AlfalfaGEDB | Msa0656730

M. sativa cultivar zhongmu-4 / Msa0656730

KEGG: -
GO: -
PFAM: PF14368.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0656730	XP_003629697.1	95.361	194	7	1	1	192	1	194	1.24e-127	367

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0656730	sp\|O64865\|LTG14_ARATH	36.275	204	110	3	6	192	3	203	4.54e-41	140

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0039490	Msa0656730	0.804653	1.947801e-49	-8.615850e-47
Msa0043940	Msa0656730	0.826195	3.098601e-54	-8.615850e-47
Msa0073820	Msa0656730	0.803562	3.284841e-49	-8.615850e-47
Msa0078090	Msa0656730	0.805610	1.228306e-49	-8.615850e-47
Msa0089210	Msa0656730	0.811530	6.686277e-51	-8.615850e-47
Msa0092710	Msa0656730	0.824828	6.533648e-54	-8.615850e-47
Msa0092730	Msa0656730	0.810525	1.103972e-50	-8.615850e-47
Msa0604700	Msa0656730	0.805369	1.380029e-49	-8.615850e-47
Msa0607480	Msa0656730	0.804632	1.968215e-49	-8.615850e-47
Msa0617760	Msa0656730	0.922750	6.548460e-89	-8.615850e-47
Msa0647750	Msa0656730	0.808099	3.657590e-50	-8.615850e-47
Msa0656730	Msa0667970	0.834977	2.193159e-56	-8.615850e-47
Msa0656730	Msa0700740	0.914798	1.255635e-84	-8.615850e-47
Msa0656730	Msa0703760	0.800079	1.705160e-48	-8.615850e-47
Msa0656730	Msa0720270	0.817348	3.455436e-52	-8.615850e-47
Msa0656730	Msa0760890	0.803479	3.418323e-49	-8.615850e-47
Msa0656730	Msa0761790	0.822911	1.838295e-53	-8.615850e-47
Msa0656730	Msa0770430	0.805930	1.052485e-49	-8.615850e-47
Msa0656730	Msa0780140	0.809022	2.323054e-50	-8.615850e-47
Msa0656730	Msa0802210	0.817515	3.168322e-52	-8.615850e-47
Msa0656730	Msa0803130	0.800138	1.658940e-48	-8.615850e-47
Msa0656730	Msa0819370	0.801362	9.332950e-49	-8.615850e-47
Msa0656730	Msa0882700	0.801523	8.648172e-49	-8.615850e-47
Msa0656730	Msa0931480	0.801820	7.516415e-49	-8.615850e-47
Msa0656730	Msa1027790	0.831452	1.655836e-55	-8.615850e-47
Msa0656730	Msa1030870	0.814231	1.711367e-51	-8.615850e-47
Msa0656730	Msa1080620	0.830896	2.267918e-55	-8.615850e-47
Msa0656730	Msa1080690	0.833130	6.362280e-56	-8.615850e-47
Msa0656730	Msa1106560	0.803974	2.698010e-49	-8.615850e-47
Msa0656730	Msa1106580	0.808953	2.403667e-50	-8.615850e-47
Msa0656730	Msa1112610	0.827663	1.381580e-54	-8.615850e-47
Msa0656730	Msa1126310	0.809046	2.295633e-50	-8.615850e-47
Msa0656730	Msa1127510	0.801240	9.884654e-49	-8.615850e-47
Msa0656730	Msa1134400	0.811820	5.783001e-51	-8.615850e-47
Msa0656730	Msa1144950	0.805953	1.040671e-49	-8.615850e-47
Msa0656730	Msa1167160	0.814728	1.329200e-51	-8.615850e-47
Msa0656730	Msa1183390	0.804558	2.039076e-49	-8.615850e-47
Msa0656730	Msa1190910	0.802709	4.932535e-49	-8.615850e-47
Msa0656730	Msa1197110	0.822494	2.298792e-53	-8.615850e-47
Msa0656730	Msa1219250	0.812783	3.563830e-51	-8.615850e-47
Msa0656730	Msa1250670	0.814526	1.473066e-51	-8.615850e-47
Msa0656730	Msa1271510	0.809986	1.442811e-50	-8.615850e-47
Msa0656730	Msa1302150	0.810458	1.141527e-50	-8.615850e-47
Msa0656730	Msa1309360	0.801278	9.710176e-49	-8.615850e-47
Msa0656730	Msa1412520	0.824403	8.226084e-54	-8.615850e-47
Msa0656730	Msa1439120	0.832133	1.124999e-55	-8.615850e-47
Msa0656730	Msa1440220	0.814086	1.842835e-51	-8.615850e-47
Msa0656730	Msa1455660	0.830857	2.318202e-55	-8.615850e-47
Msa0134040	Msa0656730	0.807017	6.208016e-50	-8.615850e-47
Msa0135980	Msa0656730	0.805502	1.294151e-49	-8.615850e-47
Msa0137600	Msa0656730	0.812312	4.516967e-51	-8.615850e-47
Msa0150210	Msa0656730	0.802972	4.353336e-49	-8.615850e-47
Msa0157020	Msa0656730	0.802828	4.660441e-49	-8.615850e-47
Msa0171930	Msa0656730	0.809291	2.035168e-50	-8.615850e-47
Msa0181090	Msa0656730	0.832516	9.042797e-56	-8.615850e-47
Msa0182640	Msa0656730	0.806730	7.136693e-50	-8.615850e-47
Msa0217470	Msa0656730	0.802949	4.400568e-49	-8.615850e-47
Msa0367370	Msa0656730	0.840004	1.128565e-57	-8.615850e-47
Msa0379390	Msa0656730	0.801449	8.956724e-49	-8.615850e-47
Msa0411560	Msa0656730	0.812746	3.630646e-51	-8.615850e-47
Msa0450460	Msa0656730	0.804496	2.101169e-49	-8.615850e-47
Msa0473530	Msa0656730	0.806572	7.709295e-50	-8.615850e-47
Msa0296610	Msa0656730	0.818006	2.454784e-52	-8.615850e-47
Msa0323990	Msa0656730	0.803482	3.413713e-49	-8.615850e-47
Msa0334980	Msa0656730	0.831066	2.060617e-55	-8.615850e-47
Msa0532840	Msa0656730	0.835805	1.355250e-56	-8.615850e-47
Msa0533010	Msa0656730	0.828888	6.999939e-55	-8.615850e-47
Msa0561510	Msa0656730	0.823528	1.319408e-53	-8.615850e-47
Msa0564460	Msa0656730	0.915113	8.657320e-85	-8.615850e-47
Msa0587900	Msa0656730	0.809838	1.552089e-50	-8.615850e-47
Msa0589290	Msa0656730	0.801482	8.819217e-49	-8.615850e-47
Msa0594390	Msa0656730	0.800700	1.274348e-48	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0656730	MtrunA17_Chr8g0378461	95.361	194	7	1	1	192	1	194	1.14e-131	367
Msa0656730	MtrunA17_Chr3g0121381	51.765	170	80	1	23	192	18	185	4.25e-57	178
Msa0656730	MtrunA17_Chr8g0356191	38.728	173	104	2	15	185	13	185	4.41e-39	132
Msa0656730	MtrunA17_Chr7g0219391	45.631	103	52	1	15	113	10	112	4.40e-27	99.8
Msa0656730	MtrunA17_Chr1g0205191	40.789	76	42	1	33	108	25	97	2.76e-12	60.8
Msa0656730	MtrunA17_Chr1g0205181	37.736	106	58	3	9	113	6	104	3.97e-12	61.2
Msa0656730	MtrunA17_Chr5g0395001	27.778	144	94	4	15	154	12	149	3.17e-11	59.3
Msa0656730	MtrunA17_Chr5g0394991	34.862	109	64	4	10	113	6	112	4.72e-11	59.7
Msa0656730	MtrunA17_Chr1g0205201	39.024	82	48	2	33	113	25	105	6.20e-11	58.2
Msa0656730	MtrunA17_Chr5g0429901	32.653	147	79	6	14	149	6	143	9.30e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0656730	AT2G44300.1	36.275	204	110	3	6	192	3	203	4.62e-42	140
Msa0656730	AT2G44290.1	35.567	194	107	2	14	192	14	204	1.19e-41	139
Msa0656730	AT1G55260.1	41.622	185	96	5	10	190	50	226	1.40e-41	140
Msa0656730	AT1G55260.2	41.209	182	98	4	10	190	50	223	1.80e-41	139
Msa0656730	AT3G58550.1	39.333	150	88	2	8	154	10	159	2.93e-33	117
Msa0656730	AT3G22600.1	32.967	91	60	1	15	105	7	96	6.32e-14	67.0
Msa0656730	AT2G48130.2	35.000	100	63	2	15	113	9	107	1.78e-13	65.9
Msa0656730	AT2G48130.6	35.000	100	63	2	15	113	9	107	1.78e-13	65.9
Msa0656730	AT2G48130.4	35.000	100	63	2	15	113	9	107	1.78e-13	65.9
Msa0656730	AT2G48130.5	35.000	100	63	2	15	113	9	107	1.78e-13	65.9
Msa0656730	AT2G48130.1	35.000	100	63	2	15	113	9	107	1.78e-13	65.9
Msa0656730	AT2G48130.3	35.000	100	63	2	15	113	9	107	1.78e-13	65.9
Msa0656730	AT1G73890.1	36.471	85	54	0	29	113	23	107	4.13e-13	65.1
Msa0656730	AT4G14815.1	35.106	94	57	2	15	108	8	97	9.64e-12	60.8
Msa0656730	AT1G03103.1	32.967	91	57	1	15	105	8	94	3.90e-11	59.3
Msa0656730	AT1G05450.2	29.448	163	103	4	13	169	10	166	6.67e-11	59.3

Find 69 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAACTAATTGATCCATAAA+AGG	0.152918	4_3:-66089149	Msa0656730:three_prime_UTR
TACATGAAAATCTATCATTT+TGG	0.181442	4_3:-66088907	Msa0656730:three_prime_UTR
ACCTTAGCCTCATGTGATTT+AGG	0.238066	4_3:+66091149	None:intergenic
ATTGATAAAGACATCAAATT+TGG	0.249075	4_3:-66089018	Msa0656730:three_prime_UTR
ACAATCTATGGTTGGAGATT+TGG	0.249161	4_3:+66091441	None:intergenic
CTATTTCATCATCTTCAAAA+TGG	0.259507	4_3:-66091620	Msa0656730:exon
TACAATCCTTTGGTAACTTA+AGG	0.268613	4_3:+66091311	None:intergenic
GACACCAGTGCAACAATCTA+TGG	0.281431	4_3:+66091429	None:intergenic
ACCAATACATTGAGTTATAT+TGG	0.304514	4_3:+66091279	None:intergenic
TTTATAGTTAAACCAAGGTT+TGG	0.345145	4_3:+66091346	None:intergenic
GAAGGATGGACCCTAATTAA+AGG	0.348230	4_3:+66089199	None:intergenic
GGTCATCATGATCTTTGATA+AGG	0.380512	4_3:+66091368	None:intergenic
ACAATCCTTTGGTAACTTAA+GGG	0.380953	4_3:+66091312	None:intergenic
GACAAGAACGGTGGCGGTTG+TGG	0.396527	4_3:-66089428	Msa0656730:CDS
TATATACCTACCTGATTGGT+TGG	0.407980	4_3:-66089041	Msa0656730:three_prime_UTR
CGGTGCATTCTGCTTTGTCT+TGG	0.411232	4_3:+66091512	None:intergenic
GACAAACTCCTTACTTTGGC+TGG	0.412680	4_3:-66091490	Msa0656730:CDS
GTAGGTGAGTTACAATCCTT+TGG	0.416024	4_3:+66091301	None:intergenic
TATTTCATCATCTTCAAAAT+GGG	0.420364	4_3:-66091619	Msa0656730:exon
TTATAGTTAAACCAAGGTTT+GGG	0.422627	4_3:+66091347	None:intergenic
TATATATTATTGCTATATAT+CGG	0.427844	4_3:-66089292	Msa0656730:three_prime_UTR
AAATGTCAACATTTAATAAG+AGG	0.437356	4_3:-66089316	Msa0656730:three_prime_UTR
CTCTTGGTAGAGGTGCTTTG+TGG	0.443821	4_3:-66089395	Msa0656730:CDS
CAACTAATTGATCCATAAAA+GGG	0.451943	4_3:-66089148	Msa0656730:three_prime_UTR
ACCAATATAACTCAATGTAT+TGG	0.457228	4_3:-66091280	Msa0656730:intron
CACCGACAAACTCCTTACTT+TGG	0.466376	4_3:-66091494	Msa0656730:CDS
TAGTTATATACCTACCTGAT+TGG	0.469652	4_3:-66089045	Msa0656730:three_prime_UTR
GAAGACAACCAGCCAAAGTA+AGG	0.470397	4_3:+66091482	None:intergenic
CTTTATCAATCCAACCAATC+AGG	0.471522	4_3:+66089031	None:intergenic
ACATGAGGCTAAGGTATTTG+AGG	0.480429	4_3:-66091141	Msa0656730:CDS
ACCTAAATCACATGAGGCTA+AGG	0.495814	4_3:-66091150	Msa0656730:CDS
TTTGTCTTGGTTAATATCTG+AGG	0.495906	4_3:+66091525	None:intergenic
AAGTAGGAAACTAAAACAAA+TGG	0.516446	4_3:+66089365	None:intergenic
AAAGAGGTGGCTCTTGGTAG+AGG	0.525495	4_3:-66089405	Msa0656730:CDS
ACTTCCACCGCAGTTCCTCC+AGG	0.529242	4_3:-66091092	Msa0656730:intron
CATAAGGACTAGGAAAATCA+AGG	0.531221	4_3:+66091561	None:intergenic
GATCATGATGACCCAAACCT+TGG	0.532203	4_3:-66091358	Msa0656730:CDS
TTGGCTTGATTAGTCACAAA+GGG	0.535509	4_3:+66091460	None:intergenic
TTGTGGAAAGAGGTGGCTCT+TGG	0.535765	4_3:-66089411	Msa0656730:CDS
AATTCAAACTACGAAGAAGT+AGG	0.544914	4_3:+66089349	None:intergenic
CTTTGGTAACTTAAGGGCAA+GGG	0.546332	4_3:+66091318	None:intergenic
CCTTTGGTAACTTAAGGGCA+AGG	0.546657	4_3:+66091317	None:intergenic
AGGTTACAAGACCAAACATA+AGG	0.547469	4_3:+66091545	None:intergenic
CCAACCATAGATTGTTGCAC+TGG	0.548725	4_3:-66091433	Msa0656730:CDS
TGTGCTTGTTTCATTTCCAG+TGG	0.551525	4_3:+66089460	None:intergenic
CGACCTTGATGTCTAACTAC+GGG	0.556444	4_3:+66089124	None:intergenic
ACGACCTTGATGTCTAACTA+CGG	0.557088	4_3:+66089123	None:intergenic
ATTGCAGCTAGTGGTACCAC+TGG	0.560811	4_3:-66089476	Msa0656730:intron
CCTTGCCCTTAAGTTACCAA+AGG	0.561963	4_3:-66091317	Msa0656730:CDS
ACGGTGGCGGTTGTGGAAAG+AGG	0.565669	4_3:-66089421	Msa0656730:CDS
CAAGACCAAACATAAGGACT+AGG	0.566810	4_3:+66091551	None:intergenic
AGCCAAAGTAAGGAGTTTGT+CGG	0.567852	4_3:+66091492	None:intergenic
TAACACAGACAAGGAAAGGA+AGG	0.575654	4_3:+66089181	None:intergenic
TCAATAACACAGACAAGGAA+AGG	0.576335	4_3:+66089177	None:intergenic
ATACATTGAGTTATATTGGT+AGG	0.586657	4_3:+66091283	None:intergenic
GCTCAAGACAAGAACGGTGG+CGG	0.587172	4_3:-66089434	Msa0656730:CDS
GGTCCCGTAGTTAGACATCA+AGG	0.593276	4_3:-66089127	Msa0656730:three_prime_UTR
ACAGACAAGGAAAGGAAGGA+TGG	0.594898	4_3:+66089185	None:intergenic
CCAGTGCAACAATCTATGGT+TGG	0.604106	4_3:+66091433	None:intergenic
GTGGCGGTTGTGGAAAGAGG+TGG	0.614207	4_3:-66089418	Msa0656730:CDS
TAACATTTATAGTTAAACCA+AGG	0.621795	4_3:+66091341	None:intergenic
TTTGGCTTGATTAGTCACAA+AGG	0.627914	4_3:+66091459	None:intergenic
TTTGAAACCTAAATCACATG+AGG	0.643872	4_3:-66091156	Msa0656730:CDS
TTTACCTGGAGGAACTGCGG+TGG	0.660595	4_3:+66091088	None:intergenic
AAATTTACCTGGAGGAACTG+CGG	0.666666	4_3:+66091085	None:intergenic
ACAATTGCTCAAGACAAGAA+CGG	0.667135	4_3:-66089440	Msa0656730:CDS
TTGTTGCACTGGTGTCAAAG+AGG	0.679195	4_3:-66091422	Msa0656730:CDS
ATCAATCCAACCAATCAGGT+AGG	0.683610	4_3:+66089035	None:intergenic
ATTGCTCAAGACAAGAACGG+TGG	0.760005	4_3:-66089437	Msa0656730:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATATTTTATTAAAATATTTA+GGG	-	chr4_3:66090368-66090387	Msa0656730:intron	0.0%
!!!	TTCTTTTTATTTATGTTATA+AGG	+	chr4_3:66088942-66088961	None:intergenic	10.0%
!!	AAGAAAAATGTATAAATAAA+GGG	+	chr4_3:66090058-66090077	None:intergenic	10.0%
!!	TAAGAAAAATGTATAAATAA+AGG	+	chr4_3:66090059-66090078	None:intergenic	10.0%
!!	AATTAAATATCAAATTTAAC+AGG	+	chr4_3:66090494-66090513	None:intergenic	10.0%
!!	TATATATTATTGCTATATAT+CGG	-	chr4_3:66091310-66091329	Msa0656730:CDS	10.0%
!!	ATAAATAAAAAGAAAACAAG+AGG	-	chr4_3:66088948-66088967	Msa0656730:three_prime_UTR	15.0%
!!	AATAGTAAATAAAATTTACC+TGG	+	chr4_3:66089531-66089550	None:intergenic	15.0%
!!	AGAAAAATGTATAAATAAAG+GGG	+	chr4_3:66090057-66090076	None:intergenic	15.0%
!!	GTTTAAAAATTAATGAAGTA+TGG	-	chr4_3:66090304-66090323	Msa0656730:intron	15.0%
!!!	TTTTTAAACTTTTAATGCAA+TGG	-	chr4_3:66090669-66090688	Msa0656730:intron	15.0%
!!	TTCTATTTGTTTGTTTATAT+GGG	+	chr4_3:66090769-66090788	None:intergenic	15.0%
!!	TTTCTATTTGTTTGTTTATA+TGG	+	chr4_3:66090770-66090789	None:intergenic	15.0%
!!!	ACAAATAAAAAGTATCATTT+GGG	+	chr4_3:66090937-66090956	None:intergenic	15.0%
!!!	GATTTTGATAAAATTATAGT+TGG	-	chr4_3:66091615-66091634	Msa0656730:exon	15.0%
!!!	TTATCTTTTTTTTAAGAAGT+TGG	-	chr4_3:66091641-66091660	Msa0656730:five_prime_UTR	15.0%
!!	TATTTCATCATCTTCAAAAT+GGG	-	chr4_3:66088983-66089002	Msa0656730:three_prime_UTR	20.0%
!!	TAACATTTATAGTTAAACCA+AGG	+	chr4_3:66089264-66089283	None:intergenic	20.0%
!!!	TGAAACAAATATCAAACTTT+TGG	+	chr4_3:66089415-66089434	None:intergenic	20.0%
!!	AATATAGAAACAAAAGACTA+AGG	-	chr4_3:66089570-66089589	Msa0656730:intron	20.0%
!!!	TTTTCTATCACATAATAAAG+TGG	-	chr4_3:66089713-66089732	Msa0656730:intron	20.0%
!!	TTATTAAAGATCATATAGTG+TGG	+	chr4_3:66089850-66089869	None:intergenic	20.0%
!!!	GACACTTTTTTTTAGAAAAT+GGG	+	chr4_3:66089924-66089943	None:intergenic	20.0%
!!	AAAGTGGAATATATTGTATT+GGG	+	chr4_3:66090034-66090053	None:intergenic	20.0%
!!	GTTTGAAGAGAATAAAAATT+AGG	-	chr4_3:66090460-66090479	Msa0656730:intron	20.0%
!!!	TGATTTAAAATTTCCACTTT+TGG	+	chr4_3:66090639-66090658	None:intergenic	20.0%
!!!	CAATTTCTTTTTCTAAAGTT+AGG	+	chr4_3:66090849-66090868	None:intergenic	20.0%
!!!	GACAAATAAAAAGTATCATT+TGG	+	chr4_3:66090938-66090957	None:intergenic	20.0%
!!!	TATTTTTGGATAAAAGTGAT+TGG	-	chr4_3:66091087-66091106	Msa0656730:intron	20.0%
!!	AAATGTCAACATTTAATAAG+AGG	-	chr4_3:66091286-66091305	Msa0656730:CDS	20.0%
!!!	ATTGTCTTTTTCCTTTAATT+AGG	-	chr4_3:66091392-66091411	Msa0656730:CDS	20.0%
!!!	TTGTCTTTTTCCTTTAATTA+GGG	-	chr4_3:66091393-66091412	Msa0656730:CDS	20.0%
!!!	ATATTTTCGTGATGATAAAT+AGG	-	chr4_3:66091515-66091534	Msa0656730:CDS	20.0%
!!	ATTGATAAAGACATCAAATT+TGG	-	chr4_3:66091584-66091603	Msa0656730:CDS	20.0%
!!!	TACATGAAAATCTATCATTT+TGG	-	chr4_3:66091695-66091714	Msa0656730:five_prime_UTR	20.0%
!	CTATTTCATCATCTTCAAAA+TGG	-	chr4_3:66088982-66089001	Msa0656730:three_prime_UTR	25.0%
!	TTATAGTTAAACCAAGGTTT+GGG	+	chr4_3:66089258-66089277	None:intergenic	25.0%
!	TTTATAGTTAAACCAAGGTT+TGG	+	chr4_3:66089259-66089278	None:intergenic	25.0%
!	ATACATTGAGTTATATTGGT+AGG	+	chr4_3:66089322-66089341	None:intergenic	25.0%
!	ACCAATATAACTCAATGTAT+TGG	-	chr4_3:66089322-66089341	Msa0656730:three_prime_UTR	25.0%
!	ACCAATACATTGAGTTATAT+TGG	+	chr4_3:66089326-66089345	None:intergenic	25.0%
!!	TATTTTTCTCCAAGGATTTT+TGG	+	chr4_3:66089491-66089510	None:intergenic	25.0%
!	AGTAAATAAAATTTACCTGG+AGG	+	chr4_3:66089528-66089547	None:intergenic	25.0%
!!!	TCCTTGTATTTTACTGATTT+GGG	+	chr4_3:66089605-66089624	None:intergenic	25.0%
!!!	TTCCTTGTATTTTACTGATT+TGG	+	chr4_3:66089606-66089625	None:intergenic	25.0%
!!!	GGACACTTTTTTTTAGAAAA+TGG	+	chr4_3:66089925-66089944	None:intergenic	25.0%
!!!	TGAAATGACTCAGTTATTTT+GGG	+	chr4_3:66089946-66089965	None:intergenic	25.0%
!!	TTGAAATGACTCAGTTATTT+TGG	+	chr4_3:66089947-66089966	None:intergenic	25.0%
!	GAAAGTGGAATATATTGTAT+TGG	+	chr4_3:66090035-66090054	None:intergenic	25.0%
!!!	CACTATTTTGATTGACATTT+GGG	+	chr4_3:66090141-66090160	None:intergenic	25.0%
!!!	TCACTATTTTGATTGACATT+TGG	+	chr4_3:66090142-66090161	None:intergenic	25.0%
!	AGAAAAAGAAATTGATGTAG+AGG	-	chr4_3:66090855-66090874	Msa0656730:intron	25.0%
!!	ATCTCATTTGATTTCATTCT+AGG	-	chr4_3:66091026-66091045	Msa0656730:intron	25.0%
!!!	TGATCTTGCTTGTTTATTTT+TGG	-	chr4_3:66091073-66091092	Msa0656730:intron	25.0%
!	AAGTAGGAAACTAAAACAAA+TGG	+	chr4_3:66091240-66091259	None:intergenic	25.0%
!	AAAAATCAATAACACAGACA+AGG	+	chr4_3:66091433-66091452	None:intergenic	25.0%
!	CAACTAATTGATCCATAAAA+GGG	-	chr4_3:66091454-66091473	Msa0656730:CDS	25.0%
!	AACTACGTATAAAAATAGAG+AGG	+	chr4_3:66091542-66091561	None:intergenic	25.0%
!	AAAAAGAAAACAAGAGGTGT+TGG	-	chr4_3:66088954-66088973	Msa0656730:three_prime_UTR	30.0%
!	ATTTTCCTAGTCCTTATGTT+TGG	-	chr4_3:66089046-66089065	Msa0656730:three_prime_UTR	30.0%
	TTTGTCTTGGTTAATATCTG+AGG	+	chr4_3:66089080-66089099	None:intergenic	30.0%
!	ACAATCCTTTGGTAACTTAA+GGG	+	chr4_3:66089293-66089312	None:intergenic	30.0%
!	TACAATCCTTTGGTAACTTA+AGG	+	chr4_3:66089294-66089313	None:intergenic	30.0%
!	TTTGAAACCTAAATCACATG+AGG	-	chr4_3:66089446-66089465	Msa0656730:CDS	30.0%
	ACCCAAATCAGTAAAATACA+AGG	-	chr4_3:66089601-66089620	Msa0656730:intron	30.0%
	AAAATACAAGGAAGTCAAGA+AGG	-	chr4_3:66089613-66089632	Msa0656730:intron	30.0%
	AAATACAAGGAAGTCAAGAA+GGG	-	chr4_3:66089614-66089633	Msa0656730:intron	30.0%
	CCTAGGTTTGTATATATCAA+CGG	-	chr4_3:66089654-66089673	Msa0656730:intron	30.0%
	CTAGGTTTGTATATATCAAC+GGG	-	chr4_3:66089655-66089674	Msa0656730:intron	30.0%
	TGTATAAATAAAGGGGAAAG+TGG	+	chr4_3:66090050-66090069	None:intergenic	30.0%
	AAGACTCGAAAAACAAATTG+AGG	-	chr4_3:66090909-66090928	Msa0656730:intron	30.0%
	AGACTCGAAAAACAAATTGA+GGG	-	chr4_3:66090910-66090929	Msa0656730:intron	30.0%
	TAACAAAACATGAAGACCTA+AGG	+	chr4_3:66090976-66090995	None:intergenic	30.0%
	CTTAATAGAGATTAGAGTGA+AGG	+	chr4_3:66091005-66091024	None:intergenic	30.0%
	AATTCATTAATTGCAGCTAG+TGG	-	chr4_3:66091117-66091136	Msa0656730:CDS	30.0%
	AATTCAAACTACGAAGAAGT+AGG	+	chr4_3:66091256-66091275	None:intergenic	30.0%
!	CCTTTTATGGATCAATTAGT+TGG	+	chr4_3:66091456-66091475	None:intergenic	30.0%
	CCAACTAATTGATCCATAAA+AGG	-	chr4_3:66091453-66091472	Msa0656730:CDS	30.0%
	TAGTTATATACCTACCTGAT+TGG	-	chr4_3:66091557-66091576	Msa0656730:CDS	30.0%
	CATAAGGACTAGGAAAATCA+AGG	+	chr4_3:66089044-66089063	None:intergenic	35.0%
	AGGTTACAAGACCAAACATA+AGG	+	chr4_3:66089060-66089079	None:intergenic	35.0%
!	TTGGCTTGATTAGTCACAAA+GGG	+	chr4_3:66089145-66089164	None:intergenic	35.0%
!!	TTTGGCTTGATTAGTCACAA+AGG	+	chr4_3:66089146-66089165	None:intergenic	35.0%
	ACAATCTATGGTTGGAGATT+TGG	+	chr4_3:66089164-66089183	None:intergenic	35.0%
	GGTCATCATGATCTTTGATA+AGG	+	chr4_3:66089237-66089256	None:intergenic	35.0%
!!	TTTACTGATTTGGGTCATGT+GGG	+	chr4_3:66089596-66089615	None:intergenic	35.0%
!!!	TTTTACTGATTTGGGTCATG+TGG	+	chr4_3:66089597-66089616	None:intergenic	35.0%
	CCGTTGATATATACAAACCT+AGG	+	chr4_3:66089657-66089676	None:intergenic	35.0%
	AGAAAATGGGTCAGATATTC+CGG	+	chr4_3:66089911-66089930	None:intergenic	35.0%
	ACAATTGCTCAAGACAAGAA+CGG	-	chr4_3:66091162-66091181	Msa0656730:CDS	35.0%
	TCAATAACACAGACAAGGAA+AGG	+	chr4_3:66091428-66091447	None:intergenic	35.0%
	TATATACCTACCTGATTGGT+TGG	-	chr4_3:66091561-66091580	Msa0656730:CDS	35.0%
	CTTTATCAATCCAACCAATC+AGG	+	chr4_3:66091574-66091593	None:intergenic	35.0%
	CAAGACCAAACATAAGGACT+AGG	+	chr4_3:66089054-66089073	None:intergenic	40.0%
	AGCCAAAGTAAGGAGTTTGT+CGG	+	chr4_3:66089113-66089132	None:intergenic	40.0%
!	CTTTGGTAACTTAAGGGCAA+GGG	+	chr4_3:66089287-66089306	None:intergenic	40.0%
	GTAGGTGAGTTACAATCCTT+TGG	+	chr4_3:66089304-66089323	None:intergenic	40.0%
	ACCTAAATCACATGAGGCTA+AGG	-	chr4_3:66089452-66089471	Msa0656730:CDS	40.0%
	ACCTTAGCCTCATGTGATTT+AGG	+	chr4_3:66089456-66089475	None:intergenic	40.0%
!	ACATGAGGCTAAGGTATTTG+AGG	-	chr4_3:66089461-66089480	Msa0656730:CDS	40.0%
	TGAGGACTTCCAAAAATCCT+TGG	-	chr4_3:66089479-66089498	Msa0656730:intron	40.0%
!!!	GGTGGAAGTATTTTTCTCCA+AGG	+	chr4_3:66089499-66089518	None:intergenic	40.0%
	AAATTTACCTGGAGGAACTG+CGG	+	chr4_3:66089520-66089539	None:intergenic	40.0%
	GATATATACAAACCTAGGCG+TGG	+	chr4_3:66089652-66089671	None:intergenic	40.0%
	CATATAGTGTGGTCCAGTAA+GGG	+	chr4_3:66089839-66089858	None:intergenic	40.0%
	TCATATAGTGTGGTCCAGTA+AGG	+	chr4_3:66089840-66089859	None:intergenic	40.0%
	ACTTAATACTCCCTTCGTTC+CGG	-	chr4_3:66089889-66089908	Msa0656730:intron	40.0%
	ATCTGTGTGAAATGAGCCAT+TGG	-	chr4_3:66090079-66090098	Msa0656730:intron	40.0%
!!	AGCCATTGGCTCAGATAAAT+TGG	-	chr4_3:66090093-66090112	Msa0656730:intron	40.0%
!!	GCCATTGGCTCAGATAAATT+GGG	-	chr4_3:66090094-66090113	Msa0656730:intron	40.0%
	TCCCAATTTATCTGAGCCAA+TGG	+	chr4_3:66090098-66090117	None:intergenic	40.0%
	TTGGCTCAGATAAATTGGGA+CGG	-	chr4_3:66090098-66090117	Msa0656730:intron	40.0%
!	CTTTTGGATTCCTTAACCAG+TGG	+	chr4_3:66090623-66090642	None:intergenic	40.0%
	CTGGTTAAGGAATCCAAAAG+TGG	-	chr4_3:66090623-66090642	Msa0656730:intron	40.0%
!!!	GGTTGCACAACTTTTAAAGC+AGG	-	chr4_3:66090690-66090709	Msa0656730:intron	40.0%
	CAACGACTCTAAATAGCCTT+AGG	-	chr4_3:66090957-66090976	Msa0656730:intron	40.0%
	TGTGCTTGTTTCATTTCCAG+TGG	+	chr4_3:66091145-66091164	None:intergenic	40.0%
	GAAGGATGGACCCTAATTAA+AGG	+	chr4_3:66091406-66091425	None:intergenic	40.0%
	TAACACAGACAAGGAAAGGA+AGG	+	chr4_3:66091424-66091443	None:intergenic	40.0%
	ACGACCTTGATGTCTAACTA+CGG	+	chr4_3:66091482-66091501	None:intergenic	40.0%
	ATCAATCCAACCAATCAGGT+AGG	+	chr4_3:66091570-66091589	None:intergenic	40.0%
	CACCGACAAACTCCTTACTT+TGG	-	chr4_3:66089108-66089127	Msa0656730:three_prime_UTR	45.0%
	GACAAACTCCTTACTTTGGC+TGG	-	chr4_3:66089112-66089131	Msa0656730:three_prime_UTR	45.0%
	GAAGACAACCAGCCAAAGTA+AGG	+	chr4_3:66089123-66089142	None:intergenic	45.0%
	CCAGTGCAACAATCTATGGT+TGG	+	chr4_3:66089172-66089191	None:intergenic	45.0%
	CCAACCATAGATTGTTGCAC+TGG	-	chr4_3:66089169-66089188	Msa0656730:three_prime_UTR	45.0%
	GACACCAGTGCAACAATCTA+TGG	+	chr4_3:66089176-66089195	None:intergenic	45.0%
!	TTGTTGCACTGGTGTCAAAG+AGG	-	chr4_3:66089180-66089199	Msa0656730:three_prime_UTR	45.0%
	GATCATGATGACCCAAACCT+TGG	-	chr4_3:66089244-66089263	Msa0656730:three_prime_UTR	45.0%
!	CCTTTGGTAACTTAAGGGCA+AGG	+	chr4_3:66089288-66089307	None:intergenic	45.0%
	CCTTGCCCTTAAGTTACCAA+AGG	-	chr4_3:66089285-66089304	Msa0656730:three_prime_UTR	45.0%
	TACAAGGAAGTCAAGAAGGG+TGG	-	chr4_3:66089617-66089636	Msa0656730:intron	45.0%
	TATACAAACCTAGGCGTGGA+TGG	+	chr4_3:66089648-66089667	None:intergenic	45.0%
	GTCAGATATTCCGGAACGAA+GGG	+	chr4_3:66089902-66089921	None:intergenic	45.0%
	CTCAGATAAATTGGGACGGA+GGG	-	chr4_3:66090102-66090121	Msa0656730:intron	45.0%
!!	GTAGATTTTAACCAGTGCCC+CGG	-	chr4_3:66090596-66090615	Msa0656730:intron	45.0%
!!	TAGATTTTAACCAGTGCCCC+GGG	-	chr4_3:66090597-66090616	Msa0656730:intron	45.0%
!	AGCGTAGCATTGAAACTTCG+AGG	-	chr4_3:66090800-66090819	Msa0656730:intron	45.0%
!!	TTGAAACTTCGAGGTGTCGA+TGG	-	chr4_3:66090809-66090828	Msa0656730:intron	45.0%
	ATTGCTCAAGACAAGAACGG+TGG	-	chr4_3:66091165-66091184	Msa0656730:CDS	45.0%
	ACAGACAAGGAAAGGAAGGA+TGG	+	chr4_3:66091420-66091439	None:intergenic	45.0%
!	CTAACTACGGGACCCTTTTA+TGG	+	chr4_3:66091469-66091488	None:intergenic	45.0%
	CGACCTTGATGTCTAACTAC+GGG	+	chr4_3:66091481-66091500	None:intergenic	45.0%
!!!	CATATTTTATTAAAATATTT+AGG	-	chr4_3:66090367-66090386	Msa0656730:intron	5.0%
	CGGTGCATTCTGCTTTGTCT+TGG	+	chr4_3:66089093-66089112	None:intergenic	50.0%
	TGGAATTTCCATCCACGCCT+AGG	-	chr4_3:66089637-66089656	Msa0656730:intron	50.0%
	GTTAATCGAGCTGCCCTTAC+TGG	-	chr4_3:66089823-66089842	Msa0656730:intron	50.0%
	GGTCAGATATTCCGGAACGA+AGG	+	chr4_3:66089903-66089922	None:intergenic	50.0%
	GCTCAGATAAATTGGGACGG+AGG	-	chr4_3:66090101-66090120	Msa0656730:intron	50.0%
	AAATGTTAACCAGTGCCCCG+GGG	+	chr4_3:66090746-66090765	None:intergenic	50.0%
	GAAATGTTAACCAGTGCCCC+GGG	+	chr4_3:66090747-66090766	None:intergenic	50.0%
	GGAAATGTTAACCAGTGCCC+CGG	+	chr4_3:66090748-66090767	None:intergenic	50.0%
	ATTGCAGCTAGTGGTACCAC+TGG	-	chr4_3:66091126-66091145	Msa0656730:CDS	50.0%
	TTGTGGAAAGAGGTGGCTCT+TGG	-	chr4_3:66091191-66091210	Msa0656730:intron	50.0%
	AAAGAGGTGGCTCTTGGTAG+AGG	-	chr4_3:66091197-66091216	Msa0656730:intron	50.0%
!!	CTCTTGGTAGAGGTGCTTTG+TGG	-	chr4_3:66091207-66091226	Msa0656730:intron	50.0%
	GGTCCCGTAGTTAGACATCA+AGG	-	chr4_3:66091475-66091494	Msa0656730:CDS	50.0%
	TTTACCTGGAGGAACTGCGG+TGG	+	chr4_3:66089517-66089536	None:intergenic	55.0%
	ATTCCTTAACCAGTGGCCCG+GGG	+	chr4_3:66090616-66090635	None:intergenic	55.0%
	GATTCCTTAACCAGTGGCCC+GGG	+	chr4_3:66090617-66090636	None:intergenic	55.0%
	GGATTCCTTAACCAGTGGCC+CGG	+	chr4_3:66090618-66090637	None:intergenic	55.0%
	TGACTCCTTAACCAGTGCCC+CGG	-	chr4_3:66090726-66090745	Msa0656730:intron	55.0%
	GCTCAAGACAAGAACGGTGG+CGG	-	chr4_3:66091168-66091187	Msa0656730:CDS	55.0%
	ACTTCCACCGCAGTTCCTCC+AGG	-	chr4_3:66089510-66089529	Msa0656730:intron	60.0%
	GACTCCTTAACCAGTGCCCC+GGG	-	chr4_3:66090727-66090746	Msa0656730:intron	60.0%
	ACTCCTTAACCAGTGCCCCG+GGG	-	chr4_3:66090728-66090747	Msa0656730:intron	60.0%
!	GACAAGAACGGTGGCGGTTG+TGG	-	chr4_3:66091174-66091193	Msa0656730:intron	60.0%
!	ACGGTGGCGGTTGTGGAAAG+AGG	-	chr4_3:66091181-66091200	Msa0656730:intron	60.0%
	GTGGCGGTTGTGGAAAGAGG+TGG	-	chr4_3:66091184-66091203	Msa0656730:intron	60.0%
	TAACCAGTGCCCCGGGCCAC+TGG	-	chr4_3:66090604-66090623	Msa0656730:intron	70.0%
	TAACCAGTGGCCCGGGGCAC+TGG	+	chr4_3:66090610-66090629	None:intergenic	70.0%
!	GTGCCCCGGGCCACTGGTTA+AGG	-	chr4_3:66090610-66090629	Msa0656730:intron	70.0%
!!	GTGCCCCGGGGCACTGGTTA+AGG	+	chr4_3:66090740-66090759	None:intergenic	70.0%
	TAACCAGTGCCCCGGGGCAC+TGG	-	chr4_3:66090734-66090753	Msa0656730:intron	70.0%
	TAACCAGTGCCCCGGGGCAC+TGG	+	chr4_3:66090734-66090753	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	66088892	66091732	66088892	ID=Msa0656730;Name=Msa0656730
chr4_3	mRNA	66088892	66091732	66088892	ID=Msa0656730-mRNA-1;Parent=Msa0656730;Name=Msa0656730-mRNA-1;_AED=0.05;_eAED=0.05;_QI=109\|1\|1\|1\|1\|1\|3\|460\|192
chr4_3	exon	66088892	66089491	66088892	ID=Msa0656730-mRNA-1:exon:18970;Parent=Msa0656730-mRNA-1
chr4_3	exon	66091093	66091188	66091093	ID=Msa0656730-mRNA-1:exon:18969;Parent=Msa0656730-mRNA-1
chr4_3	exon	66091281	66091732	66091281	ID=Msa0656730-mRNA-1:exon:18968;Parent=Msa0656730-mRNA-1
chr4_3	five_prime_UTR	66091624	66091732	66091624	ID=Msa0656730-mRNA-1:five_prime_utr;Parent=Msa0656730-mRNA-1
chr4_3	CDS	66091281	66091623	66091281	ID=Msa0656730-mRNA-1:cds;Parent=Msa0656730-mRNA-1
chr4_3	CDS	66091093	66091188	66091093	ID=Msa0656730-mRNA-1:cds;Parent=Msa0656730-mRNA-1
chr4_3	CDS	66089352	66089491	66089352	ID=Msa0656730-mRNA-1:cds;Parent=Msa0656730-mRNA-1
chr4_3	three_prime_UTR	66088892	66089351	66088892	ID=Msa0656730-mRNA-1:three_prime_utr;Parent=Msa0656730-mRNA-1

Gene Sequence

>Msa0656730

ATGGGTTGCAAAAGCACAACAAATCTATCACTCTTCTCTACCTTGATTTTCCTAGTCCTTATGTTTGGTCTTGTAACCTCAGATATTAACCAAGACAAAGCAGAATGCACCGACAAACTCCTTACTTTGGCTGGTTGTCTTCCCTTTGTGACTAATCAAGCCAAATCTCCAACCATAGATTGTTGCACTGGTGTCAAAGAGGTTGTTGATAAGAGTAAGAGATGTTTATGTATCCTTATCAAAGATCATGATGACCCAAACCTTGGTTTAACTATAAATGTTACCCTTGCCCTTAAGTTACCAAAGGATTGTAACTCACCTACCAATATAACTCAATGTATTGATATTTTGCATTTGAAACCTAAATCACATGAGGCTAAGGTATTTGAGGACTTCCAAAAATCCTTGGAGAAAAATACTTCCACCGCAGTTCCTCCAGCTAGTGGTACCACTGGAAATGAAACAAGCACAATTGCTCAAGACAAGAACGGTGGCGGTTGTGGAAAGAGGTGGCTCTTGGTAGAGGTGCTTTGTGGCATTTTACCATTTGTTTTAGTTTCCTACTTCTTCGTAGTTTGA

Protein sequence

>Msa0656730

MGCKSTTNLSLFSTLIFLVLMFGLVTSDINQDKAECTDKLLTLAGCLPFVTNQAKSPTIDCCTGVKEVVDKSKRCLCILIKDHDDPNLGLTINVTLALKLPKDCNSPTNITQCIDILHLKPKSHEAKVFEDFQKSLEKNTSTAVPPASGTTGNETSTIAQDKNGGGCGKRWLLVEVLCGILPFVLVSYFFVV*