AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1392850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1392850 MtrunA17_Chr5g0400671 94.207 328 17 1 18 345 1 326 0.0 610
Msa1392850 MtrunA17_Chr4g0054561 47.175 354 127 15 18 341 1 324 8.53e-82 251
Msa1392850 MtrunA17_Chr4g0054551 44.350 354 137 15 18 341 1 324 5.41e-76 237
Msa1392850 MtrunA17_Chr2g0280161 35.328 351 191 11 19 340 5 348 7.40e-53 178
Msa1392850 MtrunA17_Chr4g0049331 33.851 322 185 10 32 341 1 306 1.68e-49 168
Msa1392850 MtrunA17_Chr5g0400661 39.916 238 122 6 121 342 102 334 4.48e-46 159
Msa1392850 MtrunA17_Chr5g0400621 38.023 263 143 4 80 340 83 327 5.46e-45 157
Msa1392850 MtrunA17_Chr4g0002781 38.168 262 131 7 91 342 109 349 3.07e-44 155
Msa1392850 MtrunA17_Chr2g0333621 36.047 258 139 6 92 342 73 311 1.58e-42 150
Msa1392850 MtrunA17_Chr4g0002801 39.823 226 117 4 133 342 108 330 2.28e-42 150
Msa1392850 MtrunA17_Chr4g0033391 41.436 181 100 2 164 342 140 316 4.12e-41 146
Msa1392850 MtrunA17_Chr2g0333611 40.000 185 102 4 162 342 36 215 2.51e-40 141
Msa1392850 MtrunA17_Chr8g0336681 39.153 189 103 3 164 342 149 335 1.40e-39 142
Msa1392850 MtrunA17_Chr7g0225901 45.161 155 84 1 168 321 85 239 3.08e-39 139
Msa1392850 MtrunA17_Chr2g0307131 41.489 188 104 2 158 339 161 348 8.74e-39 141
Msa1392850 MtrunA17_Chr4g0033291 41.262 206 104 4 143 342 130 324 2.64e-38 139
Msa1392850 MtrunA17_Chr6g0468491 41.379 174 101 1 166 338 132 305 2.81e-38 138
Msa1392850 MtrunA17_Chr2g0307141 43.429 175 92 3 169 339 181 352 4.47e-38 139
Msa1392850 MtrunA17_Chr4g0054581 40.201 199 103 6 155 342 181 374 1.59e-37 138
Msa1392850 MtrunA17_Chr7g0249811 39.665 179 100 1 164 342 158 328 1.49e-36 134
Msa1392850 MtrunA17_Chr5g0400641 44.079 152 84 1 174 324 1 152 2.09e-36 129
Msa1392850 MtrunA17_Chr2g0333641 34.263 251 139 6 104 341 69 306 5.04e-36 132
Msa1392850 MtrunA17_Chr4g0033281 38.308 201 116 4 143 342 128 321 3.30e-35 130
Msa1392850 MtrunA17_Chr2g0333541 40.860 186 103 3 164 344 87 270 1.06e-32 123
Msa1392850 MtrunA17_Chr4g0054621 35.263 190 114 3 155 342 155 337 1.62e-32 124
Msa1392850 MtrunA17_Chr4g0054571 37.500 192 106 2 162 342 141 329 1.15e-31 121
Msa1392850 MtrunA17_Chr6g0468461 40.385 156 80 1 166 321 168 310 3.28e-30 118
Msa1392850 MtrunA17_Chr4g0002851 35.762 151 80 1 194 344 65 198 1.75e-20 88.2
Msa1392850 MtrunA17_Chr8g0366751 36.196 163 70 5 164 301 451 604 3.99e-20 91.7
Msa1392850 MtrunA17_Chr7g0233441 33.775 151 91 1 194 344 57 198 3.25e-19 84.3
Msa1392850 MtrunA17_Chr4g0002831 31.098 164 102 3 182 344 53 206 1.73e-18 82.8
Msa1392850 MtrunA17_Chr1g0185621 33.333 183 104 5 172 341 476 653 1.90e-18 86.7
Msa1392850 MtrunA17_Chr7g0275821 28.729 181 106 2 172 344 253 418 9.09e-18 84.0
Msa1392850 MtrunA17_Chr5g0411341 34.810 158 87 2 164 305 482 639 1.19e-17 84.3
Msa1392850 MtrunA17_Chr7g0252041 30.481 187 110 4 171 343 286 466 2.38e-16 79.7
Msa1392850 MtrunA17_Chr8g0346161 30.058 173 116 2 172 339 292 464 2.07e-15 77.0
Msa1392850 MtrunA17_Chr8g0346151 36.029 136 71 4 172 295 280 411 2.78e-15 76.6
Msa1392850 MtrunA17_Chr8g0365711 25.146 171 122 2 155 319 228 398 9.28e-15 74.7
Msa1392850 MtrunA17_Chr7g0260841 34.146 123 54 3 162 280 342 441 4.91e-13 69.7
Msa1392850 MtrunA17_Chr3g0131691 29.560 159 97 4 171 316 298 454 1.06e-12 68.9
Msa1392850 MtrunA17_Chr1g0211081 32.090 134 82 3 103 235 256 381 1.61e-12 68.2
Msa1392850 MtrunA17_Chr5g0411541 26.596 282 170 9 85 342 47 315 1.95e-12 67.4
Msa1392850 MtrunA17_Chr4g0028071 31.206 141 82 4 173 304 151 285 2.10e-12 67.4
Msa1392850 MtrunA17_Chr8g0386921 28.313 166 83 4 172 304 407 569 3.39e-12 67.4
Msa1392850 MtrunA17_Chr2g0320291 30.714 140 84 4 173 306 135 267 2.26e-11 63.9
Msa1392850 MtrunA17_Chr3g0141091 29.379 177 106 4 167 342 169 327 2.77e-11 63.9
Msa1392850 MtrunA17_Chr2g0298751 24.352 193 130 5 149 326 29 220 3.34e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1392850 AT4G37850.2 35.988 339 186 13 19 341 1 324 4.18e-49 167
Msa1392850 AT4G37850.1 35.988 339 186 13 19 341 1 324 4.18e-49 167
Msa1392850 AT2G22760.1 43.784 185 99 2 163 342 111 295 8.85e-46 158
Msa1392850 AT2G22760.2 46.012 163 84 1 163 321 111 273 2.37e-43 151
Msa1392850 AT2G22750.5 33.819 343 153 14 19 323 1 307 3.44e-42 149
Msa1392850 AT2G22750.3 33.819 343 153 14 19 323 5 311 3.54e-42 149
Msa1392850 AT2G22750.4 33.819 343 153 14 19 323 5 311 1.09e-41 148
Msa1392850 AT2G22750.2 45.763 177 69 4 163 323 118 283 1.69e-39 142
Msa1392850 AT2G22750.1 45.763 177 69 4 163 323 118 283 4.07e-39 140
Msa1392850 AT2G22770.1 39.810 211 109 3 149 341 108 318 5.92e-37 135
Msa1392850 AT4G17880.1 29.675 246 127 8 90 306 324 552 4.55e-20 91.7
Msa1392850 AT5G46760.1 34.783 161 78 4 164 306 404 555 4.98e-19 88.6
Msa1392850 AT1G32640.1 35.099 151 92 3 162 306 439 589 1.01e-16 81.6
Msa1392850 AT2G46510.1 26.667 180 111 3 162 337 382 544 3.38e-16 80.1
Msa1392850 AT1G01260.1 35.099 151 64 4 167 307 429 555 4.92e-16 79.7
Msa1392850 AT1G01260.2 35.099 151 64 4 167 307 429 555 4.92e-16 79.7
Msa1392850 AT1G01260.3 35.099 151 64 4 167 307 429 555 4.92e-16 79.7
Msa1392850 AT5G57150.4 26.455 189 119 3 149 322 33 216 1.66e-15 75.9
Msa1392850 AT5G57150.5 26.344 186 117 3 149 319 32 212 2.25e-15 74.7
Msa1392850 AT5G57150.3 26.344 186 117 3 149 319 33 213 3.09e-15 74.3
Msa1392850 AT5G57150.2 26.344 186 117 3 149 319 33 213 3.13e-15 74.3
Msa1392850 AT5G57150.1 26.154 195 124 3 149 328 32 221 3.18e-15 74.7
Msa1392850 AT1G12860.1 29.444 180 115 4 171 343 267 441 1.05e-14 75.1
Msa1392850 AT1G12860.2 31.373 153 98 3 171 316 267 419 1.27e-14 74.7
Msa1392850 AT4G09820.1 34.884 129 63 2 172 299 364 472 1.98e-14 74.7
Msa1392850 AT2G16910.1 27.684 177 105 4 172 325 315 491 8.41e-14 72.8
Msa1392850 AT1G63650.3 29.082 196 101 7 172 341 406 589 1.63e-13 72.0
Msa1392850 AT1G63650.1 29.082 196 101 7 172 341 406 589 1.63e-13 72.0
Msa1392850 AT1G63650.2 29.082 196 101 7 172 341 406 589 1.63e-13 72.0
Msa1392850 AT4G29930.5 30.345 145 88 3 172 313 55 189 2.67e-13 68.9
Msa1392850 AT4G29930.2 30.345 145 88 3 172 313 55 189 3.64e-13 68.6
Msa1392850 AT4G29930.6 30.822 146 89 3 172 313 55 192 3.73e-13 68.6
Msa1392850 AT3G26744.1 29.143 175 108 4 171 334 307 476 3.42e-12 67.8
Msa1392850 AT3G26744.4 29.143 175 108 4 171 334 307 476 3.42e-12 67.8
Msa1392850 AT3G26744.2 29.143 175 108 4 171 334 307 476 3.42e-12 67.8
Msa1392850 AT5G65640.1 28.889 180 112 5 167 342 174 341 3.77e-12 67.0
Msa1392850 AT3G26744.6 30.573 157 98 3 171 316 307 463 5.86e-12 67.0
Msa1392850 AT4G16430.1 45.333 75 37 1 162 232 307 381 6.49e-12 66.6
Msa1392850 AT4G00870.1 29.927 137 92 1 172 304 250 386 6.88e-12 66.6
Msa1392850 AT4G29930.3 26.829 164 98 3 172 313 55 218 1.79e-11 64.3
Msa1392850 AT4G29930.7 26.829 164 98 3 172 313 55 218 1.79e-11 64.3
Msa1392850 AT5G41315.3 28.788 198 104 8 172 341 447 635 2.29e-11 65.5
Msa1392850 AT5G41315.2 28.788 198 104 8 172 341 450 638 2.34e-11 65.5
Msa1392850 AT5G41315.1 28.788 198 104 8 172 341 442 630 2.43e-11 65.5
Msa1392850 AT4G29930.1 26.829 164 98 3 172 313 55 218 2.56e-11 63.5

Find 91 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTCAACTGGTTAATTT+TGG 0.110602 tig0021533:+70041 None:intergenic
TTTCTCATCTTACACCAATT+TGG 0.189336 tig0021533:-72740 None:intergenic
GATACTCTTACTTCGATTTC+AGG 0.236620 tig0021533:+70872 None:intergenic
GTTTCAACTGGGAAACAATT+TGG 0.250112 tig0021533:+72091 None:intergenic
TACTTTGGACAGTGAGATTA+AGG 0.260165 tig0021533:+70771 None:intergenic
TTCAGGAAGTGGTTGGTTAT+TGG 0.276191 tig0021533:+70889 None:intergenic
TCCTGTTGTGAAAAGGTTCT+AGG 0.284232 tig0021533:-69607 Msa1392850:three_prime_UTR
TATCGATGTCTTGATCTTCT+TGG 0.286833 tig0021533:+72042 None:intergenic
GCTCTTTCAGCCATTGTTCC+TGG 0.288584 tig0021533:-71722 Msa1392850:CDS
CCAAAGTTGTCGAATAATAT+TGG 0.304765 tig0021533:+72142 None:intergenic
TGTCCTTTGATAAGGATTCT+AGG 0.308395 tig0021533:-69944 Msa1392850:three_prime_UTR
TCGAGCTTGAGCAGTGTTCC+TGG 0.329696 tig0021533:+71799 None:intergenic
AGAGAGTTAATATTCCAAAT+TGG 0.339623 tig0021533:+72726 None:intergenic
CCTTCTTTCCTGTTGTGAAA+AGG 0.358968 tig0021533:-69614 Msa1392850:three_prime_UTR
GATTAAAATATTCCTTCAAC+TGG 0.373209 tig0021533:+70031 None:intergenic
CATCACAGGACCTTCCTTCA+TGG 0.375414 tig0021533:+69911 None:intergenic
CGATTTGATTAGATGTGGTA+TGG 0.375743 tig0021533:+72499 None:intergenic
TAAAATTTCTTAGAAGCAAT+TGG 0.377682 tig0021533:+71962 None:intergenic
CATTGTTCCTGGCCTCAAAA+AGG 0.384959 tig0021533:-71711 Msa1392850:intron
TCATCAAGATGAGTTTCAAC+TGG 0.389714 tig0021533:+72079 None:intergenic
CTTCCAGCTTTGGTTTCTCA+AGG 0.393979 tig0021533:-71875 Msa1392850:CDS
AATGAGTTGAGGTGATGAAT+AGG 0.399012 tig0021533:+72009 None:intergenic
GTTGTTTCCAAAGGGCAAAA+AGG 0.400410 tig0021533:+70745 None:intergenic
CCAATATTATTCGACAACTT+TGG 0.407380 tig0021533:-72142 Msa1392850:CDS
AGTAGTATGCTTACAAGTTC+AGG 0.414356 tig0021533:+72423 None:intergenic
AGAAGCAATTGGTGTTGAAC+TGG 0.417121 tig0021533:+71973 None:intergenic
TGAGGTGATGAATAGGAAGA+AGG 0.434612 tig0021533:+72016 None:intergenic
ATGGACAAAGCTTCTGTGTT+GGG 0.439391 tig0021533:-71061 Msa1392850:CDS
AAATAAATTAGAAGAGAAAG+TGG 0.442399 tig0021533:+72696 None:intergenic
TGTGACAGCAAAGGATCTCC+TGG 0.446189 tig0021533:-70089 Msa1392850:CDS
CAAGAGAATCCATCGGGTAT+TGG 0.448559 tig0021533:+72170 None:intergenic
GTCCTTTGATAAGGATTCTA+GGG 0.456194 tig0021533:-69943 Msa1392850:three_prime_UTR
TGAAAGAGCGATGAAACTTC+GGG 0.456547 tig0021533:+71736 None:intergenic
ATCCAAAATTAACCAGTTGA+AGG 0.460869 tig0021533:-70043 Msa1392850:exon
GAGGTGATGAATAGGAAGAA+GGG 0.466954 tig0021533:+72017 None:intergenic
GGACGTGCCGCTACGGTGCT+TGG 0.467945 tig0021533:-70806 Msa1392850:CDS
ATCAACAATGTTGTTTCCAA+AGG 0.469768 tig0021533:+70736 None:intergenic
TCAACAATGTTGTTTCCAAA+GGG 0.475298 tig0021533:+70737 None:intergenic
GATGTCTTGATCTTCTTGGT+TGG 0.487867 tig0021533:+72046 None:intergenic
CTGAAAGAGCGATGAAACTT+CGG 0.488970 tig0021533:+71735 None:intergenic
AAGCCTGTCGTATGCTTCCC+AGG 0.497417 tig0021533:+70071 None:intergenic
TTCTACAACATGTTCTGGGT+CGG 0.501517 tig0021533:-69816 Msa1392850:three_prime_UTR
ATTAACAATTACACATGTGA+TGG 0.502857 tig0021533:+69703 None:intergenic
CTTCGATTTCAGGAAGTGGT+TGG 0.508259 tig0021533:+70882 None:intergenic
TAAAACCTAAAGTCGAGAAA+AGG 0.508413 tig0021533:-71919 Msa1392850:CDS
TGTTCCTGGTCTGATTCACT+TGG 0.510038 tig0021533:+71813 None:intergenic
CTTACTTCGATTTCAGGAAG+TGG 0.529255 tig0021533:+70878 None:intergenic
TTTATTTACCTTAGCAACAA+TGG 0.529541 tig0021533:+70709 None:intergenic
GATTTGATTAGATGTGGTAT+GGG 0.531705 tig0021533:+72500 None:intergenic
GAACCTTGAGAAACCAAAGC+TGG 0.540922 tig0021533:+71872 None:intergenic
TGATGTAACCATTGTTGCTA+AGG 0.545162 tig0021533:-70717 Msa1392850:intron
ACAAGTTCAGGCACATAGGT+GGG 0.548457 tig0021533:+72435 None:intergenic
ATCATAGATTCTCCCATCAC+AGG 0.549532 tig0021533:+69897 None:intergenic
ACAAGCACGTTTGCAGACAC+TGG 0.553923 tig0021533:-71011 Msa1392850:CDS
TCTAGGTTTGATAGAAAATG+TGG 0.554779 tig0021533:-69590 Msa1392850:three_prime_UTR
AATAATCACAAAACGACAAG+AGG 0.560394 tig0021533:+69541 None:intergenic
AACTTCGATTTGATTAGATG+TGG 0.560569 tig0021533:+72494 None:intergenic
ATGAAGGAAGGTCCTGTGAT+GGG 0.560852 tig0021533:-69909 Msa1392850:three_prime_UTR
ACACAGTGGACGTGCCGCTA+CGG 0.562208 tig0021533:-70813 Msa1392850:CDS
AATTCTCTTGCAGAGGAACA+AGG 0.562972 tig0021533:-70119 Msa1392850:intron
AACCCTAGAATCCTTATCAA+AGG 0.573151 tig0021533:+69941 None:intergenic
CAAGTTCAGGCACATAGGTG+GGG 0.574321 tig0021533:+72436 None:intergenic
CTTCTTTAACCAATACCCGA+TGG 0.574424 tig0021533:-72179 Msa1392850:CDS
TTTGCATAGTTGACTTGACT+AGG 0.577273 tig0021533:-69741 Msa1392850:three_prime_UTR
ACATGTTCTGGGTCGGTGTG+TGG 0.580330 tig0021533:-69809 Msa1392850:three_prime_UTR
CATGAAGGAAGGTCCTGTGA+TGG 0.581540 tig0021533:-69910 Msa1392850:three_prime_UTR
CAGGAAAGAAGGAAGAAAAG+AGG 0.581719 tig0021533:+69625 None:intergenic
AACTGGGAAACAATTTGGTG+TGG 0.582222 tig0021533:+72096 None:intergenic
TAGGGTTACTATATCCATGA+AGG 0.583367 tig0021533:-69925 Msa1392850:three_prime_UTR
TCAAGCTCGAGAACATGTAA+TGG 0.585662 tig0021533:-71786 Msa1392850:CDS
TACAAGTTCAGGCACATAGG+TGG 0.586428 tig0021533:+72434 None:intergenic
GAGATGCAAGAGAATCCATC+GGG 0.590902 tig0021533:+72164 None:intergenic
GCTTACAAGTTCAGGCACAT+AGG 0.592149 tig0021533:+72431 None:intergenic
CTCATCTTGATGATCAAACA+AGG 0.597867 tig0021533:-72069 Msa1392850:CDS
CATCAAGATGAGTTTCAACT+GGG 0.598938 tig0021533:+72080 None:intergenic
GTGTGTGGAATTTCAACAGA+TGG 0.598947 tig0021533:-69794 Msa1392850:three_prime_UTR
TCTCCTGGGAAGCATACGAC+AGG 0.600421 tig0021533:-70074 Msa1392850:CDS
GATGGACAAAGCTTCTGTGT+TGG 0.607222 tig0021533:-71062 Msa1392850:CDS
GTGACAGCAAAGGATCTCCT+GGG 0.607386 tig0021533:-70088 Msa1392850:CDS
AGATCTCTTCACTTGAACTG+TGG 0.609751 tig0021533:+70955 None:intergenic
GTTACTATATCCATGAAGGA+AGG 0.623815 tig0021533:-69921 Msa1392850:three_prime_UTR
GGAAAACTGTGTGACAGCAA+AGG 0.624039 tig0021533:-70098 Msa1392850:CDS
GGAGATGCAAGAGAATCCAT+CGG 0.627162 tig0021533:+72163 None:intergenic
TGTTCAAAAGAAATGAGTTG+AGG 0.643051 tig0021533:+71998 None:intergenic
AAGTTCAGGCACATAGGTGG+GGG 0.650925 tig0021533:+72437 None:intergenic
AATGGCCGAAAGAAAACGAA+GGG 0.657033 tig0021533:-71768 Msa1392850:CDS
TAATTGGCCAAGCACCGTAG+CGG 0.678187 tig0021533:+70799 None:intergenic
AGCACGTTTGCAGACACTGG+AGG 0.686426 tig0021533:-71008 Msa1392850:CDS
ATAACCAAGTGAATCAGACC+AGG 0.687464 tig0021533:-71817 Msa1392850:CDS
TAATGGCCGAAAGAAAACGA+AGG 0.695210 tig0021533:-71769 Msa1392850:CDS
ATTCAATGTGACAAACACAG+TGG 0.762327 tig0021533:-70827 Msa1392850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATAATTTATTTTTCTTT+TGG - tig0021533:70717-70736 Msa1392850:intron 10.0%
!! AATTAATGTATTCAATATTA+TGG - tig0021533:71137-71156 Msa1392850:intron 10.0%
!! AAAATAAATTAACATTTCTA+AGG - tig0021533:71605-71624 Msa1392850:intron 10.0%
!!! AAAAAATCATAAATTTTAGA+CGG - tig0021533:71848-71867 Msa1392850:CDS 10.0%
!!! TAATTTTTCTAATCATTATT+TGG - tig0021533:72546-72565 Msa1392850:intron 10.0%
!!! AAGTTTATGAATTTTAAGAT+AGG + tig0021533:69840-69859 None:intergenic 15.0%
!!! TTGTTTTTATTTCATTTTCT+AGG - tig0021533:70923-70942 Msa1392850:CDS 15.0%
!! TACAATATAAATATTGCAAT+TGG + tig0021533:71100-71119 None:intergenic 15.0%
!! TATAGGAAAATATTACATAT+AGG - tig0021533:71568-71587 Msa1392850:intron 15.0%
!!! AAATTTTGAAATGAAAAATG+TGG - tig0021533:71871-71890 Msa1392850:CDS 15.0%
!!! TTTTTTTTTTTTTTTTGTGG+TGG - tig0021533:71903-71922 Msa1392850:CDS 15.0%
!! TTTATGAATTATGATAATCT+AGG + tig0021533:72087-72106 None:intergenic 15.0%
!!! TTATGATTCATTTATTTTCA+AGG - tig0021533:72382-72401 Msa1392850:intron 15.0%
!!! TATGATTCATTTATTTTCAA+GGG - tig0021533:72383-72402 Msa1392850:intron 15.0%
!! TACAATATTAAAAAAAGAAG+TGG + tig0021533:72669-72688 None:intergenic 15.0%
!! AAATAAATTAGAAGAGAAAG+TGG + tig0021533:69537-69556 None:intergenic 20.0%
!!! TTTATTTTCTTCAATCTTCA+AGG - tig0021533:69550-69569 Msa1392850:three_prime_UTR 20.0%
!! AAGCAGATTAAGAATTAAAT+AGG + tig0021533:69579-69598 None:intergenic 20.0%
!!! TTAATCTGCTTTTTCTTTTA+GGG - tig0021533:69588-69607 Msa1392850:three_prime_UTR 20.0%
!!! TCTTTAATTACTTTTGAACA+GGG - tig0021533:70013-70032 Msa1392850:three_prime_UTR 20.0%
!! TAAAATTTCTTAGAAGCAAT+TGG + tig0021533:70271-70290 None:intergenic 20.0%
!! ACATTAATGATCCATATTAA+AGG - tig0021533:70627-70646 Msa1392850:intron 20.0%
!!! TATTTTCTTATGATCAGATT+AGG + tig0021533:70786-70805 None:intergenic 20.0%
!!! TTTTCTAATCATTATTTGGA+TGG - tig0021533:72550-72569 Msa1392850:intron 20.0%
! AGAGAGTTAATATTCCAAAT+TGG + tig0021533:69507-69526 None:intergenic 25.0%
!!! CTTAATCTGCTTTTTCTTTT+AGG - tig0021533:69587-69606 Msa1392850:three_prime_UTR 25.0%
! GTTGAAATCTTAGAATTGAA+GGG + tig0021533:69667-69686 None:intergenic 25.0%
! AGTTGAAATCTTAGAATTGA+AGG + tig0021533:69668-69687 None:intergenic 25.0%
!! TCTAATCAAATCGAAGTTTT+AGG - tig0021533:69741-69760 Msa1392850:three_prime_UTR 25.0%
!!! CTCTTTAATTACTTTTGAAC+AGG - tig0021533:70012-70031 Msa1392850:three_prime_UTR 25.0%
! ATTATAACATGTTTGTGAGT+TGG + tig0021533:70558-70577 None:intergenic 25.0%
! TGATATAGAAATGGTTTGAA+TGG + tig0021533:70587-70606 None:intergenic 25.0%
! GTAGTATGTTGATATAGAAA+TGG + tig0021533:70596-70615 None:intergenic 25.0%
! TATTGACGAAAAACATGAAA+TGG + tig0021533:70703-70722 None:intergenic 25.0%
!! TTAGTCTTCGAGAAAAAAAT+AGG - tig0021533:70816-70835 Msa1392850:CDS 25.0%
! TTGGTAAGTTCAACTTAATT+AGG + tig0021533:71081-71100 None:intergenic 25.0%
!! TGAGATTAAGGTTTTCTAAT+TGG + tig0021533:71450-71469 None:intergenic 25.0%
! TTTATTTACCTTAGCAACAA+TGG + tig0021533:71524-71543 None:intergenic 25.0%
!!! TAACATTTCTAAGGTTGTTT+AGG - tig0021533:71614-71633 Msa1392850:intron 25.0%
! GCATACTTGAACATAATAAA+CGG - tig0021533:71766-71785 Msa1392850:CDS 25.0%
! AAATATTAATTCTCTTGCAG+AGG - tig0021533:72104-72123 Msa1392850:CDS 25.0%
! GATTAAAATATTCCTTCAAC+TGG + tig0021533:72202-72221 None:intergenic 25.0%
! ATTAACAATTACACATGTGA+TGG + tig0021533:72530-72549 None:intergenic 25.0%
!!! TTTTTTGTCTTCGAACATGT+TGG - tig0021533:69624-69643 Msa1392850:three_prime_UTR 30.0%
GATTTGATTAGATGTGGTAT+GGG + tig0021533:69733-69752 None:intergenic 30.0%
! AACTTCGATTTGATTAGATG+TGG + tig0021533:69739-69758 None:intergenic 30.0%
!!! TTGACCAAGCTTTATTTTTC+TGG - tig0021533:69934-69953 Msa1392850:three_prime_UTR 30.0%
!! TTATTTTTCTGGTCGAACTT+TGG - tig0021533:69945-69964 Msa1392850:three_prime_UTR 30.0%
!! AATTACTTTTGAACAGGGAA+TGG - tig0021533:70018-70037 Msa1392850:three_prime_UTR 30.0%
CCAAAGTTGTCGAATAATAT+TGG + tig0021533:70091-70110 None:intergenic 30.0%
CCAATATTATTCGACAACTT+TGG - tig0021533:70088-70107 Msa1392850:CDS 30.0%
TGTTCAAAAGAAATGAGTTG+AGG + tig0021533:70235-70254 None:intergenic 30.0%
! GTTTTACATCGGAATAATCA+AGG + tig0021533:70297-70316 None:intergenic 30.0%
TAAAACCTAAAGTCGAGAAA+AGG - tig0021533:70311-70330 Msa1392850:intron 30.0%
! GATAAGATCATACCTTTTTG+AGG + tig0021533:70534-70553 None:intergenic 30.0%
ATCCATATTAAAGGCTGAAA+TGG - tig0021533:70636-70655 Msa1392850:intron 30.0%
AATGGTCACTAATATTGCTA+GGG + tig0021533:70685-70704 None:intergenic 30.0%
AAATGGTCACTAATATTGCT+AGG + tig0021533:70686-70705 None:intergenic 30.0%
AAGAGTAGACATCAAAAAGT+TGG - tig0021533:70862-70881 Msa1392850:CDS 30.0%
AGAGTAGACATCAAAAAGTT+GGG - tig0021533:70863-70882 Msa1392850:CDS 30.0%
!!! TCATTTTCTAGGATCAAATC+AGG - tig0021533:70934-70953 Msa1392850:CDS 30.0%
AGTAATGTTCTACTTACACT+AGG - tig0021533:70994-71013 Msa1392850:CDS 30.0%
AATATTATGGTATGTGCAGA+TGG - tig0021533:71150-71169 Msa1392850:intron 30.0%
!! TCAACAATGTTGTTTCCAAA+GGG + tig0021533:71496-71515 None:intergenic 30.0%
!! ATCAACAATGTTGTTTCCAA+AGG + tig0021533:71497-71516 None:intergenic 30.0%
AAAACCAGACAATTATGACA+TGG - tig0021533:71648-71667 Msa1392850:intron 30.0%
ATCCAAAATTAACCAGTTGA+AGG - tig0021533:72187-72206 Msa1392850:CDS 30.0%
! TTCCTTCAACTGGTTAATTT+TGG + tig0021533:72192-72211 None:intergenic 30.0%
!!! TCCTTTTTTGTCCTTTGATA+AGG - tig0021533:72278-72297 Msa1392850:intron 30.0%
TCCTTATCAAAGGACAAAAA+AGG + tig0021533:72282-72301 None:intergenic 30.0%
! TCTTTTCTACAACATGTTCT+GGG - tig0021533:72410-72429 Msa1392850:intron 30.0%
ACAACAGAAGAAAAACACTT+TGG + tig0021533:72464-72483 None:intergenic 30.0%
TCTAGGTTTGATAGAAAATG+TGG - tig0021533:72640-72659 Msa1392850:intron 30.0%
AATAATCACAAAACGACAAG+AGG + tig0021533:72692-72711 None:intergenic 30.0%
!!! TCGAACATGTTGGTTTGTTT+TGG - tig0021533:69634-69653 Msa1392850:three_prime_UTR 35.0%
CGATTTGATTAGATGTGGTA+TGG + tig0021533:69734-69753 None:intergenic 35.0%
AGTAGTATGCTTACAAGTTC+AGG + tig0021533:69810-69829 None:intergenic 35.0%
TCGACCAGAAAAATAAAGCT+TGG + tig0021533:69941-69960 None:intergenic 35.0%
GTTTCAACTGGGAAACAATT+TGG + tig0021533:70142-70161 None:intergenic 35.0%
CATCAAGATGAGTTTCAACT+GGG + tig0021533:70153-70172 None:intergenic 35.0%
TCATCAAGATGAGTTTCAAC+TGG + tig0021533:70154-70173 None:intergenic 35.0%
! CTCATCTTGATGATCAAACA+AGG - tig0021533:70161-70180 Msa1392850:intron 35.0%
TATCGATGTCTTGATCTTCT+TGG + tig0021533:70191-70210 None:intergenic 35.0%
! AATGAGTTGAGGTGATGAAT+AGG + tig0021533:70224-70243 None:intergenic 35.0%
!! CTCGACTTTAGGTTTTACAT+CGG + tig0021533:70308-70327 None:intergenic 35.0%
! TTACACCTTTTCTCGACTTT+AGG + tig0021533:70319-70338 None:intergenic 35.0%
! CAAAGATTTTCTTCCAGCTT+TGG - tig0021533:70345-70364 Msa1392850:intron 35.0%
! CTTTTCCCTTCGTTTTCTTT+CGG + tig0021533:70470-70489 None:intergenic 35.0%
CACCATTTCAGCCTTTAATA+TGG + tig0021533:70641-70660 None:intergenic 35.0%
GAGTAGACATCAAAAAGTTG+GGG - tig0021533:70864-70883 Msa1392850:CDS 35.0%
GCTGAAGACAACAAAAAAAC+TGG - tig0021533:71250-71269 Msa1392850:intron 35.0%
GATACTCTTACTTCGATTTC+AGG + tig0021533:71361-71380 None:intergenic 35.0%
ATTCAATGTGACAAACACAG+TGG - tig0021533:71403-71422 Msa1392850:intron 35.0%
TACTTTGGACAGTGAGATTA+AGG + tig0021533:71462-71481 None:intergenic 35.0%
TGATGTAACCATTGTTGCTA+AGG - tig0021533:71513-71532 Msa1392850:intron 35.0%
TGTCCTTTGATAAGGATTCT+AGG - tig0021533:72286-72305 Msa1392850:intron 35.0%
GTCCTTTGATAAGGATTCTA+GGG - tig0021533:72287-72306 Msa1392850:intron 35.0%
AACCCTAGAATCCTTATCAA+AGG + tig0021533:72292-72311 None:intergenic 35.0%
TAGGGTTACTATATCCATGA+AGG - tig0021533:72305-72324 Msa1392850:intron 35.0%
GTTACTATATCCATGAAGGA+AGG - tig0021533:72309-72328 Msa1392850:intron 35.0%
! GTCTTTTCTACAACATGTTC+TGG - tig0021533:72409-72428 Msa1392850:intron 35.0%
!! TTTGCATAGTTGACTTGACT+AGG - tig0021533:72489-72508 Msa1392850:CDS 35.0%
! TACTTTTGAACAGGGAATGG+AGG - tig0021533:70021-70040 Msa1392850:three_prime_UTR 40.0%
CTTCTTTAACCAATACCCGA+TGG - tig0021533:70051-70070 Msa1392850:exon 40.0%
! AACTGGGAAACAATTTGGTG+TGG + tig0021533:70137-70156 None:intergenic 40.0%
GATGTCTTGATCTTCTTGGT+TGG + tig0021533:70187-70206 None:intergenic 40.0%
GAGGTGATGAATAGGAAGAA+GGG + tig0021533:70216-70235 None:intergenic 40.0%
TGAGGTGATGAATAGGAAGA+AGG + tig0021533:70217-70236 None:intergenic 40.0%
!! AGAAGCAATTGGTGTTGAAC+TGG + tig0021533:70260-70279 None:intergenic 40.0%
ATAACCAAGTGAATCAGACC+AGG - tig0021533:70413-70432 Msa1392850:intron 40.0%
TCAAGCTCGAGAACATGTAA+TGG - tig0021533:70444-70463 Msa1392850:intron 40.0%
TAATGGCCGAAAGAAAACGA+AGG - tig0021533:70461-70480 Msa1392850:intron 40.0%
AATGGCCGAAAGAAAACGAA+GGG - tig0021533:70462-70481 Msa1392850:intron 40.0%
TGAAAGAGCGATGAAACTTC+GGG + tig0021533:70497-70516 None:intergenic 40.0%
CTGAAAGAGCGATGAAACTT+CGG + tig0021533:70498-70517 None:intergenic 40.0%
ATGGACAAAGCTTCTGTGTT+GGG - tig0021533:71169-71188 Msa1392850:intron 40.0%
AGATCTCTTCACTTGAACTG+TGG + tig0021533:71278-71297 None:intergenic 40.0%
! TTCAGGAAGTGGTTGGTTAT+TGG + tig0021533:71344-71363 None:intergenic 40.0%
CTTACTTCGATTTCAGGAAG+TGG + tig0021533:71355-71374 None:intergenic 40.0%
!! AGGGCAAAAAGGTACTACTT+TGG + tig0021533:71477-71496 None:intergenic 40.0%
! AGTAGTACCTTTTTGCCCTT+TGG - tig0021533:71478-71497 Msa1392850:intron 40.0%
! GTTGTTTCCAAAGGGCAAAA+AGG + tig0021533:71488-71507 None:intergenic 40.0%
ACGACCATGTCATAATTGTC+TGG + tig0021533:71655-71674 None:intergenic 40.0%
!!! TTTTTTTTTTGTGGTGGCCG+AGG - tig0021533:71909-71928 Msa1392850:CDS 40.0%
TTAGTGAGATGTTCCAACCT+CGG + tig0021533:71929-71948 None:intergenic 40.0%
TCAGCAACGAACATTAGTGA+CGG + tig0021533:72003-72022 None:intergenic 40.0%
AATTCTCTTGCAGAGGAACA+AGG - tig0021533:72111-72130 Msa1392850:CDS 40.0%
ATCATAGATTCTCCCATCAC+AGG + tig0021533:72336-72355 None:intergenic 40.0%
! TTCTACAACATGTTCTGGGT+CGG - tig0021533:72414-72433 Msa1392850:intron 40.0%
GTGTGTGGAATTTCAACAGA+TGG - tig0021533:72436-72455 Msa1392850:intron 40.0%
CAGGAAAGAAGGAAGAAAAG+AGG + tig0021533:72608-72627 None:intergenic 40.0%
! CCTTTTCACAACAGGAAAGA+AGG + tig0021533:72619-72638 None:intergenic 40.0%
CCTTCTTTCCTGTTGTGAAA+AGG - tig0021533:72616-72635 Msa1392850:intron 40.0%
TCCTGTTGTGAAAAGGTTCT+AGG - tig0021533:72623-72642 Msa1392850:intron 40.0%
! ACCTAGAACCTTTTCACAAC+AGG + tig0021533:72627-72646 None:intergenic 40.0%
ACAAGTTCAGGCACATAGGT+GGG + tig0021533:69798-69817 None:intergenic 45.0%
TACAAGTTCAGGCACATAGG+TGG + tig0021533:69799-69818 None:intergenic 45.0%
GCTTACAAGTTCAGGCACAT+AGG + tig0021533:69802-69821 None:intergenic 45.0%
CAAGAGAATCCATCGGGTAT+TGG + tig0021533:70063-70082 None:intergenic 45.0%
GAGATGCAAGAGAATCCATC+GGG + tig0021533:70069-70088 None:intergenic 45.0%
GGAGATGCAAGAGAATCCAT+CGG + tig0021533:70070-70089 None:intergenic 45.0%
! CTTCCAGCTTTGGTTTCTCA+AGG - tig0021533:70355-70374 Msa1392850:intron 45.0%
GAACCTTGAGAAACCAAAGC+TGG + tig0021533:70361-70380 None:intergenic 45.0%
TGTTCCTGGTCTGATTCACT+TGG + tig0021533:70420-70439 None:intergenic 45.0%
!!! CTTTTTGAGGCCAGGAACAA+TGG + tig0021533:70521-70540 None:intergenic 45.0%
CATTGTTCCTGGCCTCAAAA+AGG - tig0021533:70519-70538 Msa1392850:intron 45.0%
!!! GATCATACCTTTTTGAGGCC+AGG + tig0021533:70529-70548 None:intergenic 45.0%
GATGGACAAAGCTTCTGTGT+TGG - tig0021533:71168-71187 Msa1392850:intron 45.0%
CTTCGATTTCAGGAAGTGGT+TGG + tig0021533:71351-71370 None:intergenic 45.0%
CAGCAACGAACATTAGTGAC+GGG + tig0021533:72002-72021 None:intergenic 45.0%
GGAAAACTGTGTGACAGCAA+AGG - tig0021533:72132-72151 Msa1392850:CDS 45.0%
ATGAAGGAAGGTCCTGTGAT+GGG - tig0021533:72321-72340 Msa1392850:intron 45.0%
!! TTTAATAATGTATTATATAT+AGG - tig0021533:71551-71570 Msa1392850:intron 5.0%
!! AAAAAAAAAAAAAAAAAAAG+AGG + tig0021533:71899-71918 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTTTTTTG+TGG - tig0021533:71900-71919 Msa1392850:CDS 5.0%
AAGTTCAGGCACATAGGTGG+GGG + tig0021533:69796-69815 None:intergenic 50.0%
CAAGTTCAGGCACATAGGTG+GGG + tig0021533:69797-69816 None:intergenic 50.0%
GCTCTTTCAGCCATTGTTCC+TGG - tig0021533:70508-70527 Msa1392850:intron 50.0%
ACAAGCACGTTTGCAGACAC+TGG - tig0021533:71219-71238 Msa1392850:intron 50.0%
! TAATTGGCCAAGCACCGTAG+CGG + tig0021533:71434-71453 None:intergenic 50.0%
!!! TTTTTTGTGGTGGCCGAGGT+TGG - tig0021533:71913-71932 Msa1392850:CDS 50.0%
TGTGACAGCAAAGGATCTCC+TGG - tig0021533:72141-72160 Msa1392850:CDS 50.0%
GTGACAGCAAAGGATCTCCT+GGG - tig0021533:72142-72161 Msa1392850:CDS 50.0%
CATCACAGGACCTTCCTTCA+TGG + tig0021533:72322-72341 None:intergenic 50.0%
CATGAAGGAAGGTCCTGTGA+TGG - tig0021533:72320-72339 Msa1392850:intron 50.0%
TCGAGCTTGAGCAGTGTTCC+TGG + tig0021533:70434-70453 None:intergenic 55.0%
AGCACGTTTGCAGACACTGG+AGG - tig0021533:71222-71241 Msa1392850:intron 55.0%
! TCTCCTGGGAAGCATACGAC+AGG - tig0021533:72156-72175 Msa1392850:CDS 55.0%
AAGCCTGTCGTATGCTTCCC+AGG + tig0021533:72162-72181 None:intergenic 55.0%
! ACATGTTCTGGGTCGGTGTG+TGG - tig0021533:72421-72440 Msa1392850:intron 55.0%
! ACACAGTGGACGTGCCGCTA+CGG - tig0021533:71417-71436 Msa1392850:intron 60.0%
! GGACGTGCCGCTACGGTGCT+TGG - tig0021533:71424-71443 Msa1392850:intron 70.0%
Chromosome Type Strat End Strand Name
tig0021533 gene 69501 72751 69501 ID=Msa1392850;Name=Msa1392850
tig0021533 mRNA 69501 72751 69501 ID=Msa1392850-mRNA-1;Parent=Msa1392850;Name=Msa1392850-mRNA-1;_AED=0.06;_eAED=0.06;_QI=74|1|1|1|0.75|0.6|5|553|345
tig0021533 exon 69501 70128 69501 ID=Msa1392850-mRNA-1:exon:13485;Parent=Msa1392850-mRNA-1
tig0021533 exon 70718 71083 70718 ID=Msa1392850-mRNA-1:exon:13484;Parent=Msa1392850-mRNA-1
tig0021533 exon 71712 72218 71712 ID=Msa1392850-mRNA-1:exon:13483;Parent=Msa1392850-mRNA-1
tig0021533 exon 72437 72529 72437 ID=Msa1392850-mRNA-1:exon:13482;Parent=Msa1392850-mRNA-1
tig0021533 exon 72681 72751 72681 ID=Msa1392850-mRNA-1:exon:13481;Parent=Msa1392850-mRNA-1
tig0021533 five_prime_UTR 72681 72751 72681 ID=Msa1392850-mRNA-1:five_prime_utr;Parent=Msa1392850-mRNA-1
tig0021533 five_prime_UTR 72527 72529 72527 ID=Msa1392850-mRNA-1:five_prime_utr;Parent=Msa1392850-mRNA-1
tig0021533 CDS 72437 72526 72437 ID=Msa1392850-mRNA-1:cds;Parent=Msa1392850-mRNA-1
tig0021533 CDS 71712 72218 71712 ID=Msa1392850-mRNA-1:cds;Parent=Msa1392850-mRNA-1
tig0021533 CDS 70718 71083 70718 ID=Msa1392850-mRNA-1:cds;Parent=Msa1392850-mRNA-1
tig0021533 CDS 70054 70128 70054 ID=Msa1392850-mRNA-1:cds;Parent=Msa1392850-mRNA-1
tig0021533 three_prime_UTR 69501 70053 69501 ID=Msa1392850-mRNA-1:three_prime_utr;Parent=Msa1392850-mRNA-1
Gene Sequence

>Msa1392850

ATGTCCCATACCACATCTAATCAAATCGAAGTTTTAGGAAAGAAACTGAAGATGATGCAAATTATGTCCCCCACCTATGTGCCTGAACTTGGAATGGAGGATCTCAACTTCTTTAACCAATACCCGATGGATTCTCTTGCATCTCCAATATTATTCGACAACTTTGGCTTTCACTTTGATGACAACGAAACCACACCAAATTGTTTCCCAGTTGAAACTCATCTTGATGATCAAACAAGGCCAACCAAGAAGATCAAGACATCGATAAACCCTTCTTCCTATTCATCACCTCAACTCATTTCTTTTGAACACTCCAGTTCAACACCAATTGCTTCTAAGAAATTTTATAACCTTGATTATTCCGATGTAAAACCTAAAGTCGAGAAAAGGTGTAATGATAACAAAGATTTTCTTCCAGCTTTGGTTTCTCAAGGTTCTTATGAAGATCAAAAAATATTTTCAAATTATGATAACCAAGTGAATCAGACCAGGAACACTGCTCAAGCTCGAGAACATGTAATGGCCGAAAGAAAACGAAGGGAAAAGCTTACCCGAAGTTTCATCGCTCTTTCAGCCATTGTTCCTGGCCTCAAAAAGATGGACAAAGCTTCTGTGTTGGGAGATGCTATCAAGTACATGAAACAACTACAAGCACGTTTGCAGACACTGGAGGAACAAGCTGAAGACAACAAAAAAACTGGATCCACAGTTCAAGTGAAGAGATCTATTATCTTCACTAATGATGACGATGATGATGATGATAGCAACTCCAATAACCAACCACTTCCTGAAATCGAAGTAAGAGTATCAAGCAAAGATGTACTCATCAAGATTCAATGTGACAAACACAGTGGACGTGCCGCTACGGTGCTTGGCCAATTAGAAAACCTTAATCTCACTGTCCAAAGTAGTACCTTTTTGCCCTTTGGAAACAACATTGTTGATGTAACCATTGTTGCTAAGAGGAACAAGGAAAACTGTGTGACAGCAAAGGATCTCCTGGGAAGCATACGACAGGCTTTAATCATCCAAAATTAA
Protein sequence

>Msa1392850

MSHTTSNQIEVLGKKLKMMQIMSPTYVPELGMEDLNFFNQYPMDSLASPILFDNFGFHFDDNETTPNCFPVETHLDDQTRPTKKIKTSINPSSYSSPQLISFEHSSSTPIASKKFYNLDYSDVKPKVEKRCNDNKDFLPALVSQGSYEDQKIFSNYDNQVNQTRNTAQAREHVMAERKRREKLTRSFIALSAIVPGLKKMDKASVLGDAIKYMKQLQARLQTLEEQAEDNKKTGSTVQVKRSIIFTNDDDDDDDSNSNNQPLPEIEVRVSSKDVLIKIQCDKHSGRAATVLGQLENLNLTVQSSTFLPFGNNIVDVTIVAKRNKENCVTAKDLLGSIRQALIIQN*