AlfalfaGEDB | Msa0713540

M. sativa cultivar zhongmu-4 / Msa0713540

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0008283 GO:0009719 GO:0009725 GO:0009733 GO:0009787 GO:0009789 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0019219 GO:0019222 GO:0023051 GO:0023056 GO:0031323 GO:0031326 GO:0032870 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901419 GO:1901421 GO:1903506 GO:1905957 GO:1905959 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0713540	QSD99859.1	95.821	335	14	0	1	335	1	335	0.0	675

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0713540	sp\|O49697\|NAC71_ARATH	57.778	270	79	7	1	264	1	241	4.74e-96	288

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0713540	A0A896WBT5	95.821	335	14	0	1	335	1	335	0.0	675

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TFs/TRs:

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Gene ID	Type	Classification
Msa0713540	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0713540	Msa0755580	0.970119	3.907155e-131	-8.615850e-47
Msa0713540	Msa0797230	0.932775	5.124911e-95	-8.615850e-47
Msa0713540	Msa0835020	0.978236	2.100922e-145	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0713540	MtrunA17_Chr5g0400461	96.119	335	13	0	1	335	1	335	0.0	674
Msa0713540	MtrunA17_Chr4g0054791	60.290	345	100	9	1	335	1	318	7.21e-132	378
Msa0713540	MtrunA17_Chr6g0481091	62.500	176	56	2	1	173	1	169	3.13e-74	229
Msa0713540	MtrunA17_Chr3g0109821	64.024	164	48	2	1	160	1	157	4.58e-70	230
Msa0713540	MtrunA17_Chr5g0433781	63.804	163	49	2	1	160	1	156	7.67e-67	221
Msa0713540	MtrunA17_Chr2g0309891	46.930	228	102	3	1	216	1	221	8.07e-67	213
Msa0713540	MtrunA17_Chr3g0128151	53.529	170	69	3	5	171	21	183	5.23e-53	183
Msa0713540	MtrunA17_Chr5g0428991	53.704	162	65	3	6	164	25	179	1.39e-52	183
Msa0713540	MtrunA17_Chr1g0201021	55.769	156	59	2	8	160	21	169	1.79e-52	181
Msa0713540	MtrunA17_Chr3g0128111	52.201	159	66	2	9	164	45	196	4.42e-52	172

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0713540	AT4G17980.2	52.632	323	108	10	1	315	1	286	4.52e-98	292
Msa0713540	AT4G17980.1	57.778	270	79	7	1	264	1	241	4.82e-97	288
Msa0713540	AT5G46590.1	54.982	271	92	7	1	266	1	246	6.00e-93	279
Msa0713540	AT1G32510.1	65.534	206	62	4	1	202	1	201	1.39e-86	262
Msa0713540	AT5G46590.2	53.731	268	89	7	1	266	1	235	1.87e-86	262
Msa0713540	AT1G65910.1	64.162	173	52	2	1	170	1	166	2.59e-71	233
Msa0713540	AT5G17260.1	46.809	282	119	7	1	267	1	266	2.77e-70	226
Msa0713540	AT1G54330.1	65.217	161	49	1	1	161	1	154	1.89e-69	219
Msa0713540	AT3G17730.1	63.030	165	49	3	1	161	1	157	4.52e-69	216
Msa0713540	AT3G03200.1	65.244	164	46	3	1	160	1	157	1.05e-68	223

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Find 205 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAAACCAAAGAGAAAG+TGG	0.490273	5_1:+5721333	None:intergenic
AAAATTGGATCTTGTTATTG+AGG	0.463462	5_1:+5718154	Msa0713530:CDS
AAACTAGCTGCTGCACAAGT+TGG	0.544612	5_1:+5717906	Msa0713530:CDS
AAACTTCCTTGTCCTGAATA+AGG	0.309664	5_1:+5718929	None:intergenic
AAAGATGCAAAAGCAGCAAA+AGG	0.479326	5_1:-5718113	Msa0713540:three_prime_UTR
AAATTTGAGTAGGATGAACA+AGG	0.615587	5_1:+5719004	None:intergenic
AACATGGCTCTAACTGATCT+TGG	0.468885	5_1:-5717675	Msa0713540:three_prime_UTR
AACCTCGAGTTGATGTGTCG+CGG	0.672079	5_1:+5718252	Msa0713530:CDS
AACTAGCAGGACTATTCTCA+AGG	0.550921	5_1:-5719121	Msa0713540:intron
AAGAATCAAGAATCATGTCA+TGG	0.536644	5_1:+5719422	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTGTGTAATATTCCTAAGTT+TGG	-	chr5_1:5717148-5717167	Msa0713540:three_prime_UTR	30.0%
GGAAAAAATGTACCAAACTT+AGG	+	chr5_1:5717163-5717182	Msa0713530:CDS	30.0%
AGAGTTAAATGACACTTCAA+CGG	+	chr5_1:5717265-5717284	Msa0713530:intron	30.0%
ACATAGAAATATAGAATGGG+AGG	-	chr5_1:5717434-5717453	Msa0713540:three_prime_UTR	30.0%
ATATCCTATCAACTCTTCAT+CGG	+	chr5_1:5717495-5717514	Msa0713530:intron	30.0%
ACAACAAATAGGAGGATTTA+AGG	-	chr5_1:5717950-5717969	Msa0713540:three_prime_UTR	30.0%
TATTTGTCCTTGAACCATTT+GGG	+	chr5_1:5718348-5718367	None:intergenic	30.0%
TCTTCCACAGTAAAAAACTA+GGG	+	chr5_1:5718476-5718495	None:intergenic	30.0%
TTCTTATTGATCACTGTTGT+AGG	-	chr5_1:5718657-5718676	Msa0713540:three_prime_UTR	30.0%
TCTTATTGATCACTGTTGTA+GGG	-	chr5_1:5718658-5718677	Msa0713540:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	5717019	5721367	5717019	ID=Msa0713540;Name=Msa0713540
chr5_1	mRNA	5717019	5721367	5717019	ID=Msa0713540-mRNA-1;Parent=Msa0713540;Name=Msa0713540-mRNA-1;_AED=0.12;_eAED=0.12;_QI=430\|1\|1\|1\|0.75\|0.6\|5\|1755\|335
chr5_1	exon	5717019	5717185	5717019	ID=Msa0713540-mRNA-1:exon:2534;Parent=Msa0713540-mRNA-1
chr5_1	exon	5717277	5719134	5717277	ID=Msa0713540-mRNA-1:exon:2533;Parent=Msa0713540-mRNA-1
chr5_1	exon	5719417	5719707	5719417	ID=Msa0713540-mRNA-1:exon:2532;Parent=Msa0713540-mRNA-1
chr5_1	exon	5719816	5720105	5719816	ID=Msa0713540-mRNA-1:exon:2531;Parent=Msa0713540-mRNA-1
chr5_1	exon	5720781	5721367	5720781	ID=Msa0713540-mRNA-1:exon:2530;Parent=Msa0713540-mRNA-1
chr5_1	five_prime_UTR	5720938	5721367	5720938	ID=Msa0713540-mRNA-1:five_prime_utr;Parent=Msa0713540-mRNA-1
chr5_1	CDS	5720781	5720937	5720781	ID=Msa0713540-mRNA-1:cds;Parent=Msa0713540-mRNA-1
chr5_1	CDS	5719816	5720105	5719816	ID=Msa0713540-mRNA-1:cds;Parent=Msa0713540-mRNA-1
chr5_1	CDS	5719417	5719707	5719417	ID=Msa0713540-mRNA-1:cds;Parent=Msa0713540-mRNA-1
chr5_1	CDS	5718865	5719134	5718865	ID=Msa0713540-mRNA-1:cds;Parent=Msa0713540-mRNA-1
chr5_1	three_prime_UTR	5717277	5718864	5717277	ID=Msa0713540-mRNA-1:three_prime_utr;Parent=Msa0713540-mRNA-1
chr5_1	three_prime_UTR	5717019	5717185	5717019	ID=Msa0713540-mRNA-1:three_prime_utr;Parent=Msa0713540-mRNA-1

Gene Sequence

>Msa0713540

ATGGGAGGAGCTTCACTGCCTCCAGGTTTTCGTTTCCATCCAACCGATGAAGAGTTGATAGGATATTACCTAAAAAGAAAAGTTGAGGGACTTGAAATTGAGCTTGAAGTTATACCTGTTATTGAATTCTACAAATTTGATCCTTGGGAGTTACCTGAGAAATCTTTCCTGCCAAAAAGAGATATGGAATGGTTTTTCTTCTGTCCACGGGATCGCAAGTACCCAAATGGTTCAAGGACAAATAGAGCCACCAAAGCTGGCTACTGGAAAGCCACAGGAAAAGACAGGAAGGTTGTGTGTGAGCCTAGTCCATTCATTTCCACTGAGACTGTCACAGGTTACCGCAAGACCCTAGTTTTTTACTGTGGAAGAGCTCCTTTAGGTGATCGAACCAATTGGGTCATGCATGAATATCGCCTCAATGATGATCCTAGCCAAGGCTCGGAGGGTGCTTTTGCTTTATGCCGAGTTATTAAAAAGAATGAGAAGGCAAATGATTCCGAAGGACAAGGAGGAAAGAGGGTTAGAGCTAATGATGGGAGCAATTCCATTTCAACCAATGGGAACGAGGTTTCTTGTCAGGCAAGTCAACTATTGAGTGGCAGTGAGAGTCATTATTCAAGTCCTATTAACTTCCAATGCAATGTCCCTCCTATGGCTGGATTTGACCAAGCTTCCTCTGATACCAATCCTACAACCTTCTGGCTCTCCCATGACATGATTCTTGATTCTTCAAAGGACTATTCTCAAGGGCAATTACAAGATGTTGTTGGATGTTTTCCACAGCAACATGAGTTATCGAGTACAATCTCAACTTGGCAATCATTTCAACATACTGAAATTTCACCTTGTTCATCCTACTCAAATTTTAATGCGGAAATTGAATTTCCTGACACTCCTAGCCAAATTGGTTGCATGTCACCTTATTCAGGACAAGGAAGTTTCATGGACTTGCCTTATGAATGCTATGATCAGATTCATTCATTCAGGAATCCAAATCAATTCTGA

Protein sequence

>Msa0713540

MGGASLPPGFRFHPTDEELIGYYLKRKVEGLEIELEVIPVIEFYKFDPWELPEKSFLPKRDMEWFFFCPRDRKYPNGSRTNRATKAGYWKATGKDRKVVCEPSPFISTETVTGYRKTLVFYCGRAPLGDRTNWVMHEYRLNDDPSQGSEGAFALCRVIKKNEKANDSEGQGGKRVRANDGSNSISTNGNEVSCQASQLLSGSESHYSSPINFQCNVPPMAGFDQASSDTNPTTFWLSHDMILDSSKDYSQGQLQDVVGCFPQQHELSSTISTWQSFQHTEISPCSSYSNFNAEIEFPDTPSQIGCMSPYSGQGSFMDLPYECYDQIHSFRNPNQF*