AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0721410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0721410 MtrunA17_Chr5g0412221 97.110 173 5 0 1 173 1 173 2.36e-122 342
Msa0721410 MtrunA17_Chr8g0367531 59.116 181 57 7 3 173 1 174 1.29e-48 155
Msa0721410 MtrunA17_Chr5g0427491 50.568 176 73 8 1 169 1 169 1.34e-45 147
Msa0721410 MtrunA17_Chr3g0132871 38.983 177 94 6 1 169 1 171 4.35e-26 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0721410 AT4G38060.3 44.118 136 57 4 36 171 20 136 1.82e-25 95.5
Msa0721410 AT4G38060.2 44.118 136 57 4 36 171 20 136 1.82e-25 95.5
Msa0721410 AT4G38060.4 44.118 136 57 4 36 171 20 136 1.82e-25 95.5
Msa0721410 AT4G38060.1 40.323 124 55 4 36 159 20 124 4.07e-17 73.6
Msa0721410 AT5G65480.1 42.222 90 33 5 83 168 76 150 7.51e-12 60.5

Find 78 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAGAAACAATTCCAATTT+TGG 0.118475 5_1:-17979247 Msa0721410:five_prime_UTR
ATTTCTTCAAGTTGCTTAAT+TGG 0.122561 5_1:-17979357 Msa0721410:five_prime_UTR
TTAATTGGGTTTGATATTTA+AGG 0.124236 5_1:-17979342 Msa0721410:five_prime_UTR
TCAATTTGCAATGCAAAATT+TGG 0.174380 5_1:-17976971 Msa0721410:three_prime_UTR
CTTAGCTTTAGGGTCAAAAT+TGG 0.205002 5_1:+17979634 None:intergenic
ATCTTCATCTTGTATGATTA+AGG 0.214291 5_1:-17977099 Msa0721410:three_prime_UTR
TTTGAATCATAATGAGATAT+TGG 0.219187 5_1:-17979548 Msa0721410:five_prime_UTR
GAGTAAAGACCTTAGCTTTA+GGG 0.230350 5_1:+17979624 None:intergenic
ATTATTGGGGATTAATTTCT+TGG 0.242680 5_1:-17979457 Msa0721410:five_prime_UTR
CAATTCTTTCTCAAGTTCCT+TGG 0.266048 5_1:-17979124 Msa0721410:five_prime_UTR
GCAAGAAAATATTGTAACTA+TGG 0.281433 5_1:-17978992 Msa0721410:exon
CTTTGACACGTTTGTCTTGT+TGG 0.293279 5_1:-17978963 Msa0721410:CDS
GAGAAAGAGCCTATTGTTAA+TGG 0.294492 5_1:-17978922 Msa0721410:CDS
CAATTCATAAAAGCCAAAAT+TGG 0.307937 5_1:+17979234 None:intergenic
TATCTCATTATGATTCAAAA+TGG 0.313629 5_1:+17979552 None:intergenic
TTGCTTCCTCTACTTCTAAA+AGG 0.316170 5_1:+17977063 None:intergenic
GATGACTCAGATTGGTCTAT+TGG 0.319310 5_1:-17978703 Msa0721410:CDS
TTGAATCATAATGAGATATT+GGG 0.321523 5_1:-17979547 Msa0721410:five_prime_UTR
TGAGTAAAGACCTTAGCTTT+AGG 0.328629 5_1:+17979623 None:intergenic
CTATGAGCGACGAAGCATTT+AGG 0.348762 5_1:+17979035 None:intergenic
CTAGGTTTCTTCTTGTTCAC+TGG 0.354455 5_1:-17979590 Msa0721410:five_prime_UTR
AATTCTTTCTCAAGTTCCTT+GGG 0.356156 5_1:-17979123 Msa0721410:five_prime_UTR
TTGGTTATGGAGAGGTAGTA+AGG 0.362202 5_1:-17978944 Msa0721410:CDS
AAATTACATTAGATACATAA+TGG 0.363088 5_1:+17977033 None:intergenic
CACGTTTGTCTTGTTGGTTA+TGG 0.369107 5_1:-17978957 Msa0721410:CDS
TTTCTTCAAGTTGCTTAATT+GGG 0.372764 5_1:-17979356 Msa0721410:five_prime_UTR
