AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0710900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0710900 MtrunA17_Chr5g0400671 93.293 328 20 1 18 345 1 326 0.0 605
Msa0710900 MtrunA17_Chr4g0054561 46.110 347 141 13 18 341 1 324 1.06e-80 249
Msa0710900 MtrunA17_Chr4g0054551 43.228 347 151 13 18 341 1 324 5.01e-75 234
Msa0710900 MtrunA17_Chr2g0280161 35.795 352 188 12 19 340 5 348 1.36e-52 177
Msa0710900 MtrunA17_Chr4g0049331 33.540 322 186 10 32 341 1 306 7.94e-48 163
Msa0710900 MtrunA17_Chr5g0400661 39.916 238 122 6 121 342 102 334 1.02e-45 159
Msa0710900 MtrunA17_Chr5g0400621 38.023 263 143 4 80 340 83 327 7.98e-45 156
Msa0710900 MtrunA17_Chr4g0002781 43.750 192 98 3 158 342 161 349 2.54e-43 153
Msa0710900 MtrunA17_Chr4g0002801 39.823 226 117 4 133 342 108 330 3.32e-42 149
Msa0710900 MtrunA17_Chr2g0333621 36.047 258 139 6 92 342 73 311 3.94e-42 149
Msa0710900 MtrunA17_Chr2g0333611 40.000 185 102 3 162 342 36 215 2.21e-41 144
Msa0710900 MtrunA17_Chr4g0033391 41.436 181 100 2 164 342 140 316 7.11e-41 145
Msa0710900 MtrunA17_Chr8g0336681 39.153 189 103 3 164 342 149 335 1.20e-39 143
Msa0710900 MtrunA17_Chr7g0225901 45.161 155 84 1 168 321 85 239 2.60e-39 139
Msa0710900 MtrunA17_Chr2g0307131 41.489 188 104 2 158 339 161 348 6.88e-39 141
Msa0710900 MtrunA17_Chr4g0033291 41.262 206 104 4 143 342 130 324 2.56e-38 139
Msa0710900 MtrunA17_Chr2g0307141 41.860 172 99 1 169 339 181 352 4.51e-38 139
Msa0710900 MtrunA17_Chr6g0468491 41.379 174 101 1 166 338 132 305 5.44e-38 137
Msa0710900 MtrunA17_Chr4g0054581 40.201 199 103 6 155 342 181 374 1.64e-37 138
Msa0710900 MtrunA17_Chr7g0249811 39.665 179 100 1 164 342 158 328 1.15e-36 135
Msa0710900 MtrunA17_Chr5g0400641 44.079 152 84 1 174 324 1 152 2.16e-36 129
Msa0710900 MtrunA17_Chr2g0333641 34.263 251 139 6 104 341 69 306 8.14e-36 132
Msa0710900 MtrunA17_Chr4g0033281 38.308 201 116 4 143 342 128 321 5.05e-35 130
Msa0710900 MtrunA17_Chr2g0333541 40.860 186 103 3 164 344 87 270 1.37e-32 122
Msa0710900 MtrunA17_Chr4g0054621 35.263 190 114 3 155 342 155 337 2.30e-32 123
Msa0710900 MtrunA17_Chr4g0054571 32.867 286 148 8 80 342 65 329 1.81e-31 120
Msa0710900 MtrunA17_Chr6g0468461 39.744 156 81 1 166 321 168 310 1.12e-29 116
Msa0710900 MtrunA17_Chr4g0002851 35.762 151 80 1 194 344 65 198 1.44e-20 88.2
Msa0710900 MtrunA17_Chr8g0366751 35.583 163 71 4 164 301 451 604 4.62e-20 91.3
Msa0710900 MtrunA17_Chr7g0233441 33.775 151 91 1 194 344 57 198 3.18e-19 84.7
Msa0710900 MtrunA17_Chr4g0002831 31.098 164 102 3 182 344 53 206 1.69e-18 82.8
Msa0710900 MtrunA17_Chr1g0185621 33.333 183 104 5 172 341 476 653 4.00e-18 85.5
Msa0710900 MtrunA17_Chr5g0411341 35.758 165 76 5 164 305 482 639 8.77e-18 84.7
Msa0710900 MtrunA17_Chr7g0275821 28.729 181 106 2 172 344 253 418 1.03e-17 83.6
Msa0710900 MtrunA17_Chr7g0252041 30.481 187 110 4 171 343 286 466 7.59e-16 78.2
Msa0710900 MtrunA17_Chr8g0346151 36.029 136 71 4 172 295 280 411 2.29e-15 77.0
Msa0710900 MtrunA17_Chr8g0346161 29.480 173 117 2 172 339 292 464 1.08e-14 74.7
Msa0710900 MtrunA17_Chr8g0365711 25.146 171 122 2 155 319 228 398 1.55e-14 74.3
Msa0710900 MtrunA17_Chr3g0131691 30.818 159 95 5 171 316 298 454 2.01e-13 70.9
Msa0710900 MtrunA17_Chr1g0211081 32.836 134 81 3 103 235 256 381 4.74e-13 70.1
Msa0710900 MtrunA17_Chr7g0260841 34.146 123 54 3 162 280 342 441 5.28e-13 69.7
Msa0710900 MtrunA17_Chr5g0411541 26.596 282 170 9 85 342 47 315 4.84e-12 66.2
Msa0710900 MtrunA17_Chr4g0028071 30.496 141 83 4 173 304 151 285 9.89e-12 65.1
Msa0710900 MtrunA17_Chr8g0386921 27.711 166 84 4 172 304 407 569 1.66e-11 65.5
Msa0710900 MtrunA17_Chr2g0320291 30.714 140 84 4 173 306 135 267 2.39e-11 63.9
Msa0710900 MtrunA17_Chr2g0298751 24.352 193 130 5 149 326 29 220 3.99e-11 62.4
Msa0710900 MtrunA17_Chr3g0141091 29.379 177 106 4 167 342 169 327 4.55e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0710900 AT4G37850.2 35.988 339 186 13 19 341 1 324 3.71e-49 168
Msa0710900 AT4G37850.1 35.988 339 186 13 19 341 1 324 3.71e-49 168
Msa0710900 AT2G22760.1 43.784 185 99 2 163 342 111 295 1.34e-45 157
Msa0710900 AT2G22760.2 46.012 163 84 1 163 321 111 273 4.06e-43 150
Msa0710900 AT2G22750.3 33.819 343 153 14 19 323 5 311 3.43e-42 150
Msa0710900 AT2G22750.5 33.819 343 153 14 19 323 1 307 3.66e-42 149
Msa0710900 AT2G22750.4 33.819 343 153 14 19 323 5 311 1.16e-41 148
Msa0710900 AT2G22750.2 45.763 177 69 4 163 323 118 283 2.74e-39 141
Msa0710900 AT2G22750.1 45.763 177 69 4 163 323 118 283 9.14e-39 140
Msa0710900 AT2G22770.1 39.810 211 109 3 149 341 108 318 7.07e-37 135
Msa0710900 AT4G17880.1 30.556 216 116 6 108 306 354 552 4.90e-20 91.7
Msa0710900 AT5G46760.1 34.783 161 78 4 164 306 404 555 5.99e-19 88.2
Msa0710900 AT1G32640.1 35.099 151 92 3 162 306 439 589 1.28e-16 81.3
Msa0710900 AT2G46510.1 27.322 183 106 3 162 337 382 544 4.02e-16 79.7
Msa0710900 AT1G01260.1 35.099 151 64 4 167 307 429 555 5.20e-16 79.3
Msa0710900 AT1G01260.2 35.099 151 64 4 167 307 429 555 5.20e-16 79.3
Msa0710900 AT1G01260.3 35.099 151 64 4 167 307 429 555 5.20e-16 79.3
Msa0710900 AT5G57150.4 26.455 189 119 3 149 322 33 216 2.96e-15 75.1
Msa0710900 AT5G57150.5 26.344 186 117 3 149 319 32 212 3.59e-15 74.3
Msa0710900 AT5G57150.2 26.344 186 117 3 149 319 33 213 4.76e-15 73.9
Msa0710900 AT5G57150.3 26.344 186 117 3 149 319 33 213 5.35e-15 73.6
Msa0710900 AT5G57150.1 26.154 195 124 3 149 328 32 221 5.69e-15 73.9
Msa0710900 AT1G63650.3 29.381 194 103 6 172 341 406 589 1.11e-14 75.5
Msa0710900 AT1G63650.1 29.381 194 103 6 172 341 406 589 1.11e-14 75.5
Msa0710900 AT1G63650.2 29.381 194 103 6 172 341 406 589 1.11e-14 75.5
Msa0710900 AT1G12860.1 29.444 180 115 4 171 343 267 441 1.93e-14 74.3
Msa0710900 AT4G09820.1 34.884 129 63 2 172 299 364 472 1.96e-14 74.7
Msa0710900 AT1G12860.2 31.373 153 98 3 171 316 267 419 2.42e-14 73.9
Msa0710900 AT2G16910.1 27.684 177 105 4 172 325 315 491 1.55e-13 72.0
Msa0710900 AT4G29930.5 30.345 145 88 3 172 313 55 189 1.78e-13 69.7
Msa0710900 AT4G29930.2 30.345 145 88 3 172 313 55 189 2.78e-13 68.9
Msa0710900 AT4G29930.6 30.822 146 89 3 172 313 55 192 3.23e-13 68.6
Msa0710900 AT5G41315.2 29.146 199 102 9 172 341 450 638 2.57e-12 68.2
Msa0710900 AT5G41315.3 29.146 199 102 9 172 341 447 635 2.73e-12 68.2
Msa0710900 AT5G41315.1 29.146 199 102 9 172 341 442 630 3.12e-12 68.2
Msa0710900 AT5G65640.1 28.889 180 112 5 167 342 174 341 4.13e-12 67.0
Msa0710900 AT4G16430.1 45.333 75 37 1 162 232 307 381 6.61e-12 66.6
Msa0710900 AT3G26744.1 29.545 176 106 6 171 334 307 476 7.78e-12 66.6
Msa0710900 AT3G26744.4 29.545 176 106 6 171 334 307 476 7.78e-12 66.6
Msa0710900 AT3G26744.2 29.545 176 106 6 171 334 307 476 7.78e-12 66.6
Msa0710900 AT3G26744.6 31.013 158 96 5 171 316 307 463 1.01e-11 66.2
Msa0710900 AT4G00870.1 29.927 137 92 1 172 304 250 386 1.31e-11 65.9
Msa0710900 AT4G29930.3 26.667 165 99 3 171 313 54 218 2.35e-11 63.9
Msa0710900 AT4G29930.7 26.667 165 99 3 171 313 54 218 2.35e-11 63.9
Msa0710900 AT4G29930.1 26.829 164 98 3 172 313 55 218 3.15e-11 63.2

Find 91 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTCAACTGGTTAATTT+TGG 0.110602 5_1:+2788064 None:intergenic
TTTCTCATCTTACACCAATT+TGG 0.189336 5_1:-2790721 None:intergenic
GATACTCTTACTTCGATTTC+AGG 0.236620 5_1:+2788891 None:intergenic
GTTTCAACTTGGAAACAATT+TGG 0.254789 5_1:+2790069 None:intergenic
TACTTTGGACAGTGAGATTA+AGG 0.260165 5_1:+2788790 None:intergenic
TTCAGGAAGTGGTTGGTTAT+TGG 0.276191 5_1:+2788908 None:intergenic
TCCTGTTGTGAAAAGGTTCT+AGG 0.284232 5_1:-2787628 Msa0710900:three_prime_UTR
GCTCTTTCAGCCATTGTTCC+TGG 0.288584 5_1:-2789700 Msa0710900:CDS
CCAAAGTTGTCGAATAATAT+TGG 0.304765 5_1:+2790120 None:intergenic
TGTCCTTTGATAAGGATTCT+AGG 0.308395 5_1:-2787963 Msa0710900:three_prime_UTR
TCATCATCATCGTCATCATT+AGG 0.314724 5_1:+2788942 None:intergenic
TCGAGCTTGAGCAGTGTTCC+TGG 0.329696 5_1:+2789777 None:intergenic
AGAGAGTTAATATTCCAAAT+TGG 0.339623 5_1:+2790707 None:intergenic
CCTTCTTTCCTGTTGTGAAA+AGG 0.358968 5_1:-2787635 Msa0710900:three_prime_UTR
ACTAGATATCAAAACTATAA+TGG 0.366097 5_1:+2787524 None:intergenic
GATTAAAATATTCCTTCAAC+TGG 0.373209 5_1:+2788054 None:intergenic
CATCACAGGACCTTCCTTCA+TGG 0.375414 5_1:+2787930 None:intergenic
CGATTTGATTAGATGTGGTA+TGG 0.375743 5_1:+2790477 None:intergenic
AATGAATTGAGGTGATGAAT+AGG 0.381227 5_1:+2789987 None:intergenic
CATTGTTCCTGGCCTCAAAA+AGG 0.384959 5_1:-2789689 Msa0710900:intron
CTTCCAGCTTTGGTTTCTCA+AGG 0.393979 5_1:-2789853 Msa0710900:CDS
TAAAATTGCTTAGAAGCAAT+TGG 0.399867 5_1:+2789940 None:intergenic
GTTGTTTCCAAAGGGCAAAA+AGG 0.400410 5_1:+2788764 None:intergenic
CATCATCATCGTCATCATTA+GGG 0.403065 5_1:+2788943 None:intergenic
CCAATATTATTCGACAACTT+TGG 0.407380 5_1:-2790120 Msa0710900:CDS
AGTAGTATGCTTACAAGTTC+AGG 0.414356 5_1:+2790401 None:intergenic
AGAAGCAATTGGTGTTGAAC+TGG 0.425456 5_1:+2789951 None:intergenic
ATGGACAAAGCTTCTGTGTT+GGG 0.439391 5_1:-2789080 Msa0710900:CDS
AAATAAATTAGAAGAGAAAG+TGG 0.442399 5_1:+2790677 None:intergenic
TGTGACAGCAAAGGATCTCC+TGG 0.446189 5_1:-2788112 Msa0710900:CDS
CAAGAGAATCCATCGGGTAT+TGG 0.448559 5_1:+2790148 None:intergenic
GTCCTTTGATAAGGATTCTA+GGG 0.456194 5_1:-2787962 Msa0710900:three_prime_UTR
TGAAAGAGCGATGAAACTTC+GGG 0.456547 5_1:+2789714 None:intergenic
CATCAAGATGAGTTTCAACT+TGG 0.457168 5_1:+2790058 None:intergenic
ATCCAAAATTAACCAGTTGA+AGG 0.460869 5_1:-2788066 Msa0710900:exon
GGACGTGCCGCTACGGTGCT+TGG 0.467945 5_1:-2788825 Msa0710900:CDS
ATCAACAATGTTGTTTCCAA+AGG 0.469768 5_1:+2788755 None:intergenic
TCAACAATGTTGTTTCCAAA+GGG 0.475298 5_1:+2788756 None:intergenic
CTGAAAGAGCGATGAAACTT+CGG 0.488970 5_1:+2789713 None:intergenic
TGAGGTGATGAATAGGAAGA+AGG 0.490585 5_1:+2789994 None:intergenic
AAGCCTGTCGTATGCTTCCC+AGG 0.497417 5_1:+2788094 None:intergenic
TTCTACAACATGTTCTGGGT+CGG 0.501517 5_1:-2787835 Msa0710900:three_prime_UTR
ATTAACAATTACACATGTGA+TGG 0.502857 5_1:+2787724 None:intergenic
CTTCGATTTCAGGAAGTGGT+TGG 0.508259 5_1:+2788901 None:intergenic
TAAAACCTAAAGTCGAGAAA+AGG 0.508413 5_1:-2789897 Msa0710900:CDS
TGTTCCTGGTCTGATTCACT+TGG 0.510038 5_1:+2789791 None:intergenic
GAGGTGATGAATAGGAAGAA+GGG 0.522927 5_1:+2789995 None:intergenic
CTTACTTCGATTTCAGGAAG+TGG 0.529255 5_1:+2788897 None:intergenic
TTTATTTACCTTAGCAACAA+TGG 0.529541 5_1:+2788728 None:intergenic
GATTTGATTAGATGTGGTAT+GGG 0.531705 5_1:+2790478 None:intergenic
GAACCTTGAGAAACCAAAGC+TGG 0.540922 5_1:+2789850 None:intergenic
TGATGTAACCATTGTTGCTA+AGG 0.545162 5_1:-2788736 Msa0710900:intron
ACAAGTTCAGGCACATAGGT+GGG 0.548457 5_1:+2790413 None:intergenic
ATCATAGATTCTCCCATCAC+AGG 0.549532 5_1:+2787916 None:intergenic
ACAAGCACGTTTGCAGACAC+TGG 0.553923 5_1:-2789030 Msa0710900:CDS
TCTAGGTTTGATAGAAAATG+TGG 0.554779 5_1:-2787611 Msa0710900:three_prime_UTR
AATAATCACAAAACGACAAG+AGG 0.560394 5_1:+2787562 None:intergenic
AACTTCGATTTGATTAGATG+TGG 0.560569 5_1:+2790472 None:intergenic
ATGAAGGAAGGTCCTGTGAT+GGG 0.560852 5_1:-2787928 Msa0710900:three_prime_UTR
ACACAGTGGACGTGCCGCTA+CGG 0.562208 5_1:-2788832 Msa0710900:CDS
AATTCTCTTGCAGAGGAACA+AGG 0.562972 5_1:-2788142 Msa0710900:intron
AACCCTAGAATCCTTATCAA+AGG 0.573151 5_1:+2787960 None:intergenic
CAAGTTCAGGCACATAGGTG+GGG 0.574321 5_1:+2790414 None:intergenic
CTTCTTTAACCAATACCCGA+TGG 0.574424 5_1:-2790157 Msa0710900:CDS
TTTGCATAGTTGACTTGACT+AGG 0.577273 5_1:-2787762 Msa0710900:three_prime_UTR
TGTTCAAAAGAAATGAATTG+AGG 0.577900 5_1:+2789976 None:intergenic
ACATGTTCTGGGTCGGTGTG+TGG 0.580330 5_1:-2787828 Msa0710900:three_prime_UTR
CATGAAGGAAGGTCCTGTGA+TGG 0.581540 5_1:-2787929 Msa0710900:three_prime_UTR
CAGGAAAGAAGGAAGAAAAG+AGG 0.581719 5_1:+2787646 None:intergenic
TAGGGTTACTATATCCATGA+AGG 0.583367 5_1:-2787944 Msa0710900:three_prime_UTR
TCAAGCTCGAGAACATGTAA+TGG 0.585662 5_1:-2789764 Msa0710900:CDS
TACAAGTTCAGGCACATAGG+TGG 0.586428 5_1:+2790412 None:intergenic
GAGATGCAAGAGAATCCATC+GGG 0.590902 5_1:+2790142 None:intergenic
GCTTACAAGTTCAGGCACAT+AGG 0.592149 5_1:+2790409 None:intergenic
AACTTGGAAACAATTTGGTG+TGG 0.596690 5_1:+2790074 None:intergenic
CTCATCTTGATGATCAAACA+AGG 0.597867 5_1:-2790047 Msa0710900:CDS
GTGTGTGGAATTTCAACAGA+TGG 0.598947 5_1:-2787813 Msa0710900:three_prime_UTR
TCTCCTGGGAAGCATACGAC+AGG 0.600421 5_1:-2788097 Msa0710900:CDS
GATGGACAAAGCTTCTGTGT+TGG 0.607222 5_1:-2789081 Msa0710900:CDS
GTGACAGCAAAGGATCTCCT+GGG 0.607386 5_1:-2788111 Msa0710900:CDS
AGATCTCTTCACTTGAACTG+TGG 0.609751 5_1:+2788974 None:intergenic
GTTACTATATCCATGAAGGA+AGG 0.623815 5_1:-2787940 Msa0710900:three_prime_UTR
GGAAAACTGTGTGACAGCAA+AGG 0.624039 5_1:-2788121 Msa0710900:CDS
GGAGATGCAAGAGAATCCAT+CGG 0.627162 5_1:+2790141 None:intergenic
AAGTTCAGGCACATAGGTGG+GGG 0.650925 5_1:+2790415 None:intergenic
AATGGCCGAAAGAAAACGAA+GGG 0.657033 5_1:-2789746 Msa0710900:CDS
TAATTGGCCAAGCACCGTAG+CGG 0.678187 5_1:+2788818 None:intergenic
AGCACGTTTGCAGACACTGG+AGG 0.686426 5_1:-2789027 Msa0710900:CDS
ATAACCAAGTGAATCAGACC+AGG 0.687464 5_1:-2789795 Msa0710900:CDS
TAATGGCCGAAAGAAAACGA+AGG 0.695210 5_1:-2789747 Msa0710900:CDS
ATTCAATGTGACAAACACAG+TGG 0.762327 5_1:-2788846 Msa0710900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATAATTTATTTTTCTTT+TGG - chr5_1:2788741-2788760 Msa0710900:CDS 10.0%
!! AATTAATGTATTCAATATTA+TGG - chr5_1:2789120-2789139 Msa0710900:intron 10.0%
!! AAAATAAATTAACATTTCTA+AGG - chr5_1:2789588-2789607 Msa0710900:intron 10.0%
!!! AAAAAATCATAAATTTTAGA+CGG - chr5_1:2789831-2789850 Msa0710900:CDS 10.0%
!!! TAATTTTTCTAATCATTATT+TGG - chr5_1:2790527-2790546 Msa0710900:intron 10.0%
!!! AAGTTTATGAATTTTAAGAT+AGG + chr5_1:2787864-2787883 None:intergenic 15.0%
!!! TTGTTTTTATTTCATTTTCT+AGG - chr5_1:2788906-2788925 Msa0710900:CDS 15.0%
!! TACAATATAAATATTGCAAT+TGG + chr5_1:2789083-2789102 None:intergenic 15.0%
!! TATAGGAAAATATTACATAT+AGG - chr5_1:2789551-2789570 Msa0710900:intron 15.0%
!!! AAATTTTGAAATGAAAAATG+TGG - chr5_1:2789854-2789873 Msa0710900:CDS 15.0%
!! TTTATGAATTATGATAATCT+AGG + chr5_1:2790066-2790085 None:intergenic 15.0%
!!! TTATGATTCATTTATTTTCA+AGG - chr5_1:2790365-2790384 Msa0710900:intron 15.0%
!!! TATGATTCATTTATTTTCAA+GGG - chr5_1:2790366-2790385 Msa0710900:intron 15.0%
!! TACAATATTAAAAAAAGAAG+TGG + chr5_1:2790650-2790669 None:intergenic 15.0%
!! AAATAAATTAGAAGAGAAAG+TGG + chr5_1:2787558-2787577 None:intergenic 20.0%
!!! TTTATTTTCTTCAATCTTCA+AGG - chr5_1:2787571-2787590 Msa0710900:three_prime_UTR 20.0%
!! AAGCAGATTAAGAATTAAAT+AGG + chr5_1:2787600-2787619 None:intergenic 20.0%
!!! TTAATCTGCTTTTTCTTTTA+GGG - chr5_1:2787609-2787628 Msa0710900:three_prime_UTR 20.0%
!!! TCTTTAATTACTTTTGAACA+GGG - chr5_1:2788037-2788056 Msa0710900:three_prime_UTR 20.0%
!! ACATTAATGATCCATATTAA+AGG - chr5_1:2788651-2788670 Msa0710900:intron 20.0%
!!! TATTTTCTTATGATCAGATT+AGG + chr5_1:2788806-2788825 None:intergenic 20.0%
!! GCATACTTGAAAATAATAAA+CGG - chr5_1:2789749-2789768 Msa0710900:CDS 20.0%
!! CCACAAAAAAAAAAAAAAAG+AGG + chr5_1:2789882-2789901 None:intergenic 20.0%
!!! CCTCTTTTTTTTTTTTTTTG+TGG - chr5_1:2789879-2789898 Msa0710900:CDS 20.0%
!!! CTTTTTTTTTTTTTTTGTGG+TGG - chr5_1:2789882-2789901 Msa0710900:CDS 20.0%
!!! TTTTCTAATCATTATTTGGA+TGG - chr5_1:2790531-2790550 Msa0710900:intron 20.0%
!! ACTAGATATCAAAACTATAA+TGG + chr5_1:2790711-2790730 None:intergenic 20.0%
! AGAGAGTTAATATTCCAAAT+TGG + chr5_1:2787528-2787547 None:intergenic 25.0%
!!! CTTAATCTGCTTTTTCTTTT+AGG - chr5_1:2787608-2787627 Msa0710900:three_prime_UTR 25.0%
! GTTGAAATCTTAGAATTGAA+GGG + chr5_1:2787691-2787710 None:intergenic 25.0%
! AGTTGAAATCTTAGAATTGA+AGG + chr5_1:2787692-2787711 None:intergenic 25.0%
!! TCTAATCAAATCGAAGTTTT+AGG - chr5_1:2787765-2787784 Msa0710900:three_prime_UTR 25.0%
!!! CTCTTTAATTACTTTTGAAC+AGG - chr5_1:2788036-2788055 Msa0710900:three_prime_UTR 25.0%
! TGTTCAAAAGAAATGAATTG+AGG + chr5_1:2788259-2788278 None:intergenic 25.0%
! TAAAATTGCTTAGAAGCAAT+TGG + chr5_1:2788295-2788314 None:intergenic 25.0%
! ATTATAACATGTTTGTGAGT+TGG + chr5_1:2788582-2788601 None:intergenic 25.0%
! TGATATAGAAATGGTTTGAA+TGG + chr5_1:2788611-2788630 None:intergenic 25.0%
! GTAGTATGTTGATATAGAAA+TGG + chr5_1:2788620-2788639 None:intergenic 25.0%
! TATTGACGAAAAACATGAAA+TGG + chr5_1:2788727-2788746 None:intergenic 25.0%
! TTGGTAAGTTCAACTTAATT+AGG + chr5_1:2789064-2789083 None:intergenic 25.0%
!! TGAGATTAAGGTTTTCTAAT+TGG + chr5_1:2789433-2789452 None:intergenic 25.0%
! TTTATTTACCTTAGCAACAA+TGG + chr5_1:2789507-2789526 None:intergenic 25.0%
!!! TAACATTTCTAAGGTTGTTT+AGG - chr5_1:2789597-2789616 Msa0710900:intron 25.0%
! AAATATTAATTCTCTTGCAG+AGG - chr5_1:2790083-2790102 Msa0710900:CDS 25.0%
! GATTAAAATATTCCTTCAAC+TGG + chr5_1:2790181-2790200 None:intergenic 25.0%
! ATTAACAATTACACATGTGA+TGG + chr5_1:2790511-2790530 None:intergenic 25.0%
!!! TTTTTTGTCTTCGAACATGT+TGG - chr5_1:2787648-2787667 Msa0710900:three_prime_UTR 30.0%
GATTTGATTAGATGTGGTAT+GGG + chr5_1:2787757-2787776 None:intergenic 30.0%
! AACTTCGATTTGATTAGATG+TGG + chr5_1:2787763-2787782 None:intergenic 30.0%
!!! TTGACCAAGCTTTATTTTTC+TGG - chr5_1:2787958-2787977 Msa0710900:three_prime_UTR 30.0%
!! TTATTTTTCTGGTCGAACTT+TGG - chr5_1:2787969-2787988 Msa0710900:three_prime_UTR 30.0%
!! AATTACTTTTGAACAGGGAA+TGG - chr5_1:2788042-2788061 Msa0710900:three_prime_UTR 30.0%
CCAAAGTTGTCGAATAATAT+TGG + chr5_1:2788115-2788134 None:intergenic 30.0%
CCAATATTATTCGACAACTT+TGG - chr5_1:2788112-2788131 Msa0710900:CDS 30.0%
GTTTCAACTTGGAAACAATT+TGG + chr5_1:2788166-2788185 None:intergenic 30.0%
!!! TATCGATGTTTTGATCTTCT+TGG + chr5_1:2788215-2788234 None:intergenic 30.0%
AATGAATTGAGGTGATGAAT+AGG + chr5_1:2788248-2788267 None:intergenic 30.0%
! GTTTTACATCGGAATAATCA+AGG + chr5_1:2788321-2788340 None:intergenic 30.0%
TAAAACCTAAAGTCGAGAAA+AGG - chr5_1:2788335-2788354 Msa0710900:intron 30.0%
! GATAAGATCATACCTTTTTG+AGG + chr5_1:2788558-2788577 None:intergenic 30.0%
ATCCATATTAAAGGCTGAAA+TGG - chr5_1:2788660-2788679 Msa0710900:intron 30.0%
AATGGTCACTAATATTGCTA+GGG + chr5_1:2788709-2788728 None:intergenic 30.0%
AAATGGTCACTAATATTGCT+AGG + chr5_1:2788710-2788729 None:intergenic 30.0%
AAGAGTAGACATCAAAAAGT+TGG - chr5_1:2788845-2788864 Msa0710900:CDS 30.0%
AGAGTAGACATCAAAAAGTT+GGG - chr5_1:2788846-2788865 Msa0710900:CDS 30.0%
!!! TCATTTTCTAGGATCAAATC+AGG - chr5_1:2788917-2788936 Msa0710900:CDS 30.0%
AATATTATGGTATGTGCAGA+TGG - chr5_1:2789133-2789152 Msa0710900:intron 30.0%
!! TCAACAATGTTGTTTCCAAA+GGG + chr5_1:2789479-2789498 None:intergenic 30.0%
!! ATCAACAATGTTGTTTCCAA+AGG + chr5_1:2789480-2789499 None:intergenic 30.0%
AAAACCAGACAATTATGACA+TGG - chr5_1:2789631-2789650 Msa0710900:intron 30.0%
ATCCAAAATTAACCAGTTGA+AGG - chr5_1:2790166-2790185 Msa0710900:CDS 30.0%
! TTCCTTCAACTGGTTAATTT+TGG + chr5_1:2790171-2790190 None:intergenic 30.0%
!!! TCCTTTTTTGTCCTTTGATA+AGG - chr5_1:2790261-2790280 Msa0710900:intron 30.0%
TCCTTATCAAAGGACAAAAA+AGG + chr5_1:2790265-2790284 None:intergenic 30.0%
! TCTTTTCTACAACATGTTCT+GGG - chr5_1:2790393-2790412 Msa0710900:intron 30.0%
ACAACAGAAGAAAAACACTT+TGG + chr5_1:2790447-2790466 None:intergenic 30.0%
TCTAGGTTTGATAGAAAATG+TGG - chr5_1:2790621-2790640 Msa0710900:intron 30.0%
AATAATCACAAAACGACAAG+AGG + chr5_1:2790673-2790692 None:intergenic 30.0%
!!! TCGAACATGTTGGTTTGTTT+TGG - chr5_1:2787658-2787677 Msa0710900:three_prime_UTR 35.0%
CGATTTGATTAGATGTGGTA+TGG + chr5_1:2787758-2787777 None:intergenic 35.0%
AGTAGTATGCTTACAAGTTC+AGG + chr5_1:2787834-2787853 None:intergenic 35.0%
TCGACCAGAAAAATAAAGCT+TGG + chr5_1:2787965-2787984 None:intergenic 35.0%
! AACTTGGAAACAATTTGGTG+TGG + chr5_1:2788161-2788180 None:intergenic 35.0%
CATCAAGATGAGTTTCAACT+TGG + chr5_1:2788177-2788196 None:intergenic 35.0%
! CTCATCTTGATGATCAAACA+AGG - chr5_1:2788185-2788204 Msa0710900:intron 35.0%
!!! GATGTTTTGATCTTCTTGGT+TGG + chr5_1:2788211-2788230 None:intergenic 35.0%
!! CTCGACTTTAGGTTTTACAT+CGG + chr5_1:2788332-2788351 None:intergenic 35.0%
! TTACACCTTTTCTCGACTTT+AGG + chr5_1:2788343-2788362 None:intergenic 35.0%
! CAAAGATTTTCTTCCAGCTT+TGG - chr5_1:2788369-2788388 Msa0710900:intron 35.0%
! CTTTTCCCTTCGTTTTCTTT+CGG + chr5_1:2788494-2788513 None:intergenic 35.0%
CACCATTTCAGCCTTTAATA+TGG + chr5_1:2788665-2788684 None:intergenic 35.0%
GAGTAGACATCAAAAAGTTG+GGG - chr5_1:2788847-2788866 Msa0710900:CDS 35.0%
AGTAATGTTCTGCTTACACT+AGG - chr5_1:2788977-2788996 Msa0710900:CDS 35.0%
GCTGAAGACAACAAAAAAAC+TGG - chr5_1:2789233-2789252 Msa0710900:intron 35.0%
CATCATCATCGTCATCATTA+GGG + chr5_1:2789292-2789311 None:intergenic 35.0%
TCATCATCATCGTCATCATT+AGG + chr5_1:2789293-2789312 None:intergenic 35.0%
GATACTCTTACTTCGATTTC+AGG + chr5_1:2789344-2789363 None:intergenic 35.0%
ATTCAATGTGACAAACACAG+TGG - chr5_1:2789386-2789405 Msa0710900:intron 35.0%
TACTTTGGACAGTGAGATTA+AGG + chr5_1:2789445-2789464 None:intergenic 35.0%
TGATGTAACCATTGTTGCTA+AGG - chr5_1:2789496-2789515 Msa0710900:intron 35.0%
TCAGCAACGAAAATTAGTGA+CGG + chr5_1:2789982-2790001 None:intergenic 35.0%
TGTCCTTTGATAAGGATTCT+AGG - chr5_1:2790269-2790288 Msa0710900:intron 35.0%
GTCCTTTGATAAGGATTCTA+GGG - chr5_1:2790270-2790289 Msa0710900:intron 35.0%
AACCCTAGAATCCTTATCAA+AGG + chr5_1:2790275-2790294 None:intergenic 35.0%
TAGGGTTACTATATCCATGA+AGG - chr5_1:2790288-2790307 Msa0710900:intron 35.0%
GTTACTATATCCATGAAGGA+AGG - chr5_1:2790292-2790311 Msa0710900:intron 35.0%
! GTCTTTTCTACAACATGTTC+TGG - chr5_1:2790392-2790411 Msa0710900:intron 35.0%
!! TTTGCATAGTTGACTTGACT+AGG - chr5_1:2790470-2790489 Msa0710900:CDS 35.0%
! TACTTTTGAACAGGGAATGG+AGG - chr5_1:2788045-2788064 Msa0710900:three_prime_UTR 40.0%
CTTCTTTAACCAATACCCGA+TGG - chr5_1:2788075-2788094 Msa0710900:exon 40.0%
GAGGTGATGAATAGGAAGAA+GGG + chr5_1:2788240-2788259 None:intergenic 40.0%
TGAGGTGATGAATAGGAAGA+AGG + chr5_1:2788241-2788260 None:intergenic 40.0%
!! AGAAGCAATTGGTGTTGAAC+TGG + chr5_1:2788284-2788303 None:intergenic 40.0%
ATAACCAAGTGAATCAGACC+AGG - chr5_1:2788437-2788456 Msa0710900:intron 40.0%
TCAAGCTCGAGAACATGTAA+TGG - chr5_1:2788468-2788487 Msa0710900:intron 40.0%
TAATGGCCGAAAGAAAACGA+AGG - chr5_1:2788485-2788504 Msa0710900:intron 40.0%
AATGGCCGAAAGAAAACGAA+GGG - chr5_1:2788486-2788505 Msa0710900:intron 40.0%
TGAAAGAGCGATGAAACTTC+GGG + chr5_1:2788521-2788540 None:intergenic 40.0%
CTGAAAGAGCGATGAAACTT+CGG + chr5_1:2788522-2788541 None:intergenic 40.0%
ATGGACAAAGCTTCTGTGTT+GGG - chr5_1:2789152-2789171 Msa0710900:intron 40.0%
AGATCTCTTCACTTGAACTG+TGG + chr5_1:2789261-2789280 None:intergenic 40.0%
! TTCAGGAAGTGGTTGGTTAT+TGG + chr5_1:2789327-2789346 None:intergenic 40.0%
CTTACTTCGATTTCAGGAAG+TGG + chr5_1:2789338-2789357 None:intergenic 40.0%
!! AGGGCAAAAAGGTACTACTT+TGG + chr5_1:2789460-2789479 None:intergenic 40.0%
! AGTAGTACCTTTTTGCCCTT+TGG - chr5_1:2789461-2789480 Msa0710900:intron 40.0%
! GTTGTTTCCAAAGGGCAAAA+AGG + chr5_1:2789471-2789490 None:intergenic 40.0%
ACGACCATGTCATAATTGTC+TGG + chr5_1:2789638-2789657 None:intergenic 40.0%
!!! TTTTTTTTTTGTGGTGGCCG+AGG - chr5_1:2789888-2789907 Msa0710900:CDS 40.0%
TTAGTGAGATGTTCCAACCT+CGG + chr5_1:2789908-2789927 None:intergenic 40.0%
CAGCAACGAAAATTAGTGAC+GGG + chr5_1:2789981-2790000 None:intergenic 40.0%
AATTCTCTTGCAGAGGAACA+AGG - chr5_1:2790090-2790109 Msa0710900:CDS 40.0%
ATCATAGATTCTCCCATCAC+AGG + chr5_1:2790319-2790338 None:intergenic 40.0%
! TTCTACAACATGTTCTGGGT+CGG - chr5_1:2790397-2790416 Msa0710900:intron 40.0%
GTGTGTGGAATTTCAACAGA+TGG - chr5_1:2790419-2790438 Msa0710900:CDS 40.0%
CAGGAAAGAAGGAAGAAAAG+AGG + chr5_1:2790589-2790608 None:intergenic 40.0%
! CCTTTTCACAACAGGAAAGA+AGG + chr5_1:2790600-2790619 None:intergenic 40.0%
CCTTCTTTCCTGTTGTGAAA+AGG - chr5_1:2790597-2790616 Msa0710900:intron 40.0%
TCCTGTTGTGAAAAGGTTCT+AGG - chr5_1:2790604-2790623 Msa0710900:intron 40.0%
! ACCTAGAACCTTTTCACAAC+AGG + chr5_1:2790608-2790627 None:intergenic 40.0%
ACAAGTTCAGGCACATAGGT+GGG + chr5_1:2787822-2787841 None:intergenic 45.0%
TACAAGTTCAGGCACATAGG+TGG + chr5_1:2787823-2787842 None:intergenic 45.0%
GCTTACAAGTTCAGGCACAT+AGG + chr5_1:2787826-2787845 None:intergenic 45.0%
CAAGAGAATCCATCGGGTAT+TGG + chr5_1:2788087-2788106 None:intergenic 45.0%
GAGATGCAAGAGAATCCATC+GGG + chr5_1:2788093-2788112 None:intergenic 45.0%
GGAGATGCAAGAGAATCCAT+CGG + chr5_1:2788094-2788113 None:intergenic 45.0%
! CTTCCAGCTTTGGTTTCTCA+AGG - chr5_1:2788379-2788398 Msa0710900:intron 45.0%
GAACCTTGAGAAACCAAAGC+TGG + chr5_1:2788385-2788404 None:intergenic 45.0%
TGTTCCTGGTCTGATTCACT+TGG + chr5_1:2788444-2788463 None:intergenic 45.0%
!!! CTTTTTGAGGCCAGGAACAA+TGG + chr5_1:2788545-2788564 None:intergenic 45.0%
CATTGTTCCTGGCCTCAAAA+AGG - chr5_1:2788543-2788562 Msa0710900:intron 45.0%
!!! GATCATACCTTTTTGAGGCC+AGG + chr5_1:2788553-2788572 None:intergenic 45.0%
GATGGACAAAGCTTCTGTGT+TGG - chr5_1:2789151-2789170 Msa0710900:intron 45.0%
CTTCGATTTCAGGAAGTGGT+TGG + chr5_1:2789334-2789353 None:intergenic 45.0%
GGAAAACTGTGTGACAGCAA+AGG - chr5_1:2790111-2790130 Msa0710900:CDS 45.0%
ATGAAGGAAGGTCCTGTGAT+GGG - chr5_1:2790304-2790323 Msa0710900:intron 45.0%
!! TTTAATAATGTATTATATAT+AGG - chr5_1:2789534-2789553 Msa0710900:intron 5.0%
AAGTTCAGGCACATAGGTGG+GGG + chr5_1:2787820-2787839 None:intergenic 50.0%
CAAGTTCAGGCACATAGGTG+GGG + chr5_1:2787821-2787840 None:intergenic 50.0%
GCTCTTTCAGCCATTGTTCC+TGG - chr5_1:2788532-2788551 Msa0710900:intron 50.0%
ACAAGCACGTTTGCAGACAC+TGG - chr5_1:2789202-2789221 Msa0710900:intron 50.0%
! TAATTGGCCAAGCACCGTAG+CGG + chr5_1:2789417-2789436 None:intergenic 50.0%
!!! TTTTTTGTGGTGGCCGAGGT+TGG - chr5_1:2789892-2789911 Msa0710900:CDS 50.0%
TGTGACAGCAAAGGATCTCC+TGG - chr5_1:2790120-2790139 Msa0710900:CDS 50.0%
GTGACAGCAAAGGATCTCCT+GGG - chr5_1:2790121-2790140 Msa0710900:CDS 50.0%
CATCACAGGACCTTCCTTCA+TGG + chr5_1:2790305-2790324 None:intergenic 50.0%
CATGAAGGAAGGTCCTGTGA+TGG - chr5_1:2790303-2790322 Msa0710900:intron 50.0%
TCGAGCTTGAGCAGTGTTCC+TGG + chr5_1:2788458-2788477 None:intergenic 55.0%
AGCACGTTTGCAGACACTGG+AGG - chr5_1:2789205-2789224 Msa0710900:intron 55.0%
! TCTCCTGGGAAGCATACGAC+AGG - chr5_1:2790135-2790154 Msa0710900:CDS 55.0%
AAGCCTGTCGTATGCTTCCC+AGG + chr5_1:2790141-2790160 None:intergenic 55.0%
! ACATGTTCTGGGTCGGTGTG+TGG - chr5_1:2790404-2790423 Msa0710900:intron 55.0%
! ACACAGTGGACGTGCCGCTA+CGG - chr5_1:2789400-2789419 Msa0710900:intron 60.0%
! GGACGTGCCGCTACGGTGCT+TGG - chr5_1:2789407-2789426 Msa0710900:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 2787522 2790732 2787522 ID=Msa0710900;Name=Msa0710900
chr5_1 mRNA 2787522 2790732 2787522 ID=Msa0710900-mRNA-1;Parent=Msa0710900;Name=Msa0710900-mRNA-1;_AED=0.06;_eAED=0.06;_QI=74|1|1|1|0.5|0.4|5|555|345
chr5_1 exon 2787522 2788151 2787522 ID=Msa0710900-mRNA-1:exon:931;Parent=Msa0710900-mRNA-1
chr5_1 exon 2788737 2789102 2788737 ID=Msa0710900-mRNA-1:exon:930;Parent=Msa0710900-mRNA-1
chr5_1 exon 2789690 2790196 2789690 ID=Msa0710900-mRNA-1:exon:929;Parent=Msa0710900-mRNA-1
chr5_1 exon 2790415 2790507 2790415 ID=Msa0710900-mRNA-1:exon:928;Parent=Msa0710900-mRNA-1
chr5_1 exon 2790662 2790732 2790662 ID=Msa0710900-mRNA-1:exon:927;Parent=Msa0710900-mRNA-1
chr5_1 five_prime_UTR 2790662 2790732 2790662 ID=Msa0710900-mRNA-1:five_prime_utr;Parent=Msa0710900-mRNA-1
chr5_1 five_prime_UTR 2790505 2790507 2790505 ID=Msa0710900-mRNA-1:five_prime_utr;Parent=Msa0710900-mRNA-1
chr5_1 CDS 2790415 2790504 2790415 ID=Msa0710900-mRNA-1:cds;Parent=Msa0710900-mRNA-1
chr5_1 CDS 2789690 2790196 2789690 ID=Msa0710900-mRNA-1:cds;Parent=Msa0710900-mRNA-1
chr5_1 CDS 2788737 2789102 2788737 ID=Msa0710900-mRNA-1:cds;Parent=Msa0710900-mRNA-1
chr5_1 CDS 2788077 2788151 2788077 ID=Msa0710900-mRNA-1:cds;Parent=Msa0710900-mRNA-1
chr5_1 three_prime_UTR 2787522 2788076 2787522 ID=Msa0710900-mRNA-1:three_prime_utr;Parent=Msa0710900-mRNA-1
Gene Sequence

>Msa0710900

ATGTCCCATACCACATCTAATCAAATCGAAGTTTTAGGAAAGAAACTGAAGATGATGCAAATTATGTCCCCCACCTATGTGCCTGAACTTGGAATGGAGGATCTCAACTTCTTTAACCAATACCCGATGGATTCTCTTGCATCTCCAATATTATTCGACAACTTTGGCTTTCACTTTGATGACAACGAAACCACACCAAATTGTTTCCAAGTTGAAACTCATCTTGATGATCAAACAAGGCCAACCAAGAAGATCAAAACATCGATAAACCCTTCTTCCTATTCATCACCTCAATTCATTTCTTTTGAACACTCCAGTTCAACACCAATTGCTTCTAAGCAATTTTATAACCTTGATTATTCCGATGTAAAACCTAAAGTCGAGAAAAGGTGTAATGATAACAAAGATTTTCTTCCAGCTTTGGTTTCTCAAGGTTCTTATGAAGATCAAAAAATATTTTCAAATTATGATAACCAAGTGAATCAGACCAGGAACACTGCTCAAGCTCGAGAACATGTAATGGCCGAAAGAAAACGAAGGGAAAAGCTTACCCGAAGTTTCATCGCTCTTTCAGCCATTGTTCCTGGCCTCAAAAAGATGGACAAAGCTTCTGTGTTGGGAGATGCTATCAAGTACATGAAACAACTACAAGCACGTTTGCAGACACTGGAGGAACAAGCTGAAGACAACAAAAAAACTGGATCCACAGTTCAAGTGAAGAGATCTATTATCTTCCCTAATGATGACGATGATGATGATGATAGCAACTCCAATAACCAACCACTTCCTGAAATCGAAGTAAGAGTATCAAGCAAAGATGTACTCATCAAGATTCAATGTGACAAACACAGTGGACGTGCCGCTACGGTGCTTGGCCAATTAGAAAACCTTAATCTCACTGTCCAAAGTAGTACCTTTTTGCCCTTTGGAAACAACATTGTTGATGTAACCATTGTTGCTAAGAGGAACAAGGAAAACTGTGTGACAGCAAAGGATCTCCTGGGAAGCATACGACAGGCTTTAATCATCCAAAATTAA
Protein sequence

>Msa0710900

MSHTTSNQIEVLGKKLKMMQIMSPTYVPELGMEDLNFFNQYPMDSLASPILFDNFGFHFDDNETTPNCFQVETHLDDQTRPTKKIKTSINPSSYSSPQFISFEHSSSTPIASKQFYNLDYSDVKPKVEKRCNDNKDFLPALVSQGSYEDQKIFSNYDNQVNQTRNTAQAREHVMAERKRREKLTRSFIALSAIVPGLKKMDKASVLGDAIKYMKQLQARLQTLEEQAEDNKKTGSTVQVKRSIIFPNDDDDDDDSNSNNQPLPEIEVRVSSKDVLIKIQCDKHSGRAATVLGQLENLNLTVQSSTFLPFGNNIVDVTIVAKRNKENCVTAKDLLGSIRQALIIQN*