AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0699960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 95 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGGCCTTGCTGGTTATAAT+AGG 0.198318 4_4:+70772593 Msa0699960:three_prime_UTR
ATCATTCTTCAAGAATTATA+AGG 0.232662 4_4:+70771594 Msa0699960:three_prime_UTR
AATCTTTCTTTGTCTAAATC+TGG 0.297789 4_4:+70771944 Msa0699960:three_prime_UTR
CAAAGGGTTTAAGAGATAGA+AGG 0.305323 4_4:+70771666 Msa0699960:three_prime_UTR
ATAAGGGTTTCAAGAGCTTC+TGG 0.308884 4_4:+70771611 Msa0699960:three_prime_UTR
TGATCTGGTATGAATGAAAA+TGG 0.312077 4_4:-70772487 None:intergenic
GCACATCATCATCATCTATT+AGG 0.320825 4_4:+70772349 Msa0699960:three_prime_UTR
ACAATCTCTTCGGGCCTTGC+TGG 0.321697 4_4:+70772583 Msa0699960:three_prime_UTR
CTGAATTTCACAATCTCTTC+GGG 0.336767 4_4:+70772574 Msa0699960:three_prime_UTR
AACTCTTGGGAATTTATGTT+GGG 0.338800 4_4:-70771474 None:intergenic
CAACTCTTGGGAATTTATGT+TGG 0.340066 4_4:-70771475 None:intergenic
TACTTTAGTCCAATGTTTCC+AGG 0.350694 4_4:+70772310 Msa0699960:three_prime_UTR
ACGCACCTGCTAGAAGAAAA+TGG 0.361537 4_4:-70772906 None:intergenic
TTGGCTGTGTAGTGATAAAA+AGG 0.361791 4_4:-70771218 None:intergenic
ATCATTAGAAGAGGTGGAGG+AGG 0.362000 4_4:+70771527 Msa0699960:three_prime_UTR
CTTGGCTGATTATTATTACT+AGG 0.375750 4_4:-70771848 None:intergenic
ATGAAAGCACAAATCTTAAC+AGG 0.378339 4_4:+70771555 Msa0699960:three_prime_UTR
GGTTCTTCAGCTGACCCTCT+TGG 0.378386 4_4:-70772220 None:intergenic
GGGCCTTGCTGGTTATAATA+GGG 0.382791 4_4:+70772594 Msa0699960:three_prime_UTR
TGAAAATTCAGAGACTGTTT+TGG 0.385223 4_4:-70771237 None:intergenic
AACCATTCAACAGCTTTACT+TGG 0.387306 4_4:-70771755 None:intergenic
AGACAAAAGAGACGGTGAAT+TGG 0.387420 4_4:-70772629 None:intergenic
TCATTCTTCAAGAATTATAA+GGG 0.401881 4_4:+70771595 Msa0699960:three_prime_UTR
ATCTTAACAGGTTCAATAGT+TGG 0.402776 4_4:+70771567 Msa0699960:three_prime_UTR
TCTGAATTTCACAATCTCTT+CGG 0.406508 4_4:+70772573 Msa0699960:three_prime_UTR
AGAGTGTTTGTGTGTGGTGT+GGG 0.415765 4_4:-70771295 None:intergenic
CTCAACAACTCATTATTCTC+TGG 0.418248 4_4:+70771818 Msa0699960:three_prime_UTR
TGACCCTCTTGGACTATTTG+TGG 0.423757 4_4:-70772209 None:intergenic
TCTGAGACAAGTAAAGGTTC+TGG 0.423833 4_4:+70771980 Msa0699960:three_prime_UTR
AAAACTGATGGTGTTGAATC+TGG 0.424465 4_4:-70772801 None:intergenic
GAGATCATTAGAAGAGGTGG+AGG 0.430301 4_4:+70771524 Msa0699960:three_prime_UTR
GAGCTTCTGGTGGAAAAGAT+AGG 0.444208 4_4:+70771624 Msa0699960:three_prime_UTR
AGAGAGATCATTAGAAGAGG+TGG 0.446541 4_4:+70771521 Msa0699960:three_prime_UTR
GATATATCACGGTCCAAGTC+AGG 0.450823 4_4:-70771179 None:intergenic
GCTCTTGATGAAGAAGATCC+TGG 0.452930 4_4:-70772328 None:intergenic
CTCTTTCTCTAGCTTTGACA+CGG 0.453585 4_4:-70772036 None:intergenic
TGTGTGGTGTGGGGTTAGCT+AGG 0.457010 4_4:-70771285 None:intergenic
TTCGGTTCATGATGCTGAAA+TGG 0.457200 4_4:+70771886 Msa0699960:three_prime_UTR
TGATGATTATTCTCCATTGT+AGG 0.461933 4_4:-70772766 None:intergenic
GAATGAAGCTGTTTGAGAAA+TGG 0.464042 4_4:-70772146 None:intergenic
GTTTAAGAGATAGAAGGGTT+AGG 0.467094 4_4:+70771672 Msa0699960:three_prime_UTR
TAAAGAAGGGCCTCCTGACT+TGG 0.481244 4_4:+70771166 Msa0699960:CDS
AGTAAAGTAATGACTTCAAA+GGG 0.486371 4_4:+70771650 Msa0699960:three_prime_UTR
TTCACTGAACTACTTACTTG+TGG 0.489069 4_4:+70772166 Msa0699960:three_prime_UTR
AAGTGAACAAGGTGTTGATT+CGG 0.495908 4_4:+70771868 Msa0699960:three_prime_UTR
GAAGAAGATCCTGGAAACAT+TGG 0.496215 4_4:-70772319 None:intergenic
ATTCAACAGCTTTACTTGGT+TGG 0.503152 4_4:-70771751 None:intergenic
ATGGAATTCTTCAGCACCAA+TGG 0.510387 4_4:+70772282 Msa0699960:three_prime_UTR
GCTGAAGGTGAAGCTGAAGC+AGG 0.512953 4_4:-70772739 None:intergenic
GAGAGTGTTTGTGTGTGGTG+TGG 0.514090 4_4:-70771296 None:intergenic
GAATCAACACCTTGTTCACT+TGG 0.514307 4_4:-70771866 None:intergenic
ACCTTCAGCTGCTCCTACAA+TGG 0.515548 4_4:+70772753 Msa0699960:three_prime_UTR
CAGCTCTATTCAGATCAGAA+AGG 0.516369 4_4:+70772850 Msa0699960:three_prime_UTR
TAATCATTGTTGTTCCCGCT+TGG 0.517589 4_4:-70772547 None:intergenic
AGCTCTATTCAGATCAGAAA+GGG 0.521442 4_4:+70772851 Msa0699960:three_prime_UTR
AAAGGGTTTAAGAGATAGAA+GGG 0.523321 4_4:+70771667 Msa0699960:three_prime_UTR
GGACTAAAGTAATGATCCAT+TGG 0.525062 4_4:-70772298 None:intergenic
AATAATGAGTTGTTGAGTGA+AGG 0.525467 4_4:-70771812 None:intergenic
GAAAATGAAGAAGAAAATCA+TGG 0.536388 4_4:+70771497 Msa0699960:three_prime_UTR
GCTGAAATGGTTGTTGAGAA+TGG 0.538563 4_4:+70771899 Msa0699960:three_prime_UTR
TAGTAAAGTAATGACTTCAA+AGG 0.544163 4_4:+70771649 Msa0699960:three_prime_UTR
TTTCATCATCATCAATGCAA+AGG 0.546814 4_4:+70771255 Msa0699960:CDS
TTCTCACCACTAGTACTGAA+TGG 0.555766 4_4:-70772421 None:intergenic
AACCAAGTAAAGCTGTTGAA+TGG 0.558567 4_4:+70771753 Msa0699960:three_prime_UTR
GCAAAGGCATGATTGTGGTA+TGG 0.565396 4_4:+70771433 Msa0699960:three_prime_UTR
TCAAAGCTAGAGAAAGAGCA+AGG 0.568563 4_4:+70772041 Msa0699960:three_prime_UTR
GGCCTTGCTGGTTATAATAG+GGG 0.569734 4_4:+70772595 Msa0699960:three_prime_UTR
AAAGTGAAGTTAGACCAAGT+TGG 0.576073 4_4:+70771401 Msa0699960:three_prime_UTR
GAGTGTTTGTGTGTGGTGTG+GGG 0.577546 4_4:-70771294 None:intergenic
AATAATCAGCCAAGTGAACA+AGG 0.578757 4_4:+70771857 Msa0699960:three_prime_UTR
GTTTCACCATTCAGTACTAG+TGG 0.581208 4_4:+70772415 Msa0699960:three_prime_UTR
AGATTGTTGGATCCATCTAA+AGG 0.583522 4_4:-70772676 None:intergenic
AGCACTTCTGAGACAAGTAA+AGG 0.584944 4_4:+70771974 Msa0699960:three_prime_UTR
CTTCTTCTACTCAACCAAGC+GGG 0.586398 4_4:+70772533 Msa0699960:three_prime_UTR
TATGATCTTCAAGATAGACT+TGG 0.589871 4_4:+70771722 Msa0699960:three_prime_UTR
GGAAAGAACTGCTAAAGAGA+AGG 0.598537 4_4:+70772063 Msa0699960:three_prime_UTR
GAGACGGTGAATTGGACTGA+AGG 0.599533 4_4:-70772621 None:intergenic
GACAACAGCAGCAGCAACAA+TGG 0.601712 4_4:+70772263 Msa0699960:three_prime_UTR
GCATGATTGTGGTATGGAGG+AGG 0.610814 4_4:+70771439 Msa0699960:three_prime_UTR
AGATCATATGATGCAAAATG+TGG 0.615631 4_4:+70772504 Msa0699960:three_prime_UTR
CAAAGCTAGAGAAAGAGCAA+GGG 0.619608 4_4:+70772042 Msa0699960:three_prime_UTR
GGAAGAGAGATCATTAGAAG+AGG 0.619686 4_4:+70771518 Msa0699960:three_prime_UTR
AGGGTTTCAAGAGCTTCTGG+TGG 0.620264 4_4:+70771614 Msa0699960:three_prime_UTR
AGACGGTGAATTGGACTGAA+GGG 0.624249 4_4:-70772620 None:intergenic
GCCTTGCTGGTTATAATAGG+GGG 0.632630 4_4:+70772596 Msa0699960:three_prime_UTR
AGAGAGAGAGTGTTTGTGTG+TGG 0.640589 4_4:-70771301 None:intergenic
TCCATTGTAGGAGCAGCTGA+AGG 0.640995 4_4:-70772754 None:intergenic
TGAAGATGAGACAAAAGAGA+CGG 0.658703 4_4:-70772637 None:intergenic
GATGGTGTTGAATCTGGTGA+TGG 0.659814 4_4:-70772795 None:intergenic
ATATCACGGTCCAAGTCAGG+AGG 0.664320 4_4:-70771176 None:intergenic
ACTACCACAAATAGTCCAAG+AGG 0.670044 4_4:+70772205 Msa0699960:three_prime_UTR
TCTTCTTCTACTCAACCAAG+CGG 0.675315 4_4:+70772532 Msa0699960:three_prime_UTR
AAGGCATGATTGTGGTATGG+AGG 0.704505 4_4:+70771436 Msa0699960:three_prime_UTR
CTACCACAAATAGTCCAAGA+GGG 0.751118 4_4:+70772206 Msa0699960:three_prime_UTR
CACCTGCTAGAAGAAAATGG+AGG 0.774618 4_4:-70772903 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAATAATAATAACAA+TGG + chr4_4:70770352-70770371 Msa0699960:intron 10.0%
!! ATGATTAAAAAAATGTTGAA+GGG - chr4_4:70770570-70770589 None:intergenic 15.0%
!! AATGATTAAAAAAATGTTGA+AGG - chr4_4:70770571-70770590 None:intergenic 15.0%
!!! TAGCTTTTCATAAAATATAT+AGG + chr4_4:70770838-70770857 Msa0699960:intron 15.0%
!!! TAAAATATATAGGTTTTTTG+AGG + chr4_4:70770848-70770867 Msa0699960:intron 15.0%
!!! TCTTGTGAATTTTATATTGA+TGG + chr4_4:70770293-70770312 Msa0699960:intron 20.0%
!!! CTTGTGAATTTTATATTGAT+GGG + chr4_4:70770294-70770313 Msa0699960:intron 20.0%
!!! GATTTTGTATTTTGTTTCAA+TGG + chr4_4:70770390-70770409 Msa0699960:intron 20.0%
!! GTAGTACATATATATATGTA+GGG + chr4_4:70770802-70770821 Msa0699960:intron 20.0%
!! TGATAGAAAGTAATTATTTG+AGG + chr4_4:70771041-70771060 Msa0699960:intron 20.0%
!! ATATATCAGAGAGTAAAAAA+AGG + chr4_4:70771195-70771214 Msa0699960:CDS 20.0%
!! ATCATTCTTCAAGAATTATA+AGG + chr4_4:70771594-70771613 Msa0699960:three_prime_UTR 20.0%
!! TCATTCTTCAAGAATTATAA+GGG + chr4_4:70771595-70771614 Msa0699960:three_prime_UTR 20.0%
!!! TGATCTTATTTTGCATTTCA+AGG + chr4_4:70770132-70770151 Msa0699960:intron 25.0%
! ATCAAAGAAAAAGATGAAAG+TGG - chr4_4:70770255-70770274 None:intergenic 25.0%
! TCATTACTAAGAACAATGTT+TGG - chr4_4:70770543-70770562 None:intergenic 25.0%
! TTAAAAAAATGTTGAAGGGT+AGG - chr4_4:70770566-70770585 None:intergenic 25.0%
! CATATATATATGTACTACCA+TGG - chr4_4:70770800-70770819 None:intergenic 25.0%
! GGTAGTACATATATATATGT+AGG + chr4_4:70770801-70770820 Msa0699960:intron 25.0%
!! AGAAAGTAATTATTTGAGGT+TGG + chr4_4:70771045-70771064 Msa0699960:intron 25.0%
!! TTTTACTCTCTGATATATCA+CGG - chr4_4:70771193-70771212 None:intergenic 25.0%
! GAAAATGAAGAAGAAAATCA+TGG + chr4_4:70771497-70771516 Msa0699960:three_prime_UTR 25.0%
! TAGTAAAGTAATGACTTCAA+AGG + chr4_4:70771649-70771668 Msa0699960:three_prime_UTR 25.0%
! AGTAAAGTAATGACTTCAAA+GGG + chr4_4:70771650-70771669 Msa0699960:three_prime_UTR 25.0%
! AATCTTTCTTTGTCTAAATC+TGG + chr4_4:70771944-70771963 Msa0699960:three_prime_UTR 25.0%
!!! AACAATCTACCTTTTTTCAT+GGG + chr4_4:70772691-70772710 Msa0699960:three_prime_UTR 25.0%
AAAATCAGCAGCAAAGAATA+AGG + chr4_4:70770196-70770215 Msa0699960:intron 30.0%
!!! ATATTGATGGGTTCTTTTGT+TGG + chr4_4:70770306-70770325 Msa0699960:intron 30.0%
TACTAAGAACAATGTTTGGT+CGG - chr4_4:70770539-70770558 None:intergenic 30.0%
ACTATAATACAGCTACAACA+AGG - chr4_4:70770645-70770664 None:intergenic 30.0%
ATACTAGCAGAAGCTATAAA+AGG + chr4_4:70770698-70770717 Msa0699960:intron 30.0%
TACTAGCAGAAGCTATAAAA+GGG + chr4_4:70770699-70770718 Msa0699960:intron 30.0%
AAGCAGCAAAATTTGCATAA+GGG - chr4_4:70770743-70770762 None:intergenic 30.0%
AAAGCAGCAAAATTTGCATA+AGG - chr4_4:70770744-70770763 None:intergenic 30.0%
AGCTTAAAAACAAAGCTGAA+TGG + chr4_4:70770767-70770786 Msa0699960:intron 30.0%
!! AAGAGGTTTTGCTTTTGAAA+GGG + chr4_4:70771117-70771136 Msa0699960:CDS 30.0%
AAACTGCAAAAAGCTAAAGA+AGG + chr4_4:70771152-70771171 Msa0699960:CDS 30.0%
AACTGCAAAAAGCTAAAGAA+GGG + chr4_4:70771153-70771172 Msa0699960:CDS 30.0%
! TGAAAATTCAGAGACTGTTT+TGG - chr4_4:70771240-70771259 None:intergenic 30.0%
TTTCATCATCATCAATGCAA+AGG + chr4_4:70771255-70771274 Msa0699960:CDS 30.0%
TTTGCAAAAACAAACCAACT+TGG - chr4_4:70771418-70771437 None:intergenic 30.0%
!!! AAGTTGGTTTGTTTTTGCAA+AGG + chr4_4:70771417-70771436 Msa0699960:three_prime_UTR 30.0%
AACTCTTGGGAATTTATGTT+GGG - chr4_4:70771477-70771496 None:intergenic 30.0%
! CTTCTTCATTTTCAACTCTT+GGG - chr4_4:70771490-70771509 None:intergenic 30.0%
! TCTTCTTCATTTTCAACTCT+TGG - chr4_4:70771491-70771510 None:intergenic 30.0%
! ATGAAAGCACAAATCTTAAC+AGG + chr4_4:70771555-70771574 Msa0699960:three_prime_UTR 30.0%
ATCTTAACAGGTTCAATAGT+TGG + chr4_4:70771567-70771586 Msa0699960:three_prime_UTR 30.0%
AAAGGGTTTAAGAGATAGAA+GGG + chr4_4:70771667-70771686 Msa0699960:three_prime_UTR 30.0%
TATGATCTTCAAGATAGACT+TGG + chr4_4:70771722-70771741 Msa0699960:three_prime_UTR 30.0%
AATAATGAGTTGTTGAGTGA+AGG - chr4_4:70771815-70771834 None:intergenic 30.0%
CTTGGCTGATTATTATTACT+AGG - chr4_4:70771851-70771870 None:intergenic 30.0%
!! CTAGCTTTGTTAAAAAAGCT+AGG - chr4_4:70772244-70772263 None:intergenic 30.0%
TGATCTGGTATGAATGAAAA+TGG - chr4_4:70772490-70772509 None:intergenic 30.0%
! ACATTTTGCATCATATGATC+TGG - chr4_4:70772505-70772524 None:intergenic 30.0%
AGATCATATGATGCAAAATG+TGG + chr4_4:70772504-70772523 Msa0699960:three_prime_UTR 30.0%
TCTGAATTTCACAATCTCTT+CGG + chr4_4:70772573-70772592 Msa0699960:three_prime_UTR 30.0%
! TTTTCAGTTTCACCTTTAGA+TGG + chr4_4:70772664-70772683 Msa0699960:three_prime_UTR 30.0%
CATGAAAAAAGGTAGATTGT+TGG - chr4_4:70772692-70772711 None:intergenic 30.0%
!!! CAACAATCTACCTTTTTTCA+TGG + chr4_4:70772690-70772709 Msa0699960:three_prime_UTR 30.0%
TGATGATTATTCTCCATTGT+AGG - chr4_4:70772769-70772788 None:intergenic 30.0%
CAAAAACAGATGAAAACTGA+TGG - chr4_4:70772816-70772835 None:intergenic 30.0%
!!! CAGTTTTCATCTGTTTTTGA+TGG + chr4_4:70772817-70772836 Msa0699960:three_prime_UTR 30.0%
! AGTGAAACTCTTCAGTTTTT+GGG - chr4_4:70772880-70772899 None:intergenic 30.0%
! AAGTGAAACTCTTCAGTTTT+TGG - chr4_4:70772881-70772900 None:intergenic 30.0%
! TGTCTTTCCTTGGTGTAAAA+TGG - chr4_4:70770160-70770179 None:intergenic 35.0%
TTGCTGATATTGTCTTTCCT+TGG - chr4_4:70770170-70770189 None:intergenic 35.0%
! TGTGAGCTGTTTGAGTTTAA+GGG + chr4_4:70770432-70770451 Msa0699960:intron 35.0%
TTGAGTAATCAACAGCAACT+GGG + chr4_4:70770467-70770486 Msa0699960:intron 35.0%
! TAAAAGGGTTTGGCTTAGAT+CGG + chr4_4:70770714-70770733 Msa0699960:intron 35.0%
!! TTGGTTTGCTTAAGCCTTAA+TGG + chr4_4:70771064-70771083 Msa0699960:intron 35.0%
!! GAAGAGGTTTTGCTTTTGAA+AGG + chr4_4:70771116-70771135 Msa0699960:CDS 35.0%
TTGGCTGTGTAGTGATAAAA+AGG - chr4_4:70771221-70771240 None:intergenic 35.0%
AAAGTGAAGTTAGACCAAGT+TGG + chr4_4:70771401-70771420 Msa0699960:three_prime_UTR 35.0%
!! TTTTTGCAAAGGCATGATTG+TGG + chr4_4:70771428-70771447 Msa0699960:three_prime_UTR 35.0%
CAACTCTTGGGAATTTATGT+TGG - chr4_4:70771478-70771497 None:intergenic 35.0%
CAAAGGGTTTAAGAGATAGA+AGG + chr4_4:70771666-70771685 Msa0699960:three_prime_UTR 35.0%
GTTTAAGAGATAGAAGGGTT+AGG + chr4_4:70771672-70771691 Msa0699960:three_prime_UTR 35.0%
ATTCAACAGCTTTACTTGGT+TGG - chr4_4:70771754-70771773 None:intergenic 35.0%
AACCAAGTAAAGCTGTTGAA+TGG + chr4_4:70771753-70771772 Msa0699960:three_prime_UTR 35.0%
AACCATTCAACAGCTTTACT+TGG - chr4_4:70771758-70771777 None:intergenic 35.0%
CTCAACAACTCATTATTCTC+TGG + chr4_4:70771818-70771837 Msa0699960:three_prime_UTR 35.0%
AATAATCAGCCAAGTGAACA+AGG + chr4_4:70771857-70771876 Msa0699960:three_prime_UTR 35.0%
!! AAGTGAACAAGGTGTTGATT+CGG + chr4_4:70771868-70771887 Msa0699960:three_prime_UTR 35.0%
GAATGAAGCTGTTTGAGAAA+TGG - chr4_4:70772149-70772168 None:intergenic 35.0%
TTCACTGAACTACTTACTTG+TGG + chr4_4:70772166-70772185 Msa0699960:three_prime_UTR 35.0%
GGACTAAAGTAATGATCCAT+TGG - chr4_4:70772301-70772320 None:intergenic 35.0%
TACTTTAGTCCAATGTTTCC+AGG + chr4_4:70772310-70772329 Msa0699960:three_prime_UTR 35.0%
GCACATCATCATCATCTATT+AGG + chr4_4:70772349-70772368 Msa0699960:three_prime_UTR 35.0%
CTGAATTTCACAATCTCTTC+GGG + chr4_4:70772574-70772593 Msa0699960:three_prime_UTR 35.0%
TGAAGATGAGACAAAAGAGA+CGG - chr4_4:70772640-70772659 None:intergenic 35.0%
! CTCTTTTGTCTCATCTTCAA+AGG + chr4_4:70772641-70772660 Msa0699960:three_prime_UTR 35.0%
AGATTGTTGGATCCATCTAA+AGG - chr4_4:70772679-70772698 None:intergenic 35.0%
!!! AATCTACCTTTTTTCATGGG+AGG + chr4_4:70772694-70772713 Msa0699960:three_prime_UTR 35.0%
! AAAACTGATGGTGTTGAATC+TGG - chr4_4:70772804-70772823 None:intergenic 35.0%
AGCTCTATTCAGATCAGAAA+GGG + chr4_4:70772851-70772870 Msa0699960:three_prime_UTR 35.0%
!! CAAGGTACCATTTTACACCA+AGG + chr4_4:70770150-70770169 Msa0699960:intron 40.0%
!!! GGTTCTTTTGTTGGCTTAGT+GGG + chr4_4:70770315-70770334 Msa0699960:intron 40.0%
!!! GTTCTTTTGTTGGCTTAGTG+GGG + chr4_4:70770316-70770335 Msa0699960:intron 40.0%
! CTGTGAGCTGTTTGAGTTTA+AGG + chr4_4:70770431-70770450 Msa0699960:intron 40.0%
GTTGAGTAATCAACAGCAAC+TGG + chr4_4:70770466-70770485 Msa0699960:intron 40.0%
GCAGAAGCTATAAAAGGGTT+TGG + chr4_4:70770704-70770723 Msa0699960:intron 40.0%
TTGCTTAAGCCTTAATGGTG+TGG + chr4_4:70771069-70771088 Msa0699960:intron 40.0%
TTAAGCCTTAATGGTGTGGT+TGG + chr4_4:70771073-70771092 Msa0699960:intron 40.0%
CAAAACCAACCACACCATTA+AGG - chr4_4:70771081-70771100 None:intergenic 40.0%
!!! TGGTTGGTTTTGAGTTTTGC+AGG + chr4_4:70771089-70771108 Msa0699960:intron 40.0%
GGAAGAGAGATCATTAGAAG+AGG + chr4_4:70771518-70771537 Msa0699960:three_prime_UTR 40.0%
AGAGAGATCATTAGAAGAGG+TGG + chr4_4:70771521-70771540 Msa0699960:three_prime_UTR 40.0%
ATAAGGGTTTCAAGAGCTTC+TGG + chr4_4:70771611-70771630 Msa0699960:three_prime_UTR 40.0%
GAATCAACACCTTGTTCACT+TGG - chr4_4:70771869-70771888 None:intergenic 40.0%
TTCGGTTCATGATGCTGAAA+TGG + chr4_4:70771886-70771905 Msa0699960:three_prime_UTR 40.0%
GCTGAAATGGTTGTTGAGAA+TGG + chr4_4:70771899-70771918 Msa0699960:three_prime_UTR 40.0%
!! AGCACTTCTGAGACAAGTAA+AGG + chr4_4:70771974-70771993 Msa0699960:three_prime_UTR 40.0%
! TCTGAGACAAGTAAAGGTTC+TGG + chr4_4:70771980-70771999 Msa0699960:three_prime_UTR 40.0%
!! CTCTTTCTCTAGCTTTGACA+CGG - chr4_4:70772039-70772058 None:intergenic 40.0%
TCAAAGCTAGAGAAAGAGCA+AGG + chr4_4:70772041-70772060 Msa0699960:three_prime_UTR 40.0%
CAAAGCTAGAGAAAGAGCAA+GGG + chr4_4:70772042-70772061 Msa0699960:three_prime_UTR 40.0%
GGAAAGAACTGCTAAAGAGA+AGG + chr4_4:70772063-70772082 Msa0699960:three_prime_UTR 40.0%
ACTACCACAAATAGTCCAAG+AGG + chr4_4:70772205-70772224 Msa0699960:three_prime_UTR 40.0%
CTACCACAAATAGTCCAAGA+GGG + chr4_4:70772206-70772225 Msa0699960:three_prime_UTR 40.0%
ATGGAATTCTTCAGCACCAA+TGG + chr4_4:70772282-70772301 Msa0699960:three_prime_UTR 40.0%
GAAGAAGATCCTGGAAACAT+TGG - chr4_4:70772322-70772341 None:intergenic 40.0%
GTTTCACCATTCAGTACTAG+TGG + chr4_4:70772415-70772434 Msa0699960:three_prime_UTR 40.0%
! TTCTCACCACTAGTACTGAA+TGG - chr4_4:70772424-70772443 None:intergenic 40.0%
TCTTCTTCTACTCAACCAAG+CGG + chr4_4:70772532-70772551 Msa0699960:three_prime_UTR 40.0%
! TAATCATTGTTGTTCCCGCT+TGG - chr4_4:70772550-70772569 None:intergenic 40.0%
AGACAAAAGAGACGGTGAAT+TGG - chr4_4:70772632-70772651 None:intergenic 40.0%
CAGCTCTATTCAGATCAGAA+AGG + chr4_4:70772850-70772869 Msa0699960:three_prime_UTR 40.0%
! TTCCTCCATTTTCTTCTAGC+AGG + chr4_4:70772901-70772920 Msa0699960:three_prime_UTR 40.0%
!!! GGGTTCTTTTGTTGGCTTAG+TGG + chr4_4:70770314-70770333 Msa0699960:intron 45.0%
!! TTTGGTCGGAGTTTTGTGAC+AGG - chr4_4:70770525-70770544 None:intergenic 45.0%
AGCTGAATGGTTCAGATCCA+TGG + chr4_4:70770780-70770799 Msa0699960:intron 45.0%
! GAGTTTTGCAGGAAGAGAAG+AGG + chr4_4:70771100-70771119 Msa0699960:intron 45.0%
GATATATCACGGTCCAAGTC+AGG - chr4_4:70771182-70771201 None:intergenic 45.0%
! AGAGTGTTTGTGTGTGGTGT+GGG - chr4_4:70771298-70771317 None:intergenic 45.0%
!! AGAGAGAGAGTGTTTGTGTG+TGG - chr4_4:70771304-70771323 None:intergenic 45.0%
! GCAAAGGCATGATTGTGGTA+TGG + chr4_4:70771433-70771452 Msa0699960:three_prime_UTR 45.0%
AAGGCATGATTGTGGTATGG+AGG + chr4_4:70771436-70771455 Msa0699960:three_prime_UTR 45.0%
GAGATCATTAGAAGAGGTGG+AGG + chr4_4:70771524-70771543 Msa0699960:three_prime_UTR 45.0%
ATCATTAGAAGAGGTGGAGG+AGG + chr4_4:70771527-70771546 Msa0699960:three_prime_UTR 45.0%
GAGCTTCTGGTGGAAAAGAT+AGG + chr4_4:70771624-70771643 Msa0699960:three_prime_UTR 45.0%
TGACCCTCTTGGACTATTTG+TGG - chr4_4:70772212-70772231 None:intergenic 45.0%
GCTCTTGATGAAGAAGATCC+TGG - chr4_4:70772331-70772350 None:intergenic 45.0%
CTTCTTCTACTCAACCAAGC+GGG + chr4_4:70772533-70772552 Msa0699960:three_prime_UTR 45.0%
! GGGCCTTGCTGGTTATAATA+GGG + chr4_4:70772594-70772613 Msa0699960:three_prime_UTR 45.0%
! GGCCTTGCTGGTTATAATAG+GGG + chr4_4:70772595-70772614 Msa0699960:three_prime_UTR 45.0%
! GCCTTGCTGGTTATAATAGG+GGG + chr4_4:70772596-70772615 Msa0699960:three_prime_UTR 45.0%
TCCCCCTATTATAACCAGCA+AGG - chr4_4:70772600-70772619 None:intergenic 45.0%
AGACGGTGAATTGGACTGAA+GGG - chr4_4:70772623-70772642 None:intergenic 45.0%
!!! CTACCTTTTTTCATGGGAGG+AGG + chr4_4:70772697-70772716 Msa0699960:three_prime_UTR 45.0%
! GATGGTGTTGAATCTGGTGA+TGG - chr4_4:70772798-70772817 None:intergenic 45.0%
CACCTGCTAGAAGAAAATGG+AGG - chr4_4:70772906-70772925 None:intergenic 45.0%
ACGCACCTGCTAGAAGAAAA+TGG - chr4_4:70772909-70772928 None:intergenic 45.0%
TAAAGAAGGGCCTCCTGACT+TGG + chr4_4:70771166-70771185 Msa0699960:CDS 50.0%
ATATCACGGTCCAAGTCAGG+AGG - chr4_4:70771179-70771198 None:intergenic 50.0%
GAGTGTTTGTGTGTGGTGTG+GGG - chr4_4:70771297-70771316 None:intergenic 50.0%
!! GAGAGTGTTTGTGTGTGGTG+TGG - chr4_4:70771299-70771318 None:intergenic 50.0%
GCATGATTGTGGTATGGAGG+AGG + chr4_4:70771439-70771458 Msa0699960:three_prime_UTR 50.0%
AGGGTTTCAAGAGCTTCTGG+TGG + chr4_4:70771614-70771633 Msa0699960:three_prime_UTR 50.0%
GACAACAGCAGCAGCAACAA+TGG + chr4_4:70772263-70772282 Msa0699960:three_prime_UTR 50.0%
! CGGGCCTTGCTGGTTATAAT+AGG + chr4_4:70772593-70772612 Msa0699960:three_prime_UTR 50.0%
GAGACGGTGAATTGGACTGA+AGG - chr4_4:70772624-70772643 None:intergenic 50.0%
GCACCTCCTCCCATGAAAAA+AGG - chr4_4:70772703-70772722 None:intergenic 50.0%
ACCTTCAGCTGCTCCTACAA+TGG + chr4_4:70772753-70772772 Msa0699960:three_prime_UTR 50.0%
TCCATTGTAGGAGCAGCTGA+AGG - chr4_4:70772757-70772776 None:intergenic 50.0%
!! TGTGTGGTGTGGGGTTAGCT+AGG - chr4_4:70771288-70771307 None:intergenic 55.0%
GGTTCTTCAGCTGACCCTCT+TGG - chr4_4:70772223-70772242 None:intergenic 55.0%
ACAATCTCTTCGGGCCTTGC+TGG + chr4_4:70772583-70772602 Msa0699960:three_prime_UTR 55.0%
GCTGAAGGTGAAGCTGAAGC+AGG - chr4_4:70772742-70772761 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 70770053 70772947 70770053 ID=Msa0699960;Name=Msa0699960
chr4_4 mRNA 70770053 70772947 70770053 ID=Msa0699960-mRNA-1;Parent=Msa0699960;Name=Msa0699960-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0.5|1|0|0|2|1658|61
chr4_4 exon 70770053 70770059 70770053 ID=Msa0699960-mRNA-1:exon:21437;Parent=Msa0699960-mRNA-1
chr4_4 exon 70771111 70772947 70771111 ID=Msa0699960-mRNA-1:exon:21438;Parent=Msa0699960-mRNA-1
chr4_4 CDS 70770053 70770059 70770053 ID=Msa0699960-mRNA-1:cds;Parent=Msa0699960-mRNA-1
chr4_4 CDS 70771111 70771289 70771111 ID=Msa0699960-mRNA-1:cds;Parent=Msa0699960-mRNA-1
chr4_4 three_prime_UTR 70771290 70772947 70771290 ID=Msa0699960-mRNA-1:three_prime_utr;Parent=Msa0699960-mRNA-1
Gene Sequence

>Msa0699960

ATGTTAAGAAGAGAAGAGGTTTTGCTTTTGAAAGGGTTTTGTTCTGCAAAACTGCAAAAAGCTAAAGAAGGGCCTCCTGACTTGGACCGTGATATATCAGAGAGTAAAAAAAGGCCTTTTTATCACTACACAGCCAAAACAGTCTCTGAATTTTCATCATCATCAATGCAAAGGACATATACCTAG
Protein sequence

>Msa0699960

MLRREEVLLLKGFCSAKLQKAKEGPPDLDRDISESKKRPFYHYTAKTVSEFSSSSMQRTYT*