AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0754590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0754590 MtrunA17_Chr5g0400671 94.207 328 17 1 18 345 1 326 0.0 610
Msa0754590 MtrunA17_Chr4g0054561 47.175 354 127 15 18 341 1 324 8.53e-82 251
Msa0754590 MtrunA17_Chr4g0054551 44.350 354 137 15 18 341 1 324 5.41e-76 237
Msa0754590 MtrunA17_Chr2g0280161 35.328 351 191 11 19 340 5 348 7.40e-53 178
Msa0754590 MtrunA17_Chr4g0049331 33.851 322 185 10 32 341 1 306 1.68e-49 168
Msa0754590 MtrunA17_Chr5g0400661 39.916 238 122 6 121 342 102 334 4.48e-46 159
Msa0754590 MtrunA17_Chr5g0400621 38.023 263 143 4 80 340 83 327 5.46e-45 157
Msa0754590 MtrunA17_Chr4g0002781 38.168 262 131 7 91 342 109 349 3.07e-44 155
Msa0754590 MtrunA17_Chr2g0333621 36.047 258 139 6 92 342 73 311 1.58e-42 150
Msa0754590 MtrunA17_Chr4g0002801 39.823 226 117 4 133 342 108 330 2.28e-42 150
Msa0754590 MtrunA17_Chr4g0033391 41.436 181 100 2 164 342 140 316 4.12e-41 146
Msa0754590 MtrunA17_Chr2g0333611 40.000 185 102 4 162 342 36 215 2.51e-40 141
Msa0754590 MtrunA17_Chr8g0336681 39.153 189 103 3 164 342 149 335 1.40e-39 142
Msa0754590 MtrunA17_Chr7g0225901 45.161 155 84 1 168 321 85 239 3.08e-39 139
Msa0754590 MtrunA17_Chr2g0307131 41.489 188 104 2 158 339 161 348 8.74e-39 141
Msa0754590 MtrunA17_Chr4g0033291 41.262 206 104 4 143 342 130 324 2.64e-38 139
Msa0754590 MtrunA17_Chr6g0468491 41.379 174 101 1 166 338 132 305 2.81e-38 138
Msa0754590 MtrunA17_Chr2g0307141 43.429 175 92 3 169 339 181 352 4.47e-38 139
Msa0754590 MtrunA17_Chr4g0054581 40.201 199 103 6 155 342 181 374 1.59e-37 138
Msa0754590 MtrunA17_Chr7g0249811 39.665 179 100 1 164 342 158 328 1.49e-36 134
Msa0754590 MtrunA17_Chr5g0400641 44.079 152 84 1 174 324 1 152 2.09e-36 129
Msa0754590 MtrunA17_Chr2g0333641 34.263 251 139 6 104 341 69 306 5.04e-36 132
Msa0754590 MtrunA17_Chr4g0033281 38.308 201 116 4 143 342 128 321 3.30e-35 130
Msa0754590 MtrunA17_Chr2g0333541 40.860 186 103 3 164 344 87 270 1.06e-32 123
Msa0754590 MtrunA17_Chr4g0054621 35.263 190 114 3 155 342 155 337 1.62e-32 124
Msa0754590 MtrunA17_Chr4g0054571 37.500 192 106 2 162 342 141 329 1.15e-31 121
Msa0754590 MtrunA17_Chr6g0468461 40.385 156 80 1 166 321 168 310 3.28e-30 118
Msa0754590 MtrunA17_Chr4g0002851 35.762 151 80 1 194 344 65 198 1.75e-20 88.2
Msa0754590 MtrunA17_Chr8g0366751 36.196 163 70 5 164 301 451 604 3.99e-20 91.7
Msa0754590 MtrunA17_Chr7g0233441 33.775 151 91 1 194 344 57 198 3.25e-19 84.3
Msa0754590 MtrunA17_Chr4g0002831 31.098 164 102 3 182 344 53 206 1.73e-18 82.8
Msa0754590 MtrunA17_Chr1g0185621 33.333 183 104 5 172 341 476 653 1.90e-18 86.7
Msa0754590 MtrunA17_Chr7g0275821 28.729 181 106 2 172 344 253 418 9.09e-18 84.0
Msa0754590 MtrunA17_Chr5g0411341 34.810 158 87 2 164 305 482 639 1.19e-17 84.3
Msa0754590 MtrunA17_Chr7g0252041 30.481 187 110 4 171 343 286 466 2.38e-16 79.7
Msa0754590 MtrunA17_Chr8g0346161 30.058 173 116 2 172 339 292 464 2.07e-15 77.0
Msa0754590 MtrunA17_Chr8g0346151 36.029 136 71 4 172 295 280 411 2.78e-15 76.6
Msa0754590 MtrunA17_Chr8g0365711 25.146 171 122 2 155 319 228 398 9.28e-15 74.7
Msa0754590 MtrunA17_Chr7g0260841 34.146 123 54 3 162 280 342 441 4.91e-13 69.7
Msa0754590 MtrunA17_Chr3g0131691 29.560 159 97 4 171 316 298 454 1.06e-12 68.9
Msa0754590 MtrunA17_Chr1g0211081 32.090 134 82 3 103 235 256 381 1.61e-12 68.2
Msa0754590 MtrunA17_Chr5g0411541 26.596 282 170 9 85 342 47 315 1.95e-12 67.4
Msa0754590 MtrunA17_Chr4g0028071 31.206 141 82 4 173 304 151 285 2.10e-12 67.4
Msa0754590 MtrunA17_Chr8g0386921 28.313 166 83 4 172 304 407 569 3.39e-12 67.4
Msa0754590 MtrunA17_Chr2g0320291 30.714 140 84 4 173 306 135 267 2.26e-11 63.9
Msa0754590 MtrunA17_Chr3g0141091 29.379 177 106 4 167 342 169 327 2.77e-11 63.9
Msa0754590 MtrunA17_Chr2g0298751 24.352 193 130 5 149 326 29 220 3.34e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0754590 AT4G37850.2 35.988 339 186 13 19 341 1 324 4.18e-49 167
Msa0754590 AT4G37850.1 35.988 339 186 13 19 341 1 324 4.18e-49 167
Msa0754590 AT2G22760.1 43.784 185 99 2 163 342 111 295 8.85e-46 158
Msa0754590 AT2G22760.2 46.012 163 84 1 163 321 111 273 2.37e-43 151
Msa0754590 AT2G22750.5 33.819 343 153 14 19 323 1 307 3.44e-42 149
Msa0754590 AT2G22750.3 33.819 343 153 14 19 323 5 311 3.54e-42 149
Msa0754590 AT2G22750.4 33.819 343 153 14 19 323 5 311 1.09e-41 148
Msa0754590 AT2G22750.2 45.763 177 69 4 163 323 118 283 1.69e-39 142
Msa0754590 AT2G22750.1 45.763 177 69 4 163 323 118 283 4.07e-39 140
Msa0754590 AT2G22770.1 39.810 211 109 3 149 341 108 318 5.92e-37 135
Msa0754590 AT4G17880.1 29.675 246 127 8 90 306 324 552 4.55e-20 91.7
Msa0754590 AT5G46760.1 34.783 161 78 4 164 306 404 555 4.98e-19 88.6
Msa0754590 AT1G32640.1 35.099 151 92 3 162 306 439 589 1.01e-16 81.6
Msa0754590 AT2G46510.1 26.667 180 111 3 162 337 382 544 3.38e-16 80.1
Msa0754590 AT1G01260.1 35.099 151 64 4 167 307 429 555 4.92e-16 79.7
Msa0754590 AT1G01260.2 35.099 151 64 4 167 307 429 555 4.92e-16 79.7
Msa0754590 AT1G01260.3 35.099 151 64 4 167 307 429 555 4.92e-16 79.7
Msa0754590 AT5G57150.4 26.455 189 119 3 149 322 33 216 1.66e-15 75.9
Msa0754590 AT5G57150.5 26.344 186 117 3 149 319 32 212 2.25e-15 74.7
Msa0754590 AT5G57150.3 26.344 186 117 3 149 319 33 213 3.09e-15 74.3
Msa0754590 AT5G57150.2 26.344 186 117 3 149 319 33 213 3.13e-15 74.3
Msa0754590 AT5G57150.1 26.154 195 124 3 149 328 32 221 3.18e-15 74.7
Msa0754590 AT1G12860.1 29.444 180 115 4 171 343 267 441 1.05e-14 75.1
Msa0754590 AT1G12860.2 31.373 153 98 3 171 316 267 419 1.27e-14 74.7
Msa0754590 AT4G09820.1 34.884 129 63 2 172 299 364 472 1.98e-14 74.7
Msa0754590 AT2G16910.1 27.684 177 105 4 172 325 315 491 8.41e-14 72.8
Msa0754590 AT1G63650.3 29.082 196 101 7 172 341 406 589 1.63e-13 72.0
Msa0754590 AT1G63650.1 29.082 196 101 7 172 341 406 589 1.63e-13 72.0
Msa0754590 AT1G63650.2 29.082 196 101 7 172 341 406 589 1.63e-13 72.0
Msa0754590 AT4G29930.5 30.345 145 88 3 172 313 55 189 2.67e-13 68.9
Msa0754590 AT4G29930.2 30.345 145 88 3 172 313 55 189 3.64e-13 68.6
Msa0754590 AT4G29930.6 30.822 146 89 3 172 313 55 192 3.73e-13 68.6
Msa0754590 AT3G26744.1 29.143 175 108 4 171 334 307 476 3.42e-12 67.8
Msa0754590 AT3G26744.4 29.143 175 108 4 171 334 307 476 3.42e-12 67.8
Msa0754590 AT3G26744.2 29.143 175 108 4 171 334 307 476 3.42e-12 67.8
Msa0754590 AT5G65640.1 28.889 180 112 5 167 342 174 341 3.77e-12 67.0
Msa0754590 AT3G26744.6 30.573 157 98 3 171 316 307 463 5.86e-12 67.0
Msa0754590 AT4G16430.1 45.333 75 37 1 162 232 307 381 6.49e-12 66.6
Msa0754590 AT4G00870.1 29.927 137 92 1 172 304 250 386 6.88e-12 66.6
Msa0754590 AT4G29930.3 26.829 164 98 3 172 313 55 218 1.79e-11 64.3
Msa0754590 AT4G29930.7 26.829 164 98 3 172 313 55 218 1.79e-11 64.3
Msa0754590 AT5G41315.3 28.788 198 104 8 172 341 447 635 2.29e-11 65.5
Msa0754590 AT5G41315.2 28.788 198 104 8 172 341 450 638 2.34e-11 65.5
Msa0754590 AT5G41315.1 28.788 198 104 8 172 341 442 630 2.43e-11 65.5
Msa0754590 AT4G29930.1 26.829 164 98 3 172 313 55 218 2.56e-11 63.5

Find 91 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTCAACTGGTTAATTT+TGG 0.110602 5_2:+8180355 None:intergenic
TTTCTCATCTTACACCAATT+TGG 0.189336 5_2:-8183054 None:intergenic
GATACTCTTACTTCGATTTC+AGG 0.236620 5_2:+8181184 None:intergenic
GTTTCAACTGGGAAACAATT+TGG 0.250112 5_2:+8182403 None:intergenic
TACTTTGGACAGTGAGATTA+AGG 0.260165 5_2:+8181083 None:intergenic
TTCAGGAAGTGGTTGGTTAT+TGG 0.276191 5_2:+8181201 None:intergenic
TCCTGTTGTGAAAAGGTTCT+AGG 0.284232 5_2:-8179921 Msa0754590:three_prime_UTR
TATCGATGTCTTGATCTTCT+TGG 0.286833 5_2:+8182354 None:intergenic
GCTCTTTCAGCCATTGTTCC+TGG 0.288584 5_2:-8182034 Msa0754590:CDS
CCAAAGTTGTCGAATAATAT+TGG 0.304765 5_2:+8182454 None:intergenic
TGTCCTTTGATAAGGATTCT+AGG 0.308395 5_2:-8180258 Msa0754590:three_prime_UTR
TCGAGCTTGAGCAGTGTTCC+TGG 0.329696 5_2:+8182111 None:intergenic
AGAGAGTTAATATTCCAAAT+TGG 0.339623 5_2:+8183040 None:intergenic
CCTTCTTTCCTGTTGTGAAA+AGG 0.358968 5_2:-8179928 Msa0754590:three_prime_UTR
GATTAAAATATTCCTTCAAC+TGG 0.373209 5_2:+8180345 None:intergenic
CATCACAGGACCTTCCTTCA+TGG 0.375414 5_2:+8180225 None:intergenic
CGATTTGATTAGATGTGGTA+TGG 0.375743 5_2:+8182811 None:intergenic
TAAAATTTCTTAGAAGCAAT+TGG 0.377682 5_2:+8182274 None:intergenic
CATTGTTCCTGGCCTCAAAA+AGG 0.384959 5_2:-8182023 Msa0754590:intron
TCATCAAGATGAGTTTCAAC+TGG 0.389714 5_2:+8182391 None:intergenic
CTTCCAGCTTTGGTTTCTCA+AGG 0.393979 5_2:-8182187 Msa0754590:CDS
AATGAGTTGAGGTGATGAAT+AGG 0.399012 5_2:+8182321 None:intergenic
GTTGTTTCCAAAGGGCAAAA+AGG 0.400410 5_2:+8181057 None:intergenic
CCAATATTATTCGACAACTT+TGG 0.407380 5_2:-8182454 Msa0754590:CDS
AGTAGTATGCTTACAAGTTC+AGG 0.414356 5_2:+8182735 None:intergenic
AGAAGCAATTGGTGTTGAAC+TGG 0.417121 5_2:+8182285 None:intergenic
TGAGGTGATGAATAGGAAGA+AGG 0.434612 5_2:+8182328 None:intergenic
ATGGACAAAGCTTCTGTGTT+GGG 0.439391 5_2:-8181373 Msa0754590:CDS
AAATAAATTAGAAGAGAAAG+TGG 0.442399 5_2:+8183010 None:intergenic
TGTGACAGCAAAGGATCTCC+TGG 0.446189 5_2:-8180403 Msa0754590:CDS
CAAGAGAATCCATCGGGTAT+TGG 0.448559 5_2:+8182482 None:intergenic
GTCCTTTGATAAGGATTCTA+GGG 0.456194 5_2:-8180257 Msa0754590:three_prime_UTR
TGAAAGAGCGATGAAACTTC+GGG 0.456547 5_2:+8182048 None:intergenic
ATCCAAAATTAACCAGTTGA+AGG 0.460869 5_2:-8180357 Msa0754590:exon
GAGGTGATGAATAGGAAGAA+GGG 0.466954 5_2:+8182329 None:intergenic
GGACGTGCCGCTACGGTGCT+TGG 0.467945 5_2:-8181118 Msa0754590:CDS
ATCAACAATGTTGTTTCCAA+AGG 0.469768 5_2:+8181048 None:intergenic
TCAACAATGTTGTTTCCAAA+GGG 0.475298 5_2:+8181049 None:intergenic
GATGTCTTGATCTTCTTGGT+TGG 0.487867 5_2:+8182358 None:intergenic
CTGAAAGAGCGATGAAACTT+CGG 0.488970 5_2:+8182047 None:intergenic
AAGCCTGTCGTATGCTTCCC+AGG 0.497417 5_2:+8180385 None:intergenic
TTCTACAACATGTTCTGGGT+CGG 0.501517 5_2:-8180130 Msa0754590:three_prime_UTR
ATTAACAATTACACATGTGA+TGG 0.502857 5_2:+8180017 None:intergenic
CTTCGATTTCAGGAAGTGGT+TGG 0.508259 5_2:+8181194 None:intergenic
TAAAACCTAAAGTCGAGAAA+AGG 0.508413 5_2:-8182231 Msa0754590:CDS
TGTTCCTGGTCTGATTCACT+TGG 0.510038 5_2:+8182125 None:intergenic
CTTACTTCGATTTCAGGAAG+TGG 0.529255 5_2:+8181190 None:intergenic
TTTATTTACCTTAGCAACAA+TGG 0.529541 5_2:+8181021 None:intergenic
GATTTGATTAGATGTGGTAT+GGG 0.531705 5_2:+8182812 None:intergenic
GAACCTTGAGAAACCAAAGC+TGG 0.540922 5_2:+8182184 None:intergenic
TGATGTAACCATTGTTGCTA+AGG 0.545162 5_2:-8181029 Msa0754590:intron
ACAAGTTCAGGCACATAGGT+GGG 0.548457 5_2:+8182747 None:intergenic
ATCATAGATTCTCCCATCAC+AGG 0.549532 5_2:+8180211 None:intergenic
ACAAGCACGTTTGCAGACAC+TGG 0.553923 5_2:-8181323 Msa0754590:CDS
TCTAGGTTTGATAGAAAATG+TGG 0.554779 5_2:-8179904 Msa0754590:three_prime_UTR
AATAATCACAAAACGACAAG+AGG 0.560394 5_2:+8179855 None:intergenic
AACTTCGATTTGATTAGATG+TGG 0.560569 5_2:+8182806 None:intergenic
ATGAAGGAAGGTCCTGTGAT+GGG 0.560852 5_2:-8180223 Msa0754590:three_prime_UTR
ACACAGTGGACGTGCCGCTA+CGG 0.562208 5_2:-8181125 Msa0754590:CDS
AATTCTCTTGCAGAGGAACA+AGG 0.562972 5_2:-8180433 Msa0754590:intron
AACCCTAGAATCCTTATCAA+AGG 0.573151 5_2:+8180255 None:intergenic
CAAGTTCAGGCACATAGGTG+GGG 0.574321 5_2:+8182748 None:intergenic
CTTCTTTAACCAATACCCGA+TGG 0.574424 5_2:-8182491 Msa0754590:CDS
TTTGCATAGTTGACTTGACT+AGG 0.577273 5_2:-8180055 Msa0754590:three_prime_UTR
ACATGTTCTGGGTCGGTGTG+TGG 0.580330 5_2:-8180123 Msa0754590:three_prime_UTR
CATGAAGGAAGGTCCTGTGA+TGG 0.581540 5_2:-8180224 Msa0754590:three_prime_UTR
CAGGAAAGAAGGAAGAAAAG+AGG 0.581719 5_2:+8179939 None:intergenic
AACTGGGAAACAATTTGGTG+TGG 0.582222 5_2:+8182408 None:intergenic
TAGGGTTACTATATCCATGA+AGG 0.583367 5_2:-8180239 Msa0754590:three_prime_UTR
TCAAGCTCGAGAACATGTAA+TGG 0.585662 5_2:-8182098 Msa0754590:CDS
TACAAGTTCAGGCACATAGG+TGG 0.586428 5_2:+8182746 None:intergenic
GAGATGCAAGAGAATCCATC+GGG 0.590902 5_2:+8182476 None:intergenic
GCTTACAAGTTCAGGCACAT+AGG 0.592149 5_2:+8182743 None:intergenic
CTCATCTTGATGATCAAACA+AGG 0.597867 5_2:-8182381 Msa0754590:CDS
CATCAAGATGAGTTTCAACT+GGG 0.598938 5_2:+8182392 None:intergenic
GTGTGTGGAATTTCAACAGA+TGG 0.598947 5_2:-8180108 Msa0754590:three_prime_UTR
TCTCCTGGGAAGCATACGAC+AGG 0.600421 5_2:-8180388 Msa0754590:CDS
GATGGACAAAGCTTCTGTGT+TGG 0.607222 5_2:-8181374 Msa0754590:CDS
GTGACAGCAAAGGATCTCCT+GGG 0.607386 5_2:-8180402 Msa0754590:CDS
AGATCTCTTCACTTGAACTG+TGG 0.609751 5_2:+8181267 None:intergenic
GTTACTATATCCATGAAGGA+AGG 0.623815 5_2:-8180235 Msa0754590:three_prime_UTR
GGAAAACTGTGTGACAGCAA+AGG 0.624039 5_2:-8180412 Msa0754590:CDS
GGAGATGCAAGAGAATCCAT+CGG 0.627162 5_2:+8182475 None:intergenic
TGTTCAAAAGAAATGAGTTG+AGG 0.643051 5_2:+8182310 None:intergenic
AAGTTCAGGCACATAGGTGG+GGG 0.650925 5_2:+8182749 None:intergenic
AATGGCCGAAAGAAAACGAA+GGG 0.657033 5_2:-8182080 Msa0754590:CDS
TAATTGGCCAAGCACCGTAG+CGG 0.678187 5_2:+8181111 None:intergenic
AGCACGTTTGCAGACACTGG+AGG 0.686426 5_2:-8181320 Msa0754590:CDS
ATAACCAAGTGAATCAGACC+AGG 0.687464 5_2:-8182129 Msa0754590:CDS
TAATGGCCGAAAGAAAACGA+AGG 0.695210 5_2:-8182081 Msa0754590:CDS
ATTCAATGTGACAAACACAG+TGG 0.762327 5_2:-8181139 Msa0754590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATAATTTATTTTTCTTT+TGG - chr5_2:8181033-8181052 Msa0754590:CDS 10.0%
!! AATTAATGTATTCAATATTA+TGG - chr5_2:8181453-8181472 Msa0754590:intron 10.0%
!! AAAATAAATTAACATTTCTA+AGG - chr5_2:8181921-8181940 Msa0754590:intron 10.0%
!!! AAAAAATCATAAATTTTAGA+CGG - chr5_2:8182164-8182183 Msa0754590:CDS 10.0%
!! ACAAAAAAAAAAAAAAAAAG+AGG + chr5_2:8179928-8179947 None:intergenic 10.0%
!!! TCTTTTTTTTTTTTTTTTTG+TGG - chr5_2:8179927-8179946 Msa0754590:three_prime_UTR 10.0%
!!! TAATTTTTCTAATCATTATT+TGG - chr5_2:8182860-8182879 Msa0754590:intron 10.0%
!!! AAGTTTATGAATTTTAAGAT+AGG + chr5_2:8180156-8180175 None:intergenic 15.0%
!!! TTGTTTTTATTTCATTTTCT+AGG - chr5_2:8181239-8181258 Msa0754590:CDS 15.0%
!! TACAATATAAATATTGCAAT+TGG + chr5_2:8181416-8181435 None:intergenic 15.0%
!! TATAGGAAAATATTACATAT+AGG - chr5_2:8181884-8181903 Msa0754590:intron 15.0%
!!! AAATTTTGAAATGAAAAATG+TGG - chr5_2:8182187-8182206 Msa0754590:CDS 15.0%
!!! TTTTTTTTTTTTTTTTGTGG+TGG - chr5_2:8182217-8182236 Msa0754590:CDS 15.0%
!! TTTATGAATTATGATAATCT+AGG + chr5_2:8182401-8182420 None:intergenic 15.0%
!!! TTATGATTCATTTATTTTCA+AGG - chr5_2:8182696-8182715 Msa0754590:intron 15.0%
!!! TATGATTCATTTATTTTCAA+GGG - chr5_2:8182697-8182716 Msa0754590:intron 15.0%
!! TACAATATTAAAAAAAGAAG+TGG + chr5_2:8182983-8183002 None:intergenic 15.0%
!! AAATAAATTAGAAGAGAAAG+TGG + chr5_2:8179851-8179870 None:intergenic 20.0%
!!! TTTATTTTCTTCAATCTTCA+AGG - chr5_2:8179864-8179883 Msa0754590:three_prime_UTR 20.0%
!! AAGCAGATTAAGAATTAAAT+AGG + chr5_2:8179893-8179912 None:intergenic 20.0%
!!! TTAATCTGCTTTTTCTTTTA+GGG - chr5_2:8179902-8179921 Msa0754590:three_prime_UTR 20.0%
!!! TCTTTAATTACTTTTGAACA+GGG - chr5_2:8180329-8180348 Msa0754590:three_prime_UTR 20.0%
!! TAAAATTTCTTAGAAGCAAT+TGG + chr5_2:8180587-8180606 None:intergenic 20.0%
!! ACATTAATGATCCATATTAA+AGG - chr5_2:8180943-8180962 Msa0754590:intron 20.0%
!!! TATTTTCTTATGATCAGATT+AGG + chr5_2:8181102-8181121 None:intergenic 20.0%
!!! TTTTCTAATCATTATTTGGA+TGG - chr5_2:8182864-8182883 Msa0754590:intron 20.0%
! AGAGAGTTAATATTCCAAAT+TGG + chr5_2:8179821-8179840 None:intergenic 25.0%
!!! CTTAATCTGCTTTTTCTTTT+AGG - chr5_2:8179901-8179920 Msa0754590:three_prime_UTR 25.0%
! GTTGAAATCTTAGAATTGAA+GGG + chr5_2:8179983-8180002 None:intergenic 25.0%
! AGTTGAAATCTTAGAATTGA+AGG + chr5_2:8179984-8180003 None:intergenic 25.0%
!! TCTAATCAAATCGAAGTTTT+AGG - chr5_2:8180057-8180076 Msa0754590:three_prime_UTR 25.0%
!!! CTCTTTAATTACTTTTGAAC+AGG - chr5_2:8180328-8180347 Msa0754590:three_prime_UTR 25.0%
! ATTATAACATGTTTGTGAGT+TGG + chr5_2:8180874-8180893 None:intergenic 25.0%
! TGATATAGAAATGGTTTGAA+TGG + chr5_2:8180903-8180922 None:intergenic 25.0%
! GTAGTATGTTGATATAGAAA+TGG + chr5_2:8180912-8180931 None:intergenic 25.0%
! TATTGACGAAAAACATGAAA+TGG + chr5_2:8181019-8181038 None:intergenic 25.0%
!! TTAGTCTTCGAGAAAAAAAT+AGG - chr5_2:8181132-8181151 Msa0754590:CDS 25.0%
! TTGGTAAGTTCAACTTAATT+AGG + chr5_2:8181397-8181416 None:intergenic 25.0%
!! TGAGATTAAGGTTTTCTAAT+TGG + chr5_2:8181766-8181785 None:intergenic 25.0%
! TTTATTTACCTTAGCAACAA+TGG + chr5_2:8181840-8181859 None:intergenic 25.0%
!!! TAACATTTCTAAGGTTGTTT+AGG - chr5_2:8181930-8181949 Msa0754590:intron 25.0%
! GCATACTTGAACATAATAAA+CGG - chr5_2:8182082-8182101 Msa0754590:CDS 25.0%
! AAATATTAATTCTCTTGCAG+AGG - chr5_2:8182418-8182437 Msa0754590:CDS 25.0%
! GATTAAAATATTCCTTCAAC+TGG + chr5_2:8182516-8182535 None:intergenic 25.0%
! ATTAACAATTACACATGTGA+TGG + chr5_2:8182844-8182863 None:intergenic 25.0%
!!! TTTTTTGTCTTCGAACATGT+TGG - chr5_2:8179940-8179959 Msa0754590:three_prime_UTR 30.0%
GATTTGATTAGATGTGGTAT+GGG + chr5_2:8180049-8180068 None:intergenic 30.0%
! AACTTCGATTTGATTAGATG+TGG + chr5_2:8180055-8180074 None:intergenic 30.0%
!!! TTGACCAAGCTTTATTTTTC+TGG - chr5_2:8180250-8180269 Msa0754590:three_prime_UTR 30.0%
!! TTATTTTTCTGGTCGAACTT+TGG - chr5_2:8180261-8180280 Msa0754590:three_prime_UTR 30.0%
!! AATTACTTTTGAACAGGGAA+TGG - chr5_2:8180334-8180353 Msa0754590:three_prime_UTR 30.0%
CCAAAGTTGTCGAATAATAT+TGG + chr5_2:8180407-8180426 None:intergenic 30.0%
CCAATATTATTCGACAACTT+TGG - chr5_2:8180404-8180423 Msa0754590:CDS 30.0%
TGTTCAAAAGAAATGAGTTG+AGG + chr5_2:8180551-8180570 None:intergenic 30.0%
! GTTTTACATCGGAATAATCA+AGG + chr5_2:8180613-8180632 None:intergenic 30.0%
TAAAACCTAAAGTCGAGAAA+AGG - chr5_2:8180627-8180646 Msa0754590:intron 30.0%
! GATAAGATCATACCTTTTTG+AGG + chr5_2:8180850-8180869 None:intergenic 30.0%
ATCCATATTAAAGGCTGAAA+TGG - chr5_2:8180952-8180971 Msa0754590:intron 30.0%
AATGGTCACTAATATTGCTA+GGG + chr5_2:8181001-8181020 None:intergenic 30.0%
AAATGGTCACTAATATTGCT+AGG + chr5_2:8181002-8181021 None:intergenic 30.0%
AAGAGTAGACATCAAAAAGT+TGG - chr5_2:8181178-8181197 Msa0754590:CDS 30.0%
AGAGTAGACATCAAAAAGTT+GGG - chr5_2:8181179-8181198 Msa0754590:CDS 30.0%
!!! TCATTTTCTAGGATCAAATC+AGG - chr5_2:8181250-8181269 Msa0754590:CDS 30.0%
AGTAATGTTCTACTTACACT+AGG - chr5_2:8181310-8181329 Msa0754590:CDS 30.0%
AATATTATGGTATGTGCAGA+TGG - chr5_2:8181466-8181485 Msa0754590:intron 30.0%
!! TCAACAATGTTGTTTCCAAA+GGG + chr5_2:8181812-8181831 None:intergenic 30.0%
!! ATCAACAATGTTGTTTCCAA+AGG + chr5_2:8181813-8181832 None:intergenic 30.0%
AAAACCAGACAATTATGACA+TGG - chr5_2:8181964-8181983 Msa0754590:intron 30.0%
ATCCAAAATTAACCAGTTGA+AGG - chr5_2:8182501-8182520 Msa0754590:CDS 30.0%
! TTCCTTCAACTGGTTAATTT+TGG + chr5_2:8182506-8182525 None:intergenic 30.0%
!!! TCCTTTTTTGTCCTTTGATA+AGG - chr5_2:8182592-8182611 Msa0754590:intron 30.0%
TCCTTATCAAAGGACAAAAA+AGG + chr5_2:8182596-8182615 None:intergenic 30.0%
! TCTTTTCTACAACATGTTCT+GGG - chr5_2:8182724-8182743 Msa0754590:intron 30.0%
ACAACAGAAGAAAAACACTT+TGG + chr5_2:8182778-8182797 None:intergenic 30.0%
TCTAGGTTTGATAGAAAATG+TGG - chr5_2:8182954-8182973 Msa0754590:intron 30.0%
AATAATCACAAAACGACAAG+AGG + chr5_2:8183006-8183025 None:intergenic 30.0%
!!! TCGAACATGTTGGTTTGTTT+TGG - chr5_2:8179950-8179969 Msa0754590:three_prime_UTR 35.0%
CGATTTGATTAGATGTGGTA+TGG + chr5_2:8180050-8180069 None:intergenic 35.0%
AGTAGTATGCTTACAAGTTC+AGG + chr5_2:8180126-8180145 None:intergenic 35.0%
TCGACCAGAAAAATAAAGCT+TGG + chr5_2:8180257-8180276 None:intergenic 35.0%
GTTTCAACTGGGAAACAATT+TGG + chr5_2:8180458-8180477 None:intergenic 35.0%
CATCAAGATGAGTTTCAACT+GGG + chr5_2:8180469-8180488 None:intergenic 35.0%
TCATCAAGATGAGTTTCAAC+TGG + chr5_2:8180470-8180489 None:intergenic 35.0%
! CTCATCTTGATGATCAAACA+AGG - chr5_2:8180477-8180496 Msa0754590:intron 35.0%
TATCGATGTCTTGATCTTCT+TGG + chr5_2:8180507-8180526 None:intergenic 35.0%
! AATGAGTTGAGGTGATGAAT+AGG + chr5_2:8180540-8180559 None:intergenic 35.0%
!! CTCGACTTTAGGTTTTACAT+CGG + chr5_2:8180624-8180643 None:intergenic 35.0%
! TTACACCTTTTCTCGACTTT+AGG + chr5_2:8180635-8180654 None:intergenic 35.0%
! CAAAGATTTTCTTCCAGCTT+TGG - chr5_2:8180661-8180680 Msa0754590:intron 35.0%
! CTTTTCCCTTCGTTTTCTTT+CGG + chr5_2:8180786-8180805 None:intergenic 35.0%
CACCATTTCAGCCTTTAATA+TGG + chr5_2:8180957-8180976 None:intergenic 35.0%
GAGTAGACATCAAAAAGTTG+GGG - chr5_2:8181180-8181199 Msa0754590:CDS 35.0%
GCTGAAGACAACAAAAAAAC+TGG - chr5_2:8181566-8181585 Msa0754590:intron 35.0%
GATACTCTTACTTCGATTTC+AGG + chr5_2:8181677-8181696 None:intergenic 35.0%
ATTCAATGTGACAAACACAG+TGG - chr5_2:8181719-8181738 Msa0754590:intron 35.0%
TACTTTGGACAGTGAGATTA+AGG + chr5_2:8181778-8181797 None:intergenic 35.0%
TGATGTAACCATTGTTGCTA+AGG - chr5_2:8181829-8181848 Msa0754590:intron 35.0%
TGTCCTTTGATAAGGATTCT+AGG - chr5_2:8182600-8182619 Msa0754590:intron 35.0%
GTCCTTTGATAAGGATTCTA+GGG - chr5_2:8182601-8182620 Msa0754590:intron 35.0%
AACCCTAGAATCCTTATCAA+AGG + chr5_2:8182606-8182625 None:intergenic 35.0%
TAGGGTTACTATATCCATGA+AGG - chr5_2:8182619-8182638 Msa0754590:intron 35.0%
GTTACTATATCCATGAAGGA+AGG - chr5_2:8182623-8182642 Msa0754590:intron 35.0%
! GTCTTTTCTACAACATGTTC+TGG - chr5_2:8182723-8182742 Msa0754590:intron 35.0%
!! TTTGCATAGTTGACTTGACT+AGG - chr5_2:8182803-8182822 Msa0754590:CDS 35.0%
! TACTTTTGAACAGGGAATGG+AGG - chr5_2:8180337-8180356 Msa0754590:three_prime_UTR 40.0%
CTTCTTTAACCAATACCCGA+TGG - chr5_2:8180367-8180386 Msa0754590:exon 40.0%
! AACTGGGAAACAATTTGGTG+TGG + chr5_2:8180453-8180472 None:intergenic 40.0%
GATGTCTTGATCTTCTTGGT+TGG + chr5_2:8180503-8180522 None:intergenic 40.0%
GAGGTGATGAATAGGAAGAA+GGG + chr5_2:8180532-8180551 None:intergenic 40.0%
TGAGGTGATGAATAGGAAGA+AGG + chr5_2:8180533-8180552 None:intergenic 40.0%
!! AGAAGCAATTGGTGTTGAAC+TGG + chr5_2:8180576-8180595 None:intergenic 40.0%
ATAACCAAGTGAATCAGACC+AGG - chr5_2:8180729-8180748 Msa0754590:intron 40.0%
TCAAGCTCGAGAACATGTAA+TGG - chr5_2:8180760-8180779 Msa0754590:intron 40.0%
TAATGGCCGAAAGAAAACGA+AGG - chr5_2:8180777-8180796 Msa0754590:intron 40.0%
AATGGCCGAAAGAAAACGAA+GGG - chr5_2:8180778-8180797 Msa0754590:intron 40.0%
TGAAAGAGCGATGAAACTTC+GGG + chr5_2:8180813-8180832 None:intergenic 40.0%
CTGAAAGAGCGATGAAACTT+CGG + chr5_2:8180814-8180833 None:intergenic 40.0%
ATGGACAAAGCTTCTGTGTT+GGG - chr5_2:8181485-8181504 Msa0754590:intron 40.0%
AGATCTCTTCACTTGAACTG+TGG + chr5_2:8181594-8181613 None:intergenic 40.0%
! TTCAGGAAGTGGTTGGTTAT+TGG + chr5_2:8181660-8181679 None:intergenic 40.0%
CTTACTTCGATTTCAGGAAG+TGG + chr5_2:8181671-8181690 None:intergenic 40.0%
!! AGGGCAAAAAGGTACTACTT+TGG + chr5_2:8181793-8181812 None:intergenic 40.0%
! AGTAGTACCTTTTTGCCCTT+TGG - chr5_2:8181794-8181813 Msa0754590:intron 40.0%
! GTTGTTTCCAAAGGGCAAAA+AGG + chr5_2:8181804-8181823 None:intergenic 40.0%
ACGACCATGTCATAATTGTC+TGG + chr5_2:8181971-8181990 None:intergenic 40.0%
!!! TTTTTTTTTTGTGGTGGCCG+AGG - chr5_2:8182223-8182242 Msa0754590:CDS 40.0%
TTAGTGAGATGTTCCAACCT+CGG + chr5_2:8182243-8182262 None:intergenic 40.0%
TCAGCAACGAACATTAGTGA+CGG + chr5_2:8182317-8182336 None:intergenic 40.0%
AATTCTCTTGCAGAGGAACA+AGG - chr5_2:8182425-8182444 Msa0754590:CDS 40.0%
ATCATAGATTCTCCCATCAC+AGG + chr5_2:8182650-8182669 None:intergenic 40.0%
! TTCTACAACATGTTCTGGGT+CGG - chr5_2:8182728-8182747 Msa0754590:intron 40.0%
GTGTGTGGAATTTCAACAGA+TGG - chr5_2:8182750-8182769 Msa0754590:CDS 40.0%
CAGGAAAGAAGGAAGAAAAG+AGG + chr5_2:8182922-8182941 None:intergenic 40.0%
! CCTTTTCACAACAGGAAAGA+AGG + chr5_2:8182933-8182952 None:intergenic 40.0%
CCTTCTTTCCTGTTGTGAAA+AGG - chr5_2:8182930-8182949 Msa0754590:intron 40.0%
TCCTGTTGTGAAAAGGTTCT+AGG - chr5_2:8182937-8182956 Msa0754590:intron 40.0%
! ACCTAGAACCTTTTCACAAC+AGG + chr5_2:8182941-8182960 None:intergenic 40.0%
ACAAGTTCAGGCACATAGGT+GGG + chr5_2:8180114-8180133 None:intergenic 45.0%
TACAAGTTCAGGCACATAGG+TGG + chr5_2:8180115-8180134 None:intergenic 45.0%
GCTTACAAGTTCAGGCACAT+AGG + chr5_2:8180118-8180137 None:intergenic 45.0%
CAAGAGAATCCATCGGGTAT+TGG + chr5_2:8180379-8180398 None:intergenic 45.0%
GAGATGCAAGAGAATCCATC+GGG + chr5_2:8180385-8180404 None:intergenic 45.0%
GGAGATGCAAGAGAATCCAT+CGG + chr5_2:8180386-8180405 None:intergenic 45.0%
! CTTCCAGCTTTGGTTTCTCA+AGG - chr5_2:8180671-8180690 Msa0754590:intron 45.0%
GAACCTTGAGAAACCAAAGC+TGG + chr5_2:8180677-8180696 None:intergenic 45.0%
TGTTCCTGGTCTGATTCACT+TGG + chr5_2:8180736-8180755 None:intergenic 45.0%
!!! CTTTTTGAGGCCAGGAACAA+TGG + chr5_2:8180837-8180856 None:intergenic 45.0%
CATTGTTCCTGGCCTCAAAA+AGG - chr5_2:8180835-8180854 Msa0754590:intron 45.0%
!!! GATCATACCTTTTTGAGGCC+AGG + chr5_2:8180845-8180864 None:intergenic 45.0%
GATGGACAAAGCTTCTGTGT+TGG - chr5_2:8181484-8181503 Msa0754590:intron 45.0%
CTTCGATTTCAGGAAGTGGT+TGG + chr5_2:8181667-8181686 None:intergenic 45.0%
CAGCAACGAACATTAGTGAC+GGG + chr5_2:8182316-8182335 None:intergenic 45.0%
GGAAAACTGTGTGACAGCAA+AGG - chr5_2:8182446-8182465 Msa0754590:CDS 45.0%
ATGAAGGAAGGTCCTGTGAT+GGG - chr5_2:8182635-8182654 Msa0754590:intron 45.0%
!! TTTAATAATGTATTATATAT+AGG - chr5_2:8181867-8181886 Msa0754590:intron 5.0%
AAGTTCAGGCACATAGGTGG+GGG + chr5_2:8180112-8180131 None:intergenic 50.0%
CAAGTTCAGGCACATAGGTG+GGG + chr5_2:8180113-8180132 None:intergenic 50.0%
GCTCTTTCAGCCATTGTTCC+TGG - chr5_2:8180824-8180843 Msa0754590:intron 50.0%
ACAAGCACGTTTGCAGACAC+TGG - chr5_2:8181535-8181554 Msa0754590:intron 50.0%
! TAATTGGCCAAGCACCGTAG+CGG + chr5_2:8181750-8181769 None:intergenic 50.0%
!!! TTTTTTGTGGTGGCCGAGGT+TGG - chr5_2:8182227-8182246 Msa0754590:CDS 50.0%
TGTGACAGCAAAGGATCTCC+TGG - chr5_2:8182455-8182474 Msa0754590:CDS 50.0%
GTGACAGCAAAGGATCTCCT+GGG - chr5_2:8182456-8182475 Msa0754590:CDS 50.0%
CATCACAGGACCTTCCTTCA+TGG + chr5_2:8182636-8182655 None:intergenic 50.0%
CATGAAGGAAGGTCCTGTGA+TGG - chr5_2:8182634-8182653 Msa0754590:intron 50.0%
TCGAGCTTGAGCAGTGTTCC+TGG + chr5_2:8180750-8180769 None:intergenic 55.0%
AGCACGTTTGCAGACACTGG+AGG - chr5_2:8181538-8181557 Msa0754590:intron 55.0%
! TCTCCTGGGAAGCATACGAC+AGG - chr5_2:8182470-8182489 Msa0754590:CDS 55.0%
AAGCCTGTCGTATGCTTCCC+AGG + chr5_2:8182476-8182495 None:intergenic 55.0%
! ACATGTTCTGGGTCGGTGTG+TGG - chr5_2:8182735-8182754 Msa0754590:intron 55.0%
! ACACAGTGGACGTGCCGCTA+CGG - chr5_2:8181733-8181752 Msa0754590:intron 60.0%
! GGACGTGCCGCTACGGTGCT+TGG - chr5_2:8181740-8181759 Msa0754590:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 8179815 8183065 8179815 ID=Msa0754590;Name=Msa0754590
chr5_2 mRNA 8179815 8183065 8179815 ID=Msa0754590-mRNA-1;Parent=Msa0754590;Name=Msa0754590-mRNA-1;_AED=0.06;_eAED=0.06;_QI=74|1|1|1|0.75|0.6|5|553|345
chr5_2 exon 8179815 8180442 8179815 ID=Msa0754590-mRNA-1:exon:3761;Parent=Msa0754590-mRNA-1
chr5_2 exon 8181030 8181395 8181030 ID=Msa0754590-mRNA-1:exon:3760;Parent=Msa0754590-mRNA-1
chr5_2 exon 8182024 8182530 8182024 ID=Msa0754590-mRNA-1:exon:3759;Parent=Msa0754590-mRNA-1
chr5_2 exon 8182749 8182841 8182749 ID=Msa0754590-mRNA-1:exon:3758;Parent=Msa0754590-mRNA-1
chr5_2 exon 8182995 8183065 8182995 ID=Msa0754590-mRNA-1:exon:3757;Parent=Msa0754590-mRNA-1
chr5_2 five_prime_UTR 8182995 8183065 8182995 ID=Msa0754590-mRNA-1:five_prime_utr;Parent=Msa0754590-mRNA-1
chr5_2 five_prime_UTR 8182839 8182841 8182839 ID=Msa0754590-mRNA-1:five_prime_utr;Parent=Msa0754590-mRNA-1
chr5_2 CDS 8182749 8182838 8182749 ID=Msa0754590-mRNA-1:cds;Parent=Msa0754590-mRNA-1
chr5_2 CDS 8182024 8182530 8182024 ID=Msa0754590-mRNA-1:cds;Parent=Msa0754590-mRNA-1
chr5_2 CDS 8181030 8181395 8181030 ID=Msa0754590-mRNA-1:cds;Parent=Msa0754590-mRNA-1
chr5_2 CDS 8180368 8180442 8180368 ID=Msa0754590-mRNA-1:cds;Parent=Msa0754590-mRNA-1
chr5_2 three_prime_UTR 8179815 8180367 8179815 ID=Msa0754590-mRNA-1:three_prime_utr;Parent=Msa0754590-mRNA-1
Gene Sequence

>Msa0754590

ATGTCCCATACCACATCTAATCAAATCGAAGTTTTAGGAAAGAAACTGAAGATGATGCAAATTATGTCCCCCACCTATGTGCCTGAACTTGGAATGGAGGATCTCAACTTCTTTAACCAATACCCGATGGATTCTCTTGCATCTCCAATATTATTCGACAACTTTGGCTTTCACTTTGATGACAACGAAACCACACCAAATTGTTTCCCAGTTGAAACTCATCTTGATGATCAAACAAGGCCAACCAAGAAGATCAAGACATCGATAAACCCTTCTTCCTATTCATCACCTCAACTCATTTCTTTTGAACACTCCAGTTCAACACCAATTGCTTCTAAGAAATTTTATAACCTTGATTATTCCGATGTAAAACCTAAAGTCGAGAAAAGGTGTAATGATAACAAAGATTTTCTTCCAGCTTTGGTTTCTCAAGGTTCTTATGAAGATCAAAAAATATTTTCAAATTATGATAACCAAGTGAATCAGACCAGGAACACTGCTCAAGCTCGAGAACATGTAATGGCCGAAAGAAAACGAAGGGAAAAGCTTACCCGAAGTTTCATCGCTCTTTCAGCCATTGTTCCTGGCCTCAAAAAGATGGACAAAGCTTCTGTGTTGGGAGATGCTATCAAGTACATGAAACAACTACAAGCACGTTTGCAGACACTGGAGGAACAAGCTGAAGACAACAAAAAAACTGGATCCACAGTTCAAGTGAAGAGATCTATTATCTTCACTAATGATGACGATGATGATGATGATAGCAACTCCAATAACCAACCACTTCCTGAAATCGAAGTAAGAGTATCAAGCAAAGATGTACTCATCAAGATTCAATGTGACAAACACAGTGGACGTGCCGCTACGGTGCTTGGCCAATTAGAAAACCTTAATCTCACTGTCCAAAGTAGTACCTTTTTGCCCTTTGGAAACAACATTGTTGATGTAACCATTGTTGCTAAGAGGAACAAGGAAAACTGTGTGACAGCAAAGGATCTCCTGGGAAGCATACGACAGGCTTTAATCATCCAAAATTAA
Protein sequence

>Msa0754590

MSHTTSNQIEVLGKKLKMMQIMSPTYVPELGMEDLNFFNQYPMDSLASPILFDNFGFHFDDNETTPNCFPVETHLDDQTRPTKKIKTSINPSSYSSPQLISFEHSSSTPIASKKFYNLDYSDVKPKVEKRCNDNKDFLPALVSQGSYEDQKIFSNYDNQVNQTRNTAQAREHVMAERKRREKLTRSFIALSAIVPGLKKMDKASVLGDAIKYMKQLQARLQTLEEQAEDNKKTGSTVQVKRSIIFTNDDDDDDDSNSNNQPLPEIEVRVSSKDVLIKIQCDKHSGRAATVLGQLENLNLTVQSSTFLPFGNNIVDVTIVAKRNKENCVTAKDLLGSIRQALIIQN*