AlfalfaGEDB | Msa0710900

M. sativa cultivar zhongmu-4 / Msa0710900

KEGG: -
GO: -
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0710900	XP_003611502.2	93.293	328	20	1	18	345	1	326	0.0	605

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0710900	sp\|Q9T072\|BH025_ARATH	35.988	339	186	13	19	341	1	324	3.65e-48	168

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0710900	G7K134	93.293	328	20	1	18	345	1	326	0.0	605

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TFs/TRs:

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Gene ID	Type	Classification
Msa0710900	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0710900	Msa0754590	0.865488	5.696916e-65	-8.615850e-47
Msa0710900	Msa0834800	0.851828	6.882622e-61	-8.615850e-47
Msa0710900	Msa1392850	0.867928	9.532276e-66	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0710900	MtrunA17_Chr5g0400671	93.293	328	20	1	18	345	1	326	0.0	605
Msa0710900	MtrunA17_Chr4g0054561	46.110	347	141	13	18	341	1	324	1.06e-80	249
Msa0710900	MtrunA17_Chr4g0054551	43.228	347	151	13	18	341	1	324	5.01e-75	234
Msa0710900	MtrunA17_Chr2g0280161	35.795	352	188	12	19	340	5	348	1.36e-52	177
Msa0710900	MtrunA17_Chr4g0049331	33.540	322	186	10	32	341	1	306	7.94e-48	163
Msa0710900	MtrunA17_Chr5g0400661	39.916	238	122	6	121	342	102	334	1.02e-45	159
Msa0710900	MtrunA17_Chr5g0400621	38.023	263	143	4	80	340	83	327	7.98e-45	156
Msa0710900	MtrunA17_Chr4g0002781	43.750	192	98	3	158	342	161	349	2.54e-43	153
Msa0710900	MtrunA17_Chr4g0002801	39.823	226	117	4	133	342	108	330	3.32e-42	149
Msa0710900	MtrunA17_Chr2g0333621	36.047	258	139	6	92	342	73	311	3.94e-42	149

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0710900	AT4G37850.2	35.988	339	186	13	19	341	1	324	3.71e-49	168
Msa0710900	AT4G37850.1	35.988	339	186	13	19	341	1	324	3.71e-49	168
Msa0710900	AT2G22760.1	43.784	185	99	2	163	342	111	295	1.34e-45	157
Msa0710900	AT2G22760.2	46.012	163	84	1	163	321	111	273	4.06e-43	150
Msa0710900	AT2G22750.3	33.819	343	153	14	19	323	5	311	3.43e-42	150
Msa0710900	AT2G22750.5	33.819	343	153	14	19	323	1	307	3.66e-42	149
Msa0710900	AT2G22750.4	33.819	343	153	14	19	323	5	311	1.16e-41	148
Msa0710900	AT2G22750.2	45.763	177	69	4	163	323	118	283	2.74e-39	141
Msa0710900	AT2G22750.1	45.763	177	69	4	163	323	118	283	9.14e-39	140
Msa0710900	AT2G22770.1	39.810	211	109	3	149	341	108	318	7.07e-37	135

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Find 91 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAAATTAGAAGAGAAAG+TGG	0.442399	5_1:+2790677	None:intergenic
AACCCTAGAATCCTTATCAA+AGG	0.573151	5_1:+2787960	None:intergenic
AACTTCGATTTGATTAGATG+TGG	0.560569	5_1:+2790472	None:intergenic
AACTTGGAAACAATTTGGTG+TGG	0.596690	5_1:+2790074	None:intergenic
AAGCCTGTCGTATGCTTCCC+AGG	0.497417	5_1:+2788094	None:intergenic
AAGTTCAGGCACATAGGTGG+GGG	0.650925	5_1:+2790415	None:intergenic
AATAATCACAAAACGACAAG+AGG	0.560394	5_1:+2787562	None:intergenic
AATGAATTGAGGTGATGAAT+AGG	0.381227	5_1:+2789987	None:intergenic
AATGGCCGAAAGAAAACGAA+GGG	0.657033	5_1:-2789746	Msa0710900:CDS
AATTCTCTTGCAGAGGAACA+AGG	0.562972	5_1:-2788142	Msa0710900:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GATTTGATTAGATGTGGTAT+GGG	+	chr5_1:2787757-2787776	None:intergenic	30.0%
CCAAAGTTGTCGAATAATAT+TGG	+	chr5_1:2788115-2788134	None:intergenic	30.0%
CCAATATTATTCGACAACTT+TGG	-	chr5_1:2788112-2788131	Msa0710900:CDS	30.0%
GTTTCAACTTGGAAACAATT+TGG	+	chr5_1:2788166-2788185	None:intergenic	30.0%
AATGAATTGAGGTGATGAAT+AGG	+	chr5_1:2788248-2788267	None:intergenic	30.0%
TAAAACCTAAAGTCGAGAAA+AGG	-	chr5_1:2788335-2788354	Msa0710900:intron	30.0%
ATCCATATTAAAGGCTGAAA+TGG	-	chr5_1:2788660-2788679	Msa0710900:intron	30.0%
AATGGTCACTAATATTGCTA+GGG	+	chr5_1:2788709-2788728	None:intergenic	30.0%
AAATGGTCACTAATATTGCT+AGG	+	chr5_1:2788710-2788729	None:intergenic	30.0%
AAGAGTAGACATCAAAAAGT+TGG	-	chr5_1:2788845-2788864	Msa0710900:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	2787522	2790732	2787522	ID=Msa0710900;Name=Msa0710900
chr5_1	mRNA	2787522	2790732	2787522	ID=Msa0710900-mRNA-1;Parent=Msa0710900;Name=Msa0710900-mRNA-1;_AED=0.06;_eAED=0.06;_QI=74\|1\|1\|1\|0.5\|0.4\|5\|555\|345
chr5_1	exon	2787522	2788151	2787522	ID=Msa0710900-mRNA-1:exon:931;Parent=Msa0710900-mRNA-1
chr5_1	exon	2788737	2789102	2788737	ID=Msa0710900-mRNA-1:exon:930;Parent=Msa0710900-mRNA-1
chr5_1	exon	2789690	2790196	2789690	ID=Msa0710900-mRNA-1:exon:929;Parent=Msa0710900-mRNA-1
chr5_1	exon	2790415	2790507	2790415	ID=Msa0710900-mRNA-1:exon:928;Parent=Msa0710900-mRNA-1
chr5_1	exon	2790662	2790732	2790662	ID=Msa0710900-mRNA-1:exon:927;Parent=Msa0710900-mRNA-1
chr5_1	five_prime_UTR	2790662	2790732	2790662	ID=Msa0710900-mRNA-1:five_prime_utr;Parent=Msa0710900-mRNA-1
chr5_1	five_prime_UTR	2790505	2790507	2790505	ID=Msa0710900-mRNA-1:five_prime_utr;Parent=Msa0710900-mRNA-1
chr5_1	CDS	2790415	2790504	2790415	ID=Msa0710900-mRNA-1:cds;Parent=Msa0710900-mRNA-1
chr5_1	CDS	2789690	2790196	2789690	ID=Msa0710900-mRNA-1:cds;Parent=Msa0710900-mRNA-1
chr5_1	CDS	2788737	2789102	2788737	ID=Msa0710900-mRNA-1:cds;Parent=Msa0710900-mRNA-1
chr5_1	CDS	2788077	2788151	2788077	ID=Msa0710900-mRNA-1:cds;Parent=Msa0710900-mRNA-1
chr5_1	three_prime_UTR	2787522	2788076	2787522	ID=Msa0710900-mRNA-1:three_prime_utr;Parent=Msa0710900-mRNA-1

Gene Sequence

>Msa0710900

ATGTCCCATACCACATCTAATCAAATCGAAGTTTTAGGAAAGAAACTGAAGATGATGCAAATTATGTCCCCCACCTATGTGCCTGAACTTGGAATGGAGGATCTCAACTTCTTTAACCAATACCCGATGGATTCTCTTGCATCTCCAATATTATTCGACAACTTTGGCTTTCACTTTGATGACAACGAAACCACACCAAATTGTTTCCAAGTTGAAACTCATCTTGATGATCAAACAAGGCCAACCAAGAAGATCAAAACATCGATAAACCCTTCTTCCTATTCATCACCTCAATTCATTTCTTTTGAACACTCCAGTTCAACACCAATTGCTTCTAAGCAATTTTATAACCTTGATTATTCCGATGTAAAACCTAAAGTCGAGAAAAGGTGTAATGATAACAAAGATTTTCTTCCAGCTTTGGTTTCTCAAGGTTCTTATGAAGATCAAAAAATATTTTCAAATTATGATAACCAAGTGAATCAGACCAGGAACACTGCTCAAGCTCGAGAACATGTAATGGCCGAAAGAAAACGAAGGGAAAAGCTTACCCGAAGTTTCATCGCTCTTTCAGCCATTGTTCCTGGCCTCAAAAAGATGGACAAAGCTTCTGTGTTGGGAGATGCTATCAAGTACATGAAACAACTACAAGCACGTTTGCAGACACTGGAGGAACAAGCTGAAGACAACAAAAAAACTGGATCCACAGTTCAAGTGAAGAGATCTATTATCTTCCCTAATGATGACGATGATGATGATGATAGCAACTCCAATAACCAACCACTTCCTGAAATCGAAGTAAGAGTATCAAGCAAAGATGTACTCATCAAGATTCAATGTGACAAACACAGTGGACGTGCCGCTACGGTGCTTGGCCAATTAGAAAACCTTAATCTCACTGTCCAAAGTAGTACCTTTTTGCCCTTTGGAAACAACATTGTTGATGTAACCATTGTTGCTAAGAGGAACAAGGAAAACTGTGTGACAGCAAAGGATCTCCTGGGAAGCATACGACAGGCTTTAATCATCCAAAATTAA

Protein sequence

>Msa0710900

MSHTTSNQIEVLGKKLKMMQIMSPTYVPELGMEDLNFFNQYPMDSLASPILFDNFGFHFDDNETTPNCFQVETHLDDQTRPTKKIKTSINPSSYSSPQFISFEHSSSTPIASKQFYNLDYSDVKPKVEKRCNDNKDFLPALVSQGSYEDQKIFSNYDNQVNQTRNTAQAREHVMAERKRREKLTRSFIALSAIVPGLKKMDKASVLGDAIKYMKQLQARLQTLEEQAEDNKKTGSTVQVKRSIIFPNDDDDDDDSNSNNQPLPEIEVRVSSKDVLIKIQCDKHSGRAATVLGQLENLNLTVQSSTFLPFGNNIVDVTIVAKRNKENCVTAKDLLGSIRQALIIQN*