AlfalfaGEDB | Msa0755580

M. sativa cultivar zhongmu-4 / Msa0755580

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0008283 GO:0009719 GO:0009725 GO:0009733 GO:0009787 GO:0009789 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010646 GO:0010647 GO:0019219 GO:0019222 GO:0023051 GO:0023056 GO:0031323 GO:0031326 GO:0032870 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901419 GO:1901421 GO:1903506 GO:1905957 GO:1905959 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0755580	sp\|O49697\|NAC71_ARATH	57.778	270	79	7	1	264	1	241	4.74e-96	288

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0755580	A0A896WBT5	95.821	335	14	0	1	335	1	335	0.0	675

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TFs/TRs:

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Gene ID	Type	Classification
Msa0755580	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0713540	Msa0755580	0.970119	3.907155e-131	-8.615850e-47
Msa0755580	Msa0797230	0.934220	5.659871e-96	-8.615850e-47
Msa0755580	Msa0835020	0.954917	1.000309e-112	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0755580	MtrunA17_Chr5g0400461	96.119	335	13	0	1	335	1	335	0.0	674
Msa0755580	MtrunA17_Chr4g0054791	60.290	345	100	9	1	335	1	318	7.21e-132	378
Msa0755580	MtrunA17_Chr6g0481091	62.500	176	56	2	1	173	1	169	3.13e-74	229
Msa0755580	MtrunA17_Chr3g0109821	64.024	164	48	2	1	160	1	157	4.58e-70	230
Msa0755580	MtrunA17_Chr5g0433781	63.804	163	49	2	1	160	1	156	7.67e-67	221
Msa0755580	MtrunA17_Chr2g0309891	46.930	228	102	3	1	216	1	221	8.07e-67	213
Msa0755580	MtrunA17_Chr3g0128151	53.529	170	69	3	5	171	21	183	5.23e-53	183
Msa0755580	MtrunA17_Chr5g0428991	53.704	162	65	3	6	164	25	179	1.39e-52	183
Msa0755580	MtrunA17_Chr1g0201021	55.769	156	59	2	8	160	21	169	1.79e-52	181
Msa0755580	MtrunA17_Chr3g0128111	52.201	159	66	2	9	164	45	196	4.42e-52	172

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0755580	AT4G17980.2	52.632	323	108	10	1	315	1	286	4.52e-98	292
Msa0755580	AT4G17980.1	57.778	270	79	7	1	264	1	241	4.82e-97	288
Msa0755580	AT5G46590.1	54.982	271	92	7	1	266	1	246	6.00e-93	279
Msa0755580	AT1G32510.1	65.534	206	62	4	1	202	1	201	1.39e-86	262
Msa0755580	AT5G46590.2	53.731	268	89	7	1	266	1	235	1.87e-86	262
Msa0755580	AT1G65910.1	64.162	173	52	2	1	170	1	166	2.59e-71	233
Msa0755580	AT5G17260.1	46.809	282	119	7	1	267	1	266	2.77e-70	226
Msa0755580	AT1G54330.1	65.217	161	49	1	1	161	1	154	1.89e-69	219
Msa0755580	AT3G17730.1	63.030	165	49	3	1	161	1	157	4.52e-69	216
Msa0755580	AT3G03200.1	65.244	164	46	3	1	160	1	157	1.05e-68	223

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Find 205 sgRNAs with CRISPR-Local

Find 298 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAAACCAAAGAGAAAG+TGG	0.490273	5_2:-9407523	None:intergenic
AAAATTGGATCTTGTTATTG+AGG	0.463462	5_2:-9410701	Msa0755590:CDS
AAACTAGCTGCTGCACAAGT+TGG	0.544612	5_2:-9410949	Msa0755590:CDS
AAACTTCCTTGTCCTGAATA+AGG	0.309664	5_2:-9409926	None:intergenic
AAAGATGCAAAAGCAGCAAA+AGG	0.479326	5_2:+9410742	Msa0755580:three_prime_UTR
AAATTTGAGTAGGATGAACA+AGG	0.615587	5_2:-9409851	None:intergenic
AACATGGCTCTAACTGATCT+TGG	0.468885	5_2:+9411180	Msa0755580:three_prime_UTR
AACCTCGAGTTGATGTGTCG+CGG	0.672079	5_2:-9410603	Msa0755590:CDS
AACTAGCAGGACTATTCTCA+AGG	0.550921	5_2:+9409734	Msa0755580:intron
AAGAATCAAGAATCATGTCA+TGG	0.536644	5_2:-9409433	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTGTGTAATATTCCTAAGTT+TGG	+	chr5_2:9407640-9407659	Msa0755580:five_prime_UTR	30.0%
GGAAAAAATGTACCAAACTT+AGG	-	chr5_2:9407655-9407674	None:intergenic	30.0%
AGAGTTAAATGACACTTCAA+CGG	-	chr5_2:9407757-9407776	None:intergenic	30.0%
ACATAGAAATATAGAATGGG+AGG	+	chr5_2:9407926-9407945	Msa0755580:exon	30.0%
ATATCCTATCAACTCTTCAT+CGG	-	chr5_2:9407987-9408006	None:intergenic	30.0%
ACAACAAATAGGAGGATTTA+AGG	+	chr5_2:9408442-9408461	Msa0755580:intron	30.0%
TATTTGTCCTTGAACCATTT+GGG	-	chr5_2:9408839-9408858	None:intergenic	30.0%
TCTTCCACAGTAAAAAACTA+GGG	-	chr5_2:9408967-9408986	None:intergenic	30.0%
TTCTTATTGATCACTGTTGT+AGG	+	chr5_2:9409148-9409167	Msa0755580:intron	30.0%
TCTTATTGATCACTGTTGTA+GGG	+	chr5_2:9409149-9409168	Msa0755580:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	9407511	9411858	9407511	ID=Msa0755580;Name=Msa0755580
chr5_2	mRNA	9407511	9411858	9407511	ID=Msa0755580-mRNA-1;Parent=Msa0755580;Name=Msa0755580-mRNA-1;_AED=0.12;_eAED=0.12;_QI=430\|1\|1\|1\|0.75\|0.6\|5\|1755\|335
chr5_2	exon	9407511	9408097	9407511	ID=Msa0755580-mRNA-1:exon:4372;Parent=Msa0755580-mRNA-1
chr5_2	exon	9408772	9409061	9408772	ID=Msa0755580-mRNA-1:exon:4373;Parent=Msa0755580-mRNA-1
chr5_2	exon	9409170	9409460	9409170	ID=Msa0755580-mRNA-1:exon:4374;Parent=Msa0755580-mRNA-1
chr5_2	exon	9409743	9411600	9409743	ID=Msa0755580-mRNA-1:exon:4375;Parent=Msa0755580-mRNA-1
chr5_2	exon	9411692	9411858	9411692	ID=Msa0755580-mRNA-1:exon:4376;Parent=Msa0755580-mRNA-1
chr5_2	five_prime_UTR	9407511	9407940	9407511	ID=Msa0755580-mRNA-1:five_prime_utr;Parent=Msa0755580-mRNA-1
chr5_2	CDS	9407941	9408097	9407941	ID=Msa0755580-mRNA-1:cds;Parent=Msa0755580-mRNA-1
chr5_2	CDS	9408772	9409061	9408772	ID=Msa0755580-mRNA-1:cds;Parent=Msa0755580-mRNA-1
chr5_2	CDS	9409170	9409460	9409170	ID=Msa0755580-mRNA-1:cds;Parent=Msa0755580-mRNA-1
chr5_2	CDS	9409743	9410012	9409743	ID=Msa0755580-mRNA-1:cds;Parent=Msa0755580-mRNA-1
chr5_2	three_prime_UTR	9410013	9411600	9410013	ID=Msa0755580-mRNA-1:three_prime_utr;Parent=Msa0755580-mRNA-1
chr5_2	three_prime_UTR	9411692	9411858	9411692	ID=Msa0755580-mRNA-1:three_prime_utr;Parent=Msa0755580-mRNA-1

Gene Sequence

>Msa0755580

ATGGGAGGAGCTTCACTGCCTCCAGGTTTTCGTTTCCATCCAACCGATGAAGAGTTGATAGGATATTACCTAAAAAGAAAAGTTGAGGGACTTGAAATTGAGCTTGAAGTTATACCTGTTATTGAATTCTACAAATTTGATCCTTGGGAGTTACCTGAGAAATCTTTCCTGCCAAAAAGAGATATGGAATGGTTTTTCTTCTGTCCACGGGATCGCAAGTACCCAAATGGTTCAAGGACAAATAGAGCCACCAAAGCTGGCTACTGGAAAGCCACAGGAAAAGACAGGAAGGTTGTGTGTGAGCCTAGTCCATTCATTTCCACTGAGACTGTCACAGGTTACCGCAAGACCCTAGTTTTTTACTGTGGAAGAGCTCCTTTAGGTGATCGAACCAATTGGGTCATGCATGAATATCGCCTCAATGATGATCCTAGCCAAGGCTCGGAGGGTGCTTTTGCTTTATGCCGAGTTATTAAAAAGAATGAGAAGGCAAATGATTCCGAAGGACAAGGAGGAAAGAGGGTTAGAGCTAATGATGGGAGCAATTCCATTTCAACCAATGGGAACGAGGTTTCTTGTCAGGCAAGTCAACTATTGAGTGGCAGTGAGAGTCATTATTCAAGTCCTATTAACTTCCAATGCAATGTCCCTCCTATGGCTGGATTTGACCAAGCTTCCTCTGATACCAATCCTACAACCTTCTGGCTCTCCCATGACATGATTCTTGATTCTTCAAAGGACTATTCTCAAGGGCAATTACAAGATGTTGTTGGATGTTTTCCACAGCAACATGAGTTATCGAGTACAATCTCAACTTGGCAATCATTTCAACATACTGAAATTTCACCTTGTTCATCCTACTCAAATTTTAATGCGGAAATTGAATTTCCTGACACTCCTAGCCAAATTGGTTGCATGTCACCTTATTCAGGACAAGGAAGTTTCATGGACTTGCCTTATGAATGCTATGATCAGATTCATTCATTCAGGAATCCAAATCAATTCTGA

Protein sequence

>Msa0755580

MGGASLPPGFRFHPTDEELIGYYLKRKVEGLEIELEVIPVIEFYKFDPWELPEKSFLPKRDMEWFFFCPRDRKYPNGSRTNRATKAGYWKATGKDRKVVCEPSPFISTETVTGYRKTLVFYCGRAPLGDRTNWVMHEYRLNDDPSQGSEGAFALCRVIKKNEKANDSEGQGGKRVRANDGSNSISTNGNEVSCQASQLLSGSESHYSSPINFQCNVPPMAGFDQASSDTNPTTFWLSHDMILDSSKDYSQGQLQDVVGCFPQQHELSSTISTWQSFQHTEISPCSSYSNFNAEIEFPDTPSQIGCMSPYSGQGSFMDLPYECYDQIHSFRNPNQF*