AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0724250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0724250 MtrunA17_Chr5g0415341 96.959 296 9 0 1 296 1 296 0.0 590
Msa0724250 MtrunA17_Chr8g0381971 39.209 278 141 8 1 266 1 262 3.14e-55 181
Msa0724250 MtrunA17_Chr4g0069121 31.276 243 151 8 27 266 34 263 1.28e-25 103
Msa0724250 MtrunA17_Chr4g0058901 27.673 318 191 14 3 295 8 311 1.35e-24 100
Msa0724250 MtrunA17_Chr4g0075211 30.000 260 149 11 23 266 34 276 3.09e-21 92.0
Msa0724250 MtrunA17_Chr2g0288891 28.244 262 151 12 23 266 20 262 8.88e-19 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0724250 AT1G10380.1 53.047 279 121 7 25 296 27 302 2.03e-96 286
Msa0724250 AT1G10380.2 57.831 249 97 6 25 268 27 272 9.27e-94 280
Msa0724250 AT3G17350.2 33.333 246 145 9 27 264 31 265 1.27e-24 100
Msa0724250 AT3G17350.1 33.333 246 145 9 27 264 31 265 1.28e-24 100
Msa0724250 AT5G50290.1 30.147 272 165 11 10 266 5 266 9.06e-23 95.9
Msa0724250 AT1G11915.1 26.421 299 181 12 10 288 14 293 1.61e-17 81.6

Find 70 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTCCAAATTCCATAAAA+AGG 0.207271 5_1:+22573206 None:intergenic
GCATAGTCAATTGAGGTAAT+AGG 0.216590 5_1:+22574498 None:intergenic
GAAGTGAGGGTCGCCGCAAC+CGG 0.279119 5_1:+22574581 None:intergenic
ACAATCTACTAAAGCGAATA+TGG 0.279440 5_1:+22574365 None:intergenic
TTCACATCATCTTACAATAA+TGG 0.287235 5_1:-22573910 Msa0724250:CDS
GTTCTTTGCCACAATTTCTT+TGG 0.298152 5_1:+22574631 None:intergenic
GTGCTATCGTCGAAAGTAAA+AGG 0.333825 5_1:+22574387 None:intergenic
CTTCAATGCAGTTCATATAC+AGG 0.342561 5_1:-22574138 Msa0724250:CDS
AATAGGACACAAGAAATAAA+TGG 0.343228 5_1:+22573331 None:intergenic
CATCATAATGATAATGAAAC+AGG 0.348890 5_1:+22573157 None:intergenic
CATAGTCAATTGAGGTAATA+GGG 0.351341 5_1:+22574499 None:intergenic
GCAAAGGCTTTGGCTTAGAT+TGG 0.353620 5_1:-22574416 Msa0724250:CDS
AGGAAGTAGGAAAAGGAAAA+TGG 0.359989 5_1:+22574677 None:intergenic
TTCTTTGGCACATTTGAGAT+TGG 0.364687 5_1:+22574646 None:intergenic
GATCCTCAAAAGTGGAACTA+TGG 0.374420 5_1:-22574087 Msa0724250:CDS
TTCGGTAGCGGCCCCGGTTG+CGG 0.383447 5_1:-22574594 Msa0724250:CDS
AGGTTAATATTAGTACTAAT+AGG 0.417556 5_1:+22574231 None:intergenic
TACCCTTTCGGTAGCGGCCC+CGG 0.428105 5_1:-22574600 Msa0724250:CDS
GTTTATACACCGGTTAATCT+TGG 0.430829 5_1:-22574189 Msa0724250:CDS
TAAACACAACATGTCGAAAT+CGG 0.436820 5_1:+22574207 None:intergenic
GTAGTAGTGTTAATTCCATT+AGG 0.443608 5_1:+22573943 None:intergenic
GCTGCATGTGAAAGGAGCTT+TGG 0.444173 5_1:-22574012 Msa0724250:CDS
TTATTTCTTGTGTCCTATTG+TGG 0.446874 5_1:-22573327 Msa0724250:three_prime_UTR
AACAAATTGATCCTCAAAAG+TGG 0.460285 5_1:-22574095 Msa0724250:CDS
AAGTGAGGGTCGCCGCAACC+GGG 0.469465 5_1:+22574582 None:intergenic
GAACCTCTCAAGTACCCTTT+CGG 0.476764 5_1:-22574612 Msa0724250:CDS
AAAGCTGGACCAAGATTAAC+CGG 0.476906 5_1:+22574180 None:intergenic
ACTTTACCTAGCAAAGGCTT+TGG 0.479182 5_1:-22574426 Msa0724250:CDS
TCAAAGAAGGAAGTAGGAAA+AGG 0.481402 5_1:+22574670 None:intergenic
GTGAAAACCTTTACAATCCT+AGG 0.482744 5_1:+22573428 None:intergenic
TTGATTTGCATAGTCAATTG+AGG 0.482998 5_1:+22574491 None:intergenic
ATTGGATCAAAGAAGGAAGT+AGG 0.483664 5_1:+22574664 None:intergenic
ATGGTGAGAAGAGGAAGTGA+AGG 0.485023 5_1:+22574696 None:intergenic
TGAGAGAATGAGGGAGGACA+AGG 0.497901 5_1:+22574742 None:intergenic
ATTTGAGATTGGATCAAAGA+AGG 0.498634 5_1:+22574657 None:intergenic
TGAAGATCCATATCAAAAGC+TGG 0.499024 5_1:+22574165 None:intergenic
TGTTGGCTGCAAGTTATATG+TGG 0.504485 5_1:+22574555 None:intergenic
ATGATCACCTAGGATTGTAA+AGG 0.507640 5_1:-22573435 Msa0724250:three_prime_UTR
ATGAGGGAGGACAAGGAATG+AGG 0.515161 5_1:+22574749 None:intergenic
AACCGGGGCCGCTACCGAAA+GGG 0.520766 5_1:+22574598 None:intergenic
CTCAAGTACCCTTTCGGTAG+CGG 0.533088 5_1:-22574606 Msa0724250:CDS
CAACCGGGGCCGCTACCGAA+AGG 0.537917 5_1:+22574597 None:intergenic
AAATCAAGAAGTTATGATGA+TGG 0.541719 5_1:-22574318 Msa0724250:CDS
TGTGATTGAGAGAATGAGGG+AGG 0.542861 5_1:+22574736 None:intergenic
AAAAGGAAAATGGTGAGAAG+AGG 0.550845 5_1:+22574687 None:intergenic
TAGGCTCATCTTATAAGCAA+TGG 0.553527 5_1:-22573476 Msa0724250:exon
CAAATGTGCCAAAGAAATTG+TGG 0.553792 5_1:-22574639 Msa0724250:CDS
GTTATATGTGGTTGGAAGTG+AGG 0.554598 5_1:+22574567 None:intergenic
TTATATGTGGTTGGAAGTGA+GGG 0.556839 5_1:+22574568 None:intergenic
TCTTGTACTTTACCTAGCAA+AGG 0.560464 5_1:-22574432 Msa0724250:CDS
TCACATGCAGCACAAGAGCT+AGG 0.560587 5_1:+22574024 None:intergenic
GACATGTTGTGTTTATACAC+CGG 0.561125 5_1:-22574199 Msa0724250:CDS
GCTCTTGTGCTGCATGTGAA+AGG 0.562347 5_1:-22574020 Msa0724250:CDS
GGCTGCAAGTTATATGTGGT+TGG 0.563811 5_1:+22574559 None:intergenic
GGAGCCTGTGTGTGTTGTGA+AGG 0.568111 5_1:+22574524 None:intergenic
GAATTTGGAATGGACTAACT+AGG 0.570768 5_1:-22573196 Msa0724250:three_prime_UTR
AGTGAGGGTCGCCGCAACCG+GGG 0.580490 5_1:+22574583 None:intergenic
TCTAAGCCAAAGCCTTTGCT+AGG 0.582371 5_1:+22574420 None:intergenic
TGGTGAGAAGAGGAAGTGAA+GGG 0.589263 5_1:+22574697 None:intergenic
CAAGAACATGTTGACATGGT+GGG 0.597427 5_1:+22574450 None:intergenic
CACAACATGTCGAAATCGGA+AGG 0.597606 5_1:+22574211 None:intergenic
CTCATCTTATAAGCAATGGA+TGG 0.598526 5_1:-22573472 Msa0724250:exon
CTCACCTTCACAACACACAC+AGG 0.609375 5_1:-22574528 Msa0724250:CDS
GTCAATTGAGGTAATAGGGT+AGG 0.619560 5_1:+22574503 None:intergenic
CTACCGAAAGGGTACTTGAG+AGG 0.634715 5_1:+22574609 None:intergenic
AATCAAGCTTATGATCACCT+AGG 0.658302 5_1:-22573445 Msa0724250:three_prime_UTR
AGTACAAGAACATGTTGACA+TGG 0.658795 5_1:+22574446 None:intergenic
ACAAGAACATGTTGACATGG+TGG 0.665001 5_1:+22574449 None:intergenic
ATCATAATGATAATGAAACA+GGG 0.694321 5_1:+22573158 None:intergenic
TTTGGAATGGACTAACTAGG+TGG 0.723775 5_1:-22573193 Msa0724250:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTTTTTTTTTTGAAA+AGG - chr5_1:22574166-22574185 Msa0724250:CDS 10.0%
!!! GTTTTTTTTTTTTTTTGAAA+AGG - chr5_1:22574166-22574185 Msa0724250:CDS 10.0%
!! TTATATAACACAAGTATTTA+AGG - chr5_1:22574144-22574163 Msa0724250:CDS 15.0%
!! TAAAAATGAATGAAAAAGTT+AGG + chr5_1:22574356-22574375 None:intergenic 15.0%
!! TTATATAACACAAGTATTTA+AGG - chr5_1:22574144-22574163 Msa0724250:CDS 15.0%
!! TAAAAATGAATGAAAAAGTT+AGG + chr5_1:22574356-22574375 None:intergenic 15.0%
!! AGGTTAATATTAGTACTAAT+AGG + chr5_1:22573693-22573712 None:intergenic 20.0%
!!! TACAATAATGGTTTTAGAAA+TGG - chr5_1:22574023-22574042 Msa0724250:CDS 20.0%
!! TGTAATTGACAAATAAGAAA+GGG + chr5_1:22574063-22574082 None:intergenic 20.0%
!! ATGTAATTGACAAATAAGAA+AGG + chr5_1:22574064-22574083 None:intergenic 20.0%
!!! ACACTAAAATTTAAGATTAG+AGG - chr5_1:22574190-22574209 Msa0724250:CDS 20.0%
!!! CACTAAAATTTAAGATTAGA+GGG - chr5_1:22574191-22574210 Msa0724250:CDS 20.0%
!!! TTTAAAACTATTATCAGTGT+CGG - chr5_1:22574285-22574304 Msa0724250:CDS 20.0%
!!! AAAAGGGTTTTTTTGTTTTT+GGG + chr5_1:22574701-22574720 None:intergenic 20.0%
!!! AAAAAGGGTTTTTTTGTTTT+TGG + chr5_1:22574702-22574721 None:intergenic 20.0%
!!! AAACAAAAAAACCCTTTTTA+TGG - chr5_1:22574703-22574722 Msa0724250:CDS 20.0%
!!! ATCATAATGATAATGAAACA+GGG + chr5_1:22574766-22574785 None:intergenic 20.0%
!! AGGTTAATATTAGTACTAAT+AGG + chr5_1:22573693-22573712 None:intergenic 20.0%
!!! TACAATAATGGTTTTAGAAA+TGG - chr5_1:22574023-22574042 Msa0724250:CDS 20.0%
!! TGTAATTGACAAATAAGAAA+GGG + chr5_1:22574063-22574082 None:intergenic 20.0%
!! ATGTAATTGACAAATAAGAA+AGG + chr5_1:22574064-22574083 None:intergenic 20.0%
!!! ACACTAAAATTTAAGATTAG+AGG - chr5_1:22574190-22574209 Msa0724250:CDS 20.0%
!!! CACTAAAATTTAAGATTAGA+GGG - chr5_1:22574191-22574210 Msa0724250:CDS 20.0%
!!! TTTAAAACTATTATCAGTGT+CGG - chr5_1:22574285-22574304 Msa0724250:CDS 20.0%
!!! AAAAGGGTTTTTTTGTTTTT+GGG + chr5_1:22574701-22574720 None:intergenic 20.0%
!!! AAAAAGGGTTTTTTTGTTTT+TGG + chr5_1:22574702-22574721 None:intergenic 20.0%
!!! AAACAAAAAAACCCTTTTTA+TGG - chr5_1:22574703-22574722 Msa0724250:CDS 20.0%
!!! ATCATAATGATAATGAAACA+GGG + chr5_1:22574766-22574785 None:intergenic 20.0%
! AAATCAAGAAGTTATGATGA+TGG - chr5_1:22573603-22573622 Msa0724250:intron 25.0%
! TTCACATCATCTTACAATAA+TGG - chr5_1:22574011-22574030 Msa0724250:CDS 25.0%
! TTAAATGTAACAAGTTGTGT+TGG + chr5_1:22574253-22574272 None:intergenic 25.0%
!!! TCATTTTTAATGACTTGTTC+TGG - chr5_1:22574367-22574386 Msa0724250:CDS 25.0%
!! TGAAGTGTATGGAATTTTAA+TGG - chr5_1:22574520-22574539 Msa0724250:CDS 25.0%
! AATAGGACACAAGAAATAAA+TGG + chr5_1:22574593-22574612 None:intergenic 25.0%
!!! AAAGGGTTTTTTTGTTTTTG+GGG + chr5_1:22574700-22574719 None:intergenic 25.0%
!! AAAACCCTTTTTATGGAATT+TGG - chr5_1:22574710-22574729 Msa0724250:CDS 25.0%
! CATTCCAAATTCCATAAAAA+GGG + chr5_1:22574717-22574736 None:intergenic 25.0%
!! CATCATAATGATAATGAAAC+AGG + chr5_1:22574767-22574786 None:intergenic 25.0%
! AAATCAAGAAGTTATGATGA+TGG - chr5_1:22573603-22573622 Msa0724250:intron 25.0%
! TTCACATCATCTTACAATAA+TGG - chr5_1:22574011-22574030 Msa0724250:CDS 25.0%
! TTAAATGTAACAAGTTGTGT+TGG + chr5_1:22574253-22574272 None:intergenic 25.0%
!!! TCATTTTTAATGACTTGTTC+TGG - chr5_1:22574367-22574386 Msa0724250:CDS 25.0%
!! TGAAGTGTATGGAATTTTAA+TGG - chr5_1:22574520-22574539 Msa0724250:CDS 25.0%
! AATAGGACACAAGAAATAAA+TGG + chr5_1:22574593-22574612 None:intergenic 25.0%
!!! AAAGGGTTTTTTTGTTTTTG+GGG + chr5_1:22574700-22574719 None:intergenic 25.0%
!! AAAACCCTTTTTATGGAATT+TGG - chr5_1:22574710-22574729 Msa0724250:CDS 25.0%
! CATTCCAAATTCCATAAAAA+GGG + chr5_1:22574717-22574736 None:intergenic 25.0%
!! CATCATAATGATAATGAAAC+AGG + chr5_1:22574767-22574786 None:intergenic 25.0%
! TTTTGTGATTGAGAGAATGA+GGG + chr5_1:22573191-22573210 None:intergenic 30.0%
! ATTTTGTGATTGAGAGAATG+AGG + chr5_1:22573192-22573211 None:intergenic 30.0%
! ATTTGAGATTGGATCAAAGA+AGG + chr5_1:22573267-22573286 None:intergenic 30.0%
CATAGTCAATTGAGGTAATA+GGG + chr5_1:22573425-22573444 None:intergenic 30.0%
!! TTGATTTGCATAGTCAATTG+AGG + chr5_1:22573433-22573452 None:intergenic 30.0%
ACAATCTACTAAAGCGAATA+TGG + chr5_1:22573559-22573578 None:intergenic 30.0%
TAAACACAACATGTCGAAAT+CGG + chr5_1:22573717-22573736 None:intergenic 30.0%
AACAAATTGATCCTCAAAAG+TGG - chr5_1:22573826-22573845 Msa0724250:intron 30.0%
! TTTTTATGCAACTGTCCTAA+TGG - chr5_1:22573963-22573982 Msa0724250:CDS 30.0%
GTAGTAGTGTTAATTCCATT+AGG + chr5_1:22573981-22574000 None:intergenic 30.0%
! TTTTCACATCTTGAAGTGTA+TGG - chr5_1:22574509-22574528 Msa0724250:CDS 30.0%
!! TGGAATTTTAATGGTTTCGA+AGG - chr5_1:22574529-22574548 Msa0724250:CDS 30.0%
!! GGAATTTTAATGGTTTCGAA+GGG - chr5_1:22574530-22574549 Msa0724250:CDS 30.0%
!! GAATTTTAATGGTTTCGAAG+GGG - chr5_1:22574531-22574550 Msa0724250:CDS 30.0%
!! AATTTTAATGGTTTCGAAGG+GGG - chr5_1:22574532-22574551 Msa0724250:CDS 30.0%
! TTATTTCTTGTGTCCTATTG+TGG - chr5_1:22574594-22574613 Msa0724250:CDS 30.0%
CCATTCCAAATTCCATAAAA+AGG + chr5_1:22574718-22574737 None:intergenic 30.0%
! CCTTTTTATGGAATTTGGAA+TGG - chr5_1:22574715-22574734 Msa0724250:exon 30.0%
! TTTTGTGATTGAGAGAATGA+GGG + chr5_1:22573191-22573210 None:intergenic 30.0%
! ATTTTGTGATTGAGAGAATG+AGG + chr5_1:22573192-22573211 None:intergenic 30.0%
! ATTTGAGATTGGATCAAAGA+AGG + chr5_1:22573267-22573286 None:intergenic 30.0%
CATAGTCAATTGAGGTAATA+GGG + chr5_1:22573425-22573444 None:intergenic 30.0%
!! TTGATTTGCATAGTCAATTG+AGG + chr5_1:22573433-22573452 None:intergenic 30.0%
ACAATCTACTAAAGCGAATA+TGG + chr5_1:22573559-22573578 None:intergenic 30.0%
TAAACACAACATGTCGAAAT+CGG + chr5_1:22573717-22573736 None:intergenic 30.0%
AACAAATTGATCCTCAAAAG+TGG - chr5_1:22573826-22573845 Msa0724250:intron 30.0%
! TTTTTATGCAACTGTCCTAA+TGG - chr5_1:22573963-22573982 Msa0724250:CDS 30.0%
GTAGTAGTGTTAATTCCATT+AGG + chr5_1:22573981-22574000 None:intergenic 30.0%
! TTTTCACATCTTGAAGTGTA+TGG - chr5_1:22574509-22574528 Msa0724250:CDS 30.0%
!! TGGAATTTTAATGGTTTCGA+AGG - chr5_1:22574529-22574548 Msa0724250:CDS 30.0%
!! GGAATTTTAATGGTTTCGAA+GGG - chr5_1:22574530-22574549 Msa0724250:CDS 30.0%
!! GAATTTTAATGGTTTCGAAG+GGG - chr5_1:22574531-22574550 Msa0724250:CDS 30.0%
!! AATTTTAATGGTTTCGAAGG+GGG - chr5_1:22574532-22574551 Msa0724250:CDS 30.0%
! TTATTTCTTGTGTCCTATTG+TGG - chr5_1:22574594-22574613 Msa0724250:CDS 30.0%
CCATTCCAAATTCCATAAAA+AGG + chr5_1:22574718-22574737 None:intergenic 30.0%
! CCTTTTTATGGAATTTGGAA+TGG - chr5_1:22574715-22574734 Msa0724250:exon 30.0%
AAAAGGAAAATGGTGAGAAG+AGG + chr5_1:22573237-22573256 None:intergenic 35.0%
AGGAAGTAGGAAAAGGAAAA+TGG + chr5_1:22573247-22573266 None:intergenic 35.0%
TCAAAGAAGGAAGTAGGAAA+AGG + chr5_1:22573254-22573273 None:intergenic 35.0%
ATTGGATCAAAGAAGGAAGT+AGG + chr5_1:22573260-22573279 None:intergenic 35.0%
! TTCTTTGGCACATTTGAGAT+TGG + chr5_1:22573278-22573297 None:intergenic 35.0%
CAAATGTGCCAAAGAAATTG+TGG - chr5_1:22573282-22573301 Msa0724250:three_prime_UTR 35.0%
GTTCTTTGCCACAATTTCTT+TGG + chr5_1:22573293-22573312 None:intergenic 35.0%
TTATATGTGGTTGGAAGTGA+GGG + chr5_1:22573356-22573375 None:intergenic 35.0%
! TTGTGAAGGTGAGTTTTTGT+TGG + chr5_1:22573386-22573405 None:intergenic 35.0%
GCATAGTCAATTGAGGTAAT+AGG + chr5_1:22573426-22573445 None:intergenic 35.0%
AGTACAAGAACATGTTGACA+TGG + chr5_1:22573478-22573497 None:intergenic 35.0%
TCTTGTACTTTACCTAGCAA+AGG - chr5_1:22573489-22573508 Msa0724250:CDS 35.0%
GACATGTTGTGTTTATACAC+CGG - chr5_1:22573722-22573741 Msa0724250:intron 35.0%
! GTTTATACACCGGTTAATCT+TGG - chr5_1:22573732-22573751 Msa0724250:intron 35.0%
TGAAGATCCATATCAAAAGC+TGG + chr5_1:22573759-22573778 None:intergenic 35.0%
CTTCAATGCAGTTCATATAC+AGG - chr5_1:22573783-22573802 Msa0724250:intron 35.0%
!! ATTCCATAGTTCCACTTTTG+AGG + chr5_1:22573840-22573859 None:intergenic 35.0%
!! GGTTGATATATGCTTTTGCA+TGG - chr5_1:22574414-22574433 Msa0724250:CDS 35.0%
! CTTTTGCATGGTCTTTGATT+AGG - chr5_1:22574426-22574445 Msa0724250:CDS 35.0%
TAGGCTCATCTTATAAGCAA+TGG - chr5_1:22574445-22574464 Msa0724250:CDS 35.0%
CTCATCTTATAAGCAATGGA+TGG - chr5_1:22574449-22574468 Msa0724250:CDS 35.0%
AATCAAGCTTATGATCACCT+AGG - chr5_1:22574476-22574495 Msa0724250:CDS 35.0%
ATGATCACCTAGGATTGTAA+AGG - chr5_1:22574486-22574505 Msa0724250:CDS 35.0%
GTGAAAACCTTTACAATCCT+AGG + chr5_1:22574496-22574515 None:intergenic 35.0%
!! GAATTTGGAATGGACTAACT+AGG - chr5_1:22574725-22574744 Msa0724250:exon 35.0%
AAAAGGAAAATGGTGAGAAG+AGG + chr5_1:22573237-22573256 None:intergenic 35.0%
AGGAAGTAGGAAAAGGAAAA+TGG + chr5_1:22573247-22573266 None:intergenic 35.0%
TCAAAGAAGGAAGTAGGAAA+AGG + chr5_1:22573254-22573273 None:intergenic 35.0%
ATTGGATCAAAGAAGGAAGT+AGG + chr5_1:22573260-22573279 None:intergenic 35.0%
! TTCTTTGGCACATTTGAGAT+TGG + chr5_1:22573278-22573297 None:intergenic 35.0%
CAAATGTGCCAAAGAAATTG+TGG - chr5_1:22573282-22573301 Msa0724250:three_prime_UTR 35.0%
GTTCTTTGCCACAATTTCTT+TGG + chr5_1:22573293-22573312 None:intergenic 35.0%
TTATATGTGGTTGGAAGTGA+GGG + chr5_1:22573356-22573375 None:intergenic 35.0%
! TTGTGAAGGTGAGTTTTTGT+TGG + chr5_1:22573386-22573405 None:intergenic 35.0%
GCATAGTCAATTGAGGTAAT+AGG + chr5_1:22573426-22573445 None:intergenic 35.0%
AGTACAAGAACATGTTGACA+TGG + chr5_1:22573478-22573497 None:intergenic 35.0%
TCTTGTACTTTACCTAGCAA+AGG - chr5_1:22573489-22573508 Msa0724250:CDS 35.0%
GACATGTTGTGTTTATACAC+CGG - chr5_1:22573722-22573741 Msa0724250:intron 35.0%
! GTTTATACACCGGTTAATCT+TGG - chr5_1:22573732-22573751 Msa0724250:intron 35.0%
TGAAGATCCATATCAAAAGC+TGG + chr5_1:22573759-22573778 None:intergenic 35.0%
CTTCAATGCAGTTCATATAC+AGG - chr5_1:22573783-22573802 Msa0724250:intron 35.0%
!! ATTCCATAGTTCCACTTTTG+AGG + chr5_1:22573840-22573859 None:intergenic 35.0%
!! GGTTGATATATGCTTTTGCA+TGG - chr5_1:22574414-22574433 Msa0724250:CDS 35.0%
! CTTTTGCATGGTCTTTGATT+AGG - chr5_1:22574426-22574445 Msa0724250:CDS 35.0%
TAGGCTCATCTTATAAGCAA+TGG - chr5_1:22574445-22574464 Msa0724250:CDS 35.0%
CTCATCTTATAAGCAATGGA+TGG - chr5_1:22574449-22574468 Msa0724250:CDS 35.0%
AATCAAGCTTATGATCACCT+AGG - chr5_1:22574476-22574495 Msa0724250:CDS 35.0%
ATGATCACCTAGGATTGTAA+AGG - chr5_1:22574486-22574505 Msa0724250:CDS 35.0%
GTGAAAACCTTTACAATCCT+AGG + chr5_1:22574496-22574515 None:intergenic 35.0%
!! GAATTTGGAATGGACTAACT+AGG - chr5_1:22574725-22574744 Msa0724250:exon 35.0%
GTTATATGTGGTTGGAAGTG+AGG + chr5_1:22573357-22573376 None:intergenic 40.0%
! TGTTGGCTGCAAGTTATATG+TGG + chr5_1:22573369-22573388 None:intergenic 40.0%
GTCAATTGAGGTAATAGGGT+AGG + chr5_1:22573421-22573440 None:intergenic 40.0%
CAAGAACATGTTGACATGGT+GGG + chr5_1:22573474-22573493 None:intergenic 40.0%
ACAAGAACATGTTGACATGG+TGG + chr5_1:22573475-22573494 None:intergenic 40.0%
ACTTTACCTAGCAAAGGCTT+TGG - chr5_1:22573495-22573514 Msa0724250:CDS 40.0%
GTGCTATCGTCGAAAGTAAA+AGG + chr5_1:22573537-22573556 None:intergenic 40.0%
AAAGCTGGACCAAGATTAAC+CGG + chr5_1:22573744-22573763 None:intergenic 40.0%
! TCTTGGTCCAGCTTTTGATA+TGG - chr5_1:22573749-22573768 Msa0724250:intron 40.0%
GATCCTCAAAAGTGGAACTA+TGG - chr5_1:22573834-22573853 Msa0724250:intron 40.0%
!!! GAAAGGAGCTTTGGATTTTG+TGG - chr5_1:22573918-22573937 Msa0724250:CDS 40.0%
!! TTCTGGCCTTTCTGTTTTCA+GGG - chr5_1:22574384-22574403 Msa0724250:CDS 40.0%
AGCAAACCCTGAAAACAGAA+AGG + chr5_1:22574393-22574412 None:intergenic 40.0%
!! TTCTGTTTTCAGGGTTTGCT+TGG - chr5_1:22574393-22574412 Msa0724250:CDS 40.0%
! GTGCACACATTTTCCACAAT+AGG + chr5_1:22574610-22574629 None:intergenic 40.0%
!! TTTGGAATGGACTAACTAGG+TGG - chr5_1:22574728-22574747 Msa0724250:exon 40.0%
GTTATATGTGGTTGGAAGTG+AGG + chr5_1:22573357-22573376 None:intergenic 40.0%
! TGTTGGCTGCAAGTTATATG+TGG + chr5_1:22573369-22573388 None:intergenic 40.0%
GTCAATTGAGGTAATAGGGT+AGG + chr5_1:22573421-22573440 None:intergenic 40.0%
CAAGAACATGTTGACATGGT+GGG + chr5_1:22573474-22573493 None:intergenic 40.0%
ACAAGAACATGTTGACATGG+TGG + chr5_1:22573475-22573494 None:intergenic 40.0%
ACTTTACCTAGCAAAGGCTT+TGG - chr5_1:22573495-22573514 Msa0724250:CDS 40.0%
GTGCTATCGTCGAAAGTAAA+AGG + chr5_1:22573537-22573556 None:intergenic 40.0%
AAAGCTGGACCAAGATTAAC+CGG + chr5_1:22573744-22573763 None:intergenic 40.0%
! TCTTGGTCCAGCTTTTGATA+TGG - chr5_1:22573749-22573768 Msa0724250:intron 40.0%
GATCCTCAAAAGTGGAACTA+TGG - chr5_1:22573834-22573853 Msa0724250:intron 40.0%
!!! GAAAGGAGCTTTGGATTTTG+TGG - chr5_1:22573918-22573937 Msa0724250:CDS 40.0%
!! TTCTGGCCTTTCTGTTTTCA+GGG - chr5_1:22574384-22574403 Msa0724250:CDS 40.0%
AGCAAACCCTGAAAACAGAA+AGG + chr5_1:22574393-22574412 None:intergenic 40.0%
!! TTCTGTTTTCAGGGTTTGCT+TGG - chr5_1:22574393-22574412 Msa0724250:CDS 40.0%
! GTGCACACATTTTCCACAAT+AGG + chr5_1:22574610-22574629 None:intergenic 40.0%
!! TTTGGAATGGACTAACTAGG+TGG - chr5_1:22574728-22574747 Msa0724250:exon 40.0%
TGTGATTGAGAGAATGAGGG+AGG + chr5_1:22573188-22573207 None:intergenic 45.0%
TGGTGAGAAGAGGAAGTGAA+GGG + chr5_1:22573227-22573246 None:intergenic 45.0%
ATGGTGAGAAGAGGAAGTGA+AGG + chr5_1:22573228-22573247 None:intergenic 45.0%
GAACCTCTCAAGTACCCTTT+CGG - chr5_1:22573309-22573328 Msa0724250:three_prime_UTR 45.0%
GGCTGCAAGTTATATGTGGT+TGG + chr5_1:22573365-22573384 None:intergenic 45.0%
TCTAAGCCAAAGCCTTTGCT+AGG + chr5_1:22573504-22573523 None:intergenic 45.0%
! GCAAAGGCTTTGGCTTAGAT+TGG - chr5_1:22573505-22573524 Msa0724250:CDS 45.0%
CACAACATGTCGAAATCGGA+AGG + chr5_1:22573713-22573732 None:intergenic 45.0%
!! GTTCTGGCCTTTCTGTTTTC+AGG - chr5_1:22574383-22574402 Msa0724250:CDS 45.0%
TGTGATTGAGAGAATGAGGG+AGG + chr5_1:22573188-22573207 None:intergenic 45.0%
TGGTGAGAAGAGGAAGTGAA+GGG + chr5_1:22573227-22573246 None:intergenic 45.0%
ATGGTGAGAAGAGGAAGTGA+AGG + chr5_1:22573228-22573247 None:intergenic 45.0%
GAACCTCTCAAGTACCCTTT+CGG - chr5_1:22573309-22573328 Msa0724250:three_prime_UTR 45.0%
GGCTGCAAGTTATATGTGGT+TGG + chr5_1:22573365-22573384 None:intergenic 45.0%
TCTAAGCCAAAGCCTTTGCT+AGG + chr5_1:22573504-22573523 None:intergenic 45.0%
! GCAAAGGCTTTGGCTTAGAT+TGG - chr5_1:22573505-22573524 Msa0724250:CDS 45.0%
CACAACATGTCGAAATCGGA+AGG + chr5_1:22573713-22573732 None:intergenic 45.0%
!! GTTCTGGCCTTTCTGTTTTC+AGG - chr5_1:22574383-22574402 Msa0724250:CDS 45.0%
!!! TTTTTTTTTTTTTTTTGAAA+AGG - chr5_1:22574110-22574129 Msa0724250:CDS 5.0%
!!! TTTTTTTTTTTTTTTTGAAA+AGG - chr5_1:22574110-22574129 Msa0724250:CDS 5.0%
ATGAGGGAGGACAAGGAATG+AGG + chr5_1:22573175-22573194 None:intergenic 50.0%
TGAGAGAATGAGGGAGGACA+AGG + chr5_1:22573182-22573201 None:intergenic 50.0%
!! CTACCGAAAGGGTACTTGAG+AGG + chr5_1:22573315-22573334 None:intergenic 50.0%
CTCAAGTACCCTTTCGGTAG+CGG - chr5_1:22573315-22573334 Msa0724250:three_prime_UTR 50.0%
CTCACCTTCACAACACACAC+AGG - chr5_1:22573393-22573412 Msa0724250:three_prime_UTR 50.0%
TCACATGCAGCACAAGAGCT+AGG + chr5_1:22573900-22573919 None:intergenic 50.0%
GCTCTTGTGCTGCATGTGAA+AGG - chr5_1:22573901-22573920 Msa0724250:CDS 50.0%
GCTGCATGTGAAAGGAGCTT+TGG - chr5_1:22573909-22573928 Msa0724250:CDS 50.0%
ATGAGGGAGGACAAGGAATG+AGG + chr5_1:22573175-22573194 None:intergenic 50.0%
TGAGAGAATGAGGGAGGACA+AGG + chr5_1:22573182-22573201 None:intergenic 50.0%
!! CTACCGAAAGGGTACTTGAG+AGG + chr5_1:22573315-22573334 None:intergenic 50.0%
CTCAAGTACCCTTTCGGTAG+CGG - chr5_1:22573315-22573334 Msa0724250:three_prime_UTR 50.0%
CTCACCTTCACAACACACAC+AGG - chr5_1:22573393-22573412 Msa0724250:three_prime_UTR 50.0%
TCACATGCAGCACAAGAGCT+AGG + chr5_1:22573900-22573919 None:intergenic 50.0%
GCTCTTGTGCTGCATGTGAA+AGG - chr5_1:22573901-22573920 Msa0724250:CDS 50.0%
GCTGCATGTGAAAGGAGCTT+TGG - chr5_1:22573909-22573928 Msa0724250:CDS 50.0%
GGAGCCTGTGTGTGTTGTGA+AGG + chr5_1:22573400-22573419 None:intergenic 55.0%
GGAGCCTGTGTGTGTTGTGA+AGG + chr5_1:22573400-22573419 None:intergenic 55.0%
! TACCCTTTCGGTAGCGGCCC+CGG - chr5_1:22573321-22573340 Msa0724250:three_prime_UTR 65.0%
AACCGGGGCCGCTACCGAAA+GGG + chr5_1:22573326-22573345 None:intergenic 65.0%
AAGTGAGGGTCGCCGCAACC+GGG + chr5_1:22573342-22573361 None:intergenic 65.0%
GAAGTGAGGGTCGCCGCAAC+CGG + chr5_1:22573343-22573362 None:intergenic 65.0%
! TACCCTTTCGGTAGCGGCCC+CGG - chr5_1:22573321-22573340 Msa0724250:three_prime_UTR 65.0%
AACCGGGGCCGCTACCGAAA+GGG + chr5_1:22573326-22573345 None:intergenic 65.0%
AAGTGAGGGTCGCCGCAACC+GGG + chr5_1:22573342-22573361 None:intergenic 65.0%
GAAGTGAGGGTCGCCGCAAC+CGG + chr5_1:22573343-22573362 None:intergenic 65.0%
CAACCGGGGCCGCTACCGAA+AGG + chr5_1:22573327-22573346 None:intergenic 70.0%
! TTCGGTAGCGGCCCCGGTTG+CGG - chr5_1:22573327-22573346 Msa0724250:three_prime_UTR 70.0%
AGTGAGGGTCGCCGCAACCG+GGG + chr5_1:22573341-22573360 None:intergenic 70.0%
CAACCGGGGCCGCTACCGAA+AGG + chr5_1:22573327-22573346 None:intergenic 70.0%
! TTCGGTAGCGGCCCCGGTTG+CGG - chr5_1:22573327-22573346 Msa0724250:three_prime_UTR 70.0%
AGTGAGGGTCGCCGCAACCG+GGG + chr5_1:22573341-22573360 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 22573153 22574790 22573153 ID=Msa0724250;Name=Msa0724250
chr5_1 mRNA 22573153 22574790 22573153 ID=Msa0724250-mRNA-1;Parent=Msa0724250;Name=Msa0724250-mRNA-1;_AED=0.00;_eAED=0.00;_QI=57|1|1|1|1|1|2|330|296
chr5_1 exon 22573153 22573538 22573153 ID=Msa0724250-mRNA-1:exon:8532;Parent=Msa0724250-mRNA-1
chr5_1 exon 22573899 22574790 22573899 ID=Msa0724250-mRNA-1:exon:8531;Parent=Msa0724250-mRNA-1
chr5_1 five_prime_UTR 22574734 22574790 22574734 ID=Msa0724250-mRNA-1:five_prime_utr;Parent=Msa0724250-mRNA-1
chr5_1 CDS 22573899 22574733 22573899 ID=Msa0724250-mRNA-1:cds;Parent=Msa0724250-mRNA-1
chr5_1 CDS 22573483 22573538 22573483 ID=Msa0724250-mRNA-1:cds;Parent=Msa0724250-mRNA-1
chr5_1 three_prime_UTR 22573153 22573482 22573153 ID=Msa0724250-mRNA-1:three_prime_utr;Parent=Msa0724250-mRNA-1
Gene Sequence

>Msa0724250

ATGAAATTTAGTTACCCTTCACTTCCTCTTCTCACCATTTTCCTTTTCCTACTTCCTTCTTTGATCCAATCTCAAATGTGCCAAAGAAATTGTGGCAAAGAACCTCTCAAGTACCCTTTCGGTAGCGGCCCCGGTTGCGGCGACCCTCACTTCCAACCACATATAACTTGCAGCCAACAAAAACTCACCTTCACAACACACACAGGCTCCTACCCTATTACCTCAATTGACTATGCAAATCAAGTCATTTACATTTCAGATCCCACCATGTCAACATGTTCTTGTACTTTACCTAGCAAAGGCTTTGGCTTAGATTGGAACGCGCCTTTTACTTTCGACGATAGCACCATATTCGCTTTAGTAGATTGTTCGATGAACTCATCATCGATATGTAAATCAAGAAGTTATGATGATGGAAGCAATTCTAAGCTTCAATGTGATCAAAATACACAAATTTGTGATGTTATGTATTCTTGTAGACCTATTAGTACTAATATTAACCTTCCGATTTCGACATGTTGTGTTTATACACCGGTTAATCTTGGTCCAGCTTTTGATATGGATCTTCAAAAGCTTCAATGCAGTTCATATACAGGTTTTTACAATTACAATGATCAACAAATTGATCCTCAAAAGTGGAACTATGGAATTGCACTTAAGTATAAGTTTAGTGTTACAAATGATTATCCTAGCTCTTGTGCTGCATGTGAAAGGAGCTTTGGATTTTGTGGATATAGTGAAGTTTATAATTCATTTTTATGCAACTGTCCTAATGGAATTAACACTACTACAGATTGTTTCTTCACATCATCTTACAATAATGGTTTTAGAAATGGGTTTGCTTGGTTGATATATGCTTTTGCATGGTCTTTGATTAGGCTCATCTTATAA
Protein sequence

>Msa0724250

MKFSYPSLPLLTIFLFLLPSLIQSQMCQRNCGKEPLKYPFGSGPGCGDPHFQPHITCSQQKLTFTTHTGSYPITSIDYANQVIYISDPTMSTCSCTLPSKGFGLDWNAPFTFDDSTIFALVDCSMNSSSICKSRSYDDGSNSKLQCDQNTQICDVMYSCRPISTNINLPISTCCVYTPVNLGPAFDMDLQKLQCSSYTGFYNYNDQQIDPQKWNYGIALKYKFSVTNDYPSSCAACERSFGFCGYSEVYNSFLCNCPNGINTTTDCFFTSSYNNGFRNGFAWLIYAFAWSLIRLIL*