AlfalfaGEDB | Msa0715370

M. sativa cultivar zhongmu-4 / Msa0715370

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715370	RHN54067.1	95.062	324	16	0	2	325	24	347	0.0	571

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715370	sp\|F4JRB0\|HHO5_ARATH	39.295	369	156	12	6	329	12	357	1.43e-56	190

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715370	A0A396HTM4	95.062	324	16	0	2	325	24	347	0.0	571

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TFs/TRs:

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Gene ID	Type	Classification
Msa0715370	TF	GARP-G2-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0715370	Msa0772780	0.970802	3.569713e-132	-8.615850e-47
Msa0715370	Msa0798280	0.942417	7.454957e-102	-8.615850e-47
Msa0715370	Msa0836790	0.889774	1.791339e-73	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715370	MtrunA17_Chr5g0402631	95.062	324	16	0	2	325	24	347	0.0	571
Msa0715370	MtrunA17_Chr4g0045361	39.268	410	149	13	5	324	8	407	1.49e-65	212
Msa0715370	MtrunA17_Chr4g0062921	35.505	307	139	9	9	263	6	305	2.58e-40	146
Msa0715370	MtrunA17_Chr5g0422621	36.398	261	102	8	39	253	17	259	5.59e-36	133
Msa0715370	MtrunA17_Chr1g0198601	33.333	330	143	14	34	293	2	324	9.15e-36	133
Msa0715370	MtrunA17_Chr3g0137101	39.200	125	64	5	191	306	197	318	1.68e-14	74.3
Msa0715370	MtrunA17_Chr2g0291021	55.714	70	30	1	192	261	49	117	2.06e-14	73.2
Msa0715370	MtrunA17_Chr7g0254981	63.636	55	20	0	192	246	50	104	2.99e-14	72.0
Msa0715370	MtrunA17_Chr5g0417521	60.000	55	22	0	192	246	15	69	3.92e-14	72.4
Msa0715370	MtrunA17_Chr1g0197171	58.621	58	24	0	189	246	41	98	1.37e-13	70.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0715370	AT4G37180.1	40.055	362	156	11	6	329	12	350	1.92e-60	197
Msa0715370	AT4G37180.2	39.295	369	156	12	6	329	12	357	1.46e-57	190
Msa0715370	AT4G37180.3	43.985	266	103	8	6	242	12	260	1.47e-51	172
Msa0715370	AT2G03500.1	38.112	286	133	8	9	250	6	291	1.73e-42	152
Msa0715370	AT1G68670.1	35.461	282	118	7	31	256	6	279	2.77e-40	145
Msa0715370	AT1G25550.1	35.636	275	116	7	31	256	10	272	4.50e-40	144
Msa0715370	AT1G49560.1	56.154	130	37	4	180	290	171	299	6.79e-35	130
Msa0715370	AT1G25550.2	34.483	261	111	6	31	242	10	259	1.13e-33	125
Msa0715370	AT1G13300.1	85.000	60	9	0	191	250	180	239	3.98e-29	115
Msa0715370	AT1G49560.2	70.833	72	12	1	180	242	171	242	4.18e-26	105

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Find 76 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTGGGTTGATGAAAA+TGG	0.274484	5_1:-8415414	Msa0715370:CDS
AAAATTAGTTACCATCATTA+AGG	0.265474	5_1:+8415553	None:intergenic
AACCTTTGCAGCAGAGTTCC+AGG	0.347695	5_1:-8414994	Msa0715370:CDS
AAGAGTGGTGATGGTAGTTC+TGG	0.342533	5_1:-8415064	Msa0715370:CDS
AAGATGAGCAATCAGATTGC+AGG	0.425444	5_1:-8413957	Msa0715370:CDS
AAGCCATGGAAGAAACAGTA+TGG	0.440126	5_1:-8413986	Msa0715370:CDS
AAGTTTCTGAGACATGTCGT+TGG	0.646359	5_1:+8415665	None:intergenic
AATGGTGGAGGGGTATTTAT+GGG	0.317942	5_1:-8415166	Msa0715370:CDS
AATTAGGGAAAAGATGCAAG+TGG	0.615575	5_1:-8414360	Msa0715370:CDS
ACAAAGGAGGTGCTGGTCGT+CGG	0.559002	5_1:-8414969	Msa0715370:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTAGTTACCATCATTAAGG+AGG	+	chr5_1:8413846-8413865	None:intergenic	30.0%
ATTGTGTTACAGAAGAGTAA+TGG	-	chr5_1:8414215-8414234	Msa0715370:intron	30.0%
TAATGAAAATCTGTTGAAGG+AGG	-	chr5_1:8414259-8414278	Msa0715370:intron	30.0%
GCTCTGATTTATTATGGTAT+AGG	-	chr5_1:8414553-8414572	Msa0715370:intron	30.0%
CCACAATTTCAATTCCAAAT+TGG	+	chr5_1:8414629-8414648	None:intergenic	30.0%
AATCAATCTTAGTCGTTGTA+TGG	-	chr5_1:8414718-8414737	Msa0715370:intron	30.0%
AACTGTTTGATTAAGATTGG+AGG	-	chr5_1:8414914-8414933	Msa0715370:intron	30.0%
AAAAAAATTTGACCGTACAC+AGG	-	chr5_1:8414956-8414975	Msa0715370:CDS	30.0%
ATAATCGTATTAACCATGGA+TGG	+	chr5_1:8415135-8415154	None:intergenic	30.0%
ATGTGAAGAAGATACATCAA+AGG	-	chr5_1:8415314-8415333	Msa0715370:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	8413628	8415793	8413628	ID=Msa0715370;Name=Msa0715370
chr5_1	mRNA	8413628	8415793	8413628	ID=Msa0715370-mRNA-1;Parent=Msa0715370;Name=Msa0715370-mRNA-1;_AED=0.05;_eAED=0.05;_QI=21\|0.75\|0.8\|1\|1\|1\|5\|287\|333
chr5_1	exon	8413628	8414190	8413628	ID=Msa0715370-mRNA-1:exon:3475;Parent=Msa0715370-mRNA-1
chr5_1	exon	8414322	8414398	8414322	ID=Msa0715370-mRNA-1:exon:3474;Parent=Msa0715370-mRNA-1
chr5_1	exon	8414918	8415230	8414918	ID=Msa0715370-mRNA-1:exon:3473;Parent=Msa0715370-mRNA-1
chr5_1	exon	8415337	8415464	8415337	ID=Msa0715370-mRNA-1:exon:3472;Parent=Msa0715370-mRNA-1
chr5_1	exon	8415565	8415793	8415565	ID=Msa0715370-mRNA-1:exon:3471;Parent=Msa0715370-mRNA-1
chr5_1	five_prime_UTR	8415773	8415793	8415773	ID=Msa0715370-mRNA-1:five_prime_utr;Parent=Msa0715370-mRNA-1
chr5_1	CDS	8415565	8415772	8415565	ID=Msa0715370-mRNA-1:cds;Parent=Msa0715370-mRNA-1
chr5_1	CDS	8415337	8415464	8415337	ID=Msa0715370-mRNA-1:cds;Parent=Msa0715370-mRNA-1
chr5_1	CDS	8414918	8415230	8414918	ID=Msa0715370-mRNA-1:cds;Parent=Msa0715370-mRNA-1
chr5_1	CDS	8414322	8414398	8414322	ID=Msa0715370-mRNA-1:cds;Parent=Msa0715370-mRNA-1
chr5_1	CDS	8413915	8414190	8413915	ID=Msa0715370-mRNA-1:cds;Parent=Msa0715370-mRNA-1
chr5_1	three_prime_UTR	8413628	8413914	8413628	ID=Msa0715370-mRNA-1:three_prime_utr;Parent=Msa0715370-mRNA-1

Gene Sequence

>Msa0715370

ATGGAACAACAACAATTGAACTTAGATTTGACTTTAGCCTCTGTTCCAAAAACCGTTTCTCATTTCCTCAACAACGTTGTTCAAACCAACGACATGTCTCAGAAACTTTCAATGCTTGATGACTTAGTTCATAGCTTGGAAGAAGAAATGAAAAAAGTTCTTGCTTTCAAACGTGAACTCCCTCTTTCCATACTCCTCCTTAATGATGCTATAGCAAGATTGAATGATGAAAAAGAAAAAGTTGGGTTGATGAAAATGGAAGATTTGAAAGAAAACTGTGATAATAAGAAGAATTGGATGAGTTCAGCTCAGCTATGGACTAATCAAACAAAAACGAACAATGAAGGTGATGATGATAGGATTGTGTTACAGAAGAGTAATGGTGGAGGGGTATTTATGGGATTTAATGAAAATCTGTTGAAGGAGGTTTCACAAGCTAAGAGCTTTAGTTTGGTGTCTGAAGTGAGTCATGGAAATTCAAAGAGTGGTGATGGTAGTTCTGGATCGTCTTTGTTGCGTGTTGAGATACAGAATCAACCTCAGCCGCCGCAACCTTTGCAGCAGAGTTCCAGGAAACAAAGGAGGTGCTGGTCGTCGGAGCTTCATCGCCGATTTGTTGATGCACTTCAACAGCTTGGTGGAGCACATGCAGCCACTCCTAAGCAAATTAGGGAAAAGATGCAAGTGGATGGATTAACAAATGATGAAGTGAAAAGCCATTTGCAAAAGTATAGGCTTCATGTTAGAAGATTCCCAGTTTCGTCAATTCAGGAGGCTAATAAGTTAGCCTTGTATATGGCTCAAGATCAATGTGAAGAAGATACATCAAAGGGAAACTTCTCAGAGTCTGTTTCTCCACAGGGTCCTCTTACTCCTCTTCTCCTTGGTGGATCTGTTCAAGGTCTTTCAAGCCATGGAAGAAACAGTATGGATGCTGAAGATGAGCAATCAGATTGCAGGAATTGGAAAAGTGATCAGGAGCAGCAGCATGAAGCTGAATAA

Protein sequence

>Msa0715370

MEQQQLNLDLTLASVPKTVSHFLNNVVQTNDMSQKLSMLDDLVHSLEEEMKKVLAFKRELPLSILLLNDAIARLNDEKEKVGLMKMEDLKENCDNKKNWMSSAQLWTNQTKTNNEGDDDRIVLQKSNGGGVFMGFNENLLKEVSQAKSFSLVSEVSHGNSKSGDGSSGSSLLRVEIQNQPQPPQPLQQSSRKQRRCWSSELHRRFVDALQQLGGAHAATPKQIREKMQVDGLTNDEVKSHLQKYRLHVRRFPVSSIQEANKLALYMAQDQCEEDTSKGNFSESVSPQGPLTPLLLGGSVQGLSSHGRNSMDAEDEQSDCRNWKSDQEQQHEAE*