 Msa0654300:CDS
TGACCACATGTACCATTTAA+GGG 0.423595 4_3:+62487474 None:intergenic
TTAAATCCACTGATATGTTT+GGG 0.425924 4_3:-62485673 Msa0654300:three_prime_UTR
TTTGTGAAAAGGATACTCTA+AGG 0.428411 4_3:+62487956 None:intergenic
TAAATGGTACATGTGGTCAT+TGG 0.428916 4_3:-62487470 Msa0654300:CDS
GCTGAAGAATACTTAAAGAA+TGG 0.440968 4_3:-62486895 Msa0654300:CDS
TGTAGATGGTGGAGAGAACT+TGG 0.441935 4_3:-62487532 Msa0654300:intron
ATTTAGCAGGAAGATCCTTA+AGG 0.443162 4_3:-62488027 Msa0654300:CDS
TTTGGGTAAAGGATTAACTA+AGG 0.443181 4_3:-62486824 Msa0654300:CDS
TTTGGCACGCCTTGTGCTGA+AGG 0.448254 4_3:-62485592 Msa0654300:three_prime_UTR
CTGAAGAATACTTAAAGAAT+GGG 0.452903 4_3:-62486894 Msa0654300:CDS
ATGAGACCGCACTTCACTTC+AGG 0.453303 4_3:-62488412 Msa0654300:CDS
GCTGCAAGGCTTACACCATC+AGG 0.453407 4_3:-62487832 Msa0654300:CDS
ATGGGATATTGACACAGATA+TGG 0.458966 4_3:-62487217 Msa0654300:CDS
CAAGTATGTCTTCCCCTGCC+AGG 0.463054 4_3:+62488063 None:intergenic
ATTCTTTCCTCAGATAAAGT+AGG 0.467948 4_3:+62487433 None:intergenic
CTCTGGAGTGCATATTGTGC+TGG 0.472322 4_3:-62486863 Msa0654300:CDS
TGTGAATGCTTTATGGAAAT+GGG 0.472761 4_3:+62485953 None:intergenic
TGAGAAAGTTGTGCAATTGT+TGG 0.473300 4_3:+62488842 None:intergenic
CACTCCCTTCCTGGGCTAGA+GGG 0.473788 4_3:-62485873 Msa0654300:CDS
GAAAATCAACAAGGAAATGA+TGG 0.476240 4_3:-62486104 Msa0654300:CDS
GCCTCGCATCTAAACCTGGC+AGG 0.477164 4_3:-62488077 Msa0654300:CDS
GCAATTGTTGGGACGATTTG+AGG 0.478213 4_3:+62488854 None:intergenic
TCCAAAATATATAAGAAGCA+TGG 0.481912 4_3:-62487129 Msa0654300:CDS
TTACATGAAAACATGTTTCA+GGG 0.487840 4_3:-62487181 Msa0654300:CDS
CTCTTCACATTGCTAACAAG+TGG 0.489348 4_3:-62488793 Msa0654300:CDS
GAGAAAGTTGTGCAATTGTT+GGG 0.490482 4_3:+62488843 None:intergenic
AAATGGTACATGTGGTCATT+GGG 0.490912 4_3:-62487469 Msa0654300:CDS
GTTCATTTGTTAGATCATAG+AGG 0.495017 4_3:+62487157 None:intergenic
AAGCAAAATGGTTTGGTTCA+GGG 0.495976 4_3:-62486930 Msa0654300:CDS
CAAAGTCGAAGAATTGTGGC+AGG 0.496180 4_3:+62486757 None:intergenic
CTCACTCAATCTTGCAAGGA+TGG 0.499444 4_3:-62485925 Msa0654300:CDS
GTGTCTAATGATGGACCAAC+CGG 0.500032 4_3:-62486069 Msa0654300:CDS
AAACTAGGTGAAAATATCAA+TGG 0.502965 4_3:-62488119 Msa0654300:CDS
ATGAAGCTGGAAAATTTAGC+AGG 0.503149 4_3:-62488040 Msa0654300:CDS
ACATGTACCATTTAAGGGGC+TGG 0.504728 4_3:+62487479 None:intergenic
TTTAAGTATTCTTCAGCACT+TGG 0.505423 4_3:+62486901 None:intergenic
ATTCAATTTCTTCTTCAAAG+TGG 0.514024 4_3:+62488495 None:intergenic
AAGAATGGGATAGTAAGCTC+TGG 0.518549 4_3:-62486880 Msa0654300:CDS
GTTGGTGAAAATTCACTCAA+AGG 0.524144 4_3:-62488560 Msa0654300:CDS
CCAAAATATATAAGAAGCAT+GGG 0.524424 4_3:-62487128 Msa0654300:CDS
GCATTAATGTTGAAGAAGAA+AGG 0.527443 4_3:-62488769 Msa0654300:CDS
CCCTGCCAGGTTTAGATGCG+AGG 0.529393 4_3:+62488076 None:intergenic
ATCAATATTTAGGCGCTGCA+TGG 0.529573 4_3:+62488520 None:intergenic
GCAGGTTTGGGTATCAGTTG+TGG 0.531244 4_3:-62485699 Msa0654300:three_prime_UTR
TGAATAAAATATCTAATGCA+TGG 0.532850 4_3:-62486007 Msa0654300:CDS
TGAAGCCTCGCATCTAAACC+TGG 0.533102 4_3:-62488081 Msa0654300:CDS
CCACTGATATGTTTGGGTAT+CGG 0.533311 4_3:-62485667 Msa0654300:three_prime_UTR
ATGATAAGCACTCCCTTCCT+GGG 0.538683 4_3:-62485881 Msa0654300:CDS
GTATATTCATTCCTTTGCCA+TGG 0.540974 4_3:-62488912 Msa0654300:exon
CAAAGAGACACTCTTGGTTG+AGG 0.541785 4_3:+62485979 None:intergenic
TTGGCACGCCTTGTGCTGAA+GGG 0.544809 4_3:-62485591 Msa0654300:three_prime_UTR
ATCGATTTCAGTTTGCTGCA+AGG 0.551439 4_3:-62487846 Msa0654300:CDS
TCATCATAACTATACATCAA+TGG 0.553674 4_3:+62485900 None:intergenic
ATACGTGAAGTGTCTAATGA+TGG 0.556244 4_3:-62486078 Msa0654300:CDS
GCACTCCCTTCCTGGGCTAG+AGG 0.557886 4_3:-62485874 Msa0654300:CDS
GGTTTGGGTATCAGTTGTGG+TGG 0.559756 4_3:-62485696 Msa0654300:three_prime_UTR
CTTCTTGTACAATAATACTC+CGG 0.561255 4_3:+62486050 None:intergenic
CCTCGCATCTAAACCTGGCA+GGG 0.562204 4_3:-62488076 Msa0654300:CDS
GAAGACATACTTGATGAAGC+TGG 0.565437 4_3:-62488053 Msa0654300:CDS
ATCCCAAAGTCGAAGAATTG+TGG 0.568131 4_3:+62486753 None:intergenic
CTTTATCATAAAGCAAGGAA+TGG 0.568777 4_3:+62485844 None:intergenic
CAAGGTCACTTTGTTCCCGC+AGG 0.569400 4_3:-62488377 Msa0654300:intron
TTTGAGGTTTCAAAGAGGAA+AGG 0.569570 4_3:+62488870 None:intergenic
CTCGCATCTAAACCTGGCAG+GGG 0.571060 4_3:-62488075 Msa0654300:CDS
CAAATGCAGTGCCATGGCAA+AGG 0.571735 4_3:+62488901 None:intergenic
GAAAAGTTTGATCCAAAACT+AGG 0.575633 4_3:-62488134 Msa0654300:CDS
CAGGGGCGGAAATAATGGAT+AGG 0.578243 4_3:+62486776 None:intergenic
TGGACATAACCCTCTAGCCC+AGG 0.581943 4_3:+62485864 None:intergenic
AAATTTCTAGCTGTGAACAT+TGG 0.584942 4_3:+62487927 None:intergenic
ATTTATATAGCACAAATGCA+TGG 0.589084 4_3:-62487896 Msa0654300:CDS
TAAAGACTCTCTTCGAAAGA+CGG 0.589563 4_3:-62487097 Msa0654300:intron
AGGTCTCACTCAATCTTGCA+AGG 0.596435 4_3:-62485929 Msa0654300:CDS
AATATATAAGAAGCATGGGT+GGG 0.597265 4_3:-62487124 Msa0654300:CDS
GTGGTCATTGGGATGCCTCA+CGG 0.597557 4_3:-62487458 Msa0654300:CDS
TAAGGAAAATGTTCATACAA+TGG 0.597774 4_3:-62486806 Msa0654300:CDS
TCGAAGAATTGTGGCAGGGG+CGG 0.597807 4_3:+62486762 None:intergenic
AAGTCGAAGAATTGTGGCAG+GGG 0.600683 4_3:+62486759 None:intergenic
AAATATATAAGAAGCATGGG+TGG 0.602804 4_3:-62487125 Msa0654300:CDS
CATAACCCTCTAGCCCAGGA+AGG 0.603462 4_3:+62485868 None:intergenic
CACGGATCCTACTTTATCTG+AGG 0.604127 4_3:-62487440 Msa0654300:CDS
AGGGGCGGAAATAATGGATA+GGG 0.604700 4_3:+62486777 None:intergenic
GACCACATGTACCATTTAAG+GGG 0.604906 4_3:+62487475 None:intergenic
AATATACGTAAGTCAAGTAG+TGG 0.607241 4_3:+62488928 None:intergenic
TTGTGCTGAAGGGATTGCTG+TGG 0.608283 4_3:-62485581 Msa0654300:three_prime_UTR
ATGAATGAATAGCTCAATAG+GGG 0.608411 4_3:-62485772 Msa0654300:three_prime_UTR
GGGTATCAAAGAGACACTCT+TGG 0.612403 4_3:+62485973 None:intergenic
ACAGCAATCCCTTCAGCACA+AGG 0.613899 4_3:+62485583 None:intergenic
AAGCATGAACTTAGAAACGT+TGG 0.614659 4_3:-62488578 Msa0654300:CDS
CAATATGTCGCTAGTGCTTG+TGG 0.614746 4_3:-62488458 Msa0654300:CDS
AAATTTGAACAATCTCCTGA+TGG 0.624669 4_3:+62487817 None:intergenic
GTTTAACAAGTTCACTAACA+AGG 0.624782 4_3:-62488159 Msa0654300:CDS
ATAACCCTCTAGCCCAGGAA+GGG 0.629515 4_3:+62485869 None:intergenic
CCGATACCCAAACATATCAG+TGG 0.633009 4_3:+62485667 None:intergenic
AGCCCCTTAAATGGTACATG+TGG 0.636992 4_3:-62487477 Msa0654300:CDS
TAATCATTCTGAGAAACTTG+AGG 0.638838 4_3:-62488606 Msa0654300:CDS
TTGTACAATAATACTCCGGT+TGG 0.640064 4_3:+62486054 None:intergenic
AAAGTCGAAGAATTGTGGCA+GGG 0.644806 4_3:+62486758 None:intergenic
TGATTGCAAATGCAGTGCCA+TGG 0.662207 4_3:+62488895 None:intergenic
GACGATTTGAGGTTTCAAAG+AGG 0.663177 4_3:+62488865 None:intergenic
TCATTCTGAGAAACTTGAGG+TGG 0.664975 4_3:-62488603 Msa0654300:CDS
AACATACAATCAGTCCAATG+AGG 0.671430 4_3:+62485625 None:intergenic
GGATGATTTAGGAAACGCAG+AGG 0.673607 4_3:-62486734 Msa0654300:intron
TTTCCATAAAGCATTCACAA+AGG 0.678455 4_3:-62485949 Msa0654300:CDS
TTCAGGCTATTAAGACAACA+AGG 0.681647 4_3:-62488395 Msa0654300:CDS
AAGAAGAAGAGCAACAGACG+AGG 0.695455 4_3:-62486033 Msa0654300:CDS
TAATAGCCTGAAGTGAAGTG+CGG 0.695756 4_3:+62488406 None:intergenic
TAACAAGTTCACTAACAAGG+AGG 0.697685 4_3:-62488156 Msa0654300:CDS
TATGTCGCTAGTGCTTGTGG+TGG 0.710715 4_3:-62488455 Msa0654300:CDS
CAGATAAAGTAGGATCCGTG+AGG 0.735628 4_3:+62487443 None:intergenic
ATATAGGACGAAAATCAACA+AGG 0.742989 4_3:-62486113 Msa0654300:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAAAATTAAAAATTA+AGG + chr4_3:62486964-62486983 None:intergenic 0.0%
!!! TTTTAATTTTTAATTTATTT+TGG - chr4_3:62486968-62486987 Msa0654300:intron 0.0%
!! AAAATTAAAAATTAAGGAAT+AGG + chr4_3:62486958-62486977 None:intergenic 10.0%
!! AATTAATGATTAATAATTGT+TGG - chr4_3:62487277-62487296 Msa0654300:intron 10.0%
!! ATTAATGATTAATAATTGTT+GGG - chr4_3:62487278-62487297 Msa0654300:intron 10.0%
!!! ATAAAACTTCTTTTTATATA+TGG + chr4_3:62487969-62487988 None:intergenic 10.0%
!! TATTATTATTCTATAATATG+TGG - chr4_3:62488349-62488368 Msa0654300:intron 10.0%
!! GATAGTAGTAAATTAAAATT+TGG + chr4_3:62485810-62485829 None:intergenic 15.0%
!!! ATTTTAATTTACTACTATCT+TGG - chr4_3:62485811-62485830 Msa0654300:three_prime_UTR 15.0%
!! GAATAATTTATTGAGATTAA+AGG - chr4_3:62486757-62486776 Msa0654300:CDS 15.0%
!! ATTATGTAGATTAAAGATAT+TGG + chr4_3:62487129-62487148 None:intergenic 15.0%
!!! ATGACATTTTTGATATTTAT+GGG - chr4_3:62487151-62487170 Msa0654300:CDS 15.0%
!!! AATAGTTTAGAGTTTAATTT+AGG - chr4_3:62487826-62487845 Msa0654300:CDS 15.0%
!!! ATAGTTTAGAGTTTAATTTA+GGG - chr4_3:62487827-62487846 Msa0654300:CDS 15.0%
!! AAATGATTGTTATATACTTA+GGG + chr4_3:62487993-62488012 None:intergenic 15.0%
!! AAAATGATTGTTATATACTT+AGG + chr4_3:62487994-62488013 None:intergenic 15.0%
!!! TCATCTACTATATTTTTTAA+AGG + chr4_3:62488084-62488103 None:intergenic 15.0%
!!! TCTTTTTAATTTATATTGGG+AGG - chr4_3:62486307-62486326 Msa0654300:intron 20.0%
!!! ATGTATGCTAATTAATTTGA+TGG - chr4_3:62486735-62486754 Msa0654300:CDS 20.0%
!!! TTAAACGGGTTTTAAATAAA+AGG + chr4_3:62486862-62486881 None:intergenic 20.0%
!!! TTAATTTATTTTGGTGTAGA+TGG - chr4_3:62486977-62486996 Msa0654300:intron 20.0%
!!! GATGACATTTTTGATATTTA+TGG - chr4_3:62487150-62487169 Msa0654300:CDS 20.0%
!!! AAACATGTTTTCATGTAATT+TGG + chr4_3:62487340-62487359 None:intergenic 20.0%
!! TAAGCATAAGTAAGTAATAT+AGG - chr4_3:62487508-62487527 Msa0654300:CDS 20.0%
!!! ACTATTTTCATCATATGTTT+GGG - chr4_3:62488203-62488222 Msa0654300:intron 20.0%
!! ACAAGTCAATAAATCATAAT+TGG + chr4_3:62488287-62488306 None:intergenic 20.0%
!! TGAATAAAATATCTAATGCA+TGG - chr4_3:62488516-62488535 Msa0654300:CDS 20.0%
!! AAAAACTTTATCATAAAGCA+AGG + chr4_3:62488687-62488706 None:intergenic 20.0%
!! TTCTTCAAAAAAAACTCAAT+AGG - chr4_3:62488778-62488797 Msa0654300:CDS 20.0%
!! TCTTCAAAAAAAACTCAATA+GGG - chr4_3:62488779-62488798 Msa0654300:CDS 20.0%
!!! AATAATTTATTTTGCTAAGC+AGG - chr4_3:62488806-62488825 Msa0654300:CDS 20.0%
! CAAAGTGGTAATCAATATTT+AGG + chr4_3:62486016-62486035 None:intergenic 25.0%
! ATTCAATTTCTTCTTCAAAG+TGG + chr4_3:62486031-62486050 None:intergenic 25.0%
! AAAAAAATGAAGTGCATGTT+TGG + chr4_3:62486248-62486267 None:intergenic 25.0%
!! TTGATATTTTCACCTAGTTT+TGG + chr4_3:62486404-62486423 None:intergenic 25.0%
! AAACTAGGTGAAAATATCAA+TGG - chr4_3:62486404-62486423 Msa0654300:intron 25.0%
!! ATTGGCAAATTTTTGTGAAA+AGG + chr4_3:62486581-62486600 None:intergenic 25.0%
! ATTTATATAGCACAAATGCA+TGG - chr4_3:62486627-62486646 Msa0654300:intron 25.0%
!! AAGAGTTGTGATTGAATTTT+AGG - chr4_3:62486905-62486924 Msa0654300:CDS 25.0%
!! AGAGTTGTGATTGAATTTTA+GGG - chr4_3:62486906-62486925 Msa0654300:CDS 25.0%
! CTTGTTACATTACATTTCTT+AGG - chr4_3:62487229-62487248 Msa0654300:intron 25.0%
! ATTACATGAAAACATGTTTC+AGG - chr4_3:62487341-62487360 Msa0654300:CDS 25.0%
! TTACATGAAAACATGTTTCA+GGG - chr4_3:62487342-62487361 Msa0654300:CDS 25.0%
! TCCAAAATATATAAGAAGCA+TGG - chr4_3:62487394-62487413 Msa0654300:CDS 25.0%
!! CCAAAATATATAAGAAGCAT+GGG - chr4_3:62487395-62487414 Msa0654300:CDS 25.0%
! CTGAAGAATACTTAAAGAAT+GGG - chr4_3:62487629-62487648 Msa0654300:intron 25.0%
!! GTTCACATTTTCTTTCTTTT+GGG - chr4_3:62487682-62487701 Msa0654300:intron 25.0%
!!! ATTTTCTTTCTTTTGGGTAA+AGG - chr4_3:62487688-62487707 Msa0654300:intron 25.0%
! TAAGGAAAATGTTCATACAA+TGG - chr4_3:62487717-62487736 Msa0654300:intron 25.0%
! TTATATATGGACTTGTATGA+TGG + chr4_3:62487956-62487975 None:intergenic 25.0%
!!! TCTTGTGATTGATTTTCATT+GGG + chr4_3:62488118-62488137 None:intergenic 25.0%
!!! TTCTTGTGATTGATTTTCAT+TGG + chr4_3:62488119-62488138 None:intergenic 25.0%
!! GACTATTTTCATCATATGTT+TGG - chr4_3:62488202-62488221 Msa0654300:intron 25.0%
! GTTTGTGACAACATAAATAT+AGG - chr4_3:62488394-62488413 Msa0654300:CDS 25.0%
! TCATCATAACTATACATCAA+TGG + chr4_3:62488626-62488645 None:intergenic 25.0%
!!! GGTATTGGGATTAATAATTT+TGG + chr4_3:62488720-62488739 None:intergenic 25.0%
!! ATTCATTCATATTGGCTAAT+GGG + chr4_3:62488741-62488760 None:intergenic 25.0%
!! TATTCATTCATATTGGCTAA+TGG + chr4_3:62488742-62488761 None:intergenic 25.0%
! ATTGAGCTATTCATTCATAT+TGG + chr4_3:62488749-62488768 None:intergenic 25.0%
! ATATGAATGAATAGCTCAAT+AGG - chr4_3:62488749-62488768 Msa0654300:CDS 25.0%
! TATGAATGAATAGCTCAATA+GGG - chr4_3:62488750-62488769 Msa0654300:CDS 25.0%
! CTTCAAAAAAAACTCAATAG+GGG - chr4_3:62488780-62488799 Msa0654300:CDS 25.0%
! TTAAATCCACTGATATGTTT+GGG - chr4_3:62488850-62488869 Msa0654300:CDS 25.0%
!! TGTATGTTTAGTAGTCTTTT+TGG - chr4_3:62488913-62488932 Msa0654300:exon 25.0%
AATATACGTAAGTCAAGTAG+TGG + chr4_3:62485598-62485617 None:intergenic 30.0%
GCATTAATGTTGAAGAAGAA+AGG - chr4_3:62485754-62485773 Msa0654300:three_prime_UTR 30.0%
! TAATCATTCTGAGAAACTTG+AGG - chr4_3:62485917-62485936 Msa0654300:CDS 30.0%
AAATTGCTCTTAAGTTATGC+AGG + chr4_3:62486287-62486306 None:intergenic 30.0%
GTTTAACAAGTTCACTAACA+AGG - chr4_3:62486364-62486383 Msa0654300:intron 30.0%
GAAAAGTTTGATCCAAAACT+AGG - chr4_3:62486389-62486408 Msa0654300:intron 30.0%
GAAGATCCTTAAGGAAAAAA+TGG - chr4_3:62486505-62486524 Msa0654300:intron 30.0%
!!! ATTGTGCCATTTTTTCCTTA+AGG + chr4_3:62486514-62486533 None:intergenic 30.0%
TTTGTGAAAAGGATACTCTA+AGG + chr4_3:62486570-62486589 None:intergenic 30.0%
!! AAATTTCTAGCTGTGAACAT+TGG + chr4_3:62486599-62486618 None:intergenic 30.0%
TTCACAGCTAGAAATTTCTA+TGG - chr4_3:62486602-62486621 Msa0654300:intron 30.0%
AAATTTGAACAATCTCCTGA+TGG + chr4_3:62486709-62486728 None:intergenic 30.0%
GAAGAGTTGTGATTAATTGT+CGG + chr4_3:62486814-62486833 None:intergenic 30.0%
ACGTCATGTTAAAGTTAAAC+GGG + chr4_3:62486876-62486895 None:intergenic 30.0%
CACGTCATGTTAAAGTTAAA+CGG + chr4_3:62486877-62486896 None:intergenic 30.0%
!!! ATTTATTTTGGTGTAGATGG+TGG - chr4_3:62486980-62486999 Msa0654300:intron 30.0%
ATTCTTTCCTCAGATAAAGT+AGG + chr4_3:62487093-62487112 None:intergenic 30.0%
! GTTCATTTGTTAGATCATAG+AGG + chr4_3:62487369-62487388 None:intergenic 30.0%
! CCCATGCTTCTTATATATTT+TGG + chr4_3:62487398-62487417 None:intergenic 30.0%
! AAATATATAAGAAGCATGGG+TGG - chr4_3:62487398-62487417 Msa0654300:CDS 30.0%
! AATATATAAGAAGCATGGGT+GGG - chr4_3:62487399-62487418 Msa0654300:CDS 30.0%
! ATATTTTACCATTTGTGCAG+TGG - chr4_3:62487542-62487561 Msa0654300:intron 30.0%
! TATTTTACCATTTGTGCAGT+GGG - chr4_3:62487543-62487562 Msa0654300:intron 30.0%
TTTAAGTATTCTTCAGCACT+TGG + chr4_3:62487625-62487644 None:intergenic 30.0%
GCTGAAGAATACTTAAAGAA+TGG - chr4_3:62487628-62487647 Msa0654300:intron 30.0%
! GGTTCACATTTTCTTTCTTT+TGG - chr4_3:62487681-62487700 Msa0654300:intron 30.0%
TTTGGGTAAAGGATTAACTA+AGG - chr4_3:62487699-62487718 Msa0654300:intron 30.0%
ATATAGGACGAAAATCAACA+AGG - chr4_3:62488410-62488429 Msa0654300:CDS 30.0%
GAAAATCAACAAGGAAATGA+TGG - chr4_3:62488419-62488438 Msa0654300:CDS 30.0%
CTTCTTGTACAATAATACTC+CGG + chr4_3:62488476-62488495 None:intergenic 30.0%
TGTGAATGCTTTATGGAAAT+GGG + chr4_3:62488573-62488592 None:intergenic 30.0%
TTGTGAATGCTTTATGGAAA+TGG + chr4_3:62488574-62488593 None:intergenic 30.0%
! TTTCCATAAAGCATTCACAA+AGG - chr4_3:62488574-62488593 Msa0654300:CDS 30.0%
CTTTATCATAAAGCAAGGAA+TGG + chr4_3:62488682-62488701 None:intergenic 30.0%
ATGAATGAATAGCTCAATAG+GGG - chr4_3:62488751-62488770 Msa0654300:CDS 30.0%
!!! TTTATTTTGCTAAGCAGGTT+TGG - chr4_3:62488811-62488830 Msa0654300:CDS 30.0%
!! TTATTTTGCTAAGCAGGTTT+GGG - chr4_3:62488812-62488831 Msa0654300:CDS 30.0%
GTTAAATCCACTGATATGTT+TGG - chr4_3:62488849-62488868 Msa0654300:CDS 30.0%
GTATATTCATTCCTTTGCCA+TGG - chr4_3:62485611-62485630 Msa0654300:three_prime_UTR 35.0%
! TTTGAGGTTTCAAAGAGGAA+AGG + chr4_3:62485656-62485675 None:intergenic 35.0%
GAGAAAGTTGTGCAATTGTT+GGG + chr4_3:62485683-62485702 None:intergenic 35.0%
TGAGAAAGTTGTGCAATTGT+TGG + chr4_3:62485684-62485703 None:intergenic 35.0%
AAGCATGAACTTAGAAACGT+TGG - chr4_3:62485945-62485964 Msa0654300:CDS 35.0%
! GTTGGTGAAAATTCACTCAA+AGG - chr4_3:62485963-62485982 Msa0654300:CDS 35.0%
TAGCGACATATTGTCTTCTT+AGG + chr4_3:62486058-62486077 None:intergenic 35.0%
TTCAGGCTATTAAGACAACA+AGG - chr4_3:62486128-62486147 Msa0654300:intron 35.0%
TATGCAGGAAAAGGATCAAA+AGG + chr4_3:62486272-62486291 None:intergenic 35.0%
CTCTTAAGTTATGCAGGAAA+AGG + chr4_3:62486281-62486300 None:intergenic 35.0%
ATTCGAATTTACTATCGCAG+AGG - chr4_3:62486340-62486359 Msa0654300:intron 35.0%
TAACAAGTTCACTAACAAGG+AGG - chr4_3:62486367-62486386 Msa0654300:intron 35.0%
ATGAAGCTGGAAAATTTAGC+AGG - chr4_3:62486483-62486502 Msa0654300:intron 35.0%
ATTTAGCAGGAAGATCCTTA+AGG - chr4_3:62486496-62486515 Msa0654300:intron 35.0%
TTCACGAAGCTAAGTTTGTA+AGG - chr4_3:62486540-62486559 Msa0654300:intron 35.0%
TATAGCACAAATGCATGGTT+TGG - chr4_3:62486632-62486651 Msa0654300:intron 35.0%
! CGTTTAACTTTAACATGACG+TGG - chr4_3:62486875-62486894 Msa0654300:CDS 35.0%
! AATTAAGGAATAGGCGTTGT+CGG + chr4_3:62486949-62486968 None:intergenic 35.0%
TGACCACATGTACCATTTAA+GGG + chr4_3:62487052-62487071 None:intergenic 35.0%
ATGACCACATGTACCATTTA+AGG + chr4_3:62487053-62487072 None:intergenic 35.0%
TAAATGGTACATGTGGTCAT+TGG - chr4_3:62487053-62487072 Msa0654300:intron 35.0%
AAATGGTACATGTGGTCATT+GGG - chr4_3:62487054-62487073 Msa0654300:intron 35.0%
!! TGTTCACTGAAGTTGTTTCT+AGG - chr4_3:62487193-62487212 Msa0654300:CDS 35.0%
TAATAATTGTTGGGTGCAGA+TGG - chr4_3:62487287-62487306 Msa0654300:intron 35.0%
AATAATTGTTGGGTGCAGAT+GGG - chr4_3:62487288-62487307 Msa0654300:intron 35.0%
ATGGGATATTGACACAGATA+TGG - chr4_3:62487306-62487325 Msa0654300:intron 35.0%
TCTTTCGAAGAGAGTCTTTA+GGG + chr4_3:62487426-62487445 None:intergenic 35.0%
TAAAGACTCTCTTCGAAAGA+CGG - chr4_3:62487426-62487445 Msa0654300:CDS 35.0%
! CCATTTTGCTTCAACTAGAA+AGG + chr4_3:62487584-62487603 None:intergenic 35.0%
!! CCTTTCTAGTTGAAGCAAAA+TGG - chr4_3:62487581-62487600 Msa0654300:intron 35.0%
CTAGTTGAAGCAAAATGGTT+TGG - chr4_3:62487586-62487605 Msa0654300:intron 35.0%
!! AAGCAAAATGGTTTGGTTCA+GGG - chr4_3:62487593-62487612 Msa0654300:intron 35.0%
! ATACGTGAAGTGTCTAATGA+TGG - chr4_3:62488445-62488464 Msa0654300:CDS 35.0%
TTGTACAATAATACTCCGGT+TGG + chr4_3:62488472-62488491 None:intergenic 35.0%
AGACCTTTGTGAATGCTTTA+TGG + chr4_3:62488580-62488599 None:intergenic 35.0%
! CATATTGGCTAATGGGTATT+GGG + chr4_3:62488734-62488753 None:intergenic 35.0%
! TCATATTGGCTAATGGGTAT+TGG + chr4_3:62488735-62488754 None:intergenic 35.0%
!!! TCTGTTTTGTTGTTCCTCAT+TGG - chr4_3:62488884-62488903 Msa0654300:CDS 35.0%
AACATACAATCAGTCCAATG+AGG + chr4_3:62488901-62488920 None:intergenic 35.0%
! GACGATTTGAGGTTTCAAAG+AGG + chr4_3:62485661-62485680 None:intergenic 40.0%
CTCTTCACATTGCTAACAAG+TGG - chr4_3:62485730-62485749 Msa0654300:three_prime_UTR 40.0%
! TCATTCTGAGAAACTTGAGG+TGG - chr4_3:62485920-62485939 Msa0654300:CDS 40.0%
! ATCAATATTTAGGCGCTGCA+TGG + chr4_3:62486006-62486025 None:intergenic 40.0%
TAATAGCCTGAAGTGAAGTG+CGG + chr4_3:62486120-62486139 None:intergenic 40.0%
ACGCACTTAAAACTAACCTG+CGG + chr4_3:62486165-62486184 None:intergenic 40.0%
! GCATGTTTGGAATTACAGTG+AGG + chr4_3:62486235-62486254 None:intergenic 40.0%
GAAGACATACTTGATGAAGC+TGG - chr4_3:62486470-62486489 Msa0654300:intron 40.0%
!! ATCGATTTCAGTTTGCTGCA+AGG - chr4_3:62486677-62486696 Msa0654300:intron 40.0%
GACCACATGTACCATTTAAG+GGG + chr4_3:62487051-62487070 None:intergenic 40.0%
GTCTTTCGAAGAGAGTCTTT+AGG + chr4_3:62487427-62487446 None:intergenic 40.0%
!! GAAGCAAAATGGTTTGGTTC+AGG - chr4_3:62487592-62487611 Msa0654300:intron 40.0%
AAGAATGGGATAGTAAGCTC+TGG - chr4_3:62487643-62487662 Msa0654300:intron 40.0%
AAAGTCGAAGAATTGTGGCA+GGG + chr4_3:62487768-62487787 None:intergenic 40.0%
TGCCACAATTCTTCGACTTT+GGG - chr4_3:62487768-62487787 Msa0654300:intron 40.0%
ATCCCAAAGTCGAAGAATTG+TGG + chr4_3:62487773-62487792 None:intergenic 40.0%
!! CTTCGACTTTGGGATGATTT+AGG - chr4_3:62487778-62487797 Msa0654300:intron 40.0%
CCACTGATATGTTTGGGTAT+CGG - chr4_3:62488856-62488875 Msa0654300:CDS 40.0%
TGATTGCAAATGCAGTGCCA+TGG + chr4_3:62485631-62485650 None:intergenic 45.0%
GCAATTGTTGGGACGATTTG+AGG + chr4_3:62485672-62485691 None:intergenic 45.0%
CAATATGTCGCTAGTGCTTG+TGG - chr4_3:62486065-62486084 Msa0654300:CDS 45.0%
CGCACTTAAAACTAACCTGC+GGG + chr4_3:62486164-62486183 None:intergenic 45.0%
!! TGTAGATGGTGGAGAGAACT+TGG - chr4_3:62486991-62487010 Msa0654300:intron 45.0%
CTAGAAACCAGCCCCTTAAA+TGG - chr4_3:62487037-62487056 Msa0654300:intron 45.0%
ACATGTACCATTTAAGGGGC+TGG + chr4_3:62487047-62487066 None:intergenic 45.0%
AGCCCCTTAAATGGTACATG+TGG - chr4_3:62487046-62487065 Msa0654300:intron 45.0%
CAGATAAAGTAGGATCCGTG+AGG + chr4_3:62487083-62487102 None:intergenic 45.0%
CACGGATCCTACTTTATCTG+AGG - chr4_3:62487083-62487102 Msa0654300:intron 45.0%
AAACTCTCCCACTGCACAAA+TGG + chr4_3:62487553-62487572 None:intergenic 45.0%
!! AGGGGCGGAAATAATGGATA+GGG + chr4_3:62487749-62487768 None:intergenic 45.0%
AAGTCGAAGAATTGTGGCAG+GGG + chr4_3:62487767-62487786 None:intergenic 45.0%
CAAAGTCGAAGAATTGTGGC+AGG + chr4_3:62487769-62487788 None:intergenic 45.0%
CTGCCACAATTCTTCGACTT+TGG - chr4_3:62487767-62487786 Msa0654300:intron 45.0%
GGATGATTTAGGAAACGCAG+AGG - chr4_3:62487789-62487808 Msa0654300:intron 45.0%
!! GTGTCTAATGATGGACCAAC+CGG - chr4_3:62488454-62488473 Msa0654300:CDS 45.0%
AAGAAGAAGAGCAACAGACG+AGG - chr4_3:62488490-62488509 Msa0654300:CDS 45.0%
CAAAGAGACACTCTTGGTTG+AGG + chr4_3:62488547-62488566 None:intergenic 45.0%
GGGTATCAAAGAGACACTCT+TGG + chr4_3:62488553-62488572 None:intergenic 45.0%
AGGTCTCACTCAATCTTGCA+AGG - chr4_3:62488594-62488613 Msa0654300:CDS 45.0%
CTCACTCAATCTTGCAAGGA+TGG - chr4_3:62488598-62488617 Msa0654300:CDS 45.0%
ATGATAAGCACTCCCTTCCT+GGG - chr4_3:62488642-62488661 Msa0654300:intron 45.0%
CCGATACCCAAACATATCAG+TGG + chr4_3:62488859-62488878 None:intergenic 45.0%
!!! ATTTTCTTTTTAATTTATAT+TGG - chr4_3:62486303-62486322 Msa0654300:intron 5.0%
!!! TTTTCTTTTTAATTTATATT+GGG - chr4_3:62486304-62486323 Msa0654300:intron 5.0%
CAAATGCAGTGCCATGGCAA+AGG + chr4_3:62485625-62485644 None:intergenic 50.0%
TATGTCGCTAGTGCTTGTGG+TGG - chr4_3:62486068-62486087 Msa0654300:CDS 50.0%
ATGAGACCGCACTTCACTTC+AGG - chr4_3:62486111-62486130 Msa0654300:intron 50.0%
TGAAGCCTCGCATCTAAACC+TGG - chr4_3:62486442-62486461 Msa0654300:intron 50.0%
!! AGGGGCTGGTTTCTAGCATA+TGG + chr4_3:62487033-62487052 None:intergenic 50.0%
!! CTCTGGAGTGCATATTGTGC+TGG - chr4_3:62487660-62487679 Msa0654300:intron 50.0%
!! CAGGGGCGGAAATAATGGAT+AGG + chr4_3:62487750-62487769 None:intergenic 50.0%
GATGATAAGCACTCCCTTCC+TGG - chr4_3:62488641-62488660 Msa0654300:intron 50.0%
ATAACCCTCTAGCCCAGGAA+GGG + chr4_3:62488657-62488676 None:intergenic 50.0%
GCAGGTTTGGGTATCAGTTG+TGG - chr4_3:62488824-62488843 Msa0654300:CDS 50.0%
GGTTTGGGTATCAGTTGTGG+TGG - chr4_3:62488827-62488846 Msa0654300:CDS 50.0%
ACAGCAATCCCTTCAGCACA+AGG + chr4_3:62488943-62488962 None:intergenic 50.0%
TTGTGCTGAAGGGATTGCTG+TGG - chr4_3:62488942-62488961 Msa0654300:five_prime_UTR 50.0%
! CAAGGTCACTTTGTTCCCGC+AGG - chr4_3:62486146-62486165 Msa0654300:intron 55.0%
CCTCGCATCTAAACCTGGCA+GGG - chr4_3:62486447-62486466 Msa0654300:intron 55.0%
CTCGCATCTAAACCTGGCAG+GGG - chr4_3:62486448-62486467 Msa0654300:intron 55.0%
CAAGTATGTCTTCCCCTGCC+AGG + chr4_3:62486463-62486482 None:intergenic 55.0%
GCTGCAAGGCTTACACCATC+AGG - chr4_3:62486691-62486710 Msa0654300:intron 55.0%
GTGGTCATTGGGATGCCTCA+CGG - chr4_3:62487065-62487084 Msa0654300:intron 55.0%
TGTGGCAGGGGCGGAAATAA+TGG + chr4_3:62487755-62487774 None:intergenic 55.0%
TCGAAGAATTGTGGCAGGGG+CGG + chr4_3:62487764-62487783 None:intergenic 55.0%
CATAACCCTCTAGCCCAGGA+AGG + chr4_3:62488658-62488677 None:intergenic 55.0%
TGGACATAACCCTCTAGCCC+AGG + chr4_3:62488662-62488681 None:intergenic 55.0%
TTTGGCACGCCTTGTGCTGA+AGG - chr4_3:62488931-62488950 Msa0654300:five_prime_UTR 55.0%
TTGGCACGCCTTGTGCTGAA+GGG - chr4_3:62488932-62488951 Msa0654300:five_prime_UTR 55.0%
GCCTCGCATCTAAACCTGGC+AGG - chr4_3:62486446-62486465 Msa0654300:intron 60.0%
CCCTGCCAGGTTTAGATGCG+AGG + chr4_3:62486450-62486469 None:intergenic 60.0%
! CACTCCCTTCCTGGGCTAGA+GGG - chr4_3:62488650-62488669 Msa0654300:intron 60.0%
! GCACTCCCTTCCTGGGCTAG+AGG - chr4_3:62488649-62488668 Msa0654300:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 62485557 62488988 62485557 ID=Msa0654300;Name=Msa0654300
chr4_3 mRNA 62485557 62488988 62485557 ID=Msa0654300-mRNA-1;Parent=Msa0654300;Name=Msa0654300-mRNA-1;_AED=0.06;_eAED=0.06;_QI=73|1|1|1|1|1|7|279|572
chr4_3 exon 62485557 62486129 62485557 ID=Msa0654300-mRNA-1:exon:17336;Parent=Msa0654300-mRNA-1
chr4_3 exon 62486735 62486983 62486735 ID=Msa0654300-mRNA-1:exon:17335;Parent=Msa0654300-mRNA-1
chr4_3 exon 62487098 62487239 62487098 ID=Msa0654300-mRNA-1:exon:17334;Parent=Msa0654300-mRNA-1
chr4_3 exon 62487331 62487549 62487331 ID=Msa0654300-mRNA-1:exon:17333;Parent=Msa0654300-mRNA-1
chr4_3 exon 62487813 62488185 62487813 ID=Msa0654300-mRNA-1:exon:17332;Parent=Msa0654300-mRNA-1
chr4_3 exon 62488378 62488648 62488378 ID=Msa0654300-mRNA-1:exon:17331;Parent=Msa0654300-mRNA-1
chr4_3 exon 62488745 62488988 62488745 ID=Msa0654300-mRNA-1:exon:17330;Parent=Msa0654300-mRNA-1
chr4_3 five_prime_UTR 62488916 62488988 62488916 ID=Msa0654300-mRNA-1:five_prime_utr;Parent=Msa0654300-mRNA-1
chr4_3 CDS 62488745 62488915 62488745 ID=Msa0654300-mRNA-1:cds;Parent=Msa0654300-mRNA-1
chr4_3 CDS 62488378 62488648 62488378 ID=Msa0654300-mRNA-1:cds;Parent=Msa0654300-mRNA-1
chr4_3 CDS 62487813 62488185 62487813 ID=Msa0654300-mRNA-1:cds;Parent=Msa0654300-mRNA-1
chr4_3 CDS 62487331 62487549 62487331 ID=Msa0654300-mRNA-1:cds;Parent=Msa0654300-mRNA-1
chr4_3 CDS 62487098 62487239 62487098 ID=Msa0654300-mRNA-1:cds;Parent=Msa0654300-mRNA-1
chr4_3 CDS 62486735 62486983 62486735 ID=Msa0654300-mRNA-1:cds;Parent=Msa0654300-mRNA-1
chr4_3 CDS 62485836 62486129 62485836 ID=Msa0654300-mRNA-1:cds;Parent=Msa0654300-mRNA-1
chr4_3 three_prime_UTR 62485557 62485835 62485557 ID=Msa0654300-mRNA-1:three_prime_utr;Parent=Msa0654300-mRNA-1
Gene Sequence

>Msa0654300

ATGGCACTGCATTTGCAATCATGCCTTTCCTCTTTGAAACCTCAAATCGTCCCAACAATTGCACAACTTTCTCAAAACTTTAATAATCTCAAGCTTGATTCTCTTCACATTGCTAACAAGTGGAGCATTAATGTTGAAGAAGAAAGGCGTAGTGCACTCATAAAACAACATAGAGATCTCAGCAGCAATAATAATCATTCTGAGAAACTTGAGGTGGTTAAGCATGAACTTAGAAACGTTGGTGAAAATTCACTCAAAGGTTTGTATATGATTGATGCCATGCAGCGCCTAAATATTGATTACCACTTTGAAGAAGAAATTGAATCATTCCTAAGAAGACAATATGTCGCTAGTGCTTGTGGTGGAAGTAATCATCATAATCTTCATGAGACCGCACTTCACTTCAGGCTATTAAGACAACAAGGTCACTTTGTTCCCGCAGAGGTGTTTAACAAGTTCACTAACAAGGAGGAAAAGTTTGATCCAAAACTAGGTGAAAATATCAATGGAATGATAGATTTATTTGAAGCCTCGCATCTAAACCTGGCAGGGGAAGACATACTTGATGAAGCTGGAAAATTTAGCAGGAAGATCCTTAAGGAAAAAATGGCACAATTTGATTTTCACGAAGCTAAGTTTGTAAGGAGAACCTTAGAGTATCCTTTTCACAAAAATTTGCCAATGTTCACAGCTAGAAATTTCTATGGTCATTTATATAGCACAAATGCATGGTTTGGTTCTATGAAAGAAGTTGCGAAAATCGATTTCAGTTTGCTGCAAGGCTTACACCATCAGGAGATTGTTCAAATTTCAAAATGGTGGAGAGAACTTGGTTTAGCTAATGAATTACCATATGCTAGAAACCAGCCCCTTAAATGGTACATGTGGTCATTGGGATGCCTCACGGATCCTACTTTATCTGAGGAAAGAATTGAGCTCACAAAACCAATATCTTTAATCTACATAATTGATGACATTTTTGATATTTATGGGACTCTAGATGAGTTAACTCTGTTCACTGAAGTTGTTTCTAGATGGGATATTGACACAGATATGGAGCAACTACCAAATTACATGAAAACATGTTTCAGGGTCCTCTATGATCTAACAAATGAACTAAGCTCCAAAATATATAAGAAGCATGGGTGGGACCCTAAAGACTCTCTTCGAAAGACGTGGGAGAGTTTGTGCAAAGCCTTTCTAGTTGAAGCAAAATGGTTTGGTTCAGGGAAGTTGCCAAGTGCTGAAGAATACTTAAAGAATGGGATAGTAAGCTCTGGAGTGCATATTGTGCTGGTTCACATTTTCTTTCTTTTGGGTAAAGGATTAACTAAGGAAAATGTTCATACAATGGACACAACCCTATCCATTATTTCCGCCCCTGCCACAATTCTTCGACTTTGGGATGATTTAGGAAACGCAGAGGACGAAAATCAACAAGGAAATGATGGATCATACGTGAAGTGTCTAATGATGGACCAACCGGAGTATTATTGTACAAGAAGAAGAGCAACAGACGAGGTTATGAATAAAATATCTAATGCATGGAAAAGCCTCAACCAAGAGTGTCTCTTTGATACCCATTTCCATAAAGCATTCACAAAGGTCTCACTCAATCTTGCAAGGATGGTACCATTGATGTATAGTTATGATGATAAGCACTCCCTTCCTGGGCTAGAGGGTTATGTCCATTCCTTGCTTTATGATAAAGTTTTTTGA
Protein sequence

>Msa0654300

MALHLQSCLSSLKPQIVPTIAQLSQNFNNLKLDSLHIANKWSINVEEERRSALIKQHRDLSSNNNHSEKLEVVKHELRNVGENSLKGLYMIDAMQRLNIDYHFEEEIESFLRRQYVASACGGSNHHNLHETALHFRLLRQQGHFVPAEVFNKFTNKEEKFDPKLGENINGMIDLFEASHLNLAGEDILDEAGKFSRKILKEKMAQFDFHEAKFVRRTLEYPFHKNLPMFTARNFYGHLYSTNAWFGSMKEVAKIDFSLLQGLHHQEIVQISKWWRELGLANELPYARNQPLKWYMWSLGCLTDPTLSEERIELTKPISLIYIIDDIFDIYGTLDELTLFTEVVSRWDIDTDMEQLPNYMKTCFRVLYDLTNELSSKIYKKHGWDPKDSLRKTWESLCKAFLVEAKWFGSGKLPSAEEYLKNGIVSSGVHIVLVHIFFLLGKGLTKENVHTMDTTLSIISAPATILRLWDDLGNAEDENQQGNDGSYVKCLMMDQPEYYCTRRRATDEVMNKISNAWKSLNQECLFDTHFHKAFTKVSLNLARMVPLMYSYDDKHSLPGLEGYVHSLLYDKVF*