GATGGATTTCCATTAACAAT+AGG 0.374602 5_1:+17978913 None:intergenic
CAAATAATCAAAGGAAGGTT+AGG 0.383783 5_1:-17978828 Msa0721410:CDS
GGTGGTGGTGGTTGTTTGTC+AGG 0.396455 5_1:-17978736 Msa0721410:CDS
CTTGAAGAAATTAATTTGAC+TGG 0.402088 5_1:+17979369 None:intergenic
TTACCAGAAGAAAATTCATT+TGG 0.409882 5_1:+17978526 None:intergenic
TTAGGAAGAAGTGGCAGAGT+AGG 0.411049 5_1:-17978810 Msa0721410:CDS
TGGAGCTAGAACATACTATA+TGG 0.419313 5_1:-17976951 Msa0721410:three_prime_UTR
ATGACTCAGATTGGTCTATT+GGG 0.420028 5_1:-17978702 Msa0721410:CDS
TTTGAAGTTTCCTTTGATGA+AGG 0.436540 5_1:-17978854 Msa0721410:CDS
TAGGAAGAAGTGGCAGAGTA+GGG 0.441023 5_1:-17978809 Msa0721410:CDS
AAGATGAAGATGCGTGTGTT+TGG 0.448789 5_1:+17977111 None:intergenic
CCTTCAACTTCCTTGCAACC+TGG 0.459031 5_1:+17978589 None:intergenic
AGTGGCAGAGTAGGGAAGAG+AGG 0.465355 5_1:-17978801 Msa0721410:CDS
CTTCTAAAAGGTAGTCAGAA+AGG 0.471157 5_1:+17977075 None:intergenic
TTTACTCAGAAGTATTTACT+AGG 0.473743 5_1:-17979608 Msa0721410:five_prime_UTR
ATGGAAATCCATCTTCTGAA+TGG 0.490855 5_1:-17978903 Msa0721410:CDS
TCTCAAGTTCCTTGGGTGAG+TGG 0.495210 5_1:-17979116 Msa0721410:five_prime_UTR
CAGTTGTGTGCAGAGATATC+CGG 0.506930 5_1:-17979083 Msa0721410:five_prime_UTR
TCAACTTCAATATTCTACTT+CGG 0.510933 5_1:-17976859 Msa0721410:three_prime_UTR
CCAGGTTGCAAGGAAGTTGA+AGG 0.512670 5_1:-17978589 Msa0721410:CDS
AACTCTGATTTCCACTGCGC+CGG 0.513972 5_1:-17977184 Msa0721410:three_prime_UTR
CGATGGTCGTGGACGGTGGA+AGG 0.519400 5_1:-17977149 Msa0721410:three_prime_UTR
GTATCATCTGATGGTGGTGG+TGG 0.523059 5_1:-17978748 Msa0721410:CDS
TTGGTTCCTTGTTATAGACC+AGG 0.523146 5_1:-17978607 Msa0721410:CDS
ATTGGGGATTAATTTCTTGG+AGG 0.524342 5_1:-17979454 Msa0721410:five_prime_UTR
CTGAATCTGATGACTCAGAT+TGG 0.527691 5_1:-17978711 Msa0721410:CDS
TTGAAGTTTCCTTTGATGAA+GGG 0.535589 5_1:-17978853 Msa0721410:CDS
TGCAGCTGGAAACAATTACA+TGG 0.536763 5_1:-17977269 Msa0721410:intron
ATTGGGTGGTTAGAGCCTCA+TGG 0.551380 5_1:-17978685 Msa0721410:CDS
TTATTTGTTCCCTTCATCAA+AGG 0.553314 5_1:+17978844 None:intergenic
AAAGGAAGGTTAGGAAGAAG+TGG 0.568851 5_1:-17978819 Msa0721410:CDS
TTAGTATCATCTGATGGTGG+TGG 0.573261 5_1:-17978751 Msa0721410:CDS
TGATCGATGGTCGTGGACGG+TGG 0.573888 5_1:-17977153 Msa0721410:three_prime_UTR
TTGTTATAGACCAGGTTGCA+AGG 0.574059 5_1:-17978599 Msa0721410:CDS
TGATGCAATAAATTGATCAA+AGG 0.579494 5_1:+17979310 None:intergenic
ACCATCGATCAAACTCTAGC+CGG 0.591368 5_1:+17977165 None:intergenic
GGGAACAAATAATCAAAGGA+AGG 0.591584 5_1:-17978833 Msa0721410:CDS
GAAACAATTACATGGAACAA+TGG 0.593678 5_1:-17977261 Msa0721410:CDS
TGAAGGGAACAAATAATCAA+AGG 0.593937 5_1:-17978837 Msa0721410:CDS
GTTTGATCGATGGTCGTGGA+CGG 0.594622 5_1:-17977156 Msa0721410:three_prime_UTR
TTGTCTTGTTGGTTATGGAG+AGG 0.611884 5_1:-17978952 Msa0721410:CDS
ACGAAGCATTTAGGAAGCCA+AGG 0.612026 5_1:+17979044 None:intergenic
TAGAGTTTGATCGATGGTCG+TGG 0.612486 5_1:-17977160 Msa0721410:three_prime_UTR
ACTCAGATTGGTCTATTGGG+TGG 0.613034 5_1:-17978699 Msa0721410:CDS
ATCAGAGTTTACAAACACAC+TGG 0.616161 5_1:+17977198 None:intergenic
TCAAACTCTAGCCGGCGCAG+TGG 0.648271 5_1:+17977173 None:intergenic
AATAAATTGATCAAAGGACA+AGG 0.657144 5_1:+17979316 None:intergenic
GGAGACTAACCACTCACCCA+AGG 0.660610 5_1:+17979107 None:intergenic
GCCGGCTAGAGTTTGATCGA+TGG 0.667733 5_1:-17977166 Msa0721410:three_prime_UTR
TTGCAACCTGGTCTATAACA+AGG 0.667827 5_1:+17978601 None:intergenic
GATATCTCTGCACACAACTG+CGG 0.685040 5_1:+17979086 None:intergenic
CTCTGAAAATCAGAACCATG+AGG 0.713992 5_1:+17978670 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATCATTTTTTTTAGT+TGG - chr5_1:17976980-17976999 Msa0721410:three_prime_UTR 10.0%
!!! TGGATAATTTTTATTATTAT+TGG - chr5_1:17977005-17977024 Msa0721410:three_prime_UTR 10.0%
!!! GGATAATTTTTATTATTATT+GGG - chr5_1:17977006-17977025 Msa0721410:three_prime_UTR 10.0%
!!! GATAATTTTTATTATTATTG+GGG - chr5_1:17977007-17977026 Msa0721410:three_prime_UTR 10.0%
!!! TTTTTGTTTTTTTTTATTCT+TGG - chr5_1:17978251-17978270 Msa0721410:intron 10.0%
!!! TCATATTTCTTTGTATATTT+TGG - chr5_1:17978532-17978551 Msa0721410:CDS 15.0%
!!! TATCTATTTCTTATTTTGAA+GGG + chr5_1:17978776-17978795 None:intergenic 15.0%
!!! ATATCTATTTCTTATTTTGA+AGG + chr5_1:17978777-17978796 None:intergenic 15.0%
!! AATAGATATAAAAGATAAAC+TGG - chr5_1:17978788-17978807 Msa0721410:CDS 15.0%
!! AAATTACATTAGATACATAA+TGG + chr5_1:17979447-17979466 None:intergenic 15.0%
!!! TTTTTTAATGTAATAGTGAT+TGG + chr5_1:17979552-17979571 None:intergenic 15.0%
!! ATTAAAAAAGCAATACTATA+TGG - chr5_1:17979563-17979582 Msa0721410:five_prime_UTR 15.0%
!! TATCTCATTATGATTCAAAA+TGG + chr5_1:17976928-17976947 None:intergenic 20.0%
!! TTTGAATCATAATGAGATAT+TGG - chr5_1:17976929-17976948 Msa0721410:three_prime_UTR 20.0%
!! TTGAATCATAATGAGATATT+GGG - chr5_1:17976930-17976949 Msa0721410:three_prime_UTR 20.0%
!!! ATCATTTTTTTTAGTTGGAT+TGG - chr5_1:17976985-17977004 Msa0721410:three_prime_UTR 20.0%
!!! TTTTTGTTGACATTCAATTT+TGG - chr5_1:17977067-17977086 Msa0721410:three_prime_UTR 20.0%
!!! TTTTGTTGACATTCAATTTT+GGG - chr5_1:17977068-17977087 Msa0721410:three_prime_UTR 20.0%
!! TTAATTGGGTTTGATATTTA+AGG - chr5_1:17977135-17977154 Msa0721410:three_prime_UTR 20.0%
!!! AATAGAAACAATTCCAATTT+TGG - chr5_1:17977230-17977249 Msa0721410:exon 20.0%
!!! AAAAATTTGAGACATAGAAT+GGG + chr5_1:17977325-17977344 None:intergenic 20.0%
!!! AAAAAATTTGAGACATAGAA+TGG + chr5_1:17977326-17977345 None:intergenic 20.0%
!!! TTCTATGTCTCAAATTTTTT+TGG - chr5_1:17977326-17977345 Msa0721410:intron 20.0%
!!! ATTTTGCTATATTTGTAGTT+TGG - chr5_1:17978143-17978162 Msa0721410:intron 20.0%
!! TCATTTCTCAACAATATAAT+GGG + chr5_1:17978414-17978433 None:intergenic 20.0%
!! ATCATTTCTCAACAATATAA+TGG + chr5_1:17978415-17978434 None:intergenic 20.0%
!!! AACTGTTTTTTTCTTGTTTA+TGG - chr5_1:17978882-17978901 Msa0721410:CDS 20.0%
!! TCTTTGTACAAATCAAAATT+AGG - chr5_1:17979062-17979081 Msa0721410:five_prime_UTR 20.0%
!! CTTTGTACAAATCAAAATTA+GGG - chr5_1:17979063-17979082 Msa0721410:five_prime_UTR 20.0%
!!! TTTTACAATAAGAAGAGAAA+TGG + chr5_1:17979484-17979503 None:intergenic 20.0%
!!! TTTACTCAGAAGTATTTACT+AGG - chr5_1:17976869-17976888 Msa0721410:three_prime_UTR 25.0%
! ATTATTGGGGATTAATTTCT+TGG - chr5_1:17977020-17977039 Msa0721410:three_prime_UTR 25.0%
!! CTTGAAGAAATTAATTTGAC+TGG + chr5_1:17977111-17977130 None:intergenic 25.0%
!!! ATTTCTTCAAGTTGCTTAAT+TGG - chr5_1:17977120-17977139 Msa0721410:three_prime_UTR 25.0%
!!! TTTCTTCAAGTTGCTTAATT+GGG - chr5_1:17977121-17977140 Msa0721410:three_prime_UTR 25.0%
! AATAAATTGATCAAAGGACA+AGG + chr5_1:17977164-17977183 None:intergenic 25.0%
! TGATGCAATAAATTGATCAA+AGG + chr5_1:17977170-17977189 None:intergenic 25.0%
! CAATTCATAAAAGCCAAAAT+TGG + chr5_1:17977246-17977265 None:intergenic 25.0%
!!! CAATTTTGGCTTTTATGAAT+TGG - chr5_1:17977244-17977263 Msa0721410:CDS 25.0%
!!! TTGGTATTGATACCATTTTT+TGG - chr5_1:17977263-17977282 Msa0721410:CDS 25.0%
!!! TGGTATTGATACCATTTTTT+GGG - chr5_1:17977264-17977283 Msa0721410:CDS 25.0%
!! TATTTTCTTGCAAAGCTTAA+GGG + chr5_1:17977476-17977495 None:intergenic 25.0%
!! ATATTTTCTTGCAAAGCTTA+AGG + chr5_1:17977477-17977496 None:intergenic 25.0%
! GCAAGAAAATATTGTAACTA+TGG - chr5_1:17977485-17977504 Msa0721410:intron 25.0%
! TTACCAGAAGAAAATTCATT+TGG + chr5_1:17977954-17977973 None:intergenic 25.0%
!! AATTTTCTTCTGGTAAGATT+TGG - chr5_1:17977958-17977977 Msa0721410:intron 25.0%
!! TTTGTCATGTATTATGCTTT+TGG + chr5_1:17978221-17978240 None:intergenic 25.0%
! ATTTCTCAACAATATAATGG+GGG + chr5_1:17978412-17978431 None:intergenic 25.0%
! CATTTCTCAACAATATAATG+GGG + chr5_1:17978413-17978432 None:intergenic 25.0%
! AATGTTGAATATAAGCTCTT+TGG - chr5_1:17978680-17978699 Msa0721410:CDS 25.0%
!!! AAAGATTTTTTTTTTAGGGG+AGG + chr5_1:17978706-17978725 None:intergenic 25.0%
! TCCCCTAAAAAAAAAATCTT+TGG - chr5_1:17978705-17978724 Msa0721410:CDS 25.0%
!!! GCCAAAGATTTTTTTTTTAG+GGG + chr5_1:17978709-17978728 None:intergenic 25.0%
!!! GGCCAAAGATTTTTTTTTTA+GGG + chr5_1:17978710-17978729 None:intergenic 25.0%
!!! AGGCCAAAGATTTTTTTTTT+AGG + chr5_1:17978711-17978730 None:intergenic 25.0%
! AAGATCTATTAATACTACTC+AGG + chr5_1:17978731-17978750 None:intergenic 25.0%
! GCTTAAATCATCTTATATGA+TGG - chr5_1:17978956-17978975 Msa0721410:CDS 25.0%
! CAAATTAAGTTCAATTCTCT+TGG + chr5_1:17978983-17979002 None:intergenic 25.0%
! AACAAACAGCTCAATTAAAA+TGG + chr5_1:17979043-17979062 None:intergenic 25.0%
!! AGTGGTAATGTAATTAATGA+TGG - chr5_1:17979088-17979107 Msa0721410:five_prime_UTR 25.0%
! TGAATGTATCAAATTAGTAC+AGG + chr5_1:17979134-17979153 None:intergenic 25.0%
! TAATCCAAAAACAATAAGCA+TGG + chr5_1:17979177-17979196 None:intergenic 25.0%
! ATCTTCATCTTGTATGATTA+AGG - chr5_1:17979378-17979397 Msa0721410:five_prime_UTR 25.0%
! TCAATTTGCAATGCAAAATT+TGG - chr5_1:17979506-17979525 Msa0721410:five_prime_UTR 25.0%
! TCAACTTCAATATTCTACTT+CGG - chr5_1:17979618-17979637 Msa0721410:five_prime_UTR 25.0%
!! TGACATTCAATTTTGGGAAA+TGG - chr5_1:17977074-17977093 Msa0721410:three_prime_UTR 30.0%
!! GACATTCAATTTTGGGAAAT+GGG - chr5_1:17977075-17977094 Msa0721410:three_prime_UTR 30.0%
AATTCTTTCTCAAGTTCCTT+GGG - chr5_1:17977354-17977373 Msa0721410:intron 30.0%
!! AGGAATTTTTCAGTATGAAC+CGG + chr5_1:17977416-17977435 None:intergenic 30.0%
GTTCATACTGAAAAATTCCT+TGG - chr5_1:17977416-17977435 Msa0721410:intron 30.0%
! GATGGATTTCCATTAACAAT+AGG + chr5_1:17977567-17977586 None:intergenic 30.0%
! TTTGAAGTTTCCTTTGATGA+AGG - chr5_1:17977623-17977642 Msa0721410:intron 30.0%
TTGAAGTTTCCTTTGATGAA+GGG - chr5_1:17977624-17977643 Msa0721410:intron 30.0%
TTATTTGTTCCCTTCATCAA+AGG + chr5_1:17977636-17977655 None:intergenic 30.0%
TGAAGGGAACAAATAATCAA+AGG - chr5_1:17977640-17977659 Msa0721410:intron 30.0%
CAAATAATCAAAGGAAGGTT+AGG - chr5_1:17977649-17977668 Msa0721410:intron 30.0%
!!! GTTGTTTTAGTATCATCTGA+TGG - chr5_1:17977720-17977739 Msa0721410:intron 30.0%
!!! TGATAGTAGTTTTGCTGTTT+TGG - chr5_1:17977851-17977870 Msa0721410:intron 30.0%
! CTACCAAATGAATTTTCTTC+TGG - chr5_1:17977948-17977967 Msa0721410:intron 30.0%
TTCAAATGCATCAATCTACT+AGG - chr5_1:17978281-17978300 Msa0721410:intron 30.0%
!! TCTACTAGGTTTTTGTTTCA+TGG - chr5_1:17978295-17978314 Msa0721410:intron 30.0%
!!! TTCTAGTCTTGTGTGTTTTT+TGG - chr5_1:17978386-17978405 Msa0721410:intron 30.0%
GTATAAGTATCTGTGCAAAT+TGG + chr5_1:17978628-17978647 None:intergenic 30.0%
ATATACTTCAAACTGCAAGT+TGG - chr5_1:17979011-17979030 Msa0721410:five_prime_UTR 30.0%
TATACTTCAAACTGCAAGTT+GGG - chr5_1:17979012-17979031 Msa0721410:five_prime_UTR 30.0%
CAAATCAAAATTAGGGAAAG+TGG - chr5_1:17979070-17979089 Msa0721410:five_prime_UTR 30.0%
TACAGGAAATAGAGTTAAGA+GGG + chr5_1:17979117-17979136 None:intergenic 30.0%
AAAACAATAAGCATGGATAC+AGG + chr5_1:17979170-17979189 None:intergenic 30.0%
! GTATCCATGCTTATTGTTTT+TGG - chr5_1:17979170-17979189 Msa0721410:five_prime_UTR 30.0%
GAAACAATTACATGGAACAA+TGG - chr5_1:17979216-17979235 Msa0721410:five_prime_UTR 30.0%
!! CTTAGCTTTAGGGTCAAAAT+TGG + chr5_1:17976846-17976865 None:intergenic 35.0%
!! CAATTTTGACCCTAAAGCTA+AGG - chr5_1:17976844-17976863 Msa0721410:three_prime_UTR 35.0%
!! GAGTAAAGACCTTAGCTTTA+GGG + chr5_1:17976856-17976875 None:intergenic 35.0%
! TGAGTAAAGACCTTAGCTTT+AGG + chr5_1:17976857-17976876 None:intergenic 35.0%
ATTGGGGATTAATTTCTTGG+AGG - chr5_1:17977023-17977042 Msa0721410:three_prime_UTR 35.0%
CCATTCATGAACCCAAAAAA+TGG + chr5_1:17977278-17977297 None:intergenic 35.0%
!!! CCATTTTTTGGGTTCATGAA+TGG - chr5_1:17977275-17977294 Msa0721410:intron 35.0%
CAATTCTTTCTCAAGTTCCT+TGG - chr5_1:17977353-17977372 Msa0721410:intron 35.0%
GAGAAAGAGCCTATTGTTAA+TGG - chr5_1:17977555-17977574 Msa0721410:intron 35.0%
ATGGAAATCCATCTTCTGAA+TGG - chr5_1:17977574-17977593 Msa0721410:intron 35.0%
GAAAAACTCCATTCAGAAGA+TGG + chr5_1:17977585-17977604 None:intergenic 35.0%
GGGAACAAATAATCAAAGGA+AGG - chr5_1:17977644-17977663 Msa0721410:intron 35.0%
!! GTTTTAGTATCATCTGATGG+TGG - chr5_1:17977723-17977742 Msa0721410:intron 35.0%
!! ATGACTCAGATTGGTCTATT+GGG - chr5_1:17977775-17977794 Msa0721410:intron 35.0%
TGTGTCTGTCATTATCATCA+TGG - chr5_1:17978106-17978125 Msa0721410:intron 35.0%
GTGTCTGTCATTATCATCAT+GGG - chr5_1:17978107-17978126 Msa0721410:intron 35.0%
CTGTAAGTGCAATGATGATT+AGG - chr5_1:17978177-17978196 Msa0721410:intron 35.0%
! CAATGATGATTAGGCCTTTT+GGG - chr5_1:17978186-17978205 Msa0721410:intron 35.0%
TTTGGATTTAGCTACCCAAA+AGG + chr5_1:17978203-17978222 None:intergenic 35.0%
TGTAACTATGCATGAAACTG+TGG - chr5_1:17978344-17978363 Msa0721410:intron 35.0%
TGAAACTGTGGATACTATAG+AGG - chr5_1:17978356-17978375 Msa0721410:intron 35.0%
ACTTACTCTCTCTATTGCTT+TGG - chr5_1:17978494-17978513 Msa0721410:intron 35.0%
! CATTTTTGTTCACAGCTTGT+GGG + chr5_1:17978562-17978581 None:intergenic 35.0%
TGACAACACTAACAACATAG+AGG + chr5_1:17978602-17978621 None:intergenic 35.0%
TTGCACAGATACTTATACTC+TGG - chr5_1:17978630-17978649 Msa0721410:CDS 35.0%
TTTGAAGGGAACAAATTGCA+GGG + chr5_1:17978762-17978781 None:intergenic 35.0%
!! TTTTGAAGGGAACAAATTGC+AGG + chr5_1:17978763-17978782 None:intergenic 35.0%
GTACAGGAAATAGAGTTAAG+AGG + chr5_1:17979118-17979137 None:intergenic 35.0%
! TTACTGATTGAATTTGCAGC+TGG - chr5_1:17979194-17979213 Msa0721410:five_prime_UTR 35.0%
ATCAGAGTTTACAAACACAC+TGG + chr5_1:17979282-17979301 None:intergenic 35.0%
! CTTCTAAAAGGTAGTCAGAA+AGG + chr5_1:17979405-17979424 None:intergenic 35.0%
!! TGACTACCTTTTAGAAGTAG+AGG - chr5_1:17979408-17979427 Msa0721410:five_prime_UTR 35.0%
TTGCTTCCTCTACTTCTAAA+AGG + chr5_1:17979417-17979436 None:intergenic 35.0%
TGGAGCTAGAACATACTATA+TGG - chr5_1:17979526-17979545 Msa0721410:five_prime_UTR 35.0%
CTAGGTTTCTTCTTGTTCAC+TGG - chr5_1:17976887-17976906 Msa0721410:three_prime_UTR 40.0%
CTTTGACACGTTTGTCTTGT+TGG - chr5_1:17977514-17977533 Msa0721410:intron 40.0%
! CACGTTTGTCTTGTTGGTTA+TGG - chr5_1:17977520-17977539 Msa0721410:intron 40.0%
! TTGTCTTGTTGGTTATGGAG+AGG - chr5_1:17977525-17977544 Msa0721410:intron 40.0%
TTGGTTATGGAGAGGTAGTA+AGG - chr5_1:17977533-17977552 Msa0721410:intron 40.0%
AAAGGAAGGTTAGGAAGAAG+TGG - chr5_1:17977658-17977677 Msa0721410:intron 40.0%
!! TTAGTATCATCTGATGGTGG+TGG - chr5_1:17977726-17977745 Msa0721410:intron 40.0%
CTGAATCTGATGACTCAGAT+TGG - chr5_1:17977766-17977785 Msa0721410:intron 40.0%
!! GATGACTCAGATTGGTCTAT+TGG - chr5_1:17977774-17977793 Msa0721410:intron 40.0%
! CTCTGAAAATCAGAACCATG+AGG + chr5_1:17977810-17977829 None:intergenic 40.0%
! TTGGTTCCTTGTTATAGACC+AGG - chr5_1:17977870-17977889 Msa0721410:intron 40.0%
TTGCAACCTGGTCTATAACA+AGG + chr5_1:17977879-17977898 None:intergenic 40.0%
TTGTTATAGACCAGGTTGCA+AGG - chr5_1:17977878-17977897 Msa0721410:intron 40.0%
GACACACACATTTCTTCATC+AGG + chr5_1:17978092-17978111 None:intergenic 40.0%
! GCAATGATGATTAGGCCTTT+TGG - chr5_1:17978185-17978204 Msa0721410:intron 40.0%
!! CCATTTTTGTTCACAGCTTG+TGG + chr5_1:17978563-17978582 None:intergenic 40.0%
CCACAAGCTGTGAACAAAAA+TGG - chr5_1:17978560-17978579 Msa0721410:CDS 40.0%
TGCAGCTGGAAACAATTACA+TGG - chr5_1:17979208-17979227 Msa0721410:five_prime_UTR 40.0%
AAGATGAAGATGCGTGTGTT+TGG + chr5_1:17979369-17979388 None:intergenic 40.0%
GATATCTCTGCACACAACTG+CGG + chr5_1:17977394-17977413 None:intergenic 45.0%
CAGTTGTGTGCAGAGATATC+CGG - chr5_1:17977394-17977413 Msa0721410:intron 45.0%
! ACGAAGCATTTAGGAAGCCA+AGG + chr5_1:17977436-17977455 None:intergenic 45.0%
! CTATGAGCGACGAAGCATTT+AGG + chr5_1:17977445-17977464 None:intergenic 45.0%
TTAGGAAGAAGTGGCAGAGT+AGG - chr5_1:17977667-17977686 Msa0721410:intron 45.0%
TAGGAAGAAGTGGCAGAGTA+GGG - chr5_1:17977668-17977687 Msa0721410:intron 45.0%
!! ACTCAGATTGGTCTATTGGG+TGG - chr5_1:17977778-17977797 Msa0721410:intron 45.0%
ACCATCGATCAAACTCTAGC+CGG + chr5_1:17979315-17979334 None:intergenic 45.0%
TAGAGTTTGATCGATGGTCG+TGG - chr5_1:17979317-17979336 Msa0721410:five_prime_UTR 45.0%
TCTCAAGTTCCTTGGGTGAG+TGG - chr5_1:17977361-17977380 Msa0721410:intron 50.0%
!! GTATCATCTGATGGTGGTGG+TGG - chr5_1:17977729-17977748 Msa0721410:intron 50.0%
ATTGGGTGGTTAGAGCCTCA+TGG - chr5_1:17977792-17977811 Msa0721410:intron 50.0%
CCTTCAACTTCCTTGCAACC+TGG + chr5_1:17977891-17977910 None:intergenic 50.0%
CCAGGTTGCAAGGAAGTTGA+AGG - chr5_1:17977888-17977907 Msa0721410:intron 50.0%
AACTCTGATTTCCACTGCGC+CGG - chr5_1:17979293-17979312 Msa0721410:five_prime_UTR 50.0%
GTTTGATCGATGGTCGTGGA+CGG - chr5_1:17979321-17979340 Msa0721410:five_prime_UTR 50.0%
GGAGACTAACCACTCACCCA+AGG + chr5_1:17977373-17977392 None:intergenic 55.0%
AGTGGCAGAGTAGGGAAGAG+AGG - chr5_1:17977676-17977695 Msa0721410:intron 55.0%
!! GGTGGTGGTGGTTGTTTGTC+AGG - chr5_1:17977741-17977760 Msa0721410:intron 55.0%
GCCGGCTAGAGTTTGATCGA+TGG - chr5_1:17979311-17979330 Msa0721410:five_prime_UTR 55.0%
TCAAACTCTAGCCGGCGCAG+TGG + chr5_1:17979307-17979326 None:intergenic 60.0%
TGATCGATGGTCGTGGACGG+TGG - chr5_1:17979324-17979343 Msa0721410:five_prime_UTR 60.0%
CGATGGTCGTGGACGGTGGA+AGG - chr5_1:17979328-17979347 Msa0721410:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 17976830 17979669 17976830 ID=Msa0721410;Name=Msa0721410
chr5_1 mRNA 17976830 17979669 17976830 ID=Msa0721410-mRNA-1;Parent=Msa0721410;Name=Msa0721410-mRNA-1;_AED=0.00;_eAED=0.00;_QI=674|1|1|1|1|1|2|401|173
chr5_1 exon 17976830 17977286 17976830 ID=Msa0721410-mRNA-1:exon:6846;Parent=Msa0721410-mRNA-1
chr5_1 exon 17978530 17979669 17978530 ID=Msa0721410-mRNA-1:exon:6845;Parent=Msa0721410-mRNA-1
chr5_1 five_prime_UTR 17978996 17979669 17978996 ID=Msa0721410-mRNA-1:five_prime_utr;Parent=Msa0721410-mRNA-1
chr5_1 CDS 17978530 17978995 17978530 ID=Msa0721410-mRNA-1:cds;Parent=Msa0721410-mRNA-1
chr5_1 CDS 17977231 17977286 17977231 ID=Msa0721410-mRNA-1:cds;Parent=Msa0721410-mRNA-1
chr5_1 three_prime_UTR 17976830 17977230 17976830 ID=Msa0721410-mRNA-1:three_prime_utr;Parent=Msa0721410-mRNA-1
Gene Sequence

>Msa0721410

ATGGCTGTGTCTTTGACACGTTTGTCTTGTTGGTTATGGAGAGGTAGTAAGGAGAAAGAGCCTATTGTTAATGGAAATCCATCTTCTGAATGGAGTTTTTCATTGAAAGAAAGAGAGTCTTTGAAGTTTCCTTTGATGAAGGGAACAAATAATCAAAGGAAGGTTAGGAAGAAGTGGCAGAGTAGGGAAGAGAGGAGAGTTGATAGAGAGTATGATGTTGTTTTAGTATCATCTGATGGTGGTGGTGGTTGTTTGTCAGGCTCTGAATCTGATGACTCAGATTGGTCTATTGGGTGGTTAGAGCCTCATGGTTCTGATTTTCAGAGTGATGATGATGAGTCTGAATCTGATAGTAGTTTTGCTGTTTTGGTTCCTTGTTATAGACCAGGTTGCAAGGAAGTTGAAGGCTCAAATAATCATCTTTTGAGTGCTATCAAGAAGAATCTACCAAATGAATTTTCTTCTGCTGGAAACAATTACATGGAACAATGGTTAGCTTCACTTCAGAGTTTTGAAGCGTAG
Protein sequence

>Msa0721410

MAVSLTRLSCWLWRGSKEKEPIVNGNPSSEWSFSLKERESLKFPLMKGTNNQRKVRKKWQSREERRVDREYDVVLVSSDGGGGCLSGSESDDSDWSIGWLEPHGSDFQSDDDESESDSSFAVLVPCYRPGCKEVEGSNNHLLSAIKKNLPNEFSSAGNNYMEQWLASLQSFEA*