AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0708350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708350 MtrunA17_Chr8g0391311 93.911 542 31 2 1 541 1 541 0.0 1045
Msa0708350 MtrunA17_Chr8g0391291 93.358 542 32 3 1 541 1 539 0.0 1033
Msa0708350 MtrunA17_Chr8g0391281 91.667 312 22 3 1 311 1 309 0.0 560
Msa0708350 MtrunA17_Chr4g0011771 50.466 537 229 11 13 541 6 513 0.0 529
Msa0708350 MtrunA17_Chr4g0011791 50.000 534 226 13 18 541 7 509 2.12e-178 512
Msa0708350 MtrunA17_Chr3g0079341 46.887 514 243 7 36 541 36 527 8.52e-161 468
Msa0708350 MtrunA17_Chr4g0018371 44.942 514 261 6 36 541 40 539 5.67e-160 466
Msa0708350 MtrunA17_Chr3g0090091 46.911 518 238 10 36 541 36 528 1.93e-159 464
Msa0708350 MtrunA17_Chr3g0079361 46.498 514 245 6 36 541 36 527 4.26e-159 464
Msa0708350 MtrunA17_Chr3g0079351 46.693 514 244 8 36 541 36 527 2.37e-158 462
Msa0708350 MtrunA17_Chr4g0011751 49.895 475 204 8 74 541 42 489 1.65e-157 458
Msa0708350 MtrunA17_Chr7g0269541 45.946 518 252 7 35 537 48 552 7.23e-155 454
Msa0708350 MtrunA17_Chr7g0233771 45.920 527 244 11 37 541 47 554 2.30e-153 450
Msa0708350 MtrunA17_Chr8g0345281 44.571 525 267 6 35 541 71 589 3.38e-150 443
Msa0708350 MtrunA17_Chr2g0302021 47.269 476 217 7 79 541 71 525 2.70e-142 421
Msa0708350 MtrunA17_Chr7g0233801 43.570 521 254 10 42 541 52 553 1.22e-141 420
Msa0708350 MtrunA17_Chr1g0147051 42.214 533 272 10 35 541 85 607 1.32e-141 422
Msa0708350 MtrunA17_Chr3g0126771 42.430 535 262 11 35 541 81 597 1.94e-141 421
Msa0708350 MtrunA17_Chr8g0354901 50.107 467 200 9 88 541 135 581 3.22e-141 420
Msa0708350 MtrunA17_Chr1g0192331 45.889 523 242 14 35 541 31 528 3.76e-140 415
Msa0708350 MtrunA17_Chr7g0269531 44.860 535 252 11 33 537 40 561 5.22e-140 416
Msa0708350 MtrunA17_Chr7g0233831 45.510 490 238 8 57 541 90 555 1.10e-139 415
Msa0708350 MtrunA17_Chr4g0069841 45.929 479 214 8 81 541 65 516 1.28e-137 408
Msa0708350 MtrunA17_Chr7g0272141 45.051 495 234 10 63 541 70 542 4.85e-137 408
Msa0708350 MtrunA17_Chr7g0269511 42.938 531 272 8 35 541 41 564 1.01e-134 402
Msa0708350 MtrunA17_Chr7g0269521 50.698 430 190 8 129 541 138 562 1.09e-134 402
Msa0708350 MtrunA17_Chr4g0069891 42.344 529 268 10 33 541 82 593 1.52e-133 400
Msa0708350 MtrunA17_Chr3g0080391 46.753 462 215 8 92 541 132 574 2.64e-133 399
Msa0708350 MtrunA17_Chr1g0195331 42.832 558 275 12 18 541 8 555 6.46e-132 395
Msa0708350 MtrunA17_Chr1g0195301 42.473 558 277 12 18 541 8 555 2.31e-131 394
Msa0708350 MtrunA17_Chr1g0195321 42.473 558 277 12 18 541 8 555 2.31e-131 394
Msa0708350 MtrunA17_Chr4g0069881 42.418 521 256 13 36 541 44 535 9.02e-131 391
Msa0708350 MtrunA17_Chr8g0345211 43.429 525 255 13 36 541 81 582 2.03e-130 392
Msa0708350 MtrunA17_Chr8g0354911 41.848 552 260 12 35 541 77 612 3.91e-130 392
Msa0708350 MtrunA17_Chr4g0069861 43.763 473 235 6 81 541 64 517 6.95e-130 389
Msa0708350 MtrunA17_Chr1g0175661 42.495 553 277 13 21 541 15 558 3.72e-129 388
Msa0708350 MtrunA17_Chr1g0195351 42.115 558 277 13 18 541 8 553 1.65e-126 381
Msa0708350 MtrunA17_Chr1g0194091 40.516 543 288 12 27 541 34 569 1.17e-124 377
Msa0708350 MtrunA17_Chr1g0194081 38.534 532 302 11 30 541 37 563 2.29e-120 366
Msa0708350 MtrunA17_Chr1g0195311 61.597 263 100 1 279 541 53 314 3.99e-119 353
Msa0708350 MtrunA17_Chr3g0080381 43.991 441 219 7 124 541 193 628 1.60e-118 363
Msa0708350 MtrunA17_Chr1g0192311 39.306 519 284 14 36 537 62 566 4.01e-112 345
Msa0708350 MtrunA17_Chr1g0192281 39.306 519 284 14 36 537 62 566 4.01e-112 345
Msa0708350 MtrunA17_Chr1g0192321 37.753 543 280 14 36 540 60 582 1.84e-111 343
Msa0708350 MtrunA17_Chr7g0272151 38.131 535 286 12 36 540 63 582 3.08e-111 343
Msa0708350 MtrunA17_Chr1g0192301 37.958 519 291 12 36 537 67 571 1.52e-110 341
Msa0708350 MtrunA17_Chr4g0067611 39.347 521 288 12 36 540 63 571 2.75e-110 340
Msa0708350 MtrunA17_Chr4g0067601 38.973 526 289 12 33 540 60 571 9.43e-110 339
Msa0708350 MtrunA17_Chr4g0000121 37.572 519 293 12 36 537 49 553 1.62e-109 338
Msa0708350 MtrunA17_Chr7g0270661 39.715 491 263 9 75 536 83 569 7.43e-106 328
Msa0708350 MtrunA17_Chr7g0270671 39.715 491 263 9 75 536 83 569 7.43e-106 328
Msa0708350 MtrunA17_Chr2g0301931 54.225 284 125 3 258 541 14 292 7.53e-105 316
Msa0708350 MtrunA17_Chr7g0270651 36.431 538 307 10 33 541 43 574 2.00e-103 322
Msa0708350 MtrunA17_Chr2g0295531 36.069 524 291 12 33 537 44 542 2.23e-103 323
Msa0708350 MtrunA17_Chr7g0234711 62.281 228 85 1 315 541 42 269 5.86e-103 310
Msa0708350 MtrunA17_Chr7g0270691 35.057 522 299 9 36 541 52 549 3.47e-100 313
Msa0708350 MtrunA17_Chr2g0302001 53.543 254 117 1 288 541 185 437 4.97e-99 306
Msa0708350 MtrunA17_Chr2g0295551 36.295 529 294 11 33 540 57 563 9.14e-99 310
Msa0708350 MtrunA17_Chr7g0270631 38.557 485 269 8 75 532 71 553 1.28e-94 311
Msa0708350 MtrunA17_Chr7g0270631 37.917 480 266 9 75 524 619 1096 5.15e-84 282
Msa0708350 MtrunA17_Chr8g0391261 92.806 139 10 0 403 541 28 166 1.07e-91 278
Msa0708350 MtrunA17_Chr8g0384121 34.351 524 303 13 31 533 53 556 1.98e-90 288
Msa0708350 MtrunA17_Chr3g0129921 34.879 539 284 16 33 534 49 557 6.74e-90 287
Msa0708350 MtrunA17_Chr2g0295541 33.451 565 292 14 33 540 57 594 1.03e-89 287
Msa0708350 MtrunA17_Chr5g0437831 41.813 342 192 6 202 541 47 383 1.28e-83 265
Msa0708350 MtrunA17_Chr8g0380581 39.763 337 193 4 203 537 44 372 1.59e-83 264
Msa0708350 MtrunA17_Chr7g0268241 40.256 313 174 5 230 541 50 350 1.87e-76 245
Msa0708350 MtrunA17_Chr2g0334111 38.535 314 178 5 229 541 24 323 4.51e-75 240
Msa0708350 MtrunA17_Chr7g0271381 31.530 536 294 17 36 541 64 556 3.66e-68 229
Msa0708350 MtrunA17_Chr6g0487101 39.085 284 147 7 258 530 112 380 2.67e-50 177
Msa0708350 MtrunA17_Chr8g0389771 38.811 286 147 7 258 530 107 377 1.75e-49 175
Msa0708350 MtrunA17_Chr1g0194311 32.733 333 193 8 208 530 57 368 2.11e-49 174
Msa0708350 MtrunA17_Chr2g0299431 43.265 245 124 6 203 442 30 264 8.35e-49 170
Msa0708350 MtrunA17_Chr6g0453551 33.770 305 177 6 230 524 6 295 1.22e-48 171
Msa0708350 MtrunA17_Chr7g0258341 34.421 337 194 7 209 531 65 388 4.09e-48 171
Msa0708350 MtrunA17_Chr6g0466951 34.722 288 164 6 221 503 60 328 6.97e-48 170
Msa0708350 MtrunA17_Chr6g0466961 36.140 285 154 7 226 503 36 299 7.65e-46 164
Msa0708350 MtrunA17_Chr7g0217941 32.099 324 189 7 232 539 8 316 7.90e-46 163
Msa0708350 MtrunA17_Chr1g0161731 32.624 282 159 6 261 534 79 337 1.43e-42 155
Msa0708350 MtrunA17_Chr7g0234731 33.688 282 158 6 36 310 55 314 5.03e-42 153
Msa0708350 MtrunA17_Chr5g0446301 32.899 307 178 7 236 530 67 357 4.85e-38 143
Msa0708350 MtrunA17_Chr4g0070051 31.833 311 181 12 230 530 54 343 1.30e-37 142
Msa0708350 MtrunA17_Chr5g0437821 46.970 132 69 1 344 474 4 135 1.89e-35 129
Msa0708350 MtrunA17_Chr5g0440871 31.270 307 186 7 227 531 36 319 2.10e-35 135
Msa0708350 MtrunA17_Chr7g0265201 29.412 306 188 6 232 530 101 385 5.75e-35 135
Msa0708350 MtrunA17_Chr5g0399221 29.483 329 200 8 223 539 56 364 5.01e-34 132
Msa0708350 MtrunA17_Chr2g0294321 29.231 325 204 7 212 530 42 346 6.33e-34 131
Msa0708350 MtrunA17_Chr8g0351871 29.114 316 195 8 225 531 28 323 1.75e-31 124
Msa0708350 MtrunA17_Chr4g0037751 29.964 277 175 6 230 500 71 334 2.98e-30 121
Msa0708350 MtrunA17_Chr6g0459781 38.728 173 93 4 230 392 13 182 7.01e-30 116
Msa0708350 MtrunA17_Chr2g0295851 28.221 326 197 10 212 530 41 336 8.14e-29 117
Msa0708350 MtrunA17_Chr5g0420831 29.474 285 170 8 230 502 71 336 3.35e-28 115
Msa0708350 MtrunA17_Chr4g0025841 29.388 245 155 5 230 474 37 263 1.46e-25 106
Msa0708350 MtrunA17_Chr5g0440901 33.553 152 91 2 323 474 1 142 8.85e-23 94.7
Msa0708350 MtrunA17_Chr7g0265161 30.387 181 93 4 296 471 5 157 1.08e-20 91.3
Msa0708350 MtrunA17_Chr2g0314691 28.788 198 126 4 334 530 18 201 4.85e-20 88.6
Msa0708350 MtrunA17_Chr6g0460141 37.681 138 78 5 261 390 7 144 9.08e-19 83.6
Msa0708350 MtrunA17_Chr5g0440891 33.333 156 96 3 230 385 48 195 2.80e-17 80.5
Msa0708350 MtrunA17_Chr4g0073911 27.273 176 118 3 261 429 38 210 6.72e-16 77.8
Msa0708350 MtrunA17_Chr6g0459791 36.000 125 67 4 407 530 4 116 1.33e-14 70.5
Msa0708350 MtrunA17_Chr4g0018241 28.313 166 103 5 27 181 35 195 5.92e-11 62.0
Msa0708350 MtrunA17_Chr4g0018251 28.313 166 103 5 27 181 35 195 5.98e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0708350 AT1G23200.1 52.603 557 225 13 11 541 11 554 0.0 551
Msa0708350 AT2G45220.1 50.196 510 218 11 36 541 34 511 1.47e-173 500
Msa0708350 AT5G51490.1 43.378 521 258 9 36 541 38 536 1.59e-148 437
Msa0708350 AT3G49220.1 45.211 522 256 13 35 541 90 596 3.02e-148 439
Msa0708350 AT5G51500.1 44.038 520 256 10 36 541 42 540 1.09e-147 435
Msa0708350 AT3G49220.3 45.211 522 256 13 35 541 135 641 1.37e-147 439
Msa0708350 AT3G60730.1 45.455 495 238 7 70 541 34 519 2.45e-147 434
Msa0708350 AT3G47400.1 43.403 523 263 8 36 541 88 594 1.02e-145 432
Msa0708350 AT5G53370.1 44.208 518 268 10 35 541 78 585 2.38e-145 431
Msa0708350 AT3G14310.1 43.993 541 259 9 35 541 62 592 2.55e-145 431
Msa0708350 AT5G53370.2 44.208 518 268 10 35 541 114 621 5.46e-145 431
Msa0708350 AT1G11580.1 44.444 504 259 6 45 541 68 557 2.33e-142 422
Msa0708350 AT1G11580.2 44.444 504 259 6 45 541 89 578 4.22e-142 422
Msa0708350 AT1G53830.1 42.884 534 265 8 36 541 66 587 5.71e-140 417
Msa0708350 AT3G59010.1 46.377 483 226 11 69 541 69 528 1.94e-137 409
Msa0708350 AT3G43270.1 45.979 485 241 6 69 541 50 525 1.71e-135 404
Msa0708350 AT2G26440.1 42.553 517 276 8 36 541 41 547 5.63e-134 400
Msa0708350 AT4G02330.1 42.355 569 277 12 18 541 9 571 8.50e-134 401
Msa0708350 AT4G00190.1 42.775 519 247 11 28 541 1 474 1.59e-133 397
Msa0708350 AT5G27870.1 41.973 517 274 11 36 541 61 562 3.71e-132 402
Msa0708350 AT2G26450.1 41.973 517 271 9 36 541 113 611 4.11e-132 398
Msa0708350 AT4G02320.1 45.614 456 228 5 96 541 73 518 7.24e-132 394
Msa0708350 AT3G05620.1 41.935 527 261 9 35 541 40 541 7.09e-131 392
Msa0708350 AT4G02300.1 44.035 461 222 7 98 541 91 532 1.64e-130 391
Msa0708350 AT2G43050.1 45.843 445 207 7 103 541 100 516 1.96e-130 390
Msa0708350 AT4G33220.1 42.797 472 236 6 94 541 62 523 8.64e-130 389
Msa0708350 AT3G05610.1 41.154 520 276 11 36 541 62 565 3.51e-129 392
Msa0708350 AT5G04960.1 41.617 507 277 9 36 539 72 562 5.66e-129 388
Msa0708350 AT3G10710.1 41.748 515 264 9 36 541 74 561 1.06e-125 380
Msa0708350 AT1G02810.1 55.521 317 137 3 228 541 262 577 9.09e-124 375
Msa0708350 AT5G04970.1 40.367 545 278 13 34 541 86 620 9.36e-124 377
Msa0708350 AT3G10720.2 39.147 539 291 9 35 541 82 615 1.58e-123 376
Msa0708350 AT4G33220.2 50.737 339 161 2 203 541 70 402 2.60e-123 368
Msa0708350 AT2G47550.1 45.322 481 230 10 88 541 84 558 5.35e-123 373
Msa0708350 AT4G33230.1 40.385 520 284 10 36 541 99 606 1.16e-122 373
Msa0708350 AT5G49180.1 40.535 523 281 13 33 537 56 566 2.33e-122 371
Msa0708350 AT3G06830.1 39.771 523 285 13 36 539 55 566 1.34e-121 369
Msa0708350 AT5G20860.2 39.469 527 271 12 36 541 39 538 3.53e-121 367
Msa0708350 AT1G53840.1 39.658 527 269 14 36 535 77 581 4.64e-121 369
Msa0708350 AT3G14300.1 40.190 525 269 14 36 536 463 966 4.23e-114 361
Msa0708350 AT3G10720.1 56.923 260 111 1 282 541 1 259 1.35e-108 325
Msa0708350 AT4G03930.1 36.111 540 303 11 20 541 9 524 5.13e-106 328
Msa0708350 AT3G27980.1 47.774 337 168 3 205 541 169 497 1.12e-103 321
Msa0708350 AT1G11590.1 40.135 446 238 7 106 541 98 524 1.44e-102 319
Msa0708350 AT4G15980.1 47.337 338 174 4 203 539 366 700 1.30e-101 322
Msa0708350 AT4G15980.2 47.337 338 174 4 203 539 376 710 2.15e-101 321
Msa0708350 AT5G20860.1 36.240 516 251 12 28 520 29 489 5.67e-100 311
Msa0708350 AT5G09760.1 34.463 531 294 12 33 534 46 551 3.51e-92 293
Msa0708350 AT5G09760.2 34.463 531 294 12 33 534 72 577 4.74e-92 293
Msa0708350 AT1G11370.1 49.612 258 129 1 216 473 32 288 1.56e-87 272
Msa0708350 AT2G47030.1 40.049 412 216 8 154 541 184 588 2.78e-86 278
Msa0708350 AT3G62170.1 37.131 474 257 11 100 541 124 588 6.77e-86 277
Msa0708350 AT2G47040.1 39.207 454 248 12 103 541 155 595 7.87e-86 277
Msa0708350 AT5G64640.1 36.748 449 250 11 104 531 164 599 9.84e-85 275
Msa0708350 AT5G19730.1 37.676 284 151 6 258 530 113 381 2.65e-50 177
Msa0708350 AT3G29090.1 35.439 285 160 6 230 503 7 278 3.58e-48 170
Msa0708350 AT1G05310.1 35.032 314 175 7 230 530 91 388 4.61e-47 169
Msa0708350 AT2G36710.1 33.442 308 185 5 230 530 91 385 3.70e-44 161
Msa0708350 AT2G36700.1 31.579 304 189 5 232 530 44 333 4.87e-44 159
Msa0708350 AT1G44980.1 42.512 207 117 2 188 394 37 241 3.04e-42 152
Msa0708350 AT2G21610.1 30.795 302 188 6 230 530 51 332 6.14e-40 149
Msa0708350 AT3G17060.1 28.869 336 205 8 210 530 20 336 7.40e-40 148
Msa0708350 AT2G21610.2 30.795 302 188 6 230 530 56 337 8.73e-40 148
Msa0708350 AT3G24130.1 31.333 300 179 7 215 503 21 304 8.44e-39 145
Msa0708350 AT5G18990.1 32.000 300 172 7 215 503 21 299 1.80e-37 141
Msa0708350 AT5G47500.1 30.699 329 196 8 223 539 54 362 2.60e-37 142
Msa0708350 AT5G07430.1 30.323 310 196 6 225 530 62 355 4.54e-37 141
Msa0708350 AT5G55590.1 34.385 317 178 9 227 530 79 378 5.99e-36 138
Msa0708350 AT5G26810.1 30.794 315 191 9 230 531 34 334 2.47e-34 133
Msa0708350 AT5G55590.2 34.615 260 145 7 227 474 79 325 4.68e-34 132
Msa0708350 AT3G49220.2 32.680 306 173 14 35 325 135 422 2.01e-33 132
Msa0708350 AT5G61680.1 29.052 327 206 9 209 530 53 358 8.89e-33 129
Msa0708350 AT2G19150.1 28.395 324 209 8 221 537 32 339 1.87e-32 127
Msa0708350 AT5G07420.1 28.308 325 207 9 213 530 50 355 3.11e-31 124
Msa0708350 AT2G47280.1 29.167 312 193 8 230 531 35 328 6.70e-30 120
Msa0708350 AT2G19150.2 29.344 259 167 6 221 474 32 279 3.20e-28 114
Msa0708350 AT1G69940.1 26.045 311 207 8 225 530 63 355 6.19e-27 112
Msa0708350 AT5G07410.1 26.367 311 206 8 225 530 63 355 9.53e-27 112

Find 150 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGTGATTCTTGGATTTC+AGG 0.138272 4_4:-81891098 Msa0708350:CDS
AAATTTCCATACTGGCTTTC+AGG 0.138802 4_4:-81893494 Msa0708350:CDS
GTGAAGAATTTAATGATATT+TGG 0.168927 4_4:-81893269 Msa0708350:CDS
TTCTTCTTCTTTCCATGATA+TGG 0.204942 4_4:-81893918 Msa0708350:CDS
AATAATTATTGCAGCTGTTA+TGG 0.230375 4_4:-81891750 Msa0708350:intron
GCCAAGTTAATTTATCATTT+AGG 0.239565 4_4:+81893732 None:intergenic
TGTGAATATTGATTAATTTC+TGG 0.257072 4_4:-81891049 Msa0708350:exon
GCATTGAGTACACTTTATTA+TGG 0.267204 4_4:-81891236 Msa0708350:CDS
AATCATCAAACTTGTCAAAA+TGG 0.271720 4_4:-81893692 Msa0708350:CDS
ACAATCTTCCCATGCTGATT+TGG 0.282004 4_4:+81893811 None:intergenic
TTTCCATGATATGGCTCTTA+AGG 0.287787 4_4:-81893909 Msa0708350:CDS
AATCATTTGCTGCGGTTATA+CGG 0.315468 4_4:+81891393 None:intergenic
AATATGAGTTTATTCATATT+AGG 0.319561 4_4:+81894082 None:intergenic
CTGGTGATTCTTGGATTTCA+GGG 0.333928 4_4:-81891097 Msa0708350:CDS
ATAATTATTGCAGCTGTTAT+GGG 0.345443 4_4:-81891749 Msa0708350:intron
TCATCAAGTACTAAACAAAA+TGG 0.356786 4_4:-81893539 Msa0708350:CDS
ATGGACTCCACTATTGTTAC+TGG 0.363587 4_4:-81893239 Msa0708350:CDS
GGAACTATTGATTTCATCTT+CGG 0.369134 4_4:-81891545 Msa0708350:CDS
CATGGAAAGAAGAAGAATCT+AGG 0.371037 4_4:+81893924 None:intergenic
TATCAGATAAATTTCCATAC+TGG 0.372948 4_4:-81893502 Msa0708350:CDS
GTCAGATAGAAAGCTGCTTC+AGG 0.393140 4_4:-81893471 Msa0708350:CDS
TCTGACCCTGAAAGCCAGTA+TGG 0.394838 4_4:+81893488 None:intergenic
CTTCAGGCAACACCAGGGTC+AGG 0.401448 4_4:-81893455 Msa0708350:CDS
AATTTCCATACTGGCTTTCA+GGG 0.402050 4_4:-81893493 Msa0708350:CDS
TTTGATAAATTCAGAAGGTT+GGG 0.405145 4_4:-81891279 Msa0708350:CDS
GGTAATCATAATGCTATAGA+TGG 0.406520 4_4:-81893218 Msa0708350:CDS
TGTTGCAGTCGCGGTAGAAT+TGG 0.411073 4_4:+81891573 None:intergenic
GTTGTCATTCATGTGAAATC+AGG 0.412213 4_4:-81893326 Msa0708350:CDS
TGTTCTTCCTTGTGCTGTTA+CGG 0.414972 4_4:+81891454 None:intergenic
ATACTGGTGGAAGAGTTAAT+TGG 0.417090 4_4:-81891184 Msa0708350:CDS
GTAATGAAAAGTAATATTGA+TGG 0.420796 4_4:-81891302 Msa0708350:CDS
TATTGTCATGTAGTATTTGT+TGG 0.422147 4_4:-81890883 Msa0708350:three_prime_UTR
TTTGGAAGAGCTAGGTATAT+AGG 0.422385 4_4:+81894154 None:intergenic
AGACAGTGTGTTGGTGGTGG+TGG 0.423692 4_4:+81893949 None:intergenic
GCGGTTGGCGTAAGCATATA+AGG 0.426322 4_4:+81891595 None:intergenic
CGCAGCAAATGATTTGAAAC+CGG 0.431620 4_4:-81891384 Msa0708350:CDS
TACAAGTTTGTGTGCTTCAA+TGG 0.434838 4_4:+81893871 None:intergenic
GTTGGTGGTGGTGCTAATAC+TGG 0.437055 4_4:-81891200 Msa0708350:CDS
CTTGGTGCTGGCCCTGACCC+TGG 0.440453 4_4:+81893443 None:intergenic
CAAGCAGTAGCTCTCCGTTC+CGG 0.442034 4_4:-81891668 Msa0708350:CDS
GGGAAGTAGTTTAAGTGAGA+TGG 0.442427 4_4:+81893650 None:intergenic
GGTGTCTTGGTAGCCTTTGA+AGG 0.444283 4_4:+81891616 None:intergenic
GTTTGATAAATTCAGAAGGT+TGG 0.445073 4_4:-81891280 Msa0708350:CDS
TTTGAAATTATTTAATTCCA+TGG 0.450557 4_4:+81893844 None:intergenic
GTGTTCTGTTGGTTGCTCAT+TGG 0.453378 4_4:+81891476 None:intergenic
TGGTTACACACATGAGGGTA+TGG 0.456641 4_4:+81893983 None:intergenic
GTGAAGTTGCTTAACATGGA+TGG 0.463236 4_4:+81893629 None:intergenic
ATTCAGAAGGTTGGGCTCCA+TGG 0.464333 4_4:-81891271 Msa0708350:CDS
ATTGCAGCTGTTATGGGAGA+TGG 0.472604 4_4:-81891743 Msa0708350:intron
ATTGTCATGTAGTATTTGTT+GGG 0.472757 4_4:-81890882 Msa0708350:three_prime_UTR
GGACATGACCTTTGAGAATA+CGG 0.477411 4_4:-81891708 Msa0708350:CDS
AATGATATTTGGAGATGGAA+TGG 0.477734 4_4:-81893258 Msa0708350:CDS
GTTGCAGCTGCTAAGCTTAG+TGG 0.477922 4_4:-81893359 Msa0708350:CDS
GAAGGTTGGGCTCCATGGAT+GGG 0.479662 4_4:-81891266 Msa0708350:CDS
GTTTGTGTGCTTCAATGGCT+TGG 0.481327 4_4:+81893876 None:intergenic
AGAAGGTTGGGCTCCATGGA+TGG 0.481727 4_4:-81891267 Msa0708350:CDS
AAGCTGCTTCAGGCAACACC+AGG 0.481870 4_4:-81893461 Msa0708350:CDS
ACAAGTTTGATGATTGGTAA+TGG 0.484728 4_4:+81893700 None:intergenic
TGCTTCAATGGCTTGGTCCA+TGG 0.489763 4_4:+81893883 None:intergenic
TTCTACCGTTGTGCCTTCAA+AGG 0.490213 4_4:-81891629 Msa0708350:CDS
ACTTGAAATGATGATGACTT+TGG 0.499085 4_4:-81890969 Msa0708350:three_prime_UTR
AGATTGTTTAGAGCTTTATG+AGG 0.504717 4_4:-81893795 Msa0708350:CDS
AGTGGTGTGCCGTTTGATGC+AGG 0.508356 4_4:-81891074 Msa0708350:CDS
ACCTAAATGATAAATTAACT+TGG 0.508748 4_4:-81893733 Msa0708350:CDS
ACAATATCAGCTCTTGGTGC+TGG 0.510769 4_4:+81893431 None:intergenic
TGCAGTCGCGGTAGAATTGG+CGG 0.511251 4_4:+81891576 None:intergenic
TGAAGTTGCTTAACATGGAT+GGG 0.511299 4_4:+81893630 None:intergenic
TTGCAGCTGCTAAGCTTAGT+GGG 0.511688 4_4:-81893358 Msa0708350:CDS
GGTAGACAGTGTGTTGGTGG+TGG 0.512332 4_4:+81893946 None:intergenic
GATATTGTTGTTGCACAAGA+TGG 0.515434 4_4:-81893416 Msa0708350:CDS
TGGTGGTGGTTCCAATGTAA+TGG 0.517543 4_4:+81893963 None:intergenic
GTTCTTGAATATTTCTGCCA+TGG 0.518439 4_4:+81891329 None:intergenic
GGTGGAAGAGTTAATTGGCC+TGG 0.520232 4_4:-81891179 Msa0708350:CDS
ATGATTACCAGTAACAATAG+TGG 0.522826 4_4:+81893232 None:intergenic
ATGGAAAGAAGAAGAATCTA+GGG 0.522868 4_4:+81893925 None:intergenic
GGGAGATGGTTTCATAGCTA+AGG 0.523196 4_4:-81891729 Msa0708350:CDS
GAAGGCACAACGGTAGAAAA+CGG 0.523739 4_4:+81891634 None:intergenic
ATTCACAAACCTGCATCAAA+CGG 0.526344 4_4:+81891065 None:intergenic
TTTAGTGAAGTTGCTTAACA+TGG 0.527598 4_4:+81893625 None:intergenic
TACCGCGACTGCAACATCTA+CGG 0.528232 4_4:-81891566 Msa0708350:CDS
TGACCTTAAGAGCCATATCA+TGG 0.529885 4_4:+81893906 None:intergenic
CGTAAGCATATAAGGTGTCT+TGG 0.533953 4_4:+81891603 None:intergenic
TATAGTGAAAGAAATAAAAG+TGG 0.536866 4_4:-81891011 Msa0708350:three_prime_UTR
TGTTATTACTAATCCTTCTG+AGG 0.537907 4_4:-81891150 Msa0708350:CDS
GTGCTGTTACGGTGTTCTGT+TGG 0.539536 4_4:+81891465 None:intergenic
GGTTGGGCTCCATGGATGGG+TGG 0.540114 4_4:-81891263 Msa0708350:CDS
TGAAGTTAGAGTGTTGCTAA+TGG 0.541067 4_4:+81893583 None:intergenic
TATGGTGAATATATGAATGT+TGG 0.547151 4_4:-81891218 Msa0708350:CDS
TGGGTGTTTGCCTTGTACAA+AGG 0.550451 4_4:-81890863 Msa0708350:three_prime_UTR
TCTTGTGTAACCTTTGTACA+AGG 0.550590 4_4:+81890853 None:intergenic
GGTTACACACATGAGGGTAT+GGG 0.553090 4_4:+81893984 None:intergenic
GAAAATTTAACAGCCTCAGA+AGG 0.553132 4_4:+81891137 None:intergenic
AATTTAATGATATTTGGAGA+TGG 0.553852 4_4:-81893263 Msa0708350:CDS
CAGAACACCGTAACAGCACA+AGG 0.556039 4_4:-81891461 Msa0708350:CDS
GCTGCTAAGCTTAGTGGGAA+AGG 0.557812 4_4:-81893353 Msa0708350:CDS
GATTCTTGGATTTCAGGGAG+TGG 0.559486 4_4:-81891092 Msa0708350:CDS
CGGAGTGATCGGCGCCGGAA+CGG 0.559791 4_4:+81891654 None:intergenic
GCAACAACAATATCAGCTCT+TGG 0.560130 4_4:+81893425 None:intergenic
AGTACTTGATGATGTGGATG+AGG 0.565120 4_4:+81893550 None:intergenic
TTGTTTAGTACTTGATGATG+TGG 0.565122 4_4:+81893544 None:intergenic
GTTGTTGCACAAGATGGAAG+TGG 0.568022 4_4:-81893410 Msa0708350:CDS
AAAAGTGGTTGATGATTCAG+TGG 0.568443 4_4:-81890996 Msa0708350:three_prime_UTR
ACGGTAGAAAACGGAGTGAT+CGG 0.568769 4_4:+81891643 None:intergenic
CATGTGTGTAACCATTACAT+TGG 0.569476 4_4:-81893974 Msa0708350:CDS
GGGTCCTGCCGTATTCTCAA+AGG 0.569689 4_4:+81891700 None:intergenic
GTCGCGGTAGAATTGGCGGT+TGG 0.570719 4_4:+81891580 None:intergenic
AGTAGTTTAAGTGAGATGGA+AGG 0.574197 4_4:+81893654 None:intergenic
GTTACACACATGAGGGTATG+GGG 0.574452 4_4:+81893985 None:intergenic
TAGGGTAGACAGTGTGTTGG+TGG 0.577198 4_4:+81893943 None:intergenic
CGGAACGGAGAGCTACTGCT+TGG 0.579824 4_4:+81891669 None:intergenic
GATGGTTTGATAAATTCAGA+AGG 0.580230 4_4:-81891284 Msa0708350:CDS
TAAATATATTTGGAAGAGCT+AGG 0.580761 4_4:+81894146 None:intergenic
AATGCAAAACCACCCATCCA+TGG 0.580980 4_4:+81891254 None:intergenic
AAGAGCTAGGTATATAGGAA+TGG 0.581001 4_4:+81894159 None:intergenic
TGCAACAACACCCTCTGAGA+TGG 0.581283 4_4:+81893376 None:intergenic
GAAAACGGAGTGATCGGCGC+CGG 0.581382 4_4:+81891649 None:intergenic
TTCCGTAGATGTTGCAGTCG+CGG 0.586032 4_4:+81891564 None:intergenic
TCAAGTACTAAACAAAATGG+AGG 0.588650 4_4:-81893536 Msa0708350:CDS
AAAAGAGGATGGTGAAAGCG+TGG 0.591353 4_4:-81890931 Msa0708350:three_prime_UTR
GGTGAATATATGAATGTTGG+TGG 0.591996 4_4:-81891215 Msa0708350:CDS
TGGTGGTGACAATTGAAGAT+GGG 0.594007 4_4:-81890911 Msa0708350:three_prime_UTR
ACAAGCGTGCCAAATCAGCA+TGG 0.597886 4_4:-81893820 Msa0708350:CDS
AGGATATGCAATATTGTGCA+TGG 0.599200 4_4:+81894102 None:intergenic
AACTATAAGACCATCTCAGA+GGG 0.599247 4_4:-81893386 Msa0708350:CDS
ATGACCTTTGAGAATACGGC+AGG 0.599978 4_4:-81891704 Msa0708350:CDS
GTGGTGGTGACAATTGAAGA+TGG 0.602928 4_4:-81890912 Msa0708350:three_prime_UTR
TTCAGGCAACACCAGGGTCA+GGG 0.603563 4_4:-81893454 Msa0708350:CDS
CAAGCGTGCCAAATCAGCAT+GGG 0.604901 4_4:-81893819 Msa0708350:CDS
ATGTAATGGTTACACACATG+AGG 0.605826 4_4:+81893977 None:intergenic
GATGATGTGGATGAGGATGA+TGG 0.609727 4_4:+81893557 None:intergenic
ATCTAGGGTAGACAGTGTGT+TGG 0.618112 4_4:+81893940 None:intergenic
CTAAACAAAATGGAGGGAGA+AGG 0.620123 4_4:-81893529 Msa0708350:CDS
GAATATATGAATGTTGGTGG+TGG 0.621736 4_4:-81891212 Msa0708350:CDS
CGGTTTCAAATCATTTGCTG+CGG 0.623680 4_4:+81891385 None:intergenic
CAAGTACTAAACAAAATGGA+GGG 0.628274 4_4:-81893535 Msa0708350:CDS
GGTATATAGGAATGGATGAG+TGG 0.630123 4_4:+81894167 None:intergenic
AAACATATCTTGGTAGACCA+TGG 0.637768 4_4:-81891346 Msa0708350:CDS
TGTAATGGTTACACACATGA+GGG 0.637885 4_4:+81893978 None:intergenic
ACAGATCCAAATGAAAACAC+AGG 0.641536 4_4:-81891434 Msa0708350:CDS
AAAGTGGTTGATGATTCAGT+GGG 0.655206 4_4:-81890995 Msa0708350:three_prime_UTR
GGTGGTGGTGCTAATACTGG+TGG 0.661832 4_4:-81891197 Msa0708350:CDS
ATGGCAGAAATATTCAAGAA+CGG 0.671890 4_4:-81891327 Msa0708350:CDS
TTAGTAATAACATGAAAACC+AGG 0.674041 4_4:+81891161 None:intergenic
ACACAAACTTGTATCAACCA+TGG 0.676347 4_4:-81893861 Msa0708350:CDS
GGTGGTGACAATTGAAGATG+GGG 0.677712 4_4:-81890910 Msa0708350:three_prime_UTR
TATGGCTCTTAAGGTCACCA+TGG 0.681394 4_4:-81893900 Msa0708350:CDS
GGTAGCCTTTGAAGGCACAA+CGG 0.688392 4_4:+81891624 None:intergenic
AGCTGCTTCAGGCAACACCA+GGG 0.695457 4_4:-81893460 Msa0708350:CDS
AAACTATAAGACCATCTCAG+AGG 0.701800 4_4:-81893387 Msa0708350:CDS
AGAGGATGGTGAAAGCGTGG+TGG 0.727927 4_4:-81890928 Msa0708350:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTATTTTTTTTTTTTT+GGG - chr4_4:81894112-81894131 Msa0708350:exon 0.0%
!!! TTTTAATGGCTAAATATATT+TGG + chr4_4:81890785-81890804 None:intergenic 15.0%
!! AATATGAGTTTATTCATATT+AGG + chr4_4:81890839-81890858 None:intergenic 15.0%
!!! TTTGAAATTATTTAATTCCA+TGG + chr4_4:81891077-81891096 None:intergenic 15.0%
!! AACAAATGTTAAATAAAGTA+GGG + chr4_4:81892150-81892169 None:intergenic 15.0%
!! AAACAAATGTTAAATAAAGT+AGG + chr4_4:81892151-81892170 None:intergenic 15.0%
!!! TAAGAAAAGTGTATAAATAA+AGG - chr4_4:81892519-81892538 Msa0708350:intron 15.0%
!!! AAGAAAAGTGTATAAATAAA+GGG - chr4_4:81892520-81892539 Msa0708350:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr4_4:81892584-81892603 Msa0708350:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG - chr4_4:81892585-81892604 Msa0708350:intron 15.0%
!! TTTAGTGAATATTGTTAAAA+AGG + chr4_4:81892712-81892731 None:intergenic 15.0%
!!! TTGTTTTGTATAAAAAAAAG+AGG - chr4_4:81893972-81893991 Msa0708350:CDS 15.0%
!!! TGGTATCGAATTTTTTTTAA+TGG + chr4_4:81890799-81890818 None:intergenic 20.0%
!! ACCTAAATGATAAATTAACT+TGG - chr4_4:81891185-81891204 Msa0708350:CDS 20.0%
!! GTGAAGAATTTAATGATATT+TGG - chr4_4:81891649-81891668 Msa0708350:CDS 20.0%
!! AATTTAATGATATTTGGAGA+TGG - chr4_4:81891655-81891674 Msa0708350:CDS 20.0%
!! TATGAATAGATTAGTGATTA+AGG + chr4_4:81891769-81891788 None:intergenic 20.0%
!! AAAAGTGGAATATATTGTAT+TGG - chr4_4:81892543-81892562 Msa0708350:intron 20.0%
!! AAAGTGGAATATATTGTATT+GGG - chr4_4:81892544-81892563 Msa0708350:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG - chr4_4:81892654-81892673 Msa0708350:intron 20.0%
!!! AACTTTTTACATTAATTGAC+CGG + chr4_4:81892759-81892778 None:intergenic 20.0%
!!! TTATTGGTTTAAATCATTGT+GGG + chr4_4:81892952-81892971 None:intergenic 20.0%
!! GGTCATAATTACTTTATTAA+AGG + chr4_4:81893043-81893062 None:intergenic 20.0%
!! AATTCAGTAAAAACATATCT+TGG - chr4_4:81893562-81893581 Msa0708350:CDS 20.0%
!! GTAATGAAAAGTAATATTGA+TGG - chr4_4:81893616-81893635 Msa0708350:CDS 20.0%
!!! ATTTTCTGTTGGTAATTTTT+TGG - chr4_4:81893798-81893817 Msa0708350:CDS 20.0%
!!! TGTGAATATTGATTAATTTC+TGG - chr4_4:81893869-81893888 Msa0708350:CDS 20.0%
!! TATAGTGAAAGAAATAAAAG+TGG - chr4_4:81893907-81893926 Msa0708350:CDS 20.0%
!! TTTGTATAAAAAAAAGAGGA+TGG - chr4_4:81893976-81893995 Msa0708350:CDS 20.0%
!!! ATTTTTTTTTTTTTGGGTCT+AGG - chr4_4:81894118-81894137 Msa0708350:exon 20.0%
! TAAATATATTTGGAAGAGCT+AGG + chr4_4:81890775-81890794 None:intergenic 25.0%
! GCCAAGTTAATTTATCATTT+AGG + chr4_4:81891189-81891208 None:intergenic 25.0%
!!! ATTTTGACAAGTTTGATGAT+TGG + chr4_4:81891227-81891246 None:intergenic 25.0%
! AATCATCAAACTTGTCAAAA+TGG - chr4_4:81891226-81891245 Msa0708350:CDS 25.0%
! TCATCAAGTACTAAACAAAA+TGG - chr4_4:81891379-81891398 Msa0708350:CDS 25.0%
! TATCAGATAAATTTCCATAC+TGG - chr4_4:81891416-81891435 Msa0708350:CDS 25.0%
! CACTAGAAAATATATCACTT+TGG - chr4_4:81891930-81891949 Msa0708350:intron 25.0%
! TTTATGTACAACTTGTGTTA+AGG - chr4_4:81891983-81892002 Msa0708350:intron 25.0%
! TGTATAAATAAAGGGAAAAG+TGG - chr4_4:81892528-81892547 Msa0708350:intron 25.0%
!!! TTGAAATGAGTCAGTTTTTT+TGG - chr4_4:81892631-81892650 Msa0708350:intron 25.0%
!!! TGAAATGAGTCAGTTTTTTT+GGG - chr4_4:81892632-81892651 Msa0708350:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG - chr4_4:81892653-81892672 Msa0708350:intron 25.0%
! AACAATATTCACTAAAGTTC+TGG - chr4_4:81892716-81892735 Msa0708350:intron 25.0%
!!! CTTATTGGTTTAAATCATTG+TGG + chr4_4:81892953-81892972 None:intergenic 25.0%
! TTTATTACTCACTCTCTTAT+TGG + chr4_4:81892968-81892987 None:intergenic 25.0%
!!! GTAAAGAATTTTTTGCACTA+TGG + chr4_4:81893101-81893120 None:intergenic 25.0%
! AATAATTATTGCAGCTGTTA+TGG - chr4_4:81893168-81893187 Msa0708350:intron 25.0%
! ATAATTATTGCAGCTGTTAT+GGG - chr4_4:81893169-81893188 Msa0708350:intron 25.0%
! TTTGATAAATTCAGAAGGTT+GGG - chr4_4:81893639-81893658 Msa0708350:CDS 25.0%
! TATGGTGAATATATGAATGT+TGG - chr4_4:81893700-81893719 Msa0708350:CDS 25.0%
! TTAGTAATAACATGAAAACC+AGG + chr4_4:81893760-81893779 None:intergenic 25.0%
! TATTGTCATGTAGTATTTGT+TGG - chr4_4:81894035-81894054 Msa0708350:CDS 25.0%
! ATTGTCATGTAGTATTTGTT+GGG - chr4_4:81894036-81894055 Msa0708350:CDS 25.0%
ATGGAAAGAAGAAGAATCTA+GGG + chr4_4:81890996-81891015 None:intergenic 30.0%
TTCTTCTTCTTTCCATGATA+TGG - chr4_4:81891000-81891019 Msa0708350:three_prime_UTR 30.0%
! AGATTGTTTAGAGCTTTATG+AGG - chr4_4:81891123-81891142 Msa0708350:CDS 30.0%
! ACAAGTTTGATGATTGGTAA+TGG + chr4_4:81891221-81891240 None:intergenic 30.0%
TTTAGTGAAGTTGCTTAACA+TGG + chr4_4:81891296-81891315 None:intergenic 30.0%
!! TTGTTTAGTACTTGATGATG+TGG + chr4_4:81891377-81891396 None:intergenic 30.0%
TCAAGTACTAAACAAAATGG+AGG - chr4_4:81891382-81891401 Msa0708350:CDS 30.0%
CAAGTACTAAACAAAATGGA+GGG - chr4_4:81891383-81891402 Msa0708350:CDS 30.0%
AATGATATTTGGAGATGGAA+TGG - chr4_4:81891660-81891679 Msa0708350:CDS 30.0%
ATGATTACCAGTAACAATAG+TGG + chr4_4:81891689-81891708 None:intergenic 30.0%
GGTAATCATAATGCTATAGA+TGG - chr4_4:81891700-81891719 Msa0708350:CDS 30.0%
!! ACAGTTGTTTTCTATATGCA+TGG - chr4_4:81891836-81891855 Msa0708350:intron 30.0%
!! CAGTTGTTTTCTATATGCAT+GGG - chr4_4:81891837-81891856 Msa0708350:intron 30.0%
TTGAGTAACTACTTGTCATA+TGG - chr4_4:81892026-81892045 Msa0708350:intron 30.0%
! CTTTTCTACTTTCTCTCAAA+AGG - chr4_4:81892178-81892197 Msa0708350:intron 30.0%
GATGTAAAAGGTCTAATTGT+TGG + chr4_4:81892337-81892356 None:intergenic 30.0%
!!! TTTTCTGAGACTGATGTAAA+AGG + chr4_4:81892349-81892368 None:intergenic 30.0%
TAAAGTAGGTCAATTATACC+GGG + chr4_4:81892484-81892503 None:intergenic 30.0%
CTAAAGTAGGTCAATTATAC+CGG + chr4_4:81892485-81892504 None:intergenic 30.0%
TCTGTGTGAAAAACTAAAGT+AGG + chr4_4:81892498-81892517 None:intergenic 30.0%
CCTGAACATTTAATAGTATG+TGG + chr4_4:81893064-81893083 None:intergenic 30.0%
CCACATACTATTAAATGTTC+AGG - chr4_4:81893061-81893080 Msa0708350:intron 30.0%
!! GGAACTATTGATTTCATCTT+CGG - chr4_4:81893373-81893392 Msa0708350:CDS 30.0%
! ACAATTCCTGTGTTTTCATT+TGG + chr4_4:81893493-81893512 None:intergenic 30.0%
! GTTTTTACTGAATTCTGAAC+CGG + chr4_4:81893556-81893575 None:intergenic 30.0%
ATGGCAGAAATATTCAAGAA+CGG - chr4_4:81893591-81893610 Msa0708350:CDS 30.0%
GATGGTTTGATAAATTCAGA+AGG - chr4_4:81893634-81893653 Msa0708350:CDS 30.0%
GTTTGATAAATTCAGAAGGT+TGG - chr4_4:81893638-81893657 Msa0708350:CDS 30.0%
GCATTGAGTACACTTTATTA+TGG - chr4_4:81893682-81893701 Msa0708350:CDS 30.0%
TGTTATTACTAATCCTTCTG+AGG - chr4_4:81893768-81893787 Msa0708350:CDS 30.0%
! ACTTGAAATGATGATGACTT+TGG - chr4_4:81893949-81893968 Msa0708350:CDS 30.0%
AAGAGCTAGGTATATAGGAA+TGG + chr4_4:81890762-81890781 None:intergenic 35.0%
TTTGGAAGAGCTAGGTATAT+AGG + chr4_4:81890767-81890786 None:intergenic 35.0%
AGGATATGCAATATTGTGCA+TGG + chr4_4:81890819-81890838 None:intergenic 35.0%
GCACAAACAAGAGAGAAAAA+AGG + chr4_4:81890886-81890905 None:intergenic 35.0%
!! TTTTCTCTCTTGTTTGTGCA+TGG - chr4_4:81890887-81890906 Msa0708350:three_prime_UTR 35.0%
TGTAATGGTTACACACATGA+GGG + chr4_4:81890943-81890962 None:intergenic 35.0%
ATGTAATGGTTACACACATG+AGG + chr4_4:81890944-81890963 None:intergenic 35.0%
CATGTGTGTAACCATTACAT+TGG - chr4_4:81890944-81890963 Msa0708350:three_prime_UTR 35.0%
CATGGAAAGAAGAAGAATCT+AGG + chr4_4:81890997-81891016 None:intergenic 35.0%
TTTCCATGATATGGCTCTTA+AGG - chr4_4:81891009-81891028 Msa0708350:three_prime_UTR 35.0%
TACAAGTTTGTGTGCTTCAA+TGG + chr4_4:81891050-81891069 None:intergenic 35.0%
ACACAAACTTGTATCAACCA+TGG - chr4_4:81891057-81891076 Msa0708350:exon 35.0%
AGTAGTTTAAGTGAGATGGA+AGG + chr4_4:81891267-81891286 None:intergenic 35.0%
TGAAGTTGCTTAACATGGAT+GGG + chr4_4:81891291-81891310 None:intergenic 35.0%
!! TGAAGTTAGAGTGTTGCTAA+TGG + chr4_4:81891338-81891357 None:intergenic 35.0%
!! AAATTTCCATACTGGCTTTC+AGG - chr4_4:81891424-81891443 Msa0708350:CDS 35.0%
!! AATTTCCATACTGGCTTTCA+GGG - chr4_4:81891425-81891444 Msa0708350:CDS 35.0%
! GATATTGTTGTTGCACAAGA+TGG - chr4_4:81891502-81891521 Msa0708350:CDS 35.0%
AAACTATAAGACCATCTCAG+AGG - chr4_4:81891531-81891550 Msa0708350:CDS 35.0%
AACTATAAGACCATCTCAGA+GGG - chr4_4:81891532-81891551 Msa0708350:CDS 35.0%
GTTGTCATTCATGTGAAATC+AGG - chr4_4:81891592-81891611 Msa0708350:CDS 35.0%
!! ACTTTTCGTTCAGCTACTTT+TGG - chr4_4:81891730-81891749 Msa0708350:CDS 35.0%
GGGAGAAATCATATGATCAA+TGG - chr4_4:81891857-81891876 Msa0708350:intron 35.0%
TGCATGTCTCTATCTTAATG+GGG + chr4_4:81891956-81891975 None:intergenic 35.0%
CTGCATGTCTCTATCTTAAT+GGG + chr4_4:81891957-81891976 None:intergenic 35.0%
ACTGCATGTCTCTATCTTAA+TGG + chr4_4:81891958-81891977 None:intergenic 35.0%
AGAAAATGGGTCAGTTATTC+CGG - chr4_4:81892667-81892686 Msa0708350:intron 35.0%
! TACATTAATTGACCGGTTCA+TGG + chr4_4:81892752-81892771 None:intergenic 35.0%
ATCGTTCACAATCAATCATG+AGG - chr4_4:81892816-81892835 Msa0708350:intron 35.0%
TCTAACACTCACTCGTTTAT+TGG - chr4_4:81892871-81892890 Msa0708350:intron 35.0%
AAAATTGAACCTCCACAAAG+TGG + chr4_4:81892918-81892937 None:intergenic 35.0%
ACAGATCCAAATGAAAACAC+AGG - chr4_4:81893484-81893503 Msa0708350:CDS 35.0%
! AATCATTTGCTGCGGTTATA+CGG + chr4_4:81893528-81893547 None:intergenic 35.0%
AAACATATCTTGGTAGACCA+TGG - chr4_4:81893572-81893591 Msa0708350:CDS 35.0%
! GTTCTTGAATATTTCTGCCA+TGG + chr4_4:81893592-81893611 None:intergenic 35.0%
GGTGAATATATGAATGTTGG+TGG - chr4_4:81893703-81893722 Msa0708350:CDS 35.0%
! GAATATATGAATGTTGGTGG+TGG - chr4_4:81893706-81893725 Msa0708350:CDS 35.0%
ATACTGGTGGAAGAGTTAAT+TGG - chr4_4:81893734-81893753 Msa0708350:CDS 35.0%
GAAAATTTAACAGCCTCAGA+AGG + chr4_4:81893784-81893803 None:intergenic 35.0%
! GAGGCTGTTAAATTTTCTGT+TGG - chr4_4:81893787-81893806 Msa0708350:CDS 35.0%
!!! TCTGTTGGTAATTTTTTGGC+TGG - chr4_4:81893802-81893821 Msa0708350:CDS 35.0%
!!! ATTTTTTGGCTGGTGATTCT+TGG - chr4_4:81893812-81893831 Msa0708350:CDS 35.0%
ATTCACAAACCTGCATCAAA+CGG + chr4_4:81893856-81893875 None:intergenic 35.0%
!! AAAAGTGGTTGATGATTCAG+TGG - chr4_4:81893922-81893941 Msa0708350:CDS 35.0%
!! AAAGTGGTTGATGATTCAGT+GGG - chr4_4:81893923-81893942 Msa0708350:CDS 35.0%
TCTTGTGTAACCTTTGTACA+AGG + chr4_4:81894068-81894087 None:intergenic 35.0%
GGTATATAGGAATGGATGAG+TGG + chr4_4:81890754-81890773 None:intergenic 40.0%
TGACCTTAAGAGCCATATCA+TGG + chr4_4:81891015-81891034 None:intergenic 40.0%
! ACAATCTTCCCATGCTGATT+TGG + chr4_4:81891110-81891129 None:intergenic 40.0%
GGGAAGTAGTTTAAGTGAGA+TGG + chr4_4:81891271-81891290 None:intergenic 40.0%
GTGAAGTTGCTTAACATGGA+TGG + chr4_4:81891292-81891311 None:intergenic 40.0%
!! AGTACTTGATGATGTGGATG+AGG + chr4_4:81891371-81891390 None:intergenic 40.0%
CTAAACAAAATGGAGGGAGA+AGG - chr4_4:81891389-81891408 Msa0708350:CDS 40.0%
GCAACAACAATATCAGCTCT+TGG + chr4_4:81891496-81891515 None:intergenic 40.0%
ATGGACTCCACTATTGTTAC+TGG - chr4_4:81891679-81891698 Msa0708350:CDS 40.0%
AGGTCTAATTGTTGGTCGTA+TGG + chr4_4:81892329-81892348 None:intergenic 40.0%
GAAAATGGGTCAGTTATTCC+GGG - chr4_4:81892668-81892687 Msa0708350:intron 40.0%
GGAGTTTAATCTCCATGAAC+CGG - chr4_4:81892737-81892756 Msa0708350:intron 40.0%
! GGACATGACCTTTGAGAATA+CGG - chr4_4:81893210-81893229 Msa0708350:CDS 40.0%
!!! AGCTACTGCTTGGTGTTTTT+GGG + chr4_4:81893242-81893261 None:intergenic 40.0%
! CGTAAGCATATAAGGTGTCT+TGG + chr4_4:81893318-81893337 None:intergenic 40.0%
!! CGAAGATGTTGCAGTTTTGA+AGG + chr4_4:81893414-81893433 None:intergenic 40.0%
! TGTTCTTCCTTGTGCTGTTA+CGG + chr4_4:81893467-81893486 None:intergenic 40.0%
! CGGTTTCAAATCATTTGCTG+CGG + chr4_4:81893536-81893555 None:intergenic 40.0%
! CGCAGCAAATGATTTGAAAC+CGG - chr4_4:81893534-81893553 Msa0708350:CDS 40.0%
CTGGTGATTCTTGGATTTCA+GGG - chr4_4:81893821-81893840 Msa0708350:CDS 40.0%
! TGGTGGTGACAATTGAAGAT+GGG - chr4_4:81894007-81894026 Msa0708350:CDS 40.0%
! GTTACACACATGAGGGTATG+GGG + chr4_4:81890936-81890955 None:intergenic 45.0%
! GGTTACACACATGAGGGTAT+GGG + chr4_4:81890937-81890956 None:intergenic 45.0%
TGGTTACACACATGAGGGTA+TGG + chr4_4:81890938-81890957 None:intergenic 45.0%
!! TGGTGGTGGTTCCAATGTAA+TGG + chr4_4:81890958-81890977 None:intergenic 45.0%
! ATCTAGGGTAGACAGTGTGT+TGG + chr4_4:81890981-81891000 None:intergenic 45.0%
TATGGCTCTTAAGGTCACCA+TGG - chr4_4:81891018-81891037 Msa0708350:three_prime_UTR 45.0%
GTTTGTGTGCTTCAATGGCT+TGG + chr4_4:81891045-81891064 None:intergenic 45.0%
GATGATGTGGATGAGGATGA+TGG + chr4_4:81891364-81891383 None:intergenic 45.0%
GTCAGATAGAAAGCTGCTTC+AGG - chr4_4:81891447-81891466 Msa0708350:CDS 45.0%
! ACAATATCAGCTCTTGGTGC+TGG + chr4_4:81891490-81891509 None:intergenic 45.0%
GTTGTTGCACAAGATGGAAG+TGG - chr4_4:81891508-81891527 Msa0708350:CDS 45.0%
TTGCAGCTGCTAAGCTTAGT+GGG - chr4_4:81891560-81891579 Msa0708350:CDS 45.0%
ATATATTACTCCCTCCGTCC+CGG - chr4_4:81892463-81892482 Msa0708350:intron 45.0%
GTAGGTCAATTATACCGGGA+CGG + chr4_4:81892480-81892499 None:intergenic 45.0%
! TTTTATTACTCCCTCCGTCC+CGG + chr4_4:81892689-81892708 None:intergenic 45.0%
ATGTATGTCCCACCACTTTG+TGG - chr4_4:81892903-81892922 Msa0708350:intron 45.0%
TTGAACCTCCACAAAGTGGT+GGG + chr4_4:81892914-81892933 None:intergenic 45.0%
ATTGAACCTCCACAAAGTGG+TGG + chr4_4:81892915-81892934 None:intergenic 45.0%
ATTGCAGCTGTTATGGGAGA+TGG - chr4_4:81893175-81893194 Msa0708350:intron 45.0%
GGGAGATGGTTTCATAGCTA+AGG - chr4_4:81893189-81893208 Msa0708350:CDS 45.0%
! ATGACCTTTGAGAATACGGC+AGG - chr4_4:81893214-81893233 Msa0708350:CDS 45.0%
!!! GCTACTGCTTGGTGTTTTTG+GGG + chr4_4:81893241-81893260 None:intergenic 45.0%
!!! GAGCTACTGCTTGGTGTTTT+TGG + chr4_4:81893243-81893262 None:intergenic 45.0%
ACGGTAGAAAACGGAGTGAT+CGG + chr4_4:81893278-81893297 None:intergenic 45.0%
! GAAGGCACAACGGTAGAAAA+CGG + chr4_4:81893287-81893306 None:intergenic 45.0%
TTCTACCGTTGTGCCTTCAA+AGG - chr4_4:81893289-81893308 Msa0708350:CDS 45.0%
! GTGTTCTGTTGGTTGCTCAT+TGG + chr4_4:81893445-81893464 None:intergenic 45.0%
AATGCAAAACCACCCATCCA+TGG + chr4_4:81893667-81893686 None:intergenic 45.0%
GCTGGTGATTCTTGGATTTC+AGG - chr4_4:81893820-81893839 Msa0708350:CDS 45.0%
GATTCTTGGATTTCAGGGAG+TGG - chr4_4:81893826-81893845 Msa0708350:CDS 45.0%
AAAAGAGGATGGTGAAAGCG+TGG - chr4_4:81893987-81894006 Msa0708350:CDS 45.0%
! GTGGTGGTGACAATTGAAGA+TGG - chr4_4:81894006-81894025 Msa0708350:CDS 45.0%
GGTGGTGACAATTGAAGATG+GGG - chr4_4:81894008-81894027 Msa0708350:CDS 45.0%
TGGGTGTTTGCCTTGTACAA+AGG - chr4_4:81894055-81894074 Msa0708350:CDS 45.0%
!! CAAAAAAAAAAAAATAATAA+TGG + chr4_4:81894113-81894132 None:intergenic 5.0%
!!! CATTATTATTTTTTTTTTTT+TGG - chr4_4:81894111-81894130 Msa0708350:exon 5.0%
TAGGGTAGACAGTGTGTTGG+TGG + chr4_4:81890978-81890997 None:intergenic 50.0%
!! TGCTTCAATGGCTTGGTCCA+TGG + chr4_4:81891038-81891057 None:intergenic 50.0%
ACAAGCGTGCCAAATCAGCA+TGG - chr4_4:81891098-81891117 Msa0708350:CDS 50.0%
CAAGCGTGCCAAATCAGCAT+GGG - chr4_4:81891099-81891118 Msa0708350:CDS 50.0%
TCTGACCCTGAAAGCCAGTA+TGG + chr4_4:81891433-81891452 None:intergenic 50.0%
! TGCAACAACACCCTCTGAGA+TGG + chr4_4:81891545-81891564 None:intergenic 50.0%
GTTGCAGCTGCTAAGCTTAG+TGG - chr4_4:81891559-81891578 Msa0708350:CDS 50.0%
GCTGCTAAGCTTAGTGGGAA+AGG - chr4_4:81891565-81891584 Msa0708350:CDS 50.0%
GTCAATTATACCGGGACGGA+GGG + chr4_4:81892476-81892495 None:intergenic 50.0%
ATGGGTCAGTTATTCCGGGA+CGG - chr4_4:81892672-81892691 Msa0708350:intron 50.0%
TATGTCCCACCACTTTGTGG+AGG - chr4_4:81892906-81892925 Msa0708350:intron 50.0%
! GGTAGCCTTTGAAGGCACAA+CGG + chr4_4:81893297-81893316 None:intergenic 50.0%
! GGTGTCTTGGTAGCCTTTGA+AGG + chr4_4:81893305-81893324 None:intergenic 50.0%
GCGGTTGGCGTAAGCATATA+AGG + chr4_4:81893326-81893345 None:intergenic 50.0%
TGTTGCAGTCGCGGTAGAAT+TGG + chr4_4:81893348-81893367 None:intergenic 50.0%
TACCGCGACTGCAACATCTA+CGG - chr4_4:81893352-81893371 Msa0708350:CDS 50.0%
TTCCGTAGATGTTGCAGTCG+CGG + chr4_4:81893357-81893376 None:intergenic 50.0%
! GTGCTGTTACGGTGTTCTGT+TGG + chr4_4:81893456-81893475 None:intergenic 50.0%
CAGAACACCGTAACAGCACA+AGG - chr4_4:81893457-81893476 Msa0708350:CDS 50.0%
ATTCAGAAGGTTGGGCTCCA+TGG - chr4_4:81893647-81893666 Msa0708350:CDS 50.0%
!! GTTGGTGGTGGTGCTAATAC+TGG - chr4_4:81893718-81893737 Msa0708350:CDS 50.0%
GGTGGAAGAGTTAATTGGCC+TGG - chr4_4:81893739-81893758 Msa0708350:CDS 50.0%
!! AGACAGTGTGTTGGTGGTGG+TGG + chr4_4:81890972-81890991 None:intergenic 55.0%
! GGTAGACAGTGTGTTGGTGG+TGG + chr4_4:81890975-81890994 None:intergenic 55.0%
AAGCTGCTTCAGGCAACACC+AGG - chr4_4:81891457-81891476 Msa0708350:CDS 55.0%
AGCTGCTTCAGGCAACACCA+GGG - chr4_4:81891458-81891477 Msa0708350:CDS 55.0%
TTCAGGCAACACCAGGGTCA+GGG - chr4_4:81891464-81891483 Msa0708350:CDS 55.0%
GGTCAATTATACCGGGACGG+AGG + chr4_4:81892477-81892496 None:intergenic 55.0%
GTCAGTTATTCCGGGACGGA+GGG - chr4_4:81892676-81892695 Msa0708350:intron 55.0%
GGGTCCTGCCGTATTCTCAA+AGG + chr4_4:81893221-81893240 None:intergenic 55.0%
CAAGCAGTAGCTCTCCGTTC+CGG - chr4_4:81893250-81893269 Msa0708350:CDS 55.0%
TGCAGTCGCGGTAGAATTGG+CGG + chr4_4:81893345-81893364 None:intergenic 55.0%
AGAAGGTTGGGCTCCATGGA+TGG - chr4_4:81893651-81893670 Msa0708350:CDS 55.0%
GAAGGTTGGGCTCCATGGAT+GGG - chr4_4:81893652-81893671 Msa0708350:CDS 55.0%
! GGTGGTGGTGCTAATACTGG+TGG - chr4_4:81893721-81893740 Msa0708350:CDS 55.0%
!! AGTGGTGTGCCGTTTGATGC+AGG - chr4_4:81893844-81893863 Msa0708350:CDS 55.0%
! AGAGGATGGTGAAAGCGTGG+TGG - chr4_4:81893990-81894009 Msa0708350:CDS 55.0%
CTTCAGGCAACACCAGGGTC+AGG - chr4_4:81891463-81891482 Msa0708350:CDS 60.0%
GGTCAGTTATTCCGGGACGG+AGG - chr4_4:81892675-81892694 Msa0708350:intron 60.0%
CGGAACGGAGAGCTACTGCT+TGG + chr4_4:81893252-81893271 None:intergenic 60.0%
GAAAACGGAGTGATCGGCGC+CGG + chr4_4:81893272-81893291 None:intergenic 60.0%
GTCGCGGTAGAATTGGCGGT+TGG + chr4_4:81893341-81893360 None:intergenic 60.0%
GGTTGGGCTCCATGGATGGG+TGG - chr4_4:81893655-81893674 Msa0708350:CDS 65.0%
!! CTTGGTGCTGGCCCTGACCC+TGG + chr4_4:81891478-81891497 None:intergenic 70.0%
! CGGAGTGATCGGCGCCGGAA+CGG + chr4_4:81893267-81893286 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 81890744 81894196 81890744 ID=Msa0708350;Name=Msa0708350
chr4_4 mRNA 81890744 81894196 81890744 ID=Msa0708350-mRNA-1;Parent=Msa0708350;Name=Msa0708350-mRNA-1;_AED=0.02;_eAED=0.02;_QI=74|1|1|1|1|1|2|323|541
chr4_4 exon 81890744 81891758 81890744 ID=Msa0708350-mRNA-1:exon:26939;Parent=Msa0708350-mRNA-1
chr4_4 exon 81893189 81894196 81893189 ID=Msa0708350-mRNA-1:exon:26938;Parent=Msa0708350-mRNA-1
chr4_4 five_prime_UTR 81894123 81894196 81894123 ID=Msa0708350-mRNA-1:five_prime_utr;Parent=Msa0708350-mRNA-1
chr4_4 CDS 81893189 81894122 81893189 ID=Msa0708350-mRNA-1:cds;Parent=Msa0708350-mRNA-1
chr4_4 CDS 81891067 81891758 81891067 ID=Msa0708350-mRNA-1:cds;Parent=Msa0708350-mRNA-1
chr4_4 three_prime_UTR 81890744 81891066 81890744 ID=Msa0708350-mRNA-1:three_prime_utr;Parent=Msa0708350-mRNA-1
Gene Sequence

>Msa0708350

ATGCACAATATTGCATATCCTAATATGAATAAACTCATATTCAACTCTTGCATTGCATACTTTCTCCTTTTTTCTCTCTTGTTTGTGCATGGCAAAGAGTTATCATGCAATCAAACCCCATACCCTCATGTGTGTAACCATTACATTGGAACCACCACCACCAACACACTGTCTACCCTAGATTCTTCTTCTTTCCATGATATGGCTCTTAAGGTCACCATGGACCAAGCCATTGAAGCACACAAACTTGTATCAACCATGGAATTAAATAATTTCAAAGACAAGCGTGCCAAATCAGCATGGGAAGATTGTTTAGAGCTTTATGAGGATACAATTTATCAACTCAAACGCTCAATAAACTCAAACAACCTAAATGATAAATTAACTTGGCAAAGTGCTTCCATTACCAATCATCAAACTTGTCAAAATGGTTTCATTGATTTCAACCTTCCATCTCACTTAAACTACTTCCCATCCATGTTAAGCAACTTCACTAAATTGCTTAGCAATTCTTTGTCCATTAGCAACACTCTAACTTCATCACCATCATCCTCATCCACATCATCAAGTACTAAACAAAATGGAGGGAGAAGGTTGTTATCAGATAAATTTCCATACTGGCTTTCAGGGTCAGATAGAAAGCTGCTTCAGGCAACACCAGGGTCAGGGCCAGCACCAAGAGCTGATATTGTTGTTGCACAAGATGGAAGTGGAAACTATAAGACCATCTCAGAGGGTGTTGTTGCAGCTGCTAAGCTTAGTGGGAAAGGAAGAGTTGTCATTCATGTGAAATCAGGTGTTTATAGAGAGAATGTTGATATCAAGAAGACAGTGAAGAATTTAATGATATTTGGAGATGGAATGGACTCCACTATTGTTACTGGTAATCATAATGCTATAGATGGTTCTACTACTTTTCGTTCAGCTACTTTTGCTGTTATGGGAGATGGTTTCATAGCTAAGGACATGACCTTTGAGAATACGGCAGGACCCCAAAAACACCAAGCAGTAGCTCTCCGTTCCGGCGCCGATCACTCCGTTTTCTACCGTTGTGCCTTCAAAGGCTACCAAGACACCTTATATGCTTACGCCAACCGCCAATTCTACCGCGACTGCAACATCTACGGAACTATTGATTTCATCTTCGGCAACGCAGTTACTGTCCTTCAAAACTGCAACATCTTCGTTAGAAAACCAATGAGCAACCAACAGAACACCGTAACAGCACAAGGAAGAACAGATCCAAATGAAAACACAGGAATTGTAATACATAACTGCCGTATAACCGCAGCAAATGATTTGAAACCGGTTCAGAATTCAGTAAAAACATATCTTGGTAGACCATGGCAGAAATATTCAAGAACGGTTGTAATGAAAAGTAATATTGATGGTTTGATAAATTCAGAAGGTTGGGCTCCATGGATGGGTGGTTTTGCATTGAGTACACTTTATTATGGTGAATATATGAATGTTGGTGGTGGTGCTAATACTGGTGGAAGAGTTAATTGGCCTGGTTTTCATGTTATTACTAATCCTTCTGAGGCTGTTAAATTTTCTGTTGGTAATTTTTTGGCTGGTGATTCTTGGATTTCAGGGAGTGGTGTGCCGTTTGATGCAGGTTTGTGA
Protein sequence

>Msa0708350

MHNIAYPNMNKLIFNSCIAYFLLFSLLFVHGKELSCNQTPYPHVCNHYIGTTTTNTLSTLDSSSFHDMALKVTMDQAIEAHKLVSTMELNNFKDKRAKSAWEDCLELYEDTIYQLKRSINSNNLNDKLTWQSASITNHQTCQNGFIDFNLPSHLNYFPSMLSNFTKLLSNSLSISNTLTSSPSSSSTSSSTKQNGGRRLLSDKFPYWLSGSDRKLLQATPGSGPAPRADIVVAQDGSGNYKTISEGVVAAAKLSGKGRVVIHVKSGVYRENVDIKKTVKNLMIFGDGMDSTIVTGNHNAIDGSTTFRSATFAVMGDGFIAKDMTFENTAGPQKHQAVALRSGADHSVFYRCAFKGYQDTLYAYANRQFYRDCNIYGTIDFIFGNAVTVLQNCNIFVRKPMSNQQNTVTAQGRTDPNENTGIVIHNCRITAANDLKPVQNSVKTYLGRPWQKYSRTVVMKSNIDGLINSEGWAPWMGGFALSTLYYGEYMNVGGGANTGGRVNWPGFHVITNPSEAVKFSVGNFLAGDSWISGSGVPFDAGL*