AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1353940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1353940 MtrunA17_Chr8g0391311 93.542 542 33 2 1 541 1 541 0.0 1041
Msa1353940 MtrunA17_Chr8g0391291 92.989 542 34 3 1 541 1 539 0.0 1030
Msa1353940 MtrunA17_Chr8g0391281 91.026 312 24 3 1 311 1 309 0.0 557
Msa1353940 MtrunA17_Chr4g0011771 50.838 537 227 11 13 541 6 513 0.0 530
Msa1353940 MtrunA17_Chr4g0011791 50.375 534 224 13 18 541 7 509 5.31e-179 514
Msa1353940 MtrunA17_Chr3g0079341 47.082 514 242 7 36 541 36 527 2.47e-161 469
Msa1353940 MtrunA17_Chr4g0018371 44.942 514 261 6 36 541 40 539 2.57e-160 467
Msa1353940 MtrunA17_Chr3g0090091 47.104 518 237 10 36 541 36 528 7.82e-160 466
Msa1353940 MtrunA17_Chr3g0079361 46.693 514 244 6 36 541 36 527 1.91e-159 464
Msa1353940 MtrunA17_Chr3g0079351 46.887 514 243 8 36 541 36 527 7.74e-159 463
Msa1353940 MtrunA17_Chr4g0011751 50.105 475 203 8 74 541 42 489 7.64e-158 459
Msa1353940 MtrunA17_Chr7g0269541 45.229 524 259 7 29 537 42 552 3.26e-154 452
Msa1353940 MtrunA17_Chr7g0233771 45.920 527 244 11 37 541 47 554 8.65e-153 448
Msa1353940 MtrunA17_Chr8g0345281 44.381 525 268 6 35 541 71 589 1.79e-149 441
Msa1353940 MtrunA17_Chr7g0233801 43.762 521 253 10 42 541 52 553 7.81e-142 420
Msa1353940 MtrunA17_Chr1g0147051 42.214 533 272 10 35 541 85 607 1.62e-141 421
Msa1353940 MtrunA17_Chr3g0126771 42.430 535 262 11 35 541 81 597 2.16e-141 421
Msa1353940 MtrunA17_Chr8g0354901 50.321 467 199 9 88 541 135 581 3.18e-141 420
Msa1353940 MtrunA17_Chr2g0302021 46.849 476 219 7 79 541 71 525 4.97e-141 417
Msa1353940 MtrunA17_Chr7g0269531 45.047 535 251 11 33 537 40 561 3.53e-140 417
Msa1353940 MtrunA17_Chr7g0233831 45.714 490 237 8 57 541 90 555 3.56e-140 416
Msa1353940 MtrunA17_Chr1g0192331 45.698 523 243 14 35 541 31 528 2.50e-139 413
Msa1353940 MtrunA17_Chr4g0069841 45.720 479 215 8 81 541 65 516 2.65e-137 407
Msa1353940 MtrunA17_Chr7g0272141 45.051 495 234 10 63 541 70 542 4.96e-137 408
Msa1353940 MtrunA17_Chr7g0269521 50.698 430 190 8 129 541 138 562 1.52e-134 402
Msa1353940 MtrunA17_Chr7g0269511 42.750 531 273 8 35 541 41 564 4.41e-134 401
Msa1353940 MtrunA17_Chr3g0080391 46.753 462 215 8 92 541 132 574 2.06e-133 399
Msa1353940 MtrunA17_Chr4g0069891 41.966 529 270 10 33 541 82 593 1.88e-132 398
Msa1353940 MtrunA17_Chr1g0195301 42.473 558 277 12 18 541 8 555 1.82e-131 394
Msa1353940 MtrunA17_Chr1g0195321 42.473 558 277 12 18 541 8 555 1.82e-131 394
Msa1353940 MtrunA17_Chr1g0195331 42.832 558 275 12 18 541 8 555 1.94e-131 394
Msa1353940 MtrunA17_Chr4g0069881 42.226 521 257 13 36 541 44 535 1.02e-130 391
Msa1353940 MtrunA17_Chr8g0345211 43.238 525 256 13 36 541 81 582 8.16e-130 390
Msa1353940 MtrunA17_Chr8g0354911 41.652 557 254 13 35 541 77 612 1.82e-129 391
Msa1353940 MtrunA17_Chr4g0069861 43.552 473 236 6 81 541 64 517 2.07e-129 387
Msa1353940 MtrunA17_Chr1g0175661 42.495 553 277 13 21 541 15 558 4.01e-129 388
Msa1353940 MtrunA17_Chr1g0195351 42.115 558 277 13 18 541 8 553 2.80e-126 380
Msa1353940 MtrunA17_Chr1g0194091 40.147 543 290 12 27 541 34 569 3.34e-123 373
Msa1353940 MtrunA17_Chr1g0194081 38.346 532 303 11 30 541 37 563 2.50e-119 363
Msa1353940 MtrunA17_Chr1g0195311 61.597 263 100 1 279 541 53 314 4.75e-119 353
Msa1353940 MtrunA17_Chr3g0080381 43.537 441 221 7 124 541 193 628 3.49e-117 360
Msa1353940 MtrunA17_Chr1g0192311 39.114 519 285 14 36 537 62 566 2.79e-111 343
Msa1353940 MtrunA17_Chr1g0192281 39.114 519 285 14 36 537 62 566 2.79e-111 343
Msa1353940 MtrunA17_Chr1g0192321 37.569 543 281 14 36 540 60 582 2.49e-110 340
Msa1353940 MtrunA17_Chr7g0272151 37.757 535 288 12 36 540 63 582 4.95e-110 340
Msa1353940 MtrunA17_Chr1g0192301 37.765 519 292 12 36 537 67 571 1.14e-109 339
Msa1353940 MtrunA17_Chr4g0000121 37.380 519 294 12 36 537 49 553 1.12e-108 336
Msa1353940 MtrunA17_Chr4g0067611 38.772 521 291 12 36 540 63 571 1.35e-108 336
Msa1353940 MtrunA17_Chr4g0067601 38.403 526 292 12 33 540 60 571 3.10e-108 335
Msa1353940 MtrunA17_Chr7g0270661 39.308 491 265 9 75 536 83 569 3.62e-104 324
Msa1353940 MtrunA17_Chr7g0270671 39.308 491 265 9 75 536 83 569 3.62e-104 324
Msa1353940 MtrunA17_Chr2g0301931 53.521 284 127 3 258 541 14 292 6.68e-104 314
Msa1353940 MtrunA17_Chr7g0234711 62.281 228 85 1 315 541 42 269 5.43e-103 310
Msa1353940 MtrunA17_Chr2g0295531 35.687 524 293 12 33 537 44 542 4.06e-102 320
Msa1353940 MtrunA17_Chr7g0270651 36.059 538 309 10 33 541 43 574 5.87e-102 318
Msa1353940 MtrunA17_Chr7g0270691 34.674 522 301 9 36 541 52 549 3.57e-99 310
Msa1353940 MtrunA17_Chr2g0302001 53.150 254 118 1 288 541 185 437 1.08e-98 305
Msa1353940 MtrunA17_Chr2g0295551 35.917 529 296 11 33 540 57 563 8.50e-98 307
Msa1353940 MtrunA17_Chr7g0270631 38.144 485 271 8 75 532 71 553 4.07e-93 307
Msa1353940 MtrunA17_Chr7g0270631 37.500 480 268 9 75 524 619 1096 1.60e-82 278
Msa1353940 MtrunA17_Chr8g0391261 92.806 139 10 0 403 541 28 166 1.61e-91 277
Msa1353940 MtrunA17_Chr8g0384121 34.733 524 301 13 31 533 53 556 4.43e-91 290
Msa1353940 MtrunA17_Chr3g0129921 34.879 539 284 16 33 534 49 557 1.40e-89 286
Msa1353940 MtrunA17_Chr2g0295541 33.097 565 294 14 33 540 57 594 1.70e-88 284
Msa1353940 MtrunA17_Chr5g0437831 41.520 342 193 6 202 541 47 383 1.37e-82 262
Msa1353940 MtrunA17_Chr8g0380581 39.169 337 195 4 203 537 44 372 4.45e-82 261
Msa1353940 MtrunA17_Chr7g0268241 39.936 313 175 5 230 541 50 350 3.70e-76 244
Msa1353940 MtrunA17_Chr2g0334111 38.217 314 179 5 229 541 24 323 6.01e-75 240
Msa1353940 MtrunA17_Chr7g0271381 31.716 536 293 17 36 541 64 556 1.69e-68 230
Msa1353940 MtrunA17_Chr6g0487101 39.085 284 147 7 258 530 112 380 1.73e-50 177
Msa1353940 MtrunA17_Chr8g0389771 38.811 286 147 7 258 530 107 377 9.35e-50 176
Msa1353940 MtrunA17_Chr1g0194311 32.733 333 193 8 208 530 57 368 1.58e-49 175
Msa1353940 MtrunA17_Chr6g0453551 33.770 305 177 6 230 524 6 295 6.92e-49 171
Msa1353940 MtrunA17_Chr2g0299431 43.388 242 122 6 203 439 30 261 2.02e-48 169
Msa1353940 MtrunA17_Chr6g0466951 34.722 288 164 6 221 503 60 328 3.64e-48 171
Msa1353940 MtrunA17_Chr7g0258341 31.443 388 232 7 154 531 25 388 2.05e-47 169
Msa1353940 MtrunA17_Chr7g0217941 32.099 324 189 7 232 539 8 316 4.97e-46 164
Msa1353940 MtrunA17_Chr6g0466961 36.140 285 154 7 226 503 36 299 5.62e-46 164
Msa1353940 MtrunA17_Chr1g0161731 32.384 281 161 5 261 534 79 337 9.54e-43 155
Msa1353940 MtrunA17_Chr7g0234731 34.043 282 157 6 36 310 55 314 2.34e-42 154
Msa1353940 MtrunA17_Chr4g0070051 31.833 311 181 12 230 530 54 343 5.81e-38 142
Msa1353940 MtrunA17_Chr5g0446301 32.573 307 179 7 236 530 67 357 9.79e-38 142
Msa1353940 MtrunA17_Chr5g0440871 31.596 307 185 7 227 531 36 319 6.02e-36 137
Msa1353940 MtrunA17_Chr7g0265201 29.085 306 189 6 232 530 101 385 1.04e-34 134
Msa1353940 MtrunA17_Chr5g0437821 46.212 132 70 1 344 474 4 135 1.08e-34 127
Msa1353940 MtrunA17_Chr5g0399221 29.483 329 200 8 223 539 56 364 3.18e-34 132
Msa1353940 MtrunA17_Chr2g0294321 29.231 325 204 7 212 530 42 346 5.40e-34 132
Msa1353940 MtrunA17_Chr8g0351871 29.114 316 195 8 225 531 28 323 2.22e-31 124
Msa1353940 MtrunA17_Chr4g0037751 29.964 277 175 6 230 500 71 334 2.20e-30 122
Msa1353940 MtrunA17_Chr6g0459781 38.333 180 95 5 230 396 13 189 7.23e-30 116
Msa1353940 MtrunA17_Chr2g0295851 28.221 326 197 10 212 530 41 336 7.90e-29 117
Msa1353940 MtrunA17_Chr5g0420831 29.474 285 170 8 230 502 71 336 1.86e-28 116
Msa1353940 MtrunA17_Chr4g0025841 30.204 245 153 5 230 474 37 263 2.37e-26 108
Msa1353940 MtrunA17_Chr5g0440901 34.868 152 89 2 323 474 1 142 1.20e-23 97.1
Msa1353940 MtrunA17_Chr7g0265161 30.939 181 92 4 296 471 5 157 2.80e-21 93.2
Msa1353940 MtrunA17_Chr2g0314691 28.788 198 126 4 334 530 18 201 3.30e-20 89.0
Msa1353940 MtrunA17_Chr6g0460141 37.681 138 78 5 261 390 7 144 1.00e-18 83.2
Msa1353940 MtrunA17_Chr5g0440891 33.333 156 96 3 230 385 48 195 2.99e-17 80.5
Msa1353940 MtrunA17_Chr4g0073911 27.273 176 118 3 261 429 38 210 7.04e-16 77.4
Msa1353940 MtrunA17_Chr6g0459791 36.800 125 66 4 407 530 4 116 3.62e-15 72.0
Msa1353940 MtrunA17_Chr4g0018251 28.313 166 103 5 27 181 35 195 4.54e-11 62.4
Msa1353940 MtrunA17_Chr4g0018241 28.313 166 103 5 27 181 35 195 5.33e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1353940 AT1G23200.1 52.603 557 225 13 11 541 11 554 0.0 550
Msa1353940 AT2G45220.1 50.392 510 217 11 36 541 34 511 7.39e-174 501
Msa1353940 AT5G51490.1 43.762 521 256 9 36 541 38 536 6.31e-149 438
Msa1353940 AT3G49220.1 45.019 522 257 13 35 541 90 596 5.60e-148 438
Msa1353940 AT3G60730.1 45.344 494 240 7 70 541 34 519 1.89e-147 434
Msa1353940 AT5G51500.1 43.846 520 257 10 36 541 42 540 2.21e-147 434
Msa1353940 AT3G49220.3 45.019 522 257 13 35 541 135 641 3.02e-147 437
Msa1353940 AT5G53370.1 44.208 518 268 10 35 541 78 585 1.56e-145 431
Msa1353940 AT3G47400.1 43.212 523 264 8 36 541 88 594 2.84e-145 431
Msa1353940 AT5G53370.2 44.208 518 268 10 35 541 114 621 3.74e-145 432
Msa1353940 AT3G14310.1 43.623 541 261 9 35 541 62 592 2.67e-144 428
Msa1353940 AT1G11580.1 44.246 504 260 6 45 541 68 557 6.74e-142 421
Msa1353940 AT1G11580.2 44.246 504 260 6 45 541 89 578 1.53e-141 421
Msa1353940 AT1G53830.1 42.509 534 267 8 36 541 66 587 1.00e-138 414
Msa1353940 AT3G59010.1 46.170 483 227 11 69 541 69 528 5.48e-137 407
Msa1353940 AT3G43270.1 45.979 485 241 6 69 541 50 525 1.93e-135 404
Msa1353940 AT4G00190.1 42.775 519 247 11 28 541 1 474 1.87e-133 396
Msa1353940 AT2G26440.1 42.360 517 277 8 36 541 41 547 1.97e-133 399
Msa1353940 AT4G02330.1 42.004 569 279 12 18 541 9 571 1.10e-132 398
Msa1353940 AT2G26450.1 41.779 517 272 9 36 541 113 611 1.58e-131 396
Msa1353940 AT5G27870.1 41.586 517 276 11 36 541 61 562 7.03e-131 399
Msa1353940 AT3G05620.1 41.935 527 261 9 35 541 40 541 8.90e-131 392
Msa1353940 AT4G02320.1 45.175 456 230 5 96 541 73 518 1.01e-130 391
Msa1353940 AT2G43050.1 45.618 445 208 7 103 541 100 516 1.37e-129 388
Msa1353940 AT4G02300.1 43.818 461 223 7 98 541 91 532 1.77e-129 388
Msa1353940 AT3G05610.1 41.154 520 276 11 36 541 62 565 4.60e-129 392
Msa1353940 AT5G04960.1 41.617 507 277 9 36 539 72 562 5.25e-129 388
Msa1353940 AT4G33220.1 42.585 472 237 6 94 541 62 523 7.75e-129 386
Msa1353940 AT3G10710.1 41.942 515 263 9 36 541 74 561 7.04e-126 380
Msa1353940 AT1G02810.1 55.205 317 138 3 228 541 262 577 2.29e-123 374
Msa1353940 AT5G04970.1 40.183 545 279 13 34 541 86 620 3.19e-123 375
Msa1353940 AT4G33220.2 50.442 339 162 2 203 541 70 402 1.17e-122 366
Msa1353940 AT3G10720.2 38.961 539 292 9 35 541 82 615 1.18e-122 374
Msa1353940 AT5G49180.1 40.535 523 281 13 33 537 56 566 1.31e-122 372
Msa1353940 AT2G47550.1 45.114 481 231 10 88 541 84 558 3.24e-122 370
Msa1353940 AT4G33230.1 40.192 520 285 10 36 541 99 606 3.99e-122 372
Msa1353940 AT3G06830.1 39.771 523 285 13 36 539 55 566 5.43e-122 370
Msa1353940 AT5G20860.2 39.469 527 271 12 36 541 39 538 5.55e-121 367
Msa1353940 AT1G53840.1 39.469 527 270 14 36 535 77 581 2.38e-120 367
Msa1353940 AT3G14300.1 40.000 525 270 14 36 536 463 966 2.37e-113 359
Msa1353940 AT3G10720.1 56.538 260 112 1 282 541 1 259 3.97e-108 324
Msa1353940 AT4G03930.1 35.926 540 304 11 20 541 9 524 1.53e-105 327
Msa1353940 AT3G27980.1 47.478 337 169 3 205 541 169 497 3.42e-103 320
Msa1353940 AT1G11590.1 39.910 446 239 7 106 541 98 524 5.20e-102 317
Msa1353940 AT4G15980.1 46.746 338 176 4 203 539 366 700 2.13e-100 318
Msa1353940 AT4G15980.2 46.746 338 176 4 203 539 376 710 2.71e-100 318
Msa1353940 AT5G20860.1 36.240 516 251 12 28 520 29 489 1.04e-99 311
Msa1353940 AT5G09760.1 35.373 523 300 12 33 534 46 551 4.64e-93 295
Msa1353940 AT5G09760.2 35.373 523 300 12 33 534 72 577 1.28e-92 295
Msa1353940 AT1G11370.1 49.225 258 130 1 216 473 32 288 4.61e-87 271
Msa1353940 AT2G47030.1 43.132 364 182 7 196 541 232 588 1.23e-85 276
Msa1353940 AT3G62170.1 36.709 474 259 11 100 541 124 588 7.09e-85 275
Msa1353940 AT2G47040.1 38.767 454 250 12 103 541 155 595 1.04e-84 275
Msa1353940 AT5G64640.1 36.865 453 244 12 104 531 164 599 1.42e-84 274
Msa1353940 AT5G19730.1 37.676 284 151 6 258 530 113 381 1.30e-50 178
Msa1353940 AT3G29090.1 35.439 285 160 6 230 503 7 278 2.34e-48 170
Msa1353940 AT1G05310.1 34.713 314 176 7 230 530 91 388 1.16e-46 168
Msa1353940 AT2G36710.1 33.117 308 186 5 230 530 91 385 5.90e-44 161
Msa1353940 AT2G36700.1 31.579 304 189 5 232 530 44 333 7.64e-44 159
Msa1353940 AT1G44980.1 42.512 207 117 2 188 394 37 241 2.80e-42 152
Msa1353940 AT2G21610.1 31.126 302 187 6 230 530 51 332 1.48e-40 150
Msa1353940 AT2G21610.2 31.126 302 187 6 230 530 56 337 2.28e-40 150
Msa1353940 AT3G17060.1 28.869 336 205 8 210 530 20 336 1.84e-39 147
Msa1353940 AT3G24130.1 31.333 300 179 7 215 503 21 304 1.34e-38 144
Msa1353940 AT5G18990.1 32.667 300 170 7 215 503 21 299 6.73e-38 142
Msa1353940 AT5G47500.1 30.530 321 195 7 223 531 54 358 1.64e-37 142
Msa1353940 AT5G07430.1 30.323 310 196 6 225 530 62 355 4.15e-37 141
Msa1353940 AT5G55590.1 34.069 317 179 9 227 530 79 378 1.18e-35 137
Msa1353940 AT5G26810.1 30.794 315 191 9 230 531 34 334 2.26e-34 133
Msa1353940 AT5G55590.2 34.231 260 146 7 227 474 79 325 7.85e-34 131
Msa1353940 AT3G49220.2 32.680 306 173 14 35 325 135 422 2.59e-33 132
Msa1353940 AT2G19150.1 28.395 324 209 8 221 537 32 339 1.21e-32 128
Msa1353940 AT5G61680.1 28.710 310 199 8 226 530 66 358 1.67e-32 128
Msa1353940 AT5G07420.1 28.308 325 207 9 213 530 50 355 1.82e-31 125
Msa1353940 AT2G47280.1 29.487 312 192 8 230 531 35 328 2.38e-30 121
Msa1353940 AT2G19150.2 29.344 259 167 6 221 474 32 279 2.36e-28 114
Msa1353940 AT1G69940.1 26.045 311 207 8 225 530 63 355 3.45e-27 113
Msa1353940 AT5G07410.1 26.367 311 206 8 225 530 63 355 5.85e-27 112

Find 150 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCCATACTGGCTTTC+AGG 0.135570 tig0014103:-159133 Msa1353940:CDS
GCTGGTGATTCTTGGATTTC+AGG 0.138272 tig0014103:-157165 Msa1353940:CDS
GTGAAGAATTTAATGATATT+TGG 0.168927 tig0014103:-158908 Msa1353940:CDS
TTCTTCTTCTTTCCATGATA+TGG 0.204942 tig0014103:-159557 Msa1353940:CDS
AATAATTATTGCAGCTGTTA+TGG 0.230375 tig0014103:-157817 Msa1353940:intron
GCCAAGTTAATTTATCATTT+AGG 0.239565 tig0014103:+159371 None:intergenic
TGTGAATATTGATTAATTTC+TGG 0.257072 tig0014103:-157116 Msa1353940:exon
GCATTGAGTACACTTTATTA+TGG 0.267204 tig0014103:-157303 Msa1353940:CDS
ACAATCTTCCCATGCTGATT+TGG 0.282004 tig0014103:+159450 None:intergenic
TTTCCATGATATGGCTCTTA+AGG 0.287787 tig0014103:-159548 Msa1353940:CDS
GCCTGAAGCAGCTTTCTATC+TGG 0.308018 tig0014103:+159109 None:intergenic
AATCATTTGCTGCGGTTATA+CGG 0.315468 tig0014103:+157460 None:intergenic
AATATGAGTTTATTCATATT+AGG 0.319561 tig0014103:+159721 None:intergenic
CTGGTGATTCTTGGATTTCA+GGG 0.333928 tig0014103:-157164 Msa1353940:CDS
ATAATTATTGCAGCTGTTAT+GGG 0.345443 tig0014103:-157816 Msa1353940:intron
TCATCAAGTACTAAACAAAA+TGG 0.356786 tig0014103:-159178 Msa1353940:CDS
ATGGACTCCACTATTGTTAC+TGG 0.363587 tig0014103:-158878 Msa1353940:CDS
GGAACTATTGATTTCATCTT+CGG 0.369134 tig0014103:-157612 Msa1353940:CDS
CATGGAAAGAAGAAGAATCT+AGG 0.371037 tig0014103:+159563 None:intergenic
TATCAGATAAATTTCCATAC+TGG 0.372948 tig0014103:-159141 Msa1353940:CDS
TTTGATAAATTCACAAGGTT+GGG 0.373566 tig0014103:-157346 Msa1353940:CDS
TCTGGCCCTGAAAGCCAGTA+TGG 0.384412 tig0014103:+159127 None:intergenic
AATTTCCATACTGGCTTTCA+GGG 0.391328 tig0014103:-159132 Msa1353940:CDS
GCCAGATAGAAAGCTGCTTC+AGG 0.395026 tig0014103:-159110 Msa1353940:CDS
AATTCAGTTAAAACATATCT+TGG 0.398697 tig0014103:-157423 Msa1353940:CDS
CTTCAGGCAACACCAGGGTC+AGG 0.401448 tig0014103:-159094 Msa1353940:CDS
GGTAATCATAATGCTATAGA+TGG 0.406520 tig0014103:-158857 Msa1353940:CDS
TGTTGCAGTCGCGGTAGAAT+TGG 0.411073 tig0014103:+157640 None:intergenic
GTAATGAAAAGTAATCTTGA+TGG 0.411444 tig0014103:-157369 Msa1353940:CDS
GTTGTCATTCATGTGAAATC+AGG 0.412213 tig0014103:-158965 Msa1353940:CDS
TGTTCTTCCTTGTGCTGTTA+CGG 0.414972 tig0014103:+157521 None:intergenic
ATACTGGTGGAAGAGTTAAT+TGG 0.417090 tig0014103:-157251 Msa1353940:CDS
GTTTGATAAATTCACAAGGT+TGG 0.422089 tig0014103:-157347 Msa1353940:CDS
TATTGTCATGTAGTATTTGT+TGG 0.422147 tig0014103:-156950 Msa1353940:three_prime_UTR
TTTGGAAGAGCTAGGTATAT+AGG 0.422385 tig0014103:+159794 None:intergenic
AGACAGTGTGTTGGTGGTGG+TGG 0.423692 tig0014103:+159588 None:intergenic
GCGGTTGGCGTAAGCATATA+AGG 0.426322 tig0014103:+157662 None:intergenic
CGCAGCAAATGATTTGAAAC+CGG 0.431620 tig0014103:-157451 Msa1353940:CDS
TACAAGTTTGTGTGCTTCAA+TGG 0.434838 tig0014103:+159510 None:intergenic
GTTGGTGGTGGTGCTAATAC+TGG 0.437055 tig0014103:-157267 Msa1353940:CDS
CTTGGTGCTGGCCCTGACCC+TGG 0.440453 tig0014103:+159082 None:intergenic
CAAGCAGTAGCTCTCCGTTC+CGG 0.442034 tig0014103:-157735 Msa1353940:CDS
GGGAAGTAGTTTAAGTGAGA+TGG 0.442427 tig0014103:+159289 None:intergenic
GGTGTCTTGGTAGCCTTTGA+AGG 0.444283 tig0014103:+157683 None:intergenic
TTTGAAATTATTTAATTCCA+TGG 0.450557 tig0014103:+159483 None:intergenic
GTGTTCTGTTGGTTGCTCAT+TGG 0.453378 tig0014103:+157543 None:intergenic
ACAAGGTTGGGCTCCATGGA+TGG 0.456078 tig0014103:-157334 Msa1353940:CDS
TGGTTACACACATGAGGGTA+TGG 0.456641 tig0014103:+159622 None:intergenic
GTGAAGTTGCTTAACATGGA+TGG 0.463236 tig0014103:+159268 None:intergenic
CAAGGTTGGGCTCCATGGAT+GGG 0.470284 tig0014103:-157333 Msa1353940:CDS
ATTCACAAGGTTGGGCTCCA+TGG 0.471108 tig0014103:-157338 Msa1353940:CDS
ATTGCAGCTGTTATGGGAGA+TGG 0.472604 tig0014103:-157810 Msa1353940:intron
ATTGTCATGTAGTATTTGTT+GGG 0.472757 tig0014103:-156949 Msa1353940:three_prime_UTR
ACAAGTTTGATGATTGGTAA+TGG 0.473868 tig0014103:+159339 None:intergenic
GGACATGACCTTTGAGAATA+CGG 0.477411 tig0014103:-157775 Msa1353940:CDS
AATGATATTTGGAGATGGAA+TGG 0.477734 tig0014103:-158897 Msa1353940:CDS
GTTGCAGCTGCTAAGCTTAG+TGG 0.477922 tig0014103:-158998 Msa1353940:CDS
GTTTGTGTGCTTCAATGGCT+TGG 0.481327 tig0014103:+159515 None:intergenic
AAGCTGCTTCAGGCAACACC+AGG 0.481870 tig0014103:-159100 Msa1353940:CDS
TTCTACCGTTGTGCCTTCAA+AGG 0.487774 tig0014103:-157696 Msa1353940:CDS
TGCTTCAATGGCTTGGTCCA+TGG 0.489763 tig0014103:+159522 None:intergenic
ACTTGAAATGATGATGACTT+TGG 0.499085 tig0014103:-157036 Msa1353940:three_prime_UTR
AGATTGTTTAGAGCTTTATG+AGG 0.504717 tig0014103:-159434 Msa1353940:CDS
AGTGGTGTGCCGTTTGATGC+AGG 0.508356 tig0014103:-157141 Msa1353940:CDS
ACCTAAATGATAAATTAACT+TGG 0.508748 tig0014103:-159372 Msa1353940:CDS
ACAATATCAGCTCTTGGTGC+TGG 0.510769 tig0014103:+159070 None:intergenic
TGCAGTCGCGGTAGAATTGG+CGG 0.511251 tig0014103:+157643 None:intergenic
TGAAGTTGCTTAACATGGAT+GGG 0.511299 tig0014103:+159269 None:intergenic
TTGCAGCTGCTAAGCTTAGT+GGG 0.511688 tig0014103:-158997 Msa1353940:CDS
GGTAGACAGTGTGTTGGTGG+TGG 0.512332 tig0014103:+159585 None:intergenic
GATATTGTTGTTGCACAAGA+TGG 0.515434 tig0014103:-159055 Msa1353940:CDS
TGGTGGTGGTTCCAATGTAA+TGG 0.517543 tig0014103:+159602 None:intergenic
GTTCTTGAATATTTCTGCCA+TGG 0.518439 tig0014103:+157396 None:intergenic
GGTGGAAGAGTTAATTGGCC+TGG 0.520232 tig0014103:-157246 Msa1353940:CDS
ATGATTACCAGTAACAATAG+TGG 0.522826 tig0014103:+158871 None:intergenic
ATGGAAAGAAGAAGAATCTA+GGG 0.522868 tig0014103:+159564 None:intergenic
GGGAGATGGTTTCATAGCTA+AGG 0.523196 tig0014103:-157796 Msa1353940:CDS
ATTCACAAACCTGCATCAAA+CGG 0.526344 tig0014103:+157132 None:intergenic
TTTAGTGAAGTTGCTTAACA+TGG 0.527598 tig0014103:+159264 None:intergenic
TACCGCGACTGCAACATCTA+CGG 0.528232 tig0014103:-157633 Msa1353940:CDS
TGACCTTAAGAGCCATATCA+TGG 0.529885 tig0014103:+159545 None:intergenic
CGTAAGCATATAAGGTGTCT+TGG 0.533953 tig0014103:+157670 None:intergenic
GGTTGGGCTCCATGGATGGG+TGG 0.536463 tig0014103:-157330 Msa1353940:CDS
TATAGTGAAAGAAATAAAAG+TGG 0.536866 tig0014103:-157078 Msa1353940:three_prime_UTR
TGTTATTACTAATCCTTCTG+AGG 0.537907 tig0014103:-157217 Msa1353940:CDS
GTGCTGTTACGGTGTTCTGT+TGG 0.539536 tig0014103:+157532 None:intergenic
TGAAGTTAGAGTGTTGCTAA+TGG 0.541067 tig0014103:+159222 None:intergenic
TATGGTGAATATATGAATGT+TGG 0.547151 tig0014103:-157285 Msa1353940:CDS
TGGGTGTTTGCCTTGTACAA+AGG 0.550451 tig0014103:-156930 Msa1353940:three_prime_UTR
TCTTGTGTAACCTTTGTACA+AGG 0.550590 tig0014103:+156920 None:intergenic
GGTTACACACATGAGGGTAT+GGG 0.553090 tig0014103:+159623 None:intergenic
GAAAATTTAACAGCCTCAGA+AGG 0.553132 tig0014103:+157204 None:intergenic
AATTTAATGATATTTGGAGA+TGG 0.553852 tig0014103:-158902 Msa1353940:CDS
CAGAACACCGTAACAGCACA+AGG 0.556039 tig0014103:-157528 Msa1353940:CDS
GCTGCTAAGCTTAGTGGGAA+AGG 0.557812 tig0014103:-158992 Msa1353940:CDS
GATTCTTGGATTTCAGGGAG+TGG 0.559486 tig0014103:-157159 Msa1353940:CDS
GCAACAACAATATCAGCTCT+TGG 0.560130 tig0014103:+159064 None:intergenic
AGTACTTGATGATGTGGATG+AGG 0.565120 tig0014103:+159189 None:intergenic
TTGTTTAGTACTTGATGATG+TGG 0.565122 tig0014103:+159183 None:intergenic
CAGAGTGATCGGCGCCGGAA+CGG 0.565710 tig0014103:+157721 None:intergenic
GTTGTTGCACAAGATGGAAG+TGG 0.568022 tig0014103:-159049 Msa1353940:CDS
AAAAGTGGTTGATGATTCAG+TGG 0.568443 tig0014103:-157063 Msa1353940:three_prime_UTR
GAAAACAGAGTGATCGGCGC+CGG 0.568686 tig0014103:+157716 None:intergenic
CATGTGTGTAACCATTACAT+TGG 0.569476 tig0014103:-159613 Msa1353940:CDS
GGGTCCTGCCGTATTCTCAA+AGG 0.569689 tig0014103:+157767 None:intergenic
GTCGCGGTAGAATTGGCGGT+TGG 0.570719 tig0014103:+157647 None:intergenic
AGTAGTTTAAGTGAGATGGA+AGG 0.574197 tig0014103:+159293 None:intergenic
GTTACACACATGAGGGTATG+GGG 0.574452 tig0014103:+159624 None:intergenic
TAGGGTAGACAGTGTGTTGG+TGG 0.577198 tig0014103:+159582 None:intergenic
CGGAACGGAGAGCTACTGCT+TGG 0.579824 tig0014103:+157736 None:intergenic
TAAATATATTTGGAAGAGCT+AGG 0.580761 tig0014103:+159786 None:intergenic
AATGCAAAACCACCCATCCA+TGG 0.580980 tig0014103:+157321 None:intergenic
AAGAGCTAGGTATATAGGAA+TGG 0.581001 tig0014103:+159799 None:intergenic
TGCAACAACACCCTCTGAGA+TGG 0.581283 tig0014103:+159015 None:intergenic
TTCCGTAGATGTTGCAGTCG+CGG 0.586032 tig0014103:+157631 None:intergenic
TCAAGTACTAAACAAAATGG+AGG 0.588650 tig0014103:-159175 Msa1353940:CDS
AAAAGAGGATGGTGAAAGCG+TGG 0.591353 tig0014103:-156998 Msa1353940:three_prime_UTR
GGTGAATATATGAATGTTGG+TGG 0.591996 tig0014103:-157282 Msa1353940:CDS
TGGTGGTGACAATTGAAGAT+GGG 0.594007 tig0014103:-156978 Msa1353940:three_prime_UTR
ACAAGCGTGCCAAATCAGCA+TGG 0.597886 tig0014103:-159459 Msa1353940:CDS
AGGATATGCAATATTGTGCA+TGG 0.599200 tig0014103:+159741 None:intergenic
AACTATAAGACCATCTCAGA+GGG 0.599247 tig0014103:-159025 Msa1353940:CDS
ATGACCTTTGAGAATACGGC+AGG 0.599978 tig0014103:-157771 Msa1353940:CDS
GTGGTGGTGACAATTGAAGA+TGG 0.602928 tig0014103:-156979 Msa1353940:three_prime_UTR
TTCAGGCAACACCAGGGTCA+GGG 0.603563 tig0014103:-159093 Msa1353940:CDS
CAAGCGTGCCAAATCAGCAT+GGG 0.604901 tig0014103:-159458 Msa1353940:CDS
ATGTAATGGTTACACACATG+AGG 0.605826 tig0014103:+159616 None:intergenic
ACGGTAGAAAACAGAGTGAT+CGG 0.606991 tig0014103:+157710 None:intergenic
GATGATGTGGATGAGGATGA+TGG 0.609727 tig0014103:+159196 None:intergenic
ATCTAGGGTAGACAGTGTGT+TGG 0.618112 tig0014103:+159579 None:intergenic
CTAAACAAAATGGAGGGAGA+AGG 0.620123 tig0014103:-159168 Msa1353940:CDS
GAATATATGAATGTTGGTGG+TGG 0.621736 tig0014103:-157279 Msa1353940:CDS
CGGTTTCAAATCATTTGCTG+CGG 0.623680 tig0014103:+157452 None:intergenic
CAAGTACTAAACAAAATGGA+GGG 0.628274 tig0014103:-159174 Msa1353940:CDS
GGTATATAGGAATGGATGAG+TGG 0.630123 tig0014103:+159807 None:intergenic
TGTAATGGTTACACACATGA+GGG 0.637885 tig0014103:+159617 None:intergenic
ACAGATCCAAATGAAAACAC+AGG 0.641536 tig0014103:-157501 Msa1353940:CDS
AAACATATCTTGGTAGACCA+TGG 0.646826 tig0014103:-157413 Msa1353940:CDS
AAAGTGGTTGATGATTCAGT+GGG 0.655206 tig0014103:-157062 Msa1353940:three_prime_UTR
GGTGGTGGTGCTAATACTGG+TGG 0.661832 tig0014103:-157264 Msa1353940:CDS
ATGGCAGAAATATTCAAGAA+CGG 0.671890 tig0014103:-157394 Msa1353940:CDS
TTAGTAATAACATGAAAACC+AGG 0.674041 tig0014103:+157228 None:intergenic
ACACAAACTTGTATCAACCA+TGG 0.676347 tig0014103:-159500 Msa1353940:CDS
GGTGGTGACAATTGAAGATG+GGG 0.677712 tig0014103:-156977 Msa1353940:three_prime_UTR
TATGGCTCTTAAGGTCACCA+TGG 0.681394 tig0014103:-159539 Msa1353940:CDS
GGTAGCCTTTGAAGGCACAA+CGG 0.688392 tig0014103:+157691 None:intergenic
AGCTGCTTCAGGCAACACCA+GGG 0.695457 tig0014103:-159099 Msa1353940:CDS
GATGGTTTGATAAATTCACA+AGG 0.696704 tig0014103:-157351 Msa1353940:CDS
AAACTATAAGACCATCTCAG+AGG 0.701800 tig0014103:-159026 Msa1353940:CDS
AGAGGATGGTGAAAGCGTGG+TGG 0.727927 tig0014103:-156995 Msa1353940:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATTTTTTTTTTTTTTTT+GGG - tig0014103:159752-159771 Msa1353940:exon 0.0%
!!! TTTTAATGGCTAAATATATT+TGG + tig0014103:156852-156871 None:intergenic 15.0%
!! AATATGAGTTTATTCATATT+AGG + tig0014103:156907-156926 None:intergenic 15.0%
!!! TTTGAAATTATTTAATTCCA+TGG + tig0014103:157145-157164 None:intergenic 15.0%
!! AACAAATGTTAAATAAAGTA+GGG + tig0014103:158219-158238 None:intergenic 15.0%
!! AAACAAATGTTAAATAAAGT+AGG + tig0014103:158220-158239 None:intergenic 15.0%
!! TTTAGTGAATATTGTTAAAA+AGG + tig0014103:158550-158569 None:intergenic 15.0%
!!! TTGTTTTGTATAAAAAAAAG+AGG - tig0014103:159612-159631 Msa1353940:CDS 15.0%
!!! GGTATCGAATTTTTTTTTAA+TGG + tig0014103:156866-156885 None:intergenic 20.0%
!! ACCTAAATGATAAATTAACT+TGG - tig0014103:157253-157272 Msa1353940:CDS 20.0%
!!! ATTTTTACAAGTTTGATGAT+TGG + tig0014103:157295-157314 None:intergenic 20.0%
!! AATCATCAAACTTGTAAAAA+TGG - tig0014103:157294-157313 Msa1353940:CDS 20.0%
!! GTGAAGAATTTAATGATATT+TGG - tig0014103:157717-157736 Msa1353940:CDS 20.0%
!! AATTTAATGATATTTGGAGA+TGG - tig0014103:157723-157742 Msa1353940:CDS 20.0%
!! TATGAATAGATTAGTGATTA+AGG + tig0014103:157837-157856 None:intergenic 20.0%
!!! AACTTTTTACATTAATTGAC+CGG + tig0014103:158597-158616 None:intergenic 20.0%
!! GGTCATAATTACTTTATTAA+AGG + tig0014103:158683-158702 None:intergenic 20.0%
!! AATTCAGTTAAAACATATCT+TGG - tig0014103:159202-159221 Msa1353940:CDS 20.0%
!!! ATTTTCTGTTGGTAATTTTT+TGG - tig0014103:159438-159457 Msa1353940:CDS 20.0%
!!! TGTGAATATTGATTAATTTC+TGG - tig0014103:159509-159528 Msa1353940:CDS 20.0%
!! TATAGTGAAAGAAATAAAAG+TGG - tig0014103:159547-159566 Msa1353940:CDS 20.0%
!! TTTGTATAAAAAAAAGAGGA+TGG - tig0014103:159616-159635 Msa1353940:CDS 20.0%
!!! TTTTTTTTTTTTTTGGGTCT+AGG - tig0014103:159758-159777 Msa1353940:exon 20.0%
! TAAATATATTTGGAAGAGCT+AGG + tig0014103:156842-156861 None:intergenic 25.0%
! GCCAAGTTAATTTATCATTT+AGG + tig0014103:157257-157276 None:intergenic 25.0%
! TCATCAAGTACTAAACAAAA+TGG - tig0014103:157447-157466 Msa1353940:CDS 25.0%
! TATCAGATAAATTTCCATAC+TGG - tig0014103:157484-157503 Msa1353940:CDS 25.0%
! CACTAGAAAATATATCACTT+TGG - tig0014103:157999-158018 Msa1353940:intron 25.0%
! TTTATGTACAACTTGTGTTA+AGG - tig0014103:158052-158071 Msa1353940:intron 25.0%
!! GATGTAAAAGGTATAATTGT+TGG + tig0014103:158407-158426 None:intergenic 25.0%
! AACAATATTCACTAAAGTTC+TGG - tig0014103:158554-158573 Msa1353940:intron 25.0%
!!! GTAAAGAATTTTTTGCACTA+TGG + tig0014103:158741-158760 None:intergenic 25.0%
! AATAATTATTGCAGCTGTTA+TGG - tig0014103:158808-158827 Msa1353940:intron 25.0%
! ATAATTATTGCAGCTGTTAT+GGG - tig0014103:158809-158828 Msa1353940:intron 25.0%
! GTAATGAAAAGTAATCTTGA+TGG - tig0014103:159256-159275 Msa1353940:CDS 25.0%
! TTTGATAAATTCACAAGGTT+GGG - tig0014103:159279-159298 Msa1353940:CDS 25.0%
! TATGGTGAATATATGAATGT+TGG - tig0014103:159340-159359 Msa1353940:CDS 25.0%
! TTAGTAATAACATGAAAACC+AGG + tig0014103:159400-159419 None:intergenic 25.0%
! TATTGTCATGTAGTATTTGT+TGG - tig0014103:159675-159694 Msa1353940:CDS 25.0%
! ATTGTCATGTAGTATTTGTT+GGG - tig0014103:159676-159695 Msa1353940:CDS 25.0%
ATGGAAAGAAGAAGAATCTA+GGG + tig0014103:157064-157083 None:intergenic 30.0%
TTCTTCTTCTTTCCATGATA+TGG - tig0014103:157068-157087 Msa1353940:three_prime_UTR 30.0%
! AGATTGTTTAGAGCTTTATG+AGG - tig0014103:157191-157210 Msa1353940:CDS 30.0%
! ACAAGTTTGATGATTGGTAA+TGG + tig0014103:157289-157308 None:intergenic 30.0%
TTTAGTGAAGTTGCTTAACA+TGG + tig0014103:157364-157383 None:intergenic 30.0%
!! TTGTTTAGTACTTGATGATG+TGG + tig0014103:157445-157464 None:intergenic 30.0%
TCAAGTACTAAACAAAATGG+AGG - tig0014103:157450-157469 Msa1353940:CDS 30.0%
CAAGTACTAAACAAAATGGA+GGG - tig0014103:157451-157470 Msa1353940:CDS 30.0%
AATGATATTTGGAGATGGAA+TGG - tig0014103:157728-157747 Msa1353940:CDS 30.0%
ATGATTACCAGTAACAATAG+TGG + tig0014103:157757-157776 None:intergenic 30.0%
GGTAATCATAATGCTATAGA+TGG - tig0014103:157768-157787 Msa1353940:CDS 30.0%
! AGTTCGTTTTCTATATGCAT+GGG - tig0014103:157906-157925 Msa1353940:intron 30.0%
TTGAGTAACTACTTGTCATA+TGG - tig0014103:158095-158114 Msa1353940:intron 30.0%
! CTTTTCTACTTTCTCTCAAA+AGG - tig0014103:158247-158266 Msa1353940:intron 30.0%
!!! TTTTCTGAGACTGATGTAAA+AGG + tig0014103:158419-158438 None:intergenic 30.0%
CCTGAACATTTAATAGTATG+TGG + tig0014103:158704-158723 None:intergenic 30.0%
CCACATACTATTAAATGTTC+AGG - tig0014103:158701-158720 Msa1353940:intron 30.0%
!! GGAACTATTGATTTCATCTT+CGG - tig0014103:159013-159032 Msa1353940:CDS 30.0%
! ACAATTCCTGTGTTTTCATT+TGG + tig0014103:159133-159152 None:intergenic 30.0%
!!! GTTTTAACTGAATTCTGAAC+CGG + tig0014103:159196-159215 None:intergenic 30.0%
ATGGCAGAAATATTCAAGAA+CGG - tig0014103:159231-159250 Msa1353940:CDS 30.0%
GATGGTTTGATAAATTCACA+AGG - tig0014103:159274-159293 Msa1353940:CDS 30.0%
GTTTGATAAATTCACAAGGT+TGG - tig0014103:159278-159297 Msa1353940:CDS 30.0%
GCATTGAGTACACTTTATTA+TGG - tig0014103:159322-159341 Msa1353940:CDS 30.0%
TGTTATTACTAATCCTTCTG+AGG - tig0014103:159408-159427 Msa1353940:CDS 30.0%
! ACTTGAAATGATGATGACTT+TGG - tig0014103:159589-159608 Msa1353940:CDS 30.0%
AAGAGCTAGGTATATAGGAA+TGG + tig0014103:156829-156848 None:intergenic 35.0%
TTTGGAAGAGCTAGGTATAT+AGG + tig0014103:156834-156853 None:intergenic 35.0%
AGGATATGCAATATTGTGCA+TGG + tig0014103:156887-156906 None:intergenic 35.0%
GCACAAACAAGAGAGAAAAA+AGG + tig0014103:156954-156973 None:intergenic 35.0%
!! TTTTCTCTCTTGTTTGTGCA+TGG - tig0014103:156955-156974 Msa1353940:three_prime_UTR 35.0%
TGTAATGGTTACACACATGA+GGG + tig0014103:157011-157030 None:intergenic 35.0%
ATGTAATGGTTACACACATG+AGG + tig0014103:157012-157031 None:intergenic 35.0%
CATGTGTGTAACCATTACAT+TGG - tig0014103:157012-157031 Msa1353940:three_prime_UTR 35.0%
CATGGAAAGAAGAAGAATCT+AGG + tig0014103:157065-157084 None:intergenic 35.0%
TTTCCATGATATGGCTCTTA+AGG - tig0014103:157077-157096 Msa1353940:three_prime_UTR 35.0%
TACAAGTTTGTGTGCTTCAA+TGG + tig0014103:157118-157137 None:intergenic 35.0%
ACACAAACTTGTATCAACCA+TGG - tig0014103:157125-157144 Msa1353940:exon 35.0%
AGTAGTTTAAGTGAGATGGA+AGG + tig0014103:157335-157354 None:intergenic 35.0%
TGAAGTTGCTTAACATGGAT+GGG + tig0014103:157359-157378 None:intergenic 35.0%
!! TGAAGTTAGAGTGTTGCTAA+TGG + tig0014103:157406-157425 None:intergenic 35.0%
!! AAATTTCCATACTGGCTTTC+AGG - tig0014103:157492-157511 Msa1353940:CDS 35.0%
!! AATTTCCATACTGGCTTTCA+GGG - tig0014103:157493-157512 Msa1353940:CDS 35.0%
! GATATTGTTGTTGCACAAGA+TGG - tig0014103:157570-157589 Msa1353940:CDS 35.0%
AAACTATAAGACCATCTCAG+AGG - tig0014103:157599-157618 Msa1353940:CDS 35.0%
AACTATAAGACCATCTCAGA+GGG - tig0014103:157600-157619 Msa1353940:CDS 35.0%
GTTGTCATTCATGTGAAATC+AGG - tig0014103:157660-157679 Msa1353940:CDS 35.0%
!! ACTTTTCGTTCAGCTACTTT+TGG - tig0014103:157798-157817 Msa1353940:CDS 35.0%
! CAGTTCGTTTTCTATATGCA+TGG - tig0014103:157905-157924 Msa1353940:intron 35.0%
GGGAGAAATCATATGATCAA+TGG - tig0014103:157926-157945 Msa1353940:intron 35.0%
TGCATGTCTCTATCTTAATG+GGG + tig0014103:158025-158044 None:intergenic 35.0%
CTGCATGTCTCTATCTTAAT+GGG + tig0014103:158026-158045 None:intergenic 35.0%
ACTGCATGTCTCTATCTTAA+TGG + tig0014103:158027-158046 None:intergenic 35.0%
AGGTATAATTGTTGGTCGTA+TGG + tig0014103:158399-158418 None:intergenic 35.0%
! TACATTAATTGACCGGTTCA+TGG + tig0014103:158590-158609 None:intergenic 35.0%
! CGAAGATGTTGCAATTTTGA+AGG + tig0014103:159054-159073 None:intergenic 35.0%
ACAGATCCAAATGAAAACAC+AGG - tig0014103:159124-159143 Msa1353940:CDS 35.0%
! AATCATTTGCTGCGGTTATA+CGG + tig0014103:159168-159187 None:intergenic 35.0%
AAACATATCTTGGTAGACCA+TGG - tig0014103:159212-159231 Msa1353940:CDS 35.0%
! GTTCTTGAATATTTCTGCCA+TGG + tig0014103:159232-159251 None:intergenic 35.0%
GGTGAATATATGAATGTTGG+TGG - tig0014103:159343-159362 Msa1353940:CDS 35.0%
! GAATATATGAATGTTGGTGG+TGG - tig0014103:159346-159365 Msa1353940:CDS 35.0%
ATACTGGTGGAAGAGTTAAT+TGG - tig0014103:159374-159393 Msa1353940:CDS 35.0%
GAAAATTTAACAGCCTCAGA+AGG + tig0014103:159424-159443 None:intergenic 35.0%
! GAGGCTGTTAAATTTTCTGT+TGG - tig0014103:159427-159446 Msa1353940:CDS 35.0%
!!! TCTGTTGGTAATTTTTTGGC+TGG - tig0014103:159442-159461 Msa1353940:CDS 35.0%
!!! ATTTTTTGGCTGGTGATTCT+TGG - tig0014103:159452-159471 Msa1353940:CDS 35.0%
ATTCACAAACCTGCATCAAA+CGG + tig0014103:159496-159515 None:intergenic 35.0%
!! AAAAGTGGTTGATGATTCAG+TGG - tig0014103:159562-159581 Msa1353940:CDS 35.0%
!! AAAGTGGTTGATGATTCAGT+GGG - tig0014103:159563-159582 Msa1353940:CDS 35.0%
TCTTGTGTAACCTTTGTACA+AGG + tig0014103:159708-159727 None:intergenic 35.0%
GGTATATAGGAATGGATGAG+TGG + tig0014103:156821-156840 None:intergenic 40.0%
TGACCTTAAGAGCCATATCA+TGG + tig0014103:157083-157102 None:intergenic 40.0%
! ACAATCTTCCCATGCTGATT+TGG + tig0014103:157178-157197 None:intergenic 40.0%
GGGAAGTAGTTTAAGTGAGA+TGG + tig0014103:157339-157358 None:intergenic 40.0%
GTGAAGTTGCTTAACATGGA+TGG + tig0014103:157360-157379 None:intergenic 40.0%
!! AGTACTTGATGATGTGGATG+AGG + tig0014103:157439-157458 None:intergenic 40.0%
CTAAACAAAATGGAGGGAGA+AGG - tig0014103:157457-157476 Msa1353940:CDS 40.0%
GCAACAACAATATCAGCTCT+TGG + tig0014103:157564-157583 None:intergenic 40.0%
ATGGACTCCACTATTGTTAC+TGG - tig0014103:157747-157766 Msa1353940:CDS 40.0%
GGAGTTTAATCTCCATGAAC+CGG - tig0014103:158575-158594 Msa1353940:intron 40.0%
! GGACATGACCTTTGAGAATA+CGG - tig0014103:158850-158869 Msa1353940:CDS 40.0%
!!! AGCTACTGCTTGGTGTTTTT+GGG + tig0014103:158882-158901 None:intergenic 40.0%
ACGGTAGAAAACAGAGTGAT+CGG + tig0014103:158918-158937 None:intergenic 40.0%
! CGTAAGCATATAAGGTGTCT+TGG + tig0014103:158958-158977 None:intergenic 40.0%
! TGTTCTTCCTTGTGCTGTTA+CGG + tig0014103:159107-159126 None:intergenic 40.0%
! CGGTTTCAAATCATTTGCTG+CGG + tig0014103:159176-159195 None:intergenic 40.0%
! CGCAGCAAATGATTTGAAAC+CGG - tig0014103:159174-159193 Msa1353940:CDS 40.0%
CTGGTGATTCTTGGATTTCA+GGG - tig0014103:159461-159480 Msa1353940:CDS 40.0%
! TGGTGGTGACAATTGAAGAT+GGG - tig0014103:159647-159666 Msa1353940:CDS 40.0%
! GTTACACACATGAGGGTATG+GGG + tig0014103:157004-157023 None:intergenic 45.0%
! GGTTACACACATGAGGGTAT+GGG + tig0014103:157005-157024 None:intergenic 45.0%
TGGTTACACACATGAGGGTA+TGG + tig0014103:157006-157025 None:intergenic 45.0%
!! TGGTGGTGGTTCCAATGTAA+TGG + tig0014103:157026-157045 None:intergenic 45.0%
! ATCTAGGGTAGACAGTGTGT+TGG + tig0014103:157049-157068 None:intergenic 45.0%
TATGGCTCTTAAGGTCACCA+TGG - tig0014103:157086-157105 Msa1353940:three_prime_UTR 45.0%
GTTTGTGTGCTTCAATGGCT+TGG + tig0014103:157113-157132 None:intergenic 45.0%
GATGATGTGGATGAGGATGA+TGG + tig0014103:157432-157451 None:intergenic 45.0%
! ACAATATCAGCTCTTGGTGC+TGG + tig0014103:157558-157577 None:intergenic 45.0%
GTTGTTGCACAAGATGGAAG+TGG - tig0014103:157576-157595 Msa1353940:CDS 45.0%
TTGCAGCTGCTAAGCTTAGT+GGG - tig0014103:157628-157647 Msa1353940:CDS 45.0%
ATTGCAGCTGTTATGGGAGA+TGG - tig0014103:158815-158834 Msa1353940:intron 45.0%
GGGAGATGGTTTCATAGCTA+AGG - tig0014103:158829-158848 Msa1353940:CDS 45.0%
! ATGACCTTTGAGAATACGGC+AGG - tig0014103:158854-158873 Msa1353940:CDS 45.0%
!!! GCTACTGCTTGGTGTTTTTG+GGG + tig0014103:158881-158900 None:intergenic 45.0%
!!! GAGCTACTGCTTGGTGTTTT+TGG + tig0014103:158883-158902 None:intergenic 45.0%
TTCTACCGTTGTGCCTTCAA+AGG - tig0014103:158929-158948 Msa1353940:CDS 45.0%
! GTGTTCTGTTGGTTGCTCAT+TGG + tig0014103:159085-159104 None:intergenic 45.0%
AATGCAAAACCACCCATCCA+TGG + tig0014103:159307-159326 None:intergenic 45.0%
GCTGGTGATTCTTGGATTTC+AGG - tig0014103:159460-159479 Msa1353940:CDS 45.0%
GATTCTTGGATTTCAGGGAG+TGG - tig0014103:159466-159485 Msa1353940:CDS 45.0%
AAAAGAGGATGGTGAAAGCG+TGG - tig0014103:159627-159646 Msa1353940:CDS 45.0%
! GTGGTGGTGACAATTGAAGA+TGG - tig0014103:159646-159665 Msa1353940:CDS 45.0%
GGTGGTGACAATTGAAGATG+GGG - tig0014103:159648-159667 Msa1353940:CDS 45.0%
TGGGTGTTTGCCTTGTACAA+AGG - tig0014103:159695-159714 Msa1353940:CDS 45.0%
!! CAAAAAAAAAAAAAAAATAA+TGG + tig0014103:159753-159772 None:intergenic 5.0%
!!! CATTATTTTTTTTTTTTTTT+TGG - tig0014103:159751-159770 Msa1353940:exon 5.0%
TAGGGTAGACAGTGTGTTGG+TGG + tig0014103:157046-157065 None:intergenic 50.0%
!! TGCTTCAATGGCTTGGTCCA+TGG + tig0014103:157106-157125 None:intergenic 50.0%
ACAAGCGTGCCAAATCAGCA+TGG - tig0014103:157166-157185 Msa1353940:CDS 50.0%
CAAGCGTGCCAAATCAGCAT+GGG - tig0014103:157167-157186 Msa1353940:CDS 50.0%
GCCAGATAGAAAGCTGCTTC+AGG - tig0014103:157515-157534 Msa1353940:CDS 50.0%
GCCTGAAGCAGCTTTCTATC+TGG + tig0014103:157519-157538 None:intergenic 50.0%
! TGCAACAACACCCTCTGAGA+TGG + tig0014103:157613-157632 None:intergenic 50.0%
GTTGCAGCTGCTAAGCTTAG+TGG - tig0014103:157627-157646 Msa1353940:CDS 50.0%
GCTGCTAAGCTTAGTGGGAA+AGG - tig0014103:157633-157652 Msa1353940:CDS 50.0%
! GGTAGCCTTTGAAGGCACAA+CGG + tig0014103:158937-158956 None:intergenic 50.0%
! GGTGTCTTGGTAGCCTTTGA+AGG + tig0014103:158945-158964 None:intergenic 50.0%
GCGGTTGGCGTAAGCATATA+AGG + tig0014103:158966-158985 None:intergenic 50.0%
TGTTGCAGTCGCGGTAGAAT+TGG + tig0014103:158988-159007 None:intergenic 50.0%
TACCGCGACTGCAACATCTA+CGG - tig0014103:158992-159011 Msa1353940:CDS 50.0%
TTCCGTAGATGTTGCAGTCG+CGG + tig0014103:158997-159016 None:intergenic 50.0%
! GTGCTGTTACGGTGTTCTGT+TGG + tig0014103:159096-159115 None:intergenic 50.0%
CAGAACACCGTAACAGCACA+AGG - tig0014103:159097-159116 Msa1353940:CDS 50.0%
ATTCACAAGGTTGGGCTCCA+TGG - tig0014103:159287-159306 Msa1353940:CDS 50.0%
!! GTTGGTGGTGGTGCTAATAC+TGG - tig0014103:159358-159377 Msa1353940:CDS 50.0%
GGTGGAAGAGTTAATTGGCC+TGG - tig0014103:159379-159398 Msa1353940:CDS 50.0%
!! AGACAGTGTGTTGGTGGTGG+TGG + tig0014103:157040-157059 None:intergenic 55.0%
! GGTAGACAGTGTGTTGGTGG+TGG + tig0014103:157043-157062 None:intergenic 55.0%
TCTGGCCCTGAAAGCCAGTA+TGG + tig0014103:157501-157520 None:intergenic 55.0%
AAGCTGCTTCAGGCAACACC+AGG - tig0014103:157525-157544 Msa1353940:CDS 55.0%
AGCTGCTTCAGGCAACACCA+GGG - tig0014103:157526-157545 Msa1353940:CDS 55.0%
TTCAGGCAACACCAGGGTCA+GGG - tig0014103:157532-157551 Msa1353940:CDS 55.0%
GGGTCCTGCCGTATTCTCAA+AGG + tig0014103:158861-158880 None:intergenic 55.0%
CAAGCAGTAGCTCTCCGTTC+CGG - tig0014103:158890-158909 Msa1353940:CDS 55.0%
GAAAACAGAGTGATCGGCGC+CGG + tig0014103:158912-158931 None:intergenic 55.0%
TGCAGTCGCGGTAGAATTGG+CGG + tig0014103:158985-159004 None:intergenic 55.0%
ACAAGGTTGGGCTCCATGGA+TGG - tig0014103:159291-159310 Msa1353940:CDS 55.0%
CAAGGTTGGGCTCCATGGAT+GGG - tig0014103:159292-159311 Msa1353940:CDS 55.0%
! GGTGGTGGTGCTAATACTGG+TGG - tig0014103:159361-159380 Msa1353940:CDS 55.0%
!! AGTGGTGTGCCGTTTGATGC+AGG - tig0014103:159484-159503 Msa1353940:CDS 55.0%
! AGAGGATGGTGAAAGCGTGG+TGG - tig0014103:159630-159649 Msa1353940:CDS 55.0%
CTTCAGGCAACACCAGGGTC+AGG - tig0014103:157531-157550 Msa1353940:CDS 60.0%
CGGAACGGAGAGCTACTGCT+TGG + tig0014103:158892-158911 None:intergenic 60.0%
GTCGCGGTAGAATTGGCGGT+TGG + tig0014103:158981-159000 None:intergenic 60.0%
! CAGAGTGATCGGCGCCGGAA+CGG + tig0014103:158907-158926 None:intergenic 65.0%
GGTTGGGCTCCATGGATGGG+TGG - tig0014103:159295-159314 Msa1353940:CDS 65.0%
!! CTTGGTGCTGGCCCTGACCC+TGG + tig0014103:157546-157565 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0014103 gene 156811 159836 156811 ID=Msa1353940;Name=Msa1353940
tig0014103 mRNA 156811 159836 156811 ID=Msa1353940-mRNA-1;Parent=Msa1353940;Name=Msa1353940-mRNA-1;_AED=0.00;_eAED=0.00;_QI=75|1|1|1|1|1|2|323|541
tig0014103 exon 156811 157825 156811 ID=Msa1353940-mRNA-1:exon:8862;Parent=Msa1353940-mRNA-1
tig0014103 exon 158828 159836 158828 ID=Msa1353940-mRNA-1:exon:8861;Parent=Msa1353940-mRNA-1
tig0014103 five_prime_UTR 159762 159836 159762 ID=Msa1353940-mRNA-1:five_prime_utr;Parent=Msa1353940-mRNA-1
tig0014103 CDS 158828 159761 158828 ID=Msa1353940-mRNA-1:cds;Parent=Msa1353940-mRNA-1
tig0014103 CDS 157134 157825 157134 ID=Msa1353940-mRNA-1:cds;Parent=Msa1353940-mRNA-1
tig0014103 three_prime_UTR 156811 157133 156811 ID=Msa1353940-mRNA-1:three_prime_utr;Parent=Msa1353940-mRNA-1
Gene Sequence

>Msa1353940

ATGCACAATATTGCATATCCTAATATGAATAAACTCATATTCAACTCTTGCATTGCATACTTTCTCCTTTTTTCTCTCTTGTTTGTGCATGGCAAAGAGTTATCATGCAATCAAACCCCATACCCTCATGTGTGTAACCATTACATTGGAACCACCACCACCAACACACTGTCTACCCTAGATTCTTCTTCTTTCCATGATATGGCTCTTAAGGTCACCATGGACCAAGCCATTGAAGCACACAAACTTGTATCAACCATGGAATTAAATAATTTCAAAGACAAGCGTGCCAAATCAGCATGGGAAGATTGTTTAGAGCTTTATGAGGATACAATTTATCAACTCAAACGCTCAATAAACTCAAACAACCTAAATGATAAATTAACTTGGCAAAGTGCTTCCATTACCAATCATCAAACTTGTAAAAATGGTTTCATTGATTTCAACCTTCCATCTCACTTAAACTACTTCCCATCCATGTTAAGCAACTTCACTAAATTGCTTAGCAATTCTTTGTCCATTAGCAACACTCTAACTTCATCACCATCATCCTCATCCACATCATCAAGTACTAAACAAAATGGAGGGAGAAGGTTGTTATCAGATAAATTTCCATACTGGCTTTCAGGGCCAGATAGAAAGCTGCTTCAGGCAACACCAGGGTCAGGGCCAGCACCAAGAGCTGATATTGTTGTTGCACAAGATGGAAGTGGAAACTATAAGACCATCTCAGAGGGTGTTGTTGCAGCTGCTAAGCTTAGTGGGAAAGGAAGAGTTGTCATTCATGTGAAATCAGGTGTTTATAGAGAGAATGTTGATATCAAGAAGACAGTGAAGAATTTAATGATATTTGGAGATGGAATGGACTCCACTATTGTTACTGGTAATCATAATGCTATAGATGGTTCTACTACTTTTCGTTCAGCTACTTTTGCTGTTATGGGAGATGGTTTCATAGCTAAGGACATGACCTTTGAGAATACGGCAGGACCCCAAAAACACCAAGCAGTAGCTCTCCGTTCCGGCGCCGATCACTCTGTTTTCTACCGTTGTGCCTTCAAAGGCTACCAAGACACCTTATATGCTTACGCCAACCGCCAATTCTACCGCGACTGCAACATCTACGGAACTATTGATTTCATCTTCGGCAACGCAGTTACTGTCCTTCAAAATTGCAACATCTTCGTTAGAAAACCAATGAGCAACCAACAGAACACCGTAACAGCACAAGGAAGAACAGATCCAAATGAAAACACAGGAATTGTAATACATAACTGCCGTATAACCGCAGCAAATGATTTGAAACCGGTTCAGAATTCAGTTAAAACATATCTTGGTAGACCATGGCAGAAATATTCAAGAACGGTTGTAATGAAAAGTAATCTTGATGGTTTGATAAATTCACAAGGTTGGGCTCCATGGATGGGTGGTTTTGCATTGAGTACACTTTATTATGGTGAATATATGAATGTTGGTGGTGGTGCTAATACTGGTGGAAGAGTTAATTGGCCTGGTTTTCATGTTATTACTAATCCTTCTGAGGCTGTTAAATTTTCTGTTGGTAATTTTTTGGCTGGTGATTCTTGGATTTCAGGGAGTGGTGTGCCGTTTGATGCAGGTTTGTGA
Protein sequence

>Msa1353940

MHNIAYPNMNKLIFNSCIAYFLLFSLLFVHGKELSCNQTPYPHVCNHYIGTTTTNTLSTLDSSSFHDMALKVTMDQAIEAHKLVSTMELNNFKDKRAKSAWEDCLELYEDTIYQLKRSINSNNLNDKLTWQSASITNHQTCKNGFIDFNLPSHLNYFPSMLSNFTKLLSNSLSISNTLTSSPSSSSTSSSTKQNGGRRLLSDKFPYWLSGPDRKLLQATPGSGPAPRADIVVAQDGSGNYKTISEGVVAAAKLSGKGRVVIHVKSGVYRENVDIKKTVKNLMIFGDGMDSTIVTGNHNAIDGSTTFRSATFAVMGDGFIAKDMTFENTAGPQKHQAVALRSGADHSVFYRCAFKGYQDTLYAYANRQFYRDCNIYGTIDFIFGNAVTVLQNCNIFVRKPMSNQQNTVTAQGRTDPNENTGIVIHNCRITAANDLKPVQNSVKTYLGRPWQKYSRTVVMKSNLDGLINSQGWAPWMGGFALSTLYYGEYMNVGGGANTGGRVNWPGFHVITNPSEAVKFSVGNFLAGDSWISGSGVPFDAGL*