AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0710240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0710240 MtrunA17_Chr5g0412271 94.915 118 4 1 1 116 111 228 4.01e-75 222
Msa0710240 MtrunA17_Chr5g0412461 82.051 117 20 1 1 116 111 227 8.19e-64 193
Msa0710240 MtrunA17_Chr5g0427401 50.000 116 55 3 1 114 109 223 1.28e-22 88.2
Msa0710240 MtrunA17_Chr4g0049901 42.982 114 50 3 1 114 139 237 5.26e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0710240 AT2G22540.3 57.692 130 41 3 1 116 111 240 2.43e-39 132
Msa0710240 AT2G22540.1 57.692 130 41 3 1 116 111 240 2.43e-39 132
Msa0710240 AT2G22540.2 57.692 130 41 3 1 116 106 235 3.25e-39 131
Msa0710240 AT4G24540.1 46.552 116 50 4 1 113 111 217 8.09e-22 86.3

Find 91 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATTATTGTTCCTCAAAA+AGG 0.189187 5_1:-2233363 Msa0710240:five_prime_UTR
TCAATGAATGAATATGATTT+TGG 0.193776 5_1:+2233582 None:intergenic
ACATTCCTTCAGCCGGTATA+TGG 0.208025 5_1:+2229667 None:intergenic
TTGGAGAGGTCACTTGAAAT+TGG 0.219701 5_1:-2231155 Msa0710240:CDS
GAATATTTCTAACCTTTCTT+TGG 0.260465 5_1:+2230983 None:intergenic
CCCTCATCTGCCTTAATTGA+TGG 0.266278 5_1:+2231219 None:intergenic
ATCCAAAAGAGGATAAATTA+AGG 0.281250 5_1:-2232914 Msa0710240:intron
AAAGAGAGTCAGAAAGAAAA+AGG 0.293042 5_1:+2233454 None:intergenic
ATTGCTTTGCCTTACCATAA+AGG 0.320424 5_1:-2233512 Msa0710240:five_prime_UTR
GGGATTAAACTGAGTGTGTT+TGG 0.331817 5_1:-2229634 Msa0710240:three_prime_UTR
TTGCTTTGCCTTACCATAAA+GGG 0.340354 5_1:-2233511 Msa0710240:five_prime_UTR
TATCTGAACTCTCATAATCT+TGG 0.352501 5_1:+2229874 None:intergenic
CTAAGAAGAGCCATCAATTA+AGG 0.355623 5_1:-2231229 Msa0710240:five_prime_UTR
ACTGAGTGTGTTTGGATTGA+GGG 0.361899 5_1:-2229626 Msa0710240:three_prime_UTR
GAAGAGAGGATTAATCAAAA+AGG 0.364584 5_1:-2232811 Msa0710240:five_prime_UTR
TGTGGAAGAATTACAACAAT+TGG 0.373590 5_1:-2231174 Msa0710240:CDS
AGAAGAAACTGGTGATCAAA+TGG 0.377275 5_1:+2233255 None:intergenic
CTCAAAGAACCTCGCAAAGC+TGG 0.379678 5_1:-2231490 Msa0710240:five_prime_UTR
ATCTGAACTCTCATAATCTT+GGG 0.383551 5_1:+2229875 None:intergenic
TCTTTGAATACTCCAACTTA+AGG 0.399297 5_1:-2232722 Msa0710240:intron
CACCAGTTTCTTCTCTATAG+AGG 0.410201 5_1:-2233246 Msa0710240:five_prime_UTR
AGAACCGTCTCTTGAGTTGC+AGG 0.416179 5_1:-2231466 Msa0710240:intron
TGGAGAGGTCACTTGAAATT+GGG 0.419481 5_1:-2231154 Msa0710240:CDS
GACAAAAGAAAGAGAAGTGA+AGG 0.420606 5_1:+2233069 None:intergenic
TCAGATACATCGCTCAAGTT+GGG 0.436087 5_1:-2229858 Msa0710240:CDS
CATTCCTTCAGCCGGTATAT+GGG 0.442303 5_1:+2229668 None:intergenic
ATGATGGATCATAAGGTTGC+GGG 0.443250 5_1:-2230002 Msa0710240:intron
TGTGTTTGGATTGAGGGTTT+AGG 0.443773 5_1:-2229620 Msa0710240:three_prime_UTR
ACTCATACCCTAGTGACCTT+TGG 0.446181 5_1:-2233551 Msa0710240:five_prime_UTR
TTTGGATTGAGGGTTTAGGA+GGG 0.447816 5_1:-2229616 Msa0710240:three_prime_UTR
GATGCAGTCATGCAGGCATT+TGG 0.450177 5_1:+2233149 None:intergenic
GTCTCTGAAAGATAATGAAA+TGG 0.452122 5_1:+2233200 None:intergenic
AAATTAAGGTAAAGAAGAAA+TGG 0.460280 5_1:-2232900 Msa0710240:five_prime_UTR
CTCGTCTTCCAAAGGTCACT+AGG 0.464850 5_1:+2233543 None:intergenic
GAGAAAGAAAGACCTTAAGT+TGG 0.470112 5_1:+2232710 None:intergenic
AGGTCACTTGAAATTGGGTT+GGG 0.472406 5_1:-2231149 Msa0710240:CDS
GTTTGGATTGAGGGTTTAGG+AGG 0.475751 5_1:-2229617 Msa0710240:three_prime_UTR
TTCAGATACATCGCTCAAGT+TGG 0.479254 5_1:-2229859 Msa0710240:CDS
GAGGTCACTTGAAATTGGGT+TGG 0.488246 5_1:-2231150 Msa0710240:CDS
GATCAATGATCTCCAAAGAA+AGG 0.499633 5_1:-2230995 Msa0710240:intron
ATCCTCTATAGAGAAGAAAC+TGG 0.502434 5_1:+2233244 None:intergenic
GTAGTGTCAAATGTACTTTG+TGG 0.504405 5_1:+2233330 None:intergenic
AGATCTTGAAGGTTTGAATG+TGG 0.507969 5_1:-2231192 Msa0710240:CDS
AAGTAAACCCCTTTATGGTA+AGG 0.510438 5_1:+2233503 None:intergenic
AACTGAGTGTGTTTGGATTG+AGG 0.511779 5_1:-2229627 Msa0710240:three_prime_UTR
AGATGTTTATTTGGTTAGTG+AGG 0.512510 5_1:+2233399 None:intergenic
CTCTATAGAGGATCTAAAAG+AGG 0.520099 5_1:-2233234 Msa0710240:five_prime_UTR
ATTTGCAGGCTCCCATATAC+CGG 0.525181 5_1:-2229679 Msa0710240:intron
TGGATGAATTATGGGTTAGG+AGG 0.527557 5_1:+2233298 None:intergenic
ATCATACATGTTGCTTTAGT+CGG 0.530014 5_1:+2230190 None:intergenic
ATAATCTTGGGGAGGACCAA+TGG 0.530085 5_1:+2229887 None:intergenic
GAAGAAACTGGTGATCAAAT+GGG 0.531162 5_1:+2233256 None:intergenic
ATGAGGGGAGAAGATCTTGA+AGG 0.541103 5_1:-2231203 Msa0710240:CDS
CCATCAATTAAGGCAGATGA+GGG 0.555256 5_1:-2231219 Msa0710240:exon
TATGAGAATGTGGTTAATGA+AGG 0.563013 5_1:-2229945 Msa0710240:CDS
ATGAAATGATGCAGTCATGC+AGG 0.563073 5_1:+2233142 None:intergenic
TCAGGGAGAGAAGATCATGA+TGG 0.564270 5_1:-2231019 Msa0710240:intron
ATGATTAACGACAGAATGGT+TGG 0.571480 5_1:-2229978 Msa0710240:CDS
GCCATCAATTAAGGCAGATG+AGG 0.573391 5_1:-2231220 Msa0710240:exon
CACACAAACATTCCTTCAGC+CGG 0.574541 5_1:+2229660 None:intergenic
ACCAACGTTTACAACTCCAT+TGG 0.576740 5_1:-2229903 Msa0710240:CDS
GGGCATGATTAACGACAGAA+TGG 0.581587 5_1:-2229982 Msa0710240:CDS
CCTGCAGGGAAGGCAACTGA+TGG 0.581631 5_1:-2230223 Msa0710240:intron
ATAGAGGATCTAAAAGAGGA+AGG 0.586408 5_1:-2233230 Msa0710240:five_prime_UTR
TTGAGGAACAATAATGAACA+TGG 0.587700 5_1:+2233368 None:intergenic
AACGTTGGTGACAGATTCTG+AGG 0.592708 5_1:+2229917 None:intergenic
TGCTTTGCCTTACCATAAAG+GGG 0.597645 5_1:-2233510 Msa0710240:five_prime_UTR
GTGGCTCGCTCGTCTTCCAA+AGG 0.597700 5_1:+2233535 None:intergenic
TGAAGGAATGTTTGTGTGCG+AGG 0.598854 5_1:-2229655 Msa0710240:three_prime_UTR
CAGATACATCGCTCAAGTTG+GGG 0.603531 5_1:-2229857 Msa0710240:intron
ACGGTTCTTCCAGCTTTGCG+AGG 0.603660 5_1:+2231481 None:intergenic
TCGTCTTCCAAAGGTCACTA+GGG 0.607418 5_1:+2233544 None:intergenic
GCGTGAGGGAAATACTTGAA+AGG 0.613182 5_1:-2231524 Msa0710240:five_prime_UTR
TTGGATTGAGGGTTTAGGAG+GGG 0.613941 5_1:-2229615 Msa0710240:three_prime_UTR
TGTGTTATGATGAAGCGTGA+GGG 0.617556 5_1:-2231538 Msa0710240:intron
TCTGAACTCTCATAATCTTG+GGG 0.621502 5_1:+2229876 None:intergenic
GGGCCGTGTAATAGAAAACA+AGG 0.627922 5_1:-2231129 Msa0710240:intron
AAGAATTACAACAATTGGAG+AGG 0.631162 5_1:-2231169 Msa0710240:CDS
GGCTCCCATATACCGGCTGA+AGG 0.633026 5_1:-2229672 Msa0710240:exon
GAAGGAATGTTTGTGTGCGA+GGG 0.633057 5_1:-2229654 Msa0710240:three_prime_UTR
ATTAACGACAGAATGGTTGG+AGG 0.640229 5_1:-2229975 Msa0710240:CDS
CAACTGTTCTAGATTGAGCA+AGG 0.640451 5_1:-2231258 Msa0710240:five_prime_UTR
CCAACCTGCAACTCAAGAGA+CGG 0.649927 5_1:+2231462 None:intergenic
ACCAATGGAGTTGTAAACGT+TGG 0.652128 5_1:+2229902 None:intergenic
AGGTGATGAATATGAGAATG+TGG 0.655081 5_1:-2229955 Msa0710240:CDS
GAACTCTCATAATCTTGGGG+AGG 0.658116 5_1:+2229879 None:intergenic
AGATTGAAAACGCAACGGCG+AGG 0.667280 5_1:-2232853 Msa0710240:five_prime_UTR
GAAACTGGTGATCAAATGGG+TGG 0.670041 5_1:+2233259 None:intergenic
AAAGAAGATTGAAAACGCAA+CGG 0.680027 5_1:-2232858 Msa0710240:five_prime_UTR
TATGGTAAGGCAAAGCAATG+TGG 0.708560 5_1:+2233516 None:intergenic
CATCAATTAAGGCAGATGAG+GGG 0.712596 5_1:-2231218 Msa0710240:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACAAAAGTTTAAAAAT+AGG - chr5_1:2232339-2232358 Msa0710240:intron 10.0%
!! TGTATTTGTAAAAAAAAAAT+TGG - chr5_1:2232372-2232391 Msa0710240:intron 10.0%
!!! TTTATTTTTGGATGAATTAT+GGG + chr5_1:2229751-2229770 None:intergenic 15.0%
!!! ATTTATTTTTGGATGAATTA+TGG + chr5_1:2229752-2229771 None:intergenic 15.0%
!! ATATATGTAGCAAAAAATAA+AGG - chr5_1:2230069-2230088 Msa0710240:intron 15.0%
!!! AAGGAAAAAAAAATAGATTT+TGG - chr5_1:2231207-2231226 Msa0710240:exon 15.0%
!!! ATCATTCATTGTTATTTATT+TGG - chr5_1:2231457-2231476 Msa0710240:intron 15.0%
!!! AATGTTTTATTTGTTCATTT+TGG + chr5_1:2231665-2231684 None:intergenic 15.0%
!!! AAAAAAAAAAAAGTACTAGT+AGG + chr5_1:2231947-2231966 None:intergenic 15.0%
!!! AAAATATTGTTTTGTTTTTC+AGG - chr5_1:2232001-2232020 Msa0710240:intron 15.0%
!!! AAATATTGTTTTGTTTTTCA+GGG - chr5_1:2232002-2232021 Msa0710240:intron 15.0%
!! GACTAAAATTTAATTAGATA+GGG - chr5_1:2232109-2232128 Msa0710240:intron 15.0%
!!! TTTTTTTTTAACAATCAAAG+TGG + chr5_1:2232220-2232239 None:intergenic 15.0%
!!! TACTTTTACTAAAAATTGTA+AGG - chr5_1:2232249-2232268 Msa0710240:intron 15.0%
!!! ACTTTTACTAAAAATTGTAA+GGG - chr5_1:2232250-2232269 Msa0710240:intron 15.0%
!! AAAAATATCATGCAAATATT+GGG + chr5_1:2232968-2232987 None:intergenic 15.0%
!! TAAAAATATCATGCAAATAT+TGG + chr5_1:2232969-2232988 None:intergenic 15.0%
!! AAAAAATTACTTGTATATGA+TGG - chr5_1:2233020-2233039 Msa0710240:intron 15.0%
!!! GTGGTTTCTATATTTATTTT+TGG + chr5_1:2229763-2229782 None:intergenic 20.0%
!!! ATTATTGTTTTATCCAAAAG+AGG - chr5_1:2230113-2230132 Msa0710240:intron 20.0%
!! AAATTAAGGTAAAGAAGAAA+TGG - chr5_1:2230138-2230157 Msa0710240:intron 20.0%
!! ACTCTAATGAATTAAAACAA+TGG - chr5_1:2230376-2230395 Msa0710240:intron 20.0%
!!! GTAGATCTTTTTTCAAAAAA+TGG - chr5_1:2230468-2230487 Msa0710240:intron 20.0%
!!! CTTTTTTCAAAAAATGGTAA+AGG - chr5_1:2230474-2230493 Msa0710240:intron 20.0%
!!! AAAAACAAAGTTCTAAACAA+AGG + chr5_1:2230631-2230650 None:intergenic 20.0%
!! TTTATCCTAATAAGTGAAAT+CGG - chr5_1:2230777-2230796 Msa0710240:intron 20.0%
!! GTAAGAGTTAGATTAAAATA+TGG + chr5_1:2230842-2230861 None:intergenic 20.0%
!! TTTATATAGCTAATGTTACA+CGG - chr5_1:2231062-2231081 Msa0710240:intron 20.0%
!! TAAATTAATTGTTGTCATGT+TGG - chr5_1:2231093-2231112 Msa0710240:intron 20.0%
!! AAATTAATTGTTGTCATGTT+GGG - chr5_1:2231094-2231113 Msa0710240:intron 20.0%
!!! AGGAATATCTTTTTATTTTG+AGG - chr5_1:2231240-2231259 Msa0710240:five_prime_UTR 20.0%
!!! TGATCTTTTTTTTAACAAGA+TGG - chr5_1:2231270-2231289 Msa0710240:five_prime_UTR 20.0%
!!! GATCTTTTTTTTAACAAGAT+GGG - chr5_1:2231271-2231290 Msa0710240:five_prime_UTR 20.0%
!! TTATTTGGTATAATTTGACT+TGG - chr5_1:2231472-2231491 Msa0710240:five_prime_UTR 20.0%
!! ACTCAACATGTTATATTAAT+TGG - chr5_1:2232084-2232103 Msa0710240:intron 20.0%
!! CGACTAAAATTTAATTAGAT+AGG - chr5_1:2232108-2232127 Msa0710240:intron 20.0%
!! ACACAATTTAATGTATGAAT+TGG + chr5_1:2232313-2232332 None:intergenic 20.0%
!!! AATTATGCTTAGTTTTGTAT+TGG - chr5_1:2232690-2232709 Msa0710240:intron 20.0%
!! TAAGTGAACTAATAATAATG+TGG + chr5_1:2233224-2233243 None:intergenic 20.0%
!!! AATGTAGAATTTCTTTTTGA+AGG - chr5_1:2233261-2233280 Msa0710240:five_prime_UTR 20.0%
!!! AAAAAACATTTTACATCGTT+CGG + chr5_1:2233474-2233493 None:intergenic 20.0%
!!! GATTTTTGGAGATGTTTATT+TGG + chr5_1:2229651-2229670 None:intergenic 25.0%
!! CAATAATGAACATGGATTTT+TGG + chr5_1:2229665-2229684 None:intergenic 25.0%
! TTCATTATTGTTCCTCAAAA+AGG - chr5_1:2229675-2229694 Msa0710240:intron 25.0%
!! TCTTTTGTCTCTTCTTTTTT+AGG - chr5_1:2229983-2230002 Msa0710240:CDS 25.0%
! ATCCAAAAGAGGATAAATTA+AGG - chr5_1:2230124-2230143 Msa0710240:intron 25.0%
!! TACCTTAATTTATCCTCTTT+TGG + chr5_1:2230129-2230148 None:intergenic 25.0%
! TTAAAAGTGAAAACAAACAC+AGG - chr5_1:2230528-2230547 Msa0710240:intron 25.0%
! TATTTCAGCCAACATTAATT+AGG - chr5_1:2230663-2230682 Msa0710240:intron 25.0%
! CCAATTATCCTAATTAATGT+TGG + chr5_1:2230674-2230693 None:intergenic 25.0%
! CCAACATTAATTAGGATAAT+TGG - chr5_1:2230671-2230690 Msa0710240:intron 25.0%
! TCACTTATTAGGATAAAGTT+TGG + chr5_1:2230774-2230793 None:intergenic 25.0%
!! TATAACCGATTTCACTTATT+AGG + chr5_1:2230785-2230804 None:intergenic 25.0%
!! GTTTTGTGTGAATTTAAATC+TGG - chr5_1:2230902-2230921 Msa0710240:intron 25.0%
! AATTAATTGTTGTCATGTTG+GGG - chr5_1:2231095-2231114 Msa0710240:intron 25.0%
!!! TAGATTTTGGAAAAGCTATT+AGG - chr5_1:2231220-2231239 Msa0710240:exon 25.0%
! TCAAAAAAGTTCCAATCAAA+CGG - chr5_1:2231406-2231425 Msa0710240:intron 25.0%
!! GAATATTTCTAACCTTTCTT+TGG + chr5_1:2232058-2232077 None:intergenic 25.0%
!!! AGATTTGTGAACAACTTTTT+TGG + chr5_1:2232474-2232493 None:intergenic 25.0%
! TTCACAAATCTTAATTCAAC+TGG - chr5_1:2232483-2232502 Msa0710240:intron 25.0%
!! GACGTCAATATTAACAATTT+TGG + chr5_1:2232514-2232533 None:intergenic 25.0%
! GAAGCTAATTTAGATATATG+TGG + chr5_1:2232770-2232789 None:intergenic 25.0%
GAAAAAAGTAAACCCCTTTA+TGG + chr5_1:2229543-2229562 None:intergenic 30.0%
AAAGAGAGTCAGAAAGAAAA+AGG + chr5_1:2229587-2229606 None:intergenic 30.0%
AGATGTTTATTTGGTTAGTG+AGG + chr5_1:2229642-2229661 None:intergenic 30.0%
TTGAGGAACAATAATGAACA+TGG + chr5_1:2229673-2229692 None:intergenic 30.0%
! GGATTTTACTTTCCTTTTTG+AGG + chr5_1:2229690-2229709 None:intergenic 30.0%
! TTTTGGATGAATTATGGGTT+AGG + chr5_1:2229746-2229765 None:intergenic 30.0%
! GTCTCTGAAAGATAATGAAA+TGG + chr5_1:2229841-2229860 None:intergenic 30.0%
AAAGAAGATTGAAAACGCAA+CGG - chr5_1:2230180-2230199 Msa0710240:intron 30.0%
GAAGAGAGGATTAATCAAAA+AGG - chr5_1:2230227-2230246 Msa0710240:intron 30.0%
! TCATCATTTTCTCTTCTACT+GGG - chr5_1:2230289-2230308 Msa0710240:intron 30.0%
TCTTTGAATACTCCAACTTA+AGG - chr5_1:2230316-2230335 Msa0710240:intron 30.0%
TTCTCTCATGAATGAAAACA+CGG - chr5_1:2230346-2230365 Msa0710240:intron 30.0%
! TTCACACAAAACACATTTTG+AGG + chr5_1:2230894-2230913 None:intergenic 30.0%
ACATATAAACCTAAACCACT+TGG - chr5_1:2230998-2231017 Msa0710240:CDS 30.0%
! CTTCTAAAAACAAAGAACAC+AGG + chr5_1:2231190-2231209 None:intergenic 30.0%
!!! CTGTGTTCTTTGTTTTTAGA+AGG - chr5_1:2231188-2231207 Msa0710240:CDS 30.0%
CAAAAAAGTTCCAATCAAAC+GGG - chr5_1:2231407-2231426 Msa0710240:intron 30.0%
TGTGGAAGAATTACAACAAT+TGG - chr5_1:2231864-2231883 Msa0710240:intron 30.0%
AAGAATTACAACAATTGGAG+AGG - chr5_1:2231869-2231888 Msa0710240:intron 30.0%
!!! AGACCTTGTTTTCTATTACA+CGG + chr5_1:2231915-2231934 None:intergenic 30.0%
ATTTAATTAGATAGGGTCGA+TGG - chr5_1:2232116-2232135 Msa0710240:intron 30.0%
TCTAAAAAATGTTCCAAGGA+TGG + chr5_1:2232277-2232296 None:intergenic 30.0%
!!! ATCCTTGGAACATTTTTTAG+AGG - chr5_1:2232276-2232295 Msa0710240:intron 30.0%
!!! TTTGTGAACAACTTTTTTGG+TGG + chr5_1:2232471-2232490 None:intergenic 30.0%
!!! CTGTTGTTTTAGAAGATAGA+TGG + chr5_1:2232604-2232623 None:intergenic 30.0%
!! ATCATACATGTTGCTTTAGT+CGG + chr5_1:2232851-2232870 None:intergenic 30.0%
!! CTTGTATATGATGGATCATA+AGG - chr5_1:2233029-2233048 Msa0710240:intron 30.0%
TATGAGAATGTGGTTAATGA+AGG - chr5_1:2233093-2233112 Msa0710240:five_prime_UTR 30.0%
ATCTGAACTCTCATAATCTT+GGG + chr5_1:2233166-2233185 None:intergenic 30.0%
TATCTGAACTCTCATAATCT+TGG + chr5_1:2233167-2233186 None:intergenic 30.0%
ATTGCTTTGCCTTACCATAA+AGG - chr5_1:2229526-2229545 Msa0710240:three_prime_UTR 35.0%
TTGCTTTGCCTTACCATAAA+GGG - chr5_1:2229527-2229546 Msa0710240:three_prime_UTR 35.0%
AAGTAAACCCCTTTATGGTA+AGG + chr5_1:2229538-2229557 None:intergenic 35.0%
AAGAAAAAGGTTGTGTGTGT+GGG + chr5_1:2229574-2229593 None:intergenic 35.0%
AAAGAAAAAGGTTGTGTGTG+TGG + chr5_1:2229575-2229594 None:intergenic 35.0%
! CTTTCTGACTCTCTTTTCAT+TGG - chr5_1:2229591-2229610 Msa0710240:three_prime_UTR 35.0%
GTAGTGTCAAATGTACTTTG+TGG + chr5_1:2229711-2229730 None:intergenic 35.0%
GAAGAAACTGGTGATCAAAT+GGG + chr5_1:2229785-2229804 None:intergenic 35.0%
AGAAGAAACTGGTGATCAAA+TGG + chr5_1:2229786-2229805 None:intergenic 35.0%
ATCCTCTATAGAGAAGAAAC+TGG + chr5_1:2229797-2229816 None:intergenic 35.0%
CTCTATAGAGGATCTAAAAG+AGG - chr5_1:2229804-2229823 Msa0710240:intron 35.0%
ATAGAGGATCTAAAAGAGGA+AGG - chr5_1:2229808-2229827 Msa0710240:intron 35.0%
GACAAAAGAAAGAGAAGTGA+AGG + chr5_1:2229972-2229991 None:intergenic 35.0%
!!! GACTTTTTCAAAGAGAAGAG+AGG - chr5_1:2230213-2230232 Msa0710240:CDS 35.0%
! CTCATCATTTTCTCTTCTAC+TGG - chr5_1:2230288-2230307 Msa0710240:intron 35.0%
GAGAAAGAAAGACCTTAAGT+TGG + chr5_1:2230331-2230350 None:intergenic 35.0%
CAAAGGATTATGCACAACAT+AGG + chr5_1:2230614-2230633 None:intergenic 35.0%
CTAAATCACTGATCAGAGTT+GGG + chr5_1:2230699-2230718 None:intergenic 35.0%
ACTAAATCACTGATCAGAGT+TGG + chr5_1:2230700-2230719 None:intergenic 35.0%
! CAATTGCACTGAAGAGTTTT+AGG + chr5_1:2230732-2230751 None:intergenic 35.0%
!! CACAAAACACATTTTGAGGA+GGG + chr5_1:2230890-2230909 None:intergenic 35.0%
!! ACACAAAACACATTTTGAGG+AGG + chr5_1:2230891-2230910 None:intergenic 35.0%
CATGATCGATGTCAACTTAA+GGG + chr5_1:2231433-2231452 None:intergenic 35.0%
CTAAGAAGAGCCATCAATTA+AGG - chr5_1:2231809-2231828 Msa0710240:intron 35.0%
AGATCTTGAAGGTTTGAATG+TGG - chr5_1:2231846-2231865 Msa0710240:intron 35.0%
GATCAATGATCTCCAAAGAA+AGG - chr5_1:2232043-2232062 Msa0710240:intron 35.0%
! GACCTCTAAAAAATGTTCCA+AGG + chr5_1:2232281-2232300 None:intergenic 35.0%
!! GTGTGTTTGAGTTTTCTAGT+CGG - chr5_1:2232427-2232446 Msa0710240:intron 35.0%
AAAATTCACACACACAAGTG+GGG + chr5_1:2232563-2232582 None:intergenic 35.0%
GAAAATTCACACACACAAGT+GGG + chr5_1:2232564-2232583 None:intergenic 35.0%
TGAAAATTCACACACACAAG+TGG + chr5_1:2232565-2232584 None:intergenic 35.0%
GTTCAGTAAATAAGGGTTGT+AGG + chr5_1:2232796-2232815 None:intergenic 35.0%
!! TATGATGGATCATAAGGTTG+CGG - chr5_1:2233035-2233054 Msa0710240:intron 35.0%
ATGATTAACGACAGAATGGT+TGG - chr5_1:2233060-2233079 Msa0710240:five_prime_UTR 35.0%
AGGTGATGAATATGAGAATG+TGG - chr5_1:2233083-2233102 Msa0710240:five_prime_UTR 35.0%
TCTGAACTCTCATAATCTTG+GGG + chr5_1:2233165-2233184 None:intergenic 35.0%
TATGGTAAGGCAAAGCAATG+TGG + chr5_1:2229525-2229544 None:intergenic 40.0%
TGCTTTGCCTTACCATAAAG+GGG - chr5_1:2229528-2229547 Msa0710240:three_prime_UTR 40.0%
AGAAAAAGGTTGTGTGTGTG+GGG + chr5_1:2229573-2229592 None:intergenic 40.0%
! TCTGACTCTCTTTTCATTGG+TGG - chr5_1:2229594-2229613 Msa0710240:three_prime_UTR 40.0%
!! TGGATGAATTATGGGTTAGG+AGG + chr5_1:2229743-2229762 None:intergenic 40.0%
CACCAGTTTCTTCTCTATAG+AGG - chr5_1:2229792-2229811 Msa0710240:intron 40.0%
ATGAAATGATGCAGTCATGC+AGG + chr5_1:2229899-2229918 None:intergenic 40.0%
TCTCTCTTTCAACTATACGC+TGG - chr5_1:2230017-2230036 Msa0710240:intron 40.0%
!! AAACACATTTTGAGGAGGGA+CGG + chr5_1:2230886-2230905 None:intergenic 40.0%
AGAGTGGTTGAATTGGACAA+GGG + chr5_1:2230960-2230979 None:intergenic 40.0%
TCCAATTCAACCACTCTCAT+AGG - chr5_1:2230963-2230982 Msa0710240:intron 40.0%
TCCTATGAGAGTGGTTGAAT+TGG + chr5_1:2230967-2230986 None:intergenic 40.0%
TCTGTACTATCCTATGAGAG+TGG + chr5_1:2230976-2230995 None:intergenic 40.0%
TTATGTGCACCAAGTGGTTT+AGG + chr5_1:2231010-2231029 None:intergenic 40.0%
GCATTATTATGTGCACCAAG+TGG + chr5_1:2231016-2231035 None:intergenic 40.0%
ATCCATGTGAACATTGCACA+GGG + chr5_1:2231038-2231057 None:intergenic 40.0%
TATCCATGTGAACATTGCAC+AGG + chr5_1:2231039-2231058 None:intergenic 40.0%
GCATGATCGATGTCAACTTA+AGG + chr5_1:2231434-2231453 None:intergenic 40.0%
TTGTGTTATGATGAAGCGTG+AGG - chr5_1:2231499-2231518 Msa0710240:five_prime_UTR 40.0%
TGTGTTATGATGAAGCGTGA+GGG - chr5_1:2231500-2231519 Msa0710240:five_prime_UTR 40.0%
! CAACTGTTCTAGATTGAGCA+AGG - chr5_1:2231780-2231799 Msa0710240:intron 40.0%
CCATCAATTAAGGCAGATGA+GGG - chr5_1:2231819-2231838 Msa0710240:intron 40.0%
CATCAATTAAGGCAGATGAG+GGG - chr5_1:2231820-2231839 Msa0710240:intron 40.0%
TTGGAGAGGTCACTTGAAAT+TGG - chr5_1:2231883-2231902 Msa0710240:intron 40.0%
TGGAGAGGTCACTTGAAATT+GGG - chr5_1:2231884-2231903 Msa0710240:intron 40.0%
AGGTCACTTGAAATTGGGTT+GGG - chr5_1:2231889-2231908 Msa0710240:intron 40.0%
TAATTAGATAGGGTCGATGG+AGG - chr5_1:2232119-2232138 Msa0710240:intron 40.0%
AAATTGTAAGGGTCCATCCT+TGG - chr5_1:2232261-2232280 Msa0710240:intron 40.0%
CCTGCAGGTTCAGTAAATAA+GGG + chr5_1:2232803-2232822 None:intergenic 40.0%
! CCTTATTTACTGAACCTGCA+GGG - chr5_1:2232801-2232820 Msa0710240:five_prime_UTR 40.0%
!! ATGATGGATCATAAGGTTGC+GGG - chr5_1:2233036-2233055 Msa0710240:intron 40.0%
ATTAACGACAGAATGGTTGG+AGG - chr5_1:2233063-2233082 Msa0710240:five_prime_UTR 40.0%
ACCAACGTTTACAACTCCAT+TGG - chr5_1:2233135-2233154 Msa0710240:five_prime_UTR 40.0%
ACCAATGGAGTTGTAAACGT+TGG + chr5_1:2233139-2233158 None:intergenic 40.0%
TTCAGATACATCGCTCAAGT+TGG - chr5_1:2233179-2233198 Msa0710240:five_prime_UTR 40.0%
TCAGATACATCGCTCAAGTT+GGG - chr5_1:2233180-2233199 Msa0710240:five_prime_UTR 40.0%
CGCTTTCATGTACTATTTGC+AGG - chr5_1:2233345-2233364 Msa0710240:five_prime_UTR 40.0%
GGGATTAAACTGAGTGTGTT+TGG - chr5_1:2233404-2233423 Msa0710240:five_prime_UTR 40.0%
AACTGAGTGTGTTTGGATTG+AGG - chr5_1:2233411-2233430 Msa0710240:five_prime_UTR 40.0%
ACTGAGTGTGTTTGGATTGA+GGG - chr5_1:2233412-2233431 Msa0710240:five_prime_UTR 40.0%
TGTGTTTGGATTGAGGGTTT+AGG - chr5_1:2233418-2233437 Msa0710240:five_prime_UTR 40.0%
TTTGGATTGAGGGTTTAGGA+GGG - chr5_1:2233422-2233441 Msa0710240:five_prime_UTR 40.0%
ACTCATACCCTAGTGACCTT+TGG - chr5_1:2229487-2229506 Msa0710240:three_prime_UTR 45.0%
TCGTCTTCCAAAGGTCACTA+GGG + chr5_1:2229497-2229516 None:intergenic 45.0%
GAAACTGGTGATCAAATGGG+TGG + chr5_1:2229782-2229801 None:intergenic 45.0%
!!! GCACTGAAGAGTTTTAGGTG+TGG + chr5_1:2230727-2230746 None:intergenic 45.0%
GAGAGTGGTTGAATTGGACA+AGG + chr5_1:2230961-2230980 None:intergenic 45.0%
AACTTAAGGGCCCGTTTGAT+TGG + chr5_1:2231420-2231439 None:intergenic 45.0%
GCGTGAGGGAAATACTTGAA+AGG - chr5_1:2231514-2231533 Msa0710240:five_prime_UTR 45.0%
GCCATCAATTAAGGCAGATG+AGG - chr5_1:2231818-2231837 Msa0710240:intron 45.0%
CCCTCATCTGCCTTAATTGA+TGG + chr5_1:2231822-2231841 None:intergenic 45.0%
ATGAGGGGAGAAGATCTTGA+AGG - chr5_1:2231835-2231854 Msa0710240:intron 45.0%
GAGGTCACTTGAAATTGGGT+TGG - chr5_1:2231888-2231907 Msa0710240:intron 45.0%
GGGCCGTGTAATAGAAAACA+AGG - chr5_1:2231909-2231928 Msa0710240:intron 45.0%
TCAGGGAGAGAAGATCATGA+TGG - chr5_1:2232019-2232038 Msa0710240:intron 45.0%
! CCCTTATTTACTGAACCTGC+AGG - chr5_1:2232800-2232819 Msa0710240:five_prime_UTR 45.0%
CCCTGCAGGTTCAGTAAATA+AGG + chr5_1:2232804-2232823 None:intergenic 45.0%
ATTTACTGAACCTGCAGGGA+AGG - chr5_1:2232805-2232824 Msa0710240:five_prime_UTR 45.0%
GGGCATGATTAACGACAGAA+TGG - chr5_1:2233056-2233075 Msa0710240:five_prime_UTR 45.0%
!! AACGTTGGTGACAGATTCTG+AGG + chr5_1:2233124-2233143 None:intergenic 45.0%
ATAATCTTGGGGAGGACCAA+TGG + chr5_1:2233154-2233173 None:intergenic 45.0%
GAACTCTCATAATCTTGGGG+AGG + chr5_1:2233162-2233181 None:intergenic 45.0%
! CAGATACATCGCTCAAGTTG+GGG - chr5_1:2233181-2233200 Msa0710240:five_prime_UTR 45.0%
ATTTGCAGGCTCCCATATAC+CGG - chr5_1:2233359-2233378 Msa0710240:five_prime_UTR 45.0%
CATTCCTTCAGCCGGTATAT+GGG + chr5_1:2233373-2233392 None:intergenic 45.0%
ACATTCCTTCAGCCGGTATA+TGG + chr5_1:2233374-2233393 None:intergenic 45.0%
CACACAAACATTCCTTCAGC+CGG + chr5_1:2233381-2233400 None:intergenic 45.0%
TGAAGGAATGTTTGTGTGCG+AGG - chr5_1:2233383-2233402 Msa0710240:five_prime_UTR 45.0%
GAAGGAATGTTTGTGTGCGA+GGG - chr5_1:2233384-2233403 Msa0710240:five_prime_UTR 45.0%
GTTTGGATTGAGGGTTTAGG+AGG - chr5_1:2233421-2233440 Msa0710240:five_prime_UTR 45.0%
TTGGATTGAGGGTTTAGGAG+GGG - chr5_1:2233423-2233442 Msa0710240:five_prime_UTR 45.0%
!!! ATTTTTTAAATTTCTAAAAA+TGG - chr5_1:2230446-2230465 Msa0710240:intron 5.0%
CTCGTCTTCCAAAGGTCACT+AGG + chr5_1:2229498-2229517 None:intergenic 50.0%
GATGCAGTCATGCAGGCATT+TGG + chr5_1:2229892-2229911 None:intergenic 50.0%
AGATTGAAAACGCAACGGCG+AGG - chr5_1:2230185-2230204 Msa0710240:intron 50.0%
!! TTTTGAGGAGGGACGGACAT+AGG + chr5_1:2230879-2230898 None:intergenic 50.0%
GACGCGATGAGCTTTACAAG+CGG + chr5_1:2230938-2230957 None:intergenic 50.0%
TGCCCTGTGCAATGTTCACA+TGG - chr5_1:2231033-2231052 Msa0710240:intron 50.0%
CTCAAAGAACCTCGCAAAGC+TGG - chr5_1:2231548-2231567 Msa0710240:intron 50.0%
! AGAACCGTCTCTTGAGTTGC+AGG - chr5_1:2231572-2231591 Msa0710240:intron 50.0%
CCAACCTGCAACTCAAGAGA+CGG + chr5_1:2231579-2231598 None:intergenic 50.0%
ACACACAAGTGGGGAATCTC+TGG + chr5_1:2232554-2232573 None:intergenic 50.0%
!! ATACATCGCTCAAGTTGGGG+TGG - chr5_1:2233184-2233203 Msa0710240:five_prime_UTR 50.0%
!! TACATCGCTCAAGTTGGGGT+GGG - chr5_1:2233185-2233204 Msa0710240:five_prime_UTR 50.0%
ACGGTTCTTCCAGCTTTGCG+AGG + chr5_1:2231560-2231579 None:intergenic 55.0%
! CCGTCTCTTGAGTTGCAGGT+TGG - chr5_1:2231576-2231595 Msa0710240:intron 55.0%
GTGGCTCGCTCGTCTTCCAA+AGG + chr5_1:2229506-2229525 None:intergenic 60.0%
CCATCAGTTGCCTTCCCTGC+AGG + chr5_1:2232818-2232837 None:intergenic 60.0%
CCTGCAGGGAAGGCAACTGA+TGG - chr5_1:2232815-2232834 Msa0710240:five_prime_UTR 60.0%
! GGCTCCCATATACCGGCTGA+AGG - chr5_1:2233366-2233385 Msa0710240:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 2229457 2233603 2229457 ID=Msa0710240;Name=Msa0710240
chr5_1 mRNA 2229457 2233603 2229457 ID=Msa0710240-mRNA-1;Parent=Msa0710240;Name=Msa0710240-mRNA-1;_AED=0.13;_eAED=0.13;_QI=901|1|1|1|0.57|0.62|8|218|116
chr5_1 exon 2229457 2229693 2229457 ID=Msa0710240-mRNA-1:exon:677;Parent=Msa0710240-mRNA-1
chr5_1 exon 2229858 2230009 2229858 ID=Msa0710240-mRNA-1:exon:676;Parent=Msa0710240-mRNA-1
chr5_1 exon 2230197 2230238 2230197 ID=Msa0710240-mRNA-1:exon:675;Parent=Msa0710240-mRNA-1
chr5_1 exon 2230996 2231037 2230996 ID=Msa0710240-mRNA-1:exon:674;Parent=Msa0710240-mRNA-1
chr5_1 exon 2231130 2231294 2231130 ID=Msa0710240-mRNA-1:exon:673;Parent=Msa0710240-mRNA-1
chr5_1 exon 2231467 2231545 2231467 ID=Msa0710240-mRNA-1:exon:672;Parent=Msa0710240-mRNA-1
chr5_1 exon 2232723 2232927 2232723 ID=Msa0710240-mRNA-1:exon:671;Parent=Msa0710240-mRNA-1
chr5_1 exon 2233056 2233603 2233056 ID=Msa0710240-mRNA-1:exon:670;Parent=Msa0710240-mRNA-1
chr5_1 five_prime_UTR 2233056 2233603 2233056 ID=Msa0710240-mRNA-1:five_prime_utr;Parent=Msa0710240-mRNA-1
chr5_1 five_prime_UTR 2232723 2232927 2232723 ID=Msa0710240-mRNA-1:five_prime_utr;Parent=Msa0710240-mRNA-1
chr5_1 five_prime_UTR 2231467 2231545 2231467 ID=Msa0710240-mRNA-1:five_prime_utr;Parent=Msa0710240-mRNA-1
chr5_1 five_prime_UTR 2231226 2231294 2231226 ID=Msa0710240-mRNA-1:five_prime_utr;Parent=Msa0710240-mRNA-1
chr5_1 CDS 2231130 2231225 2231130 ID=Msa0710240-mRNA-1:cds;Parent=Msa0710240-mRNA-1
chr5_1 CDS 2230996 2231037 2230996 ID=Msa0710240-mRNA-1:cds;Parent=Msa0710240-mRNA-1
chr5_1 CDS 2230197 2230238 2230197 ID=Msa0710240-mRNA-1:cds;Parent=Msa0710240-mRNA-1
chr5_1 CDS 2229858 2230009 2229858 ID=Msa0710240-mRNA-1:cds;Parent=Msa0710240-mRNA-1
chr5_1 CDS 2229675 2229693 2229675 ID=Msa0710240-mRNA-1:cds;Parent=Msa0710240-mRNA-1
chr5_1 three_prime_UTR 2229457 2229674 2229457 ID=Msa0710240-mRNA-1:three_prime_utr;Parent=Msa0710240-mRNA-1
Gene Sequence

>Msa0710240

ATGAGGGGAGAAGATCTTGAAGGTTTGAATGTGGAAGAATTACAACAATTGGAGAGGTCACTTGAAATTGGGTTGGGCCGTGTAATAGAAAACAAGGGAGAGAAGATCATGATGGAGATCAATGATCTCCAAAGAAAGGGAAGGCAACTGATGGAAGAGAACGACCGACTAAAGCAACATGTTGCGGGCATGATTAACGACAGAATGGTTGGAGGTGATGAATATGAGAATGTGGTTAATGAAGGTCAATCCTCAGAATCTGTCACCAACGTTTACAACTCCATTGGTCCTCCCCAAGATTATGAGAGTTCAGATACATCGCTCAAGTTGGGGCTCCCATATACCGGCTGA
Protein sequence

>Msa0710240

MRGEDLEGLNVEELQQLERSLEIGLGRVIENKGEKIMMEINDLQRKGRQLMEENDRLKQHVAGMINDRMVGGDEYENVVNEGQSSESVTNVYNSIGPPQDYESSDTSLKLGLPYTG*