AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0564460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0564460 MtrunA17_Chr8g0378461 94.845 194 8 1 1 192 1 194 4.21e-131 365
Msa0564460 MtrunA17_Chr3g0121381 51.765 170 80 1 23 192 18 185 8.83e-57 177
Msa0564460 MtrunA17_Chr8g0356191 40.230 174 100 4 15 185 13 185 5.97e-39 132
Msa0564460 MtrunA17_Chr7g0219391 45.631 103 52 1 15 113 10 112 5.01e-27 99.8
Msa0564460 MtrunA17_Chr1g0205191 40.789 76 42 1 33 108 25 97 2.87e-12 60.8
Msa0564460 MtrunA17_Chr1g0205181 37.736 106 58 3 9 113 6 104 4.55e-12 61.2
Msa0564460 MtrunA17_Chr5g0395001 28.472 144 93 4 15 157 12 146 1.86e-11 60.1
Msa0564460 MtrunA17_Chr5g0394991 34.862 109 64 4 10 113 6 112 4.67e-11 59.7
Msa0564460 MtrunA17_Chr1g0205201 39.024 82 48 2 33 113 25 105 7.38e-11 58.2
Msa0564460 MtrunA17_Chr5g0429901 32.653 147 79 6 14 149 6 143 8.24e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0564460 AT1G55260.1 42.308 182 99 3 10 190 50 226 2.37e-43 144
Msa0564460 AT1G55260.2 41.758 182 97 4 10 190 50 223 9.60e-42 140
Msa0564460 AT2G44300.1 37.255 204 108 5 6 192 3 203 2.37e-41 139
Msa0564460 AT2G44290.1 36.082 194 100 2 14 192 14 198 3.03e-41 138
Msa0564460 AT3G58550.1 42.308 130 73 1 8 135 10 139 5.48e-33 116
Msa0564460 AT3G22600.1 32.967 91 60 1 15 105 7 96 6.79e-14 67.0
Msa0564460 AT2G48130.2 28.947 152 102 4 15 164 9 156 1.82e-13 65.9
Msa0564460 AT2G48130.6 28.947 152 102 4 15 164 9 156 1.82e-13 65.9
Msa0564460 AT2G48130.4 28.947 152 102 4 15 164 9 156 1.82e-13 65.9
Msa0564460 AT2G48130.5 28.947 152 102 4 15 164 9 156 1.82e-13 65.9
Msa0564460 AT2G48130.1 28.947 152 102 4 15 164 9 156 1.82e-13 65.9
Msa0564460 AT2G48130.3 28.947 152 102 4 15 164 9 156 1.82e-13 65.9
Msa0564460 AT1G73890.1 36.471 85 54 0 29 113 23 107 4.13e-13 65.1
Msa0564460 AT4G14815.1 35.106 94 57 2 15 108 8 97 1.00e-11 60.8
Msa0564460 AT1G03103.1 32.967 91 57 1 15 105 8 94 4.11e-11 59.3
Msa0564460 AT1G05450.2 29.448 163 103 4 13 169 10 166 7.68e-11 59.3

Find 63 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACTAATTGATCCATAAA+AGG 0.152918 4_1:+65124220 Msa0564460:three_prime_UTR
ACCTTAGCCTCATGTGATTT+AGG 0.238066 4_1:-65122642 None:intergenic
ACAATCTATGGTTGGAGATT+TGG 0.249161 4_1:-65122350 None:intergenic
TACAATCCTTTGGTAACTTA+AGG 0.268613 4_1:-65122480 None:intergenic
GACACCAGTGCAACAATCTA+TGG 0.281431 4_1:-65122362 None:intergenic
ACCAATACATTGAGTTATAT+TGG 0.304514 4_1:-65122512 None:intergenic
TTTATAGTTAAACCAAGGTT+TGG 0.345145 4_1:-65122445 None:intergenic
GAAGGATGGACCCTAATTAA+AGG 0.348230 4_1:-65124170 None:intergenic
GGTCATCATGATCTTTGATA+AGG 0.380512 4_1:-65122423 None:intergenic
ACAATCCTTTGGTAACTTAA+GGG 0.380953 4_1:-65122479 None:intergenic
GACAAGAACGGTGGCGGTTG+TGG 0.396527 4_1:+65123943 Msa0564460:CDS
CGGTGCATTCTGCTTTGTCT+TGG 0.411232 4_1:-65122279 None:intergenic
GACAAACTCCTTACTTTGGC+TGG 0.412680 4_1:+65122301 Msa0564460:CDS
GTAGGTGAGTTACAATCCTT+TGG 0.416024 4_1:-65122490 None:intergenic
GCTAGTGGTACCACTGGAAA+TGG 0.420680 4_1:+65123901 Msa0564460:intron
TTATAGTTAAACCAAGGTTT+GGG 0.422627 4_1:-65122444 None:intergenic
AAATGTCGACATTTAATAAG+AGG 0.437356 4_1:+65124055 Msa0564460:three_prime_UTR
CTCTTGGTAGAGGTGCTTTG+TGG 0.443821 4_1:+65123976 Msa0564460:CDS
GATATATTATTGCTATATAT+CGG 0.447365 4_1:+65124077 Msa0564460:three_prime_UTR
CAACTAATTGATCCATAAAA+GGG 0.451943 4_1:+65124221 Msa0564460:three_prime_UTR
ACCAATATAACTCAATGTAT+TGG 0.457228 4_1:+65122511 Msa0564460:CDS
CACCGACAAACTCCTTACTT+TGG 0.466376 4_1:+65122297 Msa0564460:CDS
GAAGACAACCAGCCAAAGTA+AGG 0.470397 4_1:-65122309 None:intergenic
ACATGAGGCTAAGGTATTTG+AGG 0.480429 4_1:+65122650 Msa0564460:CDS
AAGTAGGAAACTAAAACATA+TGG 0.489773 4_1:-65124006 None:intergenic
ACCTAAATCACATGAGGCTA+AGG 0.495814 4_1:+65122641 Msa0564460:CDS
TTTGTCTTGGTTAATATCTG+AGG 0.495906 4_1:-65122266 None:intergenic
AAAAGAAAACAAAGAGGTGT+TGG 0.506777 4_1:+65122143 Msa0564460:five_prime_UTR
AAAGAGGTGGCTCTTGGTAG+AGG 0.525495 4_1:+65123966 Msa0564460:CDS
ACTTCCACCGCAGTTCCTCC+AGG 0.529242 4_1:+65122699 Msa0564460:CDS
CATAAGGACTAGGAAAATCA+AGG 0.531221 4_1:-65122230 None:intergenic
GATCATGATGACCCAAACCT+TGG 0.532203 4_1:+65122433 Msa0564460:CDS
TTGGCTTGATTAGTCACAAA+GGG 0.535509 4_1:-65122331 None:intergenic
TTGTGGAAAGAGGTGGCTCT+TGG 0.535765 4_1:+65123960 Msa0564460:CDS
AGTTCAAACTACGAAGAAGT+AGG 0.542772 4_1:-65124022 None:intergenic
CTTTGGTAACTTAAGGGCAA+GGG 0.546332 4_1:-65122473 None:intergenic
CCTTTGGTAACTTAAGGGCA+AGG 0.546657 4_1:-65122474 None:intergenic
AGGTTACAAGACCAAACATA+AGG 0.547469 4_1:-65122246 None:intergenic
CCAACCATAGATTGTTGCAC+TGG 0.548725 4_1:+65122358 Msa0564460:CDS
CGACCTTGATGTCTAACTAC+GGG 0.556444 4_1:-65124245 None:intergenic
ACGACCTTGATGTCTAACTA+CGG 0.557088 4_1:-65124246 None:intergenic
ATTGCAGCTAGTGGTACCAC+TGG 0.560811 4_1:+65123895 Msa0564460:intron
CCTTGCCCTTAAGTTACCAA+AGG 0.561963 4_1:+65122474 Msa0564460:CDS
ACGGTGGCGGTTGTGGAAAG+AGG 0.565669 4_1:+65123950 Msa0564460:CDS
CAAGACCAAACATAAGGACT+AGG 0.566810 4_1:-65122240 None:intergenic
AGCCAAAGTAAGGAGTTTGT+CGG 0.567852 4_1:-65122299 None:intergenic
TAACACAGACAAGGAAAGGA+AGG 0.575654 4_1:-65124188 None:intergenic
TCAATAACACAGACAAGGAA+AGG 0.576335 4_1:-65124192 None:intergenic
TGTGCTTGTTCCATTTCCAG+TGG 0.579541 4_1:-65123911 None:intergenic
ATACATTGAGTTATATTGGT+AGG 0.586657 4_1:-65122508 None:intergenic
GCTCAAGACAAGAACGGTGG+CGG 0.587172 4_1:+65123937 Msa0564460:CDS
GGTCCCGTAGTTAGACATCA+AGG 0.593276 4_1:+65124242 Msa0564460:three_prime_UTR
ACAGACAAGGAAAGGAAGGA+TGG 0.594898 4_1:-65124184 None:intergenic
CCAGTGCAACAATCTATGGT+TGG 0.604106 4_1:-65122358 None:intergenic
GTGGCGGTTGTGGAAAGAGG+TGG 0.614207 4_1:+65123953 Msa0564460:CDS
TAACATTTATAGTTAAACCA+AGG 0.621795 4_1:-65122450 None:intergenic
TTTGGCTTGATTAGTCACAA+AGG 0.627914 4_1:-65122332 None:intergenic
TTTGAAACCTAAATCACATG+AGG 0.643872 4_1:+65122635 Msa0564460:CDS
TTTACCTGGAGGAACTGCGG+TGG 0.660595 4_1:-65122703 None:intergenic
AAATTTACCTGGAGGAACTG+CGG 0.666666 4_1:-65122706 None:intergenic
ACAATTGCTCAAGACAAGAA+CGG 0.667135 4_1:+65123931 Msa0564460:CDS
TTGTTGCACTGGTGTCAAAG+AGG 0.679195 4_1:+65122369 Msa0564460:CDS
ATTGCTCAAGACAAGAACGG+TGG 0.760005 4_1:+65123934 Msa0564460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTATTAAAATATTTA+GGG + chr4_1:65123309-65123328 Msa0564460:intron 0.0%
!!! ATATTTTATTAAAATATTTA+GGG + chr4_1:65123309-65123328 Msa0564460:intron 0.0%
!! ATATATACATTATTATGTAA+TGG - chr4_1:65122961-65122980 None:intergenic 10.0%
!!! ATTTTGTATAATTTCATATT+AGG + chr4_1:65123378-65123397 Msa0564460:intron 10.0%
!! AATTAAATATCAAATTTAAC+AGG - chr4_1:65123434-65123453 None:intergenic 10.0%
!!! AATTTCTTTTTTTAAAGTTA+GGG - chr4_1:65123617-65123636 None:intergenic 10.0%
!! ATATATACATTATTATGTAA+TGG - chr4_1:65122961-65122980 None:intergenic 10.0%
!!! ATTTTGTATAATTTCATATT+AGG + chr4_1:65123378-65123397 Msa0564460:intron 10.0%
!! AATTAAATATCAAATTTAAC+AGG - chr4_1:65123434-65123453 None:intergenic 10.0%
!!! AATTTCTTTTTTTAAAGTTA+GGG - chr4_1:65123617-65123636 None:intergenic 10.0%
!!! TTCTTTTTATCTATGTTATA+AGG - chr4_1:65122130-65122149 None:intergenic 15.0%
!! AATAGTAAATAAAATTTACC+TGG - chr4_1:65122720-65122739 None:intergenic 15.0%
!! GTTTAAAAATTAATGAAGTA+TGG + chr4_1:65123245-65123264 Msa0564460:intron 15.0%
!!! CAATTTCTTTTTTTAAAGTT+AGG - chr4_1:65123618-65123637 None:intergenic 15.0%
!!! ACAAATAAAAAGTATCATTT+GGG - chr4_1:65123706-65123725 None:intergenic 15.0%
!! GATATATTATTGCTATATAT+CGG + chr4_1:65124077-65124096 Msa0564460:three_prime_UTR 15.0%
!!! TTCTTTTTATCTATGTTATA+AGG - chr4_1:65122130-65122149 None:intergenic 15.0%
!! AATAGTAAATAAAATTTACC+TGG - chr4_1:65122720-65122739 None:intergenic 15.0%
!! GTTTAAAAATTAATGAAGTA+TGG + chr4_1:65123245-65123264 Msa0564460:intron 15.0%
!!! CAATTTCTTTTTTTAAAGTT+AGG - chr4_1:65123618-65123637 None:intergenic 15.0%
!!! ACAAATAAAAAGTATCATTT+GGG - chr4_1:65123706-65123725 None:intergenic 15.0%
!! GATATATTATTGCTATATAT+CGG + chr4_1:65124077-65124096 Msa0564460:three_prime_UTR 15.0%
!! TAGATAAAAAGAAAACAAAG+AGG + chr4_1:65122137-65122156 Msa0564460:five_prime_UTR 20.0%
!! TAACATTTATAGTTAAACCA+AGG - chr4_1:65122453-65122472 None:intergenic 20.0%
!!! TGAAACAAATATCAAACTTT+TGG - chr4_1:65122604-65122623 None:intergenic 20.0%
!!! TTTTCTATCACATAATAAAG+TGG + chr4_1:65122879-65122898 Msa0564460:intron 20.0%
!! TTATTAAAGATCATATAGTG+TGG - chr4_1:65123016-65123035 None:intergenic 20.0%
!! GTTTGAAGAGAATAAAAATT+AGG + chr4_1:65123400-65123419 Msa0564460:intron 20.0%
!! AAATAGATTATGAAAATGTG+TGG + chr4_1:65123516-65123535 Msa0564460:intron 20.0%
!! AATAGATTATGAAAATGTGT+GGG + chr4_1:65123517-65123536 Msa0564460:intron 20.0%
!! AAAAAAAGAAATTGATGTAG+AGG + chr4_1:65123624-65123643 Msa0564460:intron 20.0%
!!! GACAAATAAAAAGTATCATT+TGG - chr4_1:65123707-65123726 None:intergenic 20.0%
!!! TATTTTTGGATAAAAGTGAT+TGG + chr4_1:65123856-65123875 Msa0564460:intron 20.0%
!!! ATTGTCTTTTTCCTTTAATT+AGG + chr4_1:65124159-65124178 Msa0564460:three_prime_UTR 20.0%
!!! TTGTCTTTTTCCTTTAATTA+GGG + chr4_1:65124160-65124179 Msa0564460:three_prime_UTR 20.0%
!! TAGATAAAAAGAAAACAAAG+AGG + chr4_1:65122137-65122156 Msa0564460:five_prime_UTR 20.0%
!! TAACATTTATAGTTAAACCA+AGG - chr4_1:65122453-65122472 None:intergenic 20.0%
!!! TGAAACAAATATCAAACTTT+TGG - chr4_1:65122604-65122623 None:intergenic 20.0%
!!! TTTTCTATCACATAATAAAG+TGG + chr4_1:65122879-65122898 Msa0564460:intron 20.0%
!! TTATTAAAGATCATATAGTG+TGG - chr4_1:65123016-65123035 None:intergenic 20.0%
!! GTTTGAAGAGAATAAAAATT+AGG + chr4_1:65123400-65123419 Msa0564460:intron 20.0%
!! AAATAGATTATGAAAATGTG+TGG + chr4_1:65123516-65123535 Msa0564460:intron 20.0%
!! AATAGATTATGAAAATGTGT+GGG + chr4_1:65123517-65123536 Msa0564460:intron 20.0%
!! AAAAAAAGAAATTGATGTAG+AGG + chr4_1:65123624-65123643 Msa0564460:intron 20.0%
!!! GACAAATAAAAAGTATCATT+TGG - chr4_1:65123707-65123726 None:intergenic 20.0%
!!! TATTTTTGGATAAAAGTGAT+TGG + chr4_1:65123856-65123875 Msa0564460:intron 20.0%
!!! ATTGTCTTTTTCCTTTAATT+AGG + chr4_1:65124159-65124178 Msa0564460:three_prime_UTR 20.0%
!!! TTGTCTTTTTCCTTTAATTA+GGG + chr4_1:65124160-65124179 Msa0564460:three_prime_UTR 20.0%
! TTATAGTTAAACCAAGGTTT+GGG - chr4_1:65122447-65122466 None:intergenic 25.0%
! TTTATAGTTAAACCAAGGTT+TGG - chr4_1:65122448-65122467 None:intergenic 25.0%
! ATACATTGAGTTATATTGGT+AGG - chr4_1:65122511-65122530 None:intergenic 25.0%
! ACCAATATAACTCAATGTAT+TGG + chr4_1:65122511-65122530 Msa0564460:CDS 25.0%
! ACCAATACATTGAGTTATAT+TGG - chr4_1:65122515-65122534 None:intergenic 25.0%
!! TATTTTTCTCCAAGGATTTT+TGG - chr4_1:65122680-65122699 None:intergenic 25.0%
! AGTAAATAAAATTTACCTGG+AGG - chr4_1:65122717-65122736 None:intergenic 25.0%
!!! TCCTTGTATTTTACTGATTT+GGG - chr4_1:65122771-65122790 None:intergenic 25.0%
!!! TTCCTTGTATTTTACTGATT+TGG - chr4_1:65122772-65122791 None:intergenic 25.0%
!!! CACTATTTTGATTGACATTT+GGG - chr4_1:65123075-65123094 None:intergenic 25.0%
!!! TCACTATTTTGATTGACATT+TGG - chr4_1:65123076-65123095 None:intergenic 25.0%
!! ATCTCATTTGATTTCATTCT+AGG + chr4_1:65123795-65123814 Msa0564460:intron 25.0%
!!! TGATCTTGCTTGTTTATTTT+TGG + chr4_1:65123842-65123861 Msa0564460:intron 25.0%
! AAGTAGGAAACTAAAACATA+TGG - chr4_1:65124009-65124028 None:intergenic 25.0%
! AAATGTCGACATTTAATAAG+AGG + chr4_1:65124055-65124074 Msa0564460:three_prime_UTR 25.0%
! AAAAATCAATAACACAGACA+AGG - chr4_1:65124200-65124219 None:intergenic 25.0%
! CAACTAATTGATCCATAAAA+GGG + chr4_1:65124221-65124240 Msa0564460:three_prime_UTR 25.0%
! TTATAGTTAAACCAAGGTTT+GGG - chr4_1:65122447-65122466 None:intergenic 25.0%
! TTTATAGTTAAACCAAGGTT+TGG - chr4_1:65122448-65122467 None:intergenic 25.0%
! ATACATTGAGTTATATTGGT+AGG - chr4_1:65122511-65122530 None:intergenic 25.0%
! ACCAATATAACTCAATGTAT+TGG + chr4_1:65122511-65122530 Msa0564460:CDS 25.0%
! ACCAATACATTGAGTTATAT+TGG - chr4_1:65122515-65122534 None:intergenic 25.0%
!! TATTTTTCTCCAAGGATTTT+TGG - chr4_1:65122680-65122699 None:intergenic 25.0%
! AGTAAATAAAATTTACCTGG+AGG - chr4_1:65122717-65122736 None:intergenic 25.0%
!!! TCCTTGTATTTTACTGATTT+GGG - chr4_1:65122771-65122790 None:intergenic 25.0%
!!! TTCCTTGTATTTTACTGATT+TGG - chr4_1:65122772-65122791 None:intergenic 25.0%
!!! CACTATTTTGATTGACATTT+GGG - chr4_1:65123075-65123094 None:intergenic 25.0%
!!! TCACTATTTTGATTGACATT+TGG - chr4_1:65123076-65123095 None:intergenic 25.0%
!! ATCTCATTTGATTTCATTCT+AGG + chr4_1:65123795-65123814 Msa0564460:intron 25.0%
!!! TGATCTTGCTTGTTTATTTT+TGG + chr4_1:65123842-65123861 Msa0564460:intron 25.0%
! AAGTAGGAAACTAAAACATA+TGG - chr4_1:65124009-65124028 None:intergenic 25.0%
! AAATGTCGACATTTAATAAG+AGG + chr4_1:65124055-65124074 Msa0564460:three_prime_UTR 25.0%
! AAAAATCAATAACACAGACA+AGG - chr4_1:65124200-65124219 None:intergenic 25.0%
! CAACTAATTGATCCATAAAA+GGG + chr4_1:65124221-65124240 Msa0564460:three_prime_UTR 25.0%
! AAAAGAAAACAAAGAGGTGT+TGG + chr4_1:65122143-65122162 Msa0564460:five_prime_UTR 30.0%
! ATTTTCCTAGTCCTTATGTT+TGG + chr4_1:65122235-65122254 Msa0564460:CDS 30.0%
TTTGTCTTGGTTAATATCTG+AGG - chr4_1:65122269-65122288 None:intergenic 30.0%
! ACAATCCTTTGGTAACTTAA+GGG - chr4_1:65122482-65122501 None:intergenic 30.0%
! TACAATCCTTTGGTAACTTA+AGG - chr4_1:65122483-65122502 None:intergenic 30.0%
! TTTGAAACCTAAATCACATG+AGG + chr4_1:65122635-65122654 Msa0564460:CDS 30.0%
ACCCAAATCAGTAAAATACA+AGG + chr4_1:65122767-65122786 Msa0564460:intron 30.0%
AAAATACAAGGAAGTCAAGA+AGG + chr4_1:65122779-65122798 Msa0564460:intron 30.0%
AAATACAAGGAAGTCAAGAA+GGG + chr4_1:65122780-65122799 Msa0564460:intron 30.0%
CCTAGGTTTGTATATATCAA+CGG + chr4_1:65122820-65122839 Msa0564460:intron 30.0%
CTAGGTTTGTATATATCAAC+GGG + chr4_1:65122821-65122840 Msa0564460:intron 30.0%
AAGACTCGAAAAACAAATTG+AGG + chr4_1:65123678-65123697 Msa0564460:intron 30.0%
AGACTCGAAAAACAAATTGA+GGG + chr4_1:65123679-65123698 Msa0564460:intron 30.0%
TAACAAAACATGAAGACCTA+AGG - chr4_1:65123745-65123764 None:intergenic 30.0%
CTTAATAGAGATTAGAGTGA+AGG - chr4_1:65123774-65123793 None:intergenic 30.0%
AATTCATTAATTGCAGCTAG+TGG + chr4_1:65123886-65123905 Msa0564460:intron 30.0%
! CCTTTTATGGATCAATTAGT+TGG - chr4_1:65124223-65124242 None:intergenic 30.0%
CCAACTAATTGATCCATAAA+AGG + chr4_1:65124220-65124239 Msa0564460:three_prime_UTR 30.0%
! AAAAGAAAACAAAGAGGTGT+TGG + chr4_1:65122143-65122162 Msa0564460:five_prime_UTR 30.0%
! ATTTTCCTAGTCCTTATGTT+TGG + chr4_1:65122235-65122254 Msa0564460:CDS 30.0%
TTTGTCTTGGTTAATATCTG+AGG - chr4_1:65122269-65122288 None:intergenic 30.0%
! ACAATCCTTTGGTAACTTAA+GGG - chr4_1:65122482-65122501 None:intergenic 30.0%
! TACAATCCTTTGGTAACTTA+AGG - chr4_1:65122483-65122502 None:intergenic 30.0%
! TTTGAAACCTAAATCACATG+AGG + chr4_1:65122635-65122654 Msa0564460:CDS 30.0%
ACCCAAATCAGTAAAATACA+AGG + chr4_1:65122767-65122786 Msa0564460:intron 30.0%
AAAATACAAGGAAGTCAAGA+AGG + chr4_1:65122779-65122798 Msa0564460:intron 30.0%
AAATACAAGGAAGTCAAGAA+GGG + chr4_1:65122780-65122799 Msa0564460:intron 30.0%
CCTAGGTTTGTATATATCAA+CGG + chr4_1:65122820-65122839 Msa0564460:intron 30.0%
CTAGGTTTGTATATATCAAC+GGG + chr4_1:65122821-65122840 Msa0564460:intron 30.0%
AAGACTCGAAAAACAAATTG+AGG + chr4_1:65123678-65123697 Msa0564460:intron 30.0%
AGACTCGAAAAACAAATTGA+GGG + chr4_1:65123679-65123698 Msa0564460:intron 30.0%
TAACAAAACATGAAGACCTA+AGG - chr4_1:65123745-65123764 None:intergenic 30.0%
CTTAATAGAGATTAGAGTGA+AGG - chr4_1:65123774-65123793 None:intergenic 30.0%
AATTCATTAATTGCAGCTAG+TGG + chr4_1:65123886-65123905 Msa0564460:intron 30.0%
! CCTTTTATGGATCAATTAGT+TGG - chr4_1:65124223-65124242 None:intergenic 30.0%
CCAACTAATTGATCCATAAA+AGG + chr4_1:65124220-65124239 Msa0564460:three_prime_UTR 30.0%
CATAAGGACTAGGAAAATCA+AGG - chr4_1:65122233-65122252 None:intergenic 35.0%
AGGTTACAAGACCAAACATA+AGG - chr4_1:65122249-65122268 None:intergenic 35.0%
! TTGGCTTGATTAGTCACAAA+GGG - chr4_1:65122334-65122353 None:intergenic 35.0%
!! TTTGGCTTGATTAGTCACAA+AGG - chr4_1:65122335-65122354 None:intergenic 35.0%
ACAATCTATGGTTGGAGATT+TGG - chr4_1:65122353-65122372 None:intergenic 35.0%
GGTCATCATGATCTTTGATA+AGG - chr4_1:65122426-65122445 None:intergenic 35.0%
!! TTTACTGATTTGGGTCATGT+GGG - chr4_1:65122762-65122781 None:intergenic 35.0%
!!! TTTTACTGATTTGGGTCATG+TGG - chr4_1:65122763-65122782 None:intergenic 35.0%
GATATATACAAACCTAGGTG+TGG - chr4_1:65122818-65122837 None:intergenic 35.0%
CCGTTGATATATACAAACCT+AGG - chr4_1:65122823-65122842 None:intergenic 35.0%
! AGCTTAGCATTGAAACTTCA+AGG + chr4_1:65123569-65123588 Msa0564460:intron 35.0%
ACAATTGCTCAAGACAAGAA+CGG + chr4_1:65123931-65123950 Msa0564460:CDS 35.0%
AGTTCAAACTACGAAGAAGT+AGG - chr4_1:65124025-65124044 None:intergenic 35.0%
TCAATAACACAGACAAGGAA+AGG - chr4_1:65124195-65124214 None:intergenic 35.0%
CATAAGGACTAGGAAAATCA+AGG - chr4_1:65122233-65122252 None:intergenic 35.0%
AGGTTACAAGACCAAACATA+AGG - chr4_1:65122249-65122268 None:intergenic 35.0%
! TTGGCTTGATTAGTCACAAA+GGG - chr4_1:65122334-65122353 None:intergenic 35.0%
!! TTTGGCTTGATTAGTCACAA+AGG - chr4_1:65122335-65122354 None:intergenic 35.0%
ACAATCTATGGTTGGAGATT+TGG - chr4_1:65122353-65122372 None:intergenic 35.0%
GGTCATCATGATCTTTGATA+AGG - chr4_1:65122426-65122445 None:intergenic 35.0%
!! TTTACTGATTTGGGTCATGT+GGG - chr4_1:65122762-65122781 None:intergenic 35.0%
!!! TTTTACTGATTTGGGTCATG+TGG - chr4_1:65122763-65122782 None:intergenic 35.0%
GATATATACAAACCTAGGTG+TGG - chr4_1:65122818-65122837 None:intergenic 35.0%
CCGTTGATATATACAAACCT+AGG - chr4_1:65122823-65122842 None:intergenic 35.0%
! AGCTTAGCATTGAAACTTCA+AGG + chr4_1:65123569-65123588 Msa0564460:intron 35.0%
ACAATTGCTCAAGACAAGAA+CGG + chr4_1:65123931-65123950 Msa0564460:CDS 35.0%
AGTTCAAACTACGAAGAAGT+AGG - chr4_1:65124025-65124044 None:intergenic 35.0%
TCAATAACACAGACAAGGAA+AGG - chr4_1:65124195-65124214 None:intergenic 35.0%
CAAGACCAAACATAAGGACT+AGG - chr4_1:65122243-65122262 None:intergenic 40.0%
AGCCAAAGTAAGGAGTTTGT+CGG - chr4_1:65122302-65122321 None:intergenic 40.0%
! CTTTGGTAACTTAAGGGCAA+GGG - chr4_1:65122476-65122495 None:intergenic 40.0%
GTAGGTGAGTTACAATCCTT+TGG - chr4_1:65122493-65122512 None:intergenic 40.0%
ACCTAAATCACATGAGGCTA+AGG + chr4_1:65122641-65122660 Msa0564460:CDS 40.0%
ACCTTAGCCTCATGTGATTT+AGG - chr4_1:65122645-65122664 None:intergenic 40.0%
! ACATGAGGCTAAGGTATTTG+AGG + chr4_1:65122650-65122669 Msa0564460:CDS 40.0%
TGAGGACTTCCAAAAATCCT+TGG + chr4_1:65122668-65122687 Msa0564460:CDS 40.0%
!!! GGTGGAAGTATTTTTCTCCA+AGG - chr4_1:65122688-65122707 None:intergenic 40.0%
AAATTTACCTGGAGGAACTG+CGG - chr4_1:65122709-65122728 None:intergenic 40.0%
TATACAAACCTAGGTGTGGA+TGG - chr4_1:65122814-65122833 None:intergenic 40.0%
CAACGACTCTAAATAGCCTT+AGG + chr4_1:65123726-65123745 Msa0564460:intron 40.0%
GAAGGATGGACCCTAATTAA+AGG - chr4_1:65124173-65124192 None:intergenic 40.0%
TAACACAGACAAGGAAAGGA+AGG - chr4_1:65124191-65124210 None:intergenic 40.0%
ACGACCTTGATGTCTAACTA+CGG - chr4_1:65124249-65124268 None:intergenic 40.0%
CAAGACCAAACATAAGGACT+AGG - chr4_1:65122243-65122262 None:intergenic 40.0%
AGCCAAAGTAAGGAGTTTGT+CGG - chr4_1:65122302-65122321 None:intergenic 40.0%
! CTTTGGTAACTTAAGGGCAA+GGG - chr4_1:65122476-65122495 None:intergenic 40.0%
GTAGGTGAGTTACAATCCTT+TGG - chr4_1:65122493-65122512 None:intergenic 40.0%
ACCTAAATCACATGAGGCTA+AGG + chr4_1:65122641-65122660 Msa0564460:CDS 40.0%
ACCTTAGCCTCATGTGATTT+AGG - chr4_1:65122645-65122664 None:intergenic 40.0%
! ACATGAGGCTAAGGTATTTG+AGG + chr4_1:65122650-65122669 Msa0564460:CDS 40.0%
TGAGGACTTCCAAAAATCCT+TGG + chr4_1:65122668-65122687 Msa0564460:CDS 40.0%
!!! GGTGGAAGTATTTTTCTCCA+AGG - chr4_1:65122688-65122707 None:intergenic 40.0%
AAATTTACCTGGAGGAACTG+CGG - chr4_1:65122709-65122728 None:intergenic 40.0%
TATACAAACCTAGGTGTGGA+TGG - chr4_1:65122814-65122833 None:intergenic 40.0%
CAACGACTCTAAATAGCCTT+AGG + chr4_1:65123726-65123745 Msa0564460:intron 40.0%
GAAGGATGGACCCTAATTAA+AGG - chr4_1:65124173-65124192 None:intergenic 40.0%
TAACACAGACAAGGAAAGGA+AGG - chr4_1:65124191-65124210 None:intergenic 40.0%
ACGACCTTGATGTCTAACTA+CGG - chr4_1:65124249-65124268 None:intergenic 40.0%
CACCGACAAACTCCTTACTT+TGG + chr4_1:65122297-65122316 Msa0564460:CDS 45.0%
GACAAACTCCTTACTTTGGC+TGG + chr4_1:65122301-65122320 Msa0564460:CDS 45.0%
GAAGACAACCAGCCAAAGTA+AGG - chr4_1:65122312-65122331 None:intergenic 45.0%
CCAGTGCAACAATCTATGGT+TGG - chr4_1:65122361-65122380 None:intergenic 45.0%
CCAACCATAGATTGTTGCAC+TGG + chr4_1:65122358-65122377 Msa0564460:CDS 45.0%
GACACCAGTGCAACAATCTA+TGG - chr4_1:65122365-65122384 None:intergenic 45.0%
! TTGTTGCACTGGTGTCAAAG+AGG + chr4_1:65122369-65122388 Msa0564460:CDS 45.0%
GATCATGATGACCCAAACCT+TGG + chr4_1:65122433-65122452 Msa0564460:CDS 45.0%
! CCTTTGGTAACTTAAGGGCA+AGG - chr4_1:65122477-65122496 None:intergenic 45.0%
CCTTGCCCTTAAGTTACCAA+AGG + chr4_1:65122474-65122493 Msa0564460:CDS 45.0%
TACAAGGAAGTCAAGAAGGG+TGG + chr4_1:65122783-65122802 Msa0564460:intron 45.0%
TGGAATTTCCATCCACACCT+AGG + chr4_1:65122803-65122822 Msa0564460:intron 45.0%
GTTAATCGAGCTGCACTTAC+TGG + chr4_1:65122989-65123008 Msa0564460:intron 45.0%
TGTGCTTGTTCCATTTCCAG+TGG - chr4_1:65123914-65123933 None:intergenic 45.0%
ATTGCTCAAGACAAGAACGG+TGG + chr4_1:65123934-65123953 Msa0564460:CDS 45.0%
ACAGACAAGGAAAGGAAGGA+TGG - chr4_1:65124187-65124206 None:intergenic 45.0%
! CTAACTACGGGACCCTTTTA+TGG - chr4_1:65124236-65124255 None:intergenic 45.0%
CGACCTTGATGTCTAACTAC+GGG - chr4_1:65124248-65124267 None:intergenic 45.0%
CACCGACAAACTCCTTACTT+TGG + chr4_1:65122297-65122316 Msa0564460:CDS 45.0%
GACAAACTCCTTACTTTGGC+TGG + chr4_1:65122301-65122320 Msa0564460:CDS 45.0%
GAAGACAACCAGCCAAAGTA+AGG - chr4_1:65122312-65122331 None:intergenic 45.0%
CCAGTGCAACAATCTATGGT+TGG - chr4_1:65122361-65122380 None:intergenic 45.0%
CCAACCATAGATTGTTGCAC+TGG + chr4_1:65122358-65122377 Msa0564460:CDS 45.0%
GACACCAGTGCAACAATCTA+TGG - chr4_1:65122365-65122384 None:intergenic 45.0%
! TTGTTGCACTGGTGTCAAAG+AGG + chr4_1:65122369-65122388 Msa0564460:CDS 45.0%
GATCATGATGACCCAAACCT+TGG + chr4_1:65122433-65122452 Msa0564460:CDS 45.0%
! CCTTTGGTAACTTAAGGGCA+AGG - chr4_1:65122477-65122496 None:intergenic 45.0%
CCTTGCCCTTAAGTTACCAA+AGG + chr4_1:65122474-65122493 Msa0564460:CDS 45.0%
TACAAGGAAGTCAAGAAGGG+TGG + chr4_1:65122783-65122802 Msa0564460:intron 45.0%
TGGAATTTCCATCCACACCT+AGG + chr4_1:65122803-65122822 Msa0564460:intron 45.0%
GTTAATCGAGCTGCACTTAC+TGG + chr4_1:65122989-65123008 Msa0564460:intron 45.0%
TGTGCTTGTTCCATTTCCAG+TGG - chr4_1:65123914-65123933 None:intergenic 45.0%
ATTGCTCAAGACAAGAACGG+TGG + chr4_1:65123934-65123953 Msa0564460:CDS 45.0%
ACAGACAAGGAAAGGAAGGA+TGG - chr4_1:65124187-65124206 None:intergenic 45.0%
! CTAACTACGGGACCCTTTTA+TGG - chr4_1:65124236-65124255 None:intergenic 45.0%
CGACCTTGATGTCTAACTAC+GGG - chr4_1:65124248-65124267 None:intergenic 45.0%
!!! TATTTTTTAATCTTAAATAA+AGG + chr4_1:65122736-65122755 Msa0564460:intron 5.0%
!!! CATATTTTATTAAAATATTT+AGG + chr4_1:65123308-65123327 Msa0564460:intron 5.0%
!!! TATTTTTTAATCTTAAATAA+AGG + chr4_1:65122736-65122755 Msa0564460:intron 5.0%
!!! CATATTTTATTAAAATATTT+AGG + chr4_1:65123308-65123327 Msa0564460:intron 5.0%
CGGTGCATTCTGCTTTGTCT+TGG - chr4_1:65122282-65122301 None:intergenic 50.0%
ATTGCAGCTAGTGGTACCAC+TGG + chr4_1:65123895-65123914 Msa0564460:intron 50.0%
GCTAGTGGTACCACTGGAAA+TGG + chr4_1:65123901-65123920 Msa0564460:intron 50.0%
TTGTGGAAAGAGGTGGCTCT+TGG + chr4_1:65123960-65123979 Msa0564460:CDS 50.0%
AAAGAGGTGGCTCTTGGTAG+AGG + chr4_1:65123966-65123985 Msa0564460:CDS 50.0%
!! CTCTTGGTAGAGGTGCTTTG+TGG + chr4_1:65123976-65123995 Msa0564460:CDS 50.0%
GGTCCCGTAGTTAGACATCA+AGG + chr4_1:65124242-65124261 Msa0564460:three_prime_UTR 50.0%
CGGTGCATTCTGCTTTGTCT+TGG - chr4_1:65122282-65122301 None:intergenic 50.0%
ATTGCAGCTAGTGGTACCAC+TGG + chr4_1:65123895-65123914 Msa0564460:intron 50.0%
GCTAGTGGTACCACTGGAAA+TGG + chr4_1:65123901-65123920 Msa0564460:intron 50.0%
TTGTGGAAAGAGGTGGCTCT+TGG + chr4_1:65123960-65123979 Msa0564460:CDS 50.0%
AAAGAGGTGGCTCTTGGTAG+AGG + chr4_1:65123966-65123985 Msa0564460:CDS 50.0%
!! CTCTTGGTAGAGGTGCTTTG+TGG + chr4_1:65123976-65123995 Msa0564460:CDS 50.0%
GGTCCCGTAGTTAGACATCA+AGG + chr4_1:65124242-65124261 Msa0564460:three_prime_UTR 50.0%
TTTACCTGGAGGAACTGCGG+TGG - chr4_1:65122706-65122725 None:intergenic 55.0%
GCTCAAGACAAGAACGGTGG+CGG + chr4_1:65123937-65123956 Msa0564460:CDS 55.0%
TTTACCTGGAGGAACTGCGG+TGG - chr4_1:65122706-65122725 None:intergenic 55.0%
GCTCAAGACAAGAACGGTGG+CGG + chr4_1:65123937-65123956 Msa0564460:CDS 55.0%
ACTTCCACCGCAGTTCCTCC+AGG + chr4_1:65122699-65122718 Msa0564460:CDS 60.0%
! GACAAGAACGGTGGCGGTTG+TGG + chr4_1:65123943-65123962 Msa0564460:CDS 60.0%
! ACGGTGGCGGTTGTGGAAAG+AGG + chr4_1:65123950-65123969 Msa0564460:CDS 60.0%
GTGGCGGTTGTGGAAAGAGG+TGG + chr4_1:65123953-65123972 Msa0564460:CDS 60.0%
ACTTCCACCGCAGTTCCTCC+AGG + chr4_1:65122699-65122718 Msa0564460:CDS 60.0%
! GACAAGAACGGTGGCGGTTG+TGG + chr4_1:65123943-65123962 Msa0564460:CDS 60.0%
! ACGGTGGCGGTTGTGGAAAG+AGG + chr4_1:65123950-65123969 Msa0564460:CDS 60.0%
GTGGCGGTTGTGGAAAGAGG+TGG + chr4_1:65123953-65123972 Msa0564460:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 65122080 65124289 65122080 ID=Msa0564460;Name=Msa0564460
chr4_1 mRNA 65122080 65124289 65122080 ID=Msa0564460-mRNA-1;Parent=Msa0564460;Name=Msa0564460-mRNA-1;_AED=0.06;_eAED=0.06;_QI=110|1|1|1|1|1|3|248|192
chr4_1 exon 65122080 65122532 65122080 ID=Msa0564460-mRNA-1:exon:20923;Parent=Msa0564460-mRNA-1
chr4_1 exon 65122625 65122720 65122625 ID=Msa0564460-mRNA-1:exon:20924;Parent=Msa0564460-mRNA-1
chr4_1 exon 65123902 65124289 65123902 ID=Msa0564460-mRNA-1:exon:20925;Parent=Msa0564460-mRNA-1
chr4_1 five_prime_UTR 65122080 65122189 65122080 ID=Msa0564460-mRNA-1:five_prime_utr;Parent=Msa0564460-mRNA-1
chr4_1 CDS 65122190 65122532 65122190 ID=Msa0564460-mRNA-1:cds;Parent=Msa0564460-mRNA-1
chr4_1 CDS 65122625 65122720 65122625 ID=Msa0564460-mRNA-1:cds;Parent=Msa0564460-mRNA-1
chr4_1 CDS 65123902 65124041 65123902 ID=Msa0564460-mRNA-1:cds;Parent=Msa0564460-mRNA-1
chr4_1 three_prime_UTR 65124042 65124289 65124042 ID=Msa0564460-mRNA-1:three_prime_utr;Parent=Msa0564460-mRNA-1
Gene Sequence

>Msa0564460

ATGAGTTGCAAAAGTACAACAAATCTATCACTCTTCTCTACCTTGATTTTCCTAGTCCTTATGTTTGGTCTTGTAACCTCAGATATTAACCAAGACAAAGCAGAATGCACCGACAAACTCCTTACTTTGGCTGGTTGTCTTCCCTTTGTGACTAATCAAGCCAAATCTCCAACCATAGATTGTTGCACTGGTGTCAAAGAGGTTGTTGATAAGAGTAAGAGATGTTTATGTATCCTTATCAAAGATCATGATGACCCAAACCTTGGTTTAACTATAAATGTTACCCTTGCCCTTAAGTTACCAAAGGATTGTAACTCACCTACCAATATAACTCAATGTATTGATATTTTGCATTTGAAACCTAAATCACATGAGGCTAAGGTATTTGAGGACTTCCAAAAATCCTTGGAGAAAAATACTTCCACCGCAGTTCCTCCAGCTAGTGGTACCACTGGAAATGGAACAAGCACAATTGCTCAAGACAAGAACGGTGGCGGTTGTGGAAAGAGGTGGCTCTTGGTAGAGGTGCTTTGTGGCATTTTACCATATGTTTTAGTTTCCTACTTCTTCGTAGTTTGA
Protein sequence

>Msa0564460

MSCKSTTNLSLFSTLIFLVLMFGLVTSDINQDKAECTDKLLTLAGCLPFVTNQAKSPTIDCCTGVKEVVDKSKRCLCILIKDHDDPNLGLTINVTLALKLPKDCNSPTNITQCIDILHLKPKSHEAKVFEDFQKSLEKNTSTAVPPASGTTGNGTSTIAQDKNGGGCGKRWLLVEVLCGILPYVLVSYFFVV*