AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0700740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0700740 MtrunA17_Chr8g0378461 95.361 194 7 1 1 192 1 194 1.14e-131 367
Msa0700740 MtrunA17_Chr3g0121381 51.765 170 80 1 23 192 18 185 4.25e-57 178
Msa0700740 MtrunA17_Chr8g0356191 38.728 173 104 2 15 185 13 185 4.41e-39 132
Msa0700740 MtrunA17_Chr7g0219391 45.631 103 52 1 15 113 10 112 4.40e-27 99.8
Msa0700740 MtrunA17_Chr1g0205191 40.789 76 42 1 33 108 25 97 2.76e-12 60.8
Msa0700740 MtrunA17_Chr1g0205181 37.736 106 58 3 9 113 6 104 3.97e-12 61.2
Msa0700740 MtrunA17_Chr5g0395001 27.778 144 94 4 15 154 12 149 3.17e-11 59.3
Msa0700740 MtrunA17_Chr5g0394991 34.862 109 64 4 10 113 6 112 4.72e-11 59.7
Msa0700740 MtrunA17_Chr1g0205201 39.024 82 48 2 33 113 25 105 6.20e-11 58.2
Msa0700740 MtrunA17_Chr5g0429901 32.653 147 79 6 14 149 6 143 9.30e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0700740 AT2G44300.1 36.275 204 110 3 6 192 3 203 4.62e-42 140
Msa0700740 AT2G44290.1 35.567 194 107 2 14 192 14 204 1.19e-41 139
Msa0700740 AT1G55260.1 41.622 185 96 5 10 190 50 226 1.40e-41 140
Msa0700740 AT1G55260.2 41.209 182 98 4 10 190 50 223 1.80e-41 139
Msa0700740 AT3G58550.1 39.333 150 88 2 8 154 10 159 2.93e-33 117
Msa0700740 AT3G22600.1 32.967 91 60 1 15 105 7 96 6.32e-14 67.0
Msa0700740 AT2G48130.2 35.000 100 63 2 15 113 9 107 1.78e-13 65.9
Msa0700740 AT2G48130.6 35.000 100 63 2 15 113 9 107 1.78e-13 65.9
Msa0700740 AT2G48130.4 35.000 100 63 2 15 113 9 107 1.78e-13 65.9
Msa0700740 AT2G48130.5 35.000 100 63 2 15 113 9 107 1.78e-13 65.9
Msa0700740 AT2G48130.1 35.000 100 63 2 15 113 9 107 1.78e-13 65.9
Msa0700740 AT2G48130.3 35.000 100 63 2 15 113 9 107 1.78e-13 65.9
Msa0700740 AT1G73890.1 36.471 85 54 0 29 113 23 107 4.13e-13 65.1
Msa0700740 AT4G14815.1 35.106 94 57 2 15 108 8 97 9.64e-12 60.8
Msa0700740 AT1G03103.1 32.967 91 57 1 15 105 8 94 3.90e-11 59.3
Msa0700740 AT1G05450.2 29.448 163 103 4 13 169 10 166 6.67e-11 59.3

Find 65 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACTAATTGATCCATAAA+AGG 0.152918 4_4:+71893321 Msa0700740:three_prime_UTR
ATTGTCTGTTTCCTTTAATT+AGG 0.199180 4_4:+71893260 Msa0700740:three_prime_UTR
ACCTTAGCCTCATGTGATTT+AGG 0.238066 4_4:-71891664 None:intergenic
ACAATCTATGGTTGGAGATT+TGG 0.249161 4_4:-71891372 None:intergenic
CTATTTCATCATCTTCAAAA+TGG 0.259507 4_4:+71891193 Msa0700740:exon
TACAATCCTTTGGTAACTTA+AGG 0.268613 4_4:-71891502 None:intergenic
GACACCAGTGCAACAATCTA+TGG 0.281431 4_4:-71891384 None:intergenic
TTGTCTGTTTCCTTTAATTA+GGG 0.287901 4_4:+71893261 Msa0700740:three_prime_UTR
ACCAATACATTGAGTTATAT+TGG 0.304514 4_4:-71891534 None:intergenic
TTTATAGTTAAACCAAGGTT+TGG 0.345145 4_4:-71891467 None:intergenic
GAAGGATGGACCCTAATTAA+AGG 0.348230 4_4:-71893271 None:intergenic
GGTCATCATGATCTTTGATA+AGG 0.380512 4_4:-71891445 None:intergenic
ACAATCCTTTGGTAACTTAA+GGG 0.380953 4_4:-71891501 None:intergenic
GACAAGAACGGTGGCGGTTG+TGG 0.396527 4_4:+71893044 Msa0700740:CDS
CGGTGCATTCTGCTTTGTCT+TGG 0.411232 4_4:-71891301 None:intergenic
GACAAACTCCTTACTTTGGC+TGG 0.412680 4_4:+71891323 Msa0700740:CDS
GTAGGTGAGTTACAATCCTT+TGG 0.416024 4_4:-71891512 None:intergenic
TATTTCATCATCTTCAAAAT+GGG 0.420364 4_4:+71891194 Msa0700740:exon
TTATAGTTAAACCAAGGTTT+GGG 0.422627 4_4:-71891466 None:intergenic
AAATGTCAACATTTAATAAG+AGG 0.437356 4_4:+71893156 Msa0700740:three_prime_UTR
CTCTTGGTAGAGGTGCTTTG+TGG 0.443821 4_4:+71893077 Msa0700740:CDS
GATATATTATTGCTATATAT+CGG 0.447365 4_4:+71893178 Msa0700740:three_prime_UTR
CAACTAATTGATCCATAAAA+GGG 0.451943 4_4:+71893322 Msa0700740:three_prime_UTR
ACCAATATAACTCAATGTAT+TGG 0.457228 4_4:+71891533 Msa0700740:CDS
CACCGACAAACTCCTTACTT+TGG 0.466376 4_4:+71891319 Msa0700740:CDS
GAAGACAACCAGCCAAAGTA+AGG 0.470397 4_4:-71891331 None:intergenic
ACATGAGGCTAAGGTATTTG+AGG 0.480429 4_4:+71891672 Msa0700740:CDS
ACCTAAATCACATGAGGCTA+AGG 0.495814 4_4:+71891663 Msa0700740:CDS
TTTGTCTTGGTTAATATCTG+AGG 0.495906 4_4:-71891288 None:intergenic
CGACCTTGATTTCTAACTAC+GGG 0.496240 4_4:-71893346 None:intergenic
AAGTAGGAAACTAAAACAAA+TGG 0.516446 4_4:-71893107 None:intergenic
AAAGAGGTGGCTCTTGGTAG+AGG 0.525495 4_4:+71893067 Msa0700740:CDS
ACTTCCACCGCAGTTCCTCC+AGG 0.529242 4_4:+71891721 Msa0700740:CDS
CATAAGGACTAGGAAAATCA+AGG 0.531221 4_4:-71891252 None:intergenic
ACGACCTTGATTTCTAACTA+CGG 0.531686 4_4:-71893347 None:intergenic
GATCATGATGACCCAAACCT+TGG 0.532203 4_4:+71891455 Msa0700740:CDS
TTGGCTTGATTAGTCACAAA+GGG 0.535509 4_4:-71891353 None:intergenic
TTGTGGAAAGAGGTGGCTCT+TGG 0.535765 4_4:+71893061 Msa0700740:CDS
GGTCCCGTAGTTAGAAATCA+AGG 0.543201 4_4:+71893343 Msa0700740:three_prime_UTR
AATTCAAACTACGAAGAAGT+AGG 0.544914 4_4:-71893123 None:intergenic
CTTTGGTAACTTAAGGGCAA+GGG 0.546332 4_4:-71891495 None:intergenic
CCTTTGGTAACTTAAGGGCA+AGG 0.546657 4_4:-71891496 None:intergenic
AGGTTACAAGACCAAACATA+AGG 0.547469 4_4:-71891268 None:intergenic
CCAACCATAGATTGTTGCAC+TGG 0.548725 4_4:+71891380 Msa0700740:CDS
TGTGCTTGTTTCATTTCCAG+TGG 0.551525 4_4:-71893012 None:intergenic
ATTGCAGCTAGTGGTACCAC+TGG 0.560811 4_4:+71892996 Msa0700740:intron
CCTTGCCCTTAAGTTACCAA+AGG 0.561963 4_4:+71891496 Msa0700740:CDS
ACGGTGGCGGTTGTGGAAAG+AGG 0.565669 4_4:+71893051 Msa0700740:CDS
CAAGACCAAACATAAGGACT+AGG 0.566810 4_4:-71891262 None:intergenic
AGCCAAAGTAAGGAGTTTGT+CGG 0.567852 4_4:-71891321 None:intergenic
TAACACAGACAAGGAAAGGA+AGG 0.575654 4_4:-71893289 None:intergenic
TCAATAACACAGACAAGGAA+AGG 0.576335 4_4:-71893293 None:intergenic
ATACATTGAGTTATATTGGT+AGG 0.586657 4_4:-71891530 None:intergenic
GCTCAAGACAAGAACGGTGG+CGG 0.587172 4_4:+71893038 Msa0700740:CDS
ACAGACAAGGAAAGGAAGGA+TGG 0.594898 4_4:-71893285 None:intergenic
CCAGTGCAACAATCTATGGT+TGG 0.604106 4_4:-71891380 None:intergenic
GTGGCGGTTGTGGAAAGAGG+TGG 0.614207 4_4:+71893054 Msa0700740:CDS
TAACATTTATAGTTAAACCA+AGG 0.621795 4_4:-71891472 None:intergenic
TTTGGCTTGATTAGTCACAA+AGG 0.627914 4_4:-71891354 None:intergenic
TTTGAAACCTAAATCACATG+AGG 0.643872 4_4:+71891657 Msa0700740:CDS
TTTACCTGGAGGAACTGCGG+TGG 0.660595 4_4:-71891725 None:intergenic
AAATTTACCTGGAGGAACTG+CGG 0.666666 4_4:-71891728 None:intergenic
ACAATTGCTCAAGACAAGAA+CGG 0.667135 4_4:+71893032 Msa0700740:CDS
TTGTTGCACTGGTGTCAAAG+AGG 0.679195 4_4:+71891391 Msa0700740:CDS
ATTGCTCAAGACAAGAACGG+TGG 0.760005 4_4:+71893035 Msa0700740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTATTAAAATATTTA+GGG + chr4_4:71892413-71892432 Msa0700740:intron 0.0%
!!! ATATTTTATTAAAATATTTA+GGG + chr4_4:71892413-71892432 Msa0700740:intron 0.0%
!!! TTCTTTTTATTTATGTTATA+AGG - chr4_4:71891153-71891172 None:intergenic 10.0%
!! ATATATACATTATTATGTAA+TGG - chr4_4:71892071-71892090 None:intergenic 10.0%
!! TTTAAAAATTAAATGAAGTA+TGG + chr4_4:71892349-71892368 Msa0700740:intron 10.0%
!!! ATTTTGTATAATTTCATATT+AGG + chr4_4:71892482-71892501 Msa0700740:intron 10.0%
!! AATTAAATATCAAATTTAAC+AGG - chr4_4:71892539-71892558 None:intergenic 10.0%
!!! AATTTCTTTTTTTAAAGTTA+GGG - chr4_4:71892722-71892741 None:intergenic 10.0%
!!! TTCTTTTTATTTATGTTATA+AGG - chr4_4:71891153-71891172 None:intergenic 10.0%
!! ATATATACATTATTATGTAA+TGG - chr4_4:71892071-71892090 None:intergenic 10.0%
!! TTTAAAAATTAAATGAAGTA+TGG + chr4_4:71892349-71892368 Msa0700740:intron 10.0%
!!! ATTTTGTATAATTTCATATT+AGG + chr4_4:71892482-71892501 Msa0700740:intron 10.0%
!! AATTAAATATCAAATTTAAC+AGG - chr4_4:71892539-71892558 None:intergenic 10.0%
!!! AATTTCTTTTTTTAAAGTTA+GGG - chr4_4:71892722-71892741 None:intergenic 10.0%
!! ATAAATAAAAAGAAAACAAG+AGG + chr4_4:71891159-71891178 Msa0700740:five_prime_UTR 15.0%
!! AATAGTAAATAAAATTTACC+TGG - chr4_4:71891742-71891761 None:intergenic 15.0%
!!! CAATTTCTTTTTTTAAAGTT+AGG - chr4_4:71892723-71892742 None:intergenic 15.0%
!! GATATATTATTGCTATATAT+CGG + chr4_4:71893178-71893197 Msa0700740:three_prime_UTR 15.0%
!! ATAAATAAAAAGAAAACAAG+AGG + chr4_4:71891159-71891178 Msa0700740:five_prime_UTR 15.0%
!! AATAGTAAATAAAATTTACC+TGG - chr4_4:71891742-71891761 None:intergenic 15.0%
!!! CAATTTCTTTTTTTAAAGTT+AGG - chr4_4:71892723-71892742 None:intergenic 15.0%
!! GATATATTATTGCTATATAT+CGG + chr4_4:71893178-71893197 Msa0700740:three_prime_UTR 15.0%
!! TATTTCATCATCTTCAAAAT+GGG + chr4_4:71891194-71891213 Msa0700740:exon 20.0%
!! TAACATTTATAGTTAAACCA+AGG - chr4_4:71891475-71891494 None:intergenic 20.0%
!!! TGAAACAAATATCAAACTTT+TGG - chr4_4:71891626-71891645 None:intergenic 20.0%
!! AATATAGAAACAAAAGACTA+AGG + chr4_4:71891781-71891800 Msa0700740:intron 20.0%
!!! TTTTCTATCACATAATAAAG+TGG + chr4_4:71891925-71891944 Msa0700740:intron 20.0%
!!! TTTTCTATCACATAATAAAG+TGG + chr4_4:71891925-71891944 Msa0700740:intron 20.0%
!! TTATTAAAGATCATATAGTG+TGG - chr4_4:71892126-71892145 None:intergenic 20.0%
!!! CACTATTTTTATTGACATTT+GGG - chr4_4:71892185-71892204 None:intergenic 20.0%
!!! TCACTATTTTTATTGACATT+TGG - chr4_4:71892186-71892205 None:intergenic 20.0%
!! GTTTGAAGAGAATAAAAATT+AGG + chr4_4:71892504-71892523 Msa0700740:intron 20.0%
!! AAATAGATTATGAAAATGTG+TGG + chr4_4:71892621-71892640 Msa0700740:intron 20.0%
!! AATAGATTATGAAAATGTGT+GGG + chr4_4:71892622-71892641 Msa0700740:intron 20.0%
!! AAAAAAAGAAATTGATGTAG+AGG + chr4_4:71892729-71892748 Msa0700740:intron 20.0%
!!! ACAAATAAAAAGTATCATTG+GGG - chr4_4:71892811-71892830 None:intergenic 20.0%
!!! GACAAATAAAAAGTATCATT+GGG - chr4_4:71892812-71892831 None:intergenic 20.0%
!!! TGACAAATAAAAAGTATCAT+TGG - chr4_4:71892813-71892832 None:intergenic 20.0%
!!! TATTTTTGGATAAAAGTGAT+TGG + chr4_4:71892957-71892976 Msa0700740:intron 20.0%
!! AAATGTCAACATTTAATAAG+AGG + chr4_4:71893156-71893175 Msa0700740:three_prime_UTR 20.0%
!!! ATATTTTCGTGATGATAAAT+AGG + chr4_4:71893383-71893402 Msa0700740:three_prime_UTR 20.0%
!! TATTTCATCATCTTCAAAAT+GGG + chr4_4:71891194-71891213 Msa0700740:exon 20.0%
!! TAACATTTATAGTTAAACCA+AGG - chr4_4:71891475-71891494 None:intergenic 20.0%
!!! TGAAACAAATATCAAACTTT+TGG - chr4_4:71891626-71891645 None:intergenic 20.0%
!! AATATAGAAACAAAAGACTA+AGG + chr4_4:71891781-71891800 Msa0700740:intron 20.0%
!!! TTTTCTATCACATAATAAAG+TGG + chr4_4:71891925-71891944 Msa0700740:intron 20.0%
!!! TTTTCTATCACATAATAAAG+TGG + chr4_4:71891925-71891944 Msa0700740:intron 20.0%
!! TTATTAAAGATCATATAGTG+TGG - chr4_4:71892126-71892145 None:intergenic 20.0%
!!! CACTATTTTTATTGACATTT+GGG - chr4_4:71892185-71892204 None:intergenic 20.0%
!!! TCACTATTTTTATTGACATT+TGG - chr4_4:71892186-71892205 None:intergenic 20.0%
!! GTTTGAAGAGAATAAAAATT+AGG + chr4_4:71892504-71892523 Msa0700740:intron 20.0%
!! AAATAGATTATGAAAATGTG+TGG + chr4_4:71892621-71892640 Msa0700740:intron 20.0%
!! AATAGATTATGAAAATGTGT+GGG + chr4_4:71892622-71892641 Msa0700740:intron 20.0%
!! AAAAAAAGAAATTGATGTAG+AGG + chr4_4:71892729-71892748 Msa0700740:intron 20.0%
!!! ACAAATAAAAAGTATCATTG+GGG - chr4_4:71892811-71892830 None:intergenic 20.0%
!!! GACAAATAAAAAGTATCATT+GGG - chr4_4:71892812-71892831 None:intergenic 20.0%
!!! TGACAAATAAAAAGTATCAT+TGG - chr4_4:71892813-71892832 None:intergenic 20.0%
!!! TATTTTTGGATAAAAGTGAT+TGG + chr4_4:71892957-71892976 Msa0700740:intron 20.0%
!! AAATGTCAACATTTAATAAG+AGG + chr4_4:71893156-71893175 Msa0700740:three_prime_UTR 20.0%
!!! ATATTTTCGTGATGATAAAT+AGG + chr4_4:71893383-71893402 Msa0700740:three_prime_UTR 20.0%
! CTATTTCATCATCTTCAAAA+TGG + chr4_4:71891193-71891212 Msa0700740:exon 25.0%
! TTATAGTTAAACCAAGGTTT+GGG - chr4_4:71891469-71891488 None:intergenic 25.0%
! TTTATAGTTAAACCAAGGTT+TGG - chr4_4:71891470-71891489 None:intergenic 25.0%
! ATACATTGAGTTATATTGGT+AGG - chr4_4:71891533-71891552 None:intergenic 25.0%
! ACCAATATAACTCAATGTAT+TGG + chr4_4:71891533-71891552 Msa0700740:CDS 25.0%
! ACCAATACATTGAGTTATAT+TGG - chr4_4:71891537-71891556 None:intergenic 25.0%
!! TATTTTTCTCCAAGGATTTT+TGG - chr4_4:71891702-71891721 None:intergenic 25.0%
! AGTAAATAAAATTTACCTGG+AGG - chr4_4:71891739-71891758 None:intergenic 25.0%
!!! TCCTTGTATTTTACTGATTT+GGG - chr4_4:71891816-71891835 None:intergenic 25.0%
!!! TTCCTTGTATTTTACTGATT+TGG - chr4_4:71891817-71891836 None:intergenic 25.0%
!! ATCTCATTTGATTTCATTCT+AGG + chr4_4:71892900-71892919 Msa0700740:intron 25.0%
!!! TGATCTTGCTTGTTTATTTT+TGG + chr4_4:71892943-71892962 Msa0700740:intron 25.0%
! AAGTAGGAAACTAAAACAAA+TGG - chr4_4:71893110-71893129 None:intergenic 25.0%
! ATTGTCTGTTTCCTTTAATT+AGG + chr4_4:71893260-71893279 Msa0700740:three_prime_UTR 25.0%
! TTGTCTGTTTCCTTTAATTA+GGG + chr4_4:71893261-71893280 Msa0700740:three_prime_UTR 25.0%
! AAAAATCAATAACACAGACA+AGG - chr4_4:71893301-71893320 None:intergenic 25.0%
! CAACTAATTGATCCATAAAA+GGG + chr4_4:71893322-71893341 Msa0700740:three_prime_UTR 25.0%
! AACTACGTATAAAAATAGAG+AGG - chr4_4:71893410-71893429 None:intergenic 25.0%
! CTATTTCATCATCTTCAAAA+TGG + chr4_4:71891193-71891212 Msa0700740:exon 25.0%
! TTATAGTTAAACCAAGGTTT+GGG - chr4_4:71891469-71891488 None:intergenic 25.0%
! TTTATAGTTAAACCAAGGTT+TGG - chr4_4:71891470-71891489 None:intergenic 25.0%
! ATACATTGAGTTATATTGGT+AGG - chr4_4:71891533-71891552 None:intergenic 25.0%
! ACCAATATAACTCAATGTAT+TGG + chr4_4:71891533-71891552 Msa0700740:CDS 25.0%
! ACCAATACATTGAGTTATAT+TGG - chr4_4:71891537-71891556 None:intergenic 25.0%
!! TATTTTTCTCCAAGGATTTT+TGG - chr4_4:71891702-71891721 None:intergenic 25.0%
! AGTAAATAAAATTTACCTGG+AGG - chr4_4:71891739-71891758 None:intergenic 25.0%
!!! TCCTTGTATTTTACTGATTT+GGG - chr4_4:71891816-71891835 None:intergenic 25.0%
!!! TTCCTTGTATTTTACTGATT+TGG - chr4_4:71891817-71891836 None:intergenic 25.0%
!! ATCTCATTTGATTTCATTCT+AGG + chr4_4:71892900-71892919 Msa0700740:intron 25.0%
!!! TGATCTTGCTTGTTTATTTT+TGG + chr4_4:71892943-71892962 Msa0700740:intron 25.0%
! AAGTAGGAAACTAAAACAAA+TGG - chr4_4:71893110-71893129 None:intergenic 25.0%
! ATTGTCTGTTTCCTTTAATT+AGG + chr4_4:71893260-71893279 Msa0700740:three_prime_UTR 25.0%
! TTGTCTGTTTCCTTTAATTA+GGG + chr4_4:71893261-71893280 Msa0700740:three_prime_UTR 25.0%
! AAAAATCAATAACACAGACA+AGG - chr4_4:71893301-71893320 None:intergenic 25.0%
! CAACTAATTGATCCATAAAA+GGG + chr4_4:71893322-71893341 Msa0700740:three_prime_UTR 25.0%
! AACTACGTATAAAAATAGAG+AGG - chr4_4:71893410-71893429 None:intergenic 25.0%
! AAAAAGAAAACAAGAGGTGT+TGG + chr4_4:71891165-71891184 Msa0700740:five_prime_UTR 30.0%
! ATTTTCCTAGTCCTTATGTT+TGG + chr4_4:71891257-71891276 Msa0700740:CDS 30.0%
TTTGTCTTGGTTAATATCTG+AGG - chr4_4:71891291-71891310 None:intergenic 30.0%
! ACAATCCTTTGGTAACTTAA+GGG - chr4_4:71891504-71891523 None:intergenic 30.0%
! TACAATCCTTTGGTAACTTA+AGG - chr4_4:71891505-71891524 None:intergenic 30.0%
! TTTGAAACCTAAATCACATG+AGG + chr4_4:71891657-71891676 Msa0700740:CDS 30.0%
ACCCAAATCAGTAAAATACA+AGG + chr4_4:71891812-71891831 Msa0700740:intron 30.0%
AAAATACAAGGAAGTCAAGA+AGG + chr4_4:71891824-71891843 Msa0700740:intron 30.0%
AAATACAAGGAAGTCAAGAA+GGG + chr4_4:71891825-71891844 Msa0700740:intron 30.0%
AAGACTCGAAAAACAAATTG+AGG + chr4_4:71892783-71892802 Msa0700740:intron 30.0%
AGACTCGAAAAACAAATTGA+GGG + chr4_4:71892784-71892803 Msa0700740:intron 30.0%
TAACAAAACATGAAGACCTA+AGG - chr4_4:71892850-71892869 None:intergenic 30.0%
CTTAATAGAGATTAGAGTGA+AGG - chr4_4:71892879-71892898 None:intergenic 30.0%
AATTCATTAATTGCAGCTAG+TGG + chr4_4:71892987-71893006 Msa0700740:intron 30.0%
AATTCAAACTACGAAGAAGT+AGG - chr4_4:71893126-71893145 None:intergenic 30.0%
! CCTTTTATGGATCAATTAGT+TGG - chr4_4:71893324-71893343 None:intergenic 30.0%
CCAACTAATTGATCCATAAA+AGG + chr4_4:71893321-71893340 Msa0700740:three_prime_UTR 30.0%
! AAAAAGAAAACAAGAGGTGT+TGG + chr4_4:71891165-71891184 Msa0700740:five_prime_UTR 30.0%
! ATTTTCCTAGTCCTTATGTT+TGG + chr4_4:71891257-71891276 Msa0700740:CDS 30.0%
TTTGTCTTGGTTAATATCTG+AGG - chr4_4:71891291-71891310 None:intergenic 30.0%
! ACAATCCTTTGGTAACTTAA+GGG - chr4_4:71891504-71891523 None:intergenic 30.0%
! TACAATCCTTTGGTAACTTA+AGG - chr4_4:71891505-71891524 None:intergenic 30.0%
! TTTGAAACCTAAATCACATG+AGG + chr4_4:71891657-71891676 Msa0700740:CDS 30.0%
ACCCAAATCAGTAAAATACA+AGG + chr4_4:71891812-71891831 Msa0700740:intron 30.0%
AAAATACAAGGAAGTCAAGA+AGG + chr4_4:71891824-71891843 Msa0700740:intron 30.0%
AAATACAAGGAAGTCAAGAA+GGG + chr4_4:71891825-71891844 Msa0700740:intron 30.0%
AAGACTCGAAAAACAAATTG+AGG + chr4_4:71892783-71892802 Msa0700740:intron 30.0%
AGACTCGAAAAACAAATTGA+GGG + chr4_4:71892784-71892803 Msa0700740:intron 30.0%
TAACAAAACATGAAGACCTA+AGG - chr4_4:71892850-71892869 None:intergenic 30.0%
CTTAATAGAGATTAGAGTGA+AGG - chr4_4:71892879-71892898 None:intergenic 30.0%
AATTCATTAATTGCAGCTAG+TGG + chr4_4:71892987-71893006 Msa0700740:intron 30.0%
AATTCAAACTACGAAGAAGT+AGG - chr4_4:71893126-71893145 None:intergenic 30.0%
! CCTTTTATGGATCAATTAGT+TGG - chr4_4:71893324-71893343 None:intergenic 30.0%
CCAACTAATTGATCCATAAA+AGG + chr4_4:71893321-71893340 Msa0700740:three_prime_UTR 30.0%
CATAAGGACTAGGAAAATCA+AGG - chr4_4:71891255-71891274 None:intergenic 35.0%
AGGTTACAAGACCAAACATA+AGG - chr4_4:71891271-71891290 None:intergenic 35.0%
! TTGGCTTGATTAGTCACAAA+GGG - chr4_4:71891356-71891375 None:intergenic 35.0%
!! TTTGGCTTGATTAGTCACAA+AGG - chr4_4:71891357-71891376 None:intergenic 35.0%
ACAATCTATGGTTGGAGATT+TGG - chr4_4:71891375-71891394 None:intergenic 35.0%
GGTCATCATGATCTTTGATA+AGG - chr4_4:71891448-71891467 None:intergenic 35.0%
!! TTTACTGATTTGGGTCATGT+GGG - chr4_4:71891807-71891826 None:intergenic 35.0%
!!! TTTTACTGATTTGGGTCATG+TGG - chr4_4:71891808-71891827 None:intergenic 35.0%
CCTAGGCTTGTATATATCAA+CGG + chr4_4:71891865-71891884 Msa0700740:intron 35.0%
CTAGGCTTGTATATATCAAC+GGG + chr4_4:71891866-71891885 Msa0700740:intron 35.0%
! AGCTTAGCATTGAAACTTCA+AGG + chr4_4:71892674-71892693 Msa0700740:intron 35.0%
ACAATTGCTCAAGACAAGAA+CGG + chr4_4:71893032-71893051 Msa0700740:CDS 35.0%
TCAATAACACAGACAAGGAA+AGG - chr4_4:71893296-71893315 None:intergenic 35.0%
ACGACCTTGATTTCTAACTA+CGG - chr4_4:71893350-71893369 None:intergenic 35.0%
GTGACTTTATATTGCGTCAA+AGG - chr4_4:71891103-71891122 None:intergenic 35.0%
CATAAGGACTAGGAAAATCA+AGG - chr4_4:71891255-71891274 None:intergenic 35.0%
AGGTTACAAGACCAAACATA+AGG - chr4_4:71891271-71891290 None:intergenic 35.0%
! TTGGCTTGATTAGTCACAAA+GGG - chr4_4:71891356-71891375 None:intergenic 35.0%
!! TTTGGCTTGATTAGTCACAA+AGG - chr4_4:71891357-71891376 None:intergenic 35.0%
ACAATCTATGGTTGGAGATT+TGG - chr4_4:71891375-71891394 None:intergenic 35.0%
GGTCATCATGATCTTTGATA+AGG - chr4_4:71891448-71891467 None:intergenic 35.0%
!! TTTACTGATTTGGGTCATGT+GGG - chr4_4:71891807-71891826 None:intergenic 35.0%
!!! TTTTACTGATTTGGGTCATG+TGG - chr4_4:71891808-71891827 None:intergenic 35.0%
CCTAGGCTTGTATATATCAA+CGG + chr4_4:71891865-71891884 Msa0700740:intron 35.0%
CTAGGCTTGTATATATCAAC+GGG + chr4_4:71891866-71891885 Msa0700740:intron 35.0%
! AGCTTAGCATTGAAACTTCA+AGG + chr4_4:71892674-71892693 Msa0700740:intron 35.0%
ACAATTGCTCAAGACAAGAA+CGG + chr4_4:71893032-71893051 Msa0700740:CDS 35.0%
TCAATAACACAGACAAGGAA+AGG - chr4_4:71893296-71893315 None:intergenic 35.0%
ACGACCTTGATTTCTAACTA+CGG - chr4_4:71893350-71893369 None:intergenic 35.0%
CAAGACCAAACATAAGGACT+AGG - chr4_4:71891265-71891284 None:intergenic 40.0%
AGCCAAAGTAAGGAGTTTGT+CGG - chr4_4:71891324-71891343 None:intergenic 40.0%
! CTTTGGTAACTTAAGGGCAA+GGG - chr4_4:71891498-71891517 None:intergenic 40.0%
GTAGGTGAGTTACAATCCTT+TGG - chr4_4:71891515-71891534 None:intergenic 40.0%
ACCTAAATCACATGAGGCTA+AGG + chr4_4:71891663-71891682 Msa0700740:CDS 40.0%
ACCTTAGCCTCATGTGATTT+AGG - chr4_4:71891667-71891686 None:intergenic 40.0%
! ACATGAGGCTAAGGTATTTG+AGG + chr4_4:71891672-71891691 Msa0700740:CDS 40.0%
TGAGGACTTCCAAAAATCCT+TGG + chr4_4:71891690-71891709 Msa0700740:CDS 40.0%
!!! GGTGGAAGTATTTTTCTCCA+AGG - chr4_4:71891710-71891729 None:intergenic 40.0%
AAATTTACCTGGAGGAACTG+CGG - chr4_4:71891731-71891750 None:intergenic 40.0%
GATATATACAAGCCTAGGTG+TGG - chr4_4:71891863-71891882 None:intergenic 40.0%
CCGTTGATATATACAAGCCT+AGG - chr4_4:71891868-71891887 None:intergenic 40.0%
CAACGACTCTAAATAGCCTT+AGG + chr4_4:71892831-71892850 Msa0700740:intron 40.0%
TGTGCTTGTTTCATTTCCAG+TGG - chr4_4:71893015-71893034 None:intergenic 40.0%
GAAGGATGGACCCTAATTAA+AGG - chr4_4:71893274-71893293 None:intergenic 40.0%
TAACACAGACAAGGAAAGGA+AGG - chr4_4:71893292-71893311 None:intergenic 40.0%
CGACCTTGATTTCTAACTAC+GGG - chr4_4:71893349-71893368 None:intergenic 40.0%
CAAGACCAAACATAAGGACT+AGG - chr4_4:71891265-71891284 None:intergenic 40.0%
AGCCAAAGTAAGGAGTTTGT+CGG - chr4_4:71891324-71891343 None:intergenic 40.0%
! CTTTGGTAACTTAAGGGCAA+GGG - chr4_4:71891498-71891517 None:intergenic 40.0%
GTAGGTGAGTTACAATCCTT+TGG - chr4_4:71891515-71891534 None:intergenic 40.0%
ACCTAAATCACATGAGGCTA+AGG + chr4_4:71891663-71891682 Msa0700740:CDS 40.0%
ACCTTAGCCTCATGTGATTT+AGG - chr4_4:71891667-71891686 None:intergenic 40.0%
! ACATGAGGCTAAGGTATTTG+AGG + chr4_4:71891672-71891691 Msa0700740:CDS 40.0%
TGAGGACTTCCAAAAATCCT+TGG + chr4_4:71891690-71891709 Msa0700740:CDS 40.0%
!!! GGTGGAAGTATTTTTCTCCA+AGG - chr4_4:71891710-71891729 None:intergenic 40.0%
AAATTTACCTGGAGGAACTG+CGG - chr4_4:71891731-71891750 None:intergenic 40.0%
GATATATACAAGCCTAGGTG+TGG - chr4_4:71891863-71891882 None:intergenic 40.0%
CCGTTGATATATACAAGCCT+AGG - chr4_4:71891868-71891887 None:intergenic 40.0%
CAACGACTCTAAATAGCCTT+AGG + chr4_4:71892831-71892850 Msa0700740:intron 40.0%
TGTGCTTGTTTCATTTCCAG+TGG - chr4_4:71893015-71893034 None:intergenic 40.0%
GAAGGATGGACCCTAATTAA+AGG - chr4_4:71893274-71893293 None:intergenic 40.0%
TAACACAGACAAGGAAAGGA+AGG - chr4_4:71893292-71893311 None:intergenic 40.0%
CGACCTTGATTTCTAACTAC+GGG - chr4_4:71893349-71893368 None:intergenic 40.0%
CACCGACAAACTCCTTACTT+TGG + chr4_4:71891319-71891338 Msa0700740:CDS 45.0%
GACAAACTCCTTACTTTGGC+TGG + chr4_4:71891323-71891342 Msa0700740:CDS 45.0%
GAAGACAACCAGCCAAAGTA+AGG - chr4_4:71891334-71891353 None:intergenic 45.0%
CCAGTGCAACAATCTATGGT+TGG - chr4_4:71891383-71891402 None:intergenic 45.0%
CCAACCATAGATTGTTGCAC+TGG + chr4_4:71891380-71891399 Msa0700740:CDS 45.0%
GACACCAGTGCAACAATCTA+TGG - chr4_4:71891387-71891406 None:intergenic 45.0%
! TTGTTGCACTGGTGTCAAAG+AGG + chr4_4:71891391-71891410 Msa0700740:CDS 45.0%
GATCATGATGACCCAAACCT+TGG + chr4_4:71891455-71891474 Msa0700740:CDS 45.0%
! CCTTTGGTAACTTAAGGGCA+AGG - chr4_4:71891499-71891518 None:intergenic 45.0%
CCTTGCCCTTAAGTTACCAA+AGG + chr4_4:71891496-71891515 Msa0700740:CDS 45.0%
TACAAGGAAGTCAAGAAGGG+TGG + chr4_4:71891828-71891847 Msa0700740:intron 45.0%
TGGAATTTCCATCCACACCT+AGG + chr4_4:71891848-71891867 Msa0700740:intron 45.0%
TATACAAGCCTAGGTGTGGA+TGG - chr4_4:71891859-71891878 None:intergenic 45.0%
GTTAATCGAGCTGCACTTAC+TGG + chr4_4:71892099-71892118 Msa0700740:intron 45.0%
ATTGCTCAAGACAAGAACGG+TGG + chr4_4:71893035-71893054 Msa0700740:CDS 45.0%
ACAGACAAGGAAAGGAAGGA+TGG - chr4_4:71893288-71893307 None:intergenic 45.0%
! CTAACTACGGGACCCTTTTA+TGG - chr4_4:71893337-71893356 None:intergenic 45.0%
GGTCCCGTAGTTAGAAATCA+AGG + chr4_4:71893343-71893362 Msa0700740:three_prime_UTR 45.0%
CACCGACAAACTCCTTACTT+TGG + chr4_4:71891319-71891338 Msa0700740:CDS 45.0%
GACAAACTCCTTACTTTGGC+TGG + chr4_4:71891323-71891342 Msa0700740:CDS 45.0%
GAAGACAACCAGCCAAAGTA+AGG - chr4_4:71891334-71891353 None:intergenic 45.0%
CCAGTGCAACAATCTATGGT+TGG - chr4_4:71891383-71891402 None:intergenic 45.0%
CCAACCATAGATTGTTGCAC+TGG + chr4_4:71891380-71891399 Msa0700740:CDS 45.0%
GACACCAGTGCAACAATCTA+TGG - chr4_4:71891387-71891406 None:intergenic 45.0%
! TTGTTGCACTGGTGTCAAAG+AGG + chr4_4:71891391-71891410 Msa0700740:CDS 45.0%
GATCATGATGACCCAAACCT+TGG + chr4_4:71891455-71891474 Msa0700740:CDS 45.0%
! CCTTTGGTAACTTAAGGGCA+AGG - chr4_4:71891499-71891518 None:intergenic 45.0%
CCTTGCCCTTAAGTTACCAA+AGG + chr4_4:71891496-71891515 Msa0700740:CDS 45.0%
TACAAGGAAGTCAAGAAGGG+TGG + chr4_4:71891828-71891847 Msa0700740:intron 45.0%
TGGAATTTCCATCCACACCT+AGG + chr4_4:71891848-71891867 Msa0700740:intron 45.0%
TATACAAGCCTAGGTGTGGA+TGG - chr4_4:71891859-71891878 None:intergenic 45.0%
GTTAATCGAGCTGCACTTAC+TGG + chr4_4:71892099-71892118 Msa0700740:intron 45.0%
ATTGCTCAAGACAAGAACGG+TGG + chr4_4:71893035-71893054 Msa0700740:CDS 45.0%
ACAGACAAGGAAAGGAAGGA+TGG - chr4_4:71893288-71893307 None:intergenic 45.0%
! CTAACTACGGGACCCTTTTA+TGG - chr4_4:71893337-71893356 None:intergenic 45.0%
GGTCCCGTAGTTAGAAATCA+AGG + chr4_4:71893343-71893362 Msa0700740:three_prime_UTR 45.0%
!!! CATATTTTATTAAAATATTT+AGG + chr4_4:71892412-71892431 Msa0700740:intron 5.0%
!!! CATATTTTATTAAAATATTT+AGG + chr4_4:71892412-71892431 Msa0700740:intron 5.0%
CGGTGCATTCTGCTTTGTCT+TGG - chr4_4:71891304-71891323 None:intergenic 50.0%
ATTGCAGCTAGTGGTACCAC+TGG + chr4_4:71892996-71893015 Msa0700740:intron 50.0%
TTGTGGAAAGAGGTGGCTCT+TGG + chr4_4:71893061-71893080 Msa0700740:CDS 50.0%
AAAGAGGTGGCTCTTGGTAG+AGG + chr4_4:71893067-71893086 Msa0700740:CDS 50.0%
!! CTCTTGGTAGAGGTGCTTTG+TGG + chr4_4:71893077-71893096 Msa0700740:CDS 50.0%
CGGTGCATTCTGCTTTGTCT+TGG - chr4_4:71891304-71891323 None:intergenic 50.0%
ATTGCAGCTAGTGGTACCAC+TGG + chr4_4:71892996-71893015 Msa0700740:intron 50.0%
TTGTGGAAAGAGGTGGCTCT+TGG + chr4_4:71893061-71893080 Msa0700740:CDS 50.0%
AAAGAGGTGGCTCTTGGTAG+AGG + chr4_4:71893067-71893086 Msa0700740:CDS 50.0%
!! CTCTTGGTAGAGGTGCTTTG+TGG + chr4_4:71893077-71893096 Msa0700740:CDS 50.0%
TTTACCTGGAGGAACTGCGG+TGG - chr4_4:71891728-71891747 None:intergenic 55.0%
GCTCAAGACAAGAACGGTGG+CGG + chr4_4:71893038-71893057 Msa0700740:CDS 55.0%
TTTACCTGGAGGAACTGCGG+TGG - chr4_4:71891728-71891747 None:intergenic 55.0%
GCTCAAGACAAGAACGGTGG+CGG + chr4_4:71893038-71893057 Msa0700740:CDS 55.0%
ACTTCCACCGCAGTTCCTCC+AGG + chr4_4:71891721-71891740 Msa0700740:CDS 60.0%
! GACAAGAACGGTGGCGGTTG+TGG + chr4_4:71893044-71893063 Msa0700740:CDS 60.0%
! ACGGTGGCGGTTGTGGAAAG+AGG + chr4_4:71893051-71893070 Msa0700740:CDS 60.0%
GTGGCGGTTGTGGAAAGAGG+TGG + chr4_4:71893054-71893073 Msa0700740:CDS 60.0%
ACTTCCACCGCAGTTCCTCC+AGG + chr4_4:71891721-71891740 Msa0700740:CDS 60.0%
! GACAAGAACGGTGGCGGTTG+TGG + chr4_4:71893044-71893063 Msa0700740:CDS 60.0%
! ACGGTGGCGGTTGTGGAAAG+AGG + chr4_4:71893051-71893070 Msa0700740:CDS 60.0%
GTGGCGGTTGTGGAAAGAGG+TGG + chr4_4:71893054-71893073 Msa0700740:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 71891103 71893437 71891103 ID=Msa0700740;Name=Msa0700740
chr4_4 mRNA 71891103 71893437 71891103 ID=Msa0700740-mRNA-1;Parent=Msa0700740;Name=Msa0700740-mRNA-1;_AED=0.06;_eAED=0.06;_QI=109|1|1|1|1|1|3|295|192
chr4_4 exon 71891103 71891554 71891103 ID=Msa0700740-mRNA-1:exon:21905;Parent=Msa0700740-mRNA-1
chr4_4 exon 71891647 71891742 71891647 ID=Msa0700740-mRNA-1:exon:21906;Parent=Msa0700740-mRNA-1
chr4_4 exon 71893003 71893437 71893003 ID=Msa0700740-mRNA-1:exon:21907;Parent=Msa0700740-mRNA-1
chr4_4 five_prime_UTR 71891103 71891211 71891103 ID=Msa0700740-mRNA-1:five_prime_utr;Parent=Msa0700740-mRNA-1
chr4_4 CDS 71891212 71891554 71891212 ID=Msa0700740-mRNA-1:cds;Parent=Msa0700740-mRNA-1
chr4_4 CDS 71891647 71891742 71891647 ID=Msa0700740-mRNA-1:cds;Parent=Msa0700740-mRNA-1
chr4_4 CDS 71893003 71893142 71893003 ID=Msa0700740-mRNA-1:cds;Parent=Msa0700740-mRNA-1
chr4_4 three_prime_UTR 71893143 71893437 71893143 ID=Msa0700740-mRNA-1:three_prime_utr;Parent=Msa0700740-mRNA-1
Gene Sequence

>Msa0700740

ATGGGTTGCAAAAGCACAACAAATCTATCACTCTTCTCTACCTTGATTTTCCTAGTCCTTATGTTTGGTCTTGTAACCTCAGATATTAACCAAGACAAAGCAGAATGCACCGACAAACTCCTTACTTTGGCTGGTTGTCTTCCCTTTGTGACTAATCAAGCCAAATCTCCAACCATAGATTGTTGCACTGGTGTCAAAGAGGTTGTTGATAAGAGTAAGAGATGTTTATGTATCCTTATCAAAGATCATGATGACCCAAACCTTGGTTTAACTATAAATGTTACCCTTGCCCTTAAGTTACCAAAGGATTGTAACTCACCTACCAATATAACTCAATGTATTGATATTTTGCATTTGAAACCTAAATCACATGAGGCTAAGGTATTTGAGGACTTCCAAAAATCCTTGGAGAAAAATACTTCCACCGCAGTTCCTCCAGCTAGTGGTACCACTGGAAATGAAACAAGCACAATTGCTCAAGACAAGAACGGTGGCGGTTGTGGAAAGAGGTGGCTCTTGGTAGAGGTGCTTTGTGGCATTTTACCATTTGTTTTAGTTTCCTACTTCTTCGTAGTTTGA
Protein sequence

>Msa0700740

MGCKSTTNLSLFSTLIFLVLMFGLVTSDINQDKAECTDKLLTLAGCLPFVTNQAKSPTIDCCTGVKEVVDKSKRCLCILIKDHDDPNLGLTINVTLALKLPKDCNSPTNITQCIDILHLKPKSHEAKVFEDFQKSLEKNTSTAVPPASGTTGNETSTIAQDKNGGGCGKRWLLVEVLCGILPFVLVSYFFVV